Phospho Antibodies

Phospho-specific antibodies’ typical purpose is to enable researchers to detect changes in proteins. They will exclusively bind to the amino acid sequence on a protein that has been phosphorylated (which is both a physical & chemical change) and do not bind to the same amino acid sequence on said protein if it lacks said phosphorylation. This aids in being able to clearly see and understand the data produced from this particular protein modification.

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using MEK-2(Phospho-Thr394) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK-2(Phospho-Thr394) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from HepG2 and Hela cells untreated or treated with UV using MEK-2(Phospho-Thr394) Antibody.)

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MAP2K2, Polyclonal Antibody (Cat# AAA243258)

Full Name

Phospho-MAP2K2 (Thr394) Antibody

Gene Names

MAP2K2; CFC4; MEK2; MKK2; MAPKK2; PRKMK2

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using G3BP-1(Phospho-Ser232) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using G3BP-1(Phospho-Ser232) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from 293 cells untreated or treated with starvation using G3BP-1(Phospho-Ser232) Antibody.)

Compare (5 max)

G3BP1, Polyclonal Antibody (Cat# AAA243270)

Full Name

Phospho-G3BP1 (Ser232) Antibody

Gene Names

G3BP1; G3BP; HDH-VIII

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using Pyk2(Phospho-Tyr402) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Pyk2(Phospho-Tyr402) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from 293 cells untreated or treated with Serum using Pyk2(Phospho-Tyr402) Antibody.)

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PTK2B, Polyclonal Antibody (Cat# AAA243298)

Full Name

Phospho-PTK2B (Tyr402) Antibody

Gene Names

PTK2B; PKB; PTK; CAKB; FAK2; PYK2; CADTK; FADK2; RAFTK

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using Src (phospho- Tyr418) antibody.)

WB (Western Blot) (Western blot analysis of extracts from COLO205 cells using Src (Ab-418) antibody (Lane 1 and 2) and Src (phospho-Tyr418) antibody (Lane 3 and 4).)

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SRC, Polyclonal Antibody (Cat# AAA243177)

Full Name

Phospho-SRC (Tyr418) Antibody

Gene Names

SRC; ASV; SRC1; c-SRC; p60-Src

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Synaptotagmin (phospho-Thr202) antibody (left)or the same antibody preincubated with blocking peptide (right).)

WB (Western Blot) (Western blot analysis of extracts from colo cells (Lane 2), using Synaptotagmin (Phospho-Thr202) Antibody. The lane on the left is treated with antigen-specific peptide.)

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SYT1, Polyclonal Antibody (Cat# AAA243182)

Full Name

Phospho-SYT1 (Thr202) Antibody

Gene Names

SYT1; P65; SYT; SVP65

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Estrogen Receptor-alpha (Phospho-Tyr537) antibody (left)or the same antibody preincubated with blocking peptide (right).)

WB (Western Blot) (Western blot analysis of extracts from K562 cells (Lane 2), using Estrogen Receptor-alpha (Phospho-Tyr537) Antibody. The lane on the left is treated with antigen-specific peptide.)

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ESR1, Polyclonal Antibody (Cat# AAA243187)

Full Name

Phospho-ESR1 (Tyr537) Antibody

Gene Names

ESR1; ER; ESR; Era; ESRA; NR3A1

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human brain tissue using FRS2 (Phospho-Tyr436) antibody (left)or the same antibody preincubated with blocking peptide (right).)

WB (Western Blot) (Western blot analysis of extracts from HuvEc cells (Lane 2) and JK cells (Lane 3), using FRS2 (Phospho-Tyr436) Antibody. The lane on the left is treated with antigen-specific peptide.)

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FRS2, Polyclonal Antibody (Cat# AAA243215)

Full Name

Phospho-FRS2 (Tyr436) Antibody

Gene Names

FRS2; SNT; SNT1; FRS2A; SNT-1; FRS2alpha

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Gab2 (Phospho-Ser623) antibody (left)or the same antibody preincubated with blocking peptide (right).)

WB (Western Blot) (Western blot analysis of extracts from 293 cells (Lane 2), HeLa cells (Lane 3) and HepG2 cells (Lane 4), using Gab2 (Phospho-Ser623) Antibody. The lane on the left is treated with antigen-specific peptide.)

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GAB2, Polyclonal Antibody (Cat# AAA243224)

Full Name

Phospho-GAB2 (Ser623) Antibody

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using ABL1/2(phospho-Tyr393/439) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ABL1/2(Phospho-Tyr393/439) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from HL60 cells using ABL1/2(phospho-Tyr393/439) Antibody and the same antibody preincubated with blocking peptide.)

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ABL1/ABL2, Polyclonal Antibody (Cat# AAA243325)

Full Name

Phospho-ABL1/ABL2 (Tyr393/439) Antibody

Gene Names

ABL1; ABL; JTK7; p150; c-ABL; v-abl; bcr/abl

Reactivity

Human, Mouse

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed HeLa cells using NF-kB p65(phospho-Thr435) antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NF-kB p65(phospho-Thr435) antibody.)

WB (Western Blot) (Western blot analysis of extracts from Hela cells untreated or treated with TNFa+CA using NFkB-p65(Phospho-Thr435) Antibody.)

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RELA, Polyclonal Antibody (Cat# AAA243333)

Full Name

Phospho-RELA (Thr435) Antibody

Gene Names

RELA; p65; NFKB3

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts from COS7 cells, treated with UV or calf intestinal phosphatase (CIP), using ATF2 (Phospho-Thr73 or 55) Antibody.)

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using ATF2(Phospho-Thr73 or 55) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATF2(Phospho-Thr73 or 55) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from HepG2 cells untreated or treated with EGF using ATF2(Phospho-Thr71 or 53) Antibody.)

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ATF2, Polyclonal Antibody (Cat# AAA243352)

Full Name

Phospho-ATF2 (Thr73 or 55) Antibody

Gene Names

ATF2; HB16; CREB2; TREB7; CRE-BP1

Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed MEF cells untreated or treated with LY2904 using FKHR (Phospho-Ser256) Antibody.)

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed MCF7 cells using FKHR(Phospho-Ser256) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using FKHR (Phospho-Ser256) Antibody (left) or the same antibody preincubated with blocking peptide (right).)

WB (Western Blot) (Western blot analysis of extracts from 293 cells untreated or treated with serum using FKHR(Phospho-Ser256) Antibody.)

Compare (5 max)

FOXO1, Polyclonal Antibody (Cat# AAA243360)

Full Name

Phospho-FOXO1 (Ser256) Antibody

Gene Names

FOXO1; FKH1; FKHR; FOXO1A

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts from Hela cells, treated with Noc or calf intestinal phosphatase (CIP), using NF-kappa;B-p105/p50(Phospho-Ser337) Antibody.)

WB (Western Blot) (Western blot analysis of extract from HeLa cells, using NF-kappa;B p105/p50 (phospho-Ser337) antibody (Lane 1 and 2).)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using NF-kappa;B p105/p50 (phospho-Ser337) antibody.)

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed HeLa cells using NF-kappa;B p105/p50 (phospho-Ser337) antibody.)

Compare (5 max)

NFKB1, Polyclonal Antibody (Cat# AAA243376)

Full Name

Phospho-NFKB1 (Ser337) Antibody

Gene Names

NFKB1; p50; KBF1; p105; EBP-1; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human Lung carcinoma tissue using NFkappaB-p65 (Phospho-Ser536) Antibody.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFkappaB-p65 (Phospho-Ser536) Antibody.)

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using NFkB-p65(Phospho-Ser536) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFkappaB-p65 (Phospho-Ser536) Antibody (left) or the same antibody preincubated with blocking peptide (right).)

WB (Western Blot) (Western blot analysis of extracts from HepG2 and HL60 cells untreated or treated with TNF using NFkB-p65(Phospho-Ser536) Antibody.)

Compare (5 max)

RELA, Polyclonal Antibody (Cat# AAA243383)

Full Name

Phospho-RELA (Ser536) Antibody

Gene Names

RELA; p65; NFKB3

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts from Hela cells, treated with IFNa or calf intestinal phosphatase (CIP), using STAT1 (Phospho-Tyr701) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from MEF cells untreated or treated with interferon-96)

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STAT1, Polyclonal Antibody (Cat# AAA243055)

Full Name

Phospho-STAT1 (Tyr701) Antibody

Gene Names

STAT1; CANDF7; ISGF-3; STAT91

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts from Hela cells, treated with PMA or calf intestinal phosphatase (CIP), using p90RSK (Phospho-Ser352) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from HUVEC cells using p90RSK(Phospho-Ser352) Antibody(Lane 2) and the same antibody preincubated with blocking peptide(Lane1).)

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RPS6KA1, Polyclonal Antibody (Cat# AAA243083)

Full Name

Phospho-RPS6KA1 (Ser352) Antibody

Gene Names

RPS6KA1; RSK; HU-1; RSK1; MAPKAPK1A

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using b-Catenin(Phospho-Ser37) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from HT29 cells using b-Catenin(Phospho-Ser37) Antibody and the same antibody preincubated with blocking peptide.)

Compare (5 max)

CTNNB1, Polyclonal Antibody (Cat# AAA243119)

Full Name

Phospho-CTNNB1 (Ser37) Antibody

Gene Names

CTNNB1; CTNNB; MRD19; armadillo

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using S6 Ribosomal Protein(Phospho-Ser235) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from PC12 cells untreated or treated with anisomycin using S6 Ribosomal Protein(Phospho-Ser235) Antibody.)

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RPS6, Polyclonal Antibody (Cat# AAA243127)

Full Name

Phospho-RPS6 (Ser235) Antibody

Gene Names

RPS6; S6

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using a-Synuclein(Phospho-Tyr133) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from C2C12 cells untreated or treated with Anisomycin using a-Synuclein(Phospho-Tyr133) Antibody.)

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SNCA, Polyclonal Antibody (Cat# AAA243138)

Full Name

Phospho-SNCA (Tyr133) Antibody

Gene Names

SNCA; PD1; NACP; PARK1; PARK4

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts from 293 cells, treated with starve or calf intestinal phosphatase (CIP), using SHP-2 (Phospho-Tyr580) Antibody.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SHP-2(Phospho-Tyr580) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

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PTPN11, Polyclonal Antibody (Cat# AAA243150)

Full Name

Phospho-PTPN11 (Tyr580) Antibody

Gene Names

PTPN11; CFC; NS1; SHP2; BPTP3; PTP2C; PTP-1D; SH-PTP2; SH-PTP3

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using TrkA(Phospho-Ser791) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from Rat and Mouse brain tissue using TrkA(Phospho-Ser791) Antibody.)

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NTRK1, Polyclonal Antibody (Cat# AAA243152)

Full Name

Phospho-NTRK1 (Ser791) Antibody

Gene Names

NTRK1; MTC; TRK; TRK1; TRKA; Trk-A; p140-TrkA

Reactivity

Human

Applications

Immunofluorescence, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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DB (Dot Blot) (Dot blot analysis of Phospho-ERBB2-Y877 Antibody Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)

WB (Western Blot) (Western blot analysis of extracts from T47D cells, untreated or treated with NRG, 100ng/ml, using phospho-ErBB2(Y877) (left) or ErBB2 antibody(right).)

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Phospho-ERBB2 (Y877), Polyclonal Antibody (Cat# AAA284147)

Full Name

Phospho-ERBB2 (Y877) Antibody

Gene Names

ERBB2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu

Reactivity

Human (Predicted Reactivity: Mouse, Rat)

Applications

Western Blot, Dot Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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IF (Immunofluorescence) (Immunofluorescent analysis of Rabphilin 3A (phospho-hospho-Ser237) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of Rabphilin 3A (phospho-Ser237) staining in human brain formalin fixed paraffin embedded tissue section. The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot) (Western blot analysis of Rabphilin 3A (phospho-Ser237) expression in Raw264.7 Calyculin treated whole cell lysates.)

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Rabphilin 3A (phospho-Ser237), Polyclonal Antibody (Cat# AAA310503)

Full Name

Rabphilin 3A (phospho-Ser237) Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Immunogen affinity chromatography

Pricing

$340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of TYRO3/MERTK (phospho-Tyr749/681) staining in human brain formalin fixed paraffin embedded tissue section. The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot) (Western blot analysis of TYRO3/MERTK (phospho-Tyr749/681) expression in HEK293T, H1688, NIH3T3 EGF-treated whole cell lysates.)

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TYRO3/MERTK (phospho-Tyr749/681), Polyclonal Antibody (Cat# AAA310506)

Full Name

TYRO3/MERTK (phospho-Tyr749/681) Antibody

Gene Names

TYRO3; BYK; Dtk; RSE; Rek; Sky; Tif; Etk-2

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Immunogen affinity chromatography

Pricing

$340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescent analysis of Ezrin/Radixin/Moesin (phospho-Thr567/564/558) staining in SKOV3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of Ezrin/Radixin/Moesin (phospho-Thr567/564/558) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot) (Western blot analysis of Ezrin/Radixin/Moesin (phospho-Thr567/564/558) expression in SKOV3, NIH3T3 whole cell lysates.)

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Ezrin/Radixin/Moesin (phospho-Thr567/564/558), Polyclonal Antibody (Cat# AAA310514)

Full Name

Ezrin/Radixin/Moesin (phospho-Thr567/564/558) Antibody

Gene Names

MSN; HEL70; IMD50

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Immunogen affinity chromatography

Pricing

$340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescent analysis of NEK9 (phospho-Thr210) staining in THP1 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of NEK9 (phospho-Thr210) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot) (Western blot analysis of NEK9 (phospho-Thr210) expression in THP1, HepG2, Hela whole cell lysates.)

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NEK9 (phospho-Thr210), Polyclonal Antibody (Cat# AAA310515)

Full Name

NEK9 (phospho-Thr210) Antibody

Gene Names

NEK9; NC; APUG; NERCC; LCCS10; NERCC1

Reactivity

Human, Mouse

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Immunogen affinity chromatography

Pricing

$340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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Application Data (Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic staining.)

WB (Western Blot) (Western blot analysis of eIF2alpha phosphorylation in untreated (A, C) and lambda protein phosphatase treated (B, D) HEK293 whole cell lysates. Antibodies tested include p-eIF2alpha (Ser 52) (A, B) and eIF2alpha (C, D).)

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eIF-2a, Polyclonal Antibody (Cat# AAA310989)

Full Name

eIF-2a (Phospho-Ser 52) Antibody

Gene Names

EIF2S1; EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Western Blot

Purity

Immunogen affinity purified

Pricing

$365/0.05 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $1,990/5x0.1 mL

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Application Data (Immunoperoxidase staining of formalin-fixed, paraffin-embedded human colon carcinoma tissue showing cytoplasmic staining.)

WB (Western Blot) (Western blot analysis of phosphorylated PKR expression in K-562 (A) and starved K-562 (B) whole cell lysates.)

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PKR, Polyclonal Antibody (Cat# AAA310992)

Full Name

PKR (Phospho-Thr446) Antibody

Gene Names

EIF2AK2; PKR; PRKR; EIF2AK1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Western Blot

Purity

Immunogen affinity purified

Pricing

$365/0.05 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $1,990/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Phospho-PKR (T446) antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Phospho-PKR (T446) on different lysates using anti-Phospho-PKR (T446) antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela treated with Calyculin A and TNF-alpha whole cell lysates Lane 2: Untreated Hela whole cell lysates)

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PKR, Monoclonal Antibody (Cat# AAA311000)

Full Name

PKR (Phospho-T446) Antibody

Gene Names

EIF2AK2; PKR; PRKR; EIF2AK1

Reactivity

Human, Mouse, Rat

Applications

Chromatin immunoprecipitation, Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

ProA affinity purified

Pricing

$365/0.05 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $1,990/5x0.1 mL

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ICC (Immunocytochemistry) (ICC staining phospho-FAK (Y397) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining phospho-FAK (Y397) in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining phospho-FAK (Y397) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

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FAK, Monoclonal Antibody (Cat# AAA311013)

Full Name

FAK (Phospho-Y397) Antibody

Gene Names

PTK2; FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK

Reactivity

Human, Mouse, Rat

Applications

Immunoprecipitation, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Western Blot

Purity

ProA affinity purified

Pricing

$365/0.05 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $1,990/5x0.1 mL

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IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Phospho-STAT1 (S727) antibody. Counter stained with hematoxylin.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-STAT1 (S727) antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Phospho-STAT1 (S727) antibody. Counter stained with hematoxylin.)

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STAT1, Monoclonal Antibody (Cat# AAA311017)

Full Name

STAT1 (Phospho-S727) Antibody

Gene Names

STAT1; CANDF7; ISGF-3; STAT91

Reactivity

Human, Mouse, Rat

Applications

Immunoprecipitation, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Western Blot

Purity

ProA affinity purified

Pricing

$365/0.05 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $1,990/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-Phospho-Raf1 (S259) antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Phospho-Raf1 (S259) on different lysates using anti-Phospho-Raf1 (S259) antibody at 1/1, 000 dilution. Positive control: Lane 1: Human skeletal muscle Lane 2: Hela)

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RAF1, Monoclonal Antibody (Cat# AAA311035)

Full Name

RAF1 (Phospho-S259) Antibody

Gene Names

RAF1; NS5; CRAF; Raf-1; c-Raf

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Western Blot

Purity

ProA affinity purified

Pricing

$365/0.05 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $1,990/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using Cyclin E1 (phospho-Thr395) antibody)

WB (Western Blot) (Western blot analysis of extracts from HeLa cells, treated with Paclitasel (1uM, 60mins), using Cyclin E1 (phospho-Thr395) antibody.)

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Cyclin E1 (Phospho-Thr395), Polyclonal Antibody (Cat# AAA286981)

Full Name

Cyclin E1 (Phospho-Thr395) Antibody

Gene Names

CCNE1; CCNE; pCCNE1

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

WB (Western Blot) (Western blot analysis of lysates from A431 cell line, untreated or treated with EGF, 100ng/ml, using Phospho-HUMAN-ARHGAP35(Y1105) (upper) or Beta-actin (lower).)

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Phospho-ARHGAP35 (Y1105), Polyclonal Antibody (Cat# AAA286816)

Full Name

Phospho-ARHGAP35 (Y1105) antibody

Gene Names

ARHGAP35; GRF-1; GRLF1; P190A; P190-A; p190RhoGAP; p190ARhoGAP

Reactivity

Human (Predicted Reactivity: Mouse, Rat)

Applications

Western Blot

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

DB (Dot Blot) (Dot blot analysis of anti-Phospho-Rb-S780 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

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Phospho-Rb (S780), Polyclonal Antibody (Cat# AAA286679)

Full Name

Phospho-Rb (S780) Antibody

Gene Names

RB1; RB; pRb; OSRC; pp110; p105-Rb; PPP1R130

Reactivity

Human (Predicted Reactivity: Mouse, Rat)

Applications

Immunohistochemistry, Dot Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot) (The anti-Phospho-Bad-S75 Pab is used in Western blot to detect Phospho-Bad-S75 in HL60 tissue lysate)

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Phospho-Bad (S75), Polyclonal Antibody (Cat# AAA288792)

Full Name

Phospho-Bad (S75) Antibody

Gene Names

BAD; BBC2; BCL2L8

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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DB (Dot Blot) (Dot blot analysis of anti-Phospho-Nanog-S71 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

IF (Immunofluorescence) (Fluorescent confocal image of SY5Y cells stained with AAA288713 Phospho-Nanog-S71 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with AAA288713 Phospho-Nanog-S71 primary antibody (1:500, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 uM, 25 min). Phospho-Nanog immunoreactivity is localized very specifically to the nuclei of the SY5Y cells.)

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Phospho-Nanog (S71), Polyclonal Antibody (Cat# AAA288713)

Full Name

Phospho-Nanog (S71) Antibody

Reactivity

Human (Predicted Reactivity: Monkey)

Applications

Dot Blot, Immunofluorescence

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot) (Western blot analysis of anti-Phospho-Caspase-pS257 in liver cell line lysates (35ug/lane). Casp6-pS257(arrow) was detected using the purified Pab.)

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Phospho-Caspase 6 (S257), Polyclonal Antibody (Cat# AAA289223)

Full Name

Phospho-Caspase 6 (S257) Antibody

Gene Names

CASP6; MCH2

Reactivity

Human (Predicted Reactivity: Bovine, Mouse, Rat)

Applications

Immunohistochemistry, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot) (The anti-Phospho-GSK3B-S21/29 Pab is used in Western blot to detect Phospho-GSK3B-S21/29 in mouse thymus tissue lysate.)

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Bi-Phospho-GSK3B (S21/29), Polyclonal Antibody (Cat# AAA289535)

Full Name

Bi-Phospho-GSK3B (S21/29) Antibody

Reactivity

Human, mouse

Applications

Immunohistochemistry, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using VE-Cadherin (Phospho-Tyr731) antibody.)

WB (Western Blot) (Western blot analysis of extracts from HepG2 cells, treated with Na3VO4 (0.3mM, 40mins), using VE-Cadherin (Phospho-Tyr731) antibody.)

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VE-Cadherin (Phospho-Tyr731), Polyclonal Antibody (Cat# AAA289567)

Full Name

VE-Cadherin (Phospho-Tyr731) Antibody

Gene Names

CDH5; 7B4; CD144

Reactivity

Human, mouse

Applications

Immunohistochemistry, Western Blot

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-Jun(Phospho-Thr91) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from 293 cells untreated or treated with UV using c-Jun(Phospho-Thr91) Antibody.)

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c-Jun, Polyclonal Antibody (Cat# AAA302842)

Full Name

c-Jun (Phospho-Thr91) Antibody

Gene Names

JUN; AP1; AP-1; c-Jun

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts from 3T3 cells, treated with serum or calf intestinal phosphatase (CIP), using MARCKS (phospho-Ser170) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using MARCKS(Phospho-Ser170) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from MCF cells untreated or treated with EGF using MARCKS(phospho-Ser170) Antibody.)

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MARCKS, Polyclonal Antibody (Cat# AAA302858)

Full Name

MARCKS (phospho-Ser170) Antibody

Gene Names

MARCKS; MACS; 80K-L; PKCSL; PRKCSL

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using PAK1(Phospho-Thr212) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PAK1(Phospho-Thr212) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from 293 cells untreated or treated with forskolin using PAK1(Phospho-Thr212) Antibody.)

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PAK1, Polyclonal Antibody (Cat# AAA302863)

Full Name

PAK1 (Phospho-Thr212) Antibody

Gene Names

PAK1; PAKalpha

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human heart tissue using PEA-15 (Phospho-Ser104) antibody (left)or the same antibody preincubated with blocking peptide (right).)

WB (Western Blot) (Western blot analysis of extracts from COS cells treated with TNF using PEA-15 (Phospho-Ser104) Antibody.The lane on the right is treated with the antigen-specific peptide.)

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PEA-15, Polyclonal Antibody (Cat# AAA304474)

Full Name

PEA-15 (Phospho-Ser104) Antibody

Gene Names

PEA15; PED; MAT1; HMAT1; MAT1H; PEA-15; HUMMAT1H

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts from 293 cells, treated with UV+serum or calf intestinal phosphatase (CIP), using Histone H2A.X (Phospho-Ser139) Antibody.)

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using Histone H2A.X(Phospho-Ser139) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from HT29 cells untreated(lane 1) or treated with UV(lane 2) using Histone H2A.X(Phospho-Ser139) Antibody.)

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Histone H2A.X, Polyclonal Antibody (Cat# AAA304477)

Full Name

Histone H2A.X (Phospho-Ser139) Antibody

Gene Names

H2AFX; H2AX; H2A.X; H2A/X

Reactivity

Human

Applications

Immunofluorescence, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts from Hela cells, treated with PMA or calf intestinal phosphatase (CIP), using p90RSK (Phospho-Ser352) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from HUVEC cells using p90RSK(Phospho-Ser352) Antibody (Lane 2) and the same antibody preincubated with blocking peptide(Lane1).)

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p90RSK, Polyclonal Antibody (Cat# AAA304036)

Full Name

p90RSK (Phospho-Ser352) Antibody

Gene Names

RPS6KA1; RSK; HU-1; RSK1; p90Rsk; MAPKAPK1A

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using BIK (Phospho-Thr33) antibody (#A0053). The picture on the right is treated with the synthesized peptide.)

WB (Western Blot) (Western blot analysis of extracts from K562 cells, using BIK (Phospho-Thr33) antibody. The lane on the right is treated with the synthesized peptide.)

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BIK, Polyclonal Antibody (Cat# AAA304560)

Full Name

BIK (Phospho-Thr33) Antibody

Gene Names

BIK; BP4; NBK; BIP1

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of MOUSE-INTESTINE cells using Phospho-Lyn (Y508) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of 293 cells using Phospho-Lyn (Y508) Polyclonal Antibody)

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Lyn, Polyclonal Antibody (Cat# AAA304563)

Full Name

Lyn (Phospho-Tyr508) Polyclonal Antibody

Gene Names

LYN; JTK8; p53Lyn; p56Lyn

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed HeLa cells using Chk2 (Phospho-Thr383) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from COS7 cells treated with UV using Chk2 (Phospho-Thr383) Antibody.The lane on the right is treated with the antigen-specific peptide.)

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Chk2, Polyclonal Antibody (Cat# AAA304564)

Full Name

Chk2 (Phospho-Thr383) Antibody

Gene Names

CHEK2; CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

What Are Phospho Antibodies?

Protein phosphorylation is a process where a phosphate group is added to certain amino acid residues of a protein – usually serine (S), threonine (T), or tyrosine (Y) - by enzymes called kinases. This process is integral in controlling cellular signaling, cellular growth, and other biological functions.

Our catalog includes a wide range of phospho-specific antibodies that can accurately detect this important marker. They perform strongly in widely-used laboratory applications such as Western blot, flow cytometry, immunohistochemistry, and immunofluorescence microscopy. We value your trust in us and are committed to providing top-quality products and services. All of our antibodies are guaranteed to work for the applications and species indicated on our website & associated product pages.

What Are The Key Applications of Phospho Antibodies?

1. Western Blotting

One of the first steps a researcher can take in utilizing these phospho-specific antibodies, is to check if the antibody works using a technique referred to as “Western blot”. For those unfamiliar, Western Blot aids in showing whether the protein that the antibody recognizes is appearing at the correct/expected size. These phospho-specific antibodies should also be able to detect changes in the target protein’s phosphorylation (on/off state) when cells are stimulated in certain ways.

2. Staining of Fixed Cells (Immunocytochemistry)

Another routine use of these phospho-specific antibodies, is to test if the antibody is able to demonstrate similar performance when used on fixed cells (intact cells that have been preserved) as it did in the Western blot tests. It is an important aspect in many cases to confirm that the antibody works in actual intact cell samples. Ideally, the method used for cellular fixation should be the same as what is used in pathology labs (like using 10% formalin). To check if the antibody works well in tissue sections (FFPE), researchers will often test it on fixed cells that are processed similar to tissue samples.

3. Specificity Tests Using Peptides

In order to make sure that the antibody is only binding to the right target:

• Laboratory technicians will mix the antibody with phospho-peptides (short segments of the protein containing the phosphate group modification).


• If the antibody signal disappears, it is confirmation that it is binding to the correct phosphorylated location.


• A more robust test is to use both the phosphorylated and non-phosphorylated (dephosphorylated) versions of the protein. The antibody should react only with the phosphorylated one.


• Another method sometimes utilized is to treat the sample with an enzyme, such as alkaline phosphatase, that specifically removes phosphate groups. If the antibody signal disappears after this, it also confirms specificity.

4. Genetic Confirmation

As a final step, scientists can genetically manipulate the nucleotide sequence and alter the target protein by removing the exact site where phosphorylation happens. If the antibody no longer appears to detect the modified protein, it is strong evidence supporting the antibody being specific for that phosphorylated site.

Why Buy Phospho Antibodies Through Us?

• The production laboratory adheres to strict and consistent protocols prior to releasing any of these phospho-specific antibodies:


• Standard methods and proper controls in all tests to ensure high quality.


• These antibodies are tested and validated in different cell types and species.


• High quality control criterion to ensure each batch is consistent, so you will obtain reliable results every time.

FAQ

1. What Are Phospho-Specific Antibodies?

Phospho-specific antibodies are made to detect proteins only when they have a phosphate group linked to a specific amino acid residue. This empowers scientists understand if a protein is "turned on" or active, based on its phosphorylation state.

2. How to Detect Phosphorylated Proteins in a Western Blot?

To find out if a protein is phosphorylated using Western blot:

• Use a phospho-specific antibody that binds only to the phosphorylated form of the protein.


• You can also use a “regular” antibody for the same amino acid sequence of the protein that the phospho-specific antibody is binding to (but in this case, this antibody will not bind if there is a phosphate group present) in order to compare how much of it is phosphorylated versus how much is non-phosphorylated (or “total” protein, if the “normal” antibody’s epitopes are non-phospho-site-specific).

3. How to Choose the Best Antibody?

Here are some simple tips to help you pick the right antibody:

• Know your target


• Match your sample characteristics


• Confirm the intended use is appropriate


• Check “host” and “type”


• Check the “quality” of the presented data/images


• Appraise whether the available validation meets your needs