Polyclonal Antibodies

At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.

Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Figure 10. IHC analysis of COX IV using anti-COX IV antibody (AAA19173).COX IV was detected in paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-COX IV Antibody (AAA19173) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 10. IHC analysis of COX IV using anti-COX IV antibody (AAA19173).COX IV was detected in paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-COX IV Antibody (AAA19173) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistchemistry) (Figure 9. IHC analysis of COX IV using anti-COX IV antibody (AAA19173).COX IV was detected in paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-COX IV Antibody (AAA19173) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 8. IHC analysis of COX IV using anti-COX IV antibody (AAA19173).COX IV was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-COX IV Antibody (AAA19173) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of COX IV using anti-COX IV antibody (AAA19173).COX IV was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-COX IV Antibody (AAA19173) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of COX IV using anti-COX IV antibody (AAA19173).COX IV was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-COX IV Antibody (AAA19173) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of COX IV using anti-COX IV antibody (AAA19173).COX IV was detected in paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-COX IV Antibody (AAA19173) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of COX IV using anti-COX IV antibody (AAA19173).COX IV was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-COX IV Antibody (AAA19173) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of COX IV using anti-COX IV antibody (AAA19173).COX IV was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-COX IV Antibody (AAA19173) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

WB (Western Blot) (Figure 2. Western blot analysis of COX IV using anti-COX IV antibody (AAA19173).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat heart tissue lysates,Lane 2: mouse heart tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COX IV antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for COX IV at approximately 17KD. The expected band size for COX IV is at 20KD.)

WB (Western Blot) (Figure 1. Western blot analysis of COX IV using anti-COX IV antibody (AAA19173).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HepG2 whole cell lysates,Lane 2: human SGC-7901 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COX IV antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for COX IV at approximately 17KD. The expected band size for COX IV is at 20KD.)

Compare (5 max)

COX IV, Polyclonal Antibody (Cat# AAA19173)

Full Name

Anti-COX IV Picoband Antibody

Gene Names

COX4I1; COX4; COXIV; COX4-1; COXIV-1; COX IV-1

Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins.

Applications

EIA, IHC, WB

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

< >

<< Previous Next >>

WB (Western Blot) (WB Suggested Anti-FER1L3 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: MCF7 cell lysate.MYOF is strongly supported by BioGPS gene expression data to be expressed in MCF7)

WB (Western Blot) (Host: RabbitTarget Name: MYOFSample Type: Human Fetal MuscleAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: MYOFSample Type: Human Fetal LiverAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: MYOFSample Type: Human Fetal HeartAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: MYOFSample Type: Human Fetal BrainAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: MYOFSample Type: HelaAntibody Dilution: 1.0ug/mlMYOF is strongly supported by BioGPS gene expression data to be expressed in HeLa)

WB (Western Blot) (Host: RabbitTarget Name: MYOFSample Type: 721_BAntibody Dilution: 1.0ug/mlMYOF is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells)

WB (Western Blot) (Host: RabbitTarget Name: CHADSample Type: Human Adult PlacentaAntibody Dilution: 1.0ug/ml)

Compare (5 max)

FER1L3, Polyclonal Antibody (Cat# AAA23526)

Full Name

FER1L3 antibody - C-terminal region

Gene Names

MYOF; FER1L3

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish

Applications

WB

Purity

Affinity Purified

Pricing

$530/0.1 mL $1,005/2x0.1 mL $1,455/3x0.1 mL $1,885/4x0.1 mL $2,140/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

Compare (5 max)

TALL-1, Polyclonal Antibody (Cat# AAA29164)

Full Name

TALL-1 Polyclonal Antibody

Gene Names

TNFSF13B; DTL; BAFF; BLYS; CD257; TALL1; THANK; ZTNF4; TALL-1; TNFSF20

Reactivity

Human, Mouse

Applications

WB, IHC-P

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of mouse breast cells using PMAIP1 at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of mouse breast cells using PMAIP1 at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of mouse breast cells using PMAIP1 at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of rat breast cells using PMAIP1 at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of Jurkat cells using PMAIP1 at dilution of 1:100. Blue: DAPI for nuclear staining.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using PMAIP1 at 1:1000 dilution.)

Compare (5 max)

PMAIP1, Polyclonal Antibody (Cat# AAA30715)

Full Name

PMAIP1 Rabbit Polyclonal Antibody

Gene Names

PMAIP1; APR; NOXA

Reactivity

Human, Mouse, Rat

Applications

WB, IF

Purity

Affinity purification

Pricing

$360/0.05 mL $450/0.1 mL $855/2x0.1 mL $1,230/3x0.1 mL $1,595/4x0.1 mL $1,750/5x0.1 mL

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of A549 cells using anti-DR6/TNFRSF21 antibody (AAA19292).Overlay histogram showing A549 cells stained with AAA19292 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DR6/TNFRSF21 Antibody (AAA19292, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of DR6/TNFRSF21 using anti-DR6/TNFRSF21 antibody (AAA19292).DR6/TNFRSF21 was detected in paraffin-embedded section of human melanoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-DR6/TNFRSF21 Antibody (AAA19292) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of DR6/TNFRSF21 using anti-DR6/TNFRSF21 antibody (AAA19292).DR6/TNFRSF21 was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-DR6/TNFRSF21 Antibody (AAA19292) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of DR6/TNFRSF21 using anti-DR6/TNFRSF21 antibody (AAA19292).DR6/TNFRSF21 was detected in paraffin-embedded section of human renal carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-DR6/TNFRSF21 Antibody (AAA19292) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of DR6/TNFRSF21 using anti-DR6/TNFRSF21 antibody (AAA19292).DR6/TNFRSF21 was detected in paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-DR6/TNFRSF21 Antibody (AAA19292) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of DR6/TNFRSF21 using anti-DR6/TNFRSF21 antibody (AAA19292).DR6/TNFRSF21 was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-DR6/TNFRSF21 Antibody (AAA19292) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of DR6/TNFRSF21 using anti-DR6/TNFRSF21 antibody (AAA19292).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human K562 whole cell lysatesLane 2: human Raji whole cell lysatesLane 3: human Sw620 whole cell lysatesLane 4: rat brain tissue lysatesLane 5: rat C6 whole cell lysatesLane 6: mouse brain tissue lysatesLane 7: mouse Raw164. 7 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-DR6/TNFRSF21antigen affinity purified polyclonal antibody (Catalog # AAA19292) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for DR6/TNFRSF21 at approximately 80-120KD. The expected band size for DR6/TNFRSF21 is at 72KD.)

Compare (5 max)

DR6/TNFRSF21, Polyclonal Antibody (Cat# AAA19292)

Full Name

Anti-DR6/TNFRSF21 Antibody

Gene Names

TNFRSF21; DR6; CD358; BM-018

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, FC/FACS/FCM

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of HEL cells using anti-Annexin-4/ANXA4 antibody (AAA19529).Overlay histogram showing HEL cells stained with AAA19529 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Annexin-4/ANXA4 Antibody (AAA19529, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of Annexin-4/ANXA4 using anti-Annexin-4/ANXA4 antibody (AAA19529).Annexin-4/ANXA4 was detected in a paraffin-embedded section of human thyroiditis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-Annexin-4/ANXA4 Antibody (AAA19529) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of Annexin-4/ANXA4 using anti-Annexin-4/ANXA4 antibody (AAA19529).Annexin-4/ANXA4 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-Annexin-4/ANXA4 Antibody (AAA19529) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of Annexin-4/ANXA4 using anti-Annexin-4/ANXA4 antibody (AAA19529).Annexin-4/ANXA4 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-Annexin-4/ANXA4 Antibody (AAA19529) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of Annexin-4/ANXA4 using anti-Annexin-4/ANXA4 antibody (AAA19529).Annexin-4/ANXA4 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-Annexin-4/ANXA4 Antibody (AAA19529) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of Annexin-4/ANXA4 using anti-Annexin-4/ANXA4 antibody (AAA19529).Annexin-4/ANXA4 was detected in a paraffin-embedded section of human leiomyoma of the vulva tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-Annexin-4/ANXA4 Antibody (AAA19529) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of Annexin-4/ANXA4 using anti-Annexin-4/ANXA4 antibody (AAA19529).Annexin-4/ANXA4 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-Annexin-4/ANXA4 Antibody (AAA19529) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of Annexin-4/ANXA4 using anti-Annexin-4/ANXA4 antibody (AAA19529).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human HepG2 whole cell lysates,Lane 2: human A549 whole cell lysates,Lane 3: human THP-1 whole cell lysates,Lane 4: human Hacat whole cell lysates,Lane 5: rat stomach tissue lysates,Lane 6: mouse lung tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Annexin-4/ANXA4 antigen affinity purified polyclonal antibody (#AAA19529) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Annexin-4/ANXA4 at approximately 36 kDa. The expected band size for Annexin-4/ANXA4 is at 36 kDa.)

Compare (5 max)

Annexin-4/ANXA4, Polyclonal Antibody (Cat# AAA19529)

Full Name

Anti-Annexin-4/ANXA4 Antibody Picoband

Gene Names

ANXA4; ANX4; PIG28; ZAP36; HEL-S-274

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of RH35 cells using anti-C1orf77/FOP/CHTOP antibody (AAA19587).Overlay histogram showing RH35 cells stained with AAA19587 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-C1orf77/FOP/CHTOP Antibody (AAA19587, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of THP-1 cells using anti-C1orf77/FOP/CHTOP antibody (AAA19587).Overlay histogram showing THP-1 cells stained with AAA19587 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-C1orf77/FOP/CHTOP Antibody (AAA19587, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of C1orf77/FOP/CHTOP using anti-C1orf77/FOP/CHTOP antibody (AAA19587).C1orf77/FOP/CHTOP was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-C1orf77/FOP/CHTOP Antibody (AAA19587) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of C1orf77/FOP/CHTOP using anti-C1orf77/FOP/CHTOP antibody (AAA19587).C1orf77/FOP/CHTOP was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-C1orf77/FOP/CHTOP Antibody (AAA19587) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of C1orf77/FOP/CHTOP using anti-C1orf77/FOP/CHTOP antibody (AAA19587).C1orf77/FOP/CHTOP was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-C1orf77/FOP/CHTOP Antibody (AAA19587) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of C1orf77/FOP/CHTOP using anti-C1orf77/FOP/CHTOP antibody (AAA19587).C1orf77/FOP/CHTOP was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-C1orf77/FOP/CHTOP Antibody (AAA19587) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of C1orf77/FOP/CHTOP using anti-C1orf77/FOP/CHTOP antibody (AAA19587).C1orf77/FOP/CHTOP was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-C1orf77/FOP/CHTOP Antibody (AAA19587) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (AAA19587-CHTOP-primary-antibodies-IHC-testing-7.jpg)

WB (Western Blot) (Figure 1. Western blot analysis of C1orf77/FOP/CHTOP using anti-C1orf77/FOP/CHTOP antibody (AAA19587).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: monkey COS-7 whole cell lysates,Lane 3: human THP-1 whole cell lysates,Lane 4: human MOLT-4 whole cell lysates,Lane 5: human RT4 whole cell lysates,Lane 6: human HL-60 whole cell lysates,Lane 7: human MCF-7 whole cell lysates,Lane 8: rat brain tissue lysates,Lane 9: rat PC-12 whole cell lysates,Lane 10: mouse spleen tissue lysates,Lane 11: mouse brain tissue lysates,Lane 12: mouse lung tissue lysates,Lane 13: mouse L929 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C1orf77/FOP/CHTOP antigen affinity purified polyclonal antibody (#AAA19587) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for C1orf77/FOP/CHTOP at approximately 28 kDa. The expected band size for C1orf77/FOP/CHTOP is at 26 kDa.)

Compare (5 max)

C1orf77/FOP/CHTOP, Polyclonal Antibody (Cat# AAA19587)

Full Name

Anti-C1orf77/FOP/CHTOP Antibody Picoband

Gene Names

CHTOP; FOP; SRAG; SRAG-3; SRAG-5; pp7704; C1orf77; FL-SRAG

Reactivity

Human, Monkey, Mouse, Rat

Applications

WB, IHC, FC/FACS

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

< >

<< Previous Next >>

WB (Western Blot) (WB Suggested Anti-FOXA1 antibody Titration: 1 ug/mLSample Type: Human liver)

WB (Western Blot) (WB Suggested Anti-FOXA1 antibody Titration: 1 ug/mLSample Type: Human heart)

WB (Western Blot) (WB Suggested Anti-FOXA1 Antibody Titration: 0.2-1 ug/mlPositive Control: Transfected 293T)

WB (Western Blot) (WB Suggested Anti-FOXA1 AntibodyPositive Control: Lane 1: 20ug MCF7 cell lysates Lane 2: 20ug MCF7 cell lysates Lane 3: 20ug MCF7 cell lysates Lane 4: 20ug MCF7 cell lysates Lane 5: 20ug MCF7 with FoxA1 knockdown Lane 6: 20ug MCF7 with FoxA1 knockdownPrimary Antibody Dilution : 1:1000Secondary Antibody : Anti-rabbit-HRPSecondry Antibody Dilution : 1:2000Submitted by: Fu, Xiaoyong, Baylor College of MedicineFOXA1 is supported by BioGPS gene expression data to be expressed in MCF7)

WB (Western Blot) (Host: MouseTarget Name: FOXA1Sample Tissue: Mouse LiverAntibody Dilution: 1ug/ml)

IHC (Immunohistochemistry) (Rabbit Anti-FOXA1 antibodyFormalin Fixed Paraffin Embedded Tissue: Human Adult heartObserved Staining: Nuclei in adipocytes but not in cardiomyocytesPrimary Antibody Concentration: 1:100Secondary Antibody: Donkey anti-Rabbit-Cy2/3Secondary Antibody Concentration: 1:200Magnification: 20XExposure Time: 0.5 - 2.0 secProtocol located in Reviews and Data.)

Compare (5 max)

FOXA1, Polyclonal Antibody (Cat# AAA23415)

Full Name

FOXA1 antibody - C-terminal region

Gene Names

FOXA1; HNF3A; TCF3A

Reactivity

Cow, Dog, Guinea Pig, Human, Mouse, Rat, Yeast, Zebrafish

Applications

WB, IHC

Purity

Affinity Purified

Pricing

$530/0.1 mL $1,005/2x0.1 mL $1,455/3x0.1 mL $1,885/4x0.1 mL $2,140/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human lung using TXN Polyclonal Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded Rat heart using TXN Polyclonal Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Rat brain using TXN Polyclonal Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Rat testis using TXN Polyclonal Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Rat liver using TXN Polyclonal Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Rat lung using TXN Polyclonal Antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines using TXN Polyclonal Antibody at dilution of 1:1000.)

Compare (5 max)

TXN, Polyclonal Antibody (Cat# AAA22183)

Full Name

TXN Polyclonal Antibody

Gene Names

TXN; TRX; TRDX; TRX1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC

Purity

Affinity purification

Pricing

$330/0.06 mL $445/0.12 mL $675/0.2 mL $1,290/2x0.2 mL $1,875/3x0.2 mL $2,420/4x0.2 mL $2,905/5x0.2 mL

< >

<< Previous Next >>

WB (Western Blot) (Figure 7. Western blot analysis of PLS1 using anti-PLS1 antibody (AAA19893).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human A431 whole cell lysates,Lane 2: human RT4 whole cell lysates,Lane 3: human A549 whole cell lysates,Lane 4: human Caco-2 whole cell lysates,Lane 5: mouse small intestine tissue lysates,Lane 6: rat stomach tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLS1 antigen affinity purified polyclonal antibody (AAA19893) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-DyLight 647 Conjugated secondary antibody at a dilution of 1:2000 for 1.5 hour at RT. A specific band was detected for PLS1 at approximately 70 kDa. The expected band size for PLS1 is at 70 kDa.)

FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of RT4 cells using anti-PLS1 antibody (AAA19893).Overlay histogram showing RT4 cells stained with AAA19893 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PLS1 Antibody (AAA19893, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 5. IF analysis of PLS1 using anti-PLS1 antibody (AAA19893).PLS1 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PLS1 Antibody (AAA19893) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PLS1 Antibody (AAA19893) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PLS1 Antibody (AAA19893) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human RT4 whole cell lysates,Lane 3: human A549 whole cell lysates,Lane 4: human Caco-2 whole cell lysates,Lane 5: rat small intestine tissue lysates,Lane 6: rat stomach tissue lysates,Lane 7: mouse small intestine tissue lysates,Lane 8: mouse stomach tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLS1 antigen affinity purified polyclonal antibody (#AAA19893) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PLS1 at approximately 70 kDa. The expected band size for PLS1 is at 70 kDa.)

Compare (5 max)

PLS1, Polyclonal Antibody (Cat# AAA19893)

Full Name

Anti-PLS1 Antibody Picoband

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ICC, IF, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

< >

<< Previous Next >>

WB (Western Blot) (Detection of mouse RPA32 by western blot. Samples: Whole cell lysate (10 ug) from CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPA32 antibody (AAA23770 lot 4) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)

WB (Western Blot) (Detection of human RPA32 by western blot. Samples: Whole cell lysate (10 ug) from HEK293T, HeLa, Jurkat, and U2OS cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPA32 antibody (AAA23770 lot 4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)

IP (Immunoprecipitation) (Detection of human RPA32 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells treated with 100 uM etoposide for 4 hours (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-RPA32 antibody (AAA23770 lot 4) used for IP at 6 ug per reaction. RPA32 was also immunoprecipitated by rabbit anti-Phospho RPA32 (S4/S8) antibody . For blotting immunoprecipitated RPA32, AAA23770 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)

IHC (Immunohistochemistry) (Detection of mouse RPA32 by immunohistochemistry. Sample: FFPE section of mouse kidney. Antibody: Affinity purified rabbit anti-RPA32 (AAA23770 Lot 4) used at a dilution of 1:5,000 (0.2 ug/ml). Detection: DAB)

IHC (Immunohistochemistry) (Detection of human RPA32 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-RPA32 (AAA23770 Lot 4) used at a dilution of 1:5,000 (0.2 ug/ml). Detection: DAB)

FCM (Flow Cytometry) (Detection of human RPA32 (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-RPA32 polyclonal antibody (AAA23770) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)

Compare (5 max)

RPA32, Polyclonal Antibody (Cat# AAA23770)

Full Name

Rabbit anti-RPA32 Antibody, Affinity Purified

Gene Names

RPA2; REPA2; RPA32

Reactivity

Human, Mouse

Applications

WB, IP, IHC, FC/FACS

Purity

Antigen Affinity Purified

Pricing

$290/0.01 mg $630/0.1 mg $965/5x0.01 mg $1,200/2x0.1 mg $1,735/3x0.1 mg $2,245/4x0.1 mg $2,500/5x0.1 mg

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of L929 cells using CSK Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse spleen using [KO Validated] CSK Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon carcinoma using [KO Validated] CSK Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat spleen using [KO Validated] CSK Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines using CSK Polyclonal Antibody at 1:1000 dilution.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines using CSK Polyclonal Antibody at 1:1000 dilution.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines using CSK Polyclonal Antibody at 1:1000 dilution.)

Compare (5 max)

CSK, Polyclonal Antibody (Cat# AAA22262)

Full Name

CSK Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

Purity

Affinity purification

Pricing

$325/0.06 mL $435/0.12 mL $655/0.2 mL $1,245/2x0.2 mL $1,810/3x0.2 mL $2,340/4x0.2 mL $2,855/5x0.2 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Figure 9. IF analysis of SARS2 using anti-SARS2 antibody (AAA19598).SARS2 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL rabbit anti-SARS2 Antibody (AAA19598) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 8. IHC analysis of SARS2 using anti-SARS2 antibody (AAA19598).SARS2 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-SARS2 Antibody (AAA19598) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of SARS2 using anti-SARS2 antibody (AAA19598).SARS2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-SARS2 Antibody (AAA19598) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of SARS2 using anti-SARS2 antibody (AAA19598).SARS2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-SARS2 Antibody (AAA19598) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of SARS2 using anti-SARS2 antibody (AAA19598).SARS2 was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-SARS2 Antibody (AAA19598) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of SARS2 using anti-SARS2 antibody (AAA19598).SARS2 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-SARS2 Antibody (AAA19598) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of SARS2 using anti-SARS2 antibody (AAA19598).SARS2 was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-SARS2 Antibody (AAA19598) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of SARS2 using anti-SARS2 antibody (AAA19598).SARS2 was detected in a paraffin-embedded section of human prostate adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-SARS2 Antibody (AAA19598) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of SARS2 using anti-SARS2 antibody (AAA19598).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human HepG2 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SARS2 antigen affinity purified polyclonal antibody (#AAA19598) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SARS2 at approximately 53 kDa. The expected band size for SARS2 is at 58 kDa.)

Compare (5 max)

SARS2, Polyclonal Antibody (Cat# AAA19598)

Full Name

Anti-SARS2 Antibody Picoband

Gene Names

SARS2; SYS; SARS; SERS; SARSM; SerRS; SerRSmt; mtSerRS

Reactivity

Human

Applications

WB, IHC, ICC, IF, EIA

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

< >

<< Previous Next >>

WB (Western Blot) (WB Suggested Anti-Clcn5 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: Mouse Brain)

WB (Western Blot) (Host: RabbitTarget Name: Clcn5Sample Type: Rat MuscleAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: Clcn5Sample Type: Rat LungAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: Clcn5Sample Type: Rat LiverAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: Clcn5Sample Type: Rat BrainAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: Clcn5Sample Type: Mouse SpleenAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: Clcn5Sample Type: Mouse LiverAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: Clcn5Sample Type: Mouse KidneyAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: Clcn5Sample Type: Mouse HeartAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: Clcn5Sample Type: Mouse BrainAntibody Dilution: 1.0ug/ml)

Compare (5 max)

Clcn5, Polyclonal Antibody (Cat# AAA23438)

Full Name

Clcn5 antibody - middle region

Gene Names

Clcn5; Clc5; ClC-5; Sfc13; Clc4-1; T25545; Clcn4-1; DXImx42e; 5430408K11Rik; D930009B12Rik

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish

Applications

WB

Purity

Affinity Purified

Pricing

$530/0.1 mL $1,005/2x0.1 mL $1,455/3x0.1 mL $1,885/4x0.1 mL $2,140/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells using EEF1B2 antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using EEF1B2 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using EEF1B2 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat spinal cord using EEF1B2 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using EEF1B2 Antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using EEF1B2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

Compare (5 max)

EEF1B2, Polyclonal Antibody (Cat# AAA28114)

Full Name

EEF1B2 Polyclonal Antibody

Gene Names

EEF1B2; EF1B; EEF1B; EEF1B1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

Purity

Affinity Purification

Pricing

$285/0.02 mL $500/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,780/4x0.1 mL $1,960/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistchemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

Application Data (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

Compare (5 max)

MDM2, Polyclonal Antibody (Cat# AAA29169)

Full Name

MDM2 Polyclonal Antibody

Gene Names

MDM2; HDMX; hdm2; ACTFS

Reactivity

Human

Applications

WB, IHC-P, IF

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot)

IHC (Immunohistochemistry)

IHC (Immunohistochemistry)

IHC (Immunohistchemistry)

IHC (Immunohistochemistry)

IHC (Immunohistochemistry)

IHC (Immunohistochemistry)

IHC (Immunohistochemistry)

IHC (Immunohistochemistry)

Compare (5 max)

CEACAM5, Polyclonal Antibody (Cat# AAA19192)

Full Name

Anti-CEACAM5 Antibody

Gene Names

CEACAM5; CEA; CD66e

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, EIA

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

< >

<< Previous Next >>

IF (Immunofluorescence) (Figure 8. IF analysis of PLD3 using anti-PLD3 antibody (AAA19440).PLD3 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL rabbit anti-PLD3 Antibody (AAA19440) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of PLD3 using anti-PLD3 antibody (AAA19440).PLD3 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-PLD3 Antibody (AAA19440) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of PLD3 using anti-PLD3 antibody (AAA19440).PLD3 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-PLD3 Antibody (AAA19440) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of PLD3 using anti-PLD3 antibody (AAA19440).PLD3 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-PLD3 Antibody (AAA19440) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of PLD3 using anti-PLD3 antibody (AAA19440).PLD3 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-PLD3 Antibody (AAA19440) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of PLD3 using anti-PLD3 antibody (AAA19440).PLD3 was detected in a paraffin-embedded section of human appendiceal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-PLD3 Antibody (AAA19440) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of PLD3 using anti-PLD3 antibody (AAA19440).PLD3 was detected in a paraffin-embedded section of human appendiceal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-PLD3 Antibody (AAA19440) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of PLD3 using anti-PLD3 antibody (AAA19440).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: rat brain tissue lysates,Lane 2: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLD3 antigen affinity purified polyclonal antibody (#AAA19440) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PLD3 at approximately 55 kDa. The expected band size for PLD3 is at 55 kDa.)

Compare (5 max)

PLD3, Polyclonal Antibody (Cat# AAA19440)

Full Name

Anti-PLD3 Antibody Picoband

Gene Names

PLD3; AD19; HUK4; HU-K4; SCA46

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ICC, IF, EIA

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

< >

<< Previous Next >>

WB (Western Blot) (WB Suggested Anti-PRDM9 Antibody Titration: 1 ug/mlPositive Control: 293T cells lysate)

WB (Western Blot) (Host: RabbitTarget Name: PRDM9Sample Type: MCF7Antibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: PRDM9Sample Type: HepG2Antibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: PRDM9Sample Type: 293TAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: PRDM9Sample Tissue: Human 786-0 Whole CellAntibody Dilution: 1ug/ml)

IHC (Immunohistochemistry) (Rabbit Anti-PRDM9 AntibodyParaffin Embedded Tissue: Human PlacentaAntibody Concentration: 5 ug/ml)

IHC (Immunohistochemistry) (Sample Type: Human TestisAnti-PRDM9 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

IHC (Immunohistochemistry) (Sample Type: Human TestisAnti-PRDM9 antibody IHC staining of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Compare (5 max)

PRDM9, Polyclonal Antibody (Cat# AAA23451)

Full Name

PRDM9 antibody - middle region

Gene Names

PRDM9; PFM6; KMT8B; MSBP3; ZNF899; MEISETZ

Reactivity

Human

Applications

IHC, WB

Purity

Affinity Purified

Pricing

$530/0.1 mL $1,005/2x0.1 mL $1,455/3x0.1 mL $1,885/4x0.1 mL $2,140/5x0.1 mL

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of MCF-7 cells using anti-NUP37 antibody (AAA19966).Overlay histogram showing MCF-7 cells stained with AAA19966 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NUP37 Antibody (AAA19966, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 6. IF analysis of NUP37 using anti-NUP37 antibody (AAA19966).NUP37 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NUP37 Antibody (AAA19966) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NUP37 Antibody (AAA19966) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NUP37 Antibody (AAA19966) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NUP37 Antibody (AAA19966) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HepG2 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUP37 antigen affinity purified polyclonal antibody (#AAA19966) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NUP37 at approximately 37 kDa. The expected band size for NUP37 is at 37 kDa.)

Compare (5 max)

NUP37, Polyclonal Antibody (Cat# AAA19966)

Full Name

Anti-NUP37 Antibody Picoband

Gene Names

NUP37; p37

Reactivity

Human, Rat

Applications

EIA, IF, IHC, ICC, WB, FC/FACS

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 13. Flow Cytometry analysis of MCF-7 cells using anti-EHD3 antibody (AAA19295).Overlay histogram showing MCF-7 cells stained with AAA19295 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EHD3 Antibody (AAA19295, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 12. IF analysis of EHD3 using anti- EHD3 antibody (AAA19295).EHD3 was detected in immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- EHD3 Antibody (AAA19295) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 11. IHC analysis of EHD3 using anti-EHD3 antibody (AAA19295).EHD3 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-EHD3 Antibody (AAA19295) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 10. IHC analysis of EHD3 using anti-EHD3 antibody (AAA19295).EHD3 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-EHD3 Antibody (AAA19295) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistchemistry) (Figure 9. IHC analysis of EHD3 using anti-EHD3 antibody (AAA19295).EHD3 was detected in paraffin-embedded section of human gastric adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-EHD3 Antibody (AAA19295) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 8. IHC analysis of EHD3 using anti-EHD3 antibody (AAA19295).EHD3 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-EHD3 Antibody (AAA19295) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of EHD3 using anti-EHD3 antibody (AAA19295).EHD3 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-EHD3 Antibody (AAA19295) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of EHD3 using anti-EHD3 antibody (AAA19295).EHD3 was detected in paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-EHD3 Antibody (AAA19295) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of EHD3 using anti-EHD3 antibody (AAA19295).EHD3 was detected in paraffin-embedded section of human renal clear cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-EHD3 Antibody (AAA19295) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of EHD3 using anti-EHD3 antibody (AAA19295).EHD3 was detected in paraffin-embedded section of human ovarian serous adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-EHD3 Antibody (AAA19295) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of EHD3 using anti-EHD3 antibody (AAA19295).EHD3 was detected in paraffin-embedded section of human ovarian serous adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-EHD3 Antibody (AAA19295) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

WB (Western Blot) (Figure 2. Western blot analysis of EHD3 using anti-EHD3 antibody (AAA19295).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: rat heart tissue lysatesLane 2: rat brain tissue lysatesLane 3: rat kidney tissue lysatesLane 4: rat liver tissue lysatesLane 5: mouse heart tissue lysatesLane 6: mouse brain tissue lysatesLane 7: mouse kidney tissue lysatesLane 8: mouse liver tissue lysates,After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-EHD3 antigen affinity purified polyclonal antibody (Catalog # AAA19295) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for EHD3 at approximately 70KD. The expected band size for EHD3 is at 70KD.)

WB (Western Blot) (Figure 1. Western blot analysis of EHD3 using anti-EHD3 antibody (AAA19295).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human placenta tissue lysatesLane 2: human A549 whole cell lysatesLane 3: human Hela whole cell lysatesLane 4: human CCRF-CEM whole cell lysatesLane 5: human U-87MG whole cell lysatesLane 6: human SY-SY5Y whole cell lysatesLane 7: monkey heart tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-EHD3 antigen affinity purified polyclonal antibody (Catalog # AAA19295) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for EHD3 at approximately 70KD. The expected band size for EHD3 is at 70KD.)

Compare (5 max)

EHD3, Polyclonal Antibody (Cat# AAA19295)

Full Name

Anti-EHD3 Antibody

Gene Names

EHD2; PAST2

Reactivity

Human, Monkey, Mouse, Rat

Applications

WB, IHC-P, ICC, IF, FC/FACS/FCM

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

< >

<< Previous Next >>

IHC (Immunohistchemistry) (EPDR/EPDR1 Antibody-Immunohistochemistry of paraffin-embedded Human liver cancer using EPDR1 Polyclonal Antibody at dilution of 1:40.)

IHC (Immunohistochemistry) (EPDR/EPDR1 Antibody-Immunohistochemistry of paraffin-embedded Human thyroid cancer using EPDR1 Polyclonal Antibody at dilution of 1:40.)

IHC (Immunohistochemistry) (EPDR/EPDR1 Antibody-Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (EPDR/EPDR1 Antibody-Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (EPDR/EPDR1 Antibody-Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE))

WB (Western Blot) (EPDR/EPDR1 Antibody-Western blot analysis of Mouse brain and human fetal muscle tissue, using EPDR1 Polyclonal Antibody at dilution of 1:850.)

Compare (5 max)

EPDR/EPDR1, Polyclonal Antibody (Cat# AAA21382)

Full Name

EPDR/EPDR1 Rabbit anti-Human Polyclonal Antibody

Reactivity

Mouse, Rat, Human

Applications

EIA, IHC-P, WB

Purity

Affinity purification

Pricing

$690/0.6 mL $1,325/2x0.6 mL $1,915/3x0.6 mL $2,475/4x0.6 mL $2,800/5x0.06 mL

< >

<< Previous Next >>

Application Data (Dilution: WB 1:1000-2000, IHC 1:100-200)

Application Data (Dilution: WB 1:1000-2000, IHC 1:100-200)

Application Data (Dilution: WB 1:1000-2000, IHC 1:100-200)

Application Data (Dilution: WB 1:1000-2000, IHC 1:100-200)

Application Data (Dilution: WB 1:1000-2000, IHC 1:100-200)

WB (Western Blot) (Dilution: WB 1:1000-2000, IHC 1:100-200)

Compare (5 max)

Ghrelin Receptor, Polyclonal Antibody (Cat# AAA28900)

Full Name

Ghrelin Receptor Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Figure 1. Western blot analysis of CISD2 using anti-CISD2 antibody (AAA19311).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HEK293 whole cell lysatesLane 2: human HELA whole cell lysatesLane 3: human MCF-7 whole cell lysatesLane 4: monkey kidney tissue lysatesLane 5: human SW620 whole cell lysatesLane 6: human Raji whole cell lysatesLane 7: rat kidney tissue lysatesLane 8: mouse kidney tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-CISD2 antigen affinity purified polyclonal antibody (Catalog # AAA19311) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for CISD2 at approximately 15KD. The expected band size for CISD2 is at 15KD.)

FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of U20S cells using anti-CISD2 antibody (AAA19311).Overlay histogram showing U20S cells stained with AAA19311 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CISD2 Antibody (AAA19311,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 5. IF analysis of CISD2 using anti-CISD2 antibody (AAA19311).CISD2 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-CISD2 Antibody (AAA19311) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of CISD2 using anti-CISD2 antibody (AAA19311).CISD2 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-CISD2 Antibody (AAA19311) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of CISD2 using anti-CISD2 antibody (AAA19311).CISD2 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-CISD2 Antibody (AAA19311) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of CISD2 using anti-CISD2 antibody (AAA19311).CISD2 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-CISD2 Antibody (AAA19311) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

Compare (5 max)

CISD2, Polyclonal Antibody (Cat# AAA19311)

Full Name

Anti-CISD2 Antibody

Gene Names

CISD2; ERIS; WFS2; ZCD2; NAF-1; Miner1

Reactivity

Human, Mouse, Rat, Monkey

Applications

WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of U87 cells using anti-GNG2 antibody (AAA19312).Overlay histogram showing U87 cells stained with AAA19312 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GNG2 Antibody (AAA19312, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of HEPA1-6 cells using anti-GNG2 antibody (AAA19312).Overlay histogram showing HEPA1-6 cells stained with AAA19312 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GNG2 Antibody (AAA19312, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 8. IHC analysis of GNG2 using anti-GNG2 antibody (AAA19312).GNG2 was detected in paraffin-embedded section of human renal carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-GNG2 Antibody (AAA19312) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of GNG2 using anti-GNG2 antibody (AAA19312).GNG2 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-GNG2 Antibody (AAA19312) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of GNG2 using anti-GNG2 antibody (AAA19312).GNG2 was detected in paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-GNG2 Antibody (AAA19312) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of GNG2 using anti-GNG2 antibody (AAA19312).GNG2 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-GNG2 Antibody (AAA19312) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of GNG2 using anti-GNG2 antibody (AAA19312).GNG2 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-GNG2 Antibody (AAA19312) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of GNG2 using anti-GNG2 antibody (AAA19312).GNG2 was detected in paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-GNG2 Antibody (AAA19312) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of GNG2 using anti-GNG2 antibody (AAA19312).GNG2 was detected in paraffin-embedded section of human appendicitis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-GNG2 Antibody (AAA19312) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of GNG2 using anti-GNG2 antibody (AAA19312).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human placenta tissue lysatesLane 2: human Jurkat whole cell lysatesLane 3: human SH-SY5Y whole cell lysatesLane 4: human U87 whole cell lysatesLane 5: human PC-3 whole cell lysatesLane 6: human HL-60 whole cell lysatesLane 7: rat brain tissue lysatesLane 8: rat C6 whole cell lysatesLane 9: mouse brain tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-GNG2 antigen affinity purified polyclonal antibody (Catalog # AAA19312) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for GNG2 at approximately 12KD. The expected band size for GNG2 is at 12KD.)

Compare (5 max)

GNG2, Polyclonal Antibody (Cat# AAA19312)

Full Name

Anti-GNG2 Antibody

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, FC/FACS/FCM, EIA

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

< >

<< Previous Next >>

WB (Western Blot) (WB Suggested Anti-GJA4 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: 721_B cell lysateGJA4 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells)

WB (Western Blot) (Host: RabbitTarget Name: GJA4Sample Type: MCF7Antibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: GJA4Sample Type: JurkatAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: GJA4Sample Type: HelaAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: GJA4Sample Type: 721_BAntibody Dilution: 1.0ug/ml)

IHC (Immunohistochemistry) (Rabbit Anti-GJA4 AntibodyFormalin Fixed Paraffin Embedded Tissue: Human Lung TissueObserved Staining: Membrane in alveolar type I cells and macrophagesPrimary Antibody Concentration: 1:100Other Working Concentrations: 1/600Secondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20XExposure Time: 0.5 - 2.0 sec)

Compare (5 max)

GJA4, Polyclonal Antibody (Cat# AAA23457)

Full Name

GJA4 antibody - middle region

Gene Names

GJA4; CX37

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Sheep

Applications

IHC, WB

Purity

Affinity Purified

Pricing

$530/0.1 mL $1,005/2x0.1 mL $1,455/3x0.1 mL $1,885/4x0.1 mL $2,140/5x0.1 mL

< >

<< Previous Next >>

IP (Immunoprecipitation) (Figure 6. Immunoprecipitating RPL27 in Hela whole cell lysate .Western blot analysis of RPL27 using anti-RPL27 antibody (AAA19932).Lane 1: Hela whole cell lysates (30ug)Lane 2: Rabbit control IgG instead of anti-RPL27 antibody in Hela whole cell lysate.Lane 3: anti-RPL27 antibody (2ug) + Hela whole cell lysate (500ug)After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RPL27 antigen affinity purified polyclonal antibody (AAA19932) at a dilution of 0.5ug/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody ( cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPL27 Antibody (AAA19932, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

FCM (Flow Cytometry) (Figure 4. Flow Cytometry analysis of NIH/3T3 cells using anti-RPL27 antibody (AAA19932).Overlay histogram showing NIH/3T3 cells stained with AAA19932 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPL27 Antibody (AAA19932, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

FCM (Flow Cytometry) (Figure 3. Flow Cytometry analysis of CACO-2 cells using anti-RPL27 antibody (AAA19932).Overlay histogram showing CACO-2 cells stained with AAA19932 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPL27 Antibody (AAA19932, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

WB (Western Blot) (Figure 2. Western blot analysis of RPL27 using anti-RPL27 antibody (AAA19932).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: rat liver tissue lysates,Lane 2: rat brain tissue lysates,Lane 3: rat thymus tissue lysates,Lane 4: rat RH35 whole cell lysates,Lane 5: mouse liver tissue lysates,Lane 6: mouse brain tissue lysates,Lane 7: mouse thymus tissue lysates,Lane 8: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPL27 antigen affinity purified polyclonal antibody (#AAA19932) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Lane 2: human 293T whole cell lysates,Lane 3: human HepG2 whole cell lysates,Lane 4: human Caco-2 whole cell lysates,Lane 5: human RT4 whole cell lysates,Lane 6: human Hela whole cell lysates,Lane 8: human K562 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPL27 antigen affinity purified polyclonal antibody (#AAA19932) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RPL27 at approximately 16 kDa. The expected band size for RPL27 is at 16 kDa.)

Compare (5 max)

RPL27, Polyclonal Antibody (Cat# AAA19932)

Full Name

Anti-RPL27 Antibody Picoband

Gene Names

RPL27; L27

Reactivity

Human, Mouse, Rat

Applications

WB, IP, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of A431 cells using anti-AKAP 95/AKAP8 antibody (AAA19538).Overlay histogram showing A431 cells stained with AAA19538 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AKAP 95/AKAP8 Antibody (AAA19538, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 7. IF analysis of AKAP 95/AKAP8 using anti-AKAP 95/AKAP8 antibody (AAA19538).AKAP 95/AKAP8 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL rabbit anti-AKAP 95/AKAP8 Antibody (AAA19538) overnight at 4 degree C. DyLight594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of AKAP 95/AKAP8 using anti-AKAP 95/AKAP8 antibody (AAA19538).AKAP 95/AKAP8 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-AKAP 95/AKAP8 Antibody (AAA19538) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of AKAP 95/AKAP8 using anti-AKAP 95/AKAP8 antibody (AAA19538).AKAP 95/AKAP8 was detected in a paraffin-embedded section of human gastric adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-AKAP 95/AKAP8 Antibody (AAA19538) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of AKAP 95/AKAP8 using anti-AKAP 95/AKAP8 antibody (AAA19538).AKAP 95/AKAP8 was detected in a paraffin-embedded section of human gall bladder adenosquamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-AKAP 95/AKAP8 Antibody (AAA19538) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of AKAP 95/AKAP8 using anti-AKAP 95/AKAP8 antibody (AAA19538).AKAP 95/AKAP8 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-AKAP 95/AKAP8 Antibody (AAA19538) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of AKAP 95/AKAP8 using anti-AKAP 95/AKAP8 antibody (AAA19538).AKAP 95/AKAP8 was detected in a paraffin-embedded section of human appendiceal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-AKAP 95/AKAP8 Antibody (AAA19538) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of AKAP 95/AKAP8 using anti-AKAP 95/AKAP8 antibody (AAA19538).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human HEK293 whole cell lysates,Lane 2: human PC-3 whole cell lysates,Lane 3: human HepG2 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKAP 95/AKAP8 antigen affinity purified polyclonal antibody (#AAA19538) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for AKAP 95/AKAP8 at approximately 92 kDa. The expected band size for AKAP 95/AKAP8 is at 76 kDa.)

Compare (5 max)

AKAP 95/AKAP8, Polyclonal Antibody (Cat# AAA19538)

Full Name

Anti-AKAP 95/AKAP8 Antibody Picoband

Gene Names

AKAP8; AKAP-8; AKAP95; AKAP 95; AKAP-95

Reactivity

Human

Applications

WB, IHC, ICC, IF, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of HepG2 cells using anti-HSPA1L antibody (AAA19810).Overlay histogram showing HepG2 cells stained with AAA19810 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HSPA1L Antibody (AAA19810, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 6. IF analysis of HSPA1L using anti-HSPA1L antibody (AAA19810).HSPA1L was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HSPA1L Antibody (AAA19810) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HSPA1L Antibody (AAA19810) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HSPA1L Antibody (AAA19810) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HSPA1L Antibody (AAA19810) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HepG2 whole cell lysates,Lane 3: human MCF-7 whole cell lysates,Lane 4: human 293T whole cell lysates,Lane 5: rat liver tissue lysates,Lane 6: rat testis tissue lysates,Lane 7: mouse liver tissue lysates,Lane 8: mouse testis tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPA1L antigen affinity purified polyclonal antibody (#AAA19810) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HSPA1L at approximately 70 kDa. The expected band size for HSPA1L is at 70 kDa.)

Compare (5 max)

HSPA1L, Polyclonal Antibody (Cat# AAA19810)

Full Name

Anti-HSPA1L Antibody Picoband

Gene Names

HSPA1L; HSP70T; hum70t; HSP70-1L; HSP70-HOM

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ICC, IF, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

< >

<< Previous Next >>

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded Mouse brain using BRSK1 Polyclonal Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Mouse testis using BRSK1 Polyclonal Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Mouse liver using BRSK1 Polyclonal Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human stomach using BRSK1 Polyclonal Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Rat brain using BRSK1 Polyclonal Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Rat liver using BRSK1 Polyclonal Antibody at dilution of 1:100 (40x lens).)

Compare (5 max)

BRSK1, Polyclonal Antibody (Cat# AAA22210)

Full Name

BRSK1 Polyclonal Antibody

Gene Names

BRSK1; hSAD1

Reactivity

Human, Mouse, Rat

Applications

IHC

Purity

Affinity purification

Pricing

$325/0.06 mL $435/0.12 mL $655/0.2 mL $1,245/2x0.2 mL $1,810/3x0.2 mL $2,340/4x0.2 mL $2,855/5x0.2 mL

< >

<< Previous Next >>

WB (Western Blot) (WB Suggested Anti-TGFB1I1 Antibody Titration: 0.2-1 ug/mlPositive Control: Human Lung)

WB (Western Blot) (Host: RabbitTarget Name: TGFB1I1Sample Type: Human Fetal MuscleAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: TGFB1I1Sample Type: Human Fetal LungAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: TGFB1I1Sample Type: Human Fetal HeartAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: TGFB1I1Sample Type: Human Fetal HeartAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: TGFB1I1Sample Type: Human Adult PlacentaAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: TGFB1I1Sample Type: Human Adult PlacentaAntibody Dilution: 1.0ug/ml)

IHC (Immunohistochemistry) (Human Lung)

Compare (5 max)

TGFB1I1, Polyclonal Antibody (Cat# AAA23425)

Full Name

TGFB1I1 Antibody - middle region

Gene Names

TGFB1I1; HIC5; ARA55; HIC-5; TSC-5

Reactivity

Cow, Dog, Goat, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat

Applications

IHC, WB

Purity

Affinity Purified

Pricing

$530/0.1 mL $1,005/2x0.1 mL $1,455/3x0.1 mL $1,885/4x0.1 mL $2,140/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Detection of human FOX2/RBM9 by western blot. Samples: Whole cell lysate (10 ug) from U2OS, HEK293T, Hep-G2, HeLa, and GaMG cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RBM9 antibody (AAA23776 lot 4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)

WB (Western Blot) (Detection of human FOX2/RBM9 by western blot. Samples: Whole cell lysate (10 ug) from NIH 3T3, TCMK-1, CT26, and C6 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RBM9 antibody AAA23776 (lot AAA23776-4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)

IP (Immunoprecipitation) (Detection of human FOX2/RBM9 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RBM9 antibody (AAA23776 lot 4) used for IP at 6 ug per reaction. RBM9 was also immunoprecipitated by a previous lot of this antibody (AAA23776 lot 3) and a second antibody to a different epitope of RBM9 (BL2440). For blotting immunoprecipitated RBM9, AAA23776 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)

IHC (Immunohistochemistry) (Detection of human FOX2/RBM9 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-RBM9 (AAA23776 Lot 4) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)

FCM (Flow Cytometry) (Detection of human RBM9 (shaded) in HeLa cells by flow cytometry. Antibody: Rabbit anti-RBM9 polyclonal antibody (AAA23776)) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

FCM (Flow Cytometry) (Detection of mouse RBM9 (shaded) in EL4 cells by flow cytometry. Antibody: Rabbit anti-RBM9 polyclonal antibody (AAA23776)) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

Compare (5 max)

FOX2/RBM9, Polyclonal Antibody (Cat# AAA23776)

Full Name

Rabbit anti-FOX2/RBM9 Antibody, Affinity Purified

Gene Names

RBFOX2; RTA; fxh; FOX2; RBM9; Fox-2; HNRBP2; HRNBP2; dJ106I20.3

Reactivity

Human, Mouse, Rat

Applications

WB, IP, IHC, FC/FACS

Purity

Antigen Affinity Purified

Pricing

$290/0.01 mg $630/0.1 mg $965/5x0.01 mg $1,200/2x0.1 mg $1,735/3x0.1 mg $2,245/4x0.1 mg $2,500/5x0.1 mg

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of MCF7 cell using PIN1 antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat spleen using PIN1 antibody at dilution of 1:100 (400x lens).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat lung using PIN1 antibody at dilution of 1:100 (400x lens).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat kidney using PIN1 antibody at dilution of 1:100 (400x lens).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human esophageal cancer using PIN1 antibody at dilution of 1:100 (400x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using PIN1 antibody.)

Compare (5 max)

PIN1, Polyclonal Antibody (Cat# AAA29684)

Full Name

PIN1 Antibody

Gene Names

PIN1; DOD; UBL5

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

Purity

Antibodies were purified by affinity purification using immunogen.

Pricing

$360/0.05 mL $450/0.1 mL $855/2x0.1 mL $1,230/3x0.1 mL $1,595/4x0.1 mL $1,750/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Figure 7. IF analysis of METTL1 using anti- METTL1 antibody (AAA19336).METTL1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-METTL1 Antibody (AAA19336) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of METTL1 using anti-METTL1 antibody (AAA19336).METTL1 was detected in paraffin-embedded section of human skin cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-METTL1 Antibody (AAA19336) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of METTL1 using anti-METTL1 antibody (AAA19336).METTL1 was detected in paraffin-embedded section of human pancreatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-METTL1 Antibody (AAA19336) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of METTL1 using anti-METTL1 antibody (AAA19336).METTL1 was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-METTL1 Antibody (AAA19336) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of METTL1 using anti-METTL1 antibody (AAA19336).METTL1 was detected in paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-METTL1 Antibody (AAA19336) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of METTL1 using anti-METTL1 antibody (AAA19336).METTL1 was detected in paraffin-embedded section of rat pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-METTL1 Antibody (AAA19336) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of METTL1 using anti-METTL1 antibody (AAA19336).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HELA whole cell lysatesLane 2: human Jurkat whole cell lysatesLane 3: human HEK293 whole cell lysatesLane 4: human A549 whole cell lysatesLane 5: human MCF-7 whole cell lysatesLane 6: human PC-3 whole cell lysatesLane 7: human T-47D whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-METTL1 antigen affinity purified polyclonal antibody (Catalog # AAA19336) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for METTL1 at approximately 34KD. The expected band size for METTL1 is at 34KD.)

Compare (5 max)

METTL1, Polyclonal Antibody (Cat# AAA19336)

Full Name

Anti-METTL1 Antibody

Gene Names

METTL1; TRM8; TRMT8; C12orf1; YDL201w

Reactivity

Human, Rat

Applications

WB, IHC-P, ICC, IF, EIA

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

< >

<< Previous Next >>

IHC (Immunohistochemistry) (DAB staining on IHC-P;Samples: Mouse Stomach Tissue;Primary Ab: 20ug/ml Rabbit Anti-Mouse DGKz AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Samples: Mouse Pancreas Tissue;Primary Ab: 20ug/ml Rabbit Anti-Mouse DGKzAntibody Second Ab: 2ug/mL HRP Linked Caprine Anti-Rabbit IgGPolyclonal Antibody)

WB (Western Blot) (Western Blot; Sample: Recombinant DGKz, Mouse.)

Compare (5 max)

Diacylglycerol Kinase Zeta, Polyclonal Antibody (Cat# AAA20882)

Full Name

Polyclonal Antibody to Diacylglycerol Kinase Zeta (DGKz)

Gene Names

Dgkz; mDGK[z]; E130307B02Rik; F730209L11Rik

Reactivity

Mouse, Human, Rat

Applications

WB, IHC, ICC, IP

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

$245/0.1 mL $310/0.2 mL $475/0.5 mL $575/1 mL $1,515/5 mL $2,935/2x5 mL $4,275/3x5 mL $5,540/4x5 mL $6,750/5x5 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells using ASPSCR1 antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using ASPSCR1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse lung using ASPSCR1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human kidney cancer using ASPSCR1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using ASPSCR1 antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using ASPSCR1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)

Compare (5 max)

ASPSCR1, Polyclonal Antibody (Cat# AAA28119)

Full Name

ASPSCR1

Gene Names

ASPSCR1; TUG; ASPL; ASPS; RCC17; UBXD9; UBXN9; ASPCR1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 11. Flow Cytometry analysis of HepG2 cells using anti-PELI3 antibody (AAA19930).Overlay histogram showing HepG2 cells stained with AAA19930 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PELI3 Antibody (AAA19930, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 10. IF analysis of PELI3 using anti-PELI3 antibody (AAA19930).PELI3 was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PELI3 Antibody (AAA19930) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PELI3 Antibody (AAA19930) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PELI3 Antibody (AAA19930) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PELI3 Antibody (AAA19930) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PELI3 Antibody (AAA19930) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PELI3 Antibody (AAA19930) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PELI3 Antibody (AAA19930) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PELI3 Antibody (AAA19930) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: rat heart tissue lysates,Lane 3: rat testis tissue lysates,Lane 4: mouse brain tissue lysates,Lane 5: mouse heart tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PELI3 antigen affinity purified polyclonal antibody (#AAA19930) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PELI3 at approximately 51 kDa. The expected band size for PELI3 is at 51 kDa.)

Compare (5 max)

PELI3, Polyclonal Antibody (Cat# AAA19930)

Full Name

Anti-PELI3 Antibody Picoband

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ICC, IF, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

Compare (5 max)

CRP, Polyclonal Antibody (Cat# AAA29174)

Full Name

CRP Polyclonal Antibody

Gene Names

CRP; PTX1

Reactivity

Human, Rat

Applications

WB, IHC-P

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of HEL cells using anti-STXBP6 antibody (AAA19956).Overlay histogram showing HEL cells stained with AAA19956 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STXBP6 Antibody (AAA19956, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of STXBP6 using anti-STXBP6 antibody (AAA19956).STXBP6 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STXBP6 Antibody (AAA19956) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STXBP6 Antibody (AAA19956) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STXBP6 Antibody (AAA19956) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STXBP6 Antibody (AAA19956) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Caco-2 whole cell lysates,Lane 3: human Ramos whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STXBP6 antigen affinity purified polyclonal antibody (#AAA19956) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for STXBP6 at approximately 26 kDa. The expected band size for STXBP6 is at 24 kDa.)

Compare (5 max)

STXBP6, Polyclonal Antibody (Cat# AAA19956)

Full Name

Anti-STXBP6 Antibody Picoband

Gene Names

STXBP6; amisyn; HSPC156

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

< >

<< Previous Next >>

WB (Western Blot) (WB Suggested Anti-ACTR1B Antibody Titration: 0.2-1 ug/mlPositive Control: HepG2 cell lysateACTR1B is supported by BioGPS gene expression data to be expressed in HepG2)

WB (Western Blot) (Host: RabbitTarget Name: ACTR1BSample Type: Human Fetal LungAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: ACTR1BSample Type: Human Fetal LiverAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: ACTR1BSample Type: Human Fetal HeartAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: ACTR1BSample Type: Human Fetal BrainAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: ACTR1BSample Type: Human 721_BAntibody Dilution: 1.0ug/mlACTR1B is supported by BioGPS gene expression data to be expressed in 721_B)

Compare (5 max)

ACTR1B, Polyclonal Antibody (Cat# AAA23545)

Full Name

ACTR1B antibody - C-terminal region

Gene Names

ACTR1B; PC3; ARP1B; CTRN2

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish

Applications

WB

Purity

Affinity Purified

Pricing

$530/0.1 mL $1,005/2x0.1 mL $1,455/3x0.1 mL $1,885/4x0.1 mL $2,140/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (25 ug of the indicated Human whole cell extracts was loaded onto a 10-20% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment. Canonical 15 kDa isoform is identified, and a second isoform of 18 kDa is also present in some samples.)

WB (Western Blot) (WB Suggested Anti-PFN1 Antibody Titration: 0.2-1 ug/mlPositive Control: Human Liver)

WB (Western Blot) (Lanes:1. 20 ug murine non-pregnant uteria tissue 2. 20 ug murine non-laboring uterine tissue 3. 20 ug murine laboring uterine tissue 4. 20 ug murine preterm laboring uterine tissuePrimary Antibody Dilution:1:2000Secondary Antibody:Goat anti-rabbit-Alexa Fluor 680Secondary Antibody Dilution:1:25,000Gene Name:PFN1Submitted by:Anonymous)

WB (Western Blot) (Lanes:1. 20 ug human pregnant uterine muscle cells + hormone 2. 20 ug human pregnant uterine muscle cells - hormonePrimary Antibody Dilution:1:2000Secondary Antibody:Goat anti-rabbit-Alexa Fluor 680Secondary Antibody Dilution:1:25,000Gene Name:PFN1Submitted by:Anonymous)

IHC (Immunohistochemistry) (Rabbit Anti-PFN1 antibodyFormalin Fixed Paraffin Embedded Tissue: Human TonsilPrimary antibody Concentration: 1:100Secondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20xExposure Time: 0.5-2.0sec)

IHC (Immunohistochemistry) (Rabbit Anti-PFN1 antibodyFormalin Fixed Paraffin Embedded Tissue: Human SpleenPrimary antibody Concentration: 1:100Secondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20xExposure Time: 0.5-2.0sec)

IHC (Immunohistochemistry) (Rabbit Anti-PFN1 antibodyFormalin Fixed Paraffin Embedded Tissue: Human LiverPrimary antibody Concentration: 1:100Secondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20xExposure Time: 0.5-2.0sec)

Compare (5 max)

PFN1, Polyclonal Antibody (Cat# AAA23532)

Full Name

PFN1 antibody - N-terminal region

Gene Names

PFN1; ALS18

Reactivity

Tested Species Reactivity: Human, Mouse
Predicted Species Reactivity: Cow, Guinea Pig, Horse, Human, Mouse, Rat, Zebrafish

Applications

WB

Purity

Affinity Purified

Pricing

$530/0.1 mL $1,005/2x0.1 mL $1,455/3x0.1 mL $1,885/4x0.1 mL $2,140/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (WB Suggested Anti-GRSF1 Antibody Titration: 0.2-1 ug/mlPositive Control: Hela cell lysateGRSF1 is strongly supported by BioGPS gene expression data to be expressed in Human HeLa cells)

WB (Western Blot) (Host: RabbitTarget Name: GRSF1Sample Type: JurkatAntibody Dilution: 1.0ug/mlGRSF1 is strongly supported by BioGPS gene expression data to be expressed in Jurkat)

WB (Western Blot) (Host: RabbitTarget Name: GRSF1Sample Type: JurkatAntibody Dilution: 1.0ug/mlGRSF1 is strongly supported by BioGPS gene expression data to be expressed in Jurkat)

WB (Western Blot) (Host: RabbitTarget Name: GRSF1Sample Type: Human Fetal LungAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: GRSF1Sample Type: Human Fetal LiverAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: GRSF1Sample Type: Human Fetal LiverAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: GRSF1Sample Type: Human Fetal HeartAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: GRSF1Sample Type: Human Fetal BrainAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: GRSF1Sample Type: Human Fetal BrainAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: GRSF1Sample Type: HelaAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: GRSF1Sample Type: HelaAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: GRSF1Sample Tissue: Human 293TAntibody Dilution: 1.0ug/ml)

Compare (5 max)

GRSF1, Polyclonal Antibody (Cat# AAA23475)

Full Name

GRSF1 antibody - middle region

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat

Applications

WB

Purity

Affinity Purified

Pricing

$530/0.1 mL $1,005/2x0.1 mL $1,455/3x0.1 mL $1,885/4x0.1 mL $2,140/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Figure 1. Western blot analysis of Ran using anti-Ran antibody (AAA19131). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysate,Lane 2: rat testis tissue lysate,Lane 3: rat thymus tissue lysate,Lane 4: mouse brain tissue lysate,Lane 5: mouse testis tissue lysate,Lane 6: mouse thymus tissue lysate,Lane 7: human A549 whole cell lysate,Lane 8: human 22RV1 whole cell lysate,Lane 9: human Hela whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ran antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Ran at approximately 24KD. The expected band size for Ran is at 24KD.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of Ran using anti-Ran antibody (AAA19131).Ran was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Ran Antibody (AAA19131) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )

IHC (Immunohistochemistry) (Figure 2. IHC analysis of Ran using anti-Ran antibody (AAA19131).Ran was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Ran Antibody (AAA19131) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of Ran using anti-Ran antibody (AAA19131).Ran was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Ran Antibody (AAA19131) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )

IHC (Immunohistochemistry) (Figure 6. IHC analysis of Ran using anti-Ran antibody (AAA19131).Ran was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Ran Antibody (AAA19131) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )

IHC (Immunohistochemistry) (Figure 5. IHC analysis of Ran using anti-Ran antibody (AAA19131).Ran was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Ran Antibody (AAA19131) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )

Compare (5 max)

Ran, Polyclonal Antibody (Cat# AAA19131)

Full Name

Anti-Ran Picoband Antibody

Gene Names

RAN; TC4; Gsp1; ARA24

Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins.

Applications

IHC, WB

Purity

Immunogen affinity purified

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of JK cells using anti-GSPT1,2 antibody (AAA19586).Overlay histogram showing JK cells stained with AAA19586 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GSPT1,2 Antibody (AAA19586, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 5. IF analysis of GSPT1,2 using anti-GSPT1,2 antibody (PB9077).GSPT1,2 was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL rabbit anti-GSPT1,2 Antibody (PB9077) overnight at 4 degree C. DyLight594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of GSPT1,2 using anti-GSPT1,2 antibody (AAA19586).GSPT1,2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-GSPT1,2 Antibody (AAA19586) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of GSPT1,2 using anti-GSPT1,2 antibody (AAA19586).GSPT1,2 was detected in a paraffin-embedded section of human gastric adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-GSPT1,2 Antibody (AAA19586) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of GSPT1,2 using anti-GSPT1,2 antibody (AAA19586).GSPT1,2 was detected in a paraffin-embedded section of human gall bladder adenosquamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-GSPT1,2 Antibody (AAA19586) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of GSPT1,2 using anti-GSPT1,2 antibody (AAA19586).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human K562 whole cell lysates,Lane 2: human Raji whole cell lysates,Lane 3: rat pancreas tissue lysates,Lane 4: mouse pancreas tissue lysates,Lane 5: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSPT1,2 antigen affinity purified polyclonal antibody (#AAA19586) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GSPT1,2 at approximately 85 kDa. The expected band size for GSPT1,2 is at 85 kDa.)

Compare (5 max)

GSPT1,2, Polyclonal Antibody (Cat# AAA19586)

Full Name

Anti-GSPT1,2 Antibody Picoband

Gene Names

GSPT1; GST1; ETF3A; eRF3a; 551G9.2

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ICC, IF, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

< >

<< Previous Next >>

WB (Western Blot) (Host: RabbitTarget Name: MUC1Sample Type: HepG2Lane A: Primary AntibodyLane B: Primary Antibody + Blocking PeptidePrimary Antibody Concentration: 1ug/mlPeptide Concentration: 5ug/mlLysate Quantity: 25ug/lane/laneGel Concentration: 0.12)

WB (Western Blot) (Host: RabbitTarget Name: MUC1Sample Type: HepG2 Whole cell lysatesAntibody Dilution: 1.0ug/ml)

IHC (Immunohistochemistry) (Sample Type :Pig stomachPrimary Antibody Dilution :1:5000 + 1:2000Secondary Antibody :Anti-rabbit-HRPSecondary Antibody Dilution :1:1000Color/Signal Descriptions :Brown: MUC1 Blue: NucleusGene Name :MUC1Submitted by :Dr. Sara Linden, University of Gothenburg)

IHC (Immunohistochemistry) (Sample Type :Pig stomachPrimary Antibody Dilution :1:5000 + 1:2000Secondary Antibody :Anti-rabbit-HRPSecondary Antibody Dilution :1:1000Color/Signal Descriptions :Brown: MUC1 Blue: NucleusGene Name :MUC1Submitted by :Dr. Sara Linden, University of Gothenburg)

IHC (Immunohistochemistry) (Sample Type :Human stomachPrimary Antibody Dilution :1:1000Secondary Antibody :Anti-rabbit-HRPSecondary Antibody Dilution :1:5000Color/Signal Descriptions :Brown: MUC1 Blue: NucleusGene Name :MUC1Submitted by :Dr. Sara Linden, University of Gothenburg)

IHC (Immunohistochemistry) (Rabbit Anti-MUC1 AntibodyParaffin Embedded Tissue: Human KidneyCellular Data: Epithelial cells of renal tubuleAntibody Concentration: 4.0-8.0 ug/mlMagnification: 400X)

IHC (Immunohistochemistry) (NMuFG cells in ImmunohistochemistryDilutions 1:50 for the three primary antibodies and 1:100 for FITC-conjugated secondary.)

Compare (5 max)

MUC1, Polyclonal Antibody (Cat# AAA23492)

Full Name

MUC1 antibody - C-terminal region

Gene Names

MUC1; EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; CD227; H23AG; MCKD1; MUC-1; ADMCKD; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Pig

Applications

IHC, WB

Purity

Affinity Purified

Pricing

$530/0.1 mL $1,005/2x0.1 mL $1,455/3x0.1 mL $1,885/4x0.1 mL $2,140/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of L929 cells using GRSF1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using GRSF1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human esophageal cancer using GRSF1 Rabbit pAb at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Rat liver using GRSF1 Rabbit pAb at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Mouse brain using GRSF1 Rabbit pAb at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using GRSF1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 180s.)

Compare (5 max)

GRSF1, Polyclonal Antibody (Cat# AAA28367)

Full Name

GRSF1 Rabbit pAb

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

Purity

Affinity purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (WB Suggested Anti-SLC39A6 Antibody Titration: 1.25ug/mlPositive Control: Jurkat cell lysate)

WB (Western Blot) (Host: RabbitTarget Name: WT1Sample Type: 721_BAntibody Dilution: 1.0ug/mlSLC39A6 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells)

WB (Western Blot) (Host: RabbitTarget Name: NOP56Sample Type: MCF7Antibody Dilution: 1.0ug/mlSLC39A6 is strongly supported by BioGPS gene expression data to be expressed in MCF7)

WB (Western Blot) (Host: RabbitTarget Name: NOP56Sample Type: Human Fetal BrainAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: HIRIP3Sample Type: 293TAntibody Dilution: 1.0ug/mlSLC39A6 is strongly supported by BioGPS gene expression data to be expressed in HEK293T)

IHC (Immunohistochemistry) (Human kidney)

Compare (5 max)

SLC39A6, Polyclonal Antibody (Cat# AAA23513)

Full Name

SLC39A6 antibody - middle region

Gene Names

SLC39A6; ZIP6; LIV-1

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Sheep

Applications

IHC, WB

Purity

Protein A purified

Pricing

$475/0.1 mL $905/2x0.1 mL $1,310/3x0.1 mL $1,690/4x0.1 mL $1,890/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Figure 7. IHC analysis of CD105 using anti-CD105 antibody (AAA19165).CD105 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD105 Antibody (AAA19165) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of CD105 using anti-CD105 antibody (AAA19165).CD105 was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD105 Antibody (AAA19165) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of CD105 using anti-CD105 antibody (AAA19165).CD105 was detected in paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD105 Antibody (AAA19165) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of CD105 using anti-CD105 antibody (AAA19165).CD105 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD105 Antibody (AAA19165) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of CD105 using anti-CD105 antibody (AAA19165).CD105 was detected in paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD105 Antibody (AAA19165) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of CD105 using anti-CD105 antibody (AAA19165).CD105 was detected in paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD105 Antibody (AAA19165) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of CD105 using anti-CD105 antibody (AAA19165).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: mouse lung tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD105 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD105 at approximately 95-105KD. The expected band size for CD105 is at 71KD.)

Compare (5 max)

CD105, Polyclonal Antibody (Cat# AAA19165)

Full Name

Anti-CD105 Picoband Antibody

Gene Names

Eng; Endo; CD105; AI528660; AI662476; S-endoglin

Reactivity

Mouse, Rat
No cross reactivity with other proteins.

Applications

EIA, IHC, WB

Purity

Immunogen affinity purified

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

< >

<< Previous Next >>

DB (Dot Blot) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

DB (Dot Blot) (Dot blot analysis of anti-Phospho-HIST1H3B3-S10 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

WB (Western Blot) (Western blot analysis of extracts from Hela cells,untreated or treated with FBS and Calyculin A,100nM, using phospho-H3-Ps10(left) or H3 antibody(right))

WB (Western Blot) (Western blot analysis of extracts from Hela cells,untreated or treated with FBS and Calyculin A,100nM, using phospho-HIST1H3B3-S10(left) or HIST1H3B3 antibody(right))

WB (Western Blot) (Western blot analysis of lysate from Hela cell line, using Phospho-H3-pS10 Antibody. AAA28670 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)

WB (Western Blot) (Western blot analysis of lysates from Hela cell line, untreated or treated with FBS+calyculin A, 100ng/ml, using Phospho-H3-Ps10 Antibody, (upper) or Beta-actin (lower).)

WB (Western Blot) (Western blot analysis of lysates from Hela cell line, untreated or treated with Calyculin A, 100ng/ml, using Phospho-H3-pS10 Antibody (upper) or Beta-actin (lower).)

Compare (5 max)

Phospho-HIST1H3B3 (S10), Polyclonal Antibody (Cat# AAA28670)

Full Name

Phospho-HIST1H3B3 (S10) Antibody

Gene Names

HIST1H3A; H3/A; H3FA

Reactivity

Human

Applications

DB, EIA, IHC, WB

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$275/0.05 mL $510/0.2 mL $975/2x0.2 mL $1,415/3x0.2 mL $1,825/4x0.2 mL $2,070/5x0.2 mL

< >

<< Previous Next >>

WB (Western Blot) (WB Suggested Anti-SHH Antibody Titration: 0.2-1 ug/mlPositive Control: HepG2 cell lysate)

WB (Western Blot) (Host: RabbitTarget Name: SHHSample Tissue: Human Stomach TumorAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: MouseTarget Name: SHHSample Tissue: Mouse BrainAntibody Dilution: 1ug/ml)

IHC (Immunohistochemistry) (Sample Type :Human glioma cellsPrimary Antibody Dilution :1:200Secondary Antibody :Anti-rabbit-GFPSecondary Antibody Dilution :1:500Color/Signal Descriptions :1. SHH: Green 2. DAPI: Blue 3. MergeGene Name :SHHSubmitted by :ELIAS EL HABR, PhD, Post-Doctoral fellow, INSERM U894 Glial Plasticity Team, Psychiatry& Neurosciences Center, St Anne Hospital, 2 Ter, Rue d'Alesia, 75014 Paris)

IHC (Immunohistochemistry) (Rabbit Anti-SHH AntibodyFormalin Fixed Paraffin Embedded Tissue: Human Urinary Bladder TissueObserved Staining: Plasma membrane, CytoplasmPrimary Antibody Concentration: 1:100Other Working Concentrations: N/ASecondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20XExposure Time: 0.5 - 2.0 sec)

IHC (Immunohistochemistry) (Sample Type: Chicken embryosChicken embryosPrimary antibody: 1:1000 (AAA23516) anti -shhSecondary Antibody: 1:500 goat anti-rabbit HRP conjugated)

Compare (5 max)

SHH, Polyclonal Antibody (Cat# AAA23516)

Full Name

SHH antibody - N-terminal region

Gene Names

SHH; TPT; HHG1; HLP3; HPE3; SMMCI; ShhNC; TPTPS; MCOPCB5

Reactivity

Predicted Reactivity: Cow, Dog, Goat, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish, Chicken (Tested Reactivity: Human, Mouse, Chicken)

Applications

IHC, WB

Purity

Affinity Purified

Pricing

$530/0.1 mL $1,005/2x0.1 mL $1,455/3x0.1 mL $1,885/4x0.1 mL $2,140/5x0.1 mL

What are Polyclonal Antibodies?

Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).

Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.

Key Uses of Polyclonal Antibodies

• Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.


• Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.


• ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.


• Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.


• Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.

2. Rigorous Quality Control

All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.

3. Wide Assortment of Antibodies

Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.

4. Highly Purified

Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.

FAQ

1. How are polyclonal antibodies produced?

Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.

2. How do polyclonal antibodies differ from monoclonal antibodies?

Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.

3. How should I store polyclonal antibodies?

Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.