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▼Polyclonal Antibodies
At AAA Biotech also known as AAA Bio or AAABio, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
Viewing 2700-2750 of 96812 product results
FCM/FACS (Flow Cytometry)
(Figure 4. Flow Cytometry analysis of PC-3 cells using anti-MAP1S antibody (AAA127226).Overlay histogram showing PC-3 cells stained with AAA127226 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MAP1S Antibody (AAA127226, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
MAP1S, Polyclonal Antibody (Cat# AAA127226)
Full Name
Anti-MAP1S Antibody Picoband
Gene Names
MAP1S; MAP8; BPY2IP1; C19orf5; VCY2IP1; VCY2IP-1
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of HL-60 cells using anti-RASSF2 antibody (AAA127229).Overlay histogram showing HL-60 cells stained with AAA127229 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RASSF2 Antibody (AAA127229, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
RASSF2, Polyclonal Antibody (Cat# AAA127229)
Full Name
Anti-RASSF2 Antibody Picoband
Gene Names
RASSF2; CENP-34; RASFADIN
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Figure 2. IF analysis of GCM1 using anti-GCM1 antibody (AAA127235).GCM1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-GCM1 Antibody (AAA127235) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
GCM1, Polyclonal Antibody (Cat# AAA127235)
Full Name
Anti-GCM1 Antibody Picoband
Gene Names
GCM1; GCMA; hGCMa
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of MCF-7 cells using anti-GWL/MASTL antibody (AAA127238).Overlay histogram showing MCF-7 cells stained with AAA127238 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GWL/MASTL Antibody (AAA127238, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
GWL/MASTL, Polyclonal Antibody (Cat# AAA127238)
Full Name
Anti-GWL/MASTL Antibody Picoband
Gene Names
MASTL; GW; GWL; THC2; hGWL; MAST-L; GREATWALL; RP11-85G18.2
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 4. Flow Cytometry analysis of PC-3 cells using anti-PSE/SPDEF antibody (AAA127243).Overlay histogram showing PC-3 cells stained with AAA127243 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PSE/SPDEF Antibody (AAA127243, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
PSE/SPDEF, Polyclonal Antibody (Cat# AAA127243)
Full Name
Anti-PSE/SPDEF Antibody Picoband
Gene Names
SPDEF; PDEF; bA375E1.3
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of PC-3 cells using anti-HELLS antibody (AAA127248).Overlay histogram showing PC-3 cells stained with AAA127248 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HELLS Antibody (AAA127248, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
HELLS, Polyclonal Antibody (Cat# AAA127248)
Full Name
Anti-HELLS Antibody Picoband
Gene Names
HELLS; LSH; PASG; SMARCA6; Nbla10143
Reactivity
Human, Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of HEL cells using anti-RGS9 antibody (AAA127251).Overlay histogram showing HEL cells stained with AAA127251 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RGS9 Antibody (AAA127251, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
RGS9, Polyclonal Antibody (Cat# AAA127251)
Full Name
Anti-RGS9 Antibody Picoband
Gene Names
RGS9; PERRS; RGS9L
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Figure 2. IF analysis of SMTN using anti-SMTN antibody (AAA127261) and anti-Beta Tubulin antibody (M01857-3).SMTN was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-SMTN Antibody (AAA127261) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and FITC Conjugated Goat Anti-Mouse IgG (BA1101) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
SMTN, Polyclonal Antibody (Cat# AAA127261)
Full Name
Anti-SMTN Antibody Picoband
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IP (Immunoprecipitation)
(Figure 3. Immunoprecipitating OXSR1 in U251 whole cell lysate .Western blot analysis of OXSR1 using anti-OXSR1 antibody (AAA127278).Lane 1: U251 whole cell lysates (30ug)Lane 2: Rabbit control IgG instead of anti-OXSR1 antibody in U251 whole cell lysate.Lane 3: anti-OXSR1 antibody (2ug) + U251 whole cell lysate (500ug)After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-OXSR1 antigen affinity purified polyclonal antibody (AAA127278) at a dilution of 0.5ug/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for OXSR1 at approximately 58 kDa. The expected band size for OXSR1 is at 58 kDa.)
OXSR1, Polyclonal Antibody (Cat# AAA127278)
Full Name
Anti-OXSR1 Antibody Picoband
Gene Names
OXSR1; OSR1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunoprecipitation, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of RT4 cells using anti-C19orf2/URI1 antibody (AAA127280).Overlay histogram showing RT4 cells stained with AAA127280 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-C19orf2/URI1 Antibody (AAA127280, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
C19orf2/URI1, Polyclonal Antibody (Cat# AAA127280)
Full Name
Anti-C19orf2/URI1 Antibody Picoband
Gene Names
URI1; RMP; URI; NNX3; C19orf2; PPP1R19
Reactivity
Human, Mouse
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of HepG2 cells using anti-RRN3 antibody (AAA127298).Overlay histogram showing HepG2 cells stained with AAA127298 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RRN3 Antibody (AAA127298, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
RRN3, Polyclonal Antibody (Cat# AAA127298)
Full Name
Anti-RRN3 Antibody Picoband
Gene Names
RRN3; TIFIA; A-270G1.2
Reactivity
Human
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of HL-60 cells using anti-EIF4ENIF1 antibody (AAA127305).Overlay histogram showing HL-60 cells stained with AAA127305 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EIF4ENIF1 Antibody (AAA127305, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
EIF4ENIF1, Polyclonal Antibody (Cat# AAA127305)
Full Name
Anti-EIF4ENIF1 Antibody Picoband
Gene Names
EIF4ENIF1; 4E-T; Clast4
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of U251 cells using anti-BOK antibody (AAA127311).Overlay histogram showing U251 cells stained with AAA127311 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BOK Antibody (AAA127311, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
BOK, Polyclonal Antibody (Cat# AAA127311)
Full Name
Anti-BOK Antibody Picoband
Gene Names
BOK; BOKL; BCL2L9
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of HCT116 cells using anti-ST13 antibody (AAA127318).Overlay histogram showing HCT116 cells stained with AAA127318 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ST13 Antibody (AAA127318, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
ST13, Polyclonal Antibody (Cat# AAA127318)
Full Name
Anti-ST13 Antibody Picoband
Gene Names
ST13; HIP; HOP; P48; AAG2; SNC6; HSPABP; FAM10A1; FAM10A4; HSPABP1; PRO0786
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 4. Flow Cytometry analysis of JK cells using anti-MAGEL2 antibody (AAA127355).Overlay histogram showing JK cells stained with AAA127355 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MAGEL2 Antibody (AAA127355, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
MAGEL2, Polyclonal Antibody (Cat# AAA127355)
Full Name
Anti-MAGEL2 Antibody Picoband
Gene Names
MAGEL2; PWLS; nM15; NDNL1
Reactivity
Human
Applications
Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of 293T cells using anti-XPA antibody (AAA127042).Overlay histogram showing 293T cells stained with AAA127042 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-XPA Antibody (AAA127042, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
XPA, Polyclonal Antibody (Cat# AAA127042)
Full Name
Anti-XPA Antibody Picoband
Gene Names
XPA; XP1; XPAC
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of RT4 cells using anti-NUMB antibody (AAA127043).Overlay histogram showing RT4 cells stained with AAA127043 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NUMB Antibody (AAA127043, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
NUMB, Polyclonal Antibody (Cat# AAA127043)
Full Name
Anti-NUMB Antibody Picoband
Gene Names
NUMB; S171; C14orf41; c14_5527
Reactivity
Human, Monkey, Mouse, Rat
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 5. Flow Cytometry analysis of MCF-7 cells using anti-Chromogranin A/CHGA antibody (AAA127045).Overlay histogram showing MCF-7 cells stained with AAA127045 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Chromogranin A/CHGA Antibody (AAA127045, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
Chromogranin A/CHGA, Polyclonal Antibody (Cat# AAA127045)
Full Name
Anti-Chromogranin A/CHGA Antibody Picoband
Gene Names
CHGA; CGA
Reactivity
Human
Applications
Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of 293T cells using anti-PDK1 antibody (AAA127048).Overlay histogram showing 293T cells stained with AAA127048 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PDK1 Antibody (AAA127048, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PDK1, Polyclonal Antibody (Cat# AAA127048)
Full Name
Anti-PDK1 Antibody Picoband
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of U937 cells using anti-PRMT1 antibody (AAA127059).Overlay histogram showing U937 cells stained with AAA127059 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRMT1 Antibody (AAA127059, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PRMT1, Polyclonal Antibody (Cat# AAA127059)
Full Name
Anti-PRMT1 Antibody Picoband
Gene Names
PRMT1; ANM1; HCP1; IR1B4; HRMT1L2
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of HepG2 cells using anti-Spermidine synthase/SRM antibody (AAA127068).Overlay histogram showing HepG2 cells stained with AAA127068 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Spermidine synthase/SRM Antibody (AAA127068, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
Spermidine synthase/SRM, Polyclonal Antibody (Cat# AAA127068)
Full Name
Anti-Spermidine synthase/SRM Antibody Picoband
Gene Names
SRM; PAPT; SPS1; SPDSY; SRML1
Reactivity
Human
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of MCF-7 cells using anti-MVD antibody (AAA127073).Overlay histogram showing MCF-7 cells stained with AAA127073 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MVD Antibody (AAA127073, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
MVD, Polyclonal Antibody (Cat# AAA127073)
Full Name
Anti-MVD Antibody Picoband
Gene Names
MVD; MPD; MDDase; FP17780
Reactivity
Human, Mouse
Applications
Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Figure 3. IF analysis of Cytokeratin 1/Krt1 using anti-Cytokeratin 1/Krt1 antibody (AAA127075).Cytokeratin 1/Krt1 was detected in a paraffin-embedded section of mouse skin tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5ug/mL rabbit anti-Cytokeratin 1/Krt1 Antibody (AAA127075) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
Cytokeratin 1/Krt1, Polyclonal Antibody (Cat# AAA127075)
Full Name
Anti-Cytokeratin 1/Krt1 Antibody Picoband
Gene Names
Krt1; Krt86; Krt2-1; Krt-2.1
Reactivity
Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of 293T cells using anti-NCOA2 antibody (AAA127079).Overlay histogram showing 293T cells stained with AAA127079 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NCOA2 Antibody (AAA127079, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
NCOA2, Polyclonal Antibody (Cat# AAA127079)
Full Name
Anti-NCOA2 Antibody Picoband
Gene Names
NCOA2; SRC2; TIF2; GRIP1; KAT13C; NCoA-2; bHLHe75
Reactivity
Human
Applications
Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of JK cells using anti-RING1 antibody (AAA127083).Overlay histogram showing JK cells stained with AAA127083 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RING1 Antibody (AAA127083, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
RING1, Polyclonal Antibody (Cat# AAA127083)
Full Name
Anti-RING1 Antibody Picoband
Gene Names
RING1; RNF1; RING1A
Reactivity
Human, Rat
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of Raji cells using anti-ROR2 antibody (AAA127084).Overlay histogram showing Raji cells stained with AAA127084 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-ROR2 Antibody (AAA127084, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
ROR2, Polyclonal Antibody (Cat# AAA127084)
Full Name
Anti-ROR2 Antibody Picoband
Gene Names
ROR2; BDB; BDB1; NTRKR2
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 5. Flow Cytometry analysis of HEL cells using anti-HTRA2 antibody (AAA127088).Overlay histogram showing HEL cells stained with AAA127088 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HTRA2 Antibody (AAA127088, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
HTRA2, Polyclonal Antibody (Cat# AAA127088)
Full Name
Anti-HTRA2 Antibody Picoband
Gene Names
HTRA2; OMI; PARK13; PRSS25
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of HEL cells using anti-Melan-A/MLANA antibody (AAA127095).Overlay histogram showing HEL cells stained with AAA127095 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Melan-A/MLANA Antibody (AAA127095, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Melan-A/MLANA, Polyclonal Antibody (Cat# AAA127095)
Full Name
Anti-Melan-A/MLANA Antibody Picoband
Gene Names
MLANA; MART1; MART-1
Reactivity
Human, Mouse
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of RT4 cells using anti-CRKL antibody (AAA127100).Overlay histogram showing RT4 cells stained with AAA127100 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CRKL Antibody (AAA127100, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
CRKL, Polyclonal Antibody (Cat# AAA127100)
Full Name
Anti-CRKL Antibody Picoband
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of U251 cells using anti-PUM1 antibody (AAA127102).Overlay histogram showing U251 cells stained with AAA127102 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PUM1 Antibody (AAA127102, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
PUM1, Polyclonal Antibody (Cat# AAA127102)
Full Name
Anti-PUM1 Antibody Picoband
Gene Names
PUM1; PUMH; HSPUM; PUMH1; PUML1; SCA47
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 5. Flow Cytometry analysis of Hela cells using anti-PDK4 antibody (AAA127103).Overlay histogram showing Hela cells stained with AAA127103 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PDK4 Antibody (AAA127103, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PDK4, Polyclonal Antibody (Cat# AAA127103)
Full Name
Anti-PDK4 Antibody Picoband
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of 293T cells using anti-RPS19 antibody (AAA127107).Overlay histogram showing 293T cells stained with AAA127107 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPS19 Antibody (AAA127107, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
RPS19, Polyclonal Antibody (Cat# AAA127107)
Full Name
Anti-RPS19 Antibody Picoband
Gene Names
RPS19; DBA; S19; DBA1
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of Hela cells using anti-hnRNP E2/PCBP2 antibody (AAA127115).Overlay histogram showing Hela cells stained with AAA127115 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-hnRNP E2/PCBP2 Antibody (AAA127115, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
hnRNP E2/PCBP2, Polyclonal Antibody (Cat# AAA127115)
Full Name
Anti-hnRNP E2/PCBP2 Antibody Picoband
Gene Names
PCBP2; HNRPE2; HNRNPE2; hnRNP-E2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of 293T cells using anti-WDR33 antibody (AAA127596).Overlay histogram showing 293T cells stained with AAA127596 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-WDR33 Antibody (AAA127596, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
WDR33, Polyclonal Antibody (Cat# AAA127596)
Full Name
Anti-WDR33 Antibody Picoband
Gene Names
WDR33; NET14; WDC146
Reactivity
Human
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of HEL cells using anti-PDXP antibody (AAA127600).Overlay histogram showing HEL cells stained with AAA127600 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PDXP Antibody (AAA127600, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PDXP, Polyclonal Antibody (Cat# AAA127600)
Full Name
Anti-PDXP Antibody Picoband
Gene Names
PDXP; CIN; PLP; dJ37E16.5
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohiostchemistry)
(Figure 2. IHC analysis of LY6D using anti-LY6D antibody (AAA127604).LY6D was detected in a paraffin-embedded section of human skin cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LY6D Antibody (AAA127604) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)
LY6D, Polyclonal Antibody (Cat# AAA127604)
Full Name
Anti-LY6D Antibody Picoband
Gene Names
LY6D; E48; Ly-6D
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of Hela cells using anti-RRP8 antibody (AAA127621).Overlay histogram showing Hela cells stained with AAA127621 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RRP8 Antibody (AAA127621, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
RRP8, Polyclonal Antibody (Cat# AAA127621)
Full Name
Anti-RRP8 Antibody Picoband
Gene Names
RRP8; KIAA0409
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Figure 2. IF analysis of LYAR using anti-LYAR antibody (AAA127622) and anti-Beta Tubulin antibody (M01857-3).LYAR was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-LYAR Antibody (AAA127622) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Rabbit IgG and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
LYAR, Polyclonal Antibody (Cat# AAA127622)
Full Name
Anti-LYAR Antibody Picoband
Gene Names
LYAR; ZLYAR; ZC2HC2
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Figure 2. IF analysis of HSD3B7 using anti-HSD3B7 antibody (AAA127624).HSD3B7 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-HSD3B7 Antibody (AAA127624) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
HSD3B7, Polyclonal Antibody (Cat# AAA127624)
Full Name
Anti-HSD3B7 Antibody Picoband
Gene Names
HSD3B7; CBAS1; PFIC4; SDR11E3
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of HepG2 cells using anti-C19orf61/SMG9 antibody (AAA127639).Overlay histogram showing HepG2 cells stained with AAA127639 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-C19orf61/SMG9 Antibody (AAA127639, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
C19orf61/SMG9, Polyclonal Antibody (Cat# AAA127639)
Full Name
Anti-C19orf61/SMG9 Antibody Picoband
Gene Names
SMG9; C19orf61; F17127_1
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of U87 cells using anti-NDUFA8 antibody (AAA127661).Overlay histogram showing U87 cells stained with AAA127661 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NDUFA8 Antibody (AAA127661, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
NDUFA8, Polyclonal Antibody (Cat# AAA127661)
Full Name
Anti-NDUFA8 Antibody Picoband
Gene Names
NDUFA8; PGIV; CI-19KD; CI-PGIV
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Figure 4. IF analysis of NUDCD1 using anti-NUDCD1 antibody (AAA127674) and anti-Beta Tubulin antibody (M01857-3).NUDCD1 was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-NUDCD1 Antibody (AAA127674) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
NUDCD1, Polyclonal Antibody (Cat# AAA127674)
Full Name
Anti-NUDCD1 Antibody Picoband
Gene Names
NUDCD1; CML66; OVA66
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 5. Flow Cytometry analysis of U251 cells using anti-SCNM1 antibody (AAA127689).Overlay histogram showing U251 cells stained with AAA127689 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SCNM1 Antibody (AAA127689, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
SCNM1, Polyclonal Antibody (Cat# AAA127689)
Full Name
Anti-SCNM1 Antibody Picoband
Gene Names
SCNM1; MGC3180
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of A549 cells using anti-PRDM11 antibody (AAA127696).Overlay histogram showing A549 cells stained with AAA127696 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRDM11 Antibody (AAA127696, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
PRDM11, Polyclonal Antibody (Cat# AAA127696)
Full Name
Anti-PRDM11 Antibody Picoband
Gene Names
PRDM11; PFM8
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 5. Flow Cytometry analysis of SH-SY5Y cells using anti-RGL3 antibody (AAA127734).Overlay histogram showing SH-SY5Y cells stained with AAA127734 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RGL3 Antibody (AAA127734, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
RGL3, Polyclonal Antibody (Cat# AAA127734)
Full Name
Anti-RGL3 Antibody Picoband
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Figure 2. IF analysis of PRTFDC1 using anti-PRTFDC1 antibody (AAA127736).PRTFDC1 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-PRTFDC1 Antibody (AAA127736) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
PRTFDC1, Polyclonal Antibody (Cat# AAA127736)
Full Name
Anti-PRTFDC1 Antibody Picoband
Gene Names
PRTFDC1; HHGP
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Figure 2. IF analysis of PBX4 using anti-PBX4 antibody (AAA127742) and anti-Beta Tubulin antibody (M01857-3).PBX4 was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-PBX4 Antibody (AAA127742) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and FITC Conjugated Goat Anti-Mouse IgG (BA1101) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
PBX4, Polyclonal Antibody (Cat# AAA127742)
Full Name
Anti-PBX4 Antibody Picoband
Reactivity
Human, Mouse
Applications
Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 5. Flow Cytometry analysis of Jurkat cells using anti-RBM25 antibody (AAA127449).Overlay histogram showing Jurkat cells stained with AAA127449 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RBM25 Antibody (AAA127449, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
RBM25, Polyclonal Antibody (Cat# AAA127449)
Full Name
Anti-RBM25 Antibody Picoband
Gene Names
RBM25; S164; NET52; RNPC7; Snu71; RED120; fSAP94; MGC105088; MGC117168
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of SH-SY5Y cells using anti-SYNPO2 antibody (AAA127462).Overlay histogram showing SH-SY5Y cells stained with AAA127462 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SYNPO2 Antibody (AAA127462, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
SYNPO2, Polyclonal Antibody (Cat# AAA127462)
Full Name
Anti-SYNPO2 Antibody Picoband
Reactivity
Human
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of HepG2 cells using anti-MYT1L antibody (AAA127482).Overlay histogram showing HepG2 cells stained with AAA127482 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MYT1L Antibody (AAA127482, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
MYT1L, Polyclonal Antibody (Cat# AAA127482)
Full Name
Anti-MYT1L Antibody Picoband
Gene Names
MYT1L; NZF1; ZC2HC4B
Reactivity
Human
Applications
Flow Cytometry, Western Blot, Immunofluorescence, Immunocytochemistry
Purity
Immunogen affinity purified.
Pricing
What are Polyclonal Antibodies?
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
- Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
- Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
- ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
- Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
- Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.