At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
FCM/FACS (Flow Cytometry) (Figure 4. Flow Cytometry analysis of U251 cells using anti-GGCX antibody (AAA126239).Overlay histogram showing U251 cells stained with AAA126239 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GGCX Antibody (AAA126239, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
IF (Immunofluorescence) (Figure 3. IF analysis of GGCX using anti-GGCX antibody (AAA126239).GGCX was detected in an immunocytochemical section of CACO-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL rabbit anti-GGCX Antibody (AAA126239) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
IHC (Immunohiostchemistry) (Figure 2. IHC analysis of GGCX using anti-GGCX antibody (AAA126239).GGCX was detected in a paraffin-embedded section of human hepatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-GGCX Antibody (AAA126239) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
WB (Western Blot) (Figure 1. Western blot analysis of GGCX using anti-GGCX antibody (AAA126239).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human MCF-7 whole cell lysates,Lane 2: human HEK293 whole cell lysates,Lane 3: human HepG2 whole cell lysates,Lane 4: human PC-3 whole cell lysates,Lane 5: mouse HEPA1-6 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GGCX antigen affinity purified polyclonal antibody (#AAA126239) at 0.25 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GGCX at approximately 88 kDa. The expected band size for GGCX is at 88 kDa.)
IHC (Immunohiostchemistry) ((3.8ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
WB (Western Blot) ((2ug/ml) staining of lysates of cell line HepG2 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
WB (Western Blot) ((0.1ug/ml) staining of Mouse (A) and Rat (B) Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
WB (Western Blot) ((1ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
WB (Western Blot) (Figure 2 Western Blot Validation with SARS-CoV-2 (COVID-19) Membrane ProteinLoading: 30 ng per lane of SARS-CoV-2 (COVID-19) membrane protein (10-429).Antibodies: SARS-CoV-2 (COVID-19) membrane, 9157, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: 0.25 μg/mL andLane 2: 0.5 μg/mL)
ELISA (Figure 1 ELISA Validation with SARS-CoV-2 (COVID-19) Membrane ProteinAntibodies: SARS-CoV-2 (COVID-19) Membrane Antibody, 9157. A direct ELISA was performed using SARS-CoV-2 membrane recombinant protein (10-429) as coating antigen and the anti-SARS-CoV-2 (COVID-19) Membrane antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/ml)
SARS-CoV-2 (COVID-19) Membrane Antibody is affinity chromatography
Pricing
WB (Western Blot) (Figure. Western blot analysis of IFN gamma using anti-IFN gamma antibody (AAA44940).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane : Recombinant Rat IFN gamma Protein 0.5ngAfter Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFN gamma antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IFN gamma at approximately 22KD. The expected band size for IFN gamma is at 22KD.)
IP (Immunoprecipitation) (GPT2 was immunoprecipitated using:Lane A:0.5 mg U251MG Whole Cell Lysate4 uL anti-GPT2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-GPT2 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 58 kDaObserved band size :55 kDa)
IHC (Immunohistochemisry) (Immunochemical staining of human GPT2 in human stomach with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry) (Immunochemical staining of human GPT2 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
WB (Western Blot) (Anti-GPT2 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: U251MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:58 kDa)
IHC (Immunohiostchemistry) (Immunohistochemistry of Rkhd4 in human brain tissue with Rkhd4 antibody at 2.5 ug/mL.)
WB (Western Blot) (Western blot analysis of Rkhd4 in SK-N-SH cell lysate with Rkhd4 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Her3/ErbB3(Ab-1328) Antibody (left) or the same antibody preincubated with blocking peptide(right).)
WB (Western Blot) (Western blot analysis of extracts from A431 cells using Her3/ErbB3(Ab-1328) Antibody.)
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using at dilution 1/20.)
Application Data (Gel: 10%SDS-PAGE Lysates (from left to right): Mouse pancreas tissue, Hela cell and mouse liver tissue, A549 and 293T cell Amount of lysate: 40ug per lane Primary antibody: 1/500 dilution Secondary antibody dilution: 1/8000 Exposure time: 20 seconds)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded rat hippocampal region tissue from a model with Alzheimer)
WB (Western Blot) (Western blot analysis of extracts from mouse brain tissue using Tau(Phospho-Thr231) Antibody and the same antibody preincubated with blocking peptide.)
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin
IHC (Immunohistochemisry) (Formalin-fixed and paraffin-embedded human breast carcinoma reacted with PI3KCA Antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot) (Western blot analysis of anti-PI3KCA Antibody (Center) Pab in 293 cell line lysates transiently transfected with the PIK3CA gene (1ug/lane). PI3KCA (arrow) was detected using the purified Pab.)
WB (Western Blot) (Western blot analysis of anti-PIK3CA Antibody (Center) Pab in K562 cell line lysates (35ug/lane). PIK3CA (arrow) was detected using the purified Pab)
IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human testis tissue reacted with M6PR antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot) (Western blot analysis of M6PR Antibody (N-term) in mouse brain tissue lysates (35ug/lane). M6PR (arrow) was detected using the purified Pab.)
IHC (Immunohistochemisry) (Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with MMP13 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
WB (Western Blot) (Western blot analysis of MMP13(arrow) using rabbit polyclonal MMP13 Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MMP13 gene (Lane 2) (Origene Technologies).)
IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with CK1g2 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot) (Western blot analysis of anti-CK1g2 C-term Pab in mouse muscle tissue lysate. CK1g2 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
IHC (Immunohiostchemistry) (DPP10 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DPP10 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot) (Western blot analysis of DPP10 Antibody (Center) in Y79, MDA-MB231 cell line and mouse liver, brain tissue lysates (35ug/lane). DPP10 (arrow) was detected using the purified Pab.)
IHC (Immunohiostchemistry) (HRC Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HRC Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot) (HRC Antibody (Center) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the HRC antibody detected the HRC protein (arrow).)
FCM/FACS (Flow Cytometry) (ASM3A Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry) (ASM3A Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ASM3A Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot) (Western blot analysis of ASM3A Antibody (C-term) in MDA-MB435 cell line lysates (35ug/lane). ASM3A (arrow) was detected using the purified Pab.)
FCM/FACS (Flow Cytometry) (DSC1 Antibody (C-term) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with DSC1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot) (Western blot analysis of DSC1 Antibody (C-term) in A375 cell line lysates (35ug/lane). DSC1 (arrow) was detected using the purified Pab.)
WB (Western Blot) (Western blot analysis of PMCH (arrow) using rabbit polyclonal PMCH Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PMCH gene.)
FCM/FACS (Flow Cytometry) (PMCH Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohistochemisry) (PMCH Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PMCH Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot) (PMCH Antibody (Center) western blot analysis in mouse brain tissue lysates (15ug/lane).This demonstrates the PMCH antibody detected the PMCH protein (arrow).)
WB (Western Blot) (PMCH Antibody (Center) western blot analysis in K562 cell line lysates (15ug/lane).This demonstrates the PMCH antibody detected the PMCH protein (arrow).)
IHC (Immunohiostchemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using ETFB Antibody at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200))
SDS-PAGE (Gel: 12%SDS-PAGE Lysate: 40 ug, Lane 1-6: HEPG2 and Hela cell lysates£¬Human heart tissue£¬mouse brain tissue£¬mouse skeletal muscle tissue£¬human liver tissue lysates, Primary antibody:ETFB antibody at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using TNFRSF11A antibody at 1:3000 dilution.)
IF (Immunofluorescence) (Immunofluorescence analysis of L929 cells using TNFRSF11A Polyclonal Antibody at dilution of 1:100 . Blue: DAPI for nuclear staining.)
IHC (Immunohistochemisry) (p53 Antibody (T55) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of p53 Antibody (T55) for immunohistochemistry. Clinical relevance has not been evaluated.)
IF (Immunofluorescence) (Confocal immunofluorescent analysis of p53 Antibody (T55) with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
WB (Western Blot) (Western blot analysis of TP53(arrow) using rabbit polyclonal p53 Antibdoy (T55). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the TP53 gene (Lane 2) (Origene Technologies).)
WB (Western Blot) (WB Suggested Anti-FUCA1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: HT1080 cell lysateFUCA1 is supported by BioGPS gene expression data to be expressed in HT1080)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using SLC39A3 Polyclonal Antibody at dilution 1:30)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human liver cancer tissue using SLC39A3 Polyclonal Antibody at dilution 1:30)
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.
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