Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD11b antibody, clone ED7 followed by horseradish peroxidsase conjugated Goat anti Mouse IgG1 as a detection reagent. High power)
Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD11b antibody, clone ED7 followed by horseradish peroxidsase conjugated Goat anti Mouse IgG1 as a detection reagent. Medium power)
Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD11b antibody, clone ED7 followed by horseradish peroxidsase conjugated Goat anti Mouse IgG1 as a detection reagent. Low power)
FCM/FACS (Flow Cytometry) (Published customer image:Mouse anti Rabbit CD4 antibody, clone KEN-4 (AAA50241) used for the evaluation of rabbit lymphoblastoid phenotype by flow cytometry.Image caption:Lymphoblastoid cell lines (LCLs) phenotypic analysis.LCLs were propagated from peripheral blood mononuclear cells of rabbits developing MCF after infection with Delta-GE or Delta-GE-rev viruses in Iscove’s modified Dulbecco’s medium (IMDM) containing 10% FCS and supplemented with recombinant human interleukin 2 (Roche, 10 IU/mL). Cells were maintained in medium replaced every 3–4 days and analyzed by flow cytometry for IgM+ B cells, CD8+ T cells and CD4+ T cells after 3 weeks culture (A).)
FCM/FACS (Flow Cytometry) (Published researcher image:Mouse anti Rabbit CD4 antibody, clone KEN-4 (AAA50241) used to assess CD4 expression levels on rabbit mononuclear cells by flow cytometry.Image caption:Analysis of in vivo BrdU incorporation.Rabbits were treated as described in Fig. 1. PBMCs were collected at days 11, 15, 17, 20 and 24 post-inoculation, while mononuclear cells were isolated from popliteal lymph node and spleen at the time of death. Cells were labelled with anti-CD11b, IgM, CD5, CD4 and CD8 mAbs as the primary antibodies. Alexa 633-GAM was used as the secondary antibody. In vivo BrdU incorporation was revealed by immunofluorescent staining as described in Methods. After staining, cells were analysed by flow cytometry.A. Representative flow cytometry dot plots are shown for each double staining, they illustrate the data obtained at day 17 post-infection for the PBMC of rabbits MR17/1 and IR17/1. The data represent the percentages of BrdU positive cells (y-axis) calculated based on the acquisition of 10,000 cells expressing the indicated cell marker (x-axis).B. The percentage of BrdU positive cells amongst the indicated cellular subset was determined and compared between AlHV-1 infected (left column: bold lines; middle and right columns: hatched bars) and mock infected (left column: dotted lines; middle and right columns: open bars) groups (* P)
Application Data (Staining of rabbit peripheral blood lymphocytes with purified Mouse anti Rabbit CD4 antibody, clone KEN-4 (AAA50241) followed by FITC conjugated Rabbit F(ab')2 anti Mouse IgG antibody)
FITC (Fluorescein Isothiocyanate) (Staining of rabbit peripheral blood lymphocytes with FITC conjugated Mouse anti Rabbit CD4 antibody, clone KEN-4)
Application Data (Staining of thrombin activated platelets with Mouse anti Human CD62P)
Application Data (Staining of thrombin activated human platelets with Mouse anti Human CD62P:Azide Free (AAA50221))
Application Data (Staining of thrombin activated human peripheral blood platelets with Mouse anti Human CD62P: FITC)
Application Data (Staining of human peripheral blood platelets with Mouse anti Human CD62P)
Application Data (Staining of human tonsil showing capillary endothelium. Formalin fixed, paraffin processed tissue with Mouse anti Human CD62P (P-Selectin))
WB (Western Blot) (RNA pol II antibody tested by Western blot. RNA pol II detection by Western blot analysis. RNA pol II is detected in HeLa nuclear extracts using RNA pol II mouse monoclonal antibody at a 1:2,000 dilution.)
ChIP (Chromatin Immunoprecipitation) (RNA pol II antibody (mAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with chromatin from 2.3 million HL-60 cells and 20 ul of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 30 million sequence tags were mapped to identify Pol II binding. ChIP-Seq data from three specific genes is shown as an example. Pol II binding is detected at the 5´ end of the genes, throughout the gene bodies and at the 3´ end of the genes.)
Application Data (Western blot analysis of human Jurkat cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 minutes (lanes 2 & 4).The blot was probed with anti-c-Cbl (AAA71500; lanes 1 & 2) or anti-c-Cbl (Tyr-700) ( ; lanes 3 & 4).)
WB (Western Blot) (Western blot analysis of (1) 20 ng of HAT-tagged recombinant GGP1 protein and (2) 20 ng of HAT-tagged recombinant GGP1 protein using 1 μg of HAT-tag antibody to immunoprecipitate and 1 μg/ml anti-GGP1 antibody to detect.)
WB (Western Blot) (HAT-tag antibody (9A2F8)1 Western blot analysis of (1) 200 ng (2) 100 ng, (3) 50 ng and (4) 25 ng of a HAT-tag containing recombinant GGP1 protein with HAT-tag antibody at 0.5 ug/mL.)
FCM/FACS (Flow Cytometry) (Flow Cytometry analysis using Mouse Anti-N-terminal Arginylation Monoclonal Antibody, Clone 4A9 . Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-N-terminal Arginylation Monoclonal Antibody at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Heat stressed cells were subject to heat shock at 42 degree C for 2 hours.)
WB (Western Blot) (Western Blot analysis of N-terminal Arginine-BSA showing detection of 67 kDa N-terminal Arginylation protein using Mouse Anti-N-terminal Arginylation Monoclonal Antibody, Clone 4A9 . Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: RDHKH-BSA. Lane 4: REHKH-BSA. Lane 5: HKH-BSA. Lane 6: HKERD-BSA. Lane 7: HKRRE-BSA. Load: 0.5 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-N-terminal Arginylation Monoclonal Antibody at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 67 kDa. Other Band(s): 250kDa, 150kDa, 75kDa REHKH-BSA.)
IF (Immunofluorescence) (ICC/IF analysis of Clusterin in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human Clusterin antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
IHC (Immunohiostchemistry) (Paraffin embedded sections of human renal cell carcinoma tissue were incubated with anti-human Clusterin (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB))
WB (Western Blot) (Cell lysates of U87MG (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Clusterin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Nav1.7 Sodium Channel Monoclonal Antibody, Clone S68-6. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Nav1.7 Sodium Channel Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.)
IHC (Immunohistochemisry) (Immunohistochemistry analysis using Mouse Anti-Nav1.7 Sodium Channel Monoclonal Antibody, Clone S68-6. Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Nav1.7 Sodium Channel Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.)
WB (Western Blot) (Western Blot analysis of hamster CHO cells showing detection of Nav1.7 Sodium Channel protein using Mouse Anti-Nav1.7 Sodium Channel Monoclonal Antibody, Clone S68-6. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Nav1.7 Sodium Channel Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
WB (Western Blot) (fetal human brain tissue were subjected to SDS PAGE followed by western blot with AAA102713 (BRD8 Antibody) at dilution of 1:1000)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using AAA102713 (BRD8 Antibody) at dilution of 1:200)
IF (Immunofluorescence) (ICC/IF analysis of SHMT1 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human SHMT1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot) (The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SHMT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: A549 cell lysate Lane 2.: HeLa cell lysate Lane 3.: NIH-3T3 cell lysate Lane 4.: LnCaP cell lysate)
IF (Immunofluorescence) (ICC/IF analysis of CDC37 in Balb/3T3 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CDC37 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot) (The cell line and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CDC37 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Jurkat cell lysate Lane 2. : A431 cell lysate Lane 3. : K562 cell lysate Lane 4. : LnCap cell lysate Lane 5. : SW480 cell lysate Lane 6. : HeLa cell lysate Lane 7. : MCF7 cell lysate Lane 8. : 293T cell lysate)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant SulT1C2 protein (Right) or empty vector (Left) detected with SulT1C2 antibody)
FCM/FACS (Flow Cytometry) (Flow Cytometric analysis of Hela cells using SulT1C2 antibody (Red), compared to a nonspecific negative control antibody (Blue).)
WB (Western Blot) (Western Blot analysis using SERPINB2 antibodyWestern Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant SERPINB2 protein (Right) or empty vector (Left) detected with SERPINB2 antibody)
FCM/FACS (Flow Cytometry) (Flow Cytometric analysis using SERPINB2 antibodyFlow Cytometric analysis of HEK293T cells transfected with either recombinant SERPINB2 protein (red) or empty vector (blue) stained using SERPINB2 antibody)
IF (Immunofluorescence) (Immunofluorescence analysis of Hela cells using SCGB2A2 antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded mammary cancer tissues using SCGB2A2 antibody with DAB staining.)
WB (Western Blot) (Western Blot showing SCGB2A2 antibody used against human SCGB2A2 (AA: 2-93) recombinant protein. (Expected MW is 35.8 kDa))
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant SSX5 protein using SSX5 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant SSX5 protein (Right) or empty vector (Left) detected with SSX5 antibody)
FCM/FACS (Flow Cytometry) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant SSX5 protein (red) or empty vector (blue) stained using SSX5 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant PDIA4 protein (Right) or empty vector (Left) detected with PDIA4 antibody)
IHC (Immunohistochemistry) (Immunohistochemical analysis of PDIA4 protein in paraffin embedded Human Kidney tissue using PDIA4 antibody)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of ALG2 protein in paraffin embedded Human endometrium tissue using ALG2 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ALG2 protein (Right) or empty vector (Left) detected with ALG2 antibody)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-Nudel antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-Nudel antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Nudel antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Nudel on rat brain tissue lysate using anti-Nudel antibody at 1/2, 000 dilution.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Thrombomodulin antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Thrombomodulin antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Thrombomodulin on A431 cell using anti-Thrombomodulin antibody at 1/500 dilution.)
ICC (Immunocytochemistry) (ICC staining Musashi 1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Musashi 1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Musashi 1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Ki67 antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Ki67 antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.)
WB (Western Blot) (Western blot analysis of Ki67 on HepG2 cell lysates using anti-Ki67 antibody at 1/1, 000 dilution.)
ICC (Immunocytochemistry) (ICC staining RPS3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining RPS3 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-RPS3 antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-RPS3 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of RPS3 on PC12 cell lysates using anti-RPS3 antibody at 1/500 dilution.)
IHC (Immunohiostchemistry) (Immunohistochemistry of TXNRD2 in paraffin-embedded Human Cholangiocarcinoma using TXNRD2 Rabbit mAb at dilution 1/20)
WB (Western Blot) (Western blot detection of TXNRD2 in K562,Hela cell lysates using TXNRD2 Rabbit mAb(1:1000 diluted).Predicted band size:57kDa.Observed band size:57kDa.)
IHC (Immunohiostchemistry) (Immunohistochemical of HDAC9 in Human tonsil tissue using HDAC9 antibody at dilution 1/20)
WB (Western Blot) (Western blot detection of HDAC9 in A549,HL-60 cell lysates using HDAC9 Rabbit mAb(1:1000 diluted).Predicted band size:111kDa.Observed band size:150kDa.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of Hela cells using ASK 1 Monoclonal Antibody (green) and negative control (purple).)
IF (Immunofluorescence) (Immunofluorescence analysis of Hela cells using ASK 1 Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded breast cancer tissues (left) and prostate tissues (right) with DAB staining using ASK 1 Monoclonal Antibody.)
WB (Western Blot) (Western Blot analysis using ASK 1 Monoclonal Antibody against recombinant protein.)
Application Data (Pietilä. E.A.. Gonzalez-Molina. J.. Moyano-Galceran. L. et al. Co-evolution of matrisome and adaptive adhesion dynamics drives ovarian cancer chemoresistance. Nat Commun 12. 3904 (2021). h)
Application Data (Li. J.. Li. Y.. Zhou. X. et al. Upregulation of IL-15 in the placenta alters trophoblasts behavior contributing to gestational diabetes mellitus. Cell Biosci 11. 33 (2021). )
Application Data (Jukonen. J.. Moyano-Galceran. L.. Höpfner. K. et al. Aggressive and recurrent ovarian cancers upregulate ephrinA5. a non-canonical effector of EphA2 signaling duality. Sci Rep11. 8856 (2021).)
WB (Western Blot) (Western blot analysis of 1) Hela. 2) Mouse Kidney. 3) Mouse Brain. diluted at 1:2000.)
Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Purified by Protein-A Affinity Chromatography
Pricing
What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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