Secondary Antibodies

At AAA Biotech, we provide a vast collection of high-quality, purified, conjugated and unconjugated secondary antibodies to use in various applications, such as Western Blotting, Flow Cytometry, Cell Imaging, or CUT & Tag.

Our secondary antibodies are available with a wide range of labels—such as HRP, AP, FITC, and biotin—to match your detection system and enhance signal sensitivity. Each antibody is rigorously tested to ensure high specificity and minimal cross-reactivity, delivering reliable performance across multiple species and assay types.

Whether you're conducting qualitative imaging or quantitative analysis, AAA Biotech has the right secondary antibody to support your research with consistency and precision. Our entire catalog of secondary antibodies is available for browsing through our main website.

FLISA

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Mouse Anti-Human IgG3 (gamma 3 chain specific), Secondary Antibody (Cat# AAA78739)

Full Name

Mouse Anti-Human IgG3 (hinge)-FITC

Reactivity

Human

Applications

FLISA

Pricing

$360/0.5 mg $695/2x0.5 mg $1,010/3x0.5 mg $1,315/4x0.5 mg $1,425/5x0.5 mg

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IEP (Immunoelectrophoresis)

SDS-PAGE (1)Lot#27-52-0731122)Mol Weight Standard.)

Application Data (Goat anti-Llama IgG (H&L), Biotin Conjugate Test Data)

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IgG (H&L), Secondary Antibody (Cat# AAA79055)

Full Name

Goat anti-Llama IgG (H&L) - Affinity Pure, Biotin Conjugate

Reactivity

Llama.
Cross Reactivity: Based on IEP, no reactivity is observed to non-immunoglobulin llama serum immunoglobulins

Applications

Immunohistochemistry

Purity

Affinity purified antibody is > 95% based on SDS-PAGE
Affinity purified using solid phase Llama IgG.

Pricing

$410/1 mg $800/2x1 mg $1,160/3x1 mg $1,505/4x1 mg $1,655/5x1 mg

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IEP (Immunoelectrophoresis)

SDS-PAGE

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IgG (H&L), Secondary Antibody (Cat# AAA79056)

Full Name

Goat anti-Llama IgG (H&L) - Affinity Pure, DyLight488 Conjugate

Reactivity

Llama.
Cross Reactivity: Based on IEP, no reactivity is observed to non-immunoglobulin llama serum immunoglobulins

Applications

Immunoelectron Microscopy, Immunofluorescence, Flow Cytometry

Purity

Affinity purified antibody is > 95% based on SDS-PAGE
Affinity purified using solid phase Llama IgG.

Pricing

$385/1 mg $745/2x1 mg $1,090/3x1 mg $1,410/4x1 mg $1,525/5x1 mg

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Application Data (1) Lot# 16-188-0913112) Mol Weight Standard)

Application Data

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IgG (H&L), Secondary Antibody (Cat# AAA79058)

Full Name

Rabbit anti-Llama IgG (H&L) - Affinity Pure, ALP Conjugate

Reactivity

Llama.
Cross Reactivity: Based on IEP, no reactivity is observed to non-immunoglobulin llama serum immunoglobulins

Applications

Cleased Lysate, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Affinity purified antibody is > 95% based on SDS-PAGE
Affinity purified using solid phase Llama IgG.

Pricing

$410/0.5 mg $800/2x0.5 mg $1,160/3x0.5 mg $1,505/4x0.5 mg $1,655/5x0.5 mg

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IEP (Immunoelectrophoresis)

SDS-PAGE

Application Data

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IgG (H&L), Secondary Antibody (Cat# AAA79072)

Full Name

Donkey anti-Rabbit IgG (H&L) - Affinity Pure, DyLight350 Conjugate, min x w/bovine, chicken, goat, guinea pig, hamster, horse, human,mouse, rat or sheep IgG

Reactivity

Rabbit.
Cross Reactivity: Based on IEP, no reactivity is observed to:
non-immunoglobulin rabbit serum proteins
IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat or sheep

Applications

Immunoelectron Microscopy, Immunofluorescence, Flow Cytometry

Purity

Affinity purified antibody is > 95% based on SDS-PAGE
Affinity purified using solid phase Rabbit IgG.

Pricing

$355/1 mg $685/2x1 mg $995/3x1 mg $1,295/4x1 mg $1,380/5x1 mg

ELISA (ELISA plate was coated with purified human IgA, IgG, and IgM. Immunoglobulins were detected with serially diluted Goat Anti-Human IgA-HRP (AAA78796).)

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Goat Anti-Human IgA (alpha chain specific), Polyclonal Secondary Antibody (Cat# AAA78796)

Full Name

Goat Anti-Human IgA-HRP

Reactivity

Human IgG and IgM; may react with IgA from other species.

Applications

Flow Cytometry

Purity

Affinity chromatography on human IgA covalently linked to agarose

Pricing

$225/1 mL $440/2x1 mL $645/3x1 mL $825/5x1 mL $835/4x1 mL

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Application Data (FLISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat F(ab’)2 Anti-Human Kappa-FITC)

Application Data

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Goat F(ab')2 Anti-Human kappa (kappa chain specific), Polyclonal Secondary Antibody (Cat# AAA78802)

Full Name

Goat F(ab')2 Anti-Human Kappa-UNLB

Reactivity

Human

Applications

Western Blot, Flow Cytometry

Purity

Affinity chromatography on pooled human Igs with kappa light chains covalently linked to agarose.

Pricing

$265/0.5 mg $510/2x0.5 mg $745/3x0.5 mg $965/4x0.5 mg $1,000/5x0.5 mg

Application Data (FliSA plate was coated with purified rat IgM and IgG. Immunoglobulins were detected with serially diluted Goat Anti-Rat IgM-FITC)

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Goat Anti-Rat IgM (u chain specific), Polyclonal Secondary Antibody (Cat# AAA78810)

Full Name

Goat Anti-Rat IgM-HRP

Reactivity

Rat

Applications

ELISA

Purity

Affinity chromatography on pooled rat IgM covalently linked to agarose

Pricing

$240/1 mL $470/2x1 mL $685/3x1 mL $890/5x1 mL $890/4x1 mL

Application Data

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Goat Anti-Rat IgM (u chain specific), Polyclonal Secondary Antibody (Cat# AAA78811)

Full Name

Goat Anti-Rat IgM-BIOT

Reactivity

Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunoblot

Purity

Affinity chromatography on rat IgM covalently linked to agarose

Pricing

$250/1 mg $490/2x1 mg $715/3x1 mg $930/4x1 mg $935/5x1 mg

Application Data (Purified rabbit IgG was resolved by electrophoresis, transferred to PDVF membrane, and visualized using Goat Anti-Rabbit IgG-HRP secondary antibody and chemiluminescent detection.)

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Goat anti Rabbit IgG, Polyclonal Secondary Antibody (Cat# AAA78820)

Full Name

Goat Anti-Rabbit IgG-AP

Reactivity

Rabbit

Applications

Immunohistochemistry, Immunofluorescence, Immunoblot

Purity

Affinity chromatography on rabbit IgG covalently linked to agarose

Pricing

$265/1 mL $510/2x1 mL $745/3x1 mL $965/4x1 mL $1,000/5x1 mL

ELISA

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Rabbit Anti-Mouse IgG (H+L), Polyclonal Secondary Antibody (Cat# AAA78844)

Full Name

Rabbit Anti-Mouse IgG (H+L)-FITC

Reactivity

Mouse

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunoblot

Purity

Affinity chromatography on pooled mouse IgG covalently linked to agarose.

Pricing

$195/1 mg $380/2x1 mg $555/3x1 mg $690/5x1 mg $715/4x1 mg

ELISA (ELISA plate was coated with purified rat IgG and IgM, Immunoglobulins were detected with Rabbit Anti-Rat IgG (H+L) BIOT followed by Streptavidin- HRP)

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Rabbit Anti-Rat IgG (H+L), Polyclonal Secondary Antibody (Cat# AAA78848)

Full Name

Rabbit Anti-Rat IgG (H+L)-HRP

Reactivity

Rat

Purity

Affinity chromatography on pooled rat IgG covalently linked to agarose.

Pricing

$215/1 mL $420/2x1 mL $615/3x1 mL $775/5x1 mL $795/4x1 mL

IHC (Immunohistochemistry) (Paraffin embedded human gastric cancer tissue was stained with Goat 19G-UNlB isotype control and Goat Anti -Type III Coliagen·UNLB followed by Swine Anti·Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP , DAB, and hematoxylin.)

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Swine Anti-Goat IgG (H+L), Polyclonal Secondary Antibody (Cat# AAA78852)

Full Name

Swine Anti-Goat IgG (H+L), Human/Rat/Mouse ads-FITC

Reactivity

Goat

Applications

FLISA

Purity

Affinity chromatography on goat IgG covalently linked to agarose.

Pricing

$235/1 mg $460/2x1 mg $670/3x1 mg $870/4x1 mg $875/5x1 mg

Application Data

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IgG (H&L), Secondary Antibody (Cat# AAA79069)

Full Name

Goat anti-Donkey IgG (H&L) - Affinity Pure, DyLight633 Conjugate

Reactivity

Donkey.
Cross Reactivity: Based on IEP, no reactivity is observed to non-immunoglobulin donkey serum immunoglobulins

Applications

Immunoelectron Microscopy, Immunofluorescence, Flow Cytometry

Purity

Affinity purified antibody is > 95% based on SDS-PAGE
Affinity purified using solid phase Donkey IgG.

Pricing

$240/1 mg $470/2x1 mg $685/3x1 mg $890/4x1 mg $895/5x1 mg

What Are Secondary Antibodies?

Secondary antibodies are immunoglobulins that bind to primary antibodies, which are directly bound to the target antigen. They are conjugated with either an enzyme, fluorophore, or biotin, and they are almost always only targeting the constant region (Fc) of the primary antibody. As a result, this amplifies the signal in various assays and helps researchers detect, quantify, and visualize specific antigens in a complex biological sample.

Key Applications of Secondary Antibodies

• Western blotting for protein detection and quantification.


• ELISA (Enzyme-Linked Immunosorbent Assay) for antigen-antibody interaction studies and quantification.


• Immunohistochemistry (IHC) and immunofluorescence imaging for tissue and cell analysis.


• Flow cytometry for cell surface and intracellular marker detection.


• Immunoprecipitation (IP) and chromatin immunoprecipitation (ChIP) assays.


• Signal amplification in various immunoassays.


• Multiplex assays using fluorophore-conjugated secondary antibodies.

Advantages of Using Secondary Antibodies

1. High sensitivity

   
   

   Multiple secondary antibodies can bind to a single primary antibody and amplify the signal. This is especially useful for detecting antigens even if they are in low concentrations.

   
   



2. Increased flexibility in assay design

   
   

   Secondary antibodies come pre-conjugated with labels, such as enzymes, fluorophores, biotin, and more. This provides researchers with flexibility to use in multiple applications. They also support multiplexing for the simultaneous detection of multiple targets.

   
   



3. Cost-effective

   
   

   Since the Fc domain remains constant within the same animal class, only one type of secondary antibody is needed to bind many types of primary antibodies. This reduces the cost of labeling multiple primary antibodies.

   
   



4. Better signal-to-noise ratio

   
   

   Secondary antibodies minimize the background noise. As a result, they provide clearer and more accurate results.

   
   



5. Versatility

   
   

   A single species- and isotype-specific secondary antibody can be used with any compatible primary antibody. This can help streamline reagent inventory and reduce cost and preparation time.

   
   



6. Easier detection

   
   

   Unlike primary antibodies, secondary antibodies’ main characteristic is that they will almost always be partnered with a component that can be used for detection. This can make detection and visualization easy.

   
   

Why Buy Secondary Antibodies from AAA Biotech?

• Highly Validated: Most of our secondary antibodies are thoroughly tested to ensure they work reliably across different experiments.


• Versatile Applications: Our secondary antibodies can be used in a wide range of research techniques, including (but not limited to) immunocytochemistry (ICC), ELISA, immunofluorescence (IF), immunohistochemistry (IHC), flow cytometry (FC), immunoprecipitation (IP), and Western blotting (WB).


• Support for Rare Species: We offer secondary antibodies specifically designed for rare or less common species—something many other suppliers don’t provide.


• Affordable Prices: Our secondary antibodies are available at competitive prices to support all research budgets.


• Quick and Convenient Ordering: Simple online ordering process with responsive customer support.


• Privacy: We respect your privacy and ensure your information is protected at every step of the ordering process, and in all other communication with our team members.

FAQ

1. Can I reuse a secondary antibody?

Not recommended for critical experiments, as reusing may compromise sensitivity and specificity.

2. What is the difference between primary and secondary antibodies?

Primary antibodies bind directly to the antigen. Secondary antibodies bind to the primary and are usually labeled for detection.

3. How long do secondary antibodies last?

When stored properly (usually at –20°C or 4°C, depending on formulation), they can last for years. Always refer to the product datasheet.

4. What happens if you use too much secondary antibody?

Excess can increase background noise, leading to a poor signal-to-noise ratio. Titrate to find the optimal concentration.

5. Can secondary antibodies be monoclonal?

Yes. While many are polyclonal, monoclonal secondary antibodies are available for consistent batch-to-batch performance.

6. How to find secondary antibodies?

Use our catalog filters to search by host species, conjugate type, target species, and application.

7. How to store secondary antibodies?

Store at recommended temperatures away from light. Avoid repeated freeze-thaw cycles.