Phospho Antibodies

Phospho-specific antibodies’ typical purpose is to enable researchers to detect changes in proteins. They will exclusively bind to the amino acid sequence on a protein that has been phosphorylated (which is both a physical & chemical change) and do not bind to the same amino acid sequence on said protein if it lacks said phosphorylation. This aids in being able to clearly see and understand the data produced from this particular protein modification.

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WB (Western Blot) (Western blot analysis of lysates from A549 cells treated with PMA 125ng/ML 30', using p70 S6 Kinase (Phospho-Ser424) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using p70 S6 Kinase (Phospho-Ser424) Antibody. The picture on the right is blocked with the phospho peptide.)

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p70 S6 Kinase, Polyclonal Antibody (Cat# AAA316559)

Full Name

p70 S6 Kinase (Phospho-Ser424) Antibody

Gene Names

RPS6KB1; S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from K562 cells treated with serum 10%, using Retinoblastoma (Phospho-Ser807) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Retinoblastoma (Phospho-Ser807) Antibody. The picture on the right is blocked with the phospho peptide.)

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Retinoblastoma, Polyclonal Antibody (Cat# AAA316569)

Full Name

Retinoblastoma (Phospho-Ser807) Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from COLO205 cells treated with serum 15% 10', using Src (Phospho-Tyr418) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using Src (Phospho-Tyr418) Antibody. The picture on the right is blocked with the phospho peptide.)

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Src, Polyclonal Antibody (Cat# AAA316575)

Full Name

Src (Phospho-Tyr418) Antibody

Gene Names

SRC; ASV; SRC1; THC6; c-SRC; p60-Src

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from COS7 cells treated with PMA 125ng/ml 30', using Src (Phospho-Tyr529) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Src (Phospho-Tyr529) Antibody. The picture on the right is blocked with the phospho peptide.)

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Src, Polyclonal Antibody (Cat# AAA316576)

Full Name

Src (Phospho-Tyr529) Antibody

Gene Names

SRC; ASV; SRC1; THC6; c-SRC; p60-Src

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from 293 cells, 3T3 cells treated with UV (15mins) and Jurkat cells treated with eto (25uM, 24hours), using STAT1 (Phospho-Ser727) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using STAT1 (Phospho-Ser727) Antibody. The picture on the right is blocked with the phospho peptide.)

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STAT1, Polyclonal Antibody (Cat# AAA316577)

Full Name

STAT1 (Phospho-Ser727) Antibody

Gene Names

STAT1; CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT91

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from 293 cells, using Stathmin 1 (Phospho-Ser15) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Stathmin 1 (Phospho-Ser15) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of COS7 cells, using Stathmin 1 (Phospho-Ser15) Antibody. The picture on the right is blocked with the phospho peptide.)

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Stathmin 1, Polyclonal Antibody (Cat# AAA316585)

Full Name

Stathmin 1 (Phospho-Ser15) Antibody

Gene Names

STMN1; Lag; SMN; OP18; PP17; PP19; PR22; LAP18; C1orf215

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HeLa cells treated with nocodazole, using Stathmin 1 (Phospho-Ser37) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Stathmin 1 (Phospho-Ser37) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

Stathmin 1, Polyclonal Antibody (Cat# AAA316587)

Full Name

Stathmin 1 (Phospho-Ser37) Antibody

Gene Names

STMN1; Lag; SMN; OP18; PP17; PP19; PR22; LAP18; C1orf215

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from 293 cells treated with serum 10% 15', using Tau (Phospho-Ser356) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using Tau (Phospho-Ser356) Antibody. The picture on the right is blocked with the phospho peptide.)

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Tau, Polyclonal Antibody (Cat# AAA316588)

Full Name

Tau (Phospho-Ser356) Antibody

Gene Names

MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from mouse brain, using Tau (Phospho-Ser422) Antibody. The lane on the left is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using Tau (Phospho-Ser422) Antibody. The picture on the right is blocked with the phospho peptide.)

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Tau, Polyclonal Antibody (Cat# AAA316589)

Full Name

Tau (Phospho-Ser422) Antibody

Gene Names

MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from NIH/3T3 cells treated with forskolin 40 muM 30', using VASP (Phospho-Ser157) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human tonsil, using VASP (Phospho-Ser157) Antibody. The picture on the right is blocked with the phospho peptide.)

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VASP, Polyclonal Antibody (Cat# AAA316590)

Full Name

VASP (Phospho-Ser157) Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from RAW264.7 cells, using VASP (Phospho-Ser238) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human tonsil, using VASP (Phospho-Ser238) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

VASP, Polyclonal Antibody (Cat# AAA316591)

Full Name

VASP (Phospho-Ser238) Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HepG2 cells treated with Na3VO4 0.3nM 40', using VEGFR2 (Phospho-Tyr1214) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using VEGFR2 (Phospho-Tyr1214) Antibody. The picture on the right is blocked with the phospho peptide.)

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VEGFR2, Polyclonal Antibody (Cat# AAA316593)

Full Name

VEGFR2 (Phospho-Tyr1214) Antibody

Gene Names

KDR; FLK1; CD309; VEGFR; VEGFR2

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from SK-OV3 cells, using VEGFR2 (Phospho-Tyr951) Antibody. The lane on the left is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using VEGFR2 (Phospho-Tyr951) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

VEGFR2, Polyclonal Antibody (Cat# AAA316594)

Full Name

VEGFR2 (Phospho-Tyr951) Antibody

Gene Names

KDR; FLK1; CD309; VEGFR; VEGFR2

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

Application Data (The TFact p53 (Phospho-Ser315) DNA-Binding ELISA detects active p53 (Phospho-Ser315) in Hela, MCF7 and HepG2 Nuclear Extract. The Hela, MCF7 and HepG2 cells were grown 3 days in DMEM with 10% FBS and harvested for nuclear extract.)

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p53, ELISA Kit (Cat# AAA315961)

Full Name

p53 (Phospho-Ser315) TFact DNA-Binding ELISA Kit

Gene Names

TP53; P53; BCC7; LFS1; TRP53

Reactivity

Human, Mouse

Pricing

$635/1 Kit $1,230/2x1 Kit $1,800/3x1 Kit $2,335/4x1 Kit $2,905/5x1 Kit

Application Data (The TFact MEF2A (Phospho-Ser408) DNA-Binding ELISA detects active MEF2A (Phospho-Ser408) in Hela Nuclear Extract. The Hela cells were grown 3 days in DMEM with 10% FBS and harvested for nuclear extract. The Hela cells were stimulated by PMA (200nM) before harvest.)

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MEF2A, ELISA Kit (Cat# AAA315983)

Full Name

MEF2A (Phospho-Ser408) TFact DNA-Binding ELISA Kit

Gene Names

MEF2A; mef2; ADCAD1; RSRFC4; RSRFC9

Reactivity

Human, Mouse, Rat

Pricing

$635/1 Kit $1,230/2x1 Kit $1,800/3x1 Kit $2,335/4x1 Kit $2,905/5x1 Kit

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WB (Western Blot) (Western blot analysis of lysates from NIH/3T3 cells, using CaMK2 alpha/beta/delta (Phospho-Thr305) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using CaMK2 alpha/beta/delta (Phospho-Thr305) Antibody. The picture on the right is blocked with the phospho peptide.)

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CaMK2 alpha/beta/delta, Polyclonal Antibody (Cat# AAA316056)

Full Name

CaMK2 alpha/beta/delta (Phospho-Thr305) Antibody

Gene Names

CAMK2A; CAMKA; MRD53

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from COS7 cells, using GABA-RB (Phospho-Ser434) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using GABA-RB (Phospho-Ser434) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

GABA-RB, Polyclonal Antibody (Cat# AAA316061)

Full Name

GABA-RB (Phospho-Ser434) Antibody

Gene Names

GABRB1; EIEE45

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HeLa cells, using GSK3 alpha/beta (Phospho-Tyr279/216) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using GSK3 alpha/beta (Phospho-Tyr279/216) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

GSK3 alpha/beta, Polyclonal Antibody (Cat# AAA316062)

Full Name

GSK3 alpha/beta (Phospho-Tyr279/216) Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HeLa cells treated with TNF-a 20ng/ml 5', using IkappaB-beta (Phospho-Ser23) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using IkappaB-beta (Phospho-Ser23) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells treated with TNF-a 20nM 15', using IkappaB-beta (Phospho-Ser23) Antibody. The picture on the right is blocked with the phospho peptide.)

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IkappaB-beta, Polyclonal Antibody (Cat# AAA316064)

Full Name

IkappaB-beta (Phospho-Ser23) Antibody

Gene Names

NFKBIB; IKBB; TRIP9

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from Jurkat cells treated with TNF-a 20ng/ml 30', using IkappaB-epsilon (Phospho-Ser22) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using IkappaB-epsilon (Phospho-Ser22) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells, using IkappaB-epsilon (Phospho-Ser22) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

IkappaB-epsilon, Polyclonal Antibody (Cat# AAA316065)

Full Name

IkappaB-epsilon (Phospho-Ser22) Antibody

Gene Names

NFKBIE; IKBE

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from 293 cells treated with Forskolin 40nM 30', using Myc (Phospho-Ser62) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Myc (Phospho-Ser62) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells treated with Forskolin 40nM 30', using Myc (Phospho-Ser62) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

Myc, Polyclonal Antibody (Cat# AAA316070)

Full Name

Myc (Phospho-Ser62) Antibody

Gene Names

MYC; MRTL; MYCC; c-Myc; bHLHe39

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from NIH/3T3 cells, using Trk B (Phospho-Tyr515) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using Trk B (Phospho-Tyr515) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

Trk B, Polyclonal Antibody (Cat# AAA316083)

Full Name

Trk B (Phospho-Tyr515) Antibody

Gene Names

NTRK2; OBHD; TRKB; trk-B; EIEE58; GP145-TrkB

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from 293 cells treated with EGF 200ng/ml 5', using Acetyl-CoA Carboxylase (Phospho-Ser80) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Acetyl-CoA Carboxylase (Phospho-Ser80) Antibody. The picture on the right is blocked with the phospho peptide.)

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Acetyl-CoA Carboxylase, Polyclonal Antibody (Cat# AAA316088)

Full Name

Acetyl-CoA Carboxylase (Phospho-Ser80) Antibody

Gene Names

ACACA; ACC; ACAC; ACC1; ACCA; ACACAD

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from Jurkat cells, using Caspase 8 (Phospho-Ser347) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using Caspase 8 (Phospho-Ser347) Antibody. The picture on the right is blocked with the phospho peptide.)

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Caspase 8, Polyclonal Antibody (Cat# AAA316093)

Full Name

Caspase 8 (Phospho-Ser347) Antibody

Gene Names

CASP8; CAP4; MACH; MCH5; FLICE; ALPS2B; Casp-8

Reactivity

Human, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from 293 cells treated with UV 15', using COT (Phospho-Thr290) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using COT (Phospho-Thr290) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HUVEC cells, using COT (Phospho-Thr290) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

COT, Polyclonal Antibody (Cat# AAA316095)

Full Name

COT (Phospho-Thr290) Antibody

Gene Names

MAP3K8; COT; EST; ESTF; TPL2; AURA2; MEKK8; Tpl-2; c-COT

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from COS7 cells, using CrkL (Phospho-Tyr207) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using CrkL (Phospho-Tyr207) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HUVEC cells, using CrkL (Phospho-Tyr207) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

CrkL, Polyclonal Antibody (Cat# AAA316098)

Full Name

CrkL (Phospho-Tyr207) Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HeLa cells treated with Adriamycin 0.5ng/ml 24h, using HSL (Phospho-Ser855/554) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using HSL (Phospho-Ser855/554) Antibody. The picture on the right is blocked with the phospho peptide.)

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HSL, Polyclonal Antibody (Cat# AAA316105)

Full Name

HSL (Phospho-Ser855/554) Antibody

Gene Names

LIPE; HSL; LHS; AOMS4; FPLD6

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from NIH/3T3 cells, using p130 Cas (Phospho-Tyr410) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using p130 Cas (Phospho-Tyr410) Antibody. The picture on the right is blocked with the phospho peptide.)

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p130 Cas, Polyclonal Antibody (Cat# AAA316108)

Full Name

p130 Cas (Phospho-Tyr410) Antibody

Gene Names

BCAR1; CAS; CAS1; CASS1; CRKAS; P130Cas

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from A431 cells, using PLCB3 (Phospho-Ser1105) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using PLCB3 (Phospho-Ser1105) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

PLCB3, Polyclonal Antibody (Cat# AAA316109)

Full Name

PLCB3 (Phospho-Ser1105) Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from COS7 cells treated with EGF 200ng/ml 30', using PLCG1 (Phospho-Tyr771) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PLCG1 (Phospho-Tyr771) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

PLCG1, Polyclonal Antibody (Cat# AAA316110)

Full Name

PLCG1 (Phospho-Tyr771) Antibody

Gene Names

PLCG1; PLC1; NCKAP3; PLC-II; PLC148; PLCgamma1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from COS7 cells treated with EGF 200ng/ml 30', using POLR2A (Phospho-Ser1619) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using POLR2A (Phospho-Ser1619) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells treated with PMA 125ng/ml 30', using POLR2A (Phospho-Ser1619) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

POLR2A, Polyclonal Antibody (Cat# AAA316113)

Full Name

POLR2A (Phospho-Ser1619) Antibody

Gene Names

POLR2A; RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from 293 cells treated with EGF 200ng/ml 30', using SEPARASE (Phospho-Ser801) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using SEPARASE (Phospho-Ser801) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HUVEC cells, using SEPARASE (Phospho-Ser801) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

SEPARASE, Polyclonal Antibody (Cat# AAA316116)

Full Name

SEPARASE (Phospho-Ser801) Antibody

Gene Names

ESPL1; ESP1; SEPA

Reactivity

Human, Mouse

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from Jurkat cells treated with Paclitaxel 1uM 24h, using C-RAF (Phospho-Tyr341) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human pancreas, using C-RAF (Phospho-Tyr341) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

C-RAF, Polyclonal Antibody (Cat# AAA316219)

Full Name

C-RAF (Phospho-Tyr341) Antibody

Gene Names

RAF1; NS5; CRAF; Raf-1; c-Raf; CMD1NN

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from HUVEC cells treated with anisomycin 25ug/ml 30', using SF1 (Phospho-Ser82) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using SF1 (Phospho-Ser82) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells, using SF1 (Phospho-Ser82) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

SF1, Polyclonal Antibody (Cat# AAA316222)

Full Name

SF1 (Phospho-Ser82) Antibody

Gene Names

SF1; BBP; MBBP; ZFM1; ZNF162; D11S636; ZCCHC25

Reactivity

Human, Mouse

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from NIH/3T3 cells treated with EGF 200ng/ml 30', using TEBP (Phospho-Ser113) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using TEBP (Phospho-Ser113) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

TEBP, Polyclonal Antibody (Cat# AAA316230)

Full Name

TEBP (Phospho-Ser113) Antibody

Gene Names

PTGES3; P23; TEBP; cPGES

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HepG2 cells treated with Anisomycin 25ug/ml 30', using Tyrosine Hydroxylase (Phospho-Ser8) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using Tyrosine Hydroxylase (Phospho-Ser8) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

Tyrosine Hydroxylase, Polyclonal Antibody (Cat# AAA316235)

Full Name

Tyrosine Hydroxylase (Phospho-Ser8) Antibody

Gene Names

Th; The

Reactivity

Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from NIH/3T3 cells treated with Insulin 0.01U/ml 15', using WAVE1 (Phospho-Tyr125) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using WAVE1 (Phospho-Tyr125) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

WAVE1, Polyclonal Antibody (Cat# AAA316238)

Full Name

WAVE1 (Phospho-Tyr125) Antibody

Gene Names

WASF1; WAVE; SCAR1; WAVE1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from Jurkat cells treated with Ca+ 40nM 30', using ZAP-70 (Phospho-Tyr315) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human lymph node, using ZAP-70 (Phospho-Tyr315) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

ZAP-70, Polyclonal Antibody (Cat# AAA316240)

Full Name

ZAP-70 (Phospho-Tyr315) Antibody

Gene Names

ZAP70; SRK; STD; TZK; STCD; IMD48; ADMIO2; ZAP-70

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from NIH/3T3 cells treated with TNF-a 20ng/ml 30', using Akt (Phospho-Thr72) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human ovary, using Akt (Phospho-Thr72) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

Akt, Polyclonal Antibody (Cat# AAA316244)

Full Name

Akt (Phospho-Thr72) Antibody

Gene Names

AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from COS7 cells treated with Calyculin 50nM 30', using ATP-Citrate Lyase (Phospho-Ser454) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using ATP-Citrate Lyase (Phospho-Ser454) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

ATP-Citrate Lyase, Polyclonal Antibody (Cat# AAA316247)

Full Name

ATP-Citrate Lyase (Phospho-Ser454) Antibody

Gene Names

ACLY; ACL; ATPCL; CLATP

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from HUVEC cells treated with UV 15', using Chk1 (Phospho-Ser296) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using Chk1 (Phospho-Ser296) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

Chk1, Polyclonal Antibody (Cat# AAA316251)

Full Name

Chk1 (Phospho-Ser296) Antibody

Gene Names

CHEK1; CHK1

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from Jurkat cells treated with UV 15', using Cyclin B1 (Phospho-Ser147) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human placenta, using Cyclin B1 (Phospho-Ser147) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

Cyclin B1, Polyclonal Antibody (Cat# AAA316253)

Full Name

Cyclin B1 (Phospho-Ser147) Antibody

Gene Names

CCNB1; CCNB

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from HeLa cells treated with PMA 125ng/ml 30', using LKB1 (Phospho-Ser428) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using LKB1 (Phospho-Ser428) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells, using LKB1 (Phospho-Ser428) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

LKB1, Polyclonal Antibody (Cat# AAA316257)

Full Name

LKB1 (Phospho-Ser428) Antibody

Gene Names

STK11; PJS; LKB1; hLKB1

Reactivity

Human, Mouse

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using mTOR (Phospho-Ser2481) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells, using mTOR (Phospho-Ser2481) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

mTOR, Polyclonal Antibody (Cat# AAA316264)

Full Name

mTOR (Phospho-Ser2481) Antibody

Gene Names

MTOR; SKS; FRAP; FRAP1; FRAP2; RAFT1; RAPT1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from HepG2 cells, using NPM (Phospho-Thr199) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using NPM (Phospho-Thr199) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells treated with EGF 200nM 5', using NPM (Phospho-Thr199) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

NPM, Polyclonal Antibody (Cat# AAA316268)

Full Name

NPM (Phospho-Thr199) Antibody

Gene Names

NPM1; B23; NPM

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from A549 cells treated with PMA 125ng/ml 30' and HT29 cells treated with serum 20% 15', using PKD1/2/3/PKC mu (Phospho-Ser738+Ser742) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human heart, using PKD1/2/3/PKC mu (Phospho-Ser738+Ser742) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

PKD1/2/3/PKC mu, Polyclonal Antibody (Cat# AAA316279)

Full Name

PKD1/2/3/PKC mu (Phospho-Ser738+Ser742) Antibody

Gene Names

PRKD1; PKD; PKCM; CHDED; PRKCM; PKC-MU

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using PLD1 (Phospho-Ser561) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HepG2 cells, using PLD1 (Phospho-Ser561) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

PLD1, Polyclonal Antibody (Cat# AAA316281)

Full Name

PLD1 (Phospho-Ser561) Antibody

Gene Names

PLD1; CVDD

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from Jurkat cells treated with UV 15', using 14-3-3 zeta/delta (Phospho-Thr232) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using 14-3-3 zeta/delta (Phospho-Thr232) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

14-3-3 zeta/delta, Polyclonal Antibody (Cat# AAA316285)

Full Name

14-3-3 zeta/delta (Phospho-Thr232) Antibody

Gene Names

YWHAZ; HEL4; YWHAD; KCIP-1; HEL-S-3; HEL-S-93; 14-3-3-zeta

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from HepG2 cells treated with Adriamycin 0.5uM 5h, using Ataxin 1 (Phospho-Ser776) Antibody. The lane on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells, using Ataxin 1 (Phospho-Ser776) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

Ataxin 1, Polyclonal Antibody (Cat# AAA316292)

Full Name

Ataxin 1 (Phospho-Ser776) Antibody

Gene Names

ATXN1; ATX1; SCA1; D6S504E

Reactivity

Human, Mouse

Applications

Immunofluorescence, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from K562 cells treated with insulin 0.01U/ml 15', using p57 Kip2 (Phospho-Thr310) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human placenta, using p57 Kip2 (Phospho-Thr310) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HUVEC cells treated with serum 20% 30', using p57 Kip2 (Phospho-Thr310) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

p57 Kip2, Polyclonal Antibody (Cat# AAA316375)

Full Name

p57 Kip2 (Phospho-Thr310) Antibody

Gene Names

CDKN1C; BWS; WBS; p57; BWCR; KIP2; p57Kip2

Reactivity

Human, Mouse

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

What Are Phospho Antibodies?

Protein phosphorylation is a process where a phosphate group is added to certain amino acid residues of a protein – usually serine (S), threonine (T), or tyrosine (Y) - by enzymes called kinases. This process is integral in controlling cellular signaling, cellular growth, and other biological functions.

Our catalog includes a wide range of phospho-specific antibodies that can accurately detect this important marker. They perform strongly in widely-used laboratory applications such as Western blot, flow cytometry, immunohistochemistry, and immunofluorescence microscopy. We value your trust in us and are committed to providing top-quality products and services. All of our antibodies are guaranteed to work for the applications and species indicated on our website & associated product pages.

What Are The Key Applications of Phospho Antibodies?

1. Western Blotting

One of the first steps a researcher can take in utilizing these phospho-specific antibodies, is to check if the antibody works using a technique referred to as “Western blot”. For those unfamiliar, Western Blot aids in showing whether the protein that the antibody recognizes is appearing at the correct/expected size. These phospho-specific antibodies should also be able to detect changes in the target protein’s phosphorylation (on/off state) when cells are stimulated in certain ways.

2. Staining of Fixed Cells (Immunocytochemistry)

Another routine use of these phospho-specific antibodies, is to test if the antibody is able to demonstrate similar performance when used on fixed cells (intact cells that have been preserved) as it did in the Western blot tests. It is an important aspect in many cases to confirm that the antibody works in actual intact cell samples. Ideally, the method used for cellular fixation should be the same as what is used in pathology labs (like using 10% formalin). To check if the antibody works well in tissue sections (FFPE), researchers will often test it on fixed cells that are processed similar to tissue samples.

3. Specificity Tests Using Peptides

In order to make sure that the antibody is only binding to the right target:

• Laboratory technicians will mix the antibody with phospho-peptides (short segments of the protein containing the phosphate group modification).


• If the antibody signal disappears, it is confirmation that it is binding to the correct phosphorylated location.


• A more robust test is to use both the phosphorylated and non-phosphorylated (dephosphorylated) versions of the protein. The antibody should react only with the phosphorylated one.


• Another method sometimes utilized is to treat the sample with an enzyme, such as alkaline phosphatase, that specifically removes phosphate groups. If the antibody signal disappears after this, it also confirms specificity.

4. Genetic Confirmation

As a final step, scientists can genetically manipulate the nucleotide sequence and alter the target protein by removing the exact site where phosphorylation happens. If the antibody no longer appears to detect the modified protein, it is strong evidence supporting the antibody being specific for that phosphorylated site.

Why Buy Phospho Antibodies Through Us?

• The production laboratory adheres to strict and consistent protocols prior to releasing any of these phospho-specific antibodies:


• Standard methods and proper controls in all tests to ensure high quality.


• These antibodies are tested and validated in different cell types and species.


• High quality control criterion to ensure each batch is consistent, so you will obtain reliable results every time.

FAQ

1. What Are Phospho-Specific Antibodies?

Phospho-specific antibodies are made to detect proteins only when they have a phosphate group linked to a specific amino acid residue. This empowers scientists understand if a protein is "turned on" or active, based on its phosphorylation state.

2. How to Detect Phosphorylated Proteins in a Western Blot?

To find out if a protein is phosphorylated using Western blot:

• Use a phospho-specific antibody that binds only to the phosphorylated form of the protein.


• You can also use a “regular” antibody for the same amino acid sequence of the protein that the phospho-specific antibody is binding to (but in this case, this antibody will not bind if there is a phosphate group present) in order to compare how much of it is phosphorylated versus how much is non-phosphorylated (or “total” protein, if the “normal” antibody’s epitopes are non-phospho-site-specific).

3. How to Choose the Best Antibody?

Here are some simple tips to help you pick the right antibody:

• Know your target


• Match your sample characteristics


• Confirm the intended use is appropriate


• Check “host” and “type”


• Check the “quality” of the presented data/images


• Appraise whether the available validation meets your needs