Phospho Antibodies

Phospho-specific antibodies’ typical purpose is to enable researchers to detect changes in proteins. They will exclusively bind to the amino acid sequence on a protein that has been phosphorylated (which is both a physical & chemical change) and do not bind to the same amino acid sequence on said protein if it lacks said phosphorylation. This aids in being able to clearly see and understand the data produced from this particular protein modification.

Standard Curve (Sample)

Compare (5 max)

Phospho protein kinase C, ELISA Kit (Cat# AAA87832)

Full Name

Human Phospho protein kinase C ELISA Kit

Reactivity

Human

Pricing

$595/48-Strip-Wells $845/96-Strip-Wells $1,640/2x96-Strip-Wells $3,715/5x96-Strip-Wells $7,005/10x96-Strip-Wells

Standard Curve (Sample)

Compare (5 max)

Phospho-Glycogen Synthase Kinase 3, ELISA Kit (Cat# AAA84904)

Full Name

Rat Phospho-Glycogen Synthase Kinase 3 ELISA Kit

Reactivity

Rat

Pricing

$595/48-Strip-Wells $845/96-Strip-Wells $1,640/2x96-Strip-Wells $3,715/5x96-Strip-Wells $7,005/10x96-Strip-Wells

Standard Curve (Sample)

Compare (5 max)

Phospho Nuclear Factor kappa B, ELISA Kit (Cat# AAA83876)

Full Name

Human Phospho Nuclear Factor kappa B ELISA Kit

Reactivity

Human

Pricing

$595/48-Strip-Wells-(Competitive) $595/48-Strip-Wells-(Sandwich) $845/96-Strip-Wells-(Sandwich) $845/96-Strip-Wells-(Competitive) $1,640/2x96-Strip-Wells-(Competitive) $3,715/5x96-Strip-Wells-(Competitive) $3,715/5x96-Strip-Wells-(Sandwich) $7,005/10x96-Strip-Wells-(Competitive) $7,005/10x96-Strip-Wells-(Sandwich)

Standard Curve (Sample)

Compare (5 max)

Phospho protein kinase C, ELISA Kit (Cat# AAA86455)

Full Name

Mouse Phospho protein kinase C ELISA Kit

Reactivity

Mouse

Pricing

$595/48-Strip-Wells $845/96-Strip-Wells $1,640/2x96-Strip-Wells $3,715/5x96-Strip-Wells $7,005/10x96-Strip-Wells

< >

<< Previous Next >>

IHC (Immunohistochemisry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot) (The anti-Phospho-Bad-S134 Pab is used in Western blot to detect Phospho-Bad-S134 in HL60 tissue lysate)

IHC (Immunohistochemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Compare (5 max)

Phospho-Bad (S134), Polyclonal Antibody (Cat# AAA284121)

Full Name

Phospho-Bad (S134) Antibody

Gene Names

BAD; BBC2; BCL2L8

Reactivity

Human (Predicted Reactivity: Mouse, Rat)

Applications

Western Blot, Immunohistochemistry

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

DB (Dot Blot) (Dot blot analysis of Phospho-PARP1-S177 Antibody Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)

Compare (5 max)

Phospho-PARP1 (S177), Polyclonal Antibody (Cat# AAA284168)

Full Name

Phospho-PARP1 (S177) Antibody

Gene Names

PARP1; PARP; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1

Reactivity

Human (Predicted Reactivity: Hamster, Mouse, Rat)

Applications

Dot Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot) (The anti-Phospho-p16-INK4A-S140 Pab is used in Western blot to detect Phospho-p16-INK4A-S140 in A2058 tissue lysate)

Compare (5 max)

Phospho-p16-INK4A (S140), Polyclonal Antibody (Cat# AAA284679)

Full Name

Phospho-p16-INK4A (S140) Antibody

Gene Names

CDKN2A; ARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

< >

<< Previous Next >>

DB (Dot Blot) (Dot blot analysis of anti-Phospho-KLF4-S245 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

IF (Immunofluorescence) (Fluorescent confocal image of HeLa cells stained with AAA284718 Phospho-KLF4-S245 antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with AAA284718 Phospho-KLF4-S245 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 uM, 25 min). Note the highly specific localization of the phosphorylated KLF4 immunoreactivity to the nuclei but not the nucleoli or the cytoplasm.)

Compare (5 max)

Phospho-KLF4 (S245), Polyclonal Antibody (Cat# AAA284718)

Full Name

Phospho-KLF4 (S245) Antibody

Gene Names

KLF4; EZF; GKLF

Reactivity

Human (Predicted Reactivity: Mouse)

Applications

Immunofluorescence, Dot Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

DB (Dot Blot) (Dot blot analysis of MUC1 Antibody (AAA284599) Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

Compare (5 max)

Phospho-MUC1 (Y1229), Polyclonal Antibody (Cat# AAA284599)

Full Name

Phospho-MUC1 (Y1229) Antibody

Gene Names

MUC1; EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; CD227; H23AG; MCKD1; MUC-1; ADMCKD; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC

Reactivity

Human

Applications

Dot Blot

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human heart tissue, using Smad1 (phospho-Ser465) antibody.)

WB (Western Blot) (Western blot analysis of extracts from HeLa cells treated with Serum (10%, 15min), using Smad1 (phospho-Ser465) antibody.)

Compare (5 max)

Smad1 (Phospho-Ser465), Polyclonal Antibody (Cat# AAA285118)

Full Name

Smad1 (Phospho-Ser465) Antibody

Gene Names

SMAD1; BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1

Reactivity

Human, mouse, rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

< >

<< Previous Next >>

Application Data

Application Data

Compare (5 max)

HDAC3 Phospho Ser424, Polyclonal Antibody (Cat# AAA60353)

Full Name

HDAC3 Phospho Ser424 antibody (pAb)

Gene Names

HDAC3; HD3; RPD3; RPD3-2

Reactivity

Human, Mouse

Applications

Western Blot, Dot Blot

Purity

Protein A Chromatography

Pricing

$250/0.01 mg $440/0.05 mg $705/0.1 mg $1,375/2x0.1 mg $2,005/3x0.1 mg $2,605/4x0.1 mg $3,015/5x0.1 mg

< >

<< Previous Next >>

ICC (Immunocytochemistry) (Immunocytochemical labeling of phosphorylated MLC in paraformaldehyde fixed A7r5 cells. The cells were dual-labeled with anti-MLC (MM3441; middle) and anti-MLC (MP4201; top left), anti-MLC (Ser-19) (MP4221; middle left) and anti-MLC (Ser-1) (MP3461; bottom left). Goat anti-Mouse DyLight 488 and Goat anti-Rabbit DyLight 594 were used for detection of primary antibodies. The overlay of staining patterns are shown to the right.)

WB (Western Blot) (Western blot analysis of C2C12 cells untreated (lanes 1, 3, 5, & 7) or treated with Lambda phosphatase (lanes 2, 4, 6, & 8). The blots were probed with monoclonal anti-MLC20 (clone MY-21) (lanes 1 & 2), polyclonal anti-MLC (Ser-19) phosho-specific (lanes 3 & 4), anti-MLC (Ser-1) phosho-specific (lanes 5 & 6), or anti-MLC (a.a. 11-22) (lanes 7 & 8).)

Compare (5 max)

Myosin, Polyclonal Antibody (Cat# AAA71664)

Full Name

Myosin Light Chain (Ser-19), phospho-specific Antibody

Gene Names

MYL12A; MLCB; MRCL3; MRLC3; MYL2B; MLC-2B; HEL-S-24

Reactivity

Human, Rat, Mouse, Chicken

Applications

Immunocytochemistry, Western Blot

Pricing

$505/0.1 mL $985/2x0.1 mL $1,440/3x0.1 mL $1,875/4x0.1 mL $2,100/5x0.1 mL

< >

<< Previous Next >>

ICC (Immunocytochemistry) (Immunocytochemical labeling of VAV2 in control and pervanadate-treated human A431 cells. The cells were fixed in paraformaldehyde and permeabilized using NP-40. Then labeled with rabbit polyclonal Vav2 (Tyr-142). The antibody was detected using goat anti-rabbit DyLight 594.)

WB (Western Blot) (Western blot of human A431 cells treated with EGF (lanes 1, 3, & 5) then the blot was exposed to alkaline phosphatase (lanes 2, 4, & 6). The blots were probed with anti-Vav (a.a. 165-174) (lanes 1 & 2), anti-Vav2 (Tyr-142) (lanes 3 & 4), or anti-Vav2 (Tyr-172) (lanes 5 & 6).)

Compare (5 max)

Vav2, Polyclonal Antibody (Cat# AAA71727)

Full Name

Vav2 (Tyr-142)[conserved site], phospho-specific Antibody

Gene Names

Vav2; Vav-2; AI847175; 2810040F13Rik

Reactivity

Human, Rat, Mouse, Chicken

Applications

Immunocytochemistry, Western Blot

Pricing

$505/0.1 mL $985/2x0.1 mL $1,440/3x0.1 mL $1,875/4x0.1 mL $2,100/5x0.1 mL

< >

<< Previous Next >>

ICC (Immunocytochemistry) (Immunocytochemical labeling of VASP phosphorylation in rabbit spleen fibroblasts treated with Calyculin A. The cells were labeled with rabbit polyclonal VASP (Thr-278) antibody, then detected using appropriate secondary antibodies conjugated to Cy3. The antibody was used in the absence (top left) or presence (top right) of blocking peptide (VX2785). Corresponding phase images are shown bottom left and right.)

WB (Western Blot) (Western blot image of human A431 cells stimulated with calyculin A (100nM) for 30 min. The blots were untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4), then probed with mouse monoclonal VASP (C-term.) antibody (lanes 1 & 2) or rabbit polyclonal VASP (Thr-278) phospho-specific antibody (lanes 3 & 4).)

Compare (5 max)

VASP, Polyclonal Antibody (Cat# AAA71729)

Full Name

VASP (Thr-278), phospho-specific Antibody

Reactivity

Human, Rat, Mouse

Applications

Immunocytochemistry, Western Blot

Pricing

$505/0.1 mL $985/2x0.1 mL $1,440/3x0.1 mL $1,875/4x0.1 mL $2,100/5x0.1 mL

Application Data

Compare (5 max)

c-Cbl (Tyr-700), Monoclonal Antibody (Cat# AAA71501)

Full Name

Anti-c-Cbl (Tyr-700), phospho-specific

Gene Names

CBL; CBL2; NSLL; C-CBL; RNF55

Reactivity

Human, Rat, Mouse

Applications

Western Blot

Purity

Protein A Purified

Pricing

$505/0.1 mL $985/2x0.1 mL $1,440/3x0.1 mL $1,875/4x0.1 mL $2,100/5x0.1 mL

Application Data

Compare (5 max)

Paxillin (Tyr-31), Monoclonal Antibody (Cat# AAA71507)

Full Name

Anti-Paxillin (Tyr-31), phospho-specific

Gene Names

Pxn; Pxn

Reactivity

Human, Rat, Mouse

Applications

Western Blot

Purity

Protein A Purified

Pricing

$505/0.1 mL $985/2x0.1 mL $1,440/3x0.1 mL $1,875/4x0.1 mL $2,100/5x0.1 mL

< >

<< Previous Next >>

Application Data

Application Data

Compare (5 max)

EGFR (Ser-1142), Polyclonal Antibody (Cat# AAA71519)

Full Name

EGFR (Ser-1142), phospho-specific

Gene Names

EGFR; ERBB; HER1; mENA; ERBB1; PIG61

Reactivity

Human, Rat, Mouse

Applications

Immunocytochemistry, Western Blot

Purity

Affinity Purified

Pricing

$505/0.1 mL $985/2x0.1 mL $1,440/3x0.1 mL $1,875/4x0.1 mL $2,100/5x0.1 mL

Application Data

Compare (5 max)

Integrin b4 (Tyr-1494), Polyclonal Antibody (Cat# AAA71523)

Full Name

Integrin beta4 (Tyr-1494), phospho-specific

Reactivity

Human, Rat, Mouse

Applications

Western Blot

Purity

Affinity Purified

Pricing

$505/0.1 mL $985/2x0.1 mL $1,440/3x0.1 mL $1,875/4x0.1 mL $2,100/5x0.1 mL

Application Data (Western Blot: The whole cell lysate derived from EGF stimulated A431 immunoblotted by Rabbit anti AMPK alpha 1 (pT172) antibody (Cat#AAA71372) at 1:500 (lane 2).BSA was loaded as a negative control (Lane 3).Observed a major immunoreactive band at molecular weight ~63kDa.)

Compare (5 max)

AMPK-alpha (pT172), Antibody (Cat# AAA71372)

Full Name

Rabbit anti Phospho- AMPK alpha 1 (pThr 172)

Reactivity

Human, Rat, Mouse, Bovine, Dog, Chicken, Dog, Insect

Applications

ELISA

Purity

The Rabbit IgG is purified by site-modified Epitope Affinity Purification.

Pricing

$365/0.1 mg $705/2x0.1 mg $1,030/3x0.1 mg $1,335/4x0.1 mg $1,420/5x0.1 mg

< >

<< Previous Next >>

WB (Western Blot) (Western Blot analysis of HepG2+PMA cells using Phospho-Neu (Y1248) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of 293 cells using Phospho-Neu (Y1248) Polyclonal Antibody)

Compare (5 max)

Neu, Polyclonal Antibody (Cat# AAA303699)

Full Name

Neu (Phospho-Tyr1248) Polyclonal Antibody

Gene Names

ERBB2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Myc(Phospho-Ser373) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from A431 cells untreated or treated with EGF using Myc(Phospho-Ser373) Antibody.)

Compare (5 max)

MYC, Polyclonal Antibody (Cat# AAA243052)

Full Name

Phospho-MYC (Ser373) Antibody

Gene Names

MYC; MRTL; c-Myc; bHLHe39

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT5A(Phospho-Tyr694) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from K562 cells untreated or treated with G-CSF using STAT5A(Phospho-Tyr694) Antibody.)

Compare (5 max)

STAT5A, Polyclonal Antibody (Cat# AAA243057)

Full Name

Phospho-STAT5A (Tyr694) Antibody

Gene Names

STAT5A; MGF; STAT5

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using Shc1(Phospho-Tyr427) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Shc1(Phospho-Tyr427) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from 293 cells untreated or treated with PMA using Shc1(Phospho-Tyr427) Antibody.)

Compare (5 max)

SHC1, Polyclonal Antibody (Cat# AAA243320)

Full Name

Phospho-SHC1 (Tyr427) Antibody

Gene Names

SHC1; SHC; SHCA

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using NF-κB p100 (phospho- ser866) antibody.)

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed HeLa cells using NF-kappa;B p100 (phospho-ser866) antibody.)

WB (Western Blot) (Western blot analysis of extracts from ovary cancer cells using NF-κB p100 (phospho-Ser866) antibody.)

Compare (5 max)

NFKB2, Polyclonal Antibody (Cat# AAA243336)

Full Name

Phospho-NFKB2 (Ser866) Antibody

Gene Names

NFKB2; p52; p105; H2TF1; LYT10; LYT-10; NF-kB2

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using RIPK2 (Phospho-Ser176) antibody. The picture on the right is treated with the synthesized peptide.)

WB (Western Blot) (Western blot analysis of extracts from 293 cells, treated with UV (15mins), using RIPK2 (Phospho-Ser176) antibody. The lane on the right is treated with the synthesized peptide.)

Compare (5 max)

RIPK2, Polyclonal Antibody (Cat# AAA243238)

Full Name

Phospho-RIPK2 (Ser176) Antibody

Gene Names

RIPK2; CCK; RICK; RIP2; CARD3; GIG30; CARDIAK

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed MCF7 cells using VEGFR2(Phospho-Tyr951) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using VEGFR2(Phospho-Tyr951) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from G-CSF-treated HUVEC and serum-treated HT29 cells using VEGFR2(Phospho-Tyr951) Antibody.)

Compare (5 max)

KDR, Polyclonal Antibody (Cat# AAA243272)

Full Name

Phospho-KDR (Tyr951) Antibody

Gene Names

KDR; FLK1; CD309; VEGFR; VEGFR2

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human brain tissue using FER (Phospho-Tyr402) antibody (left)or the same antibody preincubated with blocking peptide (right).)

Compare (5 max)

FER, Polyclonal Antibody (Cat# AAA243206)

Full Name

Phospho-FER (Tyr402) Antibody

Gene Names

FER; FerT; TYK3; PPP1R74

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human brain tissue using p47 phox (Phospho-Ser345) antibody (left)or the same antibody preincubated with blocking peptide (right).)

WB (Western Blot) (Western blot analysis of extracts from HepG2 cells treated with TNF using p47 phox (Phospho-Ser345) Antibody.The lane on the right is treated with the antigen-specific peptide.)

Compare (5 max)

NCF1, Polyclonal Antibody (Cat# AAA243227)

Full Name

Phospho-NCF1 (Ser345) Antibody

Gene Names

NCF1; NCF1A; NOXO2; p47phox; SH3PXD1A

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of extracts from COS7 cells, treated with starve or calf intestinal phosphatase (CIP), using Estrogen Receptor-alpha (Phospho-Ser118) Antibody.)

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed MCF cells using Estrogen Receptor-a(Phospho-Ser118) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Estrogen Receptor-a(Phospho-Ser118) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from MDA cells untreated or treated with Estradiol using Estrogen Receptor-a(Phospho-Ser118) Antibody.)

Compare (5 max)

Estrogen Receptor-alpha, Polyclonal Antibody (Cat# AAA305386)

Full Name

Estrogen Receptor-alpha (Phospho-Ser118) Antibody

Gene Names

ESR1; ER; ESR; Era; ESRA; ESTRR; NR3A1

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p62Dok(Phospho-Tyr398) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from K562 cells untreated or treated with H2O2 using p62Dok(phospho-Tyr398) Antibody.)

Compare (5 max)

p62Dok, Polyclonal Antibody (Cat# AAA305968)

Full Name

p62Dok (phospho-Tyr398) Antibody

Gene Names

DOK1; P62DOK

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed HeLa cells using NF-kB p65(phospho-Thr435) antibody(, Red).)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NF-kB p65(phospho-Thr435) antibody().)

WB (Western Blot) (Western blot analysis of extracts from Hela cells untreated or treated with TNFa+CA using NFkB-p65(Phospho-Thr435) Antibody.)

Compare (5 max)

NFkappaB-p65, Polyclonal Antibody (Cat# AAA307723)

Full Name

NFkappaB-p65(Phospho-Thr435) Antibody

Gene Names

RELA; p65; NFKB3

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

WB (Western Blot) (Western blot analysis of extracts from NIH-3T3 cells treated with TNF using IKK- alpha/ beta (Phospho-Ser176/177) antibody.The lane on the right is treated with the antigen-specific peptide.)

Compare (5 max)

IKK-alpha/ beta, Polyclonal Antibody (Cat# AAA308010)

Full Name

IKK-alpha/ beta (Phospho-Ser176/177) Antibody

Gene Names

CHUK; IKK1; IKKA; IKBKA; TCF16; NFKBIKA; IKK-alpha

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

< >

<< Previous Next >>

ICC (Immunocytochemistry) (ICC staining Phospho-Smad3 (S423/S425) in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-Phospho-Smad3 (S423/S425) antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Phospho-Smad3 (S423/S425) on different lysates using anti-Phospho-Smad3 (S423/S425) antibody at 1/1, 000 dilution. Positive control: Lane 1: A431 cell lysates-treated with TGF beta Lane 2: A431 cell lysates-untreated)

Compare (5 max)

SMAD3, Monoclonal Antibody (Cat# AAA311006)

Full Name

SMAD3 (Phospho-S423/S425) Antibody

Gene Names

SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436

Reactivity

Human, Mouse

Applications

Flow Cytometry, Immunoprecipitation, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Western Blot

Purity

ProA affinity purified

Pricing

$365/0.05 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $1,990/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Phospho-FOXO3a (S253) antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Phospho-FOXO3a (S253) antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-FOXO3a (S253) antibody. Counter stained with hematoxylin.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Phospho-FOXO3a (S253) antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Phospho-FOXO3a (S253) on different lysates using anti-Phospho-FOXO3a (S253) antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: PC12)

Compare (5 max)

FOXO3a, Monoclonal Antibody (Cat# AAA311007)

Full Name

FOXO3a (Phospho-S253) Antibody

Gene Names

FOXO3; FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Flow Cytometry, Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Western Blot

Purity

ProA affinity purified

Pricing

$365/0.05 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $1,990/5x0.1 mL

< >

<< Previous Next >>

ICC (Immunocytochemistry) (ICC staining Phospho-IKB alpha (S32) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Phospho-IKB alpha (S32) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

WB (Western Blot) (Western blot analysis of Phospho-IKB alpha (S32) on Ags cell lysates using anti-Phospho-IKB alpha (S32) antibody at 1/1, 000 dilution.)

Compare (5 max)

IKB alpha, Monoclonal Antibody (Cat# AAA311009)

Full Name

IKB alpha (Phospho-S32) Antibody

Gene Names

NFKBIA; IKBA; MAD-3; NFKBI

Reactivity

Human, Mouse

Applications

Immunofluorescence, Immunoprecipitation, Immunocytochemistry, Western Blot

Purity

ProA affinity purified

Pricing

$365/0.05 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $1,990/5x0.1 mL

< >

<< Previous Next >>

ICC (Immunocytochemistry) (ICC staining Phospho-EGFR (pY1173) in untreated B-6F1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Phospho-EGFR (pY1173) in untreated A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining (pY1173) in untreated A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti- Phospho-EGFR (pY1173) antibody. Counter stained with hematoxylin.)

Compare (5 max)

EGFR, Monoclonal Antibody (Cat# AAA311014)

Full Name

EGFR (Phospho-pY1173) Antibody

Gene Names

EGFR; ERBB; HER1; mENA; ERBB1; PIG61

Reactivity

Human, Rat

Applications

Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Western Blot

Purity

ProA affinity purified

Pricing

$365/0.05 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $1,990/5x0.1 mL

< >

<< Previous Next >>

ICC (Immunocytochemistry) (ICC staining PDGF Receptor beta (phospho Y740) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining PDGF Receptor beta (phospho Y740) in C2C12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Compare (5 max)

PDGF beta, Monoclonal Antibody (Cat# AAA311021)

Full Name

PDGF Receptor beta (Phospho-Y740) Antibody

Gene Names

Pdgfrb; Pdgfr; CD140b; PDGFR-1; AI528809

Reactivity

Mouse, Rat

Applications

Flow Cytometry, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Western Blot

Purity

ProA affinity purified

Pricing

$365/0.05 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $1,990/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Phospho-PKC zeta (T560) antibody. Counter stained with hematoxylin.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-PKC zeta (T560) antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Phospho-PKC zeta (T560) on mouse eyeball lysates using anti-Phospho-PKC zeta (T560) antibody at 1/1, 000 dilution.)

Compare (5 max)

PKC zeta, Monoclonal Antibody (Cat# AAA311028)

Full Name

PKC zeta (Phospho-T560) Antibody

Gene Names

PRKCZ; PKC2; PKC-ZETA

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunoprecipitation, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Western Blot

Purity

ProA affinity purified

Pricing

$365/0.05 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $1,990/5x0.1 mL

< >

<< Previous Next >>

ICC (Immunocytochemistry) (ICC staining Phospho-Cyclin E1 (T77) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Phospho-Cyclin E1 (T77) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Phospho-Cyclin E1 (T77) in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Phospho-Cyclin E1 (T77) antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Phospho-Cyclin E1 (T77) antibody. Counter stained with hematoxylin.)

Compare (5 max)

Cyclin E1, Monoclonal Antibody (Cat# AAA311030)

Full Name

Cyclin E1 (Phospho-T77) Antibody

Gene Names

CCNE1; CCNE

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Western Blot

Purity

ProA affinity purified

Pricing

$365/0.05 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $1,990/5x0.1 mL

< >

<< Previous Next >>

ICC (Immunocytochemistry) (ICC staining Phospho-c-Jun (T91) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Phospho-c-Jun (T91) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-c-Jun (T91) antibody. Counter stained with hematoxylin.)

Compare (5 max)

c-Jun, Monoclonal Antibody (Cat# AAA311040)

Full Name

c-Jun (Phospho-T91) Antibody

Gene Names

JUN; AP1; AP-1; c-Jun

Reactivity

Human, Mouse, Rat

Applications

Immunoprecipitation, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Western Blot

Purity

ProA affinity purified

Pricing

$365/0.05 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $1,990/5x0.1 mL

< >

<< Previous Next >>

ICC (Immunocytochemistry) (ICC staining Phospho-AKT1 (S124) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Phospho-AKT1 (S124) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Phospho-AKT1 (S124) in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-AKT1 (S124) antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-Phospho-AKT1 (S124) antibody. Counter stained with hematoxylin.)

Compare (5 max)

AKT1, Monoclonal Antibody (Cat# AAA311041)

Full Name

AKT1 (Phospho-S124) Antibody

Gene Names

AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA

Reactivity

Human, Mouse, Rat

Applications

Immunoprecipitation, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Western Blot

Purity

ProA affinity purified

Pricing

$365/0.05 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $1,990/5x0.1 mL

< >

<< Previous Next >>

ICC (Immunocytochemistry) (ICC staining Phospho-SHIP (Y1020) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Phospho-SHIP (Y1020) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Phospho-SHIP (Y1020) in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Compare (5 max)

SHIP, Monoclonal Antibody (Cat# AAA311045)

Full Name

SHIP (Phospho-Y1020) Antibody

Gene Names

INPP5D; SHIP; SHIP1; SHIP-1; hp51CN; SIP-145

Reactivity

Human, Mouse, Rat

Applications

Immunoprecipitation, Immunocytochemistry, Western Blot

Purity

ProA affinity purified

Pricing

$365/0.05 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $1,990/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Confocal immunofluorescent analysis of Phospho-Rb-S811 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)

IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

DB (Dot Blot) (Dot blot analysis of anti-hRb-S811 Phospho-specific Pab on nitrocellulose membrane. 50ng of nonphospho-peptide or phospho-peptide were adsorbed on their respective dots. Antibody working concentration was 0.5ug per ml.)

Compare (5 max)

Phospho-Rb (S811), Polyclonal Antibody (Cat# AAA285351)

Full Name

Phospho-Rb (S811) Antibody

Gene Names

RB1; RB; pRb; OSRC; pp110; p105-Rb; PPP1R130

Reactivity

Human (Predicted Reactivity: Mouse, Rat)

Applications

Immunofluorescence, Immunohistochemistry, Dot Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Cyclin D3 (Phospho-Thr283) Antibody (#A0418).)

WB (Western Blot) (Western blot analysis of extracts from K562 cells treated with UV (5mins), using Cyclin D3 (Phospho-Thr283) Antibody (#A0418).)

Compare (5 max)

Cyclin D3 (Phospho-Thr283), Polyclonal Antibody (Cat# AAA285355)

Full Name

Cyclin D3 (Phospho-Thr283) Antibody

Reactivity

Human, mouse, rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot) (The anti-Phospho-Rad9-S387 Pab is used in Western blot to detect Phospho-Rad9-S387 in Y79 (left) and Hela (right) tissue lysate)

Compare (5 max)

Phospho-RAD9 (S387), Polyclonal Antibody (Cat# AAA285295)

Full Name

Phospho-RAD9 (S387) Antibody

Gene Names

RAD9A; RAD9

Reactivity

Human (Predicted Reactivity: Monkey)

Applications

Immunohistochemistry, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of extracts from T47D cells,untreated or treated with NRG using Phospho-ErBB3(Tyr1289)(left) or ErBB3 antibody(right).)

DB (Dot Blot) (Dot blot analysis of anti-Phospho-ERBB3 (Tyr1289) Antibody Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

Compare (5 max)

Phospho-ERBB3 (Y1289), Polyclonal Antibody (Cat# AAA289231)

Full Name

Phospho-ERBB3 (Y1289) Antibody

Gene Names

ERBB3; HER3; LCCS2; ErbB-3; c-erbB3; erbB3-S; MDA-BF-1; c-erbB-3; p180-ErbB3; p45-sErbB3; p85-sErbB3

Reactivity

Human

Applications

Dot Blot, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)

WB (Western Blot) (Western blot analysis of lysate from Jurkat cells)

Compare (5 max)

ZFP598, Polyclonal Antibody (Cat# AAA312931)

Full Name

ZFP598 (Phospho Tyr306) Polyclonal Antibody

Reactivity

Human, Mouse

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistochemisry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot) (The anti-Phospho-MYT1-T495 Antibody is used in Western blot to detect Phospho-MYT1-T495 in HL60 (left) and SK-BR-3 (right) tissue lysates.)

DB (Dot Blot) (Dot blot analysis of anti-Phospho-MYT1-T495 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

Compare (5 max)

Phospho-MYT1 (T495), Polyclonal Antibody (Cat# AAA285563)

Full Name

Phospho-MYT1 (T495) Antibody

Gene Names

PKMYT1; MYT1; PPP1R126

Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Dot Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

What Are Phospho Antibodies?

Protein phosphorylation is a process where a phosphate group is added to certain amino acid residues of a protein – usually serine (S), threonine (T), or tyrosine (Y) - by enzymes called kinases. This process is integral in controlling cellular signaling, cellular growth, and other biological functions.

Our catalog includes a wide range of phospho-specific antibodies that can accurately detect this important marker. They perform strongly in widely-used laboratory applications such as Western blot, flow cytometry, immunohistochemistry, and immunofluorescence microscopy. We value your trust in us and are committed to providing top-quality products and services. All of our antibodies are guaranteed to work for the applications and species indicated on our website & associated product pages.

What Are The Key Applications of Phospho Antibodies?

1. Western Blotting

One of the first steps a researcher can take in utilizing these phospho-specific antibodies, is to check if the antibody works using a technique referred to as “Western blot”. For those unfamiliar, Western Blot aids in showing whether the protein that the antibody recognizes is appearing at the correct/expected size. These phospho-specific antibodies should also be able to detect changes in the target protein’s phosphorylation (on/off state) when cells are stimulated in certain ways.

2. Staining of Fixed Cells (Immunocytochemistry)

Another routine use of these phospho-specific antibodies, is to test if the antibody is able to demonstrate similar performance when used on fixed cells (intact cells that have been preserved) as it did in the Western blot tests. It is an important aspect in many cases to confirm that the antibody works in actual intact cell samples. Ideally, the method used for cellular fixation should be the same as what is used in pathology labs (like using 10% formalin). To check if the antibody works well in tissue sections (FFPE), researchers will often test it on fixed cells that are processed similar to tissue samples.

3. Specificity Tests Using Peptides

In order to make sure that the antibody is only binding to the right target:

• Laboratory technicians will mix the antibody with phospho-peptides (short segments of the protein containing the phosphate group modification).


• If the antibody signal disappears, it is confirmation that it is binding to the correct phosphorylated location.


• A more robust test is to use both the phosphorylated and non-phosphorylated (dephosphorylated) versions of the protein. The antibody should react only with the phosphorylated one.


• Another method sometimes utilized is to treat the sample with an enzyme, such as alkaline phosphatase, that specifically removes phosphate groups. If the antibody signal disappears after this, it also confirms specificity.

4. Genetic Confirmation

As a final step, scientists can genetically manipulate the nucleotide sequence and alter the target protein by removing the exact site where phosphorylation happens. If the antibody no longer appears to detect the modified protein, it is strong evidence supporting the antibody being specific for that phosphorylated site.

Why Buy Phospho Antibodies Through Us?

• The production laboratory adheres to strict and consistent protocols prior to releasing any of these phospho-specific antibodies:


• Standard methods and proper controls in all tests to ensure high quality.


• These antibodies are tested and validated in different cell types and species.


• High quality control criterion to ensure each batch is consistent, so you will obtain reliable results every time.

FAQ

1. What Are Phospho-Specific Antibodies?

Phospho-specific antibodies are made to detect proteins only when they have a phosphate group linked to a specific amino acid residue. This empowers scientists understand if a protein is "turned on" or active, based on its phosphorylation state.

2. How to Detect Phosphorylated Proteins in a Western Blot?

To find out if a protein is phosphorylated using Western blot:

• Use a phospho-specific antibody that binds only to the phosphorylated form of the protein.


• You can also use a “regular” antibody for the same amino acid sequence of the protein that the phospho-specific antibody is binding to (but in this case, this antibody will not bind if there is a phosphate group present) in order to compare how much of it is phosphorylated versus how much is non-phosphorylated (or “total” protein, if the “normal” antibody’s epitopes are non-phospho-site-specific).

3. How to Choose the Best Antibody?

Here are some simple tips to help you pick the right antibody:

• Know your target


• Match your sample characteristics


• Confirm the intended use is appropriate


• Check “host” and “type”


• Check the “quality” of the presented data/images


• Appraise whether the available validation meets your needs