Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
WB (Western Blot) (Western Blot analysis of over-expressed Myc-tagged protein in 293T cell lysate using Myc-Tag (19C2) Mouse mAb. The antibody dilutions are 1:5000 (lane1) and 1:10000 (lane 2). Each lane was loaded with 10ug of cell lysate.)
IP (Immunoprecipitation) (IP of extracts from 293T cells transfected (lane 1) with a Myc-tagged protein and a mock transfection (lane 2, using the same protein without the Myc tag), using Myc-Tag (19C2) Mouse mAb and probed on Western blot using the same antibody. Dilution: 1:200 (1), 1:500 (2), 1:200 (3).)
IF (Immunofluorescence) (IF analysis of 293T cells transfected (left) with a Myc-tagged protein and a mock transfection (right, using the same protein without the Myc tag), using Myc-Tag (19C2) Mouse mAb at a 1:2000 dilution.)
WB (Western Blot) (Western blot analysis of over-expressed Myc-tagged protein in 293T cell lysate, using Myc-tag (19C2) Mouse mAb. The antibody dilutions are 1:5000 (lane 1) and 1:10000 (lane 2). Each lane was loaded with 10 ug of cell lysate.)
IHC (Immunohiostchemistry) (Immunohistochemistry of SMARCA2/BRM in paraffin-embedded Human lung cancer tissue using SMARCA2/BRM Rabbit mAb at dilution 1:50)
WB (Western Blot) (Western blot detection of SMARCA2/BRM in A549,HL-60,U2OS cell lysates using SMARCA2/BRM Rabbit mAb(1:1000 diluted).Predicted band size:181kDa.Observed band size:190kDa.)
IF (Immunofluorescence) (Immunofluorescence of MCM2(green) in Hela cells using MCM2 Rabbit mAb at dilution 1:50, and DAPI(blue))
WB (Western Blot) (Western blot detection of MCM2 in K562,C6,3T3,Hela cell lysates using MCM2 Rabbit mAb(1:1000 diluted).Predicted band size:102kDa.Observed band size:125kDa.)
ICC (Immunocytochemistry) (ICC staining Phospho-AMPK alpha 2 (S345) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Phospho-AMPK alpha 2 (S345) in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Phospho-AMPK alpha 2 (S345) antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Phospho-AMPK alpha 2 (S345) antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-NACC1 antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human mammary cancer tissue using anti-NACC1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of NACC1 on HEK293 (1) and NACC1-hIgGFc transfected HEK293 (2) cell lysate using anti-NACC1 antibody at 1/1, 000 dilution.)
ICC (Immunocytochemistry) (ICC staining KLHL22 (green) and actin filaments (red) in U251 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
WB (Western Blot) (Western blot analysis of KLHL22 on mouse brain tissue lysate using anti-KLHL22 antibody at 1/1, 000 dilution.)
ICC (Immunocytochemistry) (ICC staining Smad1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Smad1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Smad1 in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Smad1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining CCR7 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining CCR7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CCR7 antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CCR7 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of CCR7 on different cell lysates using anti-CCR7 antibody at 1/1, 000 dilution. Positive control: Lane 1: MCF-7 Lane 2: PC-12 Lane 3: Daudi Lane 4: Jurkat)
IF (Immunofluorescence) (Immunofluorescent analysis of HeLa cells, using FGFR1 Antibody)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Rat pancreas, using the Antibody at 1:50 dilution.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human thymoma, using the Antibody at 1:50 dilution.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human pituitary tumor, using the Antibody at 1:50 dilution.)
WB (Western Blot) (Western blot analysis of FGFR1 expression in SH-SY5Y cell lysate.)
ICC (Immunocytochemistry) (ICC staining FGFR1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining FGFR1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
WB (Western Blot) (Western blot analysis of FGFR1 on 293 cells lysates using anti-FGFR1 antibody at 1/1, 000 dilution.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Von Willebrand Factor antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Von Willebrand Factor on human lung lysates using anti-Von Willebrand Factor antibody at 1/1, 000 dilution.)
ICC (Immunocytochemistry) (ICC staining AKR7A2 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
WB (Western Blot) (Western blot analysis of AKR7A2 on human spermatozoa lysate using anti-AKR7A2 antibody at 1/1, 000 dilution. )
IHC (Immunohistochemisry) (Immunohistochemistry of Anterior Gradient 2 in paraffin-embedded colon using Anterior Gradient 2 Rabbit mAb at dilution 1/100)
IF (Immunofluorescence) (Immunofluorescence of Anterior Gradient 2 (green) in MCF-7 using Anterior Gradient 2 antibody at dilution 1/2, and DAPI(blue))
WB (Western Blot) (Western blot detection of Anterior Gradient 2 in K562,C6 cell lysates using Anterior Gradient 2 Rabbit mAb(1:1000 diluted).Predicted band size:20kDa.Observed band size:18kDa.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Gephyrin antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Gephyrin antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.)
WB (Western Blot) (Western blot analysis of Gephyrin on SK-Br-3 cell (1) and rat kidney tissue (2) lysate using anti-Gephyrin antibody at 1/500 dilution.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-FHL2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human fetal heart tissue using anti-FHL2 antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-FHL2 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of FHL2 on different tissue lysates using anti-FHL2 antibody at 1/1, 000 dilution. Positive control: Lane 1: Rat heart Lane 2: Mouse heart)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of Hela cells with Lin-28 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
WB (Western Blot) (Western blot analysis of Lin-28 on NCCIT cells lysates using anti-Lin-28 antibody at 1/500 dilution.)
WB (Western Blot) (Western blot analysis of Phospho-Nucleolin (T84) expression in (1) K562 cell lysate; (2) K562 cell treated with Calyculin A lysate.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded Human colon cancer using AlkylDHAP synthase antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.)
WB (Western Blot) (Western blot analysis of AlkylDHAP synthase in K562 lysates using AGPS antibody.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human Skin Carcinoma Tissue using Collagen I Mouse mAb diluted at 1:200.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma Tissue using Collagen I Mouse mAb diluted at 1:200.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma Tissue using Collagen II Mouse mAb diluted at 1:200)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma Tissue using Collagen II Mouse mAb diluted at 1:200)
IF (Immunofluorescence) (IF analysis of 293 cells transfected with a Myc-tag protein,1:2000 dilution (blue DAPI, red anti-Myc))
IP (Immunoprecipitation) (IP antibody use: 3ug DYKDDDDK Mouse IgG1 per ml Lysate, WB 1: 50001)Untransfected 293 cell lysate2)Transfected 293 cell lysate with Myc-Tag fusion protein3)IP (transfected 293+ normal Mouse IgG+Protein G agarose)4)IP (transfected 293+anti-C-Myc m)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of Hela cells using BID Monoclonal Antibody (green) and negative control (purple))
IF (Immunofluorescence) (Immunofluorescence analysis of Hela cells using BID Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded prostate tissues (left) and tonsil tissues (right) with DAB staining using BID Monoclonal Antibody.)
WB (Western Blot) (Western Blot analysis using BID Monoclonal Antibody against HeLa (1). A431 (2). Jurkat (3). A549 (4). HepG2 (5). and HEK293 (6) cell lysate.)
The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
IHC (Immunohiostchemistry) (Immunohistochemical analysis of GPI protein in paraffin embedded Human pancreas tissue using GPI antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant GPI protein (Right) or empty vector (Left) detected with GPI antibody)
IF (Immunofluorescence) (ICC/IF analysis of GARS in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GARS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot) (The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GARS (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: 293T cell lysate Lane 3.: Jurkat cell lysate Lane 4.: U87-MG cell lysate Lane 5.: A431 cell lysate)
IF (Immunofluorescence) (ICC/IF analysis of COX5A in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human COX5A antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot) (The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human COX5A antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : A431 cell lysate Lane 2. : HeLa cell lysate)
IF (Immunofluorescence) (Immunofluorescence of human HeLa cells stained with monoclonal anti-human KCDT15 antibody (1:500) with Texas Red (Red). Nucleus was stained by Hoechst 33342 (Blue).)
WB (Western Blot) (Cell lysates of HeLa (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human KCTD15 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KCTD15 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : 293T cell lysate Lane 2 : KCTD15 Transfected 293T cell lysate)
WB (Western Blot) (The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PBEF/Visfatin antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: SW480 cell lysate)
ICC (Immunocytochemistry) (ICC/IF analysis of PBEF/Visfatin in HeLa cells. The cell was stained (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
Immunofluorescence, Immunocytochemistry, Western Blot
Purity
By protein-A affinity chromatography
Pricing
WB (Western Blot) (Western blot analysis of ADAM17 expression in Hela cell lysate (AAA46966).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADAM17 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ADAM17)
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Flow Cytometry
Purity
Affinity-chromatography
Pricing
What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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