anti-human IgG Secondary Antibody
Mouse secondary antibody to human IgG (Fc)-Unconjugated
The optimal dilutions should be determined by end user.
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Product Notes
The anti-human IgG (Catalog #AAA13530) is a Secondary Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse secondary antibody to human IgG (Fc)-Unconjugated reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's anti-human IgG can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Other applications have not been tested. The optimal dilutions should be determined by end user. . Researchers should empirically determine the suitability of the anti-human IgG for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "anti-human IgG, Monoclonal Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Frequently Asked Questions
What is the anti-IgG antibody?
Anti-IgG antibodies are secondary antibodies that recognize and bind to the immunoglobulin G (IgG) class of primary antibodies. They bind to both heavy and light chains of IgG for indirect immunodetection applications.
What is an HRP secondary antibody?
HRP (horseradish peroxidase) secondary antibody is an enzyme-conjugated antibody where HRP, a 44 kDa glycoprotein enzyme, is chemically linked to the secondary antibody. HRP catalyzes reactions with chromogenic substrates (TMB, DAB, ABTS) to produce visible signals in ELISA, Western blots, and IHC.
What applications is this anti-human IgG secondary antibody suitable for?
Anti-human IgG secondary antibodies are suitable for Western blotting, ELISA, immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF), flow cytometry, and protein array applications.
What conjugate formats are available (HRP, FITC, Alexa Fluor, etc.)?
Available formats include HRP (horseradish peroxidase), AP (alkaline phosphatase), FITC (fluorescein isothiocyanate), and Alexa Fluor dyes (488, 555, 594, 647, etc.). Each conjugate format optimizes signal detection for specific applications.
Is the antibody cross-adsorbed to reduce species cross-reactivity?
Yes, cross-adsorbed anti-human IgG secondary antibodies are treated to remove reactivity against other species like bovine, chicken, horse, mouse, rabbit, and rat. Cross-adsorption ensures species-specific detection and reduces background in multiplex experiments.
How should the secondary antibody be stored for long-term stability?
Store at 4°C short-term (days to weeks) with preservatives like sodium azide. For long-term storage, freeze at –20°C or –80°C, depending on formulation. Avoid repeated freeze-thaw cycles. Lyophilized formats maintain stability for 3–5 years at –20°C.
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