Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
IHC (Immunohiostchemistry) (IHC image diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)
IHC (Immunohistochemistry) (IHC image diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)
FCM/FACS (Flow Cytometry) (Intracellular flow analysis of TLR3 in human PBMC (lymphocytes) using 2.0 ug/10^6 cells of TLR3antibody (Clone: ABM15D5). Green represents isotype control; red represents anti-TLR3 antibody. Goat anti-mouse PE conjugate was used as secondary.)
FCM/FACS (Flow Cytometry) (Intracellular flow analysis of TLR3 in human PBMC (monocytes) using 2.0 ug/10^6 cells of TLR3antibody (Clone: ABM15D5). Green represents isotype control; red represents anti-TLR3 antibody. Goat anti-mouse PE conjugate was used as secondary.)
WB (Western Blot) (Expression analysis of TLR3. Anti- TLR3 antibody (Clone: ABM15D5) was used at 2 ug/ml on Ovary lysate.)
ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-DDX3X mAb. [Lot No. 2253C5a-1])
IP (Immunoprecipitation) (Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-DDX3X mAb. [Lot No. 2253C5a-1]Predicted molecular weight: 73 kDa)
WB (Western Blot) (Detection of DDX3X by Western blot.Samples: Whole cell lysate from human HeLa (H, 25 ug), mouse NIH3T3 (M, 50 ug) and rat F2408 (R, 50 ug) cells. [Lot No. 2253C5a-1]Predicted molecular weight: 73 kDa)
Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control (Western blot analysis of immunized recombinant protein, using anti-DDX3X monoclonal antibody.)
FCM/FACS (Flow Cytometry) (Flow cytometry using the Anti-CD31 antibody BAG-85D10 . Paraformaldehyde fixed Jurkat cells were stained with anti-unknown specificity antibody or the rabbit IgG version of BAG-85D10 at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
IF (Immunofluorescence) (Immunofluorescence staining of fixed K562 cells with anti-CD31 antibody BAG-85D10 Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of BAG-85D10 at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
WB (Western Blot) (Western blot analysis of PDX1 in (1) Caco-2 cell Lysate;(2) BxPC-3 cell Lysate (AAA46950).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDX1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PDX1)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human pancreas, using PDX1 Antibody(AAA46950)PDX1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PDX1 Antibody (AAA46950)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
Western Blot, Immunocytochemistry, Immunohistochemistry, Immunofluorescence
Purity
Affinity-chromatography
Pricing
WB (Western Blot) (The recombinant proteins (10ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Adiponectin antibody (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Human Adiponectin full length (15-244aa)Lane 2.: Mouse Adiponectin globular domain (111-247aa))
WB (Western Blot) (Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of HIF1 alpha protein using Mouse Anti-HIF1 alpha Monoclonal Antibody, Clone ESEE122. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HIF1 alpha Monoclonal Antibody at 1:500 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-HIF1 alpha Monoclonal Antibody, Clone ESEE122. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-HIF1 alpha Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Membranous and cytoplasmic localization in the epidermis, positive hair follicles, muscle and dermis.)
FCM/FACS (Flow Cytometry) (Figure 4. Phylogenetic analysis of amino acid sequence of different Rabbit Anti-CS1 mAb clones. A) Heavy chain and B) Light chain.)
FCM/FACS (Flow Cytometry) (Figure 4. Phylogenetic analysis of amino acid sequence of different Rabbit Anti-CS1 mAb clones. A) Heavy chain and B) Light chain.)
Application Data (Figure 4. Phylogenetic analysis of amino acid sequence of different Rabbit Anti-CS1 mAb clones. A) Heavy chain and B) Light chain.)
Application Data (Figure 4. Phylogenetic analysis of amino acid sequence of different Rabbit Anti-CS1 mAb clones. A) Heavy chain and B) Light chain.)
IF (Immunofluorescence) (ICC/IF analysis of UNG in HeLa cells. The cell was stained with AUN0713 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
WB (Western Blot) (The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human UNG antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : HeLa cell lysate Lane 2 : Jurkat cell lysate Lane 3 : MCF7 cell lysate Lane 4 : K562 cell lysate Lane 5 : 293T cell lysate Lane 6 : HepG2 cell lysate Lane 7 : A549 cell lysate Lane 8 : SK-OV-3 cell lysate Lane 9 : PC3 cell lysate)
ICC (Immunocytochemistry) (ICC staining Caspase-3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Caspase-3 antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Caspase-3 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Caspase-3 on different cell lysates using anti-Caspase-3 antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat Lane 3: 293)
Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD14:RPE)
Application Data (Sandwich ELISA analysis of Human CD14 binding using Mouse anti CD14 antibody, clone MEM-18 as a capture reagent and biotinylated Mouse anti Human CD14 antibody, clone UCHM1 as a detection reagent with purified CD14 as antigen for generation of the standard curve. Detection is by HRP conjugated streptavidin. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . Serum samples diluted 1:200 and 1:400 are shown green and red respectively, while plasma samples also diluted 1:200 and 1:400 are blue and orange respectively)
Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD14:FITC)
Application Data (Sandwich ELISA analysis of Human CD14 binding using Mouse anti CD14 antibody, clone MEM-18 as a capture reagent and biotinylated Mouse anti Human CD14 antibody, clone Tük4 as a detection reagent with purified CD14 as antigen for generation of the standard curve. Detection is by HRP conjugated streptavidin. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . Serum samples diluted 1:200 and 1:400 are shown green and red respectively, while plasma samples also diluted 1:200 and 1:400 are blue and orange respectively)
WB (Western Blot) (Jurkat cells were subjected to SDS PAGE followed by western blot with AAA248016 (NOB1 Antibody) at dilution of 1:3000)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using AAA248016 (NOB1 Antibody) at dilution of 1:200)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of Jurkat cells with BPI antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
WB (Western Blot) (Western blot analysis of BPI on THP-1 cells lysates using anti-BPI antibody at 1/1, 000 dilution.)
ICC (Immunocytochemistry) (ICC staining Protein C in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Protein C in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-Protein C antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Protein C antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Protein C antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colorectal carcinoma with PCNA mouse mAb (2E1-G10-H10.1:400 diluted).showing nuclear localization.A high pressure mediated antigen retrieval step was performed in citrate buffer(pH6.0).)
ICC (Immunocytochemistry) (Immunocytochemistry staining of HeLa cells using PCNA mouse mAb (dilution 1:100).Fixed in 100% methanol for 2hr at -20?.)
IP (Immunoprecipitation) (Immunoprecipitation analysis of Hela cell lysates using PCNA mouse mAb.)
WB (Western Blot) (Western blot detection of PCNA in Hela.3T3.COS7.C6 and CHO-K1 cell lysates using PCNA mouse mAb (1:1000 diluted).Predicted band size:36KDa.Observed band size:36KDa.)
IHC (Immunohiostchemistry) (Immunohistochemistry of Clathrin heavy chain in paraffin-embedded Human colon cancer tissue using Clathrin heavy chain Rabbit mAb at dilution 1/50)
WB (Western Blot) (Western blot detection of Clathrin heavy chain in Hela,A549,HL-60,U251,U87-MG cell lysates using Clathrin heavy chain Rabbit mAb(1:1000 diluted).Predicted band size:192kDa.Observed band size:192kDa.)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant FGF2 protein (Right) or empty vector (Left) detected with FGF2 antibody)
FCM/FACS (Flow Cytometry) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant FGF2 protein (red) or empty vector (blue) stained using FGF2 antibody)
Purity: > 90% by SDS-PAGE Purification: Iron Exchange
Pricing
What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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