Phospho Antibodies

Phospho-specific antibodies’ typical purpose is to enable researchers to detect changes in proteins. They will exclusively bind to the amino acid sequence on a protein that has been phosphorylated (which is both a physical & chemical change) and do not bind to the same amino acid sequence on said protein if it lacks said phosphorylation. This aids in being able to clearly see and understand the data produced from this particular protein modification.

WB (Western Blot) (Western blot using anti-mouse SphK1 (pS225) on mouse B-cell lysate. Antibody used at 1 ug/ml with phosphorylated blocking peptide (lane A) and without (laneB).)

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phospho-Sphingosine Kinase 1,(pS225), Mouse Reactive, Polyclonal Antibody (Cat# AAA60497)

Full Name

phospho-Sphingosine Kinase 1,(pS225), Mouse Reactive

Gene Names

Sphk1; Sk1; Spk1; 1110006G24Rik

Reactivity

Mouse

Applications

Western Blot

Purity

Purity: Affinity Purified
Purification: Antigen Immunoaffiinity Purification

Pricing

$745/0.1 mg $1,455/2x0.1 mg $2,125/3x0.1 mg $2,755/4x0.1 mg $3,160/5x0.1 mg

Standard Curve (Sample)

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Phospho-Nuclear Factor kappa B, ELISA Kit (Cat# AAA91992)

Full Name

Chicken Phospho-Nuclear Factor kappa B ELISA Kit

Reactivity

Chicken

Pricing

$595/48-Strip-Wells $845/96-Strip-Wells $1,640/2x96-Strip-Wells $3,715/5x96-Strip-Wells $7,005/10x96-Strip-Wells

WB (Western Blot) (Western blot image of human jurkat cells untreated (lanes 1 & 3) or treated with calyculin A (100nM, 30 min.) (lanes 2 and 4). The blot was probed with rabbit polyclonals anti-AIFM1 (C-terminal region) at 1:500 (lanes 1 & 2) and anti-AIFM1 (Ser-116) phospho-specific antibody at 1:1000 (lanes 3 & 4).)

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AIFM1, Polyclonal Antibody (Cat# AAA71569)

Full Name

AIFM1 (Ser-116), phospho-specific Antibody

Gene Names

AIFM1; AIF; CMTX4; COWCK; PDCD8; COXPD6

Reactivity

Human

Applications

Western Blot

Pricing

$505/0.1 mL $985/2x0.1 mL $1,440/3x0.1 mL $1,875/4x0.1 mL $2,100/5x0.1 mL

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ICC (Immunocytochemistry) (Immunocytochemical labeling of beta-Catenin in pervanadate-treated A431 cells. The cells were labeled with mouse monoclonal beta-Catenin (CM1181) or rabbit polyclonal beta-Catenin (Tyr-489) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.)

WB (Western Blot) (Western blot analysis of A431 cells stimulated with pervanadate (1mM) for 30 min (lanes 1, 3, & 5) then treated with akaline phosphatase (lanes 2, 4, & 6). The blot was probed with anti-gamma-Catenin (CM1111), anti-beta-Catenin (Tyr-489) conserved site (CP2961), or anti-beta-Catenin (CM1181).)

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beta-Catenin, Polyclonal Antibody (Cat# AAA71609)

Full Name

beta-Catenin (Tyr-489)[gamma-Catenin (Tyr-480)], phospho-specific Antibody

Gene Names

CTNNB1; CTNNB; MRD19; armadillo

Reactivity

Human, Rat, Mouse

Applications

Immunocytochemistry, Western Blot

Pricing

$505/0.1 mL $985/2x0.1 mL $1,440/3x0.1 mL $1,875/4x0.1 mL $2,100/5x0.1 mL

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ICC (Immunocytochemistry) (Immunocytochemical labeling of phosphorylated beta-Catenin in control and pervanadate-treated A431 cells. The cells were labeled with mouse monoclonal beta-Catenin (CM1181) or rabbit polyclonal beta-Catenin (Tyr-86) or beta-Catenin (Y654) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.)

WB (Western Blot) (Western blot analysis of A431 cells stimulated with pervanadate (1mM) for 30 min (lanes 1, 3, & 5) then treated with akaline phosphatase (lanes 2, 4, & 6). The blot was probed with anti-beta-Catenin (CM1181) (lanes 1 & 2), anti-beta-Catenin (Tyr-654) (CP4021) (lanes 3 & 4), or anti-gamma-Catenin (CM1111) (lanes 5 & 6).)

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beta-Catenin, Polyclonal Antibody (Cat# AAA71610)

Full Name

beta-Catenin (Tyr-654)[gamma-Catenin (Tyr-644)], phospho-specific Antibody

Gene Names

CTNNB1; CTNNB; MRD19; armadillo

Reactivity

Human, Rat, Mouse, Chicken, Fish

Applications

Immunocytochemistry, Western Blot

Pricing

$505/0.1 mL $985/2x0.1 mL $1,440/3x0.1 mL $1,875/4x0.1 mL $2,100/5x0.1 mL

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ICC (Immunocytochemistry) (Immunocytochemical labeling of phosphorylated ERK1 in paraformaldehyde-fixed and NP-40-permeabilized rat A7r5 cells treated with calyculin A. The fixed cells were labeled with mouse monoclonal antibodies to anti-ERK1 (EM2331) and anti-ERK1/2 (Thr-202/Tyr-204) (EM2061). The antibodies were detected using Goat anti-Mouse secondary antibodies conjugated to DyLight 488 (left) and DyLight 594 (right).)

WB (Western Blot) (Western blot analysis of human A431 epithelial cells untreated (lanes 1 & 4) or treated with 100nM calyculin A for 30 min. (lanes 2 & 5) or 100 ng/ml EGF for 60 min. (lanes 3 & 6). The blots were probed with anti-ERK1 (C-terminal region) (lanes 1, 2, & 3) or anti-ERK1/2 (Thr-202/Tyr-204) (lanes 4, 5, & 6).)

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ERK1, Monoclonal Antibody (Cat# AAA71624)

Full Name

ERK1 (Thr-202/Tyr-204)[conserved], phospho-specific Antibody

Gene Names

MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK

Reactivity

Human, Rat, Mouse

Applications

Immunocytochemistry, Western Blot

Purity

Protein A chromatography

Pricing

$550/0.1 mL $1,060/2x0.1 mL $1,550/3x0.1 mL $2,005/4x0.1 mL $2,265/5x0.1 mL

Application Data

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Tyrosine Hydroxylase (Ser40), Polyclonal Antibody (Cat# AAA72806)

Full Name

Anti-Phospho-Ser40 Tyrosine Hydroxylase

Gene Names

Th; The

Reactivity

Many Mammalian/ Many Non-mammalian/

Applications

Immunohistochemistry, Immunofluorescence, Western Blot

Purity

Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)

Pricing

$500/0.1 mL $975/2x0.1 mL $1,430/3x0.1 mL $1,855/4x0.1 mL $2,060/5x0.1 mL

WB (Western Blot) (Western blot of HeLa lysate showing specific immunolabeling of the ~142kD DENND3 protein phosphorylated at Ser554 (control). Phosphospecificity is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that the lysate was incubated in lambda-Ptase (800 units/1mg protein for 30 min). The immunolabeling is completely eliminated by treatment with lambda-Ptase.)

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Ser554 DENND3, Polyclonal Antibody (Cat# AAA72827)

Full Name

Anti-Phospho-Ser554 DENND3

Reactivity

Human, Mouse, Non-Human Primate, Rat, Sheep

Applications

Western Blot

Purity

Affinity purified rabbit polyclonal antibody

Pricing

$500/0.1 mL $975/2x0.1 mL $1,430/3x0.1 mL $1,855/4x0.1 mL $2,060/5x0.1 mL

Application Data

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CREB (Ser133), Polyclonal Antibody (Cat# AAA72783)

Full Name

Anti-Phospho-Ser133 CREB

Gene Names

Creb1; Creb

Reactivity

Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)

Pricing

$500/0.1 mL $975/2x0.1 mL $1,430/3x0.1 mL $1,855/4x0.1 mL $2,060/5x0.1 mL

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Application Data

Application Data

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Cofilin 1 (Ser-3), Polyclonal Antibody (Cat# AAA71517)

Full Name

Cofilin 1 (Ser-3), phospho-specific

Gene Names

CFL1; CFL

Reactivity

Human, Rat, Mouse

Applications

Western Blot

Purity

Affinity Purified

Pricing

$505/0.1 mL $985/2x0.1 mL $1,440/3x0.1 mL $1,875/4x0.1 mL $2,100/5x0.1 mL

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ICC (Immunocytochemistry) (Immunocytochemical labeling using anti-Actin (N-terminal) and anti-Actin (Tyr-53) polyclonal antibodies in C2C12 cells control (left) or treated with pervanadate (1 mM) for 30 min (middle). The cells were fixed in paraformaldehyde and permeabilized in acetone. Both antibodies were used in the presence of blocking peptide: Actin (N-terminal) peptide or phospho-Actin (Tyr-53) peptide, respectively (right).)

WB (Western Blot) (Western blot analysis of mouse C2C12 cells untreated (lanes 1 & 3), or treated with pervanadate (1 mM) for 30 min (lanes 2 & 4). The blot was probed with anti-Actin (N-terminal) antibody (lanes 1 & 2) or anti-Actin (Tyr-53) antibody (lanes 3 & 4).)

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Actin, Polyclonal Antibody (Cat# AAA71548)

Full Name

Actin (Tyr-53), Phospho-Specific

Gene Names

ACTB; BRWS1; PS1TP5BP1

Reactivity

Human, Rat, Mouse, Chicken

Applications

Immunocytochemistry, Western Blot

Purity

Affinity Purified

Pricing

$505/0.1 mL $985/2x0.1 mL $1,440/3x0.1 mL $1,875/4x0.1 mL $2,100/5x0.1 mL

Application Data

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IRS-1 (pS312),, Antibody (Cat# AAA71386)

Full Name

Rabbit anti Phospho- IRS-1 (pSer 312) antibody

Gene Names

IRS1; HIRS-1

Reactivity

Human, Rat, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

The Rabbit IgG is purified by Site-specific Epitope Affinity Purification.

Pricing

$365/0.1 mg $705/2x0.1 mg $1,030/3x0.1 mg $1,335/4x0.1 mg $1,420/5x0.1 mg

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WB (Western Blot) (Left: Western blot image of HUVEC cells untreated (-) or treated with pervanadate (1mM) for 30 min. (+). Right: Western blot image of GST-recombinant VEGFR-2 kinase without (-) or with (+) akaline phosphatase treatment. Both sets of blots were probed with rabbit polyclonal anti-VEGFR-2 (a.a. 1304-1317) or anti-VEGFR-2 (Tyr-801).)

WB (Western Blot) (Western blot image of GST-recombinant human VEGFR-1 (89kDa), VEGFR-2 (110kDa), and VEGFR-3 (86kDa) C-terminal regions. The blots were probed with rabbit polyclonal anti-VEGFR-2 (a.a. 1304-1317), anti-VEGFR-2 (Tyr-801, conserved site), and anti-VEGFR-3 (a.a. 1285-1298).)

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VEGFR-2, Polyclonal Antibody (Cat# AAA71731)

Full Name

VEGFR-2 (Tyr-801)[conserved site], phospho-specific Antibody

Gene Names

KDR; FLK1; CD309; VEGFR; VEGFR2

Reactivity

Human, Rat, Mouse

Applications

Immunocytochemistry, Western Blot

Pricing

$505/0.1 mL $985/2x0.1 mL $1,440/3x0.1 mL $1,875/4x0.1 mL $2,100/5x0.1 mL

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ICC (Immunocytochemistry) (Immunocytochemical labeling of p38 MAPK in pervanadate-treated mouse C2C12. The cells were labeled with mouse monoclonal p38alpha MAPK and rabbit polyclonal p38 MAPK (Tyr-323) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.)

WB (Western Blot) (A) Western blot image of GST-recombinant p38 (K53M) mutant kinase untreated (lanes 1 & 3) or treated with Fyn kinase (lanes 2 & 4). B) Western blot analysis of p38 phosphorylation in mouse macrophages stimulated with 1mM pervanadate for 30 min. (lanes 1 & 3) then the blot was treated with alkaline phosphatase (lanes 2 & 4). Both blots were probed with anti-p38alpha (a.a. 319-328) (lanes 1 & 2) or anti-p38alpha (Tyr-323) (lanes 3 & 4).)

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p38alpha MAP Kinase, Polyclonal Antibody (Cat# AAA71695)

Full Name

p38alpha MAP Kinase (Tyr-323), phospho-specific Antibody

Gene Names

Mapk14; p38; Crk1; Mxi2; p38a; CSBP2; Csbp1; PRKM14; PRKM15; p38MAPK; p38alpha; p38-alpha

Reactivity

Human, Rat, Mouse, Chicken

Applications

Immunocytochemistry, Western Blot

Pricing

$505/0.1 mL $985/2x0.1 mL $1,440/3x0.1 mL $1,875/4x0.1 mL $2,100/5x0.1 mL

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Application Data

IHC (Immunohiostchemistry)

IHC (Immunohistochemistry)

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STAT3, Polyclonal Antibody (Cat# AAA47824)

Full Name

STAT3 (phospho-Tyr705)

Gene Names

STAT3; APRF; HIES; ADMIO; ADMIO1

Applications

Immunohistochemistry, Immunoblot

Pricing

$475/0.1 mL $925/2x0.1 mL $1,355/3x0.1 mL $1,755/4x0.1 mL $1,980/5x0.1 mL

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Application Data

IHC (Immunohistochemistry)

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Ezrin, Polyclonal Antibody (Cat# AAA47838)

Full Name

Ezrin (Phospho-Tyr353)

Gene Names

EZR; CVL; CVIL; VIL2; HEL-S-105

Applications

Immunohistochemistry, Immunoblot

Pricing

$475/0.1 mL $925/2x0.1 mL $1,355/3x0.1 mL $1,755/4x0.1 mL $1,980/5x0.1 mL

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Application Data

IHC (Immunohiostchemistry)

IF (Immunofluorescence)

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HER2, Polyclonal Antibody (Cat# AAA47848)

Full Name

HER2 (Phospho-Tyr877)

Gene Names

ERBB2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu

Applications

Immunofluorescence, Immunoblot

Pricing

$475/0.1 mL $925/2x0.1 mL $1,355/3x0.1 mL $1,755/4x0.1 mL $1,980/5x0.1 mL

WB (Western Blot) (Western blot analysis of Estrogen Receptor alpha (pY537) expression in HeLa (A), SP2/0 (B), rat spleen (C) whole cell lysates.)

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Estrogen Receptor alpha (pY537), Polyclonal Antibody (Cat# AAA105233)

Full Name

Anti-Estrogen Receptor alpha (pY537) Phospho Antibody

Gene Names

ESR1; ER; ESR; Era; ESRA; ESTRR; NR3A1

Reactivity

Human, Mouse, Rat, Bovine, Chicken, Pig

Applications

Western Blot

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

$240/0.03 mL $275/0.05 mL $345/0.1 mL $475/0.2 mL $925/2x0.2 mL $1,355/3x0.2 mL $1,755/4x0.2 mL $1,920/5x0.2 mL

Standard Curve (Sample)

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Phospho-Glycogen Synthase Kinase 3beta, ELISA Kit (Cat# AAA86566)

Full Name

Rat Phospho-Glycogen Synthase Kinase 3beta ELISA Kit

Gene Names

NPHS2; PDCN; SRN1

Reactivity

Rat

Pricing

$595/48-Strip-Wells $845/96-Strip-Wells $1,640/2x96-Strip-Wells $3,715/5x96-Strip-Wells $7,005/10x96-Strip-Wells

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using Acetyl-CoA Carboxylase (phospho-Ser80) antibody.)

WB (Western Blot) (Western blot analysis of extracts from 293 cells treated with EGF (200ng/ml, 5mins), using Acetyl-CoA Carboxylase (phospho-Ser80) antibody.)

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Acetyl-CoA Carboxylase (Phospho-Ser80), Polyclonal Antibody (Cat# AAA283676)

Full Name

Acetyl-CoA Carboxylase (Phospho-Ser80) Antibody

Gene Names

ACACA; ACC; ACAC; ACC1; ACCA; ACACAD

Reactivity

Human, mouse, rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot) (The anti-Phospho-p27Kip1-T187Pab is used in Western blot to detect Phospho-p27Kip1-T187 in HL60 tissue lysate)

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Phospho-p27Kip1 (T187), Polyclonal Antibody (Cat# AAA283718)

Full Name

Phospho-p27Kip1 (T187) Antibody

Gene Names

CDKN1B; KIP1; MEN4; CDKN4; MEN1B; P27KIP1

Reactivity

Human (Predicted Reactivity: Hamster)

Applications

Immunohistochemistry, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

DB (Dot Blot) (Dot blot analysis of Phospho-PARP1-T373 Antibody Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)

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Phospho-PARP1 (T373), Polyclonal Antibody (Cat# AAA284214)

Full Name

Phospho-PARP1 (T373) Antibody

Gene Names

PARP1; PARP; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1

Reactivity

Human

Applications

Dot Blot

Purity

This antibody is purified through a protein A column, followed by peptide affinity purification.

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot) (The anti-MET-Y1349 Pab is used in Western blot to detect MET-Y1349 in mouse kidney tissue lysate.)

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Phospho-MET (Y1349), Polyclonal Antibody (Cat# AAA284353)

Full Name

Phospho-MET (Y1349) Antibody

Gene Names

MET; HGFR; AUTS9; RCCP2; c-Met; DFNB97

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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WB (Western Blot) (Western blot analysis of extracts from cos-7 cells (Lane 2) and 3T3 cells (Lane 3), using MAP2K7 (Phospho-Thr275) Antibody. The lane on the left is treated with synthesized peptide.)

WB (Western Blot) (Western blot analysis of extracts from HeLa cells, treated with calyculinA (50ng/ml, 30mins), using MAP2K7 (Phospho-Thr275) antibody.)

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MAP2K7 (Phospho-Thr275), Polyclonal Antibody (Cat# AAA284954)

Full Name

MAP2K7 (Phospho-Thr275) Antibody

Gene Names

MAP2K7; MEK; MKK7; JNKK2; MEK 7; MAPKK7; PRKMK7; SAPKK4; SAPKK-4

Reactivity

Human, mouse, rat

Applications

Western Blot

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed MCF cells using p70 S6 Kinase(Phospho-Thr421) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p70 S6 Kinase(Phospho-Thr421) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from Jurkat cells untreated(lane 1) or treated with GM-CSF(lane 2) using p70 S6 Kinase(Phospho-Thr421) Antibody.)

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p70 S6 Kinase, Polyclonal Antibody (Cat# AAA305500)

Full Name

p70 S6 Kinase (Phospho-Thr421) Antibody

Gene Names

RPS6KB1; S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts from Hela cells, treated with UV or calf intestinal phosphatase (CIP), using HSP27 (Phospho-Ser82) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP27(Phospho-Ser82) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from HL60 cells untreated or treated with UV using HSP27(Phospho-Ser82) Antibody.)

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HSP27, Polyclonal Antibody (Cat# AAA305525)

Full Name

HSP27 (Phospho-Ser82) Antibody

Gene Names

HSPB1; CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using PLC-g1(phospho-Tyr771) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from 3T3 cells untreated or treated with serum using PLC-g1(phospho-Tyr771) Antibody.)

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PLC-gamma1, Polyclonal Antibody (Cat# AAA305526)

Full Name

PLC-gamma1 (phospho-Tyr771) Antibody

Gene Names

PLCG1; PLC1; NCKAP3; PLC-II; PLC148; PLCgamma1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using SRF (Phospho-Ser77) antibody. The picture on the right is treated with the synthesized peptide.)

WB (Western Blot) (Western blot analysis of extracts from Jurkat cells, treated with PMA (125ng/ml, 30mins), using SRF (Phospho-Ser77) antibody. The lane on the right is treated with the synthesized peptide.)

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SRF, Polyclonal Antibody (Cat# AAA305001)

Full Name

SRF (Phospho-Ser77) Antibody

Gene Names

SRF; MCM1

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATF2(Phospho-Ser62 or 44) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from MDA231 cells untreated or treated with UV using ATF2(Phospho-Ser62 or 44) Antibody.)

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ATF2, Polyclonal Antibody (Cat# AAA305029)

Full Name

ATF2 (Phospho-Ser62 or 44) Antibody

Gene Names

ATF2; HB16; CREB2; TREB7; CREB-2; CRE-BP1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using RIPK2 (Phospho-Ser176) antibody. The picture on the right is treated with the synthesized peptide.)

WB (Western Blot) (Western blot analysis of extracts from 293 cells, treated with UV (15mins), using RIPK2 (Phospho-Ser176) antibody. The lane on the right is treated with the synthesized peptide.)

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RIPK2, Polyclonal Antibody (Cat# AAA305059)

Full Name

RIPK2 (Phospho-Ser176) Antibody

Gene Names

RIPK2; CCK; RICK; RIP2; CARD3; GIG30; CARDIAK

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-Jun(Phospho-Thr93) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from Hela cells untreated or treated with PMA using c-Jun(Phospho-Thr93) Antibody.)

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c-Jun, Polyclonal Antibody (Cat# AAA305246)

Full Name

c-Jun (Phospho-Thr93) Antibody

Gene Names

JUN; AP1; AP-1; c-Jun

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts from COS7 cells (Lane 2), using C-RAF (Phospho-Ser301) Antibody. The lane on the left is treated with synthesized peptide.)

WB (Western Blot) (Western blot analysis of extracts from HuvEc, using C-RAF (Phospho-Ser301) antibody. The lane on the right is treated with the synthesized peptide.)

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C-RAF, Polyclonal Antibody (Cat# AAA305133)

Full Name

C-RAF (Phospho-Ser301) Antibody

Gene Names

RAF1; NS5; CRAF; Raf-1; c-Raf; CMD1NN

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using JunB(Phospho-Ser259) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from HT29 cells untreated or treated with EGF using JunB(Phospho-Ser259) Antibody.)

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JunB, Polyclonal Antibody (Cat# AAA305134)

Full Name

JunB (Phospho-Ser259) Antibody

Gene Names

JUNB; AP-1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using EGFR(Phospho-Ser1070) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from HUVEC cells untreated or treated with EGF using EGFR(Phospho-Ser1070) Antibody.)

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EGFR, Polyclonal Antibody (Cat# AAA305295)

Full Name

EGFR (Phospho-Ser1070) Antibody

Gene Names

EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts from Hela cells, treated with Noc or calf intestinal phosphatase (CIP), using BCL-XL (Phospho-Ser62) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from Hela cells untreated or treated with EGF using BCL-XL (Phospho-Ser62) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BCL-XL (phospho-Ser62) antibody ().)

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed HeLa cells using BCL-XL (phospho-Ser62) antibody (, Red).)

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BCL-XL, Polyclonal Antibody (Cat# AAA305618)

Full Name

BCL-XL(Phospho-Ser62) Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using cofilin(Phospho-Ser3) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cofilin(Phospho-Ser3) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from COLO205 cells using cofilin(Phospho-Ser3) Antibody (Lane 2) and the same antibody preincubated with blocking peptide(Lane1).)

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cofilin, Polyclonal Antibody (Cat# AAA305537)

Full Name

cofilin (Phospho-Ser3) Antibody

Gene Names

CFL1; CFL; cofilin; HEL-S-15

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts from Hela cells, treated with calf intestinal phosphatase (CIP), using HSP90B (Phospho-Ser254) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from Hela cells untreated(lane 1) or treated with hot shock(lane 2) using HSP90B(Phospho-Ser254) Antibody.)

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HSP90B, Polyclonal Antibody (Cat# AAA305559)

Full Name

HSP90B (Phospho-Ser254) Antibody

Gene Names

HSP90AB1; HSP84; HSPC2; HSPCB; D6S182; HSP90B

Reactivity

Human, Mouse

Applications

Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of 22RV1 HELA cells using Phospho-p16 (S326) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of JK cells using Phospho-p16 (S326) Polyclonal Antibody)

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p16, Polyclonal Antibody (Cat# AAA305589)

Full Name

p16 (Phospho-Ser326) Polyclonal Antibody

Gene Names

CDKN2A; ARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human brain tissue using Ephrin B1/B2/B3 (Phospho-Tyr324) antibody (left)or the same antibody preincubated with blocking peptide (right).)

WB (Western Blot) (Western blot analysis of extracts from K562 cells treated with serum using Ephrin B1/B2/B3 (Phospho-Tyr324) Antibody.The lane on the right is treated with the antigen-specific peptide.)

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Ephrin B1/B2/B3, Polyclonal Antibody (Cat# AAA305611)

Full Name

Ephrin B1/B2/B3 (Phospho-Tyr324) Antibody

Gene Names

EFNB1; CFND; CFNS; EFB1; EFL3; EPLG2; Elk-L; LERK2

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human brain tissue using FER (Phospho-Tyr402) antibody (left)or the same antibody preincubated with blocking peptide (right).)

WB (Western Blot) (Western blot analysis of extracts from JK cells (Lane 2) and COS7 cells (Lane 3), using FER (Phospho-Tyr402) Antibody. The lane on the left is treated with antigen-specific peptide.)

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FER, Polyclonal Antibody (Cat# AAA305385)

Full Name

FER (Phospho-Tyr402) Antibody

Gene Names

FER; TYK3; PPP1R74; p94-Fer

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using c-Jun(Phospho-Thr239) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-Jun(Phospho-Thr239) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from Hela cells untreated or treated with UV using c-Jun(Phospho-Thr239) Antibody.)

Compare (5 max)

c-Jun, Polyclonal Antibody (Cat# AAA305717)

Full Name

c-Jun (Phospho-Thr239) Antibody

Gene Names

JUN; AP1; AP-1; c-Jun

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using a-Synuclein(Phospho-Tyr125) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from C2C12 cells untreated or treated with Anisomycin using a-Synuclein(Phospho-Tyr125) Antibody.)

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alpha-Synuclein, Polyclonal Antibody (Cat# AAA305730)

Full Name

alpha-Synuclein (Phospho-Tyr125) Antibody

Gene Names

SNCA; PD1; NACP; PARK1; PARK4

Reactivity

Human

Applications

Immunofluorescence, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using NFkB-p105(Phospho-Ser927) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFkB-p105(Phospho-Ser927) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from HL60 cells untreated or treated with TNF-a using NFkB-p105(Phospho-Ser927) Antibody.)

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NFkappaB-p105/p50, Polyclonal Antibody (Cat# AAA306132)

Full Name

NFkappaB-p105/p50 (Phospho-Ser927) Antibody

Gene Names

NFKB1; p50; KBF1; p105; EBP-1; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed HeLa cells using Elk-1 (phospho-Thr417) antibody (, Red).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Elk-1 (phospho-Thr417) antibody ().)

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Elk1, Polyclonal Antibody (Cat# AAA306145)

Full Name

Elk1(Phospho-Thr417) Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts from Hela cells, treated with Noc or calf intestinal phosphatase (CIP), using APP (Phospho-Thr668) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from mouse brain tissue using APP(Phospho-668) Antibody (Lane 2) and the same antibody preincubated with blocking peptide(Lane1).)

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APP, Polyclonal Antibody (Cat# AAA306165)

Full Name

APP (Phospho-668) Antibody

Gene Names

APP; AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; CTFgamma

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human brain tissue using STK39 (Phospho-Ser309) antibody (left)or the same antibody preincubated with blocking peptide (right).)

WB (Western Blot) (Western blot analysis of extracts from COLO cells using STK39 (Phospho-Ser309) Antibody.The lane on the right is treated with the antigen-specific peptide.)

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STK39, Polyclonal Antibody (Cat# AAA305874)

Full Name

STK39 (Phospho-Ser311) Antibody

Gene Names

STK39; DCHT; PASK; SPAK

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ASK1(Phospho-Ser966) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from various cells using ASK1(Phospho-Ser966) Antibody.)

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ASK1, Polyclonal Antibody (Cat# AAA305947)

Full Name

ASK1 (Phospho-Ser966) Antibody

Gene Names

MAP3K5; ASK1; MEKK5; MAPKKK5

Reactivity

Human, Mouse, Monkey

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of COLO HELA 293T cells using Phospho-HDAC5/9 (S259/220) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of HepG2 cells using Phospho-HDAC5/9 (S259/220) Polyclonal Antibody)

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HDAC5/9, Polyclonal Antibody (Cat# AAA305817)

Full Name

HDAC5/9 (Phospho-Ser259/220) Polyclonal Antibody

Gene Names

HDAC5; HD5; NY-CO-9

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

WB (Western Blot) (Western blot analysis of 293 using p-Emt (Y512) antibody.)

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Emt, Polyclonal Antibody (Cat# AAA305994)

Full Name

Emt (Phospho-Tyr512) Polyclonal Antibody

Gene Names

ITK; EMT; LYK; LPFS1; PSCTK2

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using MARCKS(Phospho-Ser158) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from 3T3 cells using MARCKS(Phospho-Ser158) Antibody (Lane 2) and the same antibody preincubated with blocking peptide(Lane1).)

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MARCKS, Polyclonal Antibody (Cat# AAA306031)

Full Name

MARCKS (Phospho-Ser158) Antibody

Gene Names

MARCKS; MACS; 80K-L; PKCSL; PRKCSL

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

What Are Phospho Antibodies?

Protein phosphorylation is a process where a phosphate group is added to certain amino acid residues of a protein – usually serine (S), threonine (T), or tyrosine (Y) - by enzymes called kinases. This process is integral in controlling cellular signaling, cellular growth, and other biological functions.

Our catalog includes a wide range of phospho-specific antibodies that can accurately detect this important marker. They perform strongly in widely-used laboratory applications such as Western blot, flow cytometry, immunohistochemistry, and immunofluorescence microscopy. We value your trust in us and are committed to providing top-quality products and services. All of our antibodies are guaranteed to work for the applications and species indicated on our website & associated product pages.

What Are The Key Applications of Phospho Antibodies?

1. Western Blotting

One of the first steps a researcher can take in utilizing these phospho-specific antibodies, is to check if the antibody works using a technique referred to as “Western blot”. For those unfamiliar, Western Blot aids in showing whether the protein that the antibody recognizes is appearing at the correct/expected size. These phospho-specific antibodies should also be able to detect changes in the target protein’s phosphorylation (on/off state) when cells are stimulated in certain ways.

2. Staining of Fixed Cells (Immunocytochemistry)

Another routine use of these phospho-specific antibodies, is to test if the antibody is able to demonstrate similar performance when used on fixed cells (intact cells that have been preserved) as it did in the Western blot tests. It is an important aspect in many cases to confirm that the antibody works in actual intact cell samples. Ideally, the method used for cellular fixation should be the same as what is used in pathology labs (like using 10% formalin). To check if the antibody works well in tissue sections (FFPE), researchers will often test it on fixed cells that are processed similar to tissue samples.

3. Specificity Tests Using Peptides

In order to make sure that the antibody is only binding to the right target:

• Laboratory technicians will mix the antibody with phospho-peptides (short segments of the protein containing the phosphate group modification).


• If the antibody signal disappears, it is confirmation that it is binding to the correct phosphorylated location.


• A more robust test is to use both the phosphorylated and non-phosphorylated (dephosphorylated) versions of the protein. The antibody should react only with the phosphorylated one.


• Another method sometimes utilized is to treat the sample with an enzyme, such as alkaline phosphatase, that specifically removes phosphate groups. If the antibody signal disappears after this, it also confirms specificity.

4. Genetic Confirmation

As a final step, scientists can genetically manipulate the nucleotide sequence and alter the target protein by removing the exact site where phosphorylation happens. If the antibody no longer appears to detect the modified protein, it is strong evidence supporting the antibody being specific for that phosphorylated site.

Why Buy Phospho Antibodies Through Us?

• The production laboratory adheres to strict and consistent protocols prior to releasing any of these phospho-specific antibodies:


• Standard methods and proper controls in all tests to ensure high quality.


• These antibodies are tested and validated in different cell types and species.


• High quality control criterion to ensure each batch is consistent, so you will obtain reliable results every time.

FAQ

1. What Are Phospho-Specific Antibodies?

Phospho-specific antibodies are made to detect proteins only when they have a phosphate group linked to a specific amino acid residue. This empowers scientists understand if a protein is "turned on" or active, based on its phosphorylation state.

2. How to Detect Phosphorylated Proteins in a Western Blot?

To find out if a protein is phosphorylated using Western blot:

• Use a phospho-specific antibody that binds only to the phosphorylated form of the protein.


• You can also use a “regular” antibody for the same amino acid sequence of the protein that the phospho-specific antibody is binding to (but in this case, this antibody will not bind if there is a phosphate group present) in order to compare how much of it is phosphorylated versus how much is non-phosphorylated (or “total” protein, if the “normal” antibody’s epitopes are non-phospho-site-specific).

3. How to Choose the Best Antibody?

Here are some simple tips to help you pick the right antibody:

• Know your target


• Match your sample characteristics


• Confirm the intended use is appropriate


• Check “host” and “type”


• Check the “quality” of the presented data/images


• Appraise whether the available validation meets your needs