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Phospho Antibodies

Phospho-specific antibodies’ typical purpose is to enable researchers to detect changes in proteins. They will exclusively bind to the amino acid sequence on a protein that has been phosphorylated (which is both a physical & chemical change) and do not bind to the same amino acid sequence on said protein if it lacks said phosphorylation. This aids in being able to clearly see and understand the data produced from this particular protein modification.


Viewing 3100-3150 of 5299 product results


product-image-AAA312985_IHC13.png IHC (Immunohiostchemistry) (IHC analyses of human HCC samples were performed with the indicated antibodies in the presence or absence of a PCK1 pS90 blocking peptide.)

PCK1, Polyclonal Antibody (Cat# AAA312985)

Full Name
PCK1 (Phospho-Ser90) Antibody
Gene Names
PCK1; PEPCK1; PEPCKC; PEPCK-C
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was affinity-purifiedfrom rabbitantiserum by affinity-chromatography using epitope-specific phosphopeptide.The antibody againstnon-phosphopeptidewas removed by chromatographyusing non-phosphopeptidecorresponding tothephosphorylationsite.
Pricing
product-image-AAA288551_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using Chk1 (Phospho-Ser296) antibody.)

Chk1 (Phospho-Ser296), Polyclonal Antibody (Cat# AAA288551)

Full Name
Chk1 (Phospho-Ser296) Antibody
Gene Names
CHEK1; CHK1
Reactivity
Human, mouse, rat
Applications
Immunohistochemistry, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
product-image-AAA310499_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemical analysis of 5-Lipoxygenase (phospho-Ser272) staining in human skeletal muscle formalin fixed paraffin embedded tissue section. The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

5-Lipoxygenase (phospho-Ser272), Polyclonal Antibody (Cat# AAA310499)

Full Name
5-Lipoxygenase (phospho-Ser272) Antibody
Gene Names
ALOX5; 5-LO; 5LPG; LOG5; 5-LOX
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen affinity chromatography
Pricing
product-image-AAA310508_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemical analysis of Ah Receptor (phospho-Ser36) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

Ah Receptor (phospho-Ser36), Polyclonal Antibody (Cat# AAA310508)

Full Name
Ah Receptor (phospho-Ser36) Antibody
Gene Names
AHR; bHLHe76
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen affinity chromatography
Pricing
product-image-AAA310511_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemical analysis of PRKD1/2/3 (phospho-Ser738/Ser742) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

PRKD1/2/3 (phospho-Ser738/Ser742), Polyclonal Antibody (Cat# AAA310511)

Full Name
PRKD1/2/3 (phospho-Ser738/Ser742) Antibody
Gene Names
PRKD1; PKD; PKCM; CHDED; PRKCM; PKC-MU
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen affinity chromatography
Pricing
product-image-AAA310518_IF11.jpg IF (Immunofluorescence) (Immunofluorescent analysis of HSP20 (phospho-Ser16) staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)

HSP20 (phospho-Ser16), Polyclonal Antibody (Cat# AAA310518)

Full Name
HSP20 (phospho-Ser16) Antibody
Gene Names
HSPB6; HEL55; Hsp20; PPP1R91
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Immunogen affinity chromatography
Pricing
product-image-AAA310519_IF11.jpg IF (Immunofluorescence) (Immunofluorescent analysis of ANAPC1 (phospho-Ser688) staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)

ANAPC1 (phospho-Ser688), Polyclonal Antibody (Cat# AAA310519)

Full Name
ANAPC1 (phospho-Ser688) Antibody
Gene Names
ANAPC1; APC1; MCPR; TSG24
Reactivity
Human, Mouse
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Immunogen affinity chromatography
Pricing
product-image-AAA285801_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Shc (phospho-Tyr349) antibody)

Shc (Phospho-Tyr349), Polyclonal Antibody (Cat# AAA285801)

Full Name
Shc (Phospho-Tyr349) Antibody
Gene Names
SHC1; SHC; SHCA
Reactivity
Human, mouse, rat
Applications
Immunohistochemistry, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
product-image-AAA285545_IHC10.jpg IHC (Immunohistochemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Phospho-CDC25A (T507), Polyclonal Antibody (Cat# AAA285545)

Full Name
Phospho-CDC25A (T507) Antibody
Gene Names
CDC25A; CDC25A2
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rat)
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
product-image-AAA285457_IHC11.jpg IHC (Immunohistochemisry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Phospho-CHK1 (S317), Polyclonal Antibody (Cat# AAA285457)

Full Name
Phospho-CHK1 (S317) Antibody
Gene Names
CHEK1; CHK1
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, Dot Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
product-image-AAA287859_DB13.jpg DB (Dot Blot) (Dot blot analysis of PDX1 Antibody (T11) Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

Phospho-PDX1 (T11), Polyclonal Antibody (Cat# AAA287859)

Full Name
Phospho-PDX1 (T11) Antibody
Gene Names
PDX1; GSF; IPF1; IUF1; IDX-1; MODY4; PDX-1; STF-1; PAGEN1
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Applications
Dot Blot, Immunofluorescence
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
product-image-AAA287914_IHC13.jpg IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Phospho-Caspase 9 (S196), Polyclonal Antibody (Cat# AAA287914)

Full Name
Phospho-Caspase 9 (S196) Antibody
Gene Names
CASP9; MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
product-image-AAA287402_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using p90 RSK (Phospho-Thr573) antibody.)

p90 RSK (Phospho-Thr573), Polyclonal Antibody (Cat# AAA287402)

Full Name
p90 RSK (Phospho-Thr573) Antibody
Gene Names
RPS6KA1; RSK; HU-1; RSK1; p90Rsk; MAPKAPK1A
Reactivity
Human, mouse, rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
product-image-AAA287658_WB15.jpg WB (Western Blot) (Western blot analysis of extracts from Jurkat cells treated with TNF, using HS1 (Phospho-Tyr397) antibody.)

HS1 (Phospho-Tyr397), Antibody (Cat# AAA287658)

Full Name
HS1 (Phospho-Tyr397) Antibody
Gene Names
HCLS1; HS1; p75; CTTNL; lckBP1
Reactivity
Human (Identities = 100%, Positives = 100%)
Applications
Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA289606_DB11.jpg DB (Dot Blot) (Dot blot analysis of anti-hSMAD3-S208 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

Phospho-SMAD3 (S208), Polyclonal Antibody (Cat# AAA289606)

Full Name
Phospho-SMAD3 (S208) Antibody
Gene Names
SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
Reactivity
Human (Predicted Reactivity: Chicken, Mouse, Pig, Rat)
Applications
Dot Blot, Immunofluorescence
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
product-image-AAA314872_IF11.jpg IF (Immunofluorescence) (Immunofluorescent analysis of HeLa cells treated with Calyculin A, using Phospho-GSK3 beta (Ser9) Antibody.)

GSK3beta, Monoclonal Recombinant Antibody (Cat# AAA314872)

Full Name
GSK3beta (Phospho-Ser9) Rabbit mAb
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Affinity-chromatography
Pricing
product-image-AAA314838_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer, using Phospho-eIF2 alpha (Ser51) Antibody.)

eIF2 alpha, Monoclonal Recombinant Antibody (Cat# AAA314838)

Full Name
eIF2 alpha (Phospho-Ser51) Rabbit mAb
Gene Names
EIF2S1; EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Affinity-chromatography
Pricing
product-image-AAA302004_WB15.jpg WB (Western Blot) (Western Blot analysis of 453 AD293 cells using Phospho-PERK (T981) Polyclonal Antibody)

PERK, Polyclonal Antibody (Cat# AAA302004)

Full Name
PERK (Phospho-Thr981) Polyclonal Antibody
Gene Names
EIF2AK3; PEK; WRS; PERK
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
product-image-AAA302015_WB15.jpg WB (Western Blot) (Western blot analysis of extracts from HuvEc cells and K562 cells treated with PMA using C-RAF (Phospho-Ser642) Antibody.The lane on the right is treated with the antigen-specific peptide.)

C-RAF, Polyclonal Antibody (Cat# AAA302015)

Full Name
C-RAF (Phospho-Ser642) Antibody
Gene Names
RAF1; NS5; CRAF; Raf-1; c-Raf; CMD1NN
Reactivity
Human
Applications
Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Pricing
product-image-AAA302025_IF11.jpg IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using cdc25C(Phospho-Ser216) Antibody.)

cdc25C, Polyclonal Antibody (Cat# AAA302025)

Full Name
cdc25C (Phospho-Ser216) Antibody
Gene Names
CDC25C; CDC25; PPP1R60
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
product-image-AAA302182_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using 4E-BP1 (phospho-Thr36) antibody ().)

4E-BP1, Polyclonal Antibody (Cat# AAA302182)

Full Name
4E-BP1(Phospho-Thr36) Antibody
Gene Names
EIF4EBP1; BP-1; 4EBP1; 4E-BP1; PHAS-I
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
product-image-AAA302222_WB13.jpg WB (Western Blot) (Western Blot analysis of 293T AD293 cells using Phospho-PLC b3 (S537) Polyclonal Antibody)

PLC beta3, Polyclonal Antibody (Cat# AAA302222)

Full Name
PLC beta3 (Phospho-Ser537) Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
product-image-AAA302543_IF11.jpg IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using GATA1(Phospho-Ser142) Antibody.)

GATA1, Polyclonal Antibody (Cat# AAA302543)

Full Name
GATA1 (Phospho-Ser142) Antibody
Gene Names
GATA1; GF1; GF-1; NFE1; XLTT; ERYF1; NF-E1; XLANP; XLTDA; GATA-1
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
product-image-AAA302321_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human brain tissue using p47 phox (Phospho-Ser345) antibody (left)or the same antibody preincubated with blocking peptide (right).)

p47 phox, Polyclonal Antibody (Cat# AAA302321)

Full Name
p47 phox (Phospho-Ser345) Antibody
Gene Names
NCF1; NCF1A; NOXO2; p47phox; SH3PXD1A
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
product-image-AAA302342_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using p130 Cas (Phospho-Tyr410) antibody (left)or the same antibody preincubated with blocking peptide (right).)

p130 Cas, Polyclonal Antibody (Cat# AAA302342)

Full Name
p130 Cas (Phospho-Tyr410) Antibody
Gene Names
BCAR1; CAS; CAS1; CASS1; CRKAS; P130Cas
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
product-image-AAA302124_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-kit(Phospho-Tyr936) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

c-kit, Polyclonal Antibody (Cat# AAA302124)

Full Name
c-kit (phospho-Tyr936) Antibody
Gene Names
KIT; PBT; SCFR; C-Kit; CD117
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
product-image-AAA302664_WB10.jpg WB (Western Blot) (Western blot analysis of extracts from 3T3 cells, treated with Anisomycin or calf intestinal phosphatase (CIP), using MKK3 (Phospho-Ser189) Antibody.)

MKK3, Polyclonal Antibody (Cat# AAA302664)

Full Name
MKK3 (Phospho-Ser189) Antibody
Gene Names
MAP2K3; MEK3; MKK3; MAPKK3; PRKMK3; SAPKK2; SAPKK-2
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
product-image-AAA302276_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human brain tissue using CRMP-2 (Phospho-Thr509) antibody (left)or the same antibody preincubated with blocking peptide (right).)

CRMP-2, Polyclonal Antibody (Cat# AAA302276)

Full Name
CRMP-2 (Phospho-Thr509) Antibody
Gene Names
DPYSL2; DRP2; N2A3; CRMP2; DRP-2; ULIP2; CRMP-2; DHPRP2; ULIP-2
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
product-image-AAA302429_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-Jun(Phospho-Ser243) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

c-Jun, Polyclonal Antibody (Cat# AAA302429)

Full Name
c-Jun (Phospho-Ser243) Antibody
Gene Names
JUN; AP1; AP-1; c-Jun
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
product-image-AAA302449_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human brain tissue using Retinoblastoma (Phospho-Ser608) antibody (left)or the same antibody preincubated with blocking peptide (right).)

Retinoblastoma, Polyclonal Antibody (Cat# AAA302449)

Full Name
Retinoblastoma (Phospho-Ser608) Antibody
Gene Names
RB1; RB; pRb; OSRC; pp110; p105-Rb; PPP1R130
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
product-image-AAA303306_WB11.jpg WB (Western Blot) (Western blot analysis of extracts from HL60 cells, treated with TNFa or calf intestinal phosphatase (CIP), using P38 MAPK (Phospho-Thr180) Antibody.)

P38 MAPK, Polyclonal Antibody (Cat# AAA303306)

Full Name
P38 MAPK (Phospho-Thr180) Antibody
Gene Names
MAPK14; RK; p38; CSBP; EXIP; Mxi2; CSBP1; CSBP2; CSPB1; PRKM14; PRKM15; SAPK2A; p38ALPHA
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
product-image-AAA303169_WB15.jpg WB (Western Blot) (Western blot analysis of extracts from Hela cells untreated or treated with UV using S6 Ribosomal Protein(Phospho-Ser235/236) Antibody.)

S6 Ribosomal Protein, Polyclonal Antibody (Cat# AAA303169)

Full Name
S6 Ribosomal Protein (Phospho-Ser235/236) Antibody
Gene Names
RPS6; S6
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
product-image-AAA302989_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from JK cells (Lane 2), using ALOX5 (Phospho-Ser523) Antibody. The lane on the left is treated with synthesized peptide.)

ALOX5, Polyclonal Antibody (Cat# AAA302989)

Full Name
ALOX5 (Phospho-Ser523) Antibody
Gene Names
ALOX5; 5-LO; 5LPG; LOG5; 5-LOX
Applications
Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
product-image-AAA303007_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using beta-catenin (phospho-Tyr333) Antibody.)

beta-catenin, Polyclonal Antibody (Cat# AAA303007)

Full Name
beta-catenin (phospho-Tyr333) Antibody
Gene Names
CTNNB1; CTNNB; MRD19; armadillo
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
product-image-AAA303036_IF11.jpg IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed MCF cells using eIF4E(Phospho-Ser209) Antibody.)

eIF4E, Polyclonal Antibody (Cat# AAA303036)

Full Name
eIF4E (Phospho-Ser209) Antibody
Gene Names
EIF4E; CBP; EIF4F; AUTS19; EIF4E1; eIF-4E; EIF4EL1
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
product-image-AAA303079_IF11.jpg IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed HeLa cells using hnRPD (Phospho-Ser83) Antibody.)

hnRPD, Polyclonal Antibody (Cat# AAA303079)

Full Name
hnRPD (Phospho-Ser83) Antibody
Gene Names
HNRNPD; P37; AUF1; AUF1A; HNRPD; hnRNPD0
Reactivity
Human, Mouse
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
product-image-AAA303131_WB10.jpg WB (Western Blot) (Western blot analysis of extracts from HepG2 cells, treated with Anisomycin or calf intestinal phosphatase (CIP), using Src (Phospho-Tyr529) Antibody.)

Src, Polyclonal Antibody (Cat# AAA303131)

Full Name
Src (Phospho-Tyr529) Antibody
Gene Names
SRC; ASV; SRC1; c-SRC; p60-Src
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
product-image-AAA302915_IF11.jpg IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using CDK6(phospho-Tyr24) Antibody.)

CDK6, Polyclonal Antibody (Cat# AAA302915)

Full Name
CDK6 (phospho-Tyr24) Antibody
Gene Names
CDK6; MCPH12; PLSTIRE
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
product-image-AAA302949_IF13.jpg IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using Androgen Receptor(Phospho-Ser213) Antibody.)

Androgen Receptor, Polyclonal Antibody (Cat# AAA302949)

Full Name
Androgen Receptor (Phospho-Ser213) Antibody
Gene Names
AR; KD; AIS; AR8; TFM; DHTR; SBMA; HYSP1; NR3C4; SMAX1; HUMARA
Reactivity
Human
Applications
Immunofluorescence, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
product-image-AAA302813_IF13.jpg IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using PLCg2(Phospho-Tyr753) Antibody.)

PLCgamma2, Polyclonal Antibody (Cat# AAA302813)

Full Name
PLCgamma2 (Phospho-Tyr753) Antibody
Gene Names
PLCG2; FCAS3; APLAID; PLC-IV; PLC-gamma-2
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
product-image-AAA303394_IF11.jpg IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed A549 cells using ACK1 (Phospho-Tyr284) Antibody.)

ACK1, Polyclonal Antibody (Cat# AAA303394)

Full Name
ACK1 (Phospho-Tyr284) Antibody
Gene Names
TNK2; ACK; ACK1; ACK-1; p21cdc42Hs
Reactivity
Human, Mouse
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
product-image-AAA303422_WB11.jpg WB (Western Blot) (Western blot analysis of extracts from Hela cells, treated with EGF or calf intestinal phosphatase (CIP), using HER2 (Phospho-Tyr1221/Tyr1222) Antibody.)

HER2, Polyclonal Antibody (Cat# AAA303422)

Full Name
HER2 (Phospho-Tyr1221/Tyr1222) Antibody
Gene Names
ERBB2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
Reactivity
Human
Applications
Immunofluorescence, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
product-image-AAA305246_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-Jun(Phospho-Thr93) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

c-Jun, Polyclonal Antibody (Cat# AAA305246)

Full Name
c-Jun (Phospho-Thr93) Antibody
Gene Names
JUN; AP1; AP-1; c-Jun
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
product-image-AAA304955_WB10.jpg WB (Western Blot) (Western blot analysis of extracts from 293 cells, treated with insulin or calf intestinal phosphatase (CIP), using GSK3alpha (Phospho-Ser21) Antibody.)

GSK3alpha, Polyclonal Antibody (Cat# AAA304955)

Full Name
GSK3alpha (Phospho-Ser21) Antibody
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
product-image-AAA305001_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using SRF (Phospho-Ser77) antibody. The picture on the right is treated with the synthesized peptide.)

SRF, Polyclonal Antibody (Cat# AAA305001)

Full Name
SRF (Phospho-Ser77) Antibody
Gene Names
SRF; MCM1
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
product-image-AAA304355_IF11.jpg IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed MCF7 cells using PKCb(phospho-Thr641) antibody(, Red).)

PKCbeta, Polyclonal Antibody (Cat# AAA304355)

Full Name
PKCbeta (Phospho-Thr641) Antibody
Gene Names
PRKCB; PKCB; PRKCB1; PRKCB2; PKC-beta
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Pricing
product-image-AAA305295_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using EGFR(Phospho-Ser1070) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

EGFR, Polyclonal Antibody (Cat# AAA305295)

Full Name
EGFR (Phospho-Ser1070) Antibody
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
product-image-AAA305029_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATF2(Phospho-Ser62 or 44) Antibody.)

ATF2, Polyclonal Antibody (Cat# AAA305029)

Full Name
ATF2 (Phospho-Ser62 or 44) Antibody
Gene Names
ATF2; HB16; CREB2; TREB7; CREB-2; CRE-BP1
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
product-image-AAA305059_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using RIPK2 (Phospho-Ser176) antibody. The picture on the right is treated with the synthesized peptide.)

RIPK2, Polyclonal Antibody (Cat# AAA305059)

Full Name
RIPK2 (Phospho-Ser176) Antibody
Gene Names
RIPK2; CCK; RICK; RIP2; CARD3; GIG30; CARDIAK
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
product-image-AAA305133_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from COS7 cells (Lane 2), using C-RAF (Phospho-Ser301) Antibody. The lane on the left is treated with synthesized peptide.)

C-RAF, Polyclonal Antibody (Cat# AAA305133)

Full Name
C-RAF (Phospho-Ser301) Antibody
Gene Names
RAF1; NS5; CRAF; Raf-1; c-Raf; CMD1NN
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing

What Are Phospho Antibodies?


Protein phosphorylation is a process where a phosphate group is added to certain amino acid residues of a protein – usually serine (S), threonine (T), or tyrosine (Y) - by enzymes called kinases. This process is integral in controlling cellular signaling, cellular growth, and other biological functions.


Our catalog includes a wide range of phospho-specific antibodies that can accurately detect this important marker. They perform strongly in widely-used laboratory applications such as Western blot, flow cytometry, immunohistochemistry, and immunofluorescence microscopy. We value your trust in us and are committed to providing top-quality products and services. All of our antibodies are guaranteed to work for the applications and species indicated on our website & associated product pages.


What Are The Key Applications of Phospho Antibodies?


1. Western Blotting


One of the first steps a researcher can take in utilizing these phospho-specific antibodies, is to check if the antibody works using a technique referred to as “Western blot”. For those unfamiliar, Western Blot aids in showing whether the protein that the antibody recognizes is appearing at the correct/expected size. These phospho-specific antibodies should also be able to detect changes in the target protein’s phosphorylation (on/off state) when cells are stimulated in certain ways.


2. Staining of Fixed Cells (Immunocytochemistry)


Another routine use of these phospho-specific antibodies, is to test if the antibody is able to demonstrate similar performance when used on fixed cells (intact cells that have been preserved) as it did in the Western blot tests. It is an important aspect in many cases to confirm that the antibody works in actual intact cell samples. Ideally, the method used for cellular fixation should be the same as what is used in pathology labs (like using 10% formalin). To check if the antibody works well in tissue sections (FFPE), researchers will often test it on fixed cells that are processed similar to tissue samples.


3. Specificity Tests Using Peptides


In order to make sure that the antibody is only binding to the right target:


  • Laboratory technicians will mix the antibody with phospho-peptides (short segments of the protein containing the phosphate group modification).

  • If the antibody signal disappears, it is confirmation that it is binding to the correct phosphorylated location.

  • A more robust test is to use both the phosphorylated and non-phosphorylated (dephosphorylated) versions of the protein. The antibody should react only with the phosphorylated one.

  • Another method sometimes utilized is to treat the sample with an enzyme, such as alkaline phosphatase, that specifically removes phosphate groups. If the antibody signal disappears after this, it also confirms specificity.


4. Genetic Confirmation


As a final step, scientists can genetically manipulate the nucleotide sequence and alter the target protein by removing the exact site where phosphorylation happens. If the antibody no longer appears to detect the modified protein, it is strong evidence supporting the antibody being specific for that phosphorylated site.


Why Buy Phospho Antibodies Through Us?


  • The production laboratory adheres to strict and consistent protocols prior to releasing any of these phospho-specific antibodies:

  • Standard methods and proper controls in all tests to ensure high quality.

  • These antibodies are tested and validated in different cell types and species.

  • High quality control criterion to ensure each batch is consistent, so you will obtain reliable results every time.


FAQ


1. What Are Phospho-Specific Antibodies?


Phospho-specific antibodies are made to detect proteins only when they have a phosphate group linked to a specific amino acid residue. This empowers scientists understand if a protein is "turned on" or active, based on its phosphorylation state.


2. How to Detect Phosphorylated Proteins in a Western Blot?


To find out if a protein is phosphorylated using Western blot:


  • Use a phospho-specific antibody that binds only to the phosphorylated form of the protein.

  • You can also use a “regular” antibody for the same amino acid sequence of the protein that the phospho-specific antibody is binding to (but in this case, this antibody will not bind if there is a phosphate group present) in order to compare how much of it is phosphorylated versus how much is non-phosphorylated (or “total” protein, if the “normal” antibody’s epitopes are non-phospho-site-specific).


3. How to Choose the Best Antibody?


Here are some simple tips to help you pick the right antibody:


  • Know your target

  • Match your sample characteristics

  • Confirm the intended use is appropriate

  • Check “host” and “type”

  • Check the “quality” of the presented data/images

  • Appraise whether the available validation meets your needs


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