Phospho Antibodies

Phospho-specific antibodies’ typical purpose is to enable researchers to detect changes in proteins. They will exclusively bind to the amino acid sequence on a protein that has been phosphorylated (which is both a physical & chemical change) and do not bind to the same amino acid sequence on said protein if it lacks said phosphorylation. This aids in being able to clearly see and understand the data produced from this particular protein modification.

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed MCF cells using Progesterone Receptor(Phospho-Ser190) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Progesterone Receptor(Phospho-Ser190) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from SKOV3 cells untreated(lane 1) or treated with EGF(lane 2) using Progesterone Receptor(Phospho-Ser190) Antibody.)

Compare (5 max)

PGR, Polyclonal Antibody (Cat# AAA243267)

Full Name

Phospho-PGR (Ser190) Antibody

Gene Names

PGR; PR; NR3C3

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed MCF7 cells using HER2(Phospho-Tyr877) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using HER2(Phospho-Tyr877) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from A431 cells untreated or treated with EGF using HER2(Phospho-Tyr877) Antibody.)

Compare (5 max)

ERBB2, Polyclonal Antibody (Cat# AAA243268)

Full Name

Phospho-ERBB2 (Tyr877) Antibody

Gene Names

ERBB2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using p53(Phospho-Ser315) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p53(Phospho-Ser315) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from Hela cells using p53(Phospho-Ser315) Antibody(Lane 2) and the same antibody preincubated with blocking peptide(Lane1).)

Compare (5 max)

TP53, Polyclonal Antibody (Cat# AAA243275)

Full Name

Phospho-TP53 (Ser315) Antibody

Gene Names

TP53; P53; BCC7; LFS1; TRP53

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using LIMK2(Phospho-Thr505) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using LIMK2(Phospho-Thr505) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from Hela cells untreated or treated with PMA using LIMK2(Phospho-Thr505) Antibody.)

Compare (5 max)

LIMK2, Polyclonal Antibody (Cat# AAA243281)

Full Name

Phospho-LIMK2 (Thr505) Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using STAT1(Phospho-Ser727) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT1(Phospho-Ser727) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from Hela cells using STAT1(Phospho-Ser727) Antibody.)

Compare (5 max)

STAT1, Polyclonal Antibody (Cat# AAA243292)

Full Name

Phospho-STAT1 (Ser727) Antibody

Gene Names

STAT1; CANDF7; ISGF-3; STAT91

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p90 RSK (Phospho-Thr573) antibody (left)or the same antibody preincubated with blocking peptide (right).)

WB (Western Blot) (Western blot analysis of extracts from 293 cells treated with UV using p90 RSK (Phospho-Thr573) Antibody.The lane on the right is treated with the antigen-specific peptide.)

Compare (5 max)

RPS6KA1, Polyclonal Antibody (Cat# AAA243189)

Full Name

Phospho-RPS6KA1 (Thr573) Antibody

Gene Names

RPS6KA1; RSK; HU-1; RSK1; MAPKAPK1A

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BAD (Phospho-Ser91/128) antibody (left)or the same antibody preincubated with blocking peptide (right).)

WB (Western Blot) (Western blot analysis of extracts from COS7 cells treated with TNF-a using BAD (Phospho-Ser91/128) Antibody.The lane on the right is treated with the antigen-specific peptide.)

Compare (5 max)

BAD, Polyclonal Antibody (Cat# AAA243196)

Full Name

Phospho-BAD (Ser91/128) Antibody

Gene Names

BAD; BBC2; BCL2L8

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed HuvEc cells using SNAI1 (Phospho-Ser246) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from HT29 cells using SNAI1 (Phospho-Ser246) Antibody.The lane on the right is treated with the antigen-specific peptide.)

Compare (5 max)

SNAI1, Polyclonal Antibody (Cat# AAA243202)

Full Name

Phospho-SNAI1 (Ser246) Antibody

Gene Names

SNAI1; SNA; SNAH; SNAIL; SLUGH2; SNAIL1; dJ710H13.1

Reactivity

Human, Mouse

Applications

Immunofluorescence, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human brain tissue using PAK1 (Phospho-Ser204) antibody (left)or the same antibody preincubated with blocking peptide (right).)

WB (Western Blot) (Western blot analysis of extracts from 3T3 cells treated with UV using PAK1 (Phospho-Ser204) Antibody.The lane on the right is treated with the antigen-specific peptide.)

Compare (5 max)

PAK1, Polyclonal Antibody (Cat# AAA243212)

Full Name

Phospho-PAK1 (Ser204) Antibody

Gene Names

PAK1; PAKalpha

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human brain tissue, using CtBP1 (Phospho-Ser422) antibody (left)or the same antibody preincubated with blocking peptide (right).)

WB (Western Blot) (Western blot analysis of extracts from Jurkat cells treated with TNF using CtBP1 (Phospho-Ser422) Antibody.The lane on the right is treated with the antigen-specific peptide.)

Compare (5 max)

CTBP1, Polyclonal Antibody (Cat# AAA243220)

Full Name

Phospho-CTBP1 (Ser422) Antibody

Gene Names

CTBP1; BARS

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MAPKAPK2 (Phospho-Thr334) antibody. The picture on the right is treated with the synthesized peptide.)

WB (Western Blot) (Western blot analysis of extracts from NIH/3T3 cells, using MAPKAPK2 (Phospho-Thr334) antibody. The lane on the right is treated with the synthesized peptide.)

Compare (5 max)

MAPKAPK2, Polyclonal Antibody (Cat# AAA243231)

Full Name

Phospho-MAPKAPK2 (Thr334) Antibody

Gene Names

MAPKAPK2; MK2; MK-2; MAPKAP-K2

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using Gab1(Phospho-Tyr627) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from 293 cells untreated or treated with EGF using Gab1(Phospho-Tyr627) Antibody.)

Compare (5 max)

GAB1, Polyclonal Antibody (Cat# AAA243141)

Full Name

Phospho-GAB1 (Tyr627) Antibody

Reactivity

Human, Mouse

Applications

Immunofluorescence, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of extracts from 293 cells, treated with calf intestinal phosphatase (CIP), using Lck (phospho-Tyr505) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from JK cells using Lck(phospho-Tyr505) Antibody and the same antibody preincubated with blocking peptide.)

Compare (5 max)

LCK, Polyclonal Antibody (Cat# AAA243159)

Full Name

Phospho-LCK (Tyr505) Antibody

Gene Names

LCK; LSK; YT16; p56lck; pp58lck

Reactivity

Human, Mouse

Applications

Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

Standard Curve (Sample)

Compare (5 max)

Phospho Tau(s396), ELISA Kit (Cat# AAA247696)

Full Name

Mouse Phospho Tau(s396) ELISA Kit

Reactivity

Mouse

Pricing

$595/48-Strip-Wells $845/96-Strip-Wells $1,640/2x96-Strip-Wells $3,715/5x96-Strip-Wells $7,005/10x96-Strip-Wells

< >

<< Previous Next >>

WB (Western Blot) (All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.)

WB (Western Blot) (All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.)

WB (Western Blot) (All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.)

WB (Western Blot) (Western blot analysis of Phospho-PKC alpha (T497) expression in HeLa treated with Calyculin A cell lysate.)

Compare (5 max)

PKC alpha, Monoclonal Antibody (Cat# AAA128118)

Full Name

Anti-Phospho-PKC alpha (T497) Rabbit Monoclonal Antibody

Gene Names

PRKCA; AAG6; PKCA; PRKACA; PKC-alpha

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-chromatography

Pricing

$215/0.03 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,005/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot) (Western blot analysis of anti-Rb Pab in Sk-Br-3 cell line lysate (35ug/lane). Rb(arrow) was detected using the purified Pab.)

Compare (5 max)

Phospho-Rb (S249), Polyclonal Antibody (Cat# AAA287474)

Full Name

Phospho-Rb (S249) Antibody

Gene Names

RB1; RB; pRb; OSRC; pp110; p105-Rb; PPP1R130

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Confocal immunofluorescent analysis of Phospho-TSC2-S1387 Antibody with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

DB (Dot Blot) (Dot blot analysis of Phospho-TSC2-S1387 polyclonal antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration was 0.5ug per ml. P-Pab: phospho-antibody; P-Peptide: phospho-peptide; NP-Peptide: non-phospho-peptide.)

Compare (5 max)

Phospho-TSC2 (S1387), Polyclonal Antibody (Cat# AAA287625)

Full Name

Phospho-TSC2 (S1387) Antibody

Gene Names

TSC2; LAM; TSC4; PPP1R160

Reactivity

Human (Predicted Reactivity: Mouse, Rat)

Applications

Immunofluorescence, Dot Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

< >

<< Previous Next >>

DB (Dot Blot) (Dot blot analysis of anti-INSR-pY1361 Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

IHC (Immunohistochemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Compare (5 max)

Phospho-INSR, Polyclonal Antibody (Cat# AAA290976)

Full Name

Phospho-INSR (Y1361) Antibody

Gene Names

INSR; HHF5; CD220

Reactivity

Human

Applications

Dot Blot, Immunohistochemistry

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

< >

<< Previous Next >>

ICC (Immunocytochemistry) (Immunofluorescent analysis of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyurea-treated 3T3 cells using Phospho-Histone H2A.X (Ser139) mouse mAb (1:400).)

WB (Western Blot) (Western blot detection of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyurea-treated 3T3 cell lysates using Phospho-Histone H2A.X (Ser139) mouse mAb (1:2000 diluted).Predicted band size:15KDa.Observed band size:15KDa.)

Compare (5 max)

Histone H2A.X, Monoclonal Antibody (Cat# AAA290512)

Full Name

Phospho-Histone H2A.X (Ser139) Antibody

Gene Names

H2AFX; H2AX; H2A.X; H2A/X

Reactivity

Mouse

Applications

Immunocytochemistry, Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.)

Compare (5 max)

Bcl-6, Polyclonal Antibody (Cat# AAA293985)

Full Name

Bcl-6 (phospho Ser333) Polyclonal Antibody

Gene Names

BCL6; BCL5; LAZ3; BCL6A; ZNF51; ZBTB27

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

Compare (5 max)

C/EBP alpha, Polyclonal Antibody (Cat# AAA293986)

Full Name

C/EBP alpha (phospho Thr230) Polyclonal Antibody

Gene Names

CEBPA; CEBP; C/EBP-alpha

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

Compare (5 max)

RPA p32, Polyclonal Antibody (Cat# AAA293989)

Full Name

RPA p32 (phospho Ser33) Polyclonal Antibody

Gene Names

RPA2; REPA2; RPA32

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.)

Compare (5 max)

Akt, Polyclonal Antibody (Cat# AAA293990)

Full Name

Akt (phospho Thr308) Polyclonal Antibody

Gene Names

AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

Compare (5 max)

CREB-2, Polyclonal Antibody (Cat# AAA293993)

Full Name

CREB-2 (phospho Ser219) Polyclonal Antibody

Gene Names

ATF4; CREB2; TXREB; CREB-2; TAXREB67

Reactivity

Human, Mouse

Applications

Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

Compare (5 max)

EphA2/5, Polyclonal Antibody (Cat# AAA293994)

Full Name

EphA2/5 (phospho Tyr594) Polyclonal Antibody

Gene Names

EPHA2; ECK; CTPA; ARCC2; CTPP1; CTRCT6

Reactivity

Human, Mouse

Applications

Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.)

Compare (5 max)

FAK, Polyclonal Antibody (Cat# AAA293996)

Full Name

FAK (phospho Tyr397) Polyclonal Antibody

Gene Names

PTK2; FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

WB (Western Blot)

Compare (5 max)

Integrin beta3, Polyclonal Antibody (Cat# AAA293997)

Full Name

Integrin beta3 (phospho Tyr785) Polyclonal Antibody

Gene Names

ITGB3; GT; CD61; GP3A; BDPLT2; GPIIIa; BDPLT16

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)

Compare (5 max)

PR, Polyclonal Antibody (Cat# AAA293999)

Full Name

PR (phospho Ser400) Polyclonal Antibody

Gene Names

PGR; PR; NR3C3

Reactivity

Human

Applications

Immunofluorescence, Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)

Compare (5 max)

BAM32, Polyclonal Antibody (Cat# AAA294003)

Full Name

BAM32 (phospho Tyr139) Polyclonal Antibody

Gene Names

DAPP1; BAM32

Reactivity

Human, Mouse

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

Compare (5 max)

GRB10, Polyclonal Antibody (Cat# AAA294008)

Full Name

GRB10 (phospho Tyr67) Polyclonal Antibody

Gene Names

GRB10; RSS; IRBP; MEG1; GRB-IR; Grb-10

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

Compare (5 max)

Nek9, Polyclonal Antibody (Cat# AAA294010)

Full Name

Nek9 (phospho Thr210) Polyclonal Antibody

Gene Names

NEK9; Nek8; NERCC; NERCC1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

Compare (5 max)

IRS-1, Polyclonal Antibody (Cat# AAA294011)

Full Name

IRS-1 (phospho Ser312) Polyclonal Antibody

Gene Names

IRS1; HIRS-1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemisry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohiostchemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

Compare (5 max)

Cdk2/Cdc2, Polyclonal Antibody (Cat# AAA294024)

Full Name

Cdk2/Cdc2 (phospho Thr160) Polyclonal Antibody

Gene Names

CDK2; p33(CDK2)

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

IHC (Immunohiostchemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

Compare (5 max)

C/EBP alpha, Polyclonal Antibody (Cat# AAA294028)

Full Name

C/EBP alpha (phospho Ser21) Polyclonal Antibody

Gene Names

CEBPA; CEBP; C/EBP-alpha

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)

Compare (5 max)

Filamin 1, Polyclonal Antibody (Cat# AAA294029)

Full Name

Filamin 1 (phospho Ser2152) Polyclonal Antibody

Gene Names

FLNA; FLN; FMD; MNS; OPD; ABPX; CSBS; CVD1; FLN1; NHBP; OPD1; OPD2; XLVD; XMVD; FLN-A; ABP-280

Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunohistochemistry, Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

IHC (Immunohiostchemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

Compare (5 max)

PLC gamma2, Polyclonal Antibody (Cat# AAA294035)

Full Name

PLC gamma2 (phospho Tyr753) Polyclonal Antibody

Gene Names

PLCG2; FCAS3; APLAID

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

IHC (Immunohiostchemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

Compare (5 max)

DOR-1, Polyclonal Antibody (Cat# AAA294041)

Full Name

DOR-1 (phospho Ser363) Polyclonal Antibody

Gene Names

OPRD1; OPRD

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

Compare (5 max)

Dynamin I, Polyclonal Antibody (Cat# AAA294042)

Full Name

Dynamin I (phospho Ser774) Polyclonal Antibody

Gene Names

DNM1; DNM

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)

Compare (5 max)

GRF-1, Polyclonal Antibody (Cat# AAA294046)

Full Name

GRF-1 (phospho Tyr1105) Polyclonal Antibody

Gene Names

ARHGAP35; GRF-1; GRLF1; P190A; P190-A; p190RhoGAP; p190ARhoGAP

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

Compare (5 max)

HSL, Polyclonal Antibody (Cat# AAA294047)

Full Name

HSL (phospho Ser552) Polyclonal Antibody

Gene Names

LIPE; HSL; LHS

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)

Compare (5 max)

Arrestin-beta-1, Polyclonal Antibody (Cat# AAA294048)

Full Name

Arrestin-beta-1 (phospho Ser412) Polyclonal Antibody

Gene Names

ARRB1; ARB1; ARR1

Reactivity

Human, Monkey

Applications

Immunohistochemistry, Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

Compare (5 max)

Troponin I-C, Polyclonal Antibody (Cat# AAA294051)

Full Name

Troponin I-C (phospho Ser22/S23) Polyclonal Antibody

Reactivity

Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)

Compare (5 max)

Krs-1/2, Polyclonal Antibody (Cat# AAA294055)

Full Name

Krs-1/2 (phospho Thr183) Polyclonal Antibody

Gene Names

STK3; KRS1; MST2

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)

Compare (5 max)

NMDAzeta1, Polyclonal Antibody (Cat# AAA294056)

Full Name

NMDAzeta1 (phospho Ser896) Polyclonal Antibody

Gene Names

GRIN1; NR1; MRD8; GluN1; NMDA1; NMDAR1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

Compare (5 max)

p63, Polyclonal Antibody (Cat# AAA294057)

Full Name

p63 (phospho Ser455) Polyclonal Antibody

Gene Names

TP63; AIS; KET; LMS; NBP; RHS; p40; p51; p63; EEC3; OFC8; p73H; p73L; SHFM4; TP53L; TP73L; p53CP; TP53CP; B(p51A); B(p51B)

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)

Application Data (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)

Compare (5 max)

YAP, Polyclonal Antibody (Cat# AAA294060)

Full Name

YAP (phospho Ser127) Polyclonal Antibody

Gene Names

YAP1; YAP; YKI; YAP2; YAP65

Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)

Compare (5 max)

Smad3, Polyclonal Antibody (Cat# AAA294064)

Full Name

Smad3 (phospho Ser208) Polyclonal Antibody

Gene Names

SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

What Are Phospho Antibodies?

Protein phosphorylation is a process where a phosphate group is added to certain amino acid residues of a protein – usually serine (S), threonine (T), or tyrosine (Y) - by enzymes called kinases. This process is integral in controlling cellular signaling, cellular growth, and other biological functions.

Our catalog includes a wide range of phospho-specific antibodies that can accurately detect this important marker. They perform strongly in widely-used laboratory applications such as Western blot, flow cytometry, immunohistochemistry, and immunofluorescence microscopy. We value your trust in us and are committed to providing top-quality products and services. All of our antibodies are guaranteed to work for the applications and species indicated on our website & associated product pages.

What Are The Key Applications of Phospho Antibodies?

1. Western Blotting

One of the first steps a researcher can take in utilizing these phospho-specific antibodies, is to check if the antibody works using a technique referred to as “Western blot”. For those unfamiliar, Western Blot aids in showing whether the protein that the antibody recognizes is appearing at the correct/expected size. These phospho-specific antibodies should also be able to detect changes in the target protein’s phosphorylation (on/off state) when cells are stimulated in certain ways.

2. Staining of Fixed Cells (Immunocytochemistry)

Another routine use of these phospho-specific antibodies, is to test if the antibody is able to demonstrate similar performance when used on fixed cells (intact cells that have been preserved) as it did in the Western blot tests. It is an important aspect in many cases to confirm that the antibody works in actual intact cell samples. Ideally, the method used for cellular fixation should be the same as what is used in pathology labs (like using 10% formalin). To check if the antibody works well in tissue sections (FFPE), researchers will often test it on fixed cells that are processed similar to tissue samples.

3. Specificity Tests Using Peptides

In order to make sure that the antibody is only binding to the right target:

• Laboratory technicians will mix the antibody with phospho-peptides (short segments of the protein containing the phosphate group modification).


• If the antibody signal disappears, it is confirmation that it is binding to the correct phosphorylated location.


• A more robust test is to use both the phosphorylated and non-phosphorylated (dephosphorylated) versions of the protein. The antibody should react only with the phosphorylated one.


• Another method sometimes utilized is to treat the sample with an enzyme, such as alkaline phosphatase, that specifically removes phosphate groups. If the antibody signal disappears after this, it also confirms specificity.

4. Genetic Confirmation

As a final step, scientists can genetically manipulate the nucleotide sequence and alter the target protein by removing the exact site where phosphorylation happens. If the antibody no longer appears to detect the modified protein, it is strong evidence supporting the antibody being specific for that phosphorylated site.

Why Buy Phospho Antibodies Through Us?

• The production laboratory adheres to strict and consistent protocols prior to releasing any of these phospho-specific antibodies:


• Standard methods and proper controls in all tests to ensure high quality.


• These antibodies are tested and validated in different cell types and species.


• High quality control criterion to ensure each batch is consistent, so you will obtain reliable results every time.

FAQ

1. What Are Phospho-Specific Antibodies?

Phospho-specific antibodies are made to detect proteins only when they have a phosphate group linked to a specific amino acid residue. This empowers scientists understand if a protein is "turned on" or active, based on its phosphorylation state.

2. How to Detect Phosphorylated Proteins in a Western Blot?

To find out if a protein is phosphorylated using Western blot:

• Use a phospho-specific antibody that binds only to the phosphorylated form of the protein.


• You can also use a “regular” antibody for the same amino acid sequence of the protein that the phospho-specific antibody is binding to (but in this case, this antibody will not bind if there is a phosphate group present) in order to compare how much of it is phosphorylated versus how much is non-phosphorylated (or “total” protein, if the “normal” antibody’s epitopes are non-phospho-site-specific).

3. How to Choose the Best Antibody?

Here are some simple tips to help you pick the right antibody:

• Know your target


• Match your sample characteristics


• Confirm the intended use is appropriate


• Check “host” and “type”


• Check the “quality” of the presented data/images


• Appraise whether the available validation meets your needs