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▼Polyclonal Antibodies
At AAA Biotech also known as AAA Bio or AAABio, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
Viewing 2800-2850 of 96811 product results
IHC (Immunohistochemisry)
(Figure 3. IHC analysis of Sacsin using anti-Sacsin antibody (AAA124580).Sacsin was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Sacsin Antibody (AAA124580) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
Sacsin, Polyclonal Antibody (Cat# AAA124580)
Full Name
Anti-Sacsin Picoband antibody
Gene Names
SACS; SPAX6; ARSACS; DNAJC29; PPP1R138
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified
Pricing
WB (Western Blot)
(Figure 1. Western blot analysis of TSH Receptor using anti-TSH Receptor antibody (AAA124582).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat brain tissue lysates,Lane 2: mouse brain tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSH Receptor antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TSH Receptor at approximately 86KD. The expected band size for TSH Receptor is at 86KD.)
TSH Receptor, Polyclonal Antibody (Cat# AAA124582)
Full Name
Anti-TSH Receptor Picoband Antibody
Gene Names
TSHR; LGR3; CHNG1; hTSHR-I
Reactivity
Reacts with: Mouse, Rat
Predicted to work with: Human
Predicted to work with: Human
Applications
Western Blot
Purity
Immunogen affinity purified
Pricing
IHC (Immunohistochemistry)
(Figure 4. IHC analysis of Lactoferrin using anti-Lactoferrin antibody (AAA124585).Lactoferrin was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Lactoferrin Antibody (AAA124585) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
Lactoferrin, Polyclonal Antibody (Cat# AAA124585)
Full Name
Anti-Lactoferrin Picoband antibody
Gene Names
LTF; LF; HLF2; GIG12; HEL110
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Western Blot, Immunohistochemistry
Pricing
WB (Western Blot)
(Figure 1. Western blot analysis of PAI1 using anti-PAI1 antibody (AAA124586).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat small intestine tissue lysates,Lane 2: rat kidney tissue lysates,Lane 3: mouse kidney tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAI1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PAI1 at approximately 45-50KD. The expected band size for PAI1 is at 45KD.)
PAI1, Polyclonal Antibody (Cat# AAA124586)
Full Name
Anti-PAI1 Picoband antibody
Gene Names
Serpine1; Pai1; PAI1A; Planh; Pai1aa; RATPAI1A
Reactivity
Mouse, Rat
Applications
Direct ELISA, Western Blot
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohiostchemistry)
(Figure 2. IHC analysis of ATF6 using anti- ATF6 antibody (AAA124587).ATF6 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- ATF6 Antibody (AAA124587) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
ATF6, Polyclonal Antibody (Cat# AAA124587)
Full Name
Anti-ATF6 Picoband Antibody
Gene Names
ATF6; ACHM7; ATF6A
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins
No cross reactivity with other proteins
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified
Pricing
IHC (Immunohistochemisry)
(Figure 3. IHC analysis of Adenylosuccinate Lyase using anti-Adenylosuccinate Lyase antibody (AAA124591).Adenylosuccinate Lyase was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Adenylosuccinate Lyase Antibody (AAA124591) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
Adenylosuccinate Lyase, Polyclonal Antibody (Cat# AAA124591)
Full Name
Anti-Adenylosuccinate Lyase Picoband Antibody
Gene Names
ASL; ASAL
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified
Pricing
IHC (Immunohistochemisry)
(Figure 3. IHC analysis of PF4 using anti-PF4 antibody (AAA124596).PF4 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PF4 Antibody (AAA124596) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
PF4/Cxcl4, Polyclonal Antibody (Cat# AAA124596)
Full Name
Anti-PF4/Cxcl4 Picoband antibody
Gene Names
Pf4; PF-4; Pf4a; Cxcl4; RATPF4A
Reactivity
Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Western Blot, Immunohistochemistry
Pricing
IHC (Immunohiostchemistry)
(Figure 2. IHC analysis of GFI1 using anti-GFI1 antibody (AAA124598).GFI1 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GFI1 Antibody (AAA124598) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
GFI1, Polyclonal Antibody (Cat# AAA124598)
Full Name
Anti-GFI1 Picoband antibody
Gene Names
GFI1; SCN2; GFI-1; GFI1A; ZNF163
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Immunohistochemistry
Pricing
IHC (Immunohistochemisry)
(Figure 3. IHC analysis of CD59 using anti-CD59 antibody (AAA124602).CD59 was detected in paraffin-embedded section of mouse thymus tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD59 Antibody (AAA124602) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
CD59, Polyclonal Antibody (Cat# AAA124602)
Full Name
Anti-CD59 Picoband antibody
Gene Names
Cd59a; Cd59; AA987121; protectin
Reactivity
Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Western Blot, Immunohistochemistry
Pricing
WB (Western Blot)
(Figure 2. Western blot analysis of GNS using anti-GNS antibody (AAA124609).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat lung tissue lysates,Lane 2: rat kidney tissue lysates,Lane 3: rat testis tissue lysates,Lane 4: rat PC-12 whole cell lysates,Lane 5: mouse lung tissue lysates,Lane 6: mouse kidney tissue lysates,Lane 7: mouse testis tissue lysates,Lane 8: mouse spleen tissue lysates,Lane 9: mouse thymus tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GNS antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GNS at approximately 90KD. The expected band size for GNS is at 62KD.)
GNS, Polyclonal Antibody (Cat# AAA124609)
Full Name
Anti-GNS Picoband antibody
Gene Names
GNS; G6S
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Western Blot
Pricing
IHC (Immunohiostchemistry)
(Figure 2. IHC analysis of BCMA using anti-BCMA antibody (AAA124612).BCMA was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-BCMA Antibody (AAA124612) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
BCMA, Polyclonal Antibody (Cat# AAA124612)
Full Name
Anti-BCMA Picoband antibody
Gene Names
TNFRSF17; BCM; BCMA; CD269; TNFRSF13A
Reactivity
Human
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Western Blot, Immunohistochemistry
Pricing
FCM/FACS (Flow Cytometry)
(Figure 4. Flow Cytometry analysis of THP-1 cells using anti-DC-SIGN antibody (AAA124613).Overlay histogram showing THP-1 cells stained with AAA124613 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DC-SIGN Antibody (AAA124613,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
DC-SIGN, Polyclonal Antibody (Cat# AAA124613)
Full Name
Anti-DC-SIGN Picoband antibody
Gene Names
CD209; CDSIGN; CLEC4L; DC-SIGN; DC-SIGN1
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Western Blot, Immunohistochemistry
Pricing
IHC (Immunohistochemistry)
(Figure 5. IHC analysis of RBP4 using anti-RBP4 antibody (AAA124617).RBP4 was detected in paraffin-embedded section of rat pancreas tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-RBP4 Antibody (AAA124617) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
RBP4/Retinol Binding Protein 4, Polyclonal Antibody (Cat# AAA124617)
Full Name
Anti-RBP4/Retinol Binding Protein 4 Picoband Antibody
Gene Names
Rbp4; Rbp-4
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified
Pricing
IHC (Immunohistochemistry)
(Figure 4. IHC analysis of SDHB using anti-SDHB antibody (AAA124618).SDHB was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SDHB Antibody (AAA124618) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
SDHB, Polyclonal Antibody (Cat# AAA124618)
Full Name
Anti-SDHB Picoband antibody
Gene Names
SDHB; IP; SDH; CWS2; PGL4; SDH1; SDH2; SDHIP
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Western Blot, Immunohistochemistry
Pricing
IHC (Immunohiostchemistry)
(Figure 2. IHC analysis of IL12B using anti-IL12B antibody (AAA124625).IL12B was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-IL12B Antibody (AAA124625) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
IL12B/Il 12, Polyclonal Antibody (Cat# AAA124625)
Full Name
Anti-IL12B/Il 12 Picoband Antibody
Gene Names
Il12b; Il12
Reactivity
Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified
Pricing
IHC (Immunohistochemisry)
(Figure 3. IHC analysis of VCAM1 using anti-VCAM1 antibody (AAA124628).VCAM1 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-VCAM1 Antibody (AAA124628) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
VCAM1/Cd106, Polyclonal Antibody (Cat# AAA124628)
Full Name
Anti-VCAM1/Cd106 Picoband antibody
Gene Names
Vcam1; VCAM1B
Reactivity
Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Western Blot, Immunohistochemistry
Pricing
WB (Western Blot)
(Figure 1. Western blot analysis of MTA2 using anti-MTA2 antibody.)
MTA2/PID, Polyclonal Antibody (Cat# AAA125463)
Full Name
Anti-MTA2/PID Antibody
Gene Names
MTA2; PID; MTA1L1
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
Acetyl Coenzyme A Carboxylase/ACACB, Polyclonal Antibody (Cat# AAA125469)
Full Name
Anti-Acetyl Coenzyme A Carboxylase/ACACB Antibody
Gene Names
ACACB; ACC2; ACCB; HACC275
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Figure 1. Western blot analysis of ASIC2 using anti-ASIC2 antibody.)
ACCN1/ASIC2, Polyclonal Antibody (Cat# AAA125471)
Full Name
Anti-ACCN1/ASIC2 Antibody
Gene Names
ASIC2; ACCN; BNC1; MDEG; ACCN1; BNaC1; ASIC2a; hBNaC1
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Figure 1. Western blot analysis of CHD2 using anti-CHD2 antibody.)
CHD2, Polyclonal Antibody (Cat# AAA125472)
Full Name
Anti-CHD2 Antibody
Gene Names
CHD2; EEOC
Reactivity
Human
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Figure 1. Western blot analysis of CHRM1 using anti-CHRM1 antibody.)
Muscarinic Acetylcholine Receptor 1/CHRM1, Polyclonal Antibody (Cat# AAA125473)
Full Name
Anti-Muscarinic Acetylcholine Receptor 1/CHRM1 Antibody
Gene Names
CHRM1; M1; HM1; M1R
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
SMC4, Polyclonal Antibody (Cat# AAA125476)
Full Name
Anti-SMC4 Antibody
Gene Names
SMC4; CAPC; CAP-C; SMC-4; SMC4L1
Reactivity
Human
Applications
Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of rat spleen tissue using anti-IL10 antibody (AAA125509).Overlay histogram showing rat spleen tissue stained with AAA125509 (Blue line). The tissue section was blocked with 10% normal goat serum. And then incubated with rabbit anti-IL10 Antibody (AAA125509, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
IL10, Polyclonal Antibody (Cat# AAA125509)
Full Name
Anti-IL10 Antibody
Gene Names
Il10; IL10X
Reactivity
Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Figure 4. IF analysis of EGFR using anti- EGFR antibody (AAA125510).EGFR was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-EGFR Antibody (AAA125510) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
EGFR, Polyclonal Antibody (Cat# AAA125510)
Full Name
Anti-EGFR Antibody
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61
Reactivity
Human
Applications
Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of mouse spleen tissues using anti-Caspase-9 p35/Casp9 antibody (AAA125515).Overlay histogram showing mouse spleen tissues stained with AAA125515 (Blue line). The tissues were blocked with 10% normal goat serum. And then incubated with rabbit anti-Caspase-9 p35/Casp9 Antibody (AAA125515, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Caspase-9 p35/Casp9, Polyclonal Antibody (Cat# AAA125515)
Full Name
Anti-Caspase-9 p35/Casp9 Antibody
Gene Names
Casp9; Mch6; APAF-3; CASP-9; AI115399; AW493809; ICE-LAP6; Caspase-9
Reactivity
Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of 293T cells using anti-PINK1 antibody (AAA125528).Overlay histogram showing 293T cells stained with AAA125528 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PINK1 Antibody (AAA125528, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PINK1, Polyclonal Antibody (Cat# AAA125528)
Full Name
Anti-PINK1 Antibody
Gene Names
PINK1; BRPK; PARK6
Reactivity
Human
Applications
Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of human PBMC cells using anti-IL15 antibody (AAA125529).Overlay histogram showing human PBMC cells stained with AAA125529 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IL15 Antibody (AAA125529, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
IL15, Polyclonal Antibody (Cat# AAA125529)
Full Name
Anti-IL15 Antibody
Gene Names
IL15; IL-15
Reactivity
Human, Rat
Applications
Direct ELISA, Flow Cytometry, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
Neurofibromin/NF1, Polyclonal Antibody (Cat# AAA125388)
Full Name
Anti-Neurofibromin/NF1 Antibody
Gene Names
NF1; WSS; NFNS; VRNF
Reactivity
Human
Applications
Direct ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Figure 1. Western blot analysis of AXL using anti-AXL antibody.)
AXL, Polyclonal Antibody (Cat# AAA125395)
Full Name
Anti-AXL Antibody
Gene Names
AXL; ARK; UFO; JTK11; Tyro7
Reactivity
Human
Applications
Direct ELISA, Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
CD1D, Polyclonal Antibody (Cat# AAA125396)
Full Name
Anti-CD1D Antibody
Gene Names
CD1D; R3; CD1A; R3G1
Reactivity
Human, Rat
Applications
Direct ELISA, Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Figure 1. Western blot analysis of FLI1 using anti-FLI1 antibody.)
FLI1, Polyclonal Antibody (Cat# AAA125398)
Full Name
Anti-FLI1 Antibody
Gene Names
FLI1; EWSR2; SIC-1; BDPLT21
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Figure 1. Western blot analysis of Olr1 using anti-Olr1 antibody.)
LOX 1/Olr1, Polyclonal Antibody (Cat# AAA125408)
Full Name
Anti-LOX 1/Olr1 Antibody
Gene Names
Olr1; LOX-1; Oldr1; Oldlr1
Reactivity
Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Figure 1. Western blot analysis of RBPJ using anti-RBPJ antibody.)
RBPJK/RBPJ, Polyclonal Antibody (Cat# AAA125409)
Full Name
Anti-RBPJK/RBPJ Antibody
Gene Names
RBPJ; SUH; csl; AOS3; CBF1; KBF2; CBF-1; RBP-J; RBPJK; IGKJRB; RBP-JK; RBPSUH; IGKJRB1; RBP-J kappa
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Figure 1. Western blot analysis of FGA using anti-FGA antibody.)
Fibrinogen alpha chain/FGA, Polyclonal Antibody (Cat# AAA125410)
Full Name
Anti-Fibrinogen alpha chain/FGA Antibody
Gene Names
FGA; Fib2
Reactivity
Human
Applications
Direct ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Figure 1. Western blot analysis of Tnfrsf8 using anti-Tnfrsf8 antibody.)
CD30/Tnfrsf8, Polyclonal Antibody (Cat# AAA125420)
Full Name
Anti-CD30/Tnfrsf8 Antibody
Gene Names
Tnfrsf8; Ki; Cd30; Ki-1; D1S166E
Reactivity
Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
FHL, Polyclonal Antibody (Cat# AAA125421)
Full Name
Anti-FHL Antibody
Gene Names
FHL1; KYOT; SLIM; FCMSU; FHL-1; FHL1A; FHL1B; FLH1A; SLIM1; XMPMA; RBMX1A; RBMX1B; SLIM-1; SLIMMER
Reactivity
Human
Applications
Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
FHL, Polyclonal Antibody (Cat# AAA125422)
Full Name
Anti-FHL Antibody
Gene Names
Fhl1; KyoT; SLIM; FHL-1; SLIM-1; RAM14-1
Reactivity
Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Figure 1. Western blot analysis of KDM6B using anti-KDM6B antibody.)
KDM6B/JMJD3, Polyclonal Antibody (Cat# AAA125423)
Full Name
Anti-KDM6B/JMJD3 Antibody
Gene Names
KDM6B; JMJD3; NEDCFSA
Reactivity
Human, Mouse
Applications
Direct ELISA, Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Figure 1. Western blot analysis of CARD9 using anti-CARD9 antibody.)
CARD9, Polyclonal Antibody (Cat# AAA125425)
Full Name
Anti-CARD9 Antibody
Gene Names
CARD9; CANDF2; hCARD9
Reactivity
Human
Applications
Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Figure 1. Western blot analysis of Pecam1 using anti-Pecam1 antibody.)
CD31/Pecam1, Polyclonal Antibody (Cat# AAA125430)
Full Name
Anti-CD31/Pecam1 Antibody
Gene Names
Pecam1; CD31; Pecam
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
PHEX, Polyclonal Antibody (Cat# AAA125440)
Full Name
Anti-PHEX Antibody
Gene Names
PHEX; HYP; PEX; XLH; HPDR; HYP1; LXHR; HPDR1
Reactivity
Human, Mouse, Rat, Monkey
Applications
Direct ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
GLUT9/SLC2A9, Polyclonal Antibody (Cat# AAA125447)
Full Name
Anti-GLUT9/SLC2A9 Antibody
Gene Names
SLC2A9; GLUT9; GLUTX; UAQTL2; URATv1
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Figure 1. Western blot analysis of CBR1 using anti-CBR1 antibody.)
CBR1, Polyclonal Antibody (Cat# AAA125455)
Full Name
Anti-CBR1 Antibody
Gene Names
CBR1; CBR; hCBR1; SDR21C1
Reactivity
Human
Applications
Direct ELISA, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Figure 1. Western blot analysis of FOXE1 using anti-FOXE1 antibody.)
FOXE1, Polyclonal Antibody (Cat# AAA125456)
Full Name
Anti-FOXE1 Antibody
Gene Names
FOXE1; TTF2; FOXE2; HFKH4; HFKL5; NMTC4; TITF2; TTF-2; FKHL15
Reactivity
Human
Applications
Direct ELISA, Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Figure 1. Western blot analysis of PPM1A using anti-PPM1A antibody.)
PPM1A, Polyclonal Antibody (Cat# AAA125457)
Full Name
Anti-PPM1A Antibody
Gene Names
PPM1A; PP2CA; PP2Calpha; PP2C-ALPHA
Reactivity
Human
Applications
Direct ELISA, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Figure 1. Western blot analysis of PLSCR1 using anti-PLSCR1 antibody.)
Scramblase 1/PLSCR1, Polyclonal Antibody (Cat# AAA125461)
Full Name
Anti-Scramblase 1/PLSCR1 Antibody
Gene Names
PLSCR1; MMTRA1B
Reactivity
Human, Mouse
Applications
Direct ELISA, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of A549 cells using anti-Endothelin A Receptor/ET-A/ EDNRA antibody (AAA125624).Overlay histogram showing A549 cells stained with AAA125624 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Endothelin A Receptor/ET-A/ EDNRA Antibody (AAA125624,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Endothelin A Receptor/ET-A/EDNRA, Polyclonal Antibody (Cat# AAA125624)
Full Name
Anti-Endothelin A Receptor/ET-A/EDNRA Antibody
Gene Names
EDNRA; ETA; ET-A; ETAR; ETRA; ETA-R; hET-AR
Reactivity
Human
Applications
Direct ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of RAW264. 7 cells using anti-Tigit antibody (AAA125629).Overlay histogram showing RAW264. 7 cells stained with AAA125629 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Tigit Antibody (AAA125629, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Tigit, Polyclonal Antibody (Cat# AAA125629)
Full Name
Anti-Tigit Antibody
Gene Names
Tigit; Vstm3
Reactivity
Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 4. Flow Cytometry analysis of mouse spleen tissue using anti-CD73/Nt5e antibody (AAA125634).Overlay histogram showing mouse spleen tissue stained with AAA125634 (Blue line). The tissue was blocked with 10% normal goat serum. And then incubated with rabbit anti-CD73/Nt5e Antibody (AAA125634, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
CD73/Nt5e, Polyclonal Antibody (Cat# AAA125634)
Full Name
Anti-CD73/Nt5e Antibody
Gene Names
Nt5e; NT; Nt5; eNT; CD73; 5'-NT; AI447961; 2210401F01Rik
Reactivity
Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 5. Flow Cytometry analysis of HL-60 cells using anti-DR1 antibody (AAA125641).Overlay histogram showing HL-60 cells stained with AAA125641(Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DR1 Antibody (AAA125641, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
DR1, Polyclonal Antibody (Cat# AAA125641)
Full Name
Anti-DR1 Antibody
Gene Names
DR1; NC2; NC2-BETA
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
What are Polyclonal Antibodies?
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
- Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
- Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
- ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
- Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
- Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.