DB (Dot Blot)
(Dot blot analysis of Phospho-IKKB-S672 Antibody Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)
Rabbit Phospho-IKKB (S672) Polyclonal Antibody | anti-IKBKB antibody
Phospho-IKKB (S672) Antibody
Gene Names
IKBKB; IKK2; IKKB; IMD15; NFKBIKB; IKK-beta
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rat)
Applications
ELISA, Dot Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Phospho-IKKB (S672), Antibody; Phospho-IKKB (S672) Antibody; Inhibitor of nuclear factor kappa-B kinase subunit beta; I-kappa-B-kinase beta; IKK-B; IKK-beta; IkBKB; I-kappa-B kinase 2; IKK2; Nuclear factor NF-kappa-B inhibitor kinase beta; NFKBIKB; IKBKB; IKKB; anti-IKBKB antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This IKKB Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S672 of human IKKB.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
697
Applicable Applications for anti-IKBKB antibody
ELISA, DB (Dot Blot)
Function
Serine kinase that plays an essential role in the NF- kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. In addition to the NF-kappa-B inhibitors, phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE. IKK-related kinase phosphorylations may prevent the overproduction of inflammatory mediators since they exert a negative regulation on canonical IKKs. Phosphorylates FOXO3, mediating the TNF- dependent inactivation of this pro-apoptotic transcription factor. Also phosphorylates other substrates including NCOA3, BCL10 and IRS1. Within the nucleus, acts as an adapter protein for NFKBIA degradation in UV-induced NF-kappa-B activation.
Cellular Location
Cytoplasm. Nucleus. Membrane raft. Note=Colocalized with DPP4 in membrane rafts.
Tissue Location
Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
DB (Dot Blot)
(Dot blot analysis of Phospho-IKKB-S672 Antibody Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)
DB (Dot Blot)
(Dot blot analysis of Phospho-IKKB-S672 Antibody Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)
Related Product Information for anti-IKBKB antibody
NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL
(MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the
NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins
(NFKBIA, MIM 164008, or NFKBIB, MIM 604495), which inactivate
NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of
serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM
600664, or IKBKB) marks them for destruction via the ubiquitination
pathway, thereby allowing activation of the NF-kappa-B complex.
Activated NFKB complex translocates into the nucleus and binds DNA
at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or
5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G
purine; and Y is a C or T pyrimidine).
(MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the
NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins
(NFKBIA, MIM 164008, or NFKBIB, MIM 604495), which inactivate
NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of
serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM
600664, or IKBKB) marks them for destruction via the ubiquitination
pathway, thereby allowing activation of the NF-kappa-B complex.
Activated NFKB complex translocates into the nucleus and binds DNA
at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or
5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G
purine; and Y is a C or T pyrimidine).
Product Categories/Family for anti-IKBKB antibody
References
Yatherajam, G., et al. J. Immunol. 185(5):2665-2669(2010)
Kenneth, N.S., et al. EMBO J. 29(17):2966-2978(2010)
Zhao, M., et al. J. Biol. Chem. 285(32):24372-24380(2010)
Niida, M., et al. Mol. Immunol. 47(14):2378-2387(2010)
Schuurhof, A., et al. Pediatr. Pulmonol. 45(6):608-613(2010)
Kenneth, N.S., et al. EMBO J. 29(17):2966-2978(2010)
Zhao, M., et al. J. Biol. Chem. 285(32):24372-24380(2010)
Niida, M., et al. Mol. Immunol. 47(14):2378-2387(2010)
Schuurhof, A., et al. Pediatr. Pulmonol. 45(6):608-613(2010)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
86564
NCBI Official Full Name
inhibitor of nuclear factor kappa-B kinase subunit beta isoform 2
NCBI Official Synonym Full Names
inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
NCBI Official Symbol
IKBKB
NCBI Official Synonym Symbols
IKK2; IKKB; IMD15; NFKBIKB; IKK-beta
NCBI Protein Information
inhibitor of nuclear factor kappa-B kinase subunit beta
UniProt Protein Name
Inhibitor of nuclear factor kappa-B kinase subunit beta
UniProt Gene Name
IKBKB
UniProt Synonym Gene Names
IKKB; I-kappa-B-kinase beta; IKK-B; IKK-beta; IkBKB; IKK2; NFKBIKB
UniProt Entry Name
IKKB_HUMAN
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Product Notes
The IKBKB ikbkb (Catalog #AAA283856) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-IKKB (S672) Antibody reacts with Human (Predicted Reactivity: Bovine, Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's Phospho-IKKB (S672) can be used in a range of immunoassay formats including, but not limited to, ELISA, DB (Dot Blot). Researchers should empirically determine the suitability of the IKBKB ikbkb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospho-IKKB (S672), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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