Secondary Antibodies

At AAA Biotech, we provide a vast collection of high-quality, purified, conjugated and unconjugated secondary antibodies to use in various applications, such as Western Blotting, Flow Cytometry, Cell Imaging, or CUT & Tag.

Our secondary antibodies are available with a wide range of labels—such as HRP, AP, FITC, and biotin—to match your detection system and enhance signal sensitivity. Each antibody is rigorously tested to ensure high specificity and minimal cross-reactivity, delivering reliable performance across multiple species and assay types.

Whether you're conducting qualitative imaging or quantitative analysis, AAA Biotech has the right secondary antibody to support your research with consistency and precision. Our entire catalog of secondary antibodies is available for browsing through our main website.

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (Ki67 was detected in paraffin-embedded sections of human intestinal cancer tissues using rabbit anti- Ki67 Antigen Affinity purified polyclonal antibody at 1 ug/mL. Cy3 Conjugated Avidin was used for visualization at 10ug/mL.)

IHC (Immunohistochemistry) (Beta Catenin was detected in paraffin-embedded sections of human intestinal cancer tissues using rabbit anti-Beta Catenin Antigen Affinity purified polyclonal antibody at 1ug/mL. Cy3 Conjugated Avidin was used for visualization at 10ug/mL.)

Compare (5 max)

Cy3 Conjugated Avidin, Polyclonal Secondary Antibody (Cat# AAA45606)

Full Name

Cy3 Conjugated Avidin

Applications

Immunofluorescence, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry

Pricing

$225/0.5 mL $440/2x0.5 mL $645/3x0.5 mL $820/5x0.5 mL $835/4x0.5 mL

DB (Dot Blot) (Dot Blot of Goat anti-Bovine IgG Fab2 Antibody Fluorescein Conjugated. Antigen: Bovine IgG. Load: Lane 1 - 100 ng Lane 2 - 33.3 ng Lane 3 - 11.1 ng Lane 4 - 3.70 ng Lane 5 - 1.23 ng. Primary antibody: n/a. Secondary antibody: Goat anti-Bovine IgG Fab2 Antibody Fluorescein Conjugated at 1:1,000 for 1 HR at RT. Block: MB-070 for 1 HR at RT.)

Compare (5 max)

Goat anti Bovine IgG (FITC), Secondary Antibody (Cat# AAA75476)

Full Name

Goat anti Bovine IgG (FITC)

Reactivity

Bovine IgG

Applications

Immunofluorescence, Flow Cytometry

Purity

Goat anti Bovine IgG (FITC) was purified by delipidation, salt fractionation and ion exchange chromatography followed by dialysis.

Pricing

$620/2 mg $1,200/2x2 mg $1,755/3x2 mg $2,275/4x2 mg $2,565/5x2 mg

< >

<< Previous Next >>

IEP (Immunoelectrophoresis)

SDS-PAGE

Application Data

Compare (5 max)

IgG (H&L), F(ab)'2 fragment, Secondary Antibody (Cat# AAA79070)

Full Name

Donkey anti-Mouse IgG (H&L) - F(ab)'2 fragment, Biotin Conjugate, min x w/bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep IgG

Reactivity

Mouse

Applications

Flow Cytometry, Western Blot, Immunohistochemistry

Purity

Antibody was prepared using solid phase Mouse IgG.
F(ab)'2 fragment was prepared by pepsin digestion.
Affinity purified is >90% based on SDS-PAGE

Pricing

$420/0.5 mg $820/2x0.5 mg $1,190/3x0.5 mg $1,545/4x0.5 mg $1,685/5x0.5 mg

WB (Western Blot) (Western blot of Peroxidase conjugated Rabbit IgG F(c) secondary antibody. Lane 1: Rabbit IgG F(c). Lane 2: None. Load: 50 ng per lane. None. Peroxidase Goat secondary antibody at 1:1,000 for 60 min at RT. Blocking: Blocking buffer for 30 min at RT. Size: 28 kDa, 28 kDa for Rabbit IgG F(c)..)

Compare (5 max)

Goat anti Rabbit IgG (HRP), Secondary Antibody (Cat# AAA75620)

Full Name

Goat anti Rabbit IgG (HRP)

Reactivity

Rabbit IgG

Applications

Western Blot, Immunohistochemistry

Purity

Goat anti Rabbit IgG (HRP) was purified by affinity chromatography.

Pricing

$925/2 mg $1,805/2x2 mg $2,630/3x2 mg $3,410/4x2 mg $3,945/5x2 mg

< >

<< Previous Next >>

Application Data (This table displays additional reactivity among various species of serum and IgA (+) indicates antibody reactivity to the corresponding target.)

WB (Western Blot) (Western Blot of Peroxidase conjugated Monkey IgM mu antibody. Lane 1: Monkey IgM. Lane 2: none. Load: 100 ng per lane. none. Peroxidase Goat secondary antibody at 1:1,000 for 60 min at RT. Block: Blocking buffer for 30 min at RT. Observed size: 72 kDa for Rabbit IgG..)

Compare (5 max)

Goat anti Monkey IgM (HRP), Secondary Antibody (Cat# AAA75533)

Full Name

Goat anti Monkey IgM (HRP)

Reactivity

Monkey IgM

Applications

Western Blot, Immunohistochemistry

Purity

Antibody purified by Ion Eschange Chromatography

Pricing

$925/1 mg $1,805/2x1 mg $2,630/3x1 mg $3,410/4x1 mg $3,930/5x1 mg

IF (Immunofluorescence) (Product: Rat Anti-Mouse k-PEAmount Used: 0.1 ug/10^6 cellsCells from BALB/c spleen were double-stained with Rat Anti-Mouse K-PE and Rat Anti-Mouse CD3e-FITC (Clone C363.29B) analyzed by flow cytometry.)

Compare (5 max)

Rat Anti-Mouse Kappa (kappa chain specific), Monoclonal Secondary Antibody (Cat# AAA78654)

Full Name

Rat Anti-Mouse Kappa-AP

Reactivity

Mouse

Applications

Immunofluorescence, Flow Cytometry

Pricing

$390/1 mL $755/2x1 mL $1,105/3x1 mL $1,430/4x1 mL $1,555/5x1 mL

Application Data (FLISA plate was coated with purified rat IgG AND IgM. Immunoglobulins were detected with serially diluted Goat Anti-Rat IgG-PE)

Compare (5 max)

Goat Anti-Rat IgG, Polyclonal Secondary Antibody (Cat# AAA78812)

Full Name

Goat Anti-Rat IgG-AP

Reactivity

Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunoblot

Purity

Affinity chromatography on pooled rat IgG covalently linked to agarose

Pricing

$280/1 mL $535/2x1 mL $780/3x1 mL $1,005/4x1 mL $1,030/5x1 mL

< >

<< Previous Next >>

Application Data

Application Data

Compare (5 max)

IgG (H&L), Secondary Antibody (Cat# AAA79057)

Full Name

Rabbit anti-Llama IgG (H&L) - Affinity Pure

Reactivity

Based on IEP, no reactivity is observed to: non-immunoglobulin Llama serum proteins.

Applications

Immuno-ElectronMicroscopy, Immunofluorescence, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

> 95% based on SDS-PAGE
Affinity purified using solid phase Llama IgG.

Pricing

$300/1 mg $575/2x1 mg $835/3x1 mg $1,085/4x1 mg $1,130/5x1 mg

SDS-PAGE

Compare (5 max)

Kappa (?) Light Chain, Secondary Antibody (Cat# AAA79071)

Full Name

Goat anti-Human Kappa (?) Light Chain - Affinity Pure, DyLight650 Conjugate, min x w/mouse serum

Reactivity

Human.
Cross Reactivity: Based on IEP, no reactivity is observed to:
non-immunoglobulin human serum immunoglobulins
heavy chains on all human immunoglobulins
lambda light chains on all human immunoglobulins mouse serum proteins

Applications

Immunoelectron Microscopy, Immunofluorescence, Flow Cytometry

Purity

Affinity purified antibody is > 95% based on SDS-PAGE
Affinity purified using solid phase Human Kappa Chain.

Pricing

$490/0.5 mg $955/2x0.5 mg $1,400/3x0.5 mg $1,815/4x0.5 mg $2,015/5x0.5 mg

< >

<< Previous Next >>

WB (Western Blot) (Goat Anti Rabbit IgG (HRP)secondary antibody was used at 1:40,000 to detect a rabbit primary antibody by Western Blot. p27 antibody (1:1000 RT 30 minutes) showed detection of 0.1 ug of recombinant p27 protein. Lane 1: Molecular weight markers. Lane 2: MBP-p27 fusion protein (arrow; expected MW: 73.3 kDa). Lane 3: MBP alone.)

DB (Dot Blot) (Shown here is a serial 1:1 dilution of Rabbit IgG protein (250ng starting total load) co-incubated with Goat anti Rabbit IgG (HRP) and Goat anti Rabbit (Dylight 649) 1:20,000. Blot was dried and imaged (A) on Biorad Versa Doc (30 sec, DyLight649), (B) LiCor Odyssey Reader (700 nm), (C) Rewetted incubated with Femtomax 110 reimaged using BioVersaDoc (for 60 sec), (D) Incubated with TMB substrate TMBM for 5 minutes and scanned, and (E) Rewetted for Chemiluminescence and imaged for 90 sec on the BioRad VersaDoc Imager.)

Compare (5 max)

Goat anti Rabbit IgG (H + L) (HRP), Secondary Antibody (Cat# AAA75487)

Full Name

Goat anti Rabbit IgG (H + L) (HRP)

Reactivity

Rabbit IgG (H + L)

Applications

Western Blot, Immunohistochemistry

Purity

Goat anti Rabbit IgG (H + L) (HRP) was purified by affinity chromatography.

Pricing

$965/1 mg $1,880/2x1 mg $2,745/3x1 mg $3,565/4x1 mg $4,115/5x1 mg

< >

<< Previous Next >>

DB (Dot Blot) (Dot Blot of Mouse IgG2a Antibody Alkaline Phosphatase Conjugated. Antigen: Mouse IgG2a Load: Lane 1 - 200ng Lane 2 - 66.7ng Lane 3 - 22.2ng Lane 4 - 7.4 Lane 2.5ng Primary antibody: none Secondary antibody: Mouse IgG2a Antibody Alkaline Phosphatase Conjugated at 1:1,000 for 60 min at RT. Block: MB-070 for 60 min at RT Reaction visualized using alkaline phosphatase substrate for 30 seconds at RT.)

WB (Western Blot) (Western Blot of Mouse IgG2a antibody Alkaline Phosphatase Conjugated. Lane 1: Mouse IgG2a. Lane 2: none. Load: 50ng per lane. none. Mouse IgG2a antibody Alkaline Phosphatase Conjugated at 1:1,000 o/n at 4 deg C. Block: Blocking buffer for 30 min at RT. Reaction visualized with alkaline phosphatase substrate for 30 seconds at RT. Size: 55kDa for Mouse IgG2a.)

Compare (5 max)

Rabbit anti Mouse IgG2a (Alk Phos), Secondary Antibody (Cat# AAA75495)

Full Name

Rabbit anti Mouse IgG2a (Alk Phos)

Reactivity

Murine IgG2a

Applications

Western Blot, Immunohistochemistry

Purity

Rabbit anti Mouse IgG2a (Alk Phos) was purified by affinity chromatography.

Pricing

$965/1 mg $1,880/2x1 mg $2,745/3x1 mg $3,565/4x1 mg $4,115/5x1 mg

< >

<< Previous Next >>

Application Data (Lamin B1 was detected in paraffin-embedded sections of human lung cancer tissues using rabbit anti- Lamin B1 Antigen Affinity purified polyclonal antibody at 1 ug/mL. DyLight 488 Conjugated Goat Anti-rabbit IgG secondary antibody was used to detect the primary antibody at 10 ug/mL.)

Application Data (GST3/GST was detected in paraffin-embedded sections of human intestinal cancer tissues using rabbit anti-GST3/GST Antigen Affinity purified polyclonal antibody at 1 ug/mL. DyLight-488 Conjugated Goat Anti-rabbit IgG secondary antibody was used to detect the primary antibody at 10ug/mL.)

Compare (5 max)

DyLight488 Conjugated Goat Anti-rabbit IgG, Polyclonal Secondary Antibody (Cat# AAA46012)

Full Name

DyLight488 Conjugated Goat Anti-rabbit IgG

Applications

Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry

Pricing

$275/0.5 mL $385/1 mL $745/2x1 mL $1,090/3x1 mL $1,410/4x1 mL $1,530/5x1 mL

< >

<< Previous Next >>

Application Data (MCM2 was detected in paraffin-embedded sections of human intestinal cancer tissues using rabbit anti- MCM2 Antigen Affinity purified polyclonal antibody at 1 ug/mL. TRITC Conjugated Goat Anti-rabbit IgG secondary antibody was used to detect the primary antibody at 30ug/mL.)

Application Data (Cpn10 was detected in paraffin-embedded sections of human mammary cancer tissues using rabbit anti- Cpn10 Antigen Affinity purified polyclonal antibody at 1 ug/mL. TRITC Conjugated Goat Anti-rabbit IgG secondary antibody was used to detect the primary antibody at 30 ug/mL.)

Compare (5 max)

TRITC Conjugated Goat Anti-rabbit IgG, Polyclonal Secondary Antibody (Cat# AAA45596)

Full Name

TRITC Conjugated Goat Anti-rabbit IgG secondary antibody

Applications

Immunocytochemistry, Immunohistochemistry, Immunohistochemistry

Pricing

$245/0.5 mL $385/1 mL $745/2x1 mL $1,090/3x1 mL $1,410/4x1 mL $1,530/5x1 mL

What Are Secondary Antibodies?

Secondary antibodies are immunoglobulins that bind to primary antibodies, which are directly bound to the target antigen. They are conjugated with either an enzyme, fluorophore, or biotin, and they are almost always only targeting the constant region (Fc) of the primary antibody. As a result, this amplifies the signal in various assays and helps researchers detect, quantify, and visualize specific antigens in a complex biological sample.

Key Applications of Secondary Antibodies

• Western blotting for protein detection and quantification.


• ELISA (Enzyme-Linked Immunosorbent Assay) for antigen-antibody interaction studies and quantification.


• Immunohistochemistry (IHC) and immunofluorescence imaging for tissue and cell analysis.


• Flow cytometry for cell surface and intracellular marker detection.


• Immunoprecipitation (IP) and chromatin immunoprecipitation (ChIP) assays.


• Signal amplification in various immunoassays.


• Multiplex assays using fluorophore-conjugated secondary antibodies.

Advantages of Using Secondary Antibodies

1. High sensitivity

   
   

   Multiple secondary antibodies can bind to a single primary antibody and amplify the signal. This is especially useful for detecting antigens even if they are in low concentrations.

   
   



2. Increased flexibility in assay design

   
   

   Secondary antibodies come pre-conjugated with labels, such as enzymes, fluorophores, biotin, and more. This provides researchers with flexibility to use in multiple applications. They also support multiplexing for the simultaneous detection of multiple targets.

   
   



3. Cost-effective

   
   

   Since the Fc domain remains constant within the same animal class, only one type of secondary antibody is needed to bind many types of primary antibodies. This reduces the cost of labeling multiple primary antibodies.

   
   



4. Better signal-to-noise ratio

   
   

   Secondary antibodies minimize the background noise. As a result, they provide clearer and more accurate results.

   
   



5. Versatility

   
   

   A single species- and isotype-specific secondary antibody can be used with any compatible primary antibody. This can help streamline reagent inventory and reduce cost and preparation time.

   
   



6. Easier detection

   
   

   Unlike primary antibodies, secondary antibodies’ main characteristic is that they will almost always be partnered with a component that can be used for detection. This can make detection and visualization easy.

   
   

Why Buy Secondary Antibodies from AAA Biotech?

• Highly Validated: Most of our secondary antibodies are thoroughly tested to ensure they work reliably across different experiments.


• Versatile Applications: Our secondary antibodies can be used in a wide range of research techniques, including (but not limited to) immunocytochemistry (ICC), ELISA, immunofluorescence (IF), immunohistochemistry (IHC), flow cytometry (FC), immunoprecipitation (IP), and Western blotting (WB).


• Support for Rare Species: We offer secondary antibodies specifically designed for rare or less common species—something many other suppliers don’t provide.


• Affordable Prices: Our secondary antibodies are available at competitive prices to support all research budgets.


• Quick and Convenient Ordering: Simple online ordering process with responsive customer support.


• Privacy: We respect your privacy and ensure your information is protected at every step of the ordering process, and in all other communication with our team members.

FAQ

1. Can I reuse a secondary antibody?

Not recommended for critical experiments, as reusing may compromise sensitivity and specificity.

2. What is the difference between primary and secondary antibodies?

Primary antibodies bind directly to the antigen. Secondary antibodies bind to the primary and are usually labeled for detection.

3. How long do secondary antibodies last?

When stored properly (usually at –20°C or 4°C, depending on formulation), they can last for years. Always refer to the product datasheet.

4. What happens if you use too much secondary antibody?

Excess can increase background noise, leading to a poor signal-to-noise ratio. Titrate to find the optimal concentration.

5. Can secondary antibodies be monoclonal?

Yes. While many are polyclonal, monoclonal secondary antibodies are available for consistent batch-to-batch performance.

6. How to find secondary antibodies?

Use our catalog filters to search by host species, conjugate type, target species, and application.

7. How to store secondary antibodies?

Store at recommended temperatures away from light. Avoid repeated freeze-thaw cycles.