Secondary Antibodies

At AAA Biotech, we provide a vast collection of high-quality, purified, conjugated and unconjugated secondary antibodies to use in various applications, such as Western Blotting, Flow Cytometry, Cell Imaging, or CUT & Tag.

Our secondary antibodies are available with a wide range of labels—such as HRP, AP, FITC, and biotin—to match your detection system and enhance signal sensitivity. Each antibody is rigorously tested to ensure high specificity and minimal cross-reactivity, delivering reliable performance across multiple species and assay types.

Whether you're conducting qualitative imaging or quantitative analysis, AAA Biotech has the right secondary antibody to support your research with consistency and precision. Our entire catalog of secondary antibodies is available for browsing through our main website.

ELISA (ELISA plate was coated with purified human IgG, IgM, IgA, and IgE. Immunoglobulins were detected with serially diluted Mouse Anti-Human IgE Fc-BIOT followed by Streptavidin-HRP)

Compare (5 max)

Mouse Anti-Human IgE (epsilon chain specific), Monoclonal Secondary Antibody (Cat# AAA78748)

Full Name

Mouse Anti-Human IgE (Fc)-AP

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoblot

Pricing

$375/1 mL $725/2x1 mL $1,060/3x1 mL $1,370/4x1 mL $1,485/5x1 mL

Application Data (Human pancreatic carcinoma cell line MIA PaCa-2 was stainied with Mouse-Anti-Cytokeratin 18-UNLB ; right) followed by Rat anti-mouse IgG2b-BIOT , streptavidin-CY3)

Compare (5 max)

IgG2b, Monoclonal Secondary Antibody (Cat# AAA78870)

Full Name

Rat Anti-Mouse IgG2b-UNLB

Applications

Western Blot, Immunocytochemistry, Flow Cytometry

Pricing

$320/0.5 mg $615/2x0.5 mg $895/3x0.5 mg $1,160/4x0.5 mg $1,225/5x0.5 mg

Application Data

Compare (5 max)

Goat Anti-Human lambda (lambda chain specific), Polyclonal Secondary Antibody (Cat# AAA78804)

Full Name

Goat Anti-Human Lambda-UNLB

Reactivity

Human

Applications

Flow Cytometry

Purity

Affinity chromatography on human lambda light chains covalently linked to agarose

Pricing

$250/1 mg $490/2x1 mg $715/3x1 mg $930/4x1 mg $935/5x1 mg

Application Data (PCK was detected in paraffin-embedded sections of human intestinal cancer tissues using mouse anti- PCK Antigen Affinity purified monoclonal antibody at 1 ug/mL. FITC Conjugated Protein A was used to detect the primary antibody at 30 ug/mL.)

Compare (5 max)

FITC Conjugated Protein A, Secondary Antibody (Cat# AAA45598)

Full Name

FITC Conjugated Protein A

Applications

Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Flow Cytometry

Pricing

$245/0.5 mL $360/1 mL $695/2x1 mL $1,010/3x1 mL $1,315/4x1 mL $1,405/5x1 mL

< >

<< Previous Next >>

Application Data (PCK was detected in paraffin-embedded sections of human intestinal cancer tissues using mouse anti- PCK Antigen Affinity purified monoclonal antibody at 1 ug/mL. TRITC Conjugated Avidin was used for visualization at 20ug/mL.)

Application Data (Beta Catenin was detected in paraffin-embedded sections of human intestinal cancer tissues using rabbit anti-Beta Catenin Antigen Affinity purified polyclonal antibody at 1 ug/mL. TRITC Conjugated Avidin was used for visualization at 20 ug/mL.)

Compare (5 max)

TRITC Conjugated Avidin, Secondary Antibody (Cat# AAA45599)

Full Name

TRITC Conjugated Avidin

Applications

Immunocytochemistry, Immunohistochemistry, Immunohistochemistry

Pricing

$245/0.5 mL $385/1 mL $745/2x1 mL $1,090/3x1 mL $1,410/4x1 mL $1,530/5x1 mL

< >

<< Previous Next >>

FCM/FACS (Flow Cytometry) (Flow cytometry: 1X10^6 K-562 cells (negative control,left) and A-431 cells (right) were surface-stained with Purified Rabbit anti-Human E-Cadherin mAb(5 ul/Test,orange line) or secondary antibody only (blue line). Non-fluorescently stained K-562 and A-431 cells were used as blank control (red line). FITC Goat Anti-Rabbit IgG (H+L)(AAA37342, 1:200) was used as a secondary antibody)

IF (Immunofluorescence) (Immunofluorescence analysis of HepG2 cells , using ACLY antibody (AAA37342) as the primary antibody at dilution of 1:100. Secondary antibody: FITC Goat Anti-Rabbit IgG (H+L) AAA37342 ) . Blue: DAPI for nuclear staining.)

Compare (5 max)

IgG (H+L), Secondary Antibody (Cat# AAA37342)

Full Name

FITC Goat Anti-Rabbit IgG (H+L) Antibody

Applications

Immunocytochemistry, Flow Cytometry, Immunofluorescence

Purity

Affinity Purification

Pricing

$170/0.1 mL $330/2x0.1 mL $480/3x0.1 mL $535/5x0.1 mL $620/4x0.1 mL

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (Collagen IV was detected in paraffin-embedded sections of rat kidney tissues using rabbit anti- Collagen IV Antigen Affinity purified polyclonal antibody at 1 ug/mL. Cy3 Conjugated Goat Anti-Rabbit IgG secondary antibody was used to detect the primary antibody at 20ug/mL.)

IHC (Immunohistochemistry) (MCM2 was detected in paraffin-embedded sections of human intestinal cancer tissues using rabbit anti- MCM2 Antigen Affinity purified polyclonal antibody at 1ug/mL. Cy3 Conjugated Goat Anti-Rabbit IgG secondary antibody was used to detect the primary antibody at 20ug/mL.)

Compare (5 max)

Cy3 Conjugated Goat Anti-Rabbit IgG, Polyclonal Secondary Antibody (Cat# AAA45614)

Full Name

Cy3 Conjugated Goat Anti-Rabbit IgG secondary antibody

Applications

Immunocytochemistry, Immunohistochemistry, Immunohistochemistry

Pricing

$245/0.5 mL $480/2x0.5 mL $700/3x0.5 mL $910/4x0.5 mL $910/5x0.5 mL

FCM/FACS (Flow Cytometry) (Human peripheral blood lymphocytes are stained with Anti-Human IgM Monoclonal Antibody(PE/Cyanine5 Conjugated)(filled gray histogram) or Mouse IgG1 Isotype Control PE/Cy5 (empty black histogram).)

Compare (5 max)

IgM, Monoclonal Secondary Antibody (Cat# AAA174756)

Full Name

Anti-Human IgM Monoclonal Antibody (PE/Cyanine5 Conjugated) [MHM-88]

Reactivity

Human

Applications

Flow Cytometry

Pricing

$170/20 Tests $300/100 Tests $370/2x100 Tests $1,645/10x100 Tests

WB (Western Blot) (Western blot of FITC conjugated Goat F(ab')2 Anti-Human IgG F(ab')2. Lane 1: Human Fab2. Lane 2: None. Load: 50 ng per lane. None. FITC Goat secondary antibody at 1:1,000 for 60 min at RT. Blocking: Blocking buffer for 30 min at RT. Size: 25 kDa, 25 kDa for Human IgG F(ab')2..)

Compare (5 max)

Goat anti Human IgG (Fab'2) (FITC), Secondary Antibody (Cat# AAA75498)

Full Name

Goat anti Human IgG (Fab'2) (FITC)

Reactivity

To be determined by end-user

Applications

Immunofluorescence, Flow Cytometry

Purity

Goat anti Human IgG (Fab'2) (FITC) was purified by affinity chromatography.

Pricing

$835/1 mg $1,620/2x1 mg $2,365/3x1 mg $3,070/4x1 mg $3,510/5x1 mg

IF (Immunofluorescence)

Compare (5 max)

Rat Anti-Mouse Kappa (kappa chain specific), Monoclonal Secondary Antibody (Cat# AAA78651)

Full Name

Rat Anti-Mouse Kappa-UNLB

Reactivity

Mouse

Applications

Immunofluorescence, Flow Cytometry

Pricing

$310/0.5 mg $595/2x0.5 mg $865/3x0.5 mg $1,120/4x0.5 mg $1,180/5x0.5 mg

< >

<< Previous Next >>

Application Data

Application Data

Compare (5 max)

IgG (H&L), Secondary Antibody (Cat# AAA60804)

Full Name

DyLight 800 Conjugated Affinity Purified anti-Mouse IgG (H&L) [Goat]

Reactivity

Mouse

Applications

Immunofluorescence

Pricing

$405/0.1 mg $790/2x0.1 mg $1,145/3x0.1 mg $1,485/4x0.1 mg $1,500/5x0.1 mg

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (PCNA was detected in paraffin-embedded sections of human intestinal cancer tissues using mouse anti- PCNA Antigen Affinity purified monoclonal antibody at 1 ug/mL. Cy3 Conjugated Goat Anti-Mouse IgG secondary antibody was used to detect the primary antibody at 20ug/mL.)

IHC (Immunohistochemistry) (PCK was detected in paraffin-embedded sections of human intestinal cancer tissues using mouse anti- PCK Antigen Affinity purified monoclonal antibody at 1ug/mL. Cy3 Conjugated Goat Anti-Mouse IgG secondary antibody was used to detect the primary antibody at 20ug/mL.)

Compare (5 max)

Cy3 Conjugated Goat Anti-Mouse IgG, Polyclonal Secondary Antibody (Cat# AAA45618)

Full Name

Cy3 Conjugated Goat Anti-Mouse IgG secondary antibody

Applications

Immunocytochemistry, Immunohistochemistry, Immunohistochemistry

Pricing

$245/0.5 mL $480/2x0.5 mL $700/3x0.5 mL $910/4x0.5 mL $910/5x0.5 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Figure 2. IF analysis of COX5B using anti-COX5B antibody (A06090-2).COX5B was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5ug/mL rabbit anti-COX5B Antibody (A06090-2) overnight at 4 degree C. DyLight 647 Conjugated Goat Anti-Rabbit IgG (AAA127948) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

WB (Western Blot) (Figure 1. Western blot analysis of APEX1 using anti-APEX1 antibody (PB9128).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human K562 whole cell lysates,Lane 2: human HepG2 whole cell lysates,Lane 3: human Raji whole cell lysates,Lane 4: human A431 whole cell lysates,Lane 5: human A549 whole cell lysates,Lane 6: human MCF-7 whole cell lysates,Lane 7: human RT4 whole cell lysates,Lane 8: human U87 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APEX1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for APEX1 at approximately 36 kDa. The expected band size for APEX1 is at 36 kDa.)

Compare (5 max)

IgG, Polyclonal Secondary Antibody (Cat# AAA127948)

Full Name

DyLight 647 Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)

Reactivity

Rabbit

Applications

Western Blot, Immunofluorescence, Flow Cytometry

Pricing

$180/0.1 mL $350/2x0.1 mL $505/3x0.1 mL $620/5x0.1 mL $660/4x0.1 mL

WB (Western Blot)

Compare (5 max)

IgG, Secondary Antibody (Cat# AAA62290)

Full Name

Goat Anti-Rabbit & Mouse IgG-HRP

Reactivity

Rabbit

Applications

Dot Blot, Western Blot

Pricing

$215/0.1 mL $390/1 mL $1,290/5 mL $2,510/2x5 mL $3,670/3x5 mL $4,760/4x5 mL $5,460/5x5 mL

What Are Secondary Antibodies?

Secondary antibodies are immunoglobulins that bind to primary antibodies, which are directly bound to the target antigen. They are conjugated with either an enzyme, fluorophore, or biotin, and they are almost always only targeting the constant region (Fc) of the primary antibody. As a result, this amplifies the signal in various assays and helps researchers detect, quantify, and visualize specific antigens in a complex biological sample.

Key Applications of Secondary Antibodies

• Western blotting for protein detection and quantification.


• ELISA (Enzyme-Linked Immunosorbent Assay) for antigen-antibody interaction studies and quantification.


• Immunohistochemistry (IHC) and immunofluorescence imaging for tissue and cell analysis.


• Flow cytometry for cell surface and intracellular marker detection.


• Immunoprecipitation (IP) and chromatin immunoprecipitation (ChIP) assays.


• Signal amplification in various immunoassays.


• Multiplex assays using fluorophore-conjugated secondary antibodies.

Advantages of Using Secondary Antibodies

1. High sensitivity

   
   

   Multiple secondary antibodies can bind to a single primary antibody and amplify the signal. This is especially useful for detecting antigens even if they are in low concentrations.

   
   



2. Increased flexibility in assay design

   
   

   Secondary antibodies come pre-conjugated with labels, such as enzymes, fluorophores, biotin, and more. This provides researchers with flexibility to use in multiple applications. They also support multiplexing for the simultaneous detection of multiple targets.

   
   



3. Cost-effective

   
   

   Since the Fc domain remains constant within the same animal class, only one type of secondary antibody is needed to bind many types of primary antibodies. This reduces the cost of labeling multiple primary antibodies.

   
   



4. Better signal-to-noise ratio

   
   

   Secondary antibodies minimize the background noise. As a result, they provide clearer and more accurate results.

   
   



5. Versatility

   
   

   A single species- and isotype-specific secondary antibody can be used with any compatible primary antibody. This can help streamline reagent inventory and reduce cost and preparation time.

   
   



6. Easier detection

   
   

   Unlike primary antibodies, secondary antibodies’ main characteristic is that they will almost always be partnered with a component that can be used for detection. This can make detection and visualization easy.

   
   

Why Buy Secondary Antibodies from AAA Biotech?

• Highly Validated: Most of our secondary antibodies are thoroughly tested to ensure they work reliably across different experiments.


• Versatile Applications: Our secondary antibodies can be used in a wide range of research techniques, including (but not limited to) immunocytochemistry (ICC), ELISA, immunofluorescence (IF), immunohistochemistry (IHC), flow cytometry (FC), immunoprecipitation (IP), and Western blotting (WB).


• Support for Rare Species: We offer secondary antibodies specifically designed for rare or less common species—something many other suppliers don’t provide.


• Affordable Prices: Our secondary antibodies are available at competitive prices to support all research budgets.


• Quick and Convenient Ordering: Simple online ordering process with responsive customer support.


• Privacy: We respect your privacy and ensure your information is protected at every step of the ordering process, and in all other communication with our team members.

FAQ

1. Can I reuse a secondary antibody?

Not recommended for critical experiments, as reusing may compromise sensitivity and specificity.

2. What is the difference between primary and secondary antibodies?

Primary antibodies bind directly to the antigen. Secondary antibodies bind to the primary and are usually labeled for detection.

3. How long do secondary antibodies last?

When stored properly (usually at –20°C or 4°C, depending on formulation), they can last for years. Always refer to the product datasheet.

4. What happens if you use too much secondary antibody?

Excess can increase background noise, leading to a poor signal-to-noise ratio. Titrate to find the optimal concentration.

5. Can secondary antibodies be monoclonal?

Yes. While many are polyclonal, monoclonal secondary antibodies are available for consistent batch-to-batch performance.

6. How to find secondary antibodies?

Use our catalog filters to search by host species, conjugate type, target species, and application.

7. How to store secondary antibodies?

Store at recommended temperatures away from light. Avoid repeated freeze-thaw cycles.