Secondary Antibodies

At AAA Biotech, we provide a vast collection of high-quality, purified, conjugated and unconjugated secondary antibodies to use in various applications, such as Western Blotting, Flow Cytometry, Cell Imaging, or CUT & Tag.

Our secondary antibodies are available with a wide range of labels—such as HRP, AP, FITC, and biotin—to match your detection system and enhance signal sensitivity. Each antibody is rigorously tested to ensure high specificity and minimal cross-reactivity, delivering reliable performance across multiple species and assay types.

Whether you're conducting qualitative imaging or quantitative analysis, AAA Biotech has the right secondary antibody to support your research with consistency and precision. Our entire catalog of secondary antibodies is available for browsing through our main website.

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Application Data (BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD45-UNLB followed by Goat Fab Anti-Rat Ig, Mouse ads-FITC)

FLISA (FLISA plate was coated with purified rat IgG and IgM. Immunoglobulins were detected with serially diluted Goat Fab Anti-Rat Ig, Mouse ads-FITC .)

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Fab Ig, Polyclonal Secondary Antibody (Cat# AAA78929)

Full Name

Goat Fab Anti-Rat Ig, Mouse ads-FITC

Reactivity

Rat

Applications

Flow Cytometry

Pricing

$315/0.5 mg $605/2x0.5 mg $880/3x0.5 mg $1,140/4x0.5 mg $1,200/5x0.5 mg

Application Data

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Goat Anti-Human kappa (kappa chain specific), Polyclonal Secondary Antibody (Cat# AAA78799)

Full Name

Goat Anti-Human Kappa-BIOT

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunoblot

Purity

Affinity chromatography on pooled human Igs with kappa light chains covalently linked to agarose.

Pricing

$285/1 mg $545/2x1 mg $795/3x1 mg $1,030/4x1 mg $1,075/5x1 mg

WB (Western Blot) (Total cell Iysates from Jurkat cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Rabbit Anti-Human DR5-UNlB . Proteins were visualized using Goat Anti-Rabbit IgG(H+L). Mouse/Human ads-HRP (AAA78823) secondary antibody and chemfluminescent detection.)

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Goat Anti-Rabbit IgG (H+L chain specific), Polyclonal Secondary Antibody (Cat# AAA78823)

Full Name

Goat Anti-Rabbit IgG (H+L), Mouse/Human ads-HRP

Reactivity

Rabbit

Applications

ELISA

Purity

Affinity chromatography on pooled IgG covalently linked to agarose.

Pricing

$225/1 mL $440/2x1 mL $645/3x1 mL $825/5x1 mL $835/4x1 mL

Application Data (ELISA plate was coated with purified chicken IgY and IgM. Immunoglobulins were detected with Goat Anti-Chicken IgY(H+L)-BIOT)

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Goat Anti-Chicken IgY (H+L), Polyclonal Secondary Antibody (Cat# AAA78838)

Full Name

Goat Anti-Chicken IgY (H+L)-HRP

Reactivity

Chicken

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunoblot

Purity

Affinity chromatography on chicken IgY covalently linked to agarose.

Pricing

$205/1 mL $400/2x1 mL $585/3x1 mL $730/5x1 mL $755/4x1 mL

ELISA (ELISA plate was coated with purified turkey IgY. Immunoglobulin was detected with Goat Anti-Turkey IgY (H+L)-HRP (AAA78839).)

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Goat Anti-Turkey IgY (H+L), Polyclonal Secondary Antibody (Cat# AAA78839)

Full Name

Goat Anti-Turkey IgY (H+L)-HRP

Reactivity

Turkey

Purity

Affinity chromatography on turkey IgY covalently linked to agarose.

Pricing

$205/1 mL $400/2x1 mL $585/3x1 mL $730/5x1 mL $755/4x1 mL

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ELISA (ELISA plate was coated with purified human IgM,IgG, and IgA. Immunoglobulins were detected with serially diluted Mouse F(ab')2 Anti-Human IgM-UNLB followed by Goat Anti-Mouse IgG(H+L), Human ads-HRP (AAA78714))

Application Data

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Mouse F(ab')2 Anti-Human IgM (u chain specific), Monoclonal Secondary Antibody (Cat# AAA78714)

Full Name

Mouse F(ab')2 Anti-Human IgM-UNLB

Reactivity

Human

Applications

Flow Cytometry

Pricing

$320/0.25 mg $615/2x0.25 mg $895/3x0.25 mg $1,160/4x0.25 mg $1,225/5x0.25 mg

Application Data (FLISA plate was coated with purified human IgG1, IgG2, IgG3, and IgG4. Immunoglobulins were detected with serially diluted Mouse Anti-Human IgG2 Fc-PE.)

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Mouse Anti-Human IgG2 (gamma 2 chain specific), Monoclonal Secondary Antibody (Cat# AAA78728)

Full Name

Mouse Anti-Human IgG2 (Fc)-PE

Reactivity

Human

Pricing

$375/0.1 mg $725/2x0.1 mg $1,060/3x0.1 mg $1,370/4x0.1 mg $1,485/5x0.1 mg

Application Data (FLISA plate was coated with purified human IgG1, IgG2, IgG3, and IgG4. Immunoglobulins were detected with serially diluted Mouse Anti-Human IgG3 Hinge-PE)

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Mouse Anti-Human IgG3 (gamma 3 chain specific), Monoclonal Secondary Antibody (Cat# AAA78743)

Full Name

Mouse Anti-Human IgG3 (hinge)-PE

Reactivity

Human

Pricing

$375/0.1 mg $725/2x0.1 mg $1,060/3x0.1 mg $1,370/4x0.1 mg $1,485/5x0.1 mg

ELISA (ELISA plate was coated with purified mouse IgA , IgG , and IgM. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgA-PE)

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Goat Anti-Mouse IgA (alpha chain specific), Polyclonal Secondary Antibody (Cat# AAA78772)

Full Name

Goat Anti-Mouse IgA-TRITC

Reactivity

Mouse

Applications

ELISA

Purity

Affinity chromatography on mouse IgA covalently linked to agarose.

Pricing

$335/1 mg $645/2x1 mg $940/3x1 mg $1,215/4x1 mg $1,290/5x1 mg

IF (Immunofluorescence) (Product: Goat Anti-Mouse IgG2a-FITCAmount used: )

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Goat Anti-Mouse IgG2a (gamma 2a chain specific), Polyclonal Secondary Antibody (Cat# AAA78780)

Full Name

Goat Anti-Mouse IgG2a, Human ads-HRP

Reactivity

Mouse

Applications

Flow Cytometry

Purity

Affinity chromatography on mouse IgG2a covalently linked to agarose.

Pricing

$285/1 mL $545/2x1 mL $795/3x1 mL $1,030/4x1 mL $1,065/5x1 mL

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IEP (Immunoelectrophoresis)

SDS-PAGE (1) Lot # 27-149-101413 2) Mol Weight Standard)

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IgG (H&L), Secondary Antibody (Cat# AAA79054)

Full Name

Rabbit anti-Human IgG (H&L) - Affinity Pure, ALP Conjugate

Reactivity

Human.
Cross Reactivity: Based on IEP, no reactivity is observed to non-immunoglobulin human serum immunoglobulins

Applications

Immunohistochemistry, Western Blot

Purity

Affinity purified antibody is > 95% based on SDS-PAGE
Affinity purified using solid phase Human IgG.

Pricing

$310/1 mg $595/2x1 mg $865/3x1 mg $1,120/4x1 mg $1,175/5x1 mg

WB (Western Blot) (Western blot All lanes: Mouse IgG Fab fragment antibody at 2 /ml +mouse serum Lane 1: mouse serum at 1: 100 Lane 2: mouse serum at 1: 1000 SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution)

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IgG Fab fragment, Secondary Antibody (Cat# AAA81481)

Full Name

Rabbit anti-Mouse IgG Fab fragment polyclonal Antibody

Reactivity

Mouse

Applications

ELISA

Purity

Caprylic Acid Ammonium Sulfate Precipitation Purified

Pricing

$235/0.5 mg $460/2x0.5 mg $670/3x0.5 mg $870/4x0.5 mg $1,055/5x0.5 mg

WB (Western Blot) ((Figure: Used in Western Blot; Sample: Mouse Serum.))

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IgG, Polyclonal Secondary Antibody (Cat# AAA146594)

Full Name

HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody

Reactivity

Mouse

Applications

Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Protein A + Protein G affinity chromatography

Pricing

$210/1 mL $415/5 mL $810/2x5 mL $1,175/3x5 mL $1,525/4x5 mL $1,840/5x5 mL

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Application Data (FLISA plate was coated with purified mouse IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted Rat Anti-Mouse IgM-PE)

Application Data (BALB/c mouse splenocytes were stained with Rat Anti-Mouse IgM-APC (AAA78648) and Rat Anti-Mouse CD19-FITC .)

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Rat Anti-Mouse IgM (u chain specific), Monoclonal Secondary Antibody (Cat# AAA78648)

Full Name

Rat Anti-Mouse IgM-APC

Reactivity

Mouse

Applications

Flow Cytometry

Pricing

$470/0.1 mg $920/2x0.1 mg $1,340/3x0.1 mg $1,735/4x0.1 mg $1,925/5x0.1 mg

ELISA

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Goat Anti-Mouse IgG (gamma chain specific), Polyclonal Secondary Antibody (Cat# AAA78766)

Full Name

Goat Anti-Mouse IgG, Human ads-UNLB

Reactivity

Mouse

Applications

ELISA

Purity

Affinity chromatography on pooled mouse IgG covalently linked to agarose.

Pricing

$210/1 mg $410/2x1 mg $600/3x1 mg $755/5x1 mg $775/4x1 mg

ELISA (ELISA plate was coated with purified human IgG1, IgG2, IgG3, and IgG4. Immunoglobulins were detected with serilly diluted Mouse Anti-Human IgG1 Fc-BIMA followed by Streptavidin-HRP)

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Rat Anti-Mouse IgG2b, Monoclonal Secondary Antibody (Cat# AAA78877)

Full Name

Rat Anti-Mouse IgG2b-PE

Reactivity

Human

Applications

ELISA

Pricing

$470/0.2 mg $920/2x0.2 mg $1,340/3x0.2 mg $1,735/4x0.2 mg $1,925/5x0.2 mg

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FCM/FACS (Flow Cytometry) (Human peripheral blood lymphocytes are stained with Anti-Human IgM Monoclonal Antibody(PE/Cyanine7 Conjugated)(filled gray histogram) or Mouse IgG1 Isotype Control PE/Cy7 (empty black histogram).)

Fluorophore (Conjugation: PE/Cyanime7)

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IgM, Monoclonal Secondary Antibody (Cat# AAA174757)

Full Name

Anti-Human IgM Monoclonal Antibody (PE/Cyanine7 Conjugated) [MHM-88]

Reactivity

Human

Applications

Flow Cytometry

Pricing

$195/20 Tests $355/100 Tests $490/2x100 Tests $2,165/10x100 Tests

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IS (Immunostaining) (Immunostaining - C57BL/6J mouse retina label with: Red - 1st Ab GRP78 BiP (1/250); 2nd Ab goat anti-rat (AB28550, 1/1000), Blue - Nuclear staining (DAPI), scale bar = 40 um;)

IS (Immunostaining) (Immunostaining - rd16 (retinal degeneration) mouse retina labeled with: Red - 1st Ab GFAP (1/500); 2nd Ab goatAnti-rat (AB28550, 1/1000), Blue - Nuclear staining (DAPI), scale bar = 40 um;)

Reactivity Data

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IgG, Polyclonal Secondary Antibody (Cat# AAA63222)

Full Name

Anti-Rat IgG, DyLight550

Reactivity

Rat

Applications

Immunofluorescence, Western Blot

Purity

Epitope affinity purified

Pricing

$275/0.5 mg $525/2x0.5 mg $760/3x0.5 mg $985/4x0.5 mg $1,045/5x0.5 mg

WB (Western Blot) ((Figure: Used in Western Blot; Sample: Gallus Serum.)Ab: 1:2000 Dilution of HRP-Linked Rabbit Anti-Chicken IgG AB (AAA146593))

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IgG, Secondary Antibody (Cat# AAA146593)

Full Name

HRP-Linked Rabbit Anti-Gallus IgG Polyclonal Antibody

Reactivity

Chicken

Applications

Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Antigen-specific affinity chromatography

Pricing

$210/1 mL $415/5 mL $810/2x5 mL $1,175/3x5 mL $1,525/4x5 mL $1,840/5x5 mL

What Are Secondary Antibodies?

Secondary antibodies are immunoglobulins that bind to primary antibodies, which are directly bound to the target antigen. They are conjugated with either an enzyme, fluorophore, or biotin, and they are almost always only targeting the constant region (Fc) of the primary antibody. As a result, this amplifies the signal in various assays and helps researchers detect, quantify, and visualize specific antigens in a complex biological sample.

Key Applications of Secondary Antibodies

• Western blotting for protein detection and quantification.


• ELISA (Enzyme-Linked Immunosorbent Assay) for antigen-antibody interaction studies and quantification.


• Immunohistochemistry (IHC) and immunofluorescence imaging for tissue and cell analysis.


• Flow cytometry for cell surface and intracellular marker detection.


• Immunoprecipitation (IP) and chromatin immunoprecipitation (ChIP) assays.


• Signal amplification in various immunoassays.


• Multiplex assays using fluorophore-conjugated secondary antibodies.

Advantages of Using Secondary Antibodies

1. High sensitivity

   
   

   Multiple secondary antibodies can bind to a single primary antibody and amplify the signal. This is especially useful for detecting antigens even if they are in low concentrations.

   
   



2. Increased flexibility in assay design

   
   

   Secondary antibodies come pre-conjugated with labels, such as enzymes, fluorophores, biotin, and more. This provides researchers with flexibility to use in multiple applications. They also support multiplexing for the simultaneous detection of multiple targets.

   
   



3. Cost-effective

   
   

   Since the Fc domain remains constant within the same animal class, only one type of secondary antibody is needed to bind many types of primary antibodies. This reduces the cost of labeling multiple primary antibodies.

   
   



4. Better signal-to-noise ratio

   
   

   Secondary antibodies minimize the background noise. As a result, they provide clearer and more accurate results.

   
   



5. Versatility

   
   

   A single species- and isotype-specific secondary antibody can be used with any compatible primary antibody. This can help streamline reagent inventory and reduce cost and preparation time.

   
   



6. Easier detection

   
   

   Unlike primary antibodies, secondary antibodies’ main characteristic is that they will almost always be partnered with a component that can be used for detection. This can make detection and visualization easy.

   
   

Why Buy Secondary Antibodies from AAA Biotech?

• Highly Validated: Most of our secondary antibodies are thoroughly tested to ensure they work reliably across different experiments.


• Versatile Applications: Our secondary antibodies can be used in a wide range of research techniques, including (but not limited to) immunocytochemistry (ICC), ELISA, immunofluorescence (IF), immunohistochemistry (IHC), flow cytometry (FC), immunoprecipitation (IP), and Western blotting (WB).


• Support for Rare Species: We offer secondary antibodies specifically designed for rare or less common species—something many other suppliers don’t provide.


• Affordable Prices: Our secondary antibodies are available at competitive prices to support all research budgets.


• Quick and Convenient Ordering: Simple online ordering process with responsive customer support.


• Privacy: We respect your privacy and ensure your information is protected at every step of the ordering process, and in all other communication with our team members.

FAQ

1. Can I reuse a secondary antibody?

Not recommended for critical experiments, as reusing may compromise sensitivity and specificity.

2. What is the difference between primary and secondary antibodies?

Primary antibodies bind directly to the antigen. Secondary antibodies bind to the primary and are usually labeled for detection.

3. How long do secondary antibodies last?

When stored properly (usually at –20°C or 4°C, depending on formulation), they can last for years. Always refer to the product datasheet.

4. What happens if you use too much secondary antibody?

Excess can increase background noise, leading to a poor signal-to-noise ratio. Titrate to find the optimal concentration.

5. Can secondary antibodies be monoclonal?

Yes. While many are polyclonal, monoclonal secondary antibodies are available for consistent batch-to-batch performance.

6. How to find secondary antibodies?

Use our catalog filters to search by host species, conjugate type, target species, and application.

7. How to store secondary antibodies?

Store at recommended temperatures away from light. Avoid repeated freeze-thaw cycles.