Phospho Antibodies

Phospho-specific antibodies’ typical purpose is to enable researchers to detect changes in proteins. They will exclusively bind to the amino acid sequence on a protein that has been phosphorylated (which is both a physical & chemical change) and do not bind to the same amino acid sequence on said protein if it lacks said phosphorylation. This aids in being able to clearly see and understand the data produced from this particular protein modification.

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WB (Western Blot) (Western blot analysis of extracts from Hela cells, treated with EGF or calf intestinal phosphatase (CIP), using IKK alpha (Phospho-Thr23) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using IKK a(Phospho-Thr23) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from 293 cells untreated or treated with TNF using IKK a(Phospho-Thr23) Antibody.)

Compare (5 max)

IKK alpha, Polyclonal Antibody (Cat# AAA307661)

Full Name

IKK alpha (Phospho-Thr23) Antibody

Gene Names

CHUK; IKK1; IKKA; IKBKA; TCF16; NFKBIKA; IKK-alpha

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using 14-3-3z(Phospho-Ser58) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 14-3-3z(Phospho-Ser58) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from Hela cells untreated or treated with UV using 14-3-3z(Phospho-Ser58) Antibody.)

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14-3-3 zeta, Polyclonal Antibody (Cat# AAA307925)

Full Name

14-3-3 zeta (Phospho-Ser58) Antibody

Gene Names

YWHAZ; HEL4; YWHAD; KCIP-1; HEL-S-3; 14-3-3-zeta

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phosphospecific antibodies were removed by chromatogramphy usin

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using synapsin(Phospho-Ser9) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from Mouse Brain tissue using synapsin(Phospho-Ser9) Antibody.)

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synapsin, Polyclonal Antibody (Cat# AAA308179)

Full Name

synapsin (Phospho-Ser9) Antibody

Gene Names

SYN1; SYNI; SYN1a; SYN1b

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HDAC4/HDAC5/HDAC9(Phospho-Ser246/259/220) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from 293 cells and Hela cells treated with serum using HDAC4/HDAC5/HDAC9 (phospho-Ser246/259/220) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from 293 cells untreated or treated with EGF using HDAC4/HDAC5/HDAC9(phospho-Ser246/259/220) Antibody.)

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HDAC4/HDAC5/HDAC9, Polyclonal Antibody (Cat# AAA308059)

Full Name

HDAC4/HDAC5/HDAC9 (phospho-Ser246/259/220) Antibody

Gene Names

HDAC4; HD4; AHO3; BDMR; HDACA; HA6116; HDAC-4; HDAC-A

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extract from U87 cells transfected with either PKCd siRNA targeting or control siRNA were treated withWnt3a (100 ng ml 1) for 8 h. WB was performed with nuclearlysates of the cells with the beta-catenin (Phospho-Ser715) Antibody.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human primary glioblastoma multiforme (GBM) specimens using beta-catenin (Phospho-Ser715) Antibody.)

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beta-catenin, Polyclonal Antibody (Cat# AAA308080)

Full Name

beta-catenin (Phospho-Ser715) Antibody

Gene Names

CTNNB1; CTNNB; MRD19; armadillo

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

WB (Western Blot) (Western blot analysis of extracts from SW626 cells using b-Catenin(Phospho-Thr41/Ser45) Antibody (Lane 2) and the same antibody preincubated with blocking peptide(Lane1).)

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beta-Catenin, Polyclonal Antibody (Cat# AAA303318)

Full Name

beta-Catenin (Phospho-Thr41/Ser45) Antibody

Gene Names

CTNNB1; CTNNB; MRD19; armadillo

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using Histone H3.1 (Phospho-Ser10) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from SK-BR-3 cells, treated with insulin and EGF, and pretreated with U0126 and LY294002 cells using Histone H3.1 (Phospho-Ser10) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Histone H3.1(Phospho-Ser10) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using Histone H3.1 (Phospho-Ser10) Antibody.)

Compare (5 max)

Histone H3.1, Polyclonal Antibody (Cat# AAA303320)

Full Name

Histone H3.1 (Phospho-Ser10) Antibody

Gene Names

HIST1H3A; H3/A; H3FA

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of HELA A431 cells using Phospho-EphB1/2 (Y594/604) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of Hela cells using Phospho-EphB1/2 (Y594/604) Polyclonal Antibody)

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EphB1/2, Polyclonal Antibody (Cat# AAA303344)

Full Name

EphB1/2 (Phospho-Tyr594/604) Polyclonal Antibody

Gene Names

EPHB1; ELK; NET; Hek6; EPHT2

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts from Hela cells, treated with Anisomycin or calf intestinal phosphatase (CIP), using HSP27 (Phospho-Ser15) Antibody.)

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic staining using HSP27(Phospho-Ser15) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP27(Phospho-Ser15) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from Hela cells untreated or treated with UV using HSP27(Phospho-Ser15) Antibody.)

Compare (5 max)

HSP27, Polyclonal Antibody (Cat# AAA303348)

Full Name

HSP27 (Phospho-Ser15) Antibody

Gene Names

HSPB1; CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using PDK1(Phospho-Ser241) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PDK1(Phospho-Ser241) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from 293 and PC12 cells untreated or treated with EGF using PDK1(Phospho-Ser241) Antibody.)

Compare (5 max)

PDK1, Polyclonal Antibody (Cat# AAA303532)

Full Name

PDK1 (Phospho-Ser241) Antibody

Gene Names

PDPK1; PDK1; PDPK2; PDPK2P; PRO0461

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using eIF4G(Phospho-Ser1232) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from HT29 cells untreated or treated with Anisomycin using eIF4G (phospho-Ser1231) Antibody.)

Compare (5 max)

eIF4G, Polyclonal Antibody (Cat# AAA303544)

Full Name

eIF4G (phospho-Ser1231) Antibody

Gene Names

EIF4G1; P220; EIF4F; EIF4G; EIF4GI; PARK18; EIF-4G1

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK1/MEK2(Phospho-Ser217/Ser221) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from Hela cell untreated or treated with UV MEK1/MEK2(Phospho-Ser217/Ser221) Antibody)

Compare (5 max)

MEK1/MEK2, Polyclonal Antibody (Cat# AAA303634)

Full Name

MEK1/MEK2 (Phospho-Ser217/Ser221) Antibody

Gene Names

MAP2K1; CFC3; MEK1; MKK1; MAPKK1; PRKMK1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed MCF cells using VEGFR2(Phospho-Tyr1214) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using VEGFR2(Phospho-Tyr1214) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from SKOV3 cells using VEGFR2(Phospho-Tyr1214) Antibody (Lane 1) and the same antibody preincubated with blocking peptide(Lane2).)

Compare (5 max)

VEGFR2, Polyclonal Antibody (Cat# AAA303646)

Full Name

VEGFR2 (Phospho-Tyr1214) Antibody

Gene Names

KDR; FLK1; CD309; VEGFR; VEGFR2

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed HepG2 cells using CD227/MUC1 (Phospho-Tyr1229) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CD227/MUC1 (Phospho-Tyr1229) antibody (left)or the same antibody preincubated with blocking peptide (right).)

WB (Western Blot) (Western blot analysis of extracts from HepG2 cells treated with PMA using CD227/MUC1 (Phospho-Tyr1229) Antibody.The lane on the right is treated with the antigen-specific peptide.)

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CD227/MUC1, Polyclonal Antibody (Cat# AAA303731)

Full Name

CD227/MUC1 (Phospho-Tyr1229) Antibody

Gene Names

MUC1; EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; CD227; H23AG; MCKD1; MUC-1; ADMCKD; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rb(Phospho-Ser807) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from 3T3 cells untreated or treated with UV using Rb(Phospho-Ser807) Antibody.)

Compare (5 max)

Rb, Polyclonal Antibody (Cat# AAA303473)

Full Name

Rb (Phospho-Ser807) Antibody

Gene Names

RB1; RB; pRb; OSRC; pp110; p105-Rb; PPP1R130

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CREB(Phospho-Ser129) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from 293 cells untreated(lane 1) or treated with UV(lane 2) using CREB(Phospho-Ser129) Antibody.)

Compare (5 max)

CREB, Polyclonal Antibody (Cat# AAA303874)

Full Name

CREB (Phospho-Ser129) Antibody

Gene Names

CREB1; CREB

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human brain tissue using MER/SKY (Phospho-Tyr749/681) antibody (left)or the same antibody preincubated with blocking peptide (right).)

WB (Western Blot) (Western blot analysis of extracts from 3T3 cells treated with EGF using MER/SKY (Phospho-Tyr749/681) Antibody.The lane on the right is treated with the antigen-specific peptide.)

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MER/SKY, Polyclonal Antibody (Cat# AAA303839)

Full Name

MER/SKY (Phospho-Tyr749/681) Antibody

Gene Names

MERTK; MER; RP38; c-Eyk; c-mer; Tyro12

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using b-Catenin(Phospho-Ser33) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from MCF-7 cells untreated or treated with Calyculin A using b-Catenin(Phospho-Ser33) Antibody.)

Compare (5 max)

beta-Catenin, Polyclonal Antibody (Cat# AAA305918)

Full Name

beta-Catenin (Phospho-Ser33) Antibody

Gene Names

CTNNB1; CTNNB; MRD19; armadillo

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts from 293 cells, treated with Hydroxyurea or calf intestinal phosphatase (CIP), using CDC2 (Phospho-Tyr15) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from 293 cells, treated with Hydroxyurea or calf intestinal phosphatase (CIP), using CDC2 (Phospho-Tyr15) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from Hela cells untreated(lane 1) or treated with UV(lane 2) using CDC2(Phospho-Tyr15) Antibody.)

Compare (5 max)

CDC2, Polyclonal Antibody (Cat# AAA305946)

Full Name

CDC2 (Phospho-Tyr15) Antibody

Gene Names

CDK1; CDC2; CDC28A; P34CDC2

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CDC2(Phospho-Thr161) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from Hela cells untreated(lane 1) or treated with UV(lane 2) using CDC2(Phospho-Thr161) Antibody.)

Compare (5 max)

CDC2, Polyclonal Antibody (Cat# AAA305573)

Full Name

CDC2 (Phospho-Thr161) Antibody

Gene Names

CDK1; CDC2; CDC28A; P34CDC2

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human placenta tissue using PAK1/2 (Phospho-Ser199) antibody (left)or the same antibody preincubated with blocking peptide (right).)

WB (Western Blot) (Western blot analysis of extracts from LOVO cells treated with starved using PAK1/2 (Phospho-Ser199) Antibody.The lane on the right is treated with the antigen-specific peptide.)

Compare (5 max)

PAK1, Polyclonal Antibody (Cat# AAA305509)

Full Name

PAK1 (Phospho-Ser199) Antibody

Gene Names

PAK1; PAKalpha

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts from Rat brain tissue or calf intestinal phosphatase (CIP), using Synapsin (phospho-Ser549) Antibody.)

WB (Western Blot) (Western blot analysis of extract from rat brain and mouse brain tissue using Synapsin(phospho-Ser549) Antibody using)

Compare (5 max)

Synapsin, Polyclonal Antibody (Cat# AAA306032)

Full Name

Synapsin (phospho-Ser549) Antibody

Gene Names

Syn1; Syn-1; Syn1-S

Reactivity

Human, Rat, Mouse

Applications

Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed MCF7 cells using p70 S6 Kinase(Phospho-Ser411) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p70 S6 Kinase(Phospho-Ser411) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from MCF cells untreated or treated with EGF using p70 S6 Kinase(Phospho-Ser411) Antibody.)

Compare (5 max)

p70 S6 Kinase, Polyclonal Antibody (Cat# AAA305707)

Full Name

p70 S6 Kinase (Phospho-Ser411) Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed MCF cells using Estrogen Receptor-a(Phospho-Ser106) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Estrogen Receptor-a(Phospho-Ser106) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from MCF7 cells untreated or treated with EGF using Estrogen Receptor-a(Phospho-Ser106) Antibody.)

Compare (5 max)

Estrogen Receptor-alpha, Polyclonal Antibody (Cat# AAA305846)

Full Name

Estrogen Receptor-alpha (Phospho-Ser106) Antibody

Gene Names

ESR1; ER; ESR; Era; ESRA; ESTRR; NR3A1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using p53 (Phospho-Ser15) Antibody.)

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using p53(Phospho-Ser15) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p53 (Phospho-Ser15) Antibody (left) or the same antibody preincubated with blocking peptide #51094 (right).)

WB (Western Blot) (Western blot analysis of extracts from 293 cells untreated or treated with UV using p53(Phospho-Ser15) Antibody.)

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p53, Polyclonal Antibody (Cat# AAA307019)

Full Name

p53 (Phospho-Ser15) Antibody

Gene Names

TP53; P53; BCC7; LFS1; TRP53

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of Jurkat-UV cells using Phospho-Chk2 (T68) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of HELA cells using Phospho-Chk2 (T68) Polyclonal Antibody)

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Chk2, Polyclonal Antibody (Cat# AAA307026)

Full Name

Chk2 (Phospho-Thr68) Polyclonal Antibody

Gene Names

CHEK2; CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using eIF2alpha (Phospho-Ser49) Antibody.)

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using eIF2a(phospho-Ser49) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eIF2alpha (Phospho-Ser49) Antibody (left) or the same antibody preincubated with blocking peptide #51511 (right).)

WB (Western Blot) (Western blot analysis of extracts from Hela cells untreated or treated with starvation using eIF2a(phospho-Ser49) Antibody.)

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eIF2alpha, Polyclonal Antibody (Cat# AAA307047)

Full Name

eIF2alpha (phospho-Ser49) Antibody

Gene Names

EIF2S1; EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using S6 Ribosomal Protein(Phospho-Ser235) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from PC12 cells untreated or treated with anisomycin using S6 Ribosomal Protein(Phospho-Ser235) Antibody.)

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S6 Ribosomal Protein, Polyclonal Antibody (Cat# AAA307250)

Full Name

S6 Ribosomal Protein (Phospho-Ser235) Antibody

Gene Names

RPS6; S6

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT4(Phospho-Tyr693) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from HepG2 cells untreated or treated with IL-4 using STAT4(Phospho-Tyr693) Antibody.)

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STAT4, Polyclonal Antibody (Cat# AAA307272)

Full Name

STAT4 (Phospho-Tyr693) Antibody

Gene Names

STAT4; SLEB11

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using TrkA(Phospho-Ser791) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from Rat and Mouse brain tissue using TrkA(Phospho-Ser791) Antibody.)

Compare (5 max)

TrkA, Polyclonal Antibody (Cat# AAA307279)

Full Name

TrkA (Phospho-Ser791) Antibody

Gene Names

NTRK1; MTC; TRK; TRK1; TRKA; Trk-A; p140-TrkA

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NF-kappaB p105 (phospho-Ser893) antibody ().)

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed HeLa cells using NF-kappaB p105 (phospho-Ser893) antibody (Red).)

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NFkappaB-p105/p50, Polyclonal Antibody (Cat# AAA307302)

Full Name

NFkappaB-p105/p50(Phospho-Ser893) Antibody

Gene Names

NFKB1; p50; KBF1; p105; EBP-1; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry

Purity

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

WB (Western Blot) (Western blot analysis of extracts from HT29 cells, untreated or treated with UV using Ephrin-B2(Phospho-Tyr330) Antibody.)

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Ephrin-B2, Polyclonal Antibody (Cat# AAA307208)

Full Name

Ephrin-B2 (Phospho-Tyr330) Antibody

Gene Names

EFNB2; HTKL; EPLG5; Htk-L; LERK5

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts of Jurkat cell line, using Phospho-MAX-S11 antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cell using Phospho-MAX-S11 antibody. Blue: DAPI for nuclear staining.)

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MAX, Polyclonal Antibody (Cat# AAA307080)

Full Name

MAX(Phospho-Ser11) antibody

Gene Names

MAX; bHLHd4

Reactivity

Human

Applications

Immunofluorescence, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$335/0.05 mL $425/0.1 mL $830/2x0.1 mL $1,205/3x0.1 mL $1,565/4x0.1 mL $1,725/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts from C2C12 cells, treated with serum or calf intestinal phosphatase (CIP), using AMPKalpha1/AMPKalpha2(Phospho-Thr174/Thr172) Antibody.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using AMPKalpha1/AMPKalpha2(Phospho-Thr174/Thr172) Antibody ().)

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AMPKalpha1/AMPKalpha2, Polyclonal Antibody (Cat# AAA306930)

Full Name

AMPKalpha1/AMPKalpha2(Phospho-Thr183/Thr172) Antibody

Gene Names

PRKAA1; AMPK; AMPKa1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts from HepG2 cells (Lane 2), using DYN1 (Phospho-Ser778) Antibody. The lane on the left is treated with synthesized peptide.)

WB (Western Blot) (Western blot analysis of extracts from Mouse brain cells, using DYN1 (Phospho-Ser778) antibody. The lane on the right is treated with the synthesized peptide.)

Compare (5 max)

DYN1, Polyclonal Antibody (Cat# AAA306972)

Full Name

DYN1 (Phospho-Ser778) Antibody

Gene Names

DNM1; DNM; EIEE31

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PKCd(Phospho-Ser645) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from PC12 cells untreated or treated with PMA using PKCd(Phospho-Ser645) Antibody.)

Compare (5 max)

PKCdelta, Polyclonal Antibody (Cat# AAA307444)

Full Name

PKCdelta (Phospho-Ser645) Antibody

Gene Names

PRKCD; MAY1; PKCD; ALPS3; CVID9; nPKC-delta

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using JAK2(Phospho-Tyr1007) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from HT29 cells untreated or treated with IL-4 using JAK2(Phospho-Tyr1007) Antibody.)

Compare (5 max)

JAK2, Polyclonal Antibody (Cat# AAA307448)

Full Name

JAK2 (Phospho-Tyr1007) Antibody

Gene Names

JAK2; JTK10; THCYT3

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using Rb(Phospho-Ser780) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rb(Phospho-Ser780) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from 3T3 cells untreated or treated with UV using Rb(Phospho-Ser780) Antibody.)

Compare (5 max)

Rb, Polyclonal Antibody (Cat# AAA307496)

Full Name

Rb (Phospho-Ser780) Antibody

Gene Names

RB1; RB; pRb; OSRC; pp110; p105-Rb; PPP1R130

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

WB (Western Blot) (Western blot analysis VHL (Phospho-Ser68) using forskolin treated HeLa whole cell lysates)

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VHL, Polyclonal Antibody (Cat# AAA309137)

Full Name

VHL (Phospho-Ser68) Antibody

Gene Names

VHL; RCA1; VHL1; pVHL; HRCA1

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

WB (Western Blot) (Western blot analysis SGK1 (Phospho-Thr256) using Insulin treated HeLa whole cell lysates)

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SGK1, Polyclonal Antibody (Cat# AAA309138)

Full Name

SGK1 (Phospho-Thr256) Antibody

Gene Names

SGK1; SGK

Reactivity

Human

Applications

Western Blot

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

WB (Western Blot) (Western blot analysis IRF5 (Phospho-Ser437) using PDGF treated NIH-3T3 whole cell lysates)

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IRF5, Polyclonal Antibody (Cat# AAA309139)

Full Name

IRF5 (Phospho-Ser437) Antibody

Gene Names

IRF5; SLEB10

Reactivity

Human, Mouse

Applications

Western Blot

Pricing

$340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

WB (Western Blot) (Western blot analysis Mre11 (Phospho-Ser676) using serum treated COS7 whole cell lysates)

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Mre11, Polyclonal Antibody (Cat# AAA309141)

Full Name

Mre11 (Phospho-Ser676) Antibody

Gene Names

MRE11; ATLD; HNGS1; MRE11A; MRE11B

Reactivity

Human

Applications

Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

WB (Western Blot) (Western blot analysis NPM (Phospho-Ser70) using Na3VO4 treated K562 whole cell lysates)

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NPM, Polyclonal Antibody (Cat# AAA309143)

Full Name

NPM (Phospho-Ser70) Antibody

Gene Names

NPM1; B23; NPM

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of a variety of human tumor tissue using CDC25A(Phospho-Tyr59) Antibody. Reference: Nat. Commun. 7:12431 doi: 10.1038/ncomms12431 (2016).)

WB (Western Blot) (Western blot analysis of extracts from U87 cells, treated with EGF using CDC25A(Phospho-Tyr59) Antibody. Reference: Nat. Commun. 7:12431 doi: 10.1038/ncomms12431 (2016).)

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CDC25A, Polyclonal Antibody (Cat# AAA309126)

Full Name

CDC25A(Phospho-Tyr59) Antibody

Gene Names

CDC25A; CDC25A2

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

WB (Western Blot) (Western blot analysis Ret (Phospho-Tyr1096) using EGF treated HepG2 whole cell lysates)

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Ret, Polyclonal Antibody (Cat# AAA309127)

Full Name

Ret (Phospho-Tyr1096) Antibody

Gene Names

RET; PTC; MTC1; HSCR1; MEN2A; MEN2B; RET51; CDHF12; CDHR16; RET-ELE1

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

WB (Western Blot) (Western blot analysis CAMKK1/2 (Phospho-Ser458/495) using EGF treated 293 whole cell lysates)

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CAMKK1/2, Polyclonal Antibody (Cat# AAA309128)

Full Name

CAMKK1/2 (Phospho-Ser458/495) Antibody

Gene Names

CAMKK1; CAMKKA

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

WB (Western Blot) (Western blot analysis SPHK1 (Phospho-Ser225) using 293 whole cell lysates)

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SPHK1, Polyclonal Antibody (Cat# AAA309136)

Full Name

SPHK1 (Phospho-Ser225) Antibody

Gene Names

SPHK1; SPHK

Applications

Western Blot

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human brain tissue using Shc (phospho-Tyr427) antibody)

WB (Western Blot) (Western blot analysis of extracts from Hela cells treated with Calyculin A (50ng/ml, 15mins), using Shc (phospho-Tyr427) antibody.)

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Shc (Phospho-Tyr427), Polyclonal Antibody (Cat# AAA285113)

Full Name

Shc (Phospho-Tyr427) Antibody

Gene Names

SHC1; SHC; SHCA

Reactivity

Human, mouse

Applications

Immunohistochemistry, Western Blot

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

DB (Dot Blot) (Dot blot analysis of anti-phospho-OCT4-S236 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

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Phospho-OCT4 (S236), Polyclonal Antibody (Cat# AAA285152)

Full Name

Phospho-OCT4 (S236) Antibody

Gene Names

POU5F1; OCT3; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4

Reactivity

Human (Predicted Reactivity: Bovine, Mouse, Pig)

Applications

Dot Blot

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

What Are Phospho Antibodies?

Protein phosphorylation is a process where a phosphate group is added to certain amino acid residues of a protein – usually serine (S), threonine (T), or tyrosine (Y) - by enzymes called kinases. This process is integral in controlling cellular signaling, cellular growth, and other biological functions.

Our catalog includes a wide range of phospho-specific antibodies that can accurately detect this important marker. They perform strongly in widely-used laboratory applications such as Western blot, flow cytometry, immunohistochemistry, and immunofluorescence microscopy. We value your trust in us and are committed to providing top-quality products and services. All of our antibodies are guaranteed to work for the applications and species indicated on our website & associated product pages.

What Are The Key Applications of Phospho Antibodies?

1. Western Blotting

One of the first steps a researcher can take in utilizing these phospho-specific antibodies, is to check if the antibody works using a technique referred to as “Western blot”. For those unfamiliar, Western Blot aids in showing whether the protein that the antibody recognizes is appearing at the correct/expected size. These phospho-specific antibodies should also be able to detect changes in the target protein’s phosphorylation (on/off state) when cells are stimulated in certain ways.

2. Staining of Fixed Cells (Immunocytochemistry)

Another routine use of these phospho-specific antibodies, is to test if the antibody is able to demonstrate similar performance when used on fixed cells (intact cells that have been preserved) as it did in the Western blot tests. It is an important aspect in many cases to confirm that the antibody works in actual intact cell samples. Ideally, the method used for cellular fixation should be the same as what is used in pathology labs (like using 10% formalin). To check if the antibody works well in tissue sections (FFPE), researchers will often test it on fixed cells that are processed similar to tissue samples.

3. Specificity Tests Using Peptides

In order to make sure that the antibody is only binding to the right target:

• Laboratory technicians will mix the antibody with phospho-peptides (short segments of the protein containing the phosphate group modification).


• If the antibody signal disappears, it is confirmation that it is binding to the correct phosphorylated location.


• A more robust test is to use both the phosphorylated and non-phosphorylated (dephosphorylated) versions of the protein. The antibody should react only with the phosphorylated one.


• Another method sometimes utilized is to treat the sample with an enzyme, such as alkaline phosphatase, that specifically removes phosphate groups. If the antibody signal disappears after this, it also confirms specificity.

4. Genetic Confirmation

As a final step, scientists can genetically manipulate the nucleotide sequence and alter the target protein by removing the exact site where phosphorylation happens. If the antibody no longer appears to detect the modified protein, it is strong evidence supporting the antibody being specific for that phosphorylated site.

Why Buy Phospho Antibodies Through Us?

• The production laboratory adheres to strict and consistent protocols prior to releasing any of these phospho-specific antibodies:


• Standard methods and proper controls in all tests to ensure high quality.


• These antibodies are tested and validated in different cell types and species.


• High quality control criterion to ensure each batch is consistent, so you will obtain reliable results every time.

FAQ

1. What Are Phospho-Specific Antibodies?

Phospho-specific antibodies are made to detect proteins only when they have a phosphate group linked to a specific amino acid residue. This empowers scientists understand if a protein is "turned on" or active, based on its phosphorylation state.

2. How to Detect Phosphorylated Proteins in a Western Blot?

To find out if a protein is phosphorylated using Western blot:

• Use a phospho-specific antibody that binds only to the phosphorylated form of the protein.


• You can also use a “regular” antibody for the same amino acid sequence of the protein that the phospho-specific antibody is binding to (but in this case, this antibody will not bind if there is a phosphate group present) in order to compare how much of it is phosphorylated versus how much is non-phosphorylated (or “total” protein, if the “normal” antibody’s epitopes are non-phospho-site-specific).

3. How to Choose the Best Antibody?

Here are some simple tips to help you pick the right antibody:

• Know your target


• Match your sample characteristics


• Confirm the intended use is appropriate


• Check “host” and “type”


• Check the “quality” of the presented data/images


• Appraise whether the available validation meets your needs