Phospho Antibodies

Phospho-specific antibodies’ typical purpose is to enable researchers to detect changes in proteins. They will exclusively bind to the amino acid sequence on a protein that has been phosphorylated (which is both a physical & chemical change) and do not bind to the same amino acid sequence on said protein if it lacks said phosphorylation. This aids in being able to clearly see and understand the data produced from this particular protein modification.

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ICC (Immunocytochemistry) (ICC staining Phospho-Histone H3 (S10) in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Phospho-Histone H3 (S10) in Ags cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Phospho-Histone H3 (S10) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Phospho-Histone H3 (S10) antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Phospho-Histone H3 (S10) on Hela cell lysates using anti-Phospho-Histone H3 (S10) antibody at 1/1, 000 dilution. Positive control: Lane 1: Untreated HeLa cell lysate Lane 2: HeLa cell lysate treated with calyculin A Lane 3: HeLa cell lysate treated with calyculin A and alkaline phosphatase)

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Histone H3, Monoclonal Antibody (Cat# AAA310994)

Full Name

Histone H3 (Phospho-S10) Antibody

Gene Names

HIST1H3A; H3/A; H3FA

Reactivity

Human, Mouse, Rat

Applications

Immunoprecipitation, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Western Blot

Purity

ProA affinity purified

Pricing

$365/0.05 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $1,990/5x0.1 mL

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FCM/FACS (Flow Cytometry) (Flow cytometric analysis of MCF-7 cells with P-V-Myb+C-Myb (S11) antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as antibody)

ICC (Immunocytochemistry) (ICC staining P-V-Myb+C-Myb (S11) in Ags cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-P-V-Myb+C-Myb (S11) antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human uterine neck tissue using anti-P-V-Myb+C-Myb (S11) antibody. Counter stained with hematoxylin.)

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P-V-Myb+C-Myb, Monoclonal Antibody (Cat# AAA310997)

Full Name

P-V-Myb+C-Myb (Phospho-S11) Antibody

Gene Names

MYB; efg; Cmyb; c-myb; c-myb_CDS

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunoprecipitation, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Western Blot

Purity

ProA affinity purified

Pricing

$365/0.05 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $1,990/5x0.1 mL

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IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-Phospho-p53 (S392) antibody. Counter stained with hematoxylin.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-Phospho-p53 (S392) antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Phospho-p53 (S392) on different lysates using anti-Phospho-p53 (S392) antibody at 1/1, 000 dilution. Positive control: Lane 1: 293 Lane 2: F9)

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p53, Monoclonal Antibody (Cat# AAA310998)

Full Name

p53 (Phospho-S392) Antibody

Gene Names

TP53; P53; BCC7; LFS1; TRP53

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunoprecipitation, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Western Blot

Purity

ProA affinity purified

Pricing

$365/0.05 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $1,990/5x0.1 mL

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ICC (Immunocytochemistry) (ICC staining Phospho-RSK1 (S380) in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Phospho-RSK1 (S380) in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Phospho-RSK1 (S380) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

WB (Western Blot) (Western blot analysis of Phospho-RSK1 (S380) on A431 cell lysates using anti-Phospho-RSK1 (S380) antibody at 1/1, 000 dilution.)

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RSK1, Monoclonal Antibody (Cat# AAA311010)

Full Name

RSK1 (Phospho-S380) Antibody

Gene Names

RPS6KA1; RSK; HU-1; RSK1; MAPKAPK1A

Reactivity

Human, Mouse, Rat

Applications

Immunoprecipitation, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Western Blot

Purity

ProA affinity purified

Pricing

$365/0.05 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $1,990/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Shc (phospho-Tyr349) antibody)

WB (Western Blot) (Western blot analysis of extracts from 293 cells treated with EGF (200ng/ml, 30mins), using Shc (phospho-Tyr349) antibody.)

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Shc (Phospho-Tyr349), Polyclonal Antibody (Cat# AAA285801)

Full Name

Shc (Phospho-Tyr349) Antibody

Gene Names

SHC1; SHC; SHCA

Reactivity

Human, mouse, rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using Chk1 (Phospho-Ser296) antibody.)

WB (Western Blot) (Western blot analysis of extracts from HUVEC cells, treated with UV (15mins), using Chk1 (Phospho-Ser296) antibody.)

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Chk1 (Phospho-Ser296), Polyclonal Antibody (Cat# AAA288551)

Full Name

Chk1 (Phospho-Ser296) Antibody

Gene Names

CHEK1; CHK1

Reactivity

Human, mouse, rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using Phospho-ERK1/2 Y222/205 Mouse mAb diluted at 1:200.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma Tissue using Phospho-ERK1/2 Y222/205 Mouse mAb diluted at 1:200)

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ERK 1/2, Monoclonal Antibody (Cat# AAA309603)

Full Name

Phospho-ERK 1/2 (Y222/205)Mouse Monoclonal Antibody (4G3)

Reactivity

Human, Rat, Mouse

Applications

Immunohistochemistry

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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DB (Dot Blot) (Dot blot analysis of anti-Phospho-CDX2 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are0.5ug per ml.)

IF (Immunofluorescence) (Confocal immunofluorescent analysis of Phospho-CDX2-S283 Antibody with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).)

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Phospho-CDX2 (S283), Polyclonal Antibody (Cat# AAA285196)

Full Name

Phospho-CDX2 (S283) Antibody

Gene Names

CDX2; CDX3; CDX-3; CDX2/AS

Reactivity

Human

Applications

Dot Blot, Immunofluorescence

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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IHC (Immunohistochemisry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot) (The anti-Phospho-CHK1-S317 Pab is used in Western blot for detection in, from left to right, Jurkat, A2058, Hela, and HL60 tissue lysates.)

DB (Dot Blot) (Dot blot analysis of anti-Phospho-CHK1-S317 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antobodies working concentration was 0.5ug per ml.)

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Phospho-CHK1 (S317), Polyclonal Antibody (Cat# AAA285457)

Full Name

Phospho-CHK1 (S317) Antibody

Gene Names

CHEK1; CHK1

Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Dot Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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IHC (Immunohistochemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot) (The anti-Phospho-CDC25A-T507 Pab is used in Western blot to detect Phospho-CDC25A-T507 in cells transfected with wild type or mutant T507 A of CDC25A. Data courtesy of Dr. Tiebang Kang of Washington University, St. Louis, MO.)

WB (Western Blot) (Western blot analysis of extracts from Hela cells, untreated or treated with calyculin A, using Phospho-Cdc25A Antibody (T507).)

IF (Immunofluorescence) (Fluorescent image of HeLa cells stained with Phospho-CDC25A(T507) Antibody. AAA285545 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)

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Phospho-CDC25A (T507), Polyclonal Antibody (Cat# AAA285545)

Full Name

Phospho-CDC25A (T507) Antibody

Gene Names

CDC25A; CDC25A2

Reactivity

Human (Predicted Reactivity: Bovine, Mouse, Rat)

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

DB (Dot Blot) (Dot blot analysis of anti-Phospho-INSR-pY1185 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

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Phospho-INSR (Y1185), Polyclonal Antibody (Cat# AAA285280)

Full Name

Phospho-INSR (Y1185) Antibody

Gene Names

INSR; HHF5; CD220

Reactivity

Human (Predicted Reactivity: Bovine, Mouse, Rat, Xenopus, Drosophila)

Applications

Dot Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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DB (Dot Blot) (Dot blot analysis of Phospho-ABL1 (Y393)/ABL2 (439) Antibody Pab (AAA285314) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot) (Western blot analysis of Phospho-ABL-Y412 Antibody Pab in A2058 cell line lysate (35ug/lane). ABL (arrow) was detected using the purified Pab.)

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Phospho-ABL (Y412), Polyclonal Antibody (Cat# AAA285314)

Full Name

Phospho-ABL (Y412) Antibody

Gene Names

ABL1; ABL; JTK7; p150; c-ABL; v-abl; c-ABL1; bcr/abl

Reactivity

Human
(Predicted Reactivity: Zebrafish, Mouse)

Applications

Immunohistochemistry, Dot Blot, Western Blot

Purity

This antibody is purified through a protein A column, followed by peptide affinity purification.

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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DB (Dot Blot) (Dot blot analysis of PDX1 Antibody (T11) Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

IF (Immunofluorescence) (Fluorescent confocal image of SY5Y cells stained with phospho-PDX1-T11 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AAA287859 phospho-PDX1-T11 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the phospho-PDX1 immunosignal mainly to the nucleus.)

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Phospho-PDX1 (T11), Polyclonal Antibody (Cat# AAA287859)

Full Name

Phospho-PDX1 (T11) Antibody

Gene Names

PDX1; GSF; IPF1; IUF1; IDX-1; MODY4; PDX-1; STF-1; PAGEN1

Reactivity

Human (Predicted Reactivity: Mouse, Rat)

Applications

Dot Blot, Immunofluorescence

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot) (The anti-Phospho-Caspase 9-S196 Pab is used in Western blot to detect Phospho-Caspase 9-S196 in Y79 cell line lysates.)

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Phospho-Caspase 9 (S196), Polyclonal Antibody (Cat# AAA287914)

Full Name

Phospho-Caspase 9 (S196) Antibody

Gene Names

CASP9; MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

WB (Western Blot) (Western blot analysis of extracts from Jurkat cells treated with TNF, using HS1 (Phospho-Tyr397) antibody.)

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HS1 (Phospho-Tyr397), Antibody (Cat# AAA287658)

Full Name

HS1 (Phospho-Tyr397) Antibody

Gene Names

HCLS1; HS1; p75; CTTNL; lckBP1

Reactivity

Human (Identities = 100%, Positives = 100%)

Applications

Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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DB (Dot Blot) (Dot blot analysis of anti-hSMAD3-S208 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

IF (Immunofluorescence) (Confocal immunofluorescent analysis of Phospho-SMAD3-S208 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).)

IF (Immunofluorescence) (Fluorescent confocal image of HeLa cells stained with phospho-SMAD3-S208 antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AAA289606 phospho-SMAD3-S208 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 uM, 25 min). Pictures were taken on a Biorevo microscope (BZ-900, Keyence).Note the highly specific localization of the phospho-SMAD3 mainly to the nucleus, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000166949).)

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Phospho-SMAD3 (S208), Polyclonal Antibody (Cat# AAA289606)

Full Name

Phospho-SMAD3 (S208) Antibody

Gene Names

SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436

Reactivity

Human (Predicted Reactivity: Chicken, Mouse, Pig, Rat)

Applications

Dot Blot, Immunofluorescence

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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WB (Western Blot) (Western blot analysis of extracts from Hela cells, treated with IL-4 or calf intestinal phosphatase (CIP), using STAT6 (Phospho-Thr645) Antibody.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT6(Phospho-Thr645) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

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STAT6, Polyclonal Antibody (Cat# AAA243060)

Full Name

Phospho-STAT6 (Thr645) Antibody

Gene Names

STAT6; STAT6B; STAT6C; D12S1644; IL-4-STAT

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Ezrin(Phospho-Tyr353) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from 293 cells untreated or treated with EGF using Ezrin(Phospho-Tyr353) Antibody.)

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EZR, Polyclonal Antibody (Cat# AAA243064)

Full Name

Phospho-EZR (Tyr353) Antibody

Gene Names

EZR; CVL; CVIL; VIL2

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BAD(Phospho-Ser112) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from cos7 cells untreated or treated with EGF using BAD(Phospho-Ser112) Antibody)

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Bad, Polyclonal Antibody (Cat# AAA243065)

Full Name

Phospho-Bad (Ser112) Antibody

Gene Names

Bad; Bbc2; AI325008

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using PKD/PKCm(Phospho-Ser910) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from EGF-treated 293 and PMA-treated HUVEC cells using PKD/PKCm(Phospho-Ser910) Antibody.)

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PRKD1, Polyclonal Antibody (Cat# AAA243076)

Full Name

Phospho-PRKD1 (Ser910) Antibody

Gene Names

PRKD1; PKD; PKCM; PRKCM; PKC-MU

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using Tau(Phospho-Ser356) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from mouse brain tissue using Tau(Phospho-Ser356) Antibody and the same antibody preincubated with blocking peptide.)

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MAPT, Polyclonal Antibody (Cat# AAA243078)

Full Name

Phospho-MAPT (Ser356) Antibody

Gene Names

MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CDK2(Phospho-Thr160) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from Hela cells untreated(lane 1) or treated with UV(lane 2) using CDK2(Phospho-Thr160) Antibody.)

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CDK2, Polyclonal Antibody (Cat# AAA243091)

Full Name

Phospho-CDK2 (Thr160) Antibody

Gene Names

CDK2; p33(CDK2)

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Stathmin 1(Phospho-Ser38) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from K562 cells untreated or treated with starvation using Stathmin 1(Phospho-Ser38) Antibody.)

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STMN1, Polyclonal Antibody (Cat# AAA243123)

Full Name

Phospho-STMN1 (Ser38) Antibody

Gene Names

STMN1; Lag; SMN; OP18; PP17; PP19; PR22; LAP18; C1orf215

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Keratin 18(Phospho-Ser33) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from HT29 cells untreated or treated with EGF using Keratin 18(Phospho-Ser33) Antibody.)

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KRT18, Polyclonal Antibody (Cat# AAA243145)

Full Name

Phospho-KRT18 (Ser33) Antibody

Gene Names

KRT18; K18; CYK18

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts from Hela cells, treated with IFNa or calf intestinal phosphatase (CIP), using HSF1 (Phospho-Ser307) Antibody.)

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using HSF1(Phospho-Ser307) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from HUVEC cells untreated(lane 1) or treated with TNF-a(lane 2) using HSF1(Phospho-Ser307) Antibody.)

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HSF1, Polyclonal Antibody (Cat# AAA243297)

Full Name

Phospho-HSF1 (Ser307) Antibody

Gene Names

HSF1; HSTF1

Reactivity

Human

Applications

Immunofluorescence, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using p53 (Phospho-Ser15) Antibody.)

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using p53(Phospho-Ser15) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p53 (Phospho-Ser15) Antibody (left) or the same antibody preincubated with blocking peptide (right).)

WB (Western Blot) (Western blot analysis of extracts from 293 cells untreated or treated with UV using p53(Phospho-Ser15) Antibody.)

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TP53, Polyclonal Antibody (Cat# AAA243358)

Full Name

Phospho-TP53 (Ser15) Antibody

Gene Names

TP53; P53; BCC7; LFS1; TRP53

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using AKT1/3 (Phospho-Tyr437/434) antibody. The picture on the right is treated with the synthesized peptide.)

WB (Western Blot) (Western blot analysis of extracts from K562 cells, treated with insulin (0.01U/ml, 15mins), using AKT1/3 (Phospho-Tyr437/434) antibody. The lane on the right is treated with the synthesized peptide.)

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AKT1/AKT3, Polyclonal Antibody (Cat# AAA243236)

Full Name

Phospho-AKT1/AKT3 (Tyr437/434) Antibody

Gene Names

AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts from 293 cells, treated with serum or calf intestinal phosphatase (CIP), using Akt2 (Phospho-Ser474) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Akt2(Phospho-Ser474) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from 293 cells untreated(lane 1) or treated with serum(lane 2) using Akt2(Phospho-Ser474) Antibody.)

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AKT2, Polyclonal Antibody (Cat# AAA243279)

Full Name

Phospho-AKT2 (Ser474) Antibody

Gene Names

AKT2; PKBB; PRKBB; HIHGHH; PKBBETA; RAC-BETA

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using Rb(Phospho-Ser795) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rb(Phospho-Ser795) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from K562 cells untreated(lane 1) or treated with serum(lane 2) using Rb(Phospho-Ser795) Antibody.)

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RB1, Polyclonal Antibody (Cat# AAA243283)

Full Name

Phospho-RB1 (Ser795) Antibody

Gene Names

RB1; RB; pRb; OSRC; pp110; p105-Rb

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts from HL60 cells, treated with calf intestinal phosphatase (CIP), using Zap-70 (Phospho-Tyr319) Antibody.)

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using Zap-70(Phospho-Tyr319) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from Jurkat cells using Zap-70(Phospho-Tyr319) Antibody(Lane 2) and the same antibody preincubated with blocking peptide(Lane1).)

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ZAP70, Polyclonal Antibody (Cat# AAA243291)

Full Name

Phospho-ZAP70 (Tyr319) Antibody

Gene Names

ZAP70; SRK; STD; TZK; STCD; ZAP-70

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts from 293 cells, treated with calf intestinal phosphatase (CIP), using CREB(Phospho-Ser142) Antibody.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CREB (Phospho-Ser142) Antibody.)

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CREB1, Polyclonal Antibody (Cat# AAA243179)

Full Name

Phospho-CREB1 (Ser142) Antibody

Gene Names

CREB1; CREB

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human placenta tissue using PAK1/2 (Phospho-Ser199) antibody (left)or the same antibody preincubated with blocking peptide (right).)

WB (Western Blot) (Western blot analysis of extracts from LOVO cells treated with starved using PAK1/2 (Phospho-Ser199) Antibody.The lane on the right is treated with the antigen-specific peptide.)

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PAK1, Polyclonal Antibody (Cat# AAA243200)

Full Name

Phospho-PAK1 (Ser199) Antibody

Gene Names

PAK1; PAKalpha

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human brain tissue using IGF2R (Phospho-Ser2409) antibody (left)or the same antibody preincubated with blocking peptide (right).)

WB (Western Blot) (Western blot analysis of extracts from COS-7 cells treated with UV using IGF2R (Phospho-Ser2409) Antibody.The lane on the right is treated with the antigen-specific peptide.)

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IGF2R, Polyclonal Antibody (Cat# AAA243201)

Full Name

Phospho-IGF2R (Ser2409) Antibody

Gene Names

IGF2R; MPR1; MPRI; CD222; CIMPR; M6P-R

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic staining using GSK3β (Phospho-Ser9) Antibody.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human Lung carcinoma tissue using GSK3β (Phospho-Ser9) Antibody.)

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic staining using GSK3β(Phospho-Ser9) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GSK3β (Phospho-Ser9) Antibody (left) or the same antibody preincubated with blocking peptide (right).)

WB (Western Blot) (Western blot analysis of extracts from 293 cells untreated(lane 1) or treated with serum(lane 2) using GSK3β(Phospho-Ser9) Antibody.)

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GSK3B, Polyclonal Antibody (Cat# AAA243382)

Full Name

Phospho-GSK3B (Ser9) Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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WB (Western Blot) (Detection of human Phospho BCKDHA (S292) by western blot. Samples: Whole cell lysate (50 ug) from HEK293T cells mock treated (-) or treated with phosphatases (+). Antibody: Rabbit anti-Phospho BCKDHA (S292) recombinant monoclonal antibody (AAA213555 lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)

WB (Western Blot) (Detection of human Phospho BCKDHA (S292) by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, GaMG, Hep-G2, HeLa, and LNCaP cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho-BCKDHA-S292 recombinant monoclonal antibody (AAA213555 lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)

WB (Western Blot) (Detection of mouse Phospho BCKDHA (S292) by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, CT26, TCMK-1, BW5147.3, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho BCKDHA (S292) recombinant monoclonal antibody (AAA213555 lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)

Simple Western (Detection of mouse pBCKDHA (S292) by Simple Western. Samples: Whole cell lysate (0.4 mg/mL) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho BCKDHA (S292) recombinant monoclonal antibody (AAA213555) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram.)

Simple Western (Detection of human pBCKDHA (S292) by Simple Western. Samples: Whole cell lysate (0.08 mg/mL) from HEK293T cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho BCKDHA (S292) recombinant monoclonal antibody (AAA213555) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram.)

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BCKDHA, Monoclonal Recombinant Antibody (Cat# AAA213555)

Full Name

Rabbit anti-Phospho BCKDHA (S292) Recombinant Monoclonal Antibody [BLR060F]

Gene Names

BCKDHA; MSU; MSUD1; OVD1A; BCKDE1A

Reactivity

Human, Mouse

Applications

Simple Western Blot, Western Blot

Purity

Purified

Pricing

$265/0.02 mL $455/0.1 mL $890/2x0.1 mL $955/5x0.02 mL $1,295/3x0.1 mL $1,680/4x0.1 mL $1,825/5x0.1 mL

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IHC (Immunohiostchemistry) (IHC analyses of human HCC samples were performed with the indicated antibodies in the presence or absence of a PCK1 pS90 blocking peptide.)

Application Data (Huh7 cells expressing DYKDDDDK-PCK1 were treated with or without IGF1 (100 ng/ml) for 1 h. Immunoprecipitation and immunoblotting analyses were performed with the indicated antibodies in the presence or absence of a PCK1 pS90 blocking peptide.)

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PCK1, Polyclonal Antibody (Cat# AAA312985)

Full Name

PCK1 (Phospho-Ser90) Antibody

Gene Names

PCK1; PEPCK1; PEPCKC; PEPCK-C

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purifiedfrom rabbitantiserum by affinity-chromatography using epitope-specific phosphopeptide.The antibody againstnon-phosphopeptidewas removed by chromatographyusing non-phosphopeptidecorresponding tothephosphorylationsite.

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,555/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of 5-Lipoxygenase (phospho-Ser272) staining in human skeletal muscle formalin fixed paraffin embedded tissue section. The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot) (Western blot analysis of 5-Lipoxygenase (phospho-Ser272) expression in HuvEc whole cell lysates.)

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5-Lipoxygenase (phospho-Ser272), Polyclonal Antibody (Cat# AAA310499)

Full Name

5-Lipoxygenase (phospho-Ser272) Antibody

Gene Names

ALOX5; 5-LO; 5LPG; LOG5; 5-LOX

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Immunogen affinity chromatography

Pricing

$340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of Ah Receptor (phospho-Ser36) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot) (Western blot analysis of Ah Receptor (phospho-Ser36) expression in A431, HepG2, NIH3T3 whole cell lysates.)

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Ah Receptor (phospho-Ser36), Polyclonal Antibody (Cat# AAA310508)

Full Name

Ah Receptor (phospho-Ser36) Antibody

Gene Names

AHR; bHLHe76

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Immunogen affinity chromatography

Pricing

$340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of PRKD1/2/3 (phospho-Ser738/Ser742) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot) (Western blot analysis of PRKD1/2/3 (phospho-Ser738/S742) expression in HT29, rat astrocytes whole cell lysates.)

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PRKD1/2/3 (phospho-Ser738/Ser742), Polyclonal Antibody (Cat# AAA310511)

Full Name

PRKD1/2/3 (phospho-Ser738/Ser742) Antibody

Gene Names

PRKD1; PKD; PKCM; CHDED; PRKCM; PKC-MU

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Immunogen affinity chromatography

Pricing

$340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescent analysis of HSP20 (phospho-Ser16) staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of HSP20 (phospho-Ser16) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot) (Western blot analysis of HSP20 (phospho-Ser16) expression in MCF7, HEK293, NIH3T3, H9C2 whole cell lysates.)

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HSP20 (phospho-Ser16), Polyclonal Antibody (Cat# AAA310518)

Full Name

HSP20 (phospho-Ser16) Antibody

Gene Names

HSPB6; HEL55; Hsp20; PPP1R91

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Immunogen affinity chromatography

Pricing

$340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescent analysis of ANAPC1 (phospho-Ser688) staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of ANAPC1 (phospho-Ser688) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot) (Western blot analysis of ANAPC1 (phospho-Ser688) expression in HEK293 LPS-treated whole cell lysates.)

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ANAPC1 (phospho-Ser688), Polyclonal Antibody (Cat# AAA310519)

Full Name

ANAPC1 (phospho-Ser688) Antibody

Gene Names

ANAPC1; APC1; MCPR; TSG24

Reactivity

Human, Mouse

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Immunogen affinity chromatography

Pricing

$340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using p90 RSK (Phospho-Thr573) antibody.)

WB (Western Blot) (Western blot analysis of extracts from 293 cells, treated with UV (30mins), using p90 RSK (Phospho-Thr573) antibody.)

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p90 RSK (Phospho-Thr573), Polyclonal Antibody (Cat# AAA287402)

Full Name

p90 RSK (Phospho-Thr573) Antibody

Gene Names

RPS6KA1; RSK; HU-1; RSK1; p90Rsk; MAPKAPK1A

Reactivity

Human, mouse, rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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IHC (Immunohistochemisry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot) (The anti-Phospho-Rad9-S328 Pab is used in Western blot to detect Phospho-Rad9-S328 in HL60 (left) and Y79 (right) tissue lysates.)

DB (Dot Blot) (Dot blot analysis of anti-Phospho-Rad9-S328 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

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Phospho-RAD9 (S328), Polyclonal Antibody (Cat# AAA288986)

Full Name

Phospho-RAD9 (S328) Antibody

Gene Names

RAD9A; RAD9

Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Dot Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer, using Phospho-eIF2 alpha (Ser51) Antibody.)

WB (Western Blot) (Western blot analysis of Phospho-eIF2 alpha (Ser51) expression in (1)HeLa cell lysates treated with Calyculin A;(2) Untreated HeLa cell lysates.)

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eIF2 alpha, Monoclonal Recombinant Antibody (Cat# AAA314838)

Full Name

eIF2 alpha (Phospho-Ser51) Rabbit mAb

Gene Names

EIF2S1; EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Affinity-chromatography

Pricing

$290/0.05 mL $365/0.1 mL $705/2x0.1 mL $1,030/3x0.1 mL $1,335/4x0.1 mL $1,425/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescent analysis of HeLa cells treated with Calyculin A, using Phospho-GSK3 beta (Ser9) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human kidney, using Phospho-GSK3 beta (Ser9) Antibody.)

WB (Western Blot) (Western blot analysis of GSK3 beta (phospho S9) expression in 293T cell lysates, treated with Calyculin A.)

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GSK3beta, Monoclonal Recombinant Antibody (Cat# AAA314872)

Full Name

GSK3beta (Phospho-Ser9) Rabbit mAb

Reactivity

Human

Applications

Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Affinity-chromatography

Pricing

$290/0.05 mL $365/0.1 mL $705/2x0.1 mL $1,030/3x0.1 mL $1,335/4x0.1 mL $1,425/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from NIH/3T3 cells treated with EGF 200ng/ml 5', using MER/SKY (Phospho-Tyr749/681) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using MER/SKY (Phospho-Tyr749/681) Antibody. The picture on the right is blocked with the phospho peptide.)

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MER/SKY, Polyclonal Antibody (Cat# AAA316617)

Full Name

MER/SKY (Phospho-Tyr749/681) Antibody

Gene Names

MERTK; MER; RP38; c-Eyk; c-mer; Tyro12

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using GATA1(Phospho-Ser142) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GATA1(Phospho-Ser142) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from HL60 cells untreated or treated with PMA using GATA1(Phospho-Ser142) Antibody.)

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GATA1, Polyclonal Antibody (Cat# AAA302543)

Full Name

GATA1 (Phospho-Ser142) Antibody

Gene Names

GATA1; GF1; GF-1; NFE1; XLTT; ERYF1; NF-E1; XLANP; XLTDA; GATA-1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using CDK6(phospho-Tyr24) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using CDK6(Phospho-Tyr24) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from 293 cells untreated(lane 1) or treated with starvation(lane 2) using CDK6(phospho-Tyr24) Antibody.)

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CDK6, Polyclonal Antibody (Cat# AAA302915)

Full Name

CDK6 (phospho-Tyr24) Antibody

Gene Names

CDK6; MCPH12; PLSTIRE

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using Androgen Receptor(Phospho-Ser213) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from DU145 cells using Androgen Receptor(Phospho-Ser213) Antibody (Lane 2) and the same antibody preincubated with blocking peptide(Lane1).)

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Androgen Receptor, Polyclonal Antibody (Cat# AAA302949)

Full Name

Androgen Receptor (Phospho-Ser213) Antibody

Gene Names

AR; KD; AIS; AR8; TFM; DHTR; SBMA; HYSP1; NR3C4; SMAX1; HUMARA

Reactivity

Human

Applications

Immunofluorescence, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts from JK cells (Lane 2), using ALOX5 (Phospho-Ser523) Antibody. The lane on the left is treated with synthesized peptide.)

WB (Western Blot) (Western blot analysis of extracts from Jurkat cells, using ALOX5 (Phospho-Ser523) antibody. The lane on the right is treated with the synthesized peptide.)

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ALOX5, Polyclonal Antibody (Cat# AAA302989)

Full Name

ALOX5 (Phospho-Ser523) Antibody

Gene Names

ALOX5; 5-LO; 5LPG; LOG5; 5-LOX

Applications

Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

What Are Phospho Antibodies?

Protein phosphorylation is a process where a phosphate group is added to certain amino acid residues of a protein – usually serine (S), threonine (T), or tyrosine (Y) - by enzymes called kinases. This process is integral in controlling cellular signaling, cellular growth, and other biological functions.

Our catalog includes a wide range of phospho-specific antibodies that can accurately detect this important marker. They perform strongly in widely-used laboratory applications such as Western blot, flow cytometry, immunohistochemistry, and immunofluorescence microscopy. We value your trust in us and are committed to providing top-quality products and services. All of our antibodies are guaranteed to work for the applications and species indicated on our website & associated product pages.

What Are The Key Applications of Phospho Antibodies?

1. Western Blotting

One of the first steps a researcher can take in utilizing these phospho-specific antibodies, is to check if the antibody works using a technique referred to as “Western blot”. For those unfamiliar, Western Blot aids in showing whether the protein that the antibody recognizes is appearing at the correct/expected size. These phospho-specific antibodies should also be able to detect changes in the target protein’s phosphorylation (on/off state) when cells are stimulated in certain ways.

2. Staining of Fixed Cells (Immunocytochemistry)

Another routine use of these phospho-specific antibodies, is to test if the antibody is able to demonstrate similar performance when used on fixed cells (intact cells that have been preserved) as it did in the Western blot tests. It is an important aspect in many cases to confirm that the antibody works in actual intact cell samples. Ideally, the method used for cellular fixation should be the same as what is used in pathology labs (like using 10% formalin). To check if the antibody works well in tissue sections (FFPE), researchers will often test it on fixed cells that are processed similar to tissue samples.

3. Specificity Tests Using Peptides

In order to make sure that the antibody is only binding to the right target:

• Laboratory technicians will mix the antibody with phospho-peptides (short segments of the protein containing the phosphate group modification).


• If the antibody signal disappears, it is confirmation that it is binding to the correct phosphorylated location.


• A more robust test is to use both the phosphorylated and non-phosphorylated (dephosphorylated) versions of the protein. The antibody should react only with the phosphorylated one.


• Another method sometimes utilized is to treat the sample with an enzyme, such as alkaline phosphatase, that specifically removes phosphate groups. If the antibody signal disappears after this, it also confirms specificity.

4. Genetic Confirmation

As a final step, scientists can genetically manipulate the nucleotide sequence and alter the target protein by removing the exact site where phosphorylation happens. If the antibody no longer appears to detect the modified protein, it is strong evidence supporting the antibody being specific for that phosphorylated site.

Why Buy Phospho Antibodies Through Us?

• The production laboratory adheres to strict and consistent protocols prior to releasing any of these phospho-specific antibodies:


• Standard methods and proper controls in all tests to ensure high quality.


• These antibodies are tested and validated in different cell types and species.


• High quality control criterion to ensure each batch is consistent, so you will obtain reliable results every time.

FAQ

1. What Are Phospho-Specific Antibodies?

Phospho-specific antibodies are made to detect proteins only when they have a phosphate group linked to a specific amino acid residue. This empowers scientists understand if a protein is "turned on" or active, based on its phosphorylation state.

2. How to Detect Phosphorylated Proteins in a Western Blot?

To find out if a protein is phosphorylated using Western blot:

• Use a phospho-specific antibody that binds only to the phosphorylated form of the protein.


• You can also use a “regular” antibody for the same amino acid sequence of the protein that the phospho-specific antibody is binding to (but in this case, this antibody will not bind if there is a phosphate group present) in order to compare how much of it is phosphorylated versus how much is non-phosphorylated (or “total” protein, if the “normal” antibody’s epitopes are non-phospho-site-specific).

3. How to Choose the Best Antibody?

Here are some simple tips to help you pick the right antibody:

• Know your target


• Match your sample characteristics


• Confirm the intended use is appropriate


• Check “host” and “type”


• Check the “quality” of the presented data/images


• Appraise whether the available validation meets your needs