Polyclonal Antibodies

At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.

Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.

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WB (Western Blot) (Western blot analysis of extracts from NIH/3T3 cells, using RGS5 antibody.)

IF (Immunofluorescence) (AAA325068 staining NIH-3T3 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

IHC (Immunohistochemistry) (AAA325068 at 1/100 staining Human prostate tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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RGS5, Polyclonal Antibody (Cat# AAA325068)

Full Name

RGS5 Antibody

Gene Names

RGS5; MST092; MST106; MST129; MSTP032; MSTP092; MSTP106; MSTP129

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Peptide affinity purification

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of extracts from HepG2 cells, using RPL7L1 antibody.)

IF (Immunofluorescence) (AAA325090 staining HepG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

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RPL7L1, Polyclonal Antibody (Cat# AAA325090)

Full Name

RPL7L1 Antibody

Gene Names

RPL7L1; dJ475N16.4

Reactivity

Human

Applications

Immunocytochemistry, Immunofluorescence, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of extracts from HepG2 using IL18BP antibody.)

IF (Immunofluorescence) (AAA325345 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts from rat spleen, using IL18BP Antibody.)

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IL18BP, Polyclonal Antibody (Cat# AAA325345)

Full Name

IL18BP Antibody

Gene Names

IL18BP; IL18BPa

Reactivity

Human, Rat

Applications

Immunocytochemistry, Immunofluorescence, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

IHC (Immunohistochemistry) (AAA321433 at 1/100 staining rat gastric tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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Survivin, Polyclonal Antibody (Cat# AAA321433)

Full Name

Phospho-Survivin (Thr117) Antibody

Gene Names

BIRC5; API4; EPR-1

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The Ab is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of Phospho-Abl (Tyr412) expression in various lysates)

IF (Immunofluorescence) (AAA321446 staining COS7 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of Abl phosphorylation expression in Adriamycin treated COS7 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

IHC (Immunohistochemistry) (AAA321446 at 1/200 staining human brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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Abl, Polyclonal Antibody (Cat# AAA321446)

Full Name

Phospho-Abl (Tyr412) Antibody

Gene Names

ABL1; ABL; JTK7; p150; c-ABL; v-abl; CHDSKM; c-ABL1; bcr/abl

Reactivity

Human, Mouse, Monkey

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of Estrogen Receptor-alpha phosphorylation expression in 293 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

IHC (Immunohistochemisry) (AAA321457 at 1/100 staining human Breast carcinoma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IF (Immunofluorescence) (AAA321457 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of Phospho-Estrogen Receptor alpha (Tyr537) Antibody expression in 293 cells lysates.The lane on the right is treated with the antigen-specific peptide.)

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Estrogen Receptor alpha, Polyclonal Antibody (Cat# AAA321457)

Full Name

Phospho-Estrogen Receptor alpha (Tyr537) Antibody

Gene Names

ESR1; ER; ESR; Era; ESRA; ESTRR; NR3A1

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of Phospho-C-RAF (Ser621) expression in various lysates)

WB (Western Blot) (Western blot analysis of C-RAF phosphorylation expression in UV treated HeLa whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

IHC (Immunohistochemisry) (AAA321461 at 1/200 staining human lymph node tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IF (Immunofluorescence) (AAA321461 staining NIH/3T3 cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)

IF (Immunofluorescence) (AAA321461 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

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C-RAF, Polyclonal Antibody (Cat# AAA321461)

Full Name

Phospho-C-RAF (Ser621) Antibody

Gene Names

RAF1; NS5; CRAF; Raf-1; c-Raf; CMD1NN

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of PKC zeta phosphorylation expression in PMA treated NIH-3T3 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

WB (Western Blot) (Western blot analysis of PKC zeta phosphorylation expression in mouse liver lysates, The lane on the right is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA321697 staining C6 cells treated with lambda phosphatase by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)

IF (Immunofluorescence) (AAA321697 staining NIH-3T3 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

IHC (Immunohistochemistry) (AAA321697 at 1/100 staining human brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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PKC zeta, Polyclonal Antibody (Cat# AAA321697)

Full Name

Phospho-PKC zeta (Thr410) Antibody

Gene Names

PRKCZ; PKC2; PKC-ZETA

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IF (Immunofluorescence) (AAA321276 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

IHC (Immunohistochemisry) (AAA321276 at 1/100 staining human lung carcinoma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IF (Immunofluorescence) (AAA321276 staining 293 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

WB (Western Blot) (Western blot analysis on 293 cell lysate using Phospho-Akt(Ser473) Antibody. The lane on the left is treated with the antigen-specific peptide.)

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Akt, Polyclonal Antibody (Cat# AAA321276)

Full Name

Phospho-Akt(Ser473) Antibody

Gene Names

AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohistochemistry) (Colon tissue HIF1A antibody used at 1/200 on formalin-fixed paraffin embedded tissue.)

WB (Western Blot) (Western blot analysis of extracts of various sample, using hif1a antibody.)

IF (Immunofluorescence) (AAA321279 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

WB (Western Blot) (Western blot analysis on LOVO using HIF1a antibody, The lane on the left is blocked with the antigen-specific peptide.)

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HIF1A, Polyclonal Antibody (Cat# AAA321279)

Full Name

HIF1A Antibody

Gene Names

HIF1A; HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA; HIF-1-alpha

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of extracts of C2C12 cells treated with TPA, using Phospho-CX43(Tyr265)Antibody.)

IHC (Immunohistochemistry) (AAA321290 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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CX43, Polyclonal Antibody (Cat# AAA321290)

Full Name

Phospho-CX43(Tyr265) Antibody

Gene Names

GJA1; HSS; CMDR; CX43; EKVP; GJAL; ODDD; AVSD3; EKVP3; HLHS1; PPKCA

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IF (Immunofluorescence) (AAA321135 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis on Jurkat cell lysate using AKAP14 Antibody, The lane on the left is treated with the antigen-specific peptide.)

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AKAP14, Polyclonal Antibody (Cat# AAA321135)

Full Name

AKAP14 Antibody

Gene Names

AKAP14; AKAP28; PRKA14

Reactivity

Human

Applications

Immunocytochemistry, Immunofluorescence, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of Phospho-PKC alpha (Thr638) expression in various lysates)

IHC (Immunohistochemisry) (AAA321544 at 1/200 staining human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IF (Immunofluorescence) (AAA321544 staining NIH-3T3 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of PKC alpha phosphorylation expression in UV treated NIH-3T3 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

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PKC alpha, Polyclonal Antibody (Cat# AAA321544)

Full Name

Phospho-PKC alpha (Thr638) Antibody

Gene Names

PRKCA; AAG6; PKCA; PRKACA; PKCI+/-; PKCalpha; PKC-alpha

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of extracts of MOLT-15 cells treated with phosphatase, using Phospho-LCP1 (Tyr28) Antibody.)

IHC (Immunohistochemistry) (AAA321344 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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LCP1, Polyclonal Antibody (Cat# AAA321344)

Full Name

Phospho-LCP1 (Tyr28) Antibody

Gene Names

LCP1; LPL; CP64; PLS2; LC64P; HEL-S-37; L-PLASTIN

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis on NIH-3T3 cell lysate using ERD23 Antibody)

IF (Immunofluorescence) (AAA321224 staining NIH-3T3 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

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ERD23, Polyclonal Antibody (Cat# AAA321224)

Full Name

ERD23 Antibody

Gene Names

KDELR3; ERD2L3

Reactivity

Human, Mouse

Applications

Immunocytochemistry, Immunofluorescence, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

WB (Western Blot) (Western blot analysis of extracts from Mouse testis, using Cytokeratin 5 Ab at 1/1000 dilution.Observed bands:65kD.)

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Keratin 5, Polyclonal Antibody (Cat# AAA320839)

Full Name

Keratin 5 Antibody

Gene Names

KRT5; K5; CK5; DDD; DDD1; EBS2; KRT5A

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IF (Immunofluorescence) (AAA320899 staining HT29 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis on HT29 cell lysate using Gamma-synuclein Antibody, The lane on the left is treated with the antigen-specific peptide.)

IHC (Immunohistochemistry) (AAA320899 at 1/100 staining human breast carcinoma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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Gamma-synuclein, Polyclonal Antibody (Cat# AAA320899)

Full Name

Gamma-synuclein Antibody

Gene Names

SNCG; SR; BCSG1

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of extracts from various samples, using NYREN18 Antibody. Lane 1: 293 treated with blocking peptide. Lane 2: 293; Lane 3: Hepg2;)

IF (Immunofluorescence) (AAA320913 staining COS7 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis on COS7 cell lysate using NYREN18 Antibody, The lane on the left is treated with the antigen-specific peptide.)

IHC (Immunohistochemistry) (AAA320913 at 1/100 staining human liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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NYREN18, Polyclonal Antibody (Cat# AAA320913)

Full Name

NYREN18 Antibody

Gene Names

NUB1; BS4; NUB1L; NYREN18

Reactivity

Human, Mouse, Monkey

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of extracts from 293 and Hela, using PIAS2 Antibody.)

IF (Immunofluorescence) (AAA320914 staining COS7 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts from 293, using PIAS2 Antibody.)

WB (Western Blot) (Western blot analysis on COS7 cell lysate using PIAS2 Antibody, The lane on the left is treated with the antigen-specific peptide.)

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PIAS2, Polyclonal Antibody (Cat# AAA320914)

Full Name

PIAS2 Antibody

Gene Names

PIAS2; DIP; MIZ1; SIZ2; ARIP3; PIASX; ZMIZ4

Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunocytochemistry, Immunofluorescence, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohiostchemistry) (AAA320760 at 1/100 staining human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis on HT29 cell lysate using Gab2 Antibody, The lane on the left is treated with the antigen-specific peptide.)

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GAB2, Polyclonal Antibody (Cat# AAA320760)

Full Name

GAB2 Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of PDCD4 phosphorylation expression in serum treated 293 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

IHC (Immunohistochemisry) (Phospho-PDCD4 (Ser457) Antibody for IHC in human colon tissue)

WB (Western Blot) (Western blot analysis of PDCD4 phosphorylation expression in serum treated Hela whole cell lysates, The lane on the right is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA321753 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

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PDCD4, Polyclonal Antibody (Cat# AAA321753)

Full Name

Phospho-PDCD4 (Ser457) Antibody

Gene Names

PDCD4; H731

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IF (Immunofluorescence) (AAA321776 staining MKN-45 cells treated with Forskolin 30 muM, 20 min by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)

IHC (Immunohistochemistry) (AAA321776 at 1/100 staining human colon cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of DARPP-32 phosphorylation expression in PMA treated NIH-3T3 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

WB (Western Blot) (Western blot analysis of Phospho-DARPP-32 (Thr34) expression in various lysates)

IF (Immunofluorescence) (AAA321776 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

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DARPP-32, Polyclonal Antibody (Cat# AAA321776)

Full Name

Phospho-DARPP-32 (Thr34) Antibody

Gene Names

PPP1R1B; DARPP32; DARPP-32

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IF (Immunofluorescence) (AAA322174 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

IF (Immunofluorescence) (AAA322174 staining HepG2  cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

IHC (Immunohistochemisry) (AAA322174 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IF (Immunofluorescence) (AAA322174 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

WB (Western Blot) (Western blot analysis of Amyloid beta A4 expression in HeLa whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

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Amyloid beta A4, Polyclonal Antibody (Cat# AAA322174)

Full Name

Amyloid beta A4 Antibody

Gene Names

APP; AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; preA4; CTFgamma

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IF (Immunofluorescence) (AAA322190 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of CDC2 expression in HepG2 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

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CDC2, Polyclonal Antibody (Cat# AAA322190)

Full Name

CDC2 Antibody

Gene Names

CDK1; CDC2; CDC28A; P34CDC2

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of eIF4E expression in FBS treated NIH-3T3 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

IHC (Immunohiostchemistry) (AAA322192 at 1/100 staining Human prostate tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IF (Immunofluorescence) (AAA322192 staining NIH-3T3 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

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EIF4E, Polyclonal Antibody (Cat# AAA322192)

Full Name

EIF4E Antibody

Gene Names

EIF4E; CBP; EIF4F; AUTS19; EIF4E1; eIF-4E; EIF4EL1

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohiostchemistry) (AAA322231 at 1/100 staining Mouse colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of ATF2 expression in MDA-MB-435 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

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ATF2, Polyclonal Antibody (Cat# AAA322231)

Full Name

ATF2 Antibody

Gene Names

ATF2; HB16; CREB2; TREB7; CREB-2; CRE-BP1

Reactivity

Human, Mouse, Rat

Applications

Immunoprecipitation, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohistochemisry) (AAA322267 at 1/100 staining Mouse intestine tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IF (Immunofluorescence) (AAA322267 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

WB (Western Blot) (Western blot analysis of CBL expression in Na2VO3 treated HepG2 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

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CBL, Polyclonal Antibody (Cat# AAA322267)

Full Name

CBL Antibody

Gene Names

CBL; CBL2; NSLL; C-CBL; RNF55; FRA11B

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohiostchemistry) (AAA321861 at 1/100 staining human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of BMP7 expression in Jurkat cells)

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BMP7, Polyclonal Antibody (Cat# AAA321861)

Full Name

BMP7 Antibody

Gene Names

BMP7; OP-1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of PKM2 expression in HeLa cell lysate, The lane on the left is treated with the antigen-specific peptide.)

IHC (Immunohistochemistry) (AAA321895 at 1/100 staining human testis tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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PKM2, Polyclonal Antibody (Cat# AAA321895)

Full Name

PKM2 Antibody

Gene Names

PKM; PK3; TCB; OIP3; PKM2; CTHBP; THBP1; HEL-S-30

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IF (Immunofluorescence) (AAA322100 staining A549 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

IHC (Immunohistochemisry) (AAA322100 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IF (Immunofluorescence) (AAA322100 staining MCF-7 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

WB (Western Blot) (Western blot analysis of COX IV expression in Jurkat cells.The lane on the left is treated with the antigen-specific peptide.)

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COX IV, Polyclonal Antibody (Cat# AAA322100)

Full Name

COX IV Antibody

Gene Names

COX4I1; COX4; COXIV; COX4-1; COXIV-1; COX IV-1

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IF (Immunofluorescence) (AAA322134 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of Aurora Kinase expression in serum treated 293 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

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Aurora Kinase, Polyclonal Antibody (Cat# AAA322134)

Full Name

Aurora Kinase Antibody

Gene Names

AURKA; AIK; ARK1; AURA; BTAK; STK6; STK7; STK15; PPP1R47

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohistochemisry) (AAA321982 at 1/100 staining Rat colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IF (Immunofluorescence) (AAA321982 staining HepG2 by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab (AAA321982 1:200) and mouse anti-beta tubulin Ab ( 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L)Ab(Green) were used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of MMP 2 expression in LOVO cells.The lane on the left is treated with the antigen-specific peptide.)

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MMP 2, Polyclonal Antibody (Cat# AAA321982)

Full Name

MMP 2 Antibody

Gene Names

MMP2; CLG4; MONA; CLG4A; MMP-2; TBE-1; MMP-II

Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Western Blot

Purity

Peptide affinity purification

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded human epityphlon using AAA118962 at dilution 1:100)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human pancreas using AAA118962 at dilution 1:100)

WB (Western Blot) (All lanes: Myotubularin antibody at 2ug/mlLane 1:Jurkat whole cell lysateLane 2:HepG2 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 70,67 kDaObserved band size: 70 kDa)

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Myotubularin, Polyclonal Antibody (Cat# AAA118962)

Full Name

Rabbit anti-human Myotubularin polyclonal Antibody(MTM1)

Gene Names

MTM1; CNM; MTMX; XLMTM

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purified

Pricing

$235/0.05 mL $345/0.1 mL $665/2x0.1 mL $965/3x0.1 mL $1,255/4x0.1 mL $1,490/5x0.1 mL

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IHC (Immunohistochemisry) (ALDH7A1 was detected in paraffin-embedded sections of human lung cancer tissues using rabbit anti- ALDH7A1 Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method.)

IHC (Immunohiostchemistry) (ALDH7A1 was detected in paraffin-embedded sections of rat brain tissues using rabbit anti- ALDH7A1 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)

WB (Western Blot) (Western blot analysis of ALDH7A1 expression in rat liver extract (lane 1), HEPA whole cell lysates (lane 2) and HELA whole cell lysates (lane 3). ALDH7A1 at 58KD was detected using rabbit anti- ALDH7A1 Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method.)

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ALDH7A1, Polyclonal Antibody (Cat# AAA46821)

Full Name

Anti-ALDH7A1 Antibody

Gene Names

ALDH7A1; EPD; PDE; ATQ1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $2,005/5x0.1 mg

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of Gamma-tubulin staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot) (Western blot analysis of Gamma-tubulin expression in A549 (A), HeLa (B), HEK293T (C), NIH3T3 (D) whole cell lysates.)

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Gamma-tubulin, Polyclonal Antibody (Cat# AAA104846)

Full Name

Anti-Gamma-tubulin Antibody

Gene Names

TUBG1; TUBG; GCP-1; CDCBM4; TUBGCP1

Reactivity

Human, Monkey

Applications

Immunohistochemistry, Western Blot

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

$230/0.03 mL $250/0.05 mL $320/0.1 mL $410/0.2 mL $800/2x0.2 mL $1,160/3x0.2 mL $1,505/4x0.2 mL $1,630/5x0.2 mL

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IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using HER2 (Phospho-Tyr877) antibody (left) or the same antibody preincubated with with blocking peptide (right))

WB (Western Blot) (Western Blot analysis of extracts from A431 cells untreated or treated with EGF using HER2 (Phospho-Tyr877) antibody)

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed MCF7 cells using HER2 (Phospho-Tyr877) antibody)

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HER2, Polyclonal Antibody (Cat# AAA107624)

Full Name

HER2 antibody

Gene Names

ERBB2; HER2; erb2

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

HER2 antibody was purified by immunoaffinity chromatography.

Pricing

$630/0.05 mg $1,220/2x0.05 mg $1,780/3x0.05 mg $2,315/4x0.05 mg $2,615/5x0.05 mg

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WB (Western Blot) (Anti-RSK1 p90 antibody, AAA45384, All Western blottingAll lanes: Anti-RPS6KA1 (AAA45384) at 0.5ug/mlLane 1: A431 Whole Cell Lysate at 40ugLane 2: MCF-7 Whole Cell Lysate at 40ugLane 3: HELA Whole Cell Lysate at 40ugPredicted bind size: 83KDObserved bind size: 83KD)

WB (Western Blot) (Anti-RSK1 p90 antibody, AAA45384, Western blottingLane 1: MCF-7 Cell LysateLane 2: HELA Cell LysateLane 3: K562 Cell LysateLane 4: JURKAT Cell LysateLane 5: SW620 Cell LysateLane 6: RAJI Cell Lysate)

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RSK1 p90, Polyclonal Antibody (Cat# AAA45384)

Full Name

Anti-RSK1 p90 antibody

Gene Names

RPS6KA1; RSK; HU-1; RSK1; MAPKAPK1A

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $705/0.1 mg (Dylight488) $810/0.1 mg (APC) $810/0.1 mg (PE) $810/0.1 mg (iFluor647) $2,005/5x0.1 mg

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of DNAL1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot) (Western blot analysis of DNAL1 expression in HEK293T (A), mouse testis (B), rat kidney (C) whole cell lysates.)

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DNAL1, Polyclonal Antibody (Cat# AAA104541)

Full Name

Anti-DNAL1 Antibody

Gene Names

DNAL1; CILD16; C14orf168

Reactivity

Human, Mouse, Rat

Applications

Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

$230/0.03 mL $250/0.05 mL $320/0.1 mL $410/0.2 mL $800/2x0.2 mL $1,160/3x0.2 mL $1,505/4x0.2 mL $1,630/5x0.2 mL

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FCM/FACS (Flow Cytometry) (ZNF28 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

WB (Western Blot) (ZNF28 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the ZNF28 antibody detected the ZNF28 protein (arrow).)

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ZNF28, Polyclonal Antibody (Cat# AAA283930)

Full Name

ZNF28 Antibody (N-term)

Gene Names

ZNF28; KOX24

Reactivity

Human

Applications

Flow Cytometry, Western Blot

Purity

This antibody is purified through a protein A column, followed by peptide affinity purification.

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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FCM/FACS (Flow Cytometry) (GSTK1 Antibody (Center) flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohiostchemistry) (GSTK1 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GSTK1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot) (Western blot analysis of GSTK1 Antibody (Center) in mouse liver tissue lysates (35ug/lane). GSTK1 (arrow) was detected using the purified Pab.)

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GSTK1, Polyclonal Antibody (Cat# AAA284200)

Full Name

GSTK1 Antibody (Center)

Gene Names

GSTK1; GST; GST13; hGSTK1; GSTK1-1; GST13-13; GST 13-13

Reactivity

Human, mouse

Applications

Immunohistochemistry, Western Blot, Flow Cytometry

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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IF (Immunofluorescence) (Fluorescent confocal image of A2058 cell stained with PROX1 Antibody (C-term). A2058 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PROX1 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). PROX1 immunoreactivity is localized to Nucleus significantly.)

WB (Western Blot) (PROX1 Antibody (K507) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the PROX1 antibody detected the PROX1 protein (arrow).)

WB (Western Blot) (Western blot analysis of PROX1 (arrow) using rabbit polyclonal PROX1 Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PROX1 gene (Lane 2) (Origene Technologies).)

WB (Western Blot) (Western blot analysis of lysates from A2058, rat PC-12 cell line (from left to right), using PROX1 Antibody (C-term). AAA284941 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)

WB (Western Blot) (Western blot analysis of lysate from A2058 cell line, using PROX1 Antibody (C-term). AAA284941 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)

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PROX1, Polyclonal Antibody (Cat# AAA284941)

Full Name

PROX1 Antibody (C-term)

Reactivity

Human (Predicted Reactivity: Mouse)

Applications

Immunofluorescence, Western Blot

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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IHC (Immunohistochemisry) (Anti-GPR45 antibody IHC of human Prostate, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

IHC (Immunohiostchemistry) (Anti-GPR45 antibody IHC of human Brain, Glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

IHC (Immunohistochemistry) (Anti-GPR45 antibody IHC of human neurons and glia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

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GPR45, Polyclonal Antibody (Cat# AAA52290)

Full Name

Rabbit Polyclonal to Human GPR45

Gene Names

GPR45; PSP24; PSP24A; PSP24(ALPHA)

Reactivity

Gibbon, Human, Monkey, Rabbit
Predicted Reactivity: Gorilla, Bat (at least 90% immunogen sequence identity)

Applications

Immunohistochemistry

Purity

Immunoaffinity Purified

Pricing

$665/0.05 mg $1,295/2x0.05 mg $1,890/3x0.05 mg $2,450/4x0.05 mg $2,775/5x0.05 mg

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IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded human esophagus using SERPINA1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human liver cancer using SERPINA1 antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using SERPINA1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

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SERPINA1, Polyclonal Antibody (Cat# AAA37310)

Full Name

SERPINA1 Polyclonal Antibody

Gene Names

SERPINA1; PI; A1A; AAT; PI1; A1AT; PRO2275; alpha1AT

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity Purification

Pricing

$230/0.02 mL $405/0.1 mL $790/2x0.1 mL $1,145/3x0.1 mL $1,485/4x0.1 mL $1,585/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using MAX antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded mouse brain using MAX antibody at dilution of 1:200 (40x lens).)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded rat brain using MAX antibody at dilution of 1:200 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of MCF-7 cells, using MAX antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

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MAX, Polyclonal Antibody (Cat# AAA37315)

Full Name

MAX Polyclonal Antibody

Gene Names

MAX; bHLHd4

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity Purification

Pricing

$230/0.02 mL $405/0.1 mL $790/2x0.1 mL $1,145/3x0.1 mL $1,485/4x0.1 mL $1,585/5x0.1 mL

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using ITGAM antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

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ITGAM, Polyclonal Antibody (Cat# AAA37316)

Full Name

ITGAM Polyclonal Antibody

Gene Names

ITGAM; CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity Purification

Pricing

$230/0.02 mL $405/0.1 mL $790/2x0.1 mL $1,145/3x0.1 mL $1,485/4x0.1 mL $1,585/5x0.1 mL

WB (Western Blot) (Western blot analysis of extracts of HT-29 cells, using SSTR5 antibody (AAA37329) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.)

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SSTR5, Polyclonal Antibody (Cat# AAA37329)

Full Name

SSTR5 Polyclonal Antibody

Gene Names

SSTR5; SS-5-R

Reactivity

Human

Applications

Western Blot

Purity

Affinity Purification

Pricing

$230/0.02 mL $405/0.1 mL $790/2x0.1 mL $1,145/3x0.1 mL $1,485/4x0.1 mL $1,585/5x0.1 mL

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using CPT2 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded human liver cancer using CPT2 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded rat kidney using CPT2 antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using CPT2 antibody at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)

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CPT2, Polyclonal Antibody (Cat# AAA37337)

Full Name

CPT2 Polyclonal Antibody

Gene Names

CPT2; CPT1; IIAE4; CPTASE

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity Purification

Pricing

$230/0.02 mL $405/0.1 mL $790/2x0.1 mL $1,145/3x0.1 mL $1,485/4x0.1 mL $1,585/5x0.1 mL

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IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded human colon using WISP2 Rabbit pAb (AAA37625) at dilution of 1:100 (40x lens).)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded rat testis using WISP2 Rabbit pAb (AAA37625) at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using WISP2 antibody (AAA37625) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 90s.)

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WISP2, Polyclonal Antibody (Cat# AAA37625)

Full Name

WISP2

Gene Names

WISP2; CCN5; CT58; CTGF-L

Applications

Immunohistochemistry, Western Blot

Purity

Affinity purification

Pricing

$230/0.02 mL $405/0.1 mL $790/2x0.1 mL $1,145/3x0.1 mL $1,485/4x0.1 mL $1,585/5x0.1 mL

What are Polyclonal Antibodies?

Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).

Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.

Key Uses of Polyclonal Antibodies

• Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.


• Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.


• ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.


• Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.


• Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.

2. Rigorous Quality Control

All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.

3. Wide Assortment of Antibodies

Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.

4. Highly Purified

Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.

FAQ

1. How are polyclonal antibodies produced?

Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.

2. How do polyclonal antibodies differ from monoclonal antibodies?

Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.

3. How should I store polyclonal antibodies?

Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.