Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
FCM/FACS (Flow Cytometry) (Intracellular flow analysis of MyD88 in Jurkat using 0.5 ug/10^6 cells of MyD88 antibody (Clone: ABM2H20). Green represents isotype control; red represents anti-MyD88 antibody. Goat anti-mouse PE conjugate was used as secondary antibody.)
WB (Western Blot) (Expression analysis of MyD88. Anti-MyD88 antibody (Clone: ABM2H20) was tested at 0.1 ug/ml on h Kidney and Jurkat cells.)
WB (Western Blot) (Western blot analysis of His-tag on standard ladder containing fIVe different HIs-lagged proteins; untransfected control (1), HEK293 cells transfected with His-tagged protein vector (2).)
IF (Immunofluorescence) (Immunofluorescence analysis of HEK293 cells transfected with His-tagged fusion protein, stained with anti-His (red) and DAPI (blue). left: untransfected. Right; transfected.)
IHC (Immunohiostchemistry) (Immunohistochemical staining patterns of formalin fixed and paraffin embedded human kidney tissue (4 ?m sections) with Anti – Napsin A monospecific antibody, according to datasheet, using Leica Bond-Max. The kidney tissues show a moderate to strong, granular cytoplasmic staining reaction of the majority of the epithelial cells of the proximal tubules.)
IHC (Immunohistochemistry) (Immunohistochemical staining patterns of formalin fixed and paraffin embedded human kidney tissue (4 ?m sections) with Anti – Napsin A monospecific antibody, according to datasheet, using Ventana BenchMark. The kidney tissues show a moderate to strong, granular cytoplasmic staining reaction of the majority of the epithelial cells of the proximal tubules.)
FCM/FACS (Flow Cytometry) (Human peripheral blood lymphocytes are stained with Anti-Human CD18 Monoclonal Antibody(Alexa Fluor 647 Conjuaged)(filled gray histogram). Unstained lymphocytes (empty black histogram) are used as control.)
FCM/FACS (Flow Cytometry) (Figure 5. Flow Cytometry analysis of HL-60 cells using anti-PARP antibody (AAA125899).Overlay histogram showing HL-60 cells stained with AAA125899 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- PARP Antibody (AAA125899, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
IF (Immunofluorescence) (Figure 4. IF analysis of PARP using anti- PARP antibody (AAA125899).PARP was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL mouse anti- PARP Antibody (AAA125899) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
IHC (Immunohistochemisry) (Figure 3. IHC analysis of PARP using anti-PARP antibody (AAA125899).PARP was detected in paraffin-embedded section of human ovarian serous adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA uffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-PARP Antibody (AAA125899) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
IHC (Immunohiostchemistry) (Figure 2. IHC analysis of PARP using anti-PARP antibody (AAA125899).PARP was detected in paraffin-embedded section of human gallbladder adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-PARP Antibody (AAA125899) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
WB (Western Blot) (Figure 1. Western blot analysis of PARP using anti-PARP antibody (AAA125899).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human MCF-7 whole cell lysatesLane 2: human HELA whole cell lysatesLane 3: human DAUDI whole cell lysatesLane 4: human HEK293 whole cell lysatesLane 5: rat testis tissue lysatesLane 6: mouse testis tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- PARP antigen affinity purified monoclonal antibody (Catalog # AAA125899) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PARP at approximately 113KD. The expected band size for PARP is at 113KD.)
WB (Western Blot) (Chd5 antibody (mAb) (Clone 5A10) tested by Western blot. Chd5 antibody detection by Western blot. The analysis was performed using 40 ug of HeLa nuclear extract and Chd5 antibody at a 2 ug/ml dilution.)
IF (Immunofluorescence) (Chd5 antibody (mAb) (Clone 5A10) tested by immunofluorescence. Left: HeLa cells. Right: E12.5 mouse gut. Both stained with Chd5 antibody.)
>90%, Purified pure mouse monoclonal antibody which has been purified from ascites fluid or culture medium by protein A chromatography or sequential differential precipitations.
IF (Immunofluorescence) (ImmunofluorescencestainingofRAW264.7cellswithAnti-NKG2DCX-5 ImmunofluorescenceanalysisofparaformaldehydefixedRAW264.7cellsstainedwiththechimericrabbitIgGversionofCX-5(1:100dilution)for1hfollowedbyAlexaFluor488secondaryantibody(1:1000dilution),showingmembraneandcytoplasmicstaining.ThenuclearstainisDAPI(blue).Panelsshowfromleft-right,top-bottomAb02365,DAPI,mergedchannelsandanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)
IF (Immunofluorescence) (ImmunofluorescencestainingofMDA-MB-231cellswithanti-PD-L1antibodyB7HC0013(AAA72536). ImmunofluorescenceanalysisofparaformaldehydefixedMDA-MB-231cellsstainedwiththechimericrabbitIgGversionofB7HC0013(AAA72536)(1:100dilution)for1hfollowedbyAlexaFluor488secondaryantibody(1:1000dilution),showingmembraneandcytoplasmicstaining.ThenuclearstainisDAPI(blue).Panelsshow,fromleft-right,top-bottom,AAA72536,DAPI,mergedchannelsandanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of BUB1B protein in paraffin embedded Adenocarcinoma of Human colon tissue using BUB1B antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant BUB1B protein (Right) or empty vector (Left) detected with BUB1B antibody)
FCM/FACS (Flow Cytometry) (HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-TFAP2A mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. 959C2a-1])
WB (Western Blot) (Detection of TFAP2A by Western blot.Samples: Whole cell lysate from human HEK293 (H, 50 ug) and mouse NIH3T3 (M, 50 ug) cells. [Lot No. 959C2a-1]Predicted molecular weight: 48 kDa)
Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control (Western blot analysis of immunized recombinant protein, using anti-TFAP2A monoclonal antibody.)
WB (Western Blot) (Western Blot using anti-WDR5 antibody RAB-C223 (AAA72208). Nuclear lysate of MCF7 cells (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of RAB-C223 at 1ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
FCM/FACS (Flow Cytometry) (Flow cytometry using the Anti-WDR5 antibody RAB-C223 (AAA72208). Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody or the rabbit IgG version of RAB-C223 at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
PKM2 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
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What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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