Secondary Antibodies

At AAA Biotech, we provide a vast collection of high-quality, purified, conjugated and unconjugated secondary antibodies to use in various applications, such as Western Blotting, Flow Cytometry, Cell Imaging, or CUT & Tag.

Our secondary antibodies are available with a wide range of labels—such as HRP, AP, FITC, and biotin—to match your detection system and enhance signal sensitivity. Each antibody is rigorously tested to ensure high specificity and minimal cross-reactivity, delivering reliable performance across multiple species and assay types.

Whether you're conducting qualitative imaging or quantitative analysis, AAA Biotech has the right secondary antibody to support your research with consistency and precision. Our entire catalog of secondary antibodies is available for browsing through our main website.

Application Data (ELISA plate was coated with purified rat IgM and IgG. Immunoglobins were detected with serially diluted Goat Anti-Rat IgM-AP)

Compare (5 max)

Goat Anti-Rat IgM (u chain specific), Polyclonal Secondary Antibody (Cat# AAA78809)

Full Name

Goat Anti-Rat IgM-AP

Reactivity

Rat IgG; may react with immunoglobins from other species

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunoblot

Purity

Affinity chromatography on pooled rat IgM covalently linked to agarose

Pricing

$305/1 mL $585/2x1 mL $850/3x1 mL $1,105/4x1 mL $1,160/5x1 mL

Application Data (FLISA plate was coated with purified feline IgG. Immunoglobulin was detected with Goat Anti-Feline IgG(H+L)-FITC)

Compare (5 max)

Goat Anti-Feline IgG (H+L), Polyclonal Secondary Antibody (Cat# AAA78835)

Full Name

Goat Anti-Feline IgG (H+L)-HRP

Reactivity

Feline

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunoblot

Purity

Affinity chromatography on feline IgG covalently linked to agarose.

Pricing

$205/1 mL $400/2x1 mL $585/3x1 mL $730/5x1 mL $755/4x1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot of Texas Red Conjugated Rabbit IgG secondary antibody. Lane 1: Rabbit IgG. Lane 2: None. Load: 50 ng per lane. None. Texas Red Goat secondary antibody at 1:1,000 for 60 min at RT. Blocking: Blocking buffer for 30 min at RT. Size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG..)

DB (Dot Blot) (Dot Blot showing the detection of Rabbit IgG. A three-fold serial dilution of Rabbit IgG starting at 200ng was spotted onto nitrocellulose. After blocking in 5% blocking buffer for 1 Hour at 20 deg C, Goat anti Rabbit IgG (H&L) Texas Red Conjugate was used at 1:1000 in Blocking Buffer.)

Compare (5 max)

Goat anti Rabbit IgG (H + L) (Texas Red), Secondary Antibody (Cat# AAA75485)

Full Name

Goat anti Rabbit IgG (H + L) (Texas Red)

Reactivity

Rabbit IgG (H + L)

Applications

Immunofluorescence, Flow Cytometry

Purity

Goat anti Rabbit IgG (H + L) was purified by affinity chromatography.

Pricing

$560/2 mg $1,080/2x2 mg $1,580/3x2 mg $2,050/4x2 mg $2,270/5x2 mg

WB (Western Blot) (Western blot of Texas Red conjugated Mouse IgG1 (Gamma 1 chain) secondary antibody. Lane 1: Mouse IgG1. Lane 2: None. Load: 50 ng per lane. None. Texas Red rabbit secondary antibody at 1:1,000 for 60 min at RT. Blocking: Blocking buffer for 30 min at RT. Size: 55 kDa, 55 kDa for Mouse IgG1 (Gamma 1 chain)..)

Compare (5 max)

Rabbit anti Mouse IgG1 (Texas Red), Secondary Antibody (Cat# AAA75492)

Full Name

Rabbit anti Mouse IgG1 (Texas Red)

Reactivity

Murine IgG1

Applications

Immunofluorescence, Flow Cytometry

Purity

Rabbit anti Mouse IgG1 was purified by affinity chromatography.

Pricing

$800/1 mg $1,550/2x1 mg $2,255/3x1 mg $2,930/4x1 mg $3,360/5x1 mg

< >

<< Previous Next >>

DB (Dot Blot) (Dot Blot of Mouse IgG1 Secondary Antibody Alkaline Phosphatase Conjugated. Antigen: Mouse IgG1. Load: Lane 1 - 200ng Lane 2 - 66.7ng Lane 3 - 22.2ng Lane 4 - 7.4ng Lane 5 - 2.5ng. Primary antibody: none. Secondary antibody: Mouse IgG1 Secondary Antibody Alkaline Phosphatase Conjugated at 1:1,000 for one hour at RT. Block: MB-070 for 60 min at RT. Reaction visualized using alkaline phosphatase substrate for 30 seconds at RT.)

WB (Western Blot) (Western Blot of Mouse IgG1 Secondary antibody Alkaline Phosphatase Conjugated. Lane 1: Mouse IgG1. Lane 2: none. Load: 50ng per lane. none. Mouse IgG1 Secondary antibody Alkaline Phosphatase Conjugated at 1:1,000 o/n at 4 deg C. Block: Blocking buffer for 30 min at RT. Reaction visualized using alkaline phosphatase substrate for 30 seconds. Size: 55 kDa for Mouse IgG1.)

Compare (5 max)

Rabbit anti Mouse IgG1 (Alk Phos), Secondary Antibody (Cat# AAA75493)

Full Name

Rabbit anti Mouse IgG1 (Alk Phos)

Reactivity

Murine IgG1

Applications

Western Blot, Immunohistochemistry

Purity

Rabbit anti Mouse IgG1 (Alk Phos) was purified by affinity chromatography.

Pricing

$965/1 mg $1,880/2x1 mg $2,745/3x1 mg $3,565/4x1 mg $4,115/5x1 mg

Application Data (Rabbit peripheral bllod lymphocytes were stained with Rat Anti- Rabbit Ig-BIOT followed by Streptavidin-FITC .)

Compare (5 max)

Rat Anti-Rabbit Ig, Monoclonal Secondary Antibody (Cat# AAA78699)

Full Name

Rat Anti-Rabbit Ig-HRP

Reactivity

Minimal reactivity to human, mouse, rat, hamster, goat, sheep, porcine, canine and rhesus IgG and guinea pig serum.

Applications

Flow Cytometry

Pricing

$345/1 mL $665/2x1 mL $965/3x1 mL $1,255/4x1 mL $1,340/5x1 mL

Standard Curve (Sample)

Compare (5 max)

Mouse Anti-Human IgG (Fc) (gamma chain specific), Monoclonal Secondary Antibody (Cat# AAA78718)

Full Name

Mouse Anti-Human IgG (Fc)-UNLB

Reactivity

Human

Applications

Flow Cytometry

Pricing

$280/0.5 mg $535/2x0.5 mg $780/3x0.5 mg $1,005/4x0.5 mg $1,045/5x0.5 mg

ELISA (ELISA plate was coated with purified human IgG1, IgG2, IgG3, and IgG4. Immunoglobulins were detected with serially diluted Mouse Anti-Human IgG2 Fc-HRP.)

Compare (5 max)

Mouse Anti-Human IgG2 (gamma 2 chain specific), Monoclonal Secondary Antibody (Cat# AAA78725)

Full Name

Mouse Anti-Human IgG2 (Fc)-HRP

Reactivity

Human

Applications

FLISA, ELISA

Pricing

$355/1 mL $685/2x1 mL $995/3x1 mL $1,295/4x1 mL $1,380/5x1 mL

Application Data (FLISA plate was coated with purified human IgG1, IgG2, IgG3, and IgG4. Immunoglobulins were detected with serially diluted Mouse Anti-human IgG4 Fc-PE)

Compare (5 max)

Mouse Anti-Human IgG4 (gamma 4 chain specific), Monoclonal Secondary Antibody (Cat# AAA78737)

Full Name

Mouse Anti-Human IgG4 (Fc)-BIOT

Reactivity

Human

Pricing

$335/0.5 mg $645/2x0.5 mg $940/3x0.5 mg $1,215/4x0.5 mg $1,290/5x0.5 mg

< >

<< Previous Next >>

SDS-PAGE

Application Data

Compare (5 max)

IgG (H&L), F(ab)'2 Fragment, Secondary Antibody (Cat# AAA79065)

Full Name

Goat anti-Mouse IgG (H&L), F(ab)'2 Fragment - Affinity Pure, Biotin Conjugate, min x w/human IgG or serum proteins

Reactivity

Mouse

Applications

Flow Cytometry, Western Blot, Immunohistochemistry

Purity

> 90% based on SDS-PAGE Small amounts of intact IgG may be present.
Affinity purified using solid phase Mouse IgG.

Pricing

$290/0.5 mg $555/2x0.5 mg $805/3x0.5 mg $1,045/4x0.5 mg $1,085/5x0.5 mg

< >

<< Previous Next >>

SDS-PAGE

Application Data (IEP (Immunoelectophorosis))

Compare (5 max)

IgG (H&L), Secondary Antibody (Cat# AAA79067)

Full Name

Sheep anti-Rabbit IgG (H&L) - Affinity Pure, HRP Conjugate

Reactivity

Rabbit.
Cross Reactivity: Based on IEP, no reactivity is observed to non-immunoglobulin rabbit serum proteins

Applications

Immunohistochemistry, Western Blot

Purity

Affinity purified antibody is > 95% based on SDS-PAGE
Affinity purified using solid phase Rabbit IgG.

Pricing

$235/1 mg $460/2x1 mg $670/3x1 mg $870/5x1 mg $870/4x1 mg

Application Data

Compare (5 max)

IgG, Secondary Antibody (Cat# AAA79081)

Full Name

Goat anti-Human IgG, secondary antibody, Conjugated to Europium 1024

Reactivity

Human

Pricing

$660/0.05 mg $1,285/2x0.05 mg $1,875/3x0.05 mg $2,430/4x0.05 mg $2,775/5x0.05 mg

Application Data (ELISA plate was coated with purified human IgG1, IgG2, IgG3, and IgG4. Immunoglobulins were detected with serially diluted Mouse Anti-Human IgG1 Fc-AP (SB Cat. No. AAA78634).)

Compare (5 max)

Mouse Anti-Human IgG1 Fc, Secondary Antibody (Cat# AAA78634)

Full Name

Mouse Anti-Human IgG1 Fc-AP

Applications

ELISA

Pricing

$320/1 mL $615/2x1 mL $895/3x1 mL $1,160/4x1 mL $1,225/5x1 mL

FLISA (FLISA plate was coated with purified human IgG, IgM, IgA, and IgE. Immunoglobulins were detected with serially diluted Mouse Anti-Human IgE Fc-PE)

Compare (5 max)

IgE, Monoclonal Secondary Antibody (Cat# AAA78932)

Full Name

Mouse Anti-Human IgE Fc-PE

Gene Names

IGHE; IgE

Reactivity

Human

Applications

FLISA

Pricing

$375/0.1 mg $725/2x0.1 mg $1,060/3x0.1 mg $1,370/4x0.1 mg $1,485/5x0.1 mg

Application Data (FLISA plate was coated with purified mouse IgE, IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA.Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgE-FITC .)

Compare (5 max)

Goat Anti-Mouse IgE, Polyclonal Secondary Antibody (Cat# AAA78785)

Full Name

Goat Anti-Mouse IgE-HRP

Reactivity

Mouse IgG₁, IgG_2a, IgG₃, IgM, and IgA; may react with immunoglobulins from other species

Applications

FLISA, ELISA

Purity

Affinity chromatography on mouse IgE covalently linked to agarose

Pricing

$340/1 mL $655/2x1 mL $955/3x1 mL $1,235/4x1 mL $1,315/5x1 mL

Application Data (Total cell lysates from Jurkat cells were resolved by electrophoresis, transferred to PVDF membrane. and probed with Rabbit Anti-Human DR5~UNLa (Cat. Proteins were visualized using Goat Anti-Rabbit IgG(H+L), Mouse/Human ads-HRP (Cat. secondary antibody and chemllumfnescent detection.)

Compare (5 max)

Goat Anti-Rabbit IgG (H+L chain specific), Secondary Antibody (Cat# AAA78824)

Full Name

Goat Anti-Rabbit IgG (H+L), Mouse/Human ads-TXRD

Reactivity

Rabbit

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry, Immunohistochemistry, Flow Cytometry

Purity

Affinity chromatography on pooled IgG covalently linked to agarose.

Pricing

$210/1 mg $410/2x1 mg $600/3x1 mg $760/5x1 mg $775/4x1 mg

Application Data (ELISA plate was coated with purified bovine IgG. Immunoglobulin was detected with Goat Anti-Bovine IgG(H+L))

Compare (5 max)

Goat Anti-Bovine IgG (H+L), Polyclonal Secondary Antibody (Cat# AAA78829)

Full Name

Goat Anti-Bovine IgG (H+L)-UNLB

Reactivity

Bovine

Applications

FLISA, ELISA

Purity

Affinity chromatography on bovine IgG covalently linked to agarose.

Pricing

$175/1 mg $340/2x1 mg $490/3x1 mg $585/5x1 mg $640/4x1 mg

What Are Secondary Antibodies?

Secondary antibodies are immunoglobulins that bind to primary antibodies, which are directly bound to the target antigen. They are conjugated with either an enzyme, fluorophore, or biotin, and they are almost always only targeting the constant region (Fc) of the primary antibody. As a result, this amplifies the signal in various assays and helps researchers detect, quantify, and visualize specific antigens in a complex biological sample.

Key Applications of Secondary Antibodies

• Western blotting for protein detection and quantification.


• ELISA (Enzyme-Linked Immunosorbent Assay) for antigen-antibody interaction studies and quantification.


• Immunohistochemistry (IHC) and immunofluorescence imaging for tissue and cell analysis.


• Flow cytometry for cell surface and intracellular marker detection.


• Immunoprecipitation (IP) and chromatin immunoprecipitation (ChIP) assays.


• Signal amplification in various immunoassays.


• Multiplex assays using fluorophore-conjugated secondary antibodies.

Advantages of Using Secondary Antibodies

1. High sensitivity

   
   

   Multiple secondary antibodies can bind to a single primary antibody and amplify the signal. This is especially useful for detecting antigens even if they are in low concentrations.

   
   



2. Increased flexibility in assay design

   
   

   Secondary antibodies come pre-conjugated with labels, such as enzymes, fluorophores, biotin, and more. This provides researchers with flexibility to use in multiple applications. They also support multiplexing for the simultaneous detection of multiple targets.

   
   



3. Cost-effective

   
   

   Since the Fc domain remains constant within the same animal class, only one type of secondary antibody is needed to bind many types of primary antibodies. This reduces the cost of labeling multiple primary antibodies.

   
   



4. Better signal-to-noise ratio

   
   

   Secondary antibodies minimize the background noise. As a result, they provide clearer and more accurate results.

   
   



5. Versatility

   
   

   A single species- and isotype-specific secondary antibody can be used with any compatible primary antibody. This can help streamline reagent inventory and reduce cost and preparation time.

   
   



6. Easier detection

   
   

   Unlike primary antibodies, secondary antibodies’ main characteristic is that they will almost always be partnered with a component that can be used for detection. This can make detection and visualization easy.

   
   

Why Buy Secondary Antibodies from AAA Biotech?

• Highly Validated: Most of our secondary antibodies are thoroughly tested to ensure they work reliably across different experiments.


• Versatile Applications: Our secondary antibodies can be used in a wide range of research techniques, including (but not limited to) immunocytochemistry (ICC), ELISA, immunofluorescence (IF), immunohistochemistry (IHC), flow cytometry (FC), immunoprecipitation (IP), and Western blotting (WB).


• Support for Rare Species: We offer secondary antibodies specifically designed for rare or less common species—something many other suppliers don’t provide.


• Affordable Prices: Our secondary antibodies are available at competitive prices to support all research budgets.


• Quick and Convenient Ordering: Simple online ordering process with responsive customer support.


• Privacy: We respect your privacy and ensure your information is protected at every step of the ordering process, and in all other communication with our team members.

FAQ

1. Can I reuse a secondary antibody?

Not recommended for critical experiments, as reusing may compromise sensitivity and specificity.

2. What is the difference between primary and secondary antibodies?

Primary antibodies bind directly to the antigen. Secondary antibodies bind to the primary and are usually labeled for detection.

3. How long do secondary antibodies last?

When stored properly (usually at –20°C or 4°C, depending on formulation), they can last for years. Always refer to the product datasheet.

4. What happens if you use too much secondary antibody?

Excess can increase background noise, leading to a poor signal-to-noise ratio. Titrate to find the optimal concentration.

5. Can secondary antibodies be monoclonal?

Yes. While many are polyclonal, monoclonal secondary antibodies are available for consistent batch-to-batch performance.

6. How to find secondary antibodies?

Use our catalog filters to search by host species, conjugate type, target species, and application.

7. How to store secondary antibodies?

Store at recommended temperatures away from light. Avoid repeated freeze-thaw cycles.