Secondary Antibodies

At AAA Biotech, we provide a vast collection of high-quality, purified, conjugated and unconjugated secondary antibodies to use in various applications, such as Western Blotting, Flow Cytometry, Cell Imaging, or CUT & Tag.

Our secondary antibodies are available with a wide range of labels—such as HRP, AP, FITC, and biotin—to match your detection system and enhance signal sensitivity. Each antibody is rigorously tested to ensure high specificity and minimal cross-reactivity, delivering reliable performance across multiple species and assay types.

Whether you're conducting qualitative imaging or quantitative analysis, AAA Biotech has the right secondary antibody to support your research with consistency and precision. Our entire catalog of secondary antibodies is available for browsing through our main website.

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Application Data (Anti-HSP20 Ab at 1:2,000 dilution; 30 ng of protein per lane; chicken polyclonal to goat IgG conjugated to HRP (AB1125) at 1/10,000 dilution;)

WB (Western Blot) (Western blot using using lysates at 30 ug per lane; 1st AbAnti-GAPDH Ab (AB0049) at 1:2,500 and 2nd AbAnti-goat conjugated to HRP (AB1125) at 1:10,000;)

Reactivity Data

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IgG, Polyclonal Secondary Antibody (Cat# AAA63204)

Full Name

Anti-Goat IgG, HRP

Reactivity

Goat

Applications

Western Blot

Purity

Epitope affinity purified

Pricing

$275/1 mg $525/2x1 mg $760/3x1 mg $985/4x1 mg $1,045/5x1 mg

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Application Data (Anti-GAPDH Ab at 1/2,500 dilution using HEK293 transfected cell lysates at 50 ug per lane; chicken polyclonal to goat IgG conjugated to DyLight 633 (AB200633) at 1/10,000 dilution;)

IF (Immunofluorescence) (Immunofluorescence in Drosophila larvaenmJ muscle 6/7 expressing mCherry in neurons (DyGlutmCherry) using 1st AbAnti-mCherry at 1/1,000 and 2nd AbAnti-goat IgY conjugated to DyLight633 at 1/500;)

Reactivity Data

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IgG, Polyclonal Secondary Antibody (Cat# AAA63212)

Full Name

Anti-Goat IgG, DyLight633

Reactivity

Goat

Applications

Immunofluorescence, Western Blot

Purity

Epitope affinity purified

Pricing

$275/0.5 mg $525/2x0.5 mg $760/3x0.5 mg $985/4x0.5 mg $1,045/5x0.5 mg

Application Data (PCNA was detected in paraffin-embedded sections of human lung cancer tissues using mouse anti- PCNA Antigen Affinity purified monoclonal antibody at 1 ug/mL. TRITC Conjugated Goat Anti-mouse IgG secondary antibody was used to detect the primary antibody at 30 ug/mL.)

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TRITC Conjugated Goat Anti-mouse IgG, Polyclonal Secondary Antibody (Cat# AAA45611)

Full Name

TRITC Conjugated Goat Anti-mouse IgG secondary antibody

Applications

Immunocytochemistry, Immunohistochemistry, Immunohistochemistry

Pricing

$245/0.5 mL $385/1 mL $745/2x1 mL $1,090/3x1 mL $1,410/4x1 mL $1,530/5x1 mL

WB (Western Blot) (Western Blot of Fluorescein conjugated Rabbit IgG Pre-Adsorbed secondary antibody. Lane 1: Rabbit IgG. Lane 2: none. Load: 50 ng per lane. none. Fluorescein Goat secondary antibody at 1:1,000 for 60 min at RT. Block: Blocking buffer for 30 min at RT. Size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG..)

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Goat anti Rabbit IgG (H + L) (FITC), Secondary Antibody (Cat# AAA75518)

Full Name

Goat anti Rabbit IgG (H + L) (FITC)

Reactivity

Rabbit IgG (H + L)

Applications

Immunofluorescence, Flow Cytometry

Purity

Goat anti Rabbit IgG (H + L) (FITC) was purified by affinity chromatography.

Pricing

$645/2 mg $1,250/2x2 mg $1,830/3x2 mg $2,375/4x2 mg $2,685/5x2 mg

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Application Data (This table displays additional reactivity among various species of serum and IgA (+) indicates antibody reactivity to the corresponding target.)

WB (Western Blot) (Western Blot of Biotin conjugated Monkey IgM (mu chain) secondary antibody. Lane 1: Monkey IgM. Lane 2: None. Load: 50 ng per lane. None. Biotin Goat secondary antibody at 1:1,000 for 60 min at RT. Block: Blocking buffer for 30 min t RT. Size: 72 kDa, 72 kDa for Monkey IgM..)

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Goat anti Monkey IgM (biotin), Secondary Antibody (Cat# AAA75529)

Full Name

Goat anti Monkey IgM (biotin)

Reactivity

Monkey

Applications

Western Blot, Immunohistochemistry

Purity

Goat anti Monkey IgM (biotin) was purified by delipidation, selective precipitation and tandem molecular sieve chromatography followed by dialysis.

Pricing

$905/1 mg $1,760/2x1 mg $2,570/3x1 mg $3,335/4x1 mg $3,835/5x1 mg

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Application Data (This table displays additional reactivity among various species of serum and IgA (+) indicates antibody reactivity to the corresponding target.)

WB (Western Blot) (Western Blot of Monkey IgG gamma antibody. Lane 1: Monkey IgG. Lane 2: None. Load: 100 ng per lane. Monkey IgG gamma antibody at 1:1000 for overnight at 4 deg C. DyLight800 Goat secondary antibody at 1:20,000 for 30 min at RT. Block: Blocking buffer for 30 min at RT. Size: 55 kDa, 55 kDa for Monkey IgG Gamma..)

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Goat anti Monkey IgG, Secondary Antibody (Cat# AAA75538)

Full Name

Goat anti Monkey IgG

Reactivity

Monkey IgG

Applications

Western Blot, Immunohistochemistry

Purity

Goat anti Monkey IgG was purified by delipidation, selective precipitation and tandem molecular sieve chromatography followed by dialysis.

Pricing

$665/1 mg $1,295/2x1 mg $1,890/3x1 mg $2,450/4x1 mg $2,770/5x1 mg

FLISA (FLISA plate was coated with purified human IgG1, IgG2, IgG3, and IgG4. Immunoglobulins were detected with serially diluted Mouse Anti-Human IgG2 Fd-FITC)

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Mouse Anti-Human IgG2 (gamma 2 chain specific), Monoclonal Secondary Antibody (Cat# AAA78730)

Full Name

Mouse Anti-Human IgG2 (Fd)-HRP

Reactivity

Human

Applications

FLISA, ELISA

Pricing

$375/1 mL $725/2x1 mL $1,060/3x1 mL $1,370/4x1 mL $1,485/5x1 mL

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IEP (Immunoelectrophoresis)

SDS-PAGE

Application Data

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IgG (H&L), Secondary Antibody (Cat# AAA79063)

Full Name

Goat anti-Mouse IgG (H&L) - Affinity Pure, Biotin Conjugate, min x w/bovine, goat, human, rabbit or rat IgG

Reactivity

Mouse

Applications

Western Blot, Immunohistochemistry

Purity

Affinity purified antibody is > 95% based on SDS-PAGE
Affinity purified using solid phase Mouse IgG.

Pricing

$280/1 mg $535/2x1 mg $780/3x1 mg $1,005/4x1 mg $1,030/5x1 mg

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Application Data

Application Data

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Rabbit Anti-Human DR5 (TRAIL-R2), Polyclonal Secondary Antibody (Cat# AAA78866)

Full Name

Rabbit Anti-Human DR5-UNLB

Reactivity

Human

Applications

Western Blot, Flow Cytometry

Purity

Affinity chromatography

Pricing

$405/0.1 mg $790/2x0.1 mg $1,145/3x0.1 mg $1,485/4x0.1 mg $1,620/5x0.1 mg

Application Data (Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3-UNLB followed by Rat Anti-Mouse IgG1-PE (AAA78872))

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IgG1, Monoclonal Secondary Antibody (Cat# AAA78872)

Full Name

Rat Anti-Mouse IgG1-BIOT

Reactivity

Mouse

Applications

Flow Cytometry

Pricing

$345/0.5 mg $665/2x0.5 mg $965/3x0.5 mg $1,255/4x0.5 mg $1,340/5x0.5 mg

What Are Secondary Antibodies?

Secondary antibodies are immunoglobulins that bind to primary antibodies, which are directly bound to the target antigen. They are conjugated with either an enzyme, fluorophore, or biotin, and they are almost always only targeting the constant region (Fc) of the primary antibody. As a result, this amplifies the signal in various assays and helps researchers detect, quantify, and visualize specific antigens in a complex biological sample.

Key Applications of Secondary Antibodies

• Western blotting for protein detection and quantification.


• ELISA (Enzyme-Linked Immunosorbent Assay) for antigen-antibody interaction studies and quantification.


• Immunohistochemistry (IHC) and immunofluorescence imaging for tissue and cell analysis.


• Flow cytometry for cell surface and intracellular marker detection.


• Immunoprecipitation (IP) and chromatin immunoprecipitation (ChIP) assays.


• Signal amplification in various immunoassays.


• Multiplex assays using fluorophore-conjugated secondary antibodies.

Advantages of Using Secondary Antibodies

1. High sensitivity

   
   

   Multiple secondary antibodies can bind to a single primary antibody and amplify the signal. This is especially useful for detecting antigens even if they are in low concentrations.

   
   



2. Increased flexibility in assay design

   
   

   Secondary antibodies come pre-conjugated with labels, such as enzymes, fluorophores, biotin, and more. This provides researchers with flexibility to use in multiple applications. They also support multiplexing for the simultaneous detection of multiple targets.

   
   



3. Cost-effective

   
   

   Since the Fc domain remains constant within the same animal class, only one type of secondary antibody is needed to bind many types of primary antibodies. This reduces the cost of labeling multiple primary antibodies.

   
   



4. Better signal-to-noise ratio

   
   

   Secondary antibodies minimize the background noise. As a result, they provide clearer and more accurate results.

   
   



5. Versatility

   
   

   A single species- and isotype-specific secondary antibody can be used with any compatible primary antibody. This can help streamline reagent inventory and reduce cost and preparation time.

   
   



6. Easier detection

   
   

   Unlike primary antibodies, secondary antibodies’ main characteristic is that they will almost always be partnered with a component that can be used for detection. This can make detection and visualization easy.

   
   

Why Buy Secondary Antibodies from AAA Biotech?

• Highly Validated: Most of our secondary antibodies are thoroughly tested to ensure they work reliably across different experiments.


• Versatile Applications: Our secondary antibodies can be used in a wide range of research techniques, including (but not limited to) immunocytochemistry (ICC), ELISA, immunofluorescence (IF), immunohistochemistry (IHC), flow cytometry (FC), immunoprecipitation (IP), and Western blotting (WB).


• Support for Rare Species: We offer secondary antibodies specifically designed for rare or less common species—something many other suppliers don’t provide.


• Affordable Prices: Our secondary antibodies are available at competitive prices to support all research budgets.


• Quick and Convenient Ordering: Simple online ordering process with responsive customer support.


• Privacy: We respect your privacy and ensure your information is protected at every step of the ordering process, and in all other communication with our team members.

FAQ

1. Can I reuse a secondary antibody?

Not recommended for critical experiments, as reusing may compromise sensitivity and specificity.

2. What is the difference between primary and secondary antibodies?

Primary antibodies bind directly to the antigen. Secondary antibodies bind to the primary and are usually labeled for detection.

3. How long do secondary antibodies last?

When stored properly (usually at –20°C or 4°C, depending on formulation), they can last for years. Always refer to the product datasheet.

4. What happens if you use too much secondary antibody?

Excess can increase background noise, leading to a poor signal-to-noise ratio. Titrate to find the optimal concentration.

5. Can secondary antibodies be monoclonal?

Yes. While many are polyclonal, monoclonal secondary antibodies are available for consistent batch-to-batch performance.

6. How to find secondary antibodies?

Use our catalog filters to search by host species, conjugate type, target species, and application.

7. How to store secondary antibodies?

Store at recommended temperatures away from light. Avoid repeated freeze-thaw cycles.