Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
IHC (Immunohistochemistry) (Formaldehyde-fixed paraffin-embedded (FFPE) Tissue Slides. Human heart ventricle following immunostaining with cMHC 3-48 and irrelevant Neuro monoClonal antibody at low microscopical magnification. A: cMHC 3-48-labeled cardiomyocytes. FFPE section of human heart ventricle was submitted to HIAR condition with citrate B buffer prior to immunostaining. Immunostaining was revealed following incubation with biotinylated goat anti-mouse IgG, 20 minutes at 20C and horse radish peroxidase-diaminobemzidine enzymatic reaction causing brown precipitate to appear atthe sites of primary antibody binding. B: Control immunostaining performed following HIAR condition on the same microscopical slide with Neuro monoclonal antibody showing no immunostaining but presence of light background, emphasizing specificity of immunostaining in (A). Magnification x30 C: Fragment of the same slide as in (A) with heart ventricle cardiomyocytes immunostained (brown). Magnification x580)
Cardiac Myosin Heavy Chain Mouse Monoclonal (3-48_G5C7)
Gene Names
MYH7; CMH1; MPD1; SPMD; SPMM; CMD1S; MYHCB
Reactivity
Human, Rat, Mouse Predicted: Canine, Bovine and Rabbit
Applications
Immunohistochemistry, Immunofluorescence, Western Blot
Pricing
Application Data (Sandwich ELISA: In combination with anti-human IL-17A clone 4C1 coated ELISA plate, the anti-human IL-17A antibody clone 3H11, when conjugated with Biotin, can be used for quantitative measurement of human IL-17A in sandwich ELISA. The dynamic range of the standard curve is 1000pg/ml to 15.6pg/ml.)
Application Data (HRS cells of the classical Hodgkin Lymphoma showing cytoplasmic expression of the EBV LMP-1 protein. Formalin fixed, paraffin embedded human tissue (4 Um section) stained with anti - EBV LMP-1 (AAA78994) monospecific clonal antibody according to related DB Biotech datasheet.)
FCM/FACS (Flow Cytometry) (Flow-cytometry using anti-human IL11RA antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL11RA antibody monoclonal antibody (Catalog # RHG84901 ,Green Histogram) at a concentration of 5 ?ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # PHB96441) and cells analysed on a NovoCyte Flow Cytometer.)
FCM/FACS (Flow Cytometry) (Flow-cytometry using anti-human IL11RA antibody.IL11RA Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL11RA antibody monoclonal antibody (Catalog # RHG84901 ,Green Histogram) at a concentration of 5 ?ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # PHB96441) and cells analysed on a NovoCyte Flow Cytometer.)
IHC (Immunohiostchemistry) (Immunohistochemistry of GSK3 beta in paraffin-embedded Human Cholangiocarcinoma using GSK3 beta Rabbit mAb at dilution 1:20)
WB (Western Blot) (Western blot detection of GSK3 beta in Jurkat,C6,CHO-K1,Hela cell lysates using GSK3 beta Rabbit mAb(1:1000 diluted).Predicted band size:47kDa.Observed band size:47kDa.)
IF (Immunofluorescence) (Immunofluorescent analysis of MCF-7 cells, using Akt(pan)1/2/3 Antibody.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human testis, using Akt(pan)1/2/3 Antibody.)
WB (Western Blot) (Western blot analysis of Akt(pan)1/2/3 expression in (1) A549 cell lysate; (2) Mouse brain lysate; (3) Rat brain lysate; (4) Rat heart lysate; (5) Rat kidney lysate.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of MCF-7 cells with Tropomyosin 1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Tropomyosin 1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human heart tissue using anti-Tropomyosin 1 antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded rat smooth muscle tissue using anti-Tropomyosin 1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Tropomyosin 1 on rat heart tissue using anti-Tropomyosin 1 antibody at 1/1, 000 dilution.)
ICC (Immunocytochemistry) (ICC staining JunD in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining JunD in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of Hela cells with CDC42 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-CDC42 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-CDC42 antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-CDC42 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of CDC42 on different lysates using anti-CDC42 antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat Lane 3: HepG2)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-LAMP1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of LAMP1 on different lysates using anti-LAMP1 antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat)
ICC (Immunocytochemistry) (ICC staining MAP1LC3A in PMVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining MAP1LC3A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-MAP1LC3A antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of MAP1LC3A on mouse brain lysates using anti-MAP1LC3A antibody at 1/1, 000 dilution.)
ICC (Immunocytochemistry) (ICC staining PSA in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-PSA antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PD1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of over-expressed PD1 (whole extracellular domain) on 293T cell lysate using anti-PD1 antibody at 1/500 dilution.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-CD63 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of CD63 on different lysates using anti-CD63 antibody at 1/1, 000 dilution. Positive control: Lane 1: HL60 Lane 2: THP-1 Lane 3: NIH/3T3)
WB (Western Blot) (Western blot analysis of CD63 on different lysates using anti-CD63 antibody at 1/1, 000 dilution. Positive control: Lane 1: HL60 Lane 2: THP-1 Lane 3: NIH/3T3)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of 293T cells with RPA70 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining RPA70 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining RPA70 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining RPA70 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
WB (Western Blot) (Western blot analysis of RPA70 on Siha cell lysates using anti-RPA70 antibody at 1/500 dilution.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of Jurkat cells with THAP1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).)
ICC (Immunocytochemistry) (ICC staining THAP1 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
WB (Western Blot) (Western blot analysis of THAP1 on HEK293 (1) and THAP1-hIgGFc transfected HEK293 (2) cell lysate using anti-THAP1 antibody at 1/1, 000 dilution.)
WB (Western Blot) (Western blot analysis of THAP1 on human THAP1 recombinant protein using anti-THAP1 antibody at 1/1, 000 dilution.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of Hela cells with Interferon gamma antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-Interferon gamma antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Interferon gamma antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Interferon gamma antibody. Counter stained with hematoxylin.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of Jurkat cells with Ctip2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
ICC (Immunocytochemistry) (ICC staining Ctip2 in LO2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Ctip2 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Ctip2 on THP-1 cells lysates using anti-Ctip2 antibody at 1/1, 000 dilution.)
IHC (Immunohiostchemistry) (Immunohistochemistry of MMP14 in paraffin-embedded Human breast cancer tissue using MMP14 Rabbit mAb at dilution 1/100)
WB (Western Blot) (Western blot detection of MMP14 in CHO-K1 cell lysates using MMP14 Rabbit mAb(1:1000 diluted).Predicted band size:66kDa.Observed band size:66kDa.)
IHC (Immunohiostchemistry) (Immunohistochemistry of BNIP3L in paraffin-embedded Human lung cancer tissue using BNIP3L Rabbit mAb at dilution 1/50)
WB (Western Blot) (Western blot detection of BNIP3L in Jurkat cell lysates using BNIP3L Rabbit mAb(1:500 diluted).Predicted band size:24kDa.Observed band size:38kDa.)
IHC (Immunohistochemistry) (IHC analysis of formalin-fixed, paraffin-embedded human placenta stained using ALPP/4109 at 2ug/ml in PBS for 30min RT. Inset: PBS used instead of the primary antibody as the negative control.)
Application Data (Analysis of Protein Array containing more than 19,000 full-length human proteins using Desmoglein-3 Mouse Monoclonal Antibody (DSG3/2840) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
SDS-PAGE (SDS-PAGE Analysis of Purified Desmoglein-3 Mouse Monoclonal Antibody (DSG3/2840). Confirmation of Purity and Integrity of Antibody)
IHC (Immunohiostchemistry) (Formalin-fixed, paraffin-embedded human Esophageal Carcinoma stained with Desmoglein-3 Mouse Monoclonal Antibody (DSG3/2840).)
IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Esophageal Carcinoma stained with Desmoglein-3 Mouse Monoclonal Antibody (DSG3/2840).)
IHC (Immunohiostchemistry) (Immunohistochemistry of SP1 in paraffin-embedded Human colon cancer tissue using SP1 Rabbit mAb at dilution 1/50)
WB (Western Blot) (Western blot detection of SP1 in K562,3T3,Hela cell lysates using SP1 Rabbit mAb(1:1000 diluted).Predicted band size:81kDa.Observed band size:90kDa.)
IF (Immunofluorescence) (Immunofluorescence of NFkB p100(green) in Hela cells using NFkB p100 Rabbit mAb at dilution 1/200, and DAPI(blue))
WB (Western Blot) (Western blot detection of NFkB p100/NFKB2 in C6,3T3,Hela cell lysates using NFkB p100/NFKB2 Rabbit mAb(1:1000 diluted).Predicted band size:97kDa.Observed band size:100kDa.)
IHC (Immunohiostchemistry) (IHC image diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)
WB (Western Blot) (Western BlotPositive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, L02 whole cell lysateAll lanes: CYP1A2 antibody at 1:1000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 59 kDaObserved band size: 59 kDa)
Western Blot, Immunohistochemistry, Immunocytochemistry
Purity
Affinity Chromatography
Pricing
FCM/FACS (Flow Cytometry) (Fig-1: Cell Surface FLOW analysis of PD-L1- PE in PHA treated human PBMC using 1 ug of PE conjugated PD-L1 antibody (Clone: ABM4E54). Green represents isotype control; red represents anti-PD-L1-PE (10-7562-PE) antibody.)
Monoclonal Antibody to Human Chorionic Gonadotropin- Beta (hCG-beta)
Applications
Western Blot
Purity
Raw ascites made at MBS is applied to a Protein A affinity chromatography column. The antibody is eluted from the column at pH 5.0. It is then buffer exchanged to PBS, pH 7.6 with 0.1% NaN3. Purity: determined by Size Exclusion Chromatography
Pricing
What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
Submit a Question
Please complete the form below and a representative will contact you as soon as possible.
Request more Information
Please complete the form below and a representative will contact you as soon as possible.
Request a Manual
Please complete the form below and a representative will contact you as soon as possible.
Request a Quote
Please complete the form below and a representative will contact you as soon as possible.