Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
FCM/FACS (Flow Cytometry) (Figure 1. ELISA plate pre-coated by 1 ug/ml (100 ul/well) Human BTLA , His tagged protein PME100524 can bind Anti-BTLA Neutralizing antibody (AAA47500) in a linear range of 1-100 ng/ml.)
ELISA (Figure 1. ELISA plate pre-coated by 1 ug/ml (100 ul/well) Human BTLA , His tagged protein PME100524 can bind Anti-BTLA Neutralizing antibody (AAA47500) in a linear range of 1-100 ng/ml.)
ICC (Immunocytochemistry) (Immunocytochemical labeling of CD244 in aldehyde fixed and NP-40 permeabilized PMA-differentiated human THP-1 cells. The cells were labeled with mouse monoclonal anti-CD244/2B4/SLAMF4 (CM0531). The antibody was detected using goat anti-mouse DyLight 594.)
WB (Western Blot) (Western blot of native (lane 1 and 3) and denatured (lane 2 and 4) lysates of human THP-1 monocytes. The blot was probed with mouse monoclonals anti-CD244/2B4/SLAMF4 (CM0531) (lanes 1 and 2) or anti-CD244/2B4/SLAMF4 (CM0851). Both antibodies were used at 1:250.)
WB (Western Blot) (Western Blot analysis of Rat cell lysates showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone D7Alpha. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone D7alpha. Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:100000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. Inflammatory cells.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone D7alpha. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:100000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Magnification: 40x.)
WB (Western Blot) (Western Blot analysis of Human Cell line lysates showing detection of GABA A Receptor protein using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone S95-35. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone S95-35. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dermal Cells.)
WB (Western Blot) (Western Blot analysis of Mouse Cortex and Cerebellum showing detection of 184 kDa MDC1 protein using Mouse Anti-MDC1 Monoclonal Antibody, Clone P2B11. Lane 1: MW ladder. Lane 2: Mouse Cortex. Lane 3: Mouse Cerebellum. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-MDC1 Monoclonal Antibody at 1:1000 for 2 hours RT. Secondary Antibody: Goat Anti-Mouse at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 184 kDa.)
WB (Western Blot) (Western Blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of 184 kDa MDC1 protein using Mouse Anti-MDC1 Monoclonal Antibody, Clone P2B11. Lane 1: MW ladder. Lane 2: 293Trap cell lysates. Load: 30 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-MDC1 Monoclonal Antibody at 1:1000 for 2 hours RT. Secondary Antibody: Goat Anti-Mouse HRP: IgG at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 184 kDa.)
IHC (Immunohistochemisry) (Immunohistochemistry analysis using Mouse Anti-Slo2.2 Potassium Channel Monoclonal Antibody, Clone S3-26. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Slo2.2 Potassium Channel Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Suprabasal epidermal staining. Hair follicles negative.)
WB (Western Blot) (Western Blot analysis of Rat brain membrane lysate showing detection of Slo2.2 Potassium Channel protein using Mouse Anti-Slo2.2 Potassium Channel Monoclonal Antibody, Clone S3-26. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Slo2.2 Potassium Channel Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Slo2.2 Potassium Channel Monoclonal Antibody, Clone S3-26. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20 degree C. Primary Antibody: Mouse Anti-Slo2.2 Potassium Channel Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dotty staining around nucleus and some in cytoplasm.)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant FAHD2A protein using FAHD2A antibody)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of FAHD2A protein in paraffin embedded Human liver tissue using FAHD2A antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant FAHD2A protein (Right) or empty vector (Left) detected with FAHD2A antibody)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of HMOX2 protein in paraffin embedded Human endometrium tissue using HMOX2 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant HMOX2 protein (Right) or empty vector (Left) detected with HMOX2 antibody)
Application Data (Image: Blots of recombinant human Aldolase A, B and C as indicated showing binding to all three gene products. Lane labelled S show molecular weight standards, while lanes A, B and C contain recombinant full length His-tagged human Aldolase A, B and C respectively. The epitope is within the core of the three Aldolase molecules which are less conserved than the N and C-termini where our Aldolase C specific antibodies bind. The specificity of our other two Aldolase antibodies is shown in the indicated parts of the image. One antibody binds to the N-terminal peptide and one to the C-terminal peptide)
Application Data (IHC Image: View of mixed neuron/glial cultures stained with (red) and our chicken antibody to GFAP antibody (green). antibody reveals strong cytoplasmic staining in astrocytes. Blue is a DNA stain. Aldolase stains the astrocytes cell body and processes, whereas GFAP labels the intermediate filament of the cytoskeleton in subset of astrocytes.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of FAM84B protein in paraffin embedded Adenocarcinoma of Human ovary tissue using FAM84B antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant FAM84B protein (Right) or empty vector (Left) detected with FAM84B antibody)
FCM/FACS (Flow Cytometry) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant FAM84B protein (red) or empty vector (blue) stained using FAM84B antibody)
WB (Western Blot) (Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Hexanoyl-Lysine adduct protein using Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody, Clone 5D9. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysate. Lane 3: H2O2 treated HeLa cell lysate. Load: 12 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT.)
FCM/FACS (Flow Cytometry) (Flow Cytometry analysis using Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody, Clone 5D9. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Cells were subject to oxidative stress by treating with 250 uM H2O2 for 24 hours.)
WB (Western Blot) (Western Blot analysis of Hexanoyl Lysine-BSA Conjugate showing detection of 67 kDa Hexanoyl-Lysine adduct protein using Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody, Clone 5D9. Lane 1: Molecular Weight Ladder (MW). Lane 2: Hexanoyl Lysine-BSA. Lane 3: BSA. Load: 0.5 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.)
ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody, Clone 5D9. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Hexanoyl-Lysine adduct Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
IHC (Immunohiostchemistry) (Figure 2: Formalin-fixed, paraffin-embedded rat spleen stained with mouse anti-rat CD43 Monoclonal Antibody (Wen3). 5ug antibody was used.)
FCM/FACS (Flow Cytometry) (Figure 1: Cell surface staining of rat splenocytes. Green represents isotype control, red represents anti-rat CD43 antibody (10-4293). 0.2 ug antibody was used. Goat anti-rat FITC was used as secondary antibody.)
Application Data (Fig 2: Cell surface staining of HepG2 cells. 2ug of antibody was used. Green Isotype control. Red Insulin Receptor antibody.)
Application Data (Fig 1: Cell surface staining of PBMC, lymphocytes gated. 1ug of antibody was used. Green Isotype control. Red Insulin Receptor antibody.)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant SLC2A5 protein using SLC2A5 antibody)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of SLC2A5 protein in paraffin embedded Human liver tissue using SLC2A5 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant SLC2A5 protein (Right) or empty vector (Left) detected with SLC2A5 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant NECAB1 protein (Right) or empty vector (Left) detected with NECAB1 antibody)
FCM/FACS (Flow Cytometry) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant NECAB1 protein (red) or empty vector (blue) stained using NECAB1 antibody)
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoblot
Pricing
ELISA (ELISA plate was coated with purified human IgG, IgM, IgA, and IgE. Immunoglobulins were detected with serially diluted Mouse Anti-Human IgE Fc-BIOT followed by Streptavidin-HRP)
WB (Western Blot) (Western blot analysis of 15 ug cell lysates from DH5α and BL21 cells. Samples were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, probed with 5 ug/mL purified anti-Sigma 32 antibody, clone 3RH3, followed by incubation with horseradish peroxidase (HRP) labeled goat anti-mouse secondary antibody (Cat. No. please inquire). Ponceau S staining was used as a loading control. Western-Ready™ ECL Substrate Kit (Cat. No. please inquire) was used for developing.)
WB (Western Blot) (Western blot analysis of 15 ug cell lysates from DH5α cells. Samples were incubated at 37°C for 0, 5, 10, 15, and 40 minutes and then resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, probed with 2 ug/mL purified anti-Sigma 32 antibody, clone 3RH3, followed by incubation with horseradish peroxidase (HRP) labeled goat anti-mouse secondary antibody (Cat. No. please inquire). Ponceau S staining was used as a loading control. Western-Ready™ ECL Substrate Kit (Cat. No. please inquire) was used for developing.)
WB (Western Blot) (Figure-2: Western blot analysis of SPARCL1. Anti-SPARCL1 antibody (Clone: ABM5C3.2G1) was used at 1 ug/ml on (1) h Lung, (2) h Intestine, (3) PANC-28 and (4) U87 lysates.)
WB (Western Blot) (Figure-1: Western blot analysis of SPARCL1. Anti-SPARCL1 antibody (Clone: ABM5C3.2G1) was used at 1 ug/ml on (1) MCF-7, (2) A549, (3) HT-29, (4) HCT-116 and (5) PANC-1 lysates.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of RAB17 protein in paraffin embedded Human prostate tissue using RAB17 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant RAB17 protein (Right) or empty vector (Left) detected with RAB17 antibody)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant RAB17 protein using RAB17 antibody)
WB (Western Blot) (Figure 2. Western blot analysis of CD20/MS4A1 using anti-CD20/MS4A1 antibody (AAA126930).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Raji whole cell lysates,Lane 2: human Jurkat whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CD20/MS4A1 antigen affinity purified monoclonal antibody (#AAA126930) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD20/MS4A1 at approximately 37 kDa. The expected band size for CD20/MS4A1 is at 33 kDa.)
IF (Immunofluorescence) (Figure 1. IF analysis of CD20/MS4A1 using anti-CD20/MS4A1 antibody (AAA126930).CD20/MS4A1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 ug/mL rabbit anti-CD20/MS4A1 Antibody (AAA126930) overnight at 4 degree C. Biotin conjugated goat anti-mouse IgG (BA1001) was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using DyLight550 Conjugated Avidin (BA1134). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
FCM/FACS (Flow Cytometry) (Flow-cytometry using anti-human UPAR antibody. HUCCT cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human UPAR antibody monoclonal antibody (#AAA120763, Green Histogram) at a concentration of 5ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.)
WB (Western Blot) (Various lysates were subjected to SDS PAGE followed by western blot with CD87 antibody (AAA120763) at 1ug/ml.Lane 1: HUCCT cell lysateLane 2: U937 cell lysateLane 3: U251 cell lysateLane 4: A549 cell lysateLane 5: Hela cell lysateLane 6: HUVEC cell lysate Lane 7: Mouse brain lysateLane 8: Mouse kidney lysateLane 9: Mouse lung lysateLane 10: Rat brain lysateLane 11: Rat kidney lysateLane 12: Rat lung lysateSecond Ab: Goat Anti-Mouse IgG H&L Polyclonal antibody, HRP (PMB96431) at 0.1 ug/mL.Predict MW: 37 kDa)
FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of U87 cells using anti-UBE3A antibody (AAA125905).Overlay histogram showing U87 cells stained with AAA125905 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- UBE3A Antibody (AAA125905, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
WB (Western Blot) (Figure 1. Western blot analysis of UBE3A using anti-UBE3A antibody (AAA125905).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Jurkat whole cell lysatesLane 2: human HEK293 whole cell lysatesLane 3: human Raji whole cell lysatesLane 4: monkey COS-7 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- UBE3A antigen affinity purified monoclonal antibody (Catalog # AAA125905) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for UBE3A at approximately 100KD. The expected band size for UBE3A is at 100KD.)
WB (Western Blot) (Western blot analysis of PTH1R using anti-PTH1R antibody (AAA125141).Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human SH-SY5Y whole cell lysates,Lane 2: human A549 whole cell lysates,Lane 3: human SIHA whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTH1R antigen affinity purified monoclonal antibody (AAA125141) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate with Tanon 5200 system. A specific band was detected for PTH1R at approximately 66 kDa. The expected band size for PTH1R is at 66 kDa.)
WB (Western Blot) (Detection of human CK7 by western blot. Samples: Whole cell lysate (10 ug) from HEK293T, HCT 116, OVCAR-3, OVCAR-4, and A-549 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CK7 recombinant monoclonal antibody [BC1] (AAA213632 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
IP (Immunoprecipitation) (Detection of human CK7 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from A-549 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CK7 recombinant monoclonal antibody [BC1] (AAA213632 lot 1) used for IP at 20 ul/mg lysate. CK7 was also immunoprecipitated by another antibody against a different epitope of CK7. For blotting immunoprecipitated CK7, AAA213632 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 1 second.)
IHC (Immunohistochemisry) (Detection of human CK7 in breast carcinoma by IHC. Antibody: Rabbit anti-CK7 recombinant monoclonal antibody (AAA213632 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB)
ICC (Immunocytochemistry) (Detection of human CK7 in NCI-H1975 cells by ICC. Antibody: Rabbit anti-CK7 recombinant monoclonal antibody (AAA213632 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB)
FCM/FACS (Flow Cytometry) (Detection of human CK7 (shaded) in HeLa cells by flow cytometry. Antibody: Rabbit anti-CK7 recombinant monoclonal antibody [BC1] (AAA213632) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
Submit a Question
Please complete the form below and a representative will contact you as soon as possible.
Request more Information
Please complete the form below and a representative will contact you as soon as possible.
Request a Manual
Please complete the form below and a representative will contact you as soon as possible.
Request a Quote
Please complete the form below and a representative will contact you as soon as possible.