Secondary Antibodies

At AAA Biotech, we provide a vast collection of high-quality, purified, conjugated and unconjugated secondary antibodies to use in various applications, such as Western Blotting, Flow Cytometry, Cell Imaging, or CUT & Tag.

Our secondary antibodies are available with a wide range of labels—such as HRP, AP, FITC, and biotin—to match your detection system and enhance signal sensitivity. Each antibody is rigorously tested to ensure high specificity and minimal cross-reactivity, delivering reliable performance across multiple species and assay types.

Whether you're conducting qualitative imaging or quantitative analysis, AAA Biotech has the right secondary antibody to support your research with consistency and precision. Our entire catalog of secondary antibodies is available for browsing through our main website.

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Application Data (Rabbit peripheral blood lymphocytes were stained with Goat Anti-Rabbit IgG(H+L), Human ads-FITC)

FLISA (FLISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Goat Anti-Rabbit IgG(H+L), Human ads-FITC)

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IgG, Polyclonal Secondary Antibody (Cat# AAA78930)

Full Name

Goat Anti-Rabbit IgG(H+L), Human ads-FITC

Reactivity

Rabbit

Applications

FLISA, ELISA

Purity

Affinity chromatography on pooled rabbit IgG covalently linked to agarose

Pricing

$200/1 mg $390/2x1 mg $570/3x1 mg $715/5x1 mg $735/4x1 mg

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IF (Immunofluorescence) (Immunofluorescence - anti-mCherry Ab (AB9770) using hCEC cells transduced with mCherry-Rab5a; cells were fixed with methanol and mCherry Ab at 1/250; 2nd Ab goat anti-IgY (AB307405) at 1:1,000;)

IF (Immunofluorescence) (Immunofluorescence -Anti-mCherry Ab (AB9770) using hCEC cells transduced with mCherry-Rab5a; cells were fixed with methanol and mCherry Ab at 1/250; 2nd Ab goatAnti-IgY (AB307405) at 1:1,000;)

Reactivity Data

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IgY, Polyclonal Secondary Antibody (Cat# AAA63223)

Full Name

Anti-Chicken IgY, DyLight405

Reactivity

Chicken

Applications

Immunofluorescence, Western Blot

Purity

Epitope affinity purified

Pricing

$275/0.5 mg $525/2x0.5 mg $760/3x0.5 mg $985/4x0.5 mg $1,045/5x0.5 mg

WB (Western Blot) ((Figure: Used in Western Blot, Sample: Rabbit Serum.))

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IgG, Secondary Antibody (Cat# AAA146596)

Full Name

HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

Reactivity

Rabbit

Applications

Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Antigen-specific Affinity Chromatography.

Pricing

$210/1 mL $415/5 mL $810/2x5 mL $1,175/3x5 mL $1,525/4x5 mL $1,840/5x5 mL

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WB (Western Blot) (Detection of GAPDH and hnRNP in HeLa Whole Cell Lysate. Primary Antibodies: cocktail of goat anti-GAPDH and mouse anti-hnRNP at 1 ug/ml each. Secondary Antibodies: cocktail of Dylight 680-conjugated rabbit anti-goat (red) and Dylight 488-conjugated rabbit anti-mouse AAA213715 (AAA213715-3) (blue) at 0.5 ug/ml each. Acquisition: Syngene G:Box, 39 seconds (red) and 60 seconds (blue).)

IF (Immunofluorescence) (Detection of human p53 by immunofluorescence. Sample: FFPE section of human breast carcinoma. Primary Antibody: mouse anti-p53 (clone DO-1) used at a dilution of 1:100. Secondary Antibody: Green-fluorescent Rabbit anti-mouse IgG-heavy and light chain cross-adsorbed Antibody DyLight 488 Conjugated (AAA213715 Lot 6) used at a dilution of 1:100 (5ug/ml). Counterstain: DAPI (blue))

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IgG Heavy and Light Chain Cross-Adsorbed, Polyclonal Secondary Antibody (Cat# AAA213715)

Full Name

Rabbit anti-Mouse IgG Heavy and Light Chain Cross-Adsorbed Antibody DyLight 488 Conjugated

Reactivity

Mouse
Minimum Reactivity: Human, Rat

Applications

Immunofluorescence, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purified

Pricing

$290/0.5 mg $555/2x0.5 mg $805/3x0.5 mg $1,045/4x0.5 mg $1,060/5x0.5 mg

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IF (Immunofluorescence) (Immunofluorescence in Drosophila larvaenmJ muscle 6/7 expressing GluRIIA-GFP in neurons (GluRIIA is a post-synaptic protein) using 1st Ab anti-GFP at 1/1,000 and 2nd Ab anti-goat IgY conjugated to DyLight550 at 1/500;)

Application Data (Anti-GAPDH Ab at 1/2,500 dilution using HEK293 transfected cell lysates at 50 ug per lane; chicken polyclonal to goat IgG conjugated to DyLight 550 (AB200550) at 1/10,000 dilution;)

Reactivity Data

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IgG, Polyclonal Secondary Antibody (Cat# AAA63211)

Full Name

Anti-Goat IgG, DyLight550

Reactivity

Goat

Applications

Immunofluorescence, Western Blot

Purity

Epitope affinity purified

Pricing

$275/0.5 mg $525/2x0.5 mg $760/3x0.5 mg $985/4x0.5 mg $1,045/5x0.5 mg

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Application Data (This table displays additional reactivity among various species of serum and IgA (+) indicates antibody reactivity to the corresponding target.)

WB (Western Blot) (Western Blot of Peroxidase conjugated Monkey IgG gamma antibody. Lane 1: Monkey IgG. Lane 2: none. Load: 50 ng per lane. none. Peroxidase Goat secondary antibody at 1:1,000 for 30 min at RT. Block: Blocking buffer for 30 min at RT. Size: 55 kDa for Monkey IgG Gamma..)

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Goat anti Monkey IgG (HRP), Secondary Antibody (Cat# AAA75523)

Full Name

Goat anti Monkey IgG (HRP)

Applications

Immunohistochemistry, Western Blot

Purity

Goat anti Monkey IgG (HRP) was purified by delipidation, salt fractionation and ion exchange chromatography.

Pricing

$920/1 mg $1,795/2x1 mg $2,615/3x1 mg $3,395/4x1 mg $3,905/5x1 mg

ELISA (Lane 1: Rabbit IgGLane 2: Rabbit IgG Light ChainsLane 3: Rabbit IgG Heavy ChainsRabbit immunoglobulins above were resolved by electrophoresis under reducing conditions, transfered to PVDF membrane , and probed with Mouse Anti-Rabbit IgG HRP . Proteins were visulized using chemiluminescent detection.)

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Mouse Anti-Rabbit IgG, Monoclonal Secondary Antibody (Cat# AAA78700)

Full Name

Mouse Anti-Rabbit IgG-BIOT

Reactivity

Rabbit

Applications

Flow Cytometry

Pricing

$320/0.5 mg $615/2x0.5 mg $895/3x0.5 mg $1,160/4x0.5 mg $1,225/5x0.5 mg

IF (Immunofluorescence)

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Goat Anti-Mouse IgM+IgG+IgA(H+L), Polyclonal Secondary Antibody (Cat# AAA78762)

Full Name

Goat Anti-Mouse Ig, Human ads-BGAL

Reactivity

Mouse

Applications

Immunostaining, Immunoblot

Purity

Affinity chromatography on pooled mouse IgM+IgG+IgA covalently linked to agarose.

Pricing

$245/1 mL $480/2x1 mL $700/3x1 mL $910/4x1 mL $915/5x1 mL

Application Data (ELISA plate was coated with purified goat IgG Fc and IgG Fab. Immunoglobulins were detected with serially diluted Rabbit Anti-Goat IgG Fc-HRP (SB Cat. No.AAA78879).)

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Rabbit Anti-Goat IgG Fc, Polyclonal Secondary Antibody (Cat# AAA78879)

Full Name

Rabbit Anti-Goat IgG Fc-HRP

Applications

ELISA

Purity

Affinity chromatography on goat IgG covalently linked to agarose

Pricing

$210/1 mL $410/2x1 mL $600/3x1 mL $750/5x1 mL $775/4x1 mL

SDS-PAGE

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IgY (H&L), Secondary Antibody (Cat# AAA79073)

Full Name

Goat anti-Chicken IgY (H&L) - Affinity Pure, DyLight350 Conjugate

Reactivity

Chicken.
Cross Reactivity: Based on IEP, no reactivity is observed to non-immunoglobulin chicken serum immunoglobulins

Applications

Immunoelectron Microscopy, Immunofluorescence, Flow Cytometry

Purity

Affinity purified antibody is > 95% based on SDS-PAGE
Affinity purified using solid phase Chicken IgG/Y.

Pricing

$240/1 mg $470/2x1 mg $685/3x1 mg $890/4x1 mg $895/5x1 mg

Application Data

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Rat Anti-Mouse IgG2a (gamma 2a chain specific), Monoclonal Secondary Antibody (Cat# AAA78649)

Full Name

Rat Anti-Mouse IgG2a-FITC

Reactivity

Mouse

Applications

Flow Cytometry

Pricing

$345/0.5 mg $665/2x0.5 mg $965/3x0.5 mg $1,255/4x0.5 mg $1,340/5x0.5 mg

Application Data (Human peripheral blood lymphocytes were stained with Goat Anti-Human IgM-PE and Mouse Anti-human CD19-FITC)

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Goat Anti-Human IgM (u chain specific), Secondary Antibody (Cat# AAA78787)

Full Name

Goat Anti-Human IgM-AP

Reactivity

Human

Applications

ELISA

Purity

Affinity chromatography on human IgM covalently linked to agarose

Pricing

$275/1 mL $525/2x1 mL $760/3x1 mL $985/4x1 mL $1,020/5x1 mL

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IF (Immunofluorescence) (Immunofluorescence using hCEC cells, 1st Ab (anti-beta-Actin at 1/250) and 2nd Ab (anti-mouse AB27405 at 1/1,000); cells were fixed with methanol;)

Reactivity Data

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IgG, Polyclonal Secondary Antibody (Cat# AAA63217)

Full Name

Anti-Mouse IgG, DyLight405

Reactivity

Mouse

Applications

Immunofluorescence, Western Blot

Purity

Epitope affinity purified

Pricing

$275/0.5 mg $525/2x0.5 mg $760/3x0.5 mg $985/4x0.5 mg $1,045/5x0.5 mg

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IF (Immunofluorescence) (Immunofluorescence using hCEC cells, 1st Ab (anti-beta-Actin at 1/250) and 2nd Ab (anti-mouse AB27633 at 1/1,000); cells were fixed with methanol;)

Reactivity Data

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IgG, Polyclonal Secondary Antibody (Cat# AAA63220)

Full Name

Anti-Mouse IgG, DyLight633

Reactivity

Mouse

Applications

Immunofluorescence, Western Blot

Purity

Epitope affinity purified

Pricing

$275/0.5 mg $525/2x0.5 mg $760/3x0.5 mg $985/4x0.5 mg $1,045/5x0.5 mg

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IF (Immunofluorescence) (Immunofluorescence -Anti-mCherry Ab (AB9770) using hCEC cells transduced with mCherry-Rab5a; cells were fixed with methanol and mCherry Ab at 1/250; 2nd Ab goatAnti-IgY (AB307633) at 1:1,000;)

Reactivity Data

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IgY, Polyclonal Secondary Antibody (Cat# AAA63225)

Full Name

Anti-Chicken IgY, DyLight550

Reactivity

Chicken

Applications

Immunofluorescence, Western Blot

Purity

Epitope affinity purified

Pricing

$275/0.5 mg $525/2x0.5 mg $760/3x0.5 mg $985/4x0.5 mg $1,045/5x0.5 mg

DB (Dot Blot) (Dot Blot analysis using Goat anti Rabbit IgG FITC and HRP conj ugated secondary antibody used to detect nanogram - picogram levels of rabbit IgG by dot blot on nitrocellulose membrane. 4 ul each of serial 1 in 4 dilutions of rabbit IgG were dotted on nitrocellulose and allowed to dry. Membrane was blocked in 3% BSA for 10 minutes dried for later use and rewetted with blocking buffer.)

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Goat anti Rabbit IgG (H + L) (FITC), Secondary Antibody (Cat# AAA75483)

Full Name

Goat anti Rabbit IgG (H + L) (FITC)

Reactivity

Rabbit IgG (H + L)

Applications

Immunofluorescence, Flow Cytometry

Purity

Goat anti Rabbit IgG (H + L) (FITC) was purified by affinity chromatography.

Pricing

$525/2 mg $1,015/2x2 mg $1,485/3x2 mg $1,930/4x2 mg $2,130/5x2 mg

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DB (Dot Blot) (Dot Blot of Rhodamine Conjugated Goat-anti-Rabbit IgG. Antigen: Rabbit IgG. Load: Lane 1 - 50ng Lane 2 - 16.67ng Lane 3 - 5.56ng Lane 4 - 1.85ng Lane 5 - 0.62ng. Primary antibody: none. Secondary antibody: Rhodamine Conjugated Goat-a-Rabbit IgG secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 60 min at RT.)

WB (Western Blot) (Western Blot of Rabbit IgG Rhodamine Conjugated Secondary antibody. Lane 1: Rabbit IgG. Lane 2: None. Load: 50 ng per lane. None. Rhodamine Goat secondary antibody at 1:1,000 for 60 min at RT. Block: Blocking buffer for 30 min at RT. Size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG..)

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Goat anti Rabbit IgG (H + L) (rhodamine), Secondary Antibody (Cat# AAA75484)

Full Name

Goat anti Rabbit IgG (H + L) (rhodamine)

Reactivity

Rabbit IgG (H + L)

Applications

Immunofluorescence, Flow Cytometry

Purity

Goat anti Rabbit IgG (H + L) (Rhodamine) was purified by affinity chromatography.

Pricing

$520/2 mg $1,005/2x2 mg $1,470/3x2 mg $1,910/4x2 mg $2,120/5x2 mg

WB (Western Blot)

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Goat anti Human IgG (H + L) (FITC), Secondary Antibody (Cat# AAA75619)

Full Name

Goat anti Human IgG (H + L) (FITC)

Reactivity

Assay by immunoelcerophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-goat serum, Human serum.

Applications

Immunofluorescence, Flow Cytometry

Purity

Goat anti Human IgG (H + L) (FITC) was purified by affinity chromatography.

Pricing

$540/2 mg $1,040/2x2 mg $1,515/3x2 mg $1,965/4x2 mg $2,180/5x2 mg

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DB (Dot Blot) (Antigen: Rabbit IgG. Load: Lane 1 - 100 ng Lane 2 - 33.3 ng Lane 3 - 11.1 ng Lane 4 - 3.70 ng Lane 5 - 1.23 ng. Goat Fab'2 anti-Rabbit IgG Antibody (FITC) at 1:1,000 for 1 HR at RT. Block for 1 HR at RT.)

WB (Western Blot) (Western blot of FITC conjugated Goat F(ab')2 Anti-Rabbit IgG. Lane 1: Rabbit IgG. Lane 2: None. Load: 50 ng per lane. None. FITC Goat secondary antibody at 1:1,000 for 60 min at RT. Blocking: Blocking buffer for 30 min at RT. Size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG..)

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Goat anti Rabbit IgG (H + L) (Fab'2) (FITC), Secondary Antibody (Cat# AAA75502)

Full Name

Goat anti Rabbit IgG (H + L) (Fab'2) (FITC)

Reactivity

Rabbit IgG (H + L)

Applications

Immunofluorescence, Flow Cytometry

Purity

Goat anti Rabbit IgG (H + L) (Fab'2) (FITC) was purified by affinity chromatography.

Pricing

$700/0.5 mg $1,365/2x0.5 mg $1,990/3x0.5 mg $2,585/4x0.5 mg $2,930/5x0.5 mg

What Are Secondary Antibodies?

Secondary antibodies are immunoglobulins that bind to primary antibodies, which are directly bound to the target antigen. They are conjugated with either an enzyme, fluorophore, or biotin, and they are almost always only targeting the constant region (Fc) of the primary antibody. As a result, this amplifies the signal in various assays and helps researchers detect, quantify, and visualize specific antigens in a complex biological sample.

Key Applications of Secondary Antibodies

• Western blotting for protein detection and quantification.


• ELISA (Enzyme-Linked Immunosorbent Assay) for antigen-antibody interaction studies and quantification.


• Immunohistochemistry (IHC) and immunofluorescence imaging for tissue and cell analysis.


• Flow cytometry for cell surface and intracellular marker detection.


• Immunoprecipitation (IP) and chromatin immunoprecipitation (ChIP) assays.


• Signal amplification in various immunoassays.


• Multiplex assays using fluorophore-conjugated secondary antibodies.

Advantages of Using Secondary Antibodies

1. High sensitivity

   
   

   Multiple secondary antibodies can bind to a single primary antibody and amplify the signal. This is especially useful for detecting antigens even if they are in low concentrations.

   
   



2. Increased flexibility in assay design

   
   

   Secondary antibodies come pre-conjugated with labels, such as enzymes, fluorophores, biotin, and more. This provides researchers with flexibility to use in multiple applications. They also support multiplexing for the simultaneous detection of multiple targets.

   
   



3. Cost-effective

   
   

   Since the Fc domain remains constant within the same animal class, only one type of secondary antibody is needed to bind many types of primary antibodies. This reduces the cost of labeling multiple primary antibodies.

   
   



4. Better signal-to-noise ratio

   
   

   Secondary antibodies minimize the background noise. As a result, they provide clearer and more accurate results.

   
   



5. Versatility

   
   

   A single species- and isotype-specific secondary antibody can be used with any compatible primary antibody. This can help streamline reagent inventory and reduce cost and preparation time.

   
   



6. Easier detection

   
   

   Unlike primary antibodies, secondary antibodies’ main characteristic is that they will almost always be partnered with a component that can be used for detection. This can make detection and visualization easy.

   
   

Why Buy Secondary Antibodies from AAA Biotech?

• Highly Validated: Most of our secondary antibodies are thoroughly tested to ensure they work reliably across different experiments.


• Versatile Applications: Our secondary antibodies can be used in a wide range of research techniques, including (but not limited to) immunocytochemistry (ICC), ELISA, immunofluorescence (IF), immunohistochemistry (IHC), flow cytometry (FC), immunoprecipitation (IP), and Western blotting (WB).


• Support for Rare Species: We offer secondary antibodies specifically designed for rare or less common species—something many other suppliers don’t provide.


• Affordable Prices: Our secondary antibodies are available at competitive prices to support all research budgets.


• Quick and Convenient Ordering: Simple online ordering process with responsive customer support.


• Privacy: We respect your privacy and ensure your information is protected at every step of the ordering process, and in all other communication with our team members.

FAQ

1. Can I reuse a secondary antibody?

Not recommended for critical experiments, as reusing may compromise sensitivity and specificity.

2. What is the difference between primary and secondary antibodies?

Primary antibodies bind directly to the antigen. Secondary antibodies bind to the primary and are usually labeled for detection.

3. How long do secondary antibodies last?

When stored properly (usually at –20°C or 4°C, depending on formulation), they can last for years. Always refer to the product datasheet.

4. What happens if you use too much secondary antibody?

Excess can increase background noise, leading to a poor signal-to-noise ratio. Titrate to find the optimal concentration.

5. Can secondary antibodies be monoclonal?

Yes. While many are polyclonal, monoclonal secondary antibodies are available for consistent batch-to-batch performance.

6. How to find secondary antibodies?

Use our catalog filters to search by host species, conjugate type, target species, and application.

7. How to store secondary antibodies?

Store at recommended temperatures away from light. Avoid repeated freeze-thaw cycles.