Phospho Antibodies

Phospho-specific antibodies’ typical purpose is to enable researchers to detect changes in proteins. They will exclusively bind to the amino acid sequence on a protein that has been phosphorylated (which is both a physical & chemical change) and do not bind to the same amino acid sequence on said protein if it lacks said phosphorylation. This aids in being able to clearly see and understand the data produced from this particular protein modification.

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human brain tissue using STK39 (Phospho-Ser309) antibody (left)or the same antibody preincubated with blocking peptide (right).)

WB (Western Blot) (Western blot analysis of extracts from COLO cells using STK39 (Phospho-Ser309) Antibody.The lane on the right is treated with the antigen-specific peptide.)

Compare (5 max)

STK39, Polyclonal Antibody (Cat# AAA305874)

Full Name

STK39 (Phospho-Ser311) Antibody

Gene Names

STK39; DCHT; PASK; SPAK

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed MCF cells using p70 S6 Kinase(Phospho-Thr421) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p70 S6 Kinase(Phospho-Thr421) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from Jurkat cells untreated(lane 1) or treated with GM-CSF(lane 2) using p70 S6 Kinase(Phospho-Thr421) Antibody.)

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p70 S6 Kinase, Polyclonal Antibody (Cat# AAA305500)

Full Name

p70 S6 Kinase (Phospho-Thr421) Antibody

Gene Names

RPS6KB1; S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts from Hela cells, treated with UV or calf intestinal phosphatase (CIP), using HSP27 (Phospho-Ser82) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP27(Phospho-Ser82) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from HL60 cells untreated or treated with UV using HSP27(Phospho-Ser82) Antibody.)

Compare (5 max)

HSP27, Polyclonal Antibody (Cat# AAA305525)

Full Name

HSP27 (Phospho-Ser82) Antibody

Gene Names

HSPB1; CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using PLC-g1(phospho-Tyr771) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from 3T3 cells untreated or treated with serum using PLC-g1(phospho-Tyr771) Antibody.)

Compare (5 max)

PLC-gamma1, Polyclonal Antibody (Cat# AAA305526)

Full Name

PLC-gamma1 (phospho-Tyr771) Antibody

Gene Names

PLCG1; PLC1; NCKAP3; PLC-II; PLC148; PLCgamma1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using cofilin(Phospho-Ser3) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cofilin(Phospho-Ser3) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from COLO205 cells using cofilin(Phospho-Ser3) Antibody (Lane 2) and the same antibody preincubated with blocking peptide(Lane1).)

Compare (5 max)

cofilin, Polyclonal Antibody (Cat# AAA305537)

Full Name

cofilin (Phospho-Ser3) Antibody

Gene Names

CFL1; CFL; cofilin; HEL-S-15

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ASK1(Phospho-Ser966) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from various cells using ASK1(Phospho-Ser966) Antibody.)

Compare (5 max)

ASK1, Polyclonal Antibody (Cat# AAA305947)

Full Name

ASK1 (Phospho-Ser966) Antibody

Gene Names

MAP3K5; ASK1; MEKK5; MAPKKK5

Reactivity

Human, Mouse, Monkey

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts from Hela cells, treated with calf intestinal phosphatase (CIP), using HSP90B (Phospho-Ser254) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from Hela cells untreated(lane 1) or treated with hot shock(lane 2) using HSP90B(Phospho-Ser254) Antibody.)

Compare (5 max)

HSP90B, Polyclonal Antibody (Cat# AAA305559)

Full Name

HSP90B (Phospho-Ser254) Antibody

Gene Names

HSP90AB1; HSP84; HSPC2; HSPCB; D6S182; HSP90B

Reactivity

Human, Mouse

Applications

Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of 22RV1 HELA cells using Phospho-p16 (S326) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of JK cells using Phospho-p16 (S326) Polyclonal Antibody)

Compare (5 max)

p16, Polyclonal Antibody (Cat# AAA305589)

Full Name

p16 (Phospho-Ser326) Polyclonal Antibody

Gene Names

CDKN2A; ARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human brain tissue using Ephrin B1/B2/B3 (Phospho-Tyr324) antibody (left)or the same antibody preincubated with blocking peptide (right).)

WB (Western Blot) (Western blot analysis of extracts from K562 cells treated with serum using Ephrin B1/B2/B3 (Phospho-Tyr324) Antibody.The lane on the right is treated with the antigen-specific peptide.)

Compare (5 max)

Ephrin B1/B2/B3, Polyclonal Antibody (Cat# AAA305611)

Full Name

Ephrin B1/B2/B3 (Phospho-Tyr324) Antibody

Gene Names

EFNB1; CFND; CFNS; EFB1; EFL3; EPLG2; Elk-L; LERK2

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts from Hela cells, treated with Noc or calf intestinal phosphatase (CIP), using BCL-XL (Phospho-Ser62) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from Hela cells untreated or treated with EGF using BCL-XL (Phospho-Ser62) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BCL-XL (phospho-Ser62) antibody ().)

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed HeLa cells using BCL-XL (phospho-Ser62) antibody (, Red).)

Compare (5 max)

BCL-XL, Polyclonal Antibody (Cat# AAA305618)

Full Name

BCL-XL(Phospho-Ser62) Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

WB (Western Blot) (Western blot analysis of 293 using p-Emt (Y512) antibody.)

Compare (5 max)

Emt, Polyclonal Antibody (Cat# AAA305994)

Full Name

Emt (Phospho-Tyr512) Polyclonal Antibody

Gene Names

ITK; EMT; LYK; LPFS1; PSCTK2

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using MARCKS(Phospho-Ser158) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from 3T3 cells using MARCKS(Phospho-Ser158) Antibody (Lane 2) and the same antibody preincubated with blocking peptide(Lane1).)

Compare (5 max)

MARCKS, Polyclonal Antibody (Cat# AAA306031)

Full Name

MARCKS (Phospho-Ser158) Antibody

Gene Names

MARCKS; MACS; 80K-L; PKCSL; PRKCSL

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of VEC A549 cells using Phospho-AP-1 (T93) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of 293 cells using Phospho-AP-1 (T93) Polyclonal Antibody)

Compare (5 max)

AP-1, Polyclonal Antibody (Cat# AAA306112)

Full Name

AP-1 (Phospho-Thr93) Polyclonal Antibody

Gene Names

JUN; AP1; AP-1; c-Jun

Reactivity

Human, Mouse, Rat

Applications

Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFkB-p105(Phospho-Ser932) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from 293 cells untreated or treated with TNF-a using NFkB-p105(Phospho-Ser932) Antibody.)

Compare (5 max)

NFkappaB-p105/p50, Polyclonal Antibody (Cat# AAA307389)

Full Name

NFkappaB-p105/p50 (Phospho-Ser932) Antibody

Gene Names

NFKB1; p50; KBF1; p105; EBP-1; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts from COS7 cells, treated with UV or calf intestinal phosphatase (CIP), using ATF2 (Phospho-Thr73 or 55) Antibody.)

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using ATF2(Phospho-Thr73 or 55) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATF2(Phospho-Thr73 or 55) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from HepG2 cells untreated or treated with EGF using ATF2(Phospho-Thr71 or 53) Antibody.)

Compare (5 max)

ATF2, Polyclonal Antibody (Cat# AAA307410)

Full Name

ATF2 (Phospho-Thr73 or 55) Antibody

Gene Names

ATF2; HB16; CREB2; TREB7; CREB-2; CRE-BP1

Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed MCF7 cells using AFX(Phospho-Ser197) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AFX(Phospho-Ser197) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from 293 cells untreated or treated with serum using AFX(Phospho-Ser197) Antibody.)

Compare (5 max)

AFX, Polyclonal Antibody (Cat# AAA307418)

Full Name

AFX (Phospho-Ser197) Antibody

Gene Names

FOXO4; AFX; AFX1; MLLT7

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using Tau(Phospho-Ser356) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from mouse brain tissue using Tau(Phospho-Ser356) Antibody and the same antibody preincubated with blocking peptide.)

Compare (5 max)

Tau, Polyclonal Antibody (Cat# AAA306908)

Full Name

Tau (Phospho-Ser356) Antibody

Gene Names

MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Rat Colorectal tissue using EGFR (Phospho-Thr678) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from MDA cells untreated or treated with EGF using EGFR(Phospho-Thr678) Antibody.)

Compare (5 max)

EGFR, Polyclonal Antibody (Cat# AAA306949)

Full Name

EGFR (Phospho-Thr678) Antibody

Gene Names

EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded humanbrain tissue, using MAP2K7 (Phospho-Ser271) antibody (left)or the same antibody preincubated with blocking peptide (right).)

WB (Western Blot) (Western blot analysis of extracts from 3T3 cells treated with insulin using MAP2K7 (Phospho-Ser271) Antibody.The lane on the right is treated with the antigen-specific peptide.)

Compare (5 max)

MAP2K7, Polyclonal Antibody (Cat# AAA307168)

Full Name

MAP2K7 (Phospho-Ser271) Antibody

Gene Names

MAP2K7; MEK; MKK7; JNKK2; MEK 7; MAPKK7; PRKMK7; SAPKK4; SAPKK-4

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts from C6 cells, treated with PMA or calf intestinal phosphatase (CIP), using c-Jun (Phospho-Ser73) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-Jun(Phospho-Ser73) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from PC14 cells untreated or treated with EGF using c-Jun(Phospho-Ser73) Antibody.)

Compare (5 max)

c-Jun, Polyclonal Antibody (Cat# AAA307228)

Full Name

c-Jun (Phospho-Ser73) Antibody

Gene Names

JUN; AP1; AP-1; c-Jun

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of 293T cells using Phospho-p63 (S455) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of 293T cells using Phospho-p63 (S455) Polyclonal Antibody)

Compare (5 max)

p63, Polyclonal Antibody (Cat# AAA307097)

Full Name

p63 (Phospho-Ser455) Polyclonal Antibody

Gene Names

TP63; AIS; KET; LMS; NBP; RHS; p40; p51; p63; EEC3; OFC8; p73H; p73L; SHFM4; TP53L; TP73L; p53CP; TP53CP; B(p51A); B(p51B)

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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<< Previous Next >>

WB (Western Blot) (Western blot analysis of extracts from cos-7 cells (Lane 2), using FANCG (Phospho-Ser383) Antibody. The lane on the left is treated with synthesized peptide)

WB (Western Blot) (Western blot analysis of extracts from K562 cells, using FANCG (Phospho-Ser383) antibody. The lane on the right is treated with the synthesized peptide.)

Compare (5 max)

FANCG, Polyclonal Antibody (Cat# AAA307507)

Full Name

FANCG (Phospho-Ser383) Antibody

Gene Names

FANCG; FAG; XRCC9

Reactivity

Human

Applications

Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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<< Previous Next >>

WB (Western Blot) (Western blot analysis of extracts from PC12 cells, treated with PMA or calf intestinal phosphatase (CIP), using PKCtheta (Phospho-Ser676) Antibody.)

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using PKCth(Phospho-Ser676) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PKCth(Phospho-Ser676) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from PC12 cells untreated or treated with PMA using PKCth(Phospho-Ser676) Antibody.)

Compare (5 max)

PKCtheta, Polyclonal Antibody (Cat# AAA307285)

Full Name

PKCtheta (Phospho-Ser676) Antibody

Gene Names

PRKCQ; PRKCT; nPKC-theta

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human brain tissue using PAK1 (Phospho-Ser204) antibody (left)or the same antibody preincubated with blocking peptide (right).)

WB (Western Blot) (Western blot analysis of extracts from 3T3 cells treated with UV using PAK1 (Phospho-Ser204) Antibody.The lane on the right is treated with the antigen-specific peptide.)

Compare (5 max)

PAK1, Polyclonal Antibody (Cat# AAA307290)

Full Name

PAK1 (Phospho-Ser204) Antibody

Gene Names

PAK1; PAKalpha

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts of various cell lines, using Phospho-Rb-S811 antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cell using Phospho-RB-S811 antibody. Blue: DAPI for nuclear staining.)

Compare (5 max)

RB, Polyclonal Antibody (Cat# AAA307297)

Full Name

RB(Phospho-Ser811) antibody

Gene Names

RB1; RB; pRb; OSRC; pp110; p105-Rb; PPP1R130

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$335/0.05 mL $425/0.1 mL $830/2x0.1 mL $1,205/3x0.1 mL $1,565/4x0.1 mL $1,725/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of Jurkat cells using Phospho-4.1R (Y660) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of JK cells using Phospho-4.1R (Y660) Polyclonal Antibody)

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4.1R (Phospho-Tyr660), Polyclonal Antibody (Cat# AAA307028)

Full Name

4.1R (Phospho-Tyr660) Polyclonal Antibody

Gene Names

EPB41; HE; EL1; 4.1R

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using NF-kappaB p100 (phospho- ser866) antibody ().)

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed HeLa cells using NF-kappaB p100 (phospho-ser866) antibody (, Red).)

WB (Western Blot) (Western blot analysis of extracts from ovary cancer cells using NF-kappaB p100 (phospho-Ser866) antibody ().)

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NFkappaB-p100/p52, Polyclonal Antibody (Cat# AAA307440)

Full Name

NFkappaB-p100/p52(Phospho-Ser866) Antibody

Gene Names

NFKB2; p52; p100; H2TF1; LYT10; CVID10; LYT-10; NF-kB2

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts from C2C12 cells, treated with starve or calf intestinal phosphatase (CIP), using ICAM-1 (Phospho-Tyr512) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from HUVEC cells using ICAM-1(Phospho-Tyr512) Antibody (Lane 2) and the same antibody preincubated with blocking peptide(Lane1).)

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ICAM-1, Polyclonal Antibody (Cat# AAA308298)

Full Name

ICAM-1 (Phospho-Tyr512) Antibody

Gene Names

ICAM1; BB2; CD54; P3.58

Reactivity

Human

Applications

Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using Phospho-ERK1/2 Y222/205 Mouse mAb diluted at 1:200.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma Tissue using Phospho-ERK1/2 Y222/205 Mouse mAb diluted at 1:200)

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ERK 1/2, Monoclonal Antibody (Cat# AAA309603)

Full Name

Phospho-ERK 1/2 (Y222/205)Mouse Monoclonal Antibody (4G3)

Reactivity

Human, Rat, Mouse

Applications

Immunohistochemistry

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

Standard Curve (Sample)

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Phosphorylated (phospho Y705) Signal Transducer and Activator of Transcription 3, ELISA Kit (Cat# AAA247846)

Full Name

Canine Phosphorylated (phospho Y705) Signal Transducer and Activator of Transcription 3 ELISA Kit

Reactivity

Canine

Applications

ELISA

Pricing

$595/48-Strip-Wells $845/96-Strip-Wells $1,640/2x96-Strip-Wells $3,715/5x96-Strip-Wells $7,005/10x96-Strip-Wells

Standard Curve (Sample)

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Phospho Interferon Regulatory Factor 3, ELISA Kit (Cat# AAA247358)

Full Name

Mouse Phospho Interferon Regulatory Factor 3 ELISA Kit

Reactivity

Mouse

Pricing

$595/48-Strip-Wells $845/96-Strip-Wells $1,640/2x96-Strip-Wells $3,715/5x96-Strip-Wells $7,005/10x96-Strip-Wells

Standard Curve (Sample)

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Phospho Interferon Regulatory Factor 3, ELISA Kit (Cat# AAA247560)

Full Name

Chicken Phospho Interferon Regulatory Factor 3 ELISA Kit

Reactivity

Chicken

Pricing

$595/48-Strip-Wells $845/96-Strip-Wells $1,640/2x96-Strip-Wells $3,715/5x96-Strip-Wells $7,005/10x96-Strip-Wells

Standard Curve (Sample)

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Non-phospho (Active) beta-Catenin (Ser33/37/Thr41), ELISA Kit (Cat# AAA247608)

Full Name

Human Non-phospho (Active) beta-Catenin (Ser33/37/Thr41) ELISA Kit

Reactivity

Human

Pricing

$595/48-Strip-Wells $845/96-Strip-Wells $1,640/2x96-Strip-Wells $3,715/5x96-Strip-Wells $7,005/10x96-Strip-Wells

WB (Western Blot) (Western Blot analysis of 3T3 cells using Phospho-Per2 (S662) Polyclonal Antibody)

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Per2, Polyclonal Antibody (Cat# AAA303655)

Full Name

Per2 (Phospho-Ser662) Polyclonal Antibody

Gene Names

PER2; FASPS; FASPS1

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Integrin b1 (Phospho-Thr789) antibody (left)or the same antibody preincubated with blocking peptide (right).)

WB (Western Blot) (Western blot analysis of extracts from HepG2 cells treated with Ca2+ using Integrin b1 (Phospho-Thr789) Antibody.The lane on the right is treated with the antigen-specific peptide.)

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Integrin beta1, Polyclonal Antibody (Cat# AAA303657)

Full Name

Integrin beta1 (Phospho-Thr789) Antibody

Gene Names

ITGB1; CD29; FNRB; MDF2; VLAB; GPIIA; MSK12; VLA-BETA

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using Caveolin-1(Phospho-Tyr14) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from 3T3 cells untreated(lane 1) or treated with H2O2(lane 2) using Caveolin-1(Phospho-Tyr14) Antibody.)

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Caveolin-1, Polyclonal Antibody (Cat# AAA303664)

Full Name

Caveolin-1 (Phospho-Tyr14) Antibody

Gene Names

CAV1; CGL3; PPH3; BSCL3; LCCNS; VIP21; MSTP085

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed MEF cells untreated or treated with LY2904 using FKHR (Phospho-Ser256) Antibody.)

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed MCF7 cells using FKHR(Phospho-Ser256) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using FKHR (Phospho-Ser256) Antibody (left) or the same antibody preincubated with blocking peptide #51115 (right).)

WB (Western Blot) (Western blot analysis of extracts from 293 cells untreated or treated with serum using FKHR(Phospho-Ser256) Antibody.)

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FKHR, Polyclonal Antibody (Cat# AAA303505)

Full Name

FKHR (Phospho-Ser256) Antibody

Gene Names

FOXO1; FKH1; FKHR; FOXO1A

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed HeLa cells using ETS1 (Phospho-Thr38) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using ETS1 (Phospho-Thr38) antibody (left)or the same antibody preincubated with blocking peptide (right).)

WB (Western Blot) (Western blot analysis of extracts from HepG2 cells using ETS1 (Phospho-Thr38) Antibody.The lane on the right is treated with the antigen-specific peptide.)

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ETS1, Polyclonal Antibody (Cat# AAA303514)

Full Name

ETS1 (Phospho-Thr38) Antibody

Gene Names

Ets1; Tpl1; Ets-1; AI196000; AI448617; D230050P06

Reactivity

Human, Mouse

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts from Hela cells, treated with calf intestinal phosphatase (CIP), using MEK1 (Phospho-Ser221) Antibody.)

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using MEK1(Phospho-Ser221) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK1(Phospho-Ser221) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from Hela cells untreated or treated with EGF using MEK1(Phospho-Ser221) Antibody.)

Compare (5 max)

MEK1, Polyclonal Antibody (Cat# AAA303545)

Full Name

MEK1 (Phospho-Ser221) Antibody

Gene Names

MAP2K1; CFC3; MEK1; MKK1; MAPKK1; PRKMK1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

WB (Western Blot) (Western blot analysis of extracts from 293 cells using SOX-9 (Phospho-Ser181) Antibody.The lane on the right is treated with the antigen-specific peptide.)

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SOX-9, Polyclonal Antibody (Cat# AAA303572)

Full Name

SOX-9 (Phospho-Ser181) Antibody

Gene Names

SOX9; CMD1; SRA1; CMPD1; SRXX2; SRXY10

Reactivity

Human, Mouse

Applications

Western Blot

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human brain tissue using SF1 (Phospho-Ser82) antibody (left)or the same antibody preincubated with blocking peptide (right).)

WB (Western Blot) (Western blot analysis of extracts from HUVEC cells treated with anisomycin using SF1 (Phospho-Ser82) Antibody.The lane on the right is treated with the antigen-specific peptide.)

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SF1, Polyclonal Antibody (Cat# AAA304025)

Full Name

SF1 (Phospho-Ser82) Antibody

Gene Names

SF1; BBP; MBBP; ZFM1; ZNF162; D11S636; ZCCHC25

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using MDM2(phospho-Ser166) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MDM2(Phospho-Ser166) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from 293 cells untreated or treated with Hydroxyurea using MDM2(phospho-Ser166) Antibody.)

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MDM2, Polyclonal Antibody (Cat# AAA303921)

Full Name

MDM2 (phospho-Ser166) Antibody

Gene Names

MDM2; HDMX; hdm2; ACTFS

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BAD (Phospho-Ser91/128) antibody (left)or the same antibody preincubated with blocking peptide (right).)

WB (Western Blot) (Western blot analysis of extracts from COS7 cells treated with TNF-a using BAD (Phospho-Ser91/128) Antibody.The lane on the right is treated with the antigen-specific peptide.)

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BAD, Polyclonal Antibody (Cat# AAA303460)

Full Name

BAD (Phospho-Ser91/128) Antibody

Gene Names

BAD; BBC2; BCL2L8

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of NIH-3T3 cells using Phospho-Syk (Y348) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of 3T3 cells using Phospho-Syk (Y348) Polyclonal Antibody)

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Syk, Polyclonal Antibody (Cat# AAA303484)

Full Name

Syk (Phospho-Tyr348) Polyclonal Antibody

Gene Names

SYK; p72-Syk

Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from NIH/3T3 cells treated with EGF 200ng/ml 5', using MER/SKY (Phospho-Tyr749/681) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using MER/SKY (Phospho-Tyr749/681) Antibody. The picture on the right is blocked with the phospho peptide.)

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MER/SKY, Polyclonal Antibody (Cat# AAA316617)

Full Name

MER/SKY (Phospho-Tyr749/681) Antibody

Gene Names

MERTK; MER; RP38; c-Eyk; c-mer; Tyro12

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts from Hela cells, treated with IL-4 or calf intestinal phosphatase (CIP), using STAT6 (Phospho-Thr645) Antibody.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT6(Phospho-Thr645) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

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STAT6, Polyclonal Antibody (Cat# AAA243060)

Full Name

Phospho-STAT6 (Thr645) Antibody

Gene Names

STAT6; STAT6B; STAT6C; D12S1644; IL-4-STAT

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Ezrin(Phospho-Tyr353) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from 293 cells untreated or treated with EGF using Ezrin(Phospho-Tyr353) Antibody.)

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EZR, Polyclonal Antibody (Cat# AAA243064)

Full Name

Phospho-EZR (Tyr353) Antibody

Gene Names

EZR; CVL; CVIL; VIL2

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BAD(Phospho-Ser112) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from cos7 cells untreated or treated with EGF using BAD(Phospho-Ser112) Antibody)

Compare (5 max)

Bad, Polyclonal Antibody (Cat# AAA243065)

Full Name

Phospho-Bad (Ser112) Antibody

Gene Names

Bad; Bbc2; AI325008

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using PKD/PKCm(Phospho-Ser910) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from EGF-treated 293 and PMA-treated HUVEC cells using PKD/PKCm(Phospho-Ser910) Antibody.)

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PRKD1, Polyclonal Antibody (Cat# AAA243076)

Full Name

Phospho-PRKD1 (Ser910) Antibody

Gene Names

PRKD1; PKD; PKCM; PRKCM; PKC-MU

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

What Are Phospho Antibodies?

Protein phosphorylation is a process where a phosphate group is added to certain amino acid residues of a protein – usually serine (S), threonine (T), or tyrosine (Y) - by enzymes called kinases. This process is integral in controlling cellular signaling, cellular growth, and other biological functions.

Our catalog includes a wide range of phospho-specific antibodies that can accurately detect this important marker. They perform strongly in widely-used laboratory applications such as Western blot, flow cytometry, immunohistochemistry, and immunofluorescence microscopy. We value your trust in us and are committed to providing top-quality products and services. All of our antibodies are guaranteed to work for the applications and species indicated on our website & associated product pages.

What Are The Key Applications of Phospho Antibodies?

1. Western Blotting

One of the first steps a researcher can take in utilizing these phospho-specific antibodies, is to check if the antibody works using a technique referred to as “Western blot”. For those unfamiliar, Western Blot aids in showing whether the protein that the antibody recognizes is appearing at the correct/expected size. These phospho-specific antibodies should also be able to detect changes in the target protein’s phosphorylation (on/off state) when cells are stimulated in certain ways.

2. Staining of Fixed Cells (Immunocytochemistry)

Another routine use of these phospho-specific antibodies, is to test if the antibody is able to demonstrate similar performance when used on fixed cells (intact cells that have been preserved) as it did in the Western blot tests. It is an important aspect in many cases to confirm that the antibody works in actual intact cell samples. Ideally, the method used for cellular fixation should be the same as what is used in pathology labs (like using 10% formalin). To check if the antibody works well in tissue sections (FFPE), researchers will often test it on fixed cells that are processed similar to tissue samples.

3. Specificity Tests Using Peptides

In order to make sure that the antibody is only binding to the right target:

• Laboratory technicians will mix the antibody with phospho-peptides (short segments of the protein containing the phosphate group modification).


• If the antibody signal disappears, it is confirmation that it is binding to the correct phosphorylated location.


• A more robust test is to use both the phosphorylated and non-phosphorylated (dephosphorylated) versions of the protein. The antibody should react only with the phosphorylated one.


• Another method sometimes utilized is to treat the sample with an enzyme, such as alkaline phosphatase, that specifically removes phosphate groups. If the antibody signal disappears after this, it also confirms specificity.

4. Genetic Confirmation

As a final step, scientists can genetically manipulate the nucleotide sequence and alter the target protein by removing the exact site where phosphorylation happens. If the antibody no longer appears to detect the modified protein, it is strong evidence supporting the antibody being specific for that phosphorylated site.

Why Buy Phospho Antibodies Through Us?

• The production laboratory adheres to strict and consistent protocols prior to releasing any of these phospho-specific antibodies:


• Standard methods and proper controls in all tests to ensure high quality.


• These antibodies are tested and validated in different cell types and species.


• High quality control criterion to ensure each batch is consistent, so you will obtain reliable results every time.

FAQ

1. What Are Phospho-Specific Antibodies?

Phospho-specific antibodies are made to detect proteins only when they have a phosphate group linked to a specific amino acid residue. This empowers scientists understand if a protein is "turned on" or active, based on its phosphorylation state.

2. How to Detect Phosphorylated Proteins in a Western Blot?

To find out if a protein is phosphorylated using Western blot:

• Use a phospho-specific antibody that binds only to the phosphorylated form of the protein.


• You can also use a “regular” antibody for the same amino acid sequence of the protein that the phospho-specific antibody is binding to (but in this case, this antibody will not bind if there is a phosphate group present) in order to compare how much of it is phosphorylated versus how much is non-phosphorylated (or “total” protein, if the “normal” antibody’s epitopes are non-phospho-site-specific).

3. How to Choose the Best Antibody?

Here are some simple tips to help you pick the right antibody:

• Know your target


• Match your sample characteristics


• Confirm the intended use is appropriate


• Check “host” and “type”


• Check the “quality” of the presented data/images


• Appraise whether the available validation meets your needs