Secondary Antibodies

At AAA Biotech, we provide a vast collection of high-quality, purified, conjugated and unconjugated secondary antibodies to use in various applications, such as Western Blotting, Flow Cytometry, Cell Imaging, or CUT & Tag.

Our secondary antibodies are available with a wide range of labels—such as HRP, AP, FITC, and biotin—to match your detection system and enhance signal sensitivity. Each antibody is rigorously tested to ensure high specificity and minimal cross-reactivity, delivering reliable performance across multiple species and assay types.

Whether you're conducting qualitative imaging or quantitative analysis, AAA Biotech has the right secondary antibody to support your research with consistency and precision. Our entire catalog of secondary antibodies is available for browsing through our main website.

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IF (Immunofluorescence) (Immunofluorescence using hCEC cells, 1st Ab (anti-beta-Actin at 1/250) and 2nd Ab (anti-mouse AB27488 at 1/1,000); cells were fixed with methanol;)

Reactivity Data

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IgG, Polyclonal Secondary Antibody (Cat# AAA63218)

Full Name

Anti-Mouse IgG, DyLight488

Reactivity

Mouse

Applications

Immunofluorescence, Western Blot

Purity

Epitope affinity purified

Pricing

$275/0.5 mg $525/2x0.5 mg $760/3x0.5 mg $985/4x0.5 mg $1,045/5x0.5 mg

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IF (Immunofluorescence) (Immunofluorescence - anti-mCherry Ab (AB9770) using hCEC cells transduced with mCherry-Rab5a; cells were fixed with methanol and mCherry Ab at 1/250; 2nd Ab goat anti-IgY (AB307488) at 1:1,000;)

IF (Immunofluorescence) (Immunofluorescence -Anti-mCherry Ab (AB9770) using hCEC cells transduced with mCherry-Rab5a; cells were fixed with methanol and mCherry Ab at 1/250; 2nd Ab goatAnti-IgY (AB307488) at 1:1,000;)

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IgY, Polyclonal Secondary Antibody (Cat# AAA63224)

Full Name

Anti-Chicken IgY, DyLight488

Reactivity

Chicken

Applications

Immunofluorescence, Western Blot

Purity

Epitope affinity purified

Pricing

$275/0.5 mg $525/2x0.5 mg $760/3x0.5 mg $985/4x0.5 mg $1,045/5x0.5 mg

Application Data

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Goat Anti-Mouse IgG1 (gamma 1 chain specific), Polyclonal Secondary Antibody (Cat# AAA78779)

Full Name

Goat Anti-Mouse IgG1, Human ads-APC

Reactivity

Mouse

Purity

Affinity chromatography on mouse IgG1 covalently linked to agarose

Pricing

$425/0.25 mg $830/2x0.25 mg $1,205/3x0.25 mg $1,565/4x0.25 mg $1,705/5x0.25 mg

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IF (Immunofluorescence) (Immunofluorescence in Drosophila larvaenmJ muscle 6/7 expressing mCherry in neurons (DyGlutmcherry) using 1st AbAnti-mCherry at 1/1,000 and 2nd AbAnti-goat IgY conjugated to DyLight488 at 1/500;)

Reactivity Data

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IgG, Polyclonal Secondary Antibody (Cat# AAA63210)

Full Name

Anti-Goat IgG, DyLight488

Reactivity

Goat

Applications

Immunofluorescence, Western Blot

Purity

Epitope affinity purified

Pricing

$275/0.5 mg $525/2x0.5 mg $760/3x0.5 mg $985/4x0.5 mg $1,045/5x0.5 mg

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IF (Immunofluorescence) (Immunofluorescence using hCEC cells, 1st Ab (anti-beta-Actin at 1/250) and 2nd Ab (anti-mouse AB27550 at 1/1,000); cells were fixed with methanol;)

IS (Immunostaining) (Immunostaining - C57BL/6J mouse retina labeled with: Green- 1st Ab Cone Arrestin (1/1,000); 2nd Ab goatAnti-rat (AB28488, 1/1,000), Red- 1st Ab Rhodopsin (1/1,000); 2nd Ab goatAnti-mouse (AB27550, 1/1,000), Blue- Nuclear staining (DAPI), scale bar = 40 um;)

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IgG, Polyclonal Secondary Antibody (Cat# AAA63219)

Full Name

Anti-Mouse IgG, DyLight550

Reactivity

Mouse

Applications

Immunofluorescence, Western Blot

Purity

Epitope affinity purified

Pricing

$275/0.5 mg $525/2x0.5 mg $760/3x0.5 mg $985/4x0.5 mg $1,045/5x0.5 mg

Application Data (FLISA plate was coated with purified rhesus IgG. Immunoglobulin was detected with Goat Anti-Rhesus IgG (H+L)-PE)

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Goat Anti-Rhesus IgG (H+L), Polyclonal Secondary Antibody (Cat# AAA78849)

Full Name

Goat Anti-Rhesus IgG (H+L)-HRP

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunoblot

Purity

Affinity chromatography on rhesus IgG covalently linked to agarose.

Pricing

$210/1 mL $410/2x1 mL $600/3x1 mL $760/5x1 mL $775/4x1 mL

IF (Immunofluorescence) (Figure 1: FITC staining on IHC-P,Sample: Rat Testis TissuePrimary Ab: 3ug/mL Rabbit Anti-Rat H3 Ab (Catalog: )Second Ab: 1:500 Dilution of FITC-Linked Guinea pig Anti-Rabbit IgG Ab (Catalog: AAA146597))

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IgG, Polyclonal Secondary Antibody (Cat# AAA146597)

Full Name

FITC-Linked Guinea pig Anti-Rabbit IgG Polyclonal Antibody

Reactivity

Rabbit

Applications

Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Antigen-specific Affinity Chromatography.

Pricing

$205/1 mL $390/5 mL $755/2x5 mL $1,105/3x5 mL $1,430/4x5 mL $1,725/5x5 mL

IF (Immunofluorescence) (Immunofluorescence Staining)

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Mouse Anti-Rat IgM (u chain specific), Monoclonal Secondary Antibody (Cat# AAA78695)

Full Name

Mouse Anti-Rat IgM-HRP

Reactivity

Rat

Applications

Immunofluorescence, Flow Cytometry

Pricing

$475/1 mL $925/2x1 mL $1,355/3x1 mL $1,755/4x1 mL $1,950/5x1 mL

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IHC (Immunohiostchemistry) ((Figure: DAB staining on IHC-P; Sample: Rat Brain Tissue.))

WB (Western Blot) (Figure 1: Used in Western Blot, Sample: Native Rabbit IgG ProteinAb: 1:2000 Dilution of HRP-Linked Guinea pig Anti-Rabbit Ab (Catalog: AAA145957))

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Guinea pig Anti-Rabbit IgG, Polyclonal Secondary Antibody (Cat# AAA145957)

Full Name

HRP-Linked Guinea pig Anti-Rabbit IgG Polyclonal Antibody

Reactivity

Rabbit

Applications

Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Antigen-specific Affinity Chromatography.

Pricing

$210/1 mL $415/5 mL $810/2x5 mL $1,175/3x5 mL $1,525/4x5 mL $1,840/5x5 mL

FLISA (FLISA plate was coated with purified rat IgG1, IgG2a, IgG2b, IgG2c, and IgM. Immunoglobulins were detected with serially diluted Mouse Anti-Rat IgG2c-FITC)

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Mouse Anti-Rat IgG2c (gamma 2c chain specific), Monoclonal Secondary Antibody (Cat# AAA78694)

Full Name

Mouse Anti-Rat IgG2c-UNLB

Reactivity

Rat

Applications

Flow Cytometry

Pricing

$340/0.5 mg $655/2x0.5 mg $955/3x0.5 mg $1,235/4x0.5 mg $1,315/5x0.5 mg

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IHC (Immunohistochemistry) ((Figure: FITC staining on IHC-P; Samples: Rat Lung Tissue.))

IHC (Immunohistochemisry) ((Figure: FITC staining on IHC-P; Samples: Rat Adrenal Gland Tissue.))

IHC (Immunohiostchemistry) ((Figure: FITC staining on IHC-P; Samples: Rat Spleen Tissue.))

IHC (Immunohistochemistry) ((Figure: FITC staining on IHC-P; Samples: Rat Kidney Tissue.))

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IgG, Polyclonal Secondary Antibody (Cat# AAA146595)

Full Name

FITC-Linked Rabbit Anti-Rat IgG Polyclonal Antibody

Reactivity

Rat

Applications

Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Antigen-specific affinity chromatography

Pricing

$205/1 mL $390/5 mL $755/2x5 mL $1,105/3x5 mL $1,430/4x5 mL $1,725/5x5 mL

WB (Western Blot) ((Figure. Used in Western Blot; Sample: Rabbit Serum.))

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Immunoglobulin G, Polyclonal Secondary Antibody (Cat# AAA147970)

Full Name

None-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

Reactivity

Rabbit

Applications

Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Antigen-specific Affinity Chromatography.

Pricing

$195/1 mL $365/5 mL $705/2x5 mL $1,030/3x5 mL $1,335/4x5 mL $1,600/5x5 mL

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WB (Western Blot) (Detection of Actin and hnRNP in HeLa Whole Cell Lysate. Primary Antibodies: cocktail of rabbit anti-Actin and mouse anti-hnRNP at 1 ug/ml each. Secondary Antibodies: cocktail of Dylight 488-conjugated goat anti-rabbit AAA210708 (AAA210708-5) (blue) and Dylight 680-conjugated goat anti-mouse (red) at 0.5 ug/ml each. Acquisition: Syngene G:Box, 6 seconds (blue) and 42 seconds (red).)

IF (Immunofluorescence) (Detection of human PCNA by immunofluorescence. Sample: FFPE section of human stomach carcinoma. Primary Antibody: Affinity purified rabbit anti-PCNA (Cat. No. Lot1) used at a dilution of 1:100. Secondary Antibody: Green-fluorescent goat anti-rabbit IgG-heavy and light chain, cross-adsorbed Antibody DyLight 488 Conjugated (AAA210708 Lot 6) used at a dilution of 1:100 (5ug/ml). Counterstain: DAPI (blue))

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IgG Heavy and Light Chain Cross-Adsorbed, Polyclonal Secondary Antibody (Cat# AAA210708)

Full Name

Goat anti-Rabbit IgG Heavy and Light Chain Cross-Adsorbed Antibody DyLight 488 Conjugated

Reactivity

Rabbit
Minimum Reactivity: Human, Mouse, Rat, Chicken, Bovine, Horse, Pig

Applications

Immunofluorescence, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purified

Pricing

$300/0.5 mg $575/2x0.5 mg $835/3x0.5 mg $1,085/4x0.5 mg $1,105/5x0.5 mg

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IF (Immunofluorescence) (Figure 3. IF analysis of NEFH using anti-NEFH antibody (MA1071).NEFH was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5ug/mL mouse anti-NEFH Antibody (MA1071) overnight at 4 degree C. DyLight 647 Conjugated Goat Anti-Mouse IgG (AAA127949) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IF (Immunofluorescence) (Figure 2. IF analysis of NEFH using anti-NEFH antibody (MA1071).NEFH was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5ug/mL mouse anti-NEFH Antibody (MA1071) overnight at 4 degree C. DyLight 647 Conjugated Goat Anti-Mouse IgG (AAA127949) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

WB (Western Blot) (Figure 1. Western blot analysis of Importin Beta/KPNB1 using anti-Importin Beta/KPNB1 antibody (M01851-2).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Caco-2 whole cell lysates,Lane 2: human K562 whole cell lysates,Lane 3: human A549 whole cell lysates,Lane 4: human 293T whole cell lysates,Lane 5: rat NRK whole cell lysates,Lane 6: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Importin Beta/KPNB1 antigen affinity purified monoclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Importin Beta/KPNB1 at approximately 97 kDa. The expected band size for Importin Beta/KPNB1 is at 97 kDa.)

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IgG, Polyclonal Secondary Antibody (Cat# AAA127949)

Full Name

DyLight 647 Conjugated AffiniPure Goat Anti-Mouse IgG (H+L)

Reactivity

Mouse

Applications

Western Blot, Immunofluorescence, Flow Cytometry

Pricing

$180/0.1 mL $350/2x0.1 mL $505/3x0.1 mL $620/5x0.1 mL $660/4x0.1 mL

Application Data

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Mouse Anti-Rat IgG2b (gamma 2b chain specific), Monoclonal Secondary Antibody (Cat# AAA78693)

Full Name

Mouse Anti-Rat IgG2b-FITC

Reactivity

Rat

Applications

Immunofluorescence, Flow Cytometry

Pricing

$390/0.5 mg $755/2x0.5 mg $1,105/3x0.5 mg $1,430/4x0.5 mg $1,555/5x0.5 mg

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WB (Western Blot) (Fluorescent western blot Analysis Demonstrating Diminished Cross-reactivity of donkey anti-rabbit IgG Cross-adsorbed Secondary Antibody with goat and mouse IgG. (A) HeLa whole cell lysate (30 ug) was blotted and incubated with a cocktail of mouse anti-hnRNP rabbit anti-actin and goat anti-GAPDH at 1 ug/ml each. For detection, the blot was incubated with 0.5 ug/ml donkey anti-rabbit IgG heavy and light chain cross-adsorbed antibody conjugated to Dylight 488 AAA210709. Donkey anti-rabbit IgG heavy and light chain cross-adsorbed antibody (AAA210709) specifically detected rabbit anti-actin (blue) and showed no cross-reactivity with the goat anti-GAPDH and mouse anti-hnRNP primary antibodies. (B) GAPDH, actin, and hnRNP were detected In a parallel strip incubated with the primary antibody cocktail used in (A), and a secondary antibody cocktail of Dylight 800-conjugated donkey anti-mouse (red), Dylight 488-conjugated donkey anti-rabbit AAA210709 (blue), and Dylight 488-conjugated donkey anti-goat (green) at 0.5 ug/ml each.)

IF (Immunofluorescence) (Detection of human PCNA by immunofluorescence. Samples: FFPE sections of human stomach carcinoma. Primary Antibody: Affinity purified rabbit anti-PCNA used at a dilution of 1:100. Secondary Antibody: Green-fluorescent donkey anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight 488 Conjugated (AAA210709) used at a dilution of 1:50 (10 ug/ml). Counterstain: DAPI (blue))

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IgG Heavy and Light Chain Cross-Adsorbed, Polyclonal Secondary Antibody (Cat# AAA210709)

Full Name

Donkey anti-Rabbit IgG Heavy and Light Chain Cross-Adsorbed Antibody DyLight 488 Conjugated

Reactivity

Rabbit
Minimum Reactivity: Human, Mouse, Rat, Chicken, Goat, Bovine, Pig

Applications

Immunofluorescence, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purified

Pricing

$300/0.5 mg $575/2x0.5 mg $835/3x0.5 mg $1,085/4x0.5 mg $1,105/5x0.5 mg

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WB (Western Blot) (Detection of human NF-H using mouse anti-Chicken IgY secondary antibody by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, OVCAR-8, HEK293T, SK-N-BE(2), and HeLa cells prepared using NETN lysis buffer. Primary: Chicken anti-NF-H antibody. Secondary: mouse anti-Chicken IgY Light Chain monoclonal antibody [1Y-263] (AAA213527 lot 1) used at 1:1000. Tertiary: HRP-conjugated goat anti-mouse IgG . Detection: Chemiluminescence with an exposure time of 1 second.)

IHC (Immunohiostchemistry) (Detection of human NF-H using mouse anti-chicken IgY secondary antibody by immunohistochemistry. Sample: FFPE section of human cerebellum. Primary: Chicken anti-NF-H antibody. Secondary: Mouse anti-Chicken IgY Light Chain monoclonal antibody [1Y-263)] (AAA213527 lot 1). Tertiary: HRP-conjugated goat anti-mouse IgG .)

ICC (Immunocytochemistry) (Detection of human NF-H using mouse anti-chicken IgY secondary antibody by immunocytochemistry. Sample: FFPE section of SK-N-BE(2) cells. Primary: Chicken anti-NF-H antibody. Secondary: Mouse anti-Chicken IgY Light Chain monoclonal antibody [1Y-263)] (AAA213527 lot 1). Tertiary: HRP-conjugated goat anti-mouse IgG .)

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IgY Light Chain, Monoclonal Secondary Antibody (Cat# AAA213527)

Full Name

Mouse anti-Chicken IgY Light Chain Monoclonal Antibody [1Y-263]

Reactivity

Chicken

Applications

Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Purified

Pricing

$265/0.01 mL $655/0.1 mL $955/5x0.01 mL $1,270/2x0.1 mL $1,860/3x0.1 mL $2,410/4x0.1 mL $2,730/5x0.1 mL

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WB (Western Blot) (Detection of hnRNP, Actin, and GAPDH in HeLa Whole Cell Lysate. Primary Antibodies: cocktail of mouse anti-hnRNP , rabbit anti-actin , and goat anti-GAPDH at 1 ug/ml each. Secondary Antibodies: cocktail of Dylight 488-conjugated donkey anti-mouse AAA213716 (AAA213716-5) (blue), Dylight 680-conjugated donkey anti-rabbit (green), and Dylight 800-conjugated donkey anti-goat A50-201D8 (A50-201D8-1) (red) at 0.5 ug/ml each. Acquisition: Syngene G:Box, 34 seconds (blue), 20 seconds (green), and 50 seconds (red).)

IF (Immunofluorescence) (Detection of human p53 by immunofluorescence. Sample: FFPE section of human ovarian carcinoma. Primary Antibody: mouse anti-p53 (clone DO-1) used at a dilution of 1:100. Secondary Antibody: Green-fluorescent donkey anti-mouse IgG-heavy and light chain cross-adsorbed Antibody DyLight 488 Conjugated (AAA213716) used at a dilution of 1:50 (10 ug/ml). Counterstain: DAPI (blue))

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IgG Heavy and Light Chain Cross-Adsorbed, Polyclonal Secondary Antibody (Cat# AAA213716)

Full Name

Donkey anti-Mouse IgG Heavy and Light Chain Cross-Adsorbed Antibody DyLight 488 Conjugated

Reactivity

Mouse
Minimum Reactivity: Human, Rat, Chicken, Sheep, Goat, Bovine, Rabbit

Applications

Immunofluorescence, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purified

Pricing

$300/0.5 mg $575/2x0.5 mg $835/3x0.5 mg $1,085/4x0.5 mg $1,105/5x0.5 mg

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WB (Western Blot) (Detection of GAPDH and hnRNP in HeLa Whole Cell Lysate. Primary Antibodies: cocktail of goat anti-GAPDH and mouse anti-hnRNP at 1 ug/ml each. Secondary Antibodies: cocktail of Dylight 488-conjugated rabbit anti-goat AAA213528 (AAA213528-2) (blue) and Dylight 680-conjugated rabbit anti-mouse (red) at 0.5 ug/ml each. Acquisition: Syngene G:Box, 52 seconds (blue) and 77 seconds (red).)

FCM/FACS (Flow Cytometry) (Detection of human CD137 (shaded) in HDLM-2 cells by flow cytometry. Antibody: Goat anti-CD137 antibody or isotype control (unshaded). Secondary: DyLight 488-conjugated rabbit anti-goat IgG (AAA213528).)

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IgG Heavy and Light Chain, Polyclonal Secondary Antibody (Cat# AAA213528)

Full Name

Rabbit anti-Goat IgG Heavy and Light Chain Antibody DyLight 488 Conjugated

Reactivity

Goat

Applications

Immunofluorescence, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purified,

Pricing

$280/0.5 mg $535/2x0.5 mg $780/3x0.5 mg $1,005/4x0.5 mg $1,015/5x0.5 mg

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WB (Western Blot) (Detection of human PCNA by western blot with HRP-conjugated Goat anti-Mouse IgG Heavy and Light Chain Antibody. Samples: Whole cell lysate (50 ug) from Hela, Jurkat, K-562, A549, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Mouse anti-PCNA Monoclonal Antibody [PC10] used for WB at 1:1000. Secondary: HRP-conjugated Goat anti-Mouse IgG Heavy and Light Chain Antibody (AAA213712). Detection: Chemiluminescence with an exposure time of 3 seconds.)

IHC (Immunohistochemistry) (Detection of human p53 by immunoperoxidase. Samples: FFPE serial sections of human stomach carcinoma. Primary Antibody: mouse anti-p53 (Clone DO-1) used at a dilution of 1:100. Secondary Antibody: goat anti-mouse IgG-heavy and light chain Antibody HRP Conjugated (AAA213712) used at a dilution of 1:200 (5ug/ml). Detection: DAB)

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IgG Heavy and Light Chain, Polyclonal Secondary Antibody (Cat# AAA213712)

Full Name

Goat anti-Mouse IgG Heavy and Light Chain Antibody HRP Conjugated

Reactivity

Mouse

Applications

Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purified

Pricing

$250/1 mg $490/2x1 mg $715/3x1 mg $925/5x1 mg $930/4x1 mg

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WB (Western Blot) (Detection of human PCNA by western blot with HRP-conjugated Rabbit anti-Mouse IgG Heavy and Light Chain Antibody. Samples: Whole cell lysate (50 ug) from Hela, Jurkat, K-562, A549, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Mouse anti-PCNA Monoclonal Antibody [PC10] used for WB at 1:1000. Secondary: HRP-conjugated Rabbit anti-Mouse IgG Heavy and Light Chain Antibody (AAA213713). Detection: Chemiluminescence with an exposure time of 3 seconds.)

IHC (Immunohistochemistry) (Detection of human p53 by immunoperoxidase. Sample: FFPE section of human stomach carcinoma. Primary Antibody: mouse anti-p53 (Clone DO-1) used at a dilution of 1:100. Secondary Antibody: Rabbit anti-mouse IgG-heavy and light chain Antibody HRP Conjugated (AAA213713) used at a dilution of 1:100 (10ug/ml). Detection: DAB Counterstain: IHC hematoxylin (blue))

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IgG Heavy and Light Chain, Polyclonal Secondary Antibody (Cat# AAA213713)

Full Name

Rabbit anti-Mouse IgG Heavy and Light Chain Antibody HRP Conjugated

Reactivity

Mouse

Applications

Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purified,

Pricing

$250/1 mg $490/2x1 mg $715/3x1 mg $925/5x1 mg $930/4x1 mg

What Are Secondary Antibodies?

Secondary antibodies are immunoglobulins that bind to primary antibodies, which are directly bound to the target antigen. They are conjugated with either an enzyme, fluorophore, or biotin, and they are almost always only targeting the constant region (Fc) of the primary antibody. As a result, this amplifies the signal in various assays and helps researchers detect, quantify, and visualize specific antigens in a complex biological sample.

Key Applications of Secondary Antibodies

• Western blotting for protein detection and quantification.


• ELISA (Enzyme-Linked Immunosorbent Assay) for antigen-antibody interaction studies and quantification.


• Immunohistochemistry (IHC) and immunofluorescence imaging for tissue and cell analysis.


• Flow cytometry for cell surface and intracellular marker detection.


• Immunoprecipitation (IP) and chromatin immunoprecipitation (ChIP) assays.


• Signal amplification in various immunoassays.


• Multiplex assays using fluorophore-conjugated secondary antibodies.

Advantages of Using Secondary Antibodies

1. High sensitivity

   
   

   Multiple secondary antibodies can bind to a single primary antibody and amplify the signal. This is especially useful for detecting antigens even if they are in low concentrations.

   
   



2. Increased flexibility in assay design

   
   

   Secondary antibodies come pre-conjugated with labels, such as enzymes, fluorophores, biotin, and more. This provides researchers with flexibility to use in multiple applications. They also support multiplexing for the simultaneous detection of multiple targets.

   
   



3. Cost-effective

   
   

   Since the Fc domain remains constant within the same animal class, only one type of secondary antibody is needed to bind many types of primary antibodies. This reduces the cost of labeling multiple primary antibodies.

   
   



4. Better signal-to-noise ratio

   
   

   Secondary antibodies minimize the background noise. As a result, they provide clearer and more accurate results.

   
   



5. Versatility

   
   

   A single species- and isotype-specific secondary antibody can be used with any compatible primary antibody. This can help streamline reagent inventory and reduce cost and preparation time.

   
   



6. Easier detection

   
   

   Unlike primary antibodies, secondary antibodies’ main characteristic is that they will almost always be partnered with a component that can be used for detection. This can make detection and visualization easy.

   
   

Why Buy Secondary Antibodies from AAA Biotech?

• Highly Validated: Most of our secondary antibodies are thoroughly tested to ensure they work reliably across different experiments.


• Versatile Applications: Our secondary antibodies can be used in a wide range of research techniques, including (but not limited to) immunocytochemistry (ICC), ELISA, immunofluorescence (IF), immunohistochemistry (IHC), flow cytometry (FC), immunoprecipitation (IP), and Western blotting (WB).


• Support for Rare Species: We offer secondary antibodies specifically designed for rare or less common species—something many other suppliers don’t provide.


• Affordable Prices: Our secondary antibodies are available at competitive prices to support all research budgets.


• Quick and Convenient Ordering: Simple online ordering process with responsive customer support.


• Privacy: We respect your privacy and ensure your information is protected at every step of the ordering process, and in all other communication with our team members.

FAQ

1. Can I reuse a secondary antibody?

Not recommended for critical experiments, as reusing may compromise sensitivity and specificity.

2. What is the difference between primary and secondary antibodies?

Primary antibodies bind directly to the antigen. Secondary antibodies bind to the primary and are usually labeled for detection.

3. How long do secondary antibodies last?

When stored properly (usually at –20°C or 4°C, depending on formulation), they can last for years. Always refer to the product datasheet.

4. What happens if you use too much secondary antibody?

Excess can increase background noise, leading to a poor signal-to-noise ratio. Titrate to find the optimal concentration.

5. Can secondary antibodies be monoclonal?

Yes. While many are polyclonal, monoclonal secondary antibodies are available for consistent batch-to-batch performance.

6. How to find secondary antibodies?

Use our catalog filters to search by host species, conjugate type, target species, and application.

7. How to store secondary antibodies?

Store at recommended temperatures away from light. Avoid repeated freeze-thaw cycles.