At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded mouse brain using ACHE antibody at dilution of 1:100 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using ACHE antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffinembedded human placenta using Decorin antibody at dilution of 1:200 (40x lens).)
IHC (Immunohistochemisry) (Immunohistochemistry of paraffinembedded rat lung using Decorin antibody at dilution of 1:200 (40x lens).)
IF (Immunofluorescence) (Immunofluorescence analysis of MCF-7 cells using DCN antibody. Blue: DAPI for nuclear staining.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using DCN Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.)
IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using PPP1CB antibody. Blue: DAPI for nuclear staining.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using PPP1CB antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity Purification
Pricing
WB (Western Blot) (Western blot analysis of extracts of mouse liver, using SCTR antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells using LIG1 antibody.)
IF (Immunofluorescence) (Immunofluorescence analysis of MCF-7 cells using LIG1 antibody. Blue: DAPI for nuclear staining.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using LIG1 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using C1QBP antibody. Blue: DAPI for nuclear staining.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using C1QBP antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
IF (Immunofluorescence) (Immunofluorescence analysis of MCF-7 cells using PAX3 antibody. Blue: DAPI for nuclear staining.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using PAX3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using TYMP antibody. Blue: DAPI for nuclear staining.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using TYMP antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
IF (Immunofluorescence) (Immunofluorescence analysis of MCF-7 cells using EIF6 antibody. Blue: DAPI for nuclear staining.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using EIF6 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using SELENBP1 antibody. Blue: DAPI for nuclear staining.)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human colon using SELENBP1 Antibody at dilution of 1:100 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using SELENBP1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.)
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity Purification
Pricing
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using CHGB antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Mouse kidney using POLB antibody (AAA37014) at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human stomach using POLB antibody (AAA37014) at dilution of1:100 (40x lens).)
IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using POLB antibody. Blue: DAPI for nuclear staining.)
WB (Western Blot) (Western blot analysis of extracts fromnormal (control) and POLB knockout(KO) HeLa cells, using POLB antibody(AAA37014) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L)) at 1:10000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 30s)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using POLB antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using SMAD1 antibody. Blue: DAPI for nuclear staining.)
IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded human kidney using SMAD1 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded rat brain using SMAD1 antibody at dilution of 1:100 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using SMAD1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded human oophoroma using SOD2 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded rat heart using SOD2 antibody at dilution of 1:100 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using SOD2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
IF (Immunofluorescence) (Immunofluorescence of IL21R in A431 cells with IL 21 Receptor antibody at 20 μg/mL.Green: IL-21 Receptor Antibody (2469)Blue: DAPI staining)
IF (Immunofluorescence) (Immunofluorescence of IL-21 Receptor in Rat Lung cells with IL-21 Receptor antibody at 20 μg/mL.)
IHC (Immunohiostchemistry) (Immunohistochemistry of IL-21 receptor in rat lung with IL-21 receptor antibody at 10 μg/mL.)
WB (Western Blot) (Western blot analysis of IL-21 receptor expression in human HepG2 cell lysate with IL-21 receptor antibody at (A) 1 and (B) 2 μg /ml.)
IHC (Immunohistochemisry) (ALDH7A1 was detected in paraffin-embedded sections of human lung cancer tissues using rabbit anti- ALDH7A1 Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method.)
IHC (Immunohiostchemistry) (ALDH7A1 was detected in paraffin-embedded sections of rat brain tissues using rabbit anti- ALDH7A1 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)
WB (Western Blot) (Western blot analysis of ALDH7A1 expression in rat liver extract (lane 1), HEPA whole cell lysates (lane 2) and HELA whole cell lysates (lane 3). ALDH7A1 at 58KD was detected using rabbit anti- ALDH7A1 Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method.)
WB (Western Blot) (Figure. Western blot analysis of IFN gamma using anti-IFN gamma antibody (AAA44940).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane : Recombinant Rat IFN gamma Protein 0.5ngAfter Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFN gamma antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IFN gamma at approximately 22KD. The expected band size for IFN gamma is at 22KD.)
IF (Immunofluorescence) (Immunofluorescence of ZGPAT in human brain cells with ZGPAT antibody at 20 μg/mL.)
WB (Western Blot) (Western blot analysis of ZGPAT in SK-N-SH cell lysate with ZGPAT antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
SEC62 Antibody is affinity chromatography purified via peptide column.
Pricing
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using KIFC1 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using UQCRC2 Rabbit pAb (AAA37503) at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon using UQCRC2 Rabbit pAb (AAA37503) at dilution of 1:100(40x lens).)
IP (Immunoprecipitation) (Immunoprecipitation analysis of 200ug extracts of HepG2 cells using 1ug UQCRC2 antibody. Western blot was performed from the immunoprecipitate using UQCRC2 antibody at a dilition of 1:1000.)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human liver injury using UQCRC2 antibody at dilution of 1:100 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using UQCRC2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)
Immunoprecipitation, Immunohistochemistry, Western Blot
Purity
Affinity Purification
Pricing
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using AFAP1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using CASK antibody. Blue: DAPI for nuclear staining.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using CASK antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human stomach using FDXR antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded human esophagus using FDXR antibody at dilution of 1:100 (40x lens).)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human liver cancer using FDXR antibody at dilution of 1:100 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using FDXR antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 30s.)
IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells using GPLD1 antibody. Blue: DAPI for nuclear staining.)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse heart using GPLD1 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded human prostate using GPLD1 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded rat heart using GPLD1 antibody at dilution of 1:100 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using GPLD1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells using ELF5 antibody. Blue: DAPI for nuclear staining.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using ELF5 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using PSMD9 antibody. Blue: DAPI for nuclear staining.)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded rat kidney using PSMD9 antibody at dilution of 1:200 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using PSMD9 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.)
IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using DDX3X antibody. Blue: DAPI for nuclear staining.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using DDX3X Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.)
IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using PPL antibody.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using PPL antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 30s.)
IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using IPO5 antibody. Blue: DAPI for nuclear staining.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using IPO5 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.)
IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using TAC3 antibody. Blue: DAPI for nuclear staining.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using TAC3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 20s.)
IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using MYL3 antibody. Blue: DAPI for nuclear staining.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using MYL3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.)
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity Purification
Pricing
What are Polyclonal Antibodies?
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.
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