Secondary Antibodies

At AAA Biotech, we provide a vast collection of high-quality, purified, conjugated and unconjugated secondary antibodies to use in various applications, such as Western Blotting, Flow Cytometry, Cell Imaging, or CUT & Tag.

Our secondary antibodies are available with a wide range of labels—such as HRP, AP, FITC, and biotin—to match your detection system and enhance signal sensitivity. Each antibody is rigorously tested to ensure high specificity and minimal cross-reactivity, delivering reliable performance across multiple species and assay types.

Whether you're conducting qualitative imaging or quantitative analysis, AAA Biotech has the right secondary antibody to support your research with consistency and precision. Our entire catalog of secondary antibodies is available for browsing through our main website.

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IF (Immunofluorescence) (Detection of human p53 by immunofluorescence. Sample: FFPE section of human ovarian carcinoma. Primary Antibody: mouse anti-p53 (clone DO-1) used at a dilution of 1:100. Secondary Antibody: Green-fluorescent goat anti-mouse IgG-heavy and light chain Antibody DyLight 488 Conjugated (AAA213711 Lot 7) used at a dilution of 1:50 (10 ug/ml). Counterstain: DAPI (blue))

FCM/FACS (Flow Cytometry) (Detection of human CD20 (shaded) in Raji cells by flow cytometry. Antibody: Mouse anti-CD20 monoclonal antibody [L26] or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-mouse IgG (AAA213711).)

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IgG Heavy and Light Chain, Polyclonal Secondary Antibody (Cat# AAA213711)

Full Name

Goat anti-Mouse IgG Heavy and Light Chain Antibody DyLight 488 Conjugated

Reactivity

Mouse

Applications

Immunofluorescence, Flow Cytometry, Immunocytochemistry, Immunohistochemistry

Purity

Antigen Affinity Purified

Pricing

$280/0.5 mg $535/2x0.5 mg $780/3x0.5 mg $1,005/4x0.5 mg $1,015/5x0.5 mg

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IS (Immunostaining) (Immunostaining - C57BL/6J mouse retina labeled with: Green - 1st Ab Cone Arrestin (1/1,000); 2nd Ab goatAnti-rat (AB28488, 1/1,000), Red - 1st Ab Rhodopsin (1/1,000); 2nd Ab goatAnti-mouse (AB27550, 1/1,000), Blue - Nuclear staining (DAPI), Scale bar = 40 um;)

Reactivity Data

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IgG, Polyclonal Secondary Antibody (Cat# AAA63221)

Full Name

Anti-Rat IgG, DyLight488

Reactivity

Rat

Applications

Immunofluorescence, Western Blot

Purity

Epitope affinity purified

Pricing

$275/0.5 mg $525/2x0.5 mg $760/3x0.5 mg $985/4x0.5 mg $1,045/5x0.5 mg

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IF (Immunofluorescence) (Immunofluorescence - anti-mCherry Ab (AB9770) using hCEC cells transduced with mCherry-Rab5a; cells were fixed with methanol and mCherry Ab at 1/250; 2nd Ab goat anti-IgY (AB307633) at 1:1,000;)

IF (Immunofluorescence) (Immunofluorescence -Anti-mCherry Ab (AB9770) using hCEC cells transduced with mCherry-Rab5a; cells were fixed with methanol and mCherry Ab at 1/250; 2nd Ab goatAnti-IgY (AB307633) at 1:1,000;)

Reactivity Data

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IgY, Polyclonal Secondary Antibody (Cat# AAA63226)

Full Name

Anti-Chicken IgY, DyLight633

Reactivity

Chicken

Applications

Immunofluorescence, Western Blot

Purity

Epitope affinity purified

Pricing

$275/0.5 mg $525/2x0.5 mg $760/3x0.5 mg $985/4x0.5 mg $1,045/5x0.5 mg

ICC (Immunocytochemistry) (Immunocytochemical labeling of Fascin phosphorylation in aldehyde fixed rabbit fibroblast. The cells were labeled with rabbit polyclonal anti-Fascin (Ser-39) phosphospecific (FP2661). The antibody was detected using goat anti-rabbit DyLight 650.)

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Ig, Polyclonal Secondary Antibody (Cat# AAA71701)

Full Name

Anti-Rabbit Ig:DyLight 650 Secondary Antibody

Reactivity

Rabbit

Applications

Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Western Blot

Pricing

$300/0.5 mL $575/2x0.5 mL $835/3x0.5 mL $1,085/4x0.5 mL $1,125/5x0.5 mL

WB (Western Blot) (Western Blot of Goat IgG (H&L) antibody Peroxidase Conjugated Lane 1: Goat IgG Load: 100 ng per lane Goat IgG (H&L) antibody Peroxidase Conjugated at 1:1,000 for 60 min at RT Block: Blocking buffer for 30 min at RT)

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Rabbit anti Goat IgG (H + L) (HRP), Secondary Antibody (Cat# AAA75534)

Full Name

Rabbit anti Goat IgG (H + L) (HRP)

Reactivity

Goat IgG

Applications

Western Blot, Immunohistochemistry

Purity

Rabbit anti Goat IgG (H + L) (HRP) was purified by affinity chromatography.

Pricing

$685/2 mg $1,335/2x2 mg $1,945/3x2 mg $2,525/4x2 mg $2,855/5x2 mg

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IEP (Immunoelectrophoresis)

SDS-PAGE (1)Lot#27-52-0731122)Mol Weight Standard.)

Application Data (Goat anti-Llama IgG (H&L), Biotin Conjugate Test Data)

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IgG (H&L), Secondary Antibody (Cat# AAA79055)

Full Name

Goat anti-Llama IgG (H&L) - Affinity Pure, Biotin Conjugate

Reactivity

Llama.
Cross Reactivity: Based on IEP, no reactivity is observed to non-immunoglobulin llama serum immunoglobulins

Applications

Immunohistochemistry

Purity

Affinity purified antibody is > 95% based on SDS-PAGE
Affinity purified using solid phase Llama IgG.

Pricing

$410/1 mg $800/2x1 mg $1,160/3x1 mg $1,505/4x1 mg $1,655/5x1 mg

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IEP (Immunoelectrophoresis)

SDS-PAGE

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IgG (H&L), Secondary Antibody (Cat# AAA79056)

Full Name

Goat anti-Llama IgG (H&L) - Affinity Pure, DyLight488 Conjugate

Reactivity

Llama.
Cross Reactivity: Based on IEP, no reactivity is observed to non-immunoglobulin llama serum immunoglobulins

Applications

Immunoelectron Microscopy, Immunofluorescence, Flow Cytometry

Purity

Affinity purified antibody is > 95% based on SDS-PAGE
Affinity purified using solid phase Llama IgG.

Pricing

$385/1 mg $745/2x1 mg $1,090/3x1 mg $1,410/4x1 mg $1,525/5x1 mg

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Application Data (1) Lot# 16-188-0913112) Mol Weight Standard)

Application Data

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IgG (H&L), Secondary Antibody (Cat# AAA79058)

Full Name

Rabbit anti-Llama IgG (H&L) - Affinity Pure, ALP Conjugate

Reactivity

Llama.
Cross Reactivity: Based on IEP, no reactivity is observed to non-immunoglobulin llama serum immunoglobulins

Applications

Cleased Lysate, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Affinity purified antibody is > 95% based on SDS-PAGE
Affinity purified using solid phase Llama IgG.

Pricing

$410/0.5 mg $800/2x0.5 mg $1,160/3x0.5 mg $1,505/4x0.5 mg $1,655/5x0.5 mg

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IEP (Immunoelectrophoresis)

SDS-PAGE

Application Data

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IgG (H&L), Secondary Antibody (Cat# AAA79072)

Full Name

Donkey anti-Rabbit IgG (H&L) - Affinity Pure, DyLight350 Conjugate, min x w/bovine, chicken, goat, guinea pig, hamster, horse, human,mouse, rat or sheep IgG

Reactivity

Rabbit.
Cross Reactivity: Based on IEP, no reactivity is observed to:
non-immunoglobulin rabbit serum proteins
IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat or sheep

Applications

Immunoelectron Microscopy, Immunofluorescence, Flow Cytometry

Purity

Affinity purified antibody is > 95% based on SDS-PAGE
Affinity purified using solid phase Rabbit IgG.

Pricing

$355/1 mg $685/2x1 mg $995/3x1 mg $1,295/4x1 mg $1,380/5x1 mg

FCM/FACS (Flow Cytometry) (Human peripheral blood lymphocytes were stained with Goat Anti-Human IgD-PE and Mouse Anti -Human CD19-FITC)

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Goat Anti-Human IgD (delta chain specific), Polyclonal Secondary Antibody (Cat# AAA78793)

Full Name

Goat Anti-Human IgD-HRP

Reactivity

Human

Applications

Flow Cytometry

Purity

Affinity chromatography on human IgD covalently linked to agarose.

Pricing

$275/1 mL $525/2x1 mL $760/3x1 mL $985/4x1 mL $1,015/5x1 mL

What Are Secondary Antibodies?

Secondary antibodies are immunoglobulins that bind to primary antibodies, which are directly bound to the target antigen. They are conjugated with either an enzyme, fluorophore, or biotin, and they are almost always only targeting the constant region (Fc) of the primary antibody. As a result, this amplifies the signal in various assays and helps researchers detect, quantify, and visualize specific antigens in a complex biological sample.

Key Applications of Secondary Antibodies

• Western blotting for protein detection and quantification.


• ELISA (Enzyme-Linked Immunosorbent Assay) for antigen-antibody interaction studies and quantification.


• Immunohistochemistry (IHC) and immunofluorescence imaging for tissue and cell analysis.


• Flow cytometry for cell surface and intracellular marker detection.


• Immunoprecipitation (IP) and chromatin immunoprecipitation (ChIP) assays.


• Signal amplification in various immunoassays.


• Multiplex assays using fluorophore-conjugated secondary antibodies.

Advantages of Using Secondary Antibodies

1. High sensitivity

   
   

   Multiple secondary antibodies can bind to a single primary antibody and amplify the signal. This is especially useful for detecting antigens even if they are in low concentrations.

   
   



2. Increased flexibility in assay design

   
   

   Secondary antibodies come pre-conjugated with labels, such as enzymes, fluorophores, biotin, and more. This provides researchers with flexibility to use in multiple applications. They also support multiplexing for the simultaneous detection of multiple targets.

   
   



3. Cost-effective

   
   

   Since the Fc domain remains constant within the same animal class, only one type of secondary antibody is needed to bind many types of primary antibodies. This reduces the cost of labeling multiple primary antibodies.

   
   



4. Better signal-to-noise ratio

   
   

   Secondary antibodies minimize the background noise. As a result, they provide clearer and more accurate results.

   
   



5. Versatility

   
   

   A single species- and isotype-specific secondary antibody can be used with any compatible primary antibody. This can help streamline reagent inventory and reduce cost and preparation time.

   
   



6. Easier detection

   
   

   Unlike primary antibodies, secondary antibodies’ main characteristic is that they will almost always be partnered with a component that can be used for detection. This can make detection and visualization easy.

   
   

Why Buy Secondary Antibodies from AAA Biotech?

• Highly Validated: Most of our secondary antibodies are thoroughly tested to ensure they work reliably across different experiments.


• Versatile Applications: Our secondary antibodies can be used in a wide range of research techniques, including (but not limited to) immunocytochemistry (ICC), ELISA, immunofluorescence (IF), immunohistochemistry (IHC), flow cytometry (FC), immunoprecipitation (IP), and Western blotting (WB).


• Support for Rare Species: We offer secondary antibodies specifically designed for rare or less common species—something many other suppliers don’t provide.


• Affordable Prices: Our secondary antibodies are available at competitive prices to support all research budgets.


• Quick and Convenient Ordering: Simple online ordering process with responsive customer support.


• Privacy: We respect your privacy and ensure your information is protected at every step of the ordering process, and in all other communication with our team members.

FAQ

1. Can I reuse a secondary antibody?

Not recommended for critical experiments, as reusing may compromise sensitivity and specificity.

2. What is the difference between primary and secondary antibodies?

Primary antibodies bind directly to the antigen. Secondary antibodies bind to the primary and are usually labeled for detection.

3. How long do secondary antibodies last?

When stored properly (usually at –20°C or 4°C, depending on formulation), they can last for years. Always refer to the product datasheet.

4. What happens if you use too much secondary antibody?

Excess can increase background noise, leading to a poor signal-to-noise ratio. Titrate to find the optimal concentration.

5. Can secondary antibodies be monoclonal?

Yes. While many are polyclonal, monoclonal secondary antibodies are available for consistent batch-to-batch performance.

6. How to find secondary antibodies?

Use our catalog filters to search by host species, conjugate type, target species, and application.

7. How to store secondary antibodies?

Store at recommended temperatures away from light. Avoid repeated freeze-thaw cycles.