Phospho Antibodies

Phospho-specific antibodies’ typical purpose is to enable researchers to detect changes in proteins. They will exclusively bind to the amino acid sequence on a protein that has been phosphorylated (which is both a physical & chemical change) and do not bind to the same amino acid sequence on said protein if it lacks said phosphorylation. This aids in being able to clearly see and understand the data produced from this particular protein modification.

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IF (Immunofluorescence) (Confocal immunofluorescent analysis of Phospho-TSC2-pS939 Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)

DB (Dot Blot) (Dot blot analysis of anti-TSC2-pS939 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

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Phospho-TSC2 (S939), Polyclonal Antibody (Cat# AAA288787)

Full Name

Phospho-TSC2 (S939) Antibody

Gene Names

TSC2; LAM; TSC4; PPP1R160

Reactivity

Human (Predicted Reactivity: Mouse)

Applications

Immunofluorescence, Dot Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot) (The anti-Phospho-CDC25A-S293 Pab is used in Western blot to detect Phospho-CDC25A-S293 in cells transfected with wild type or mutant S293A of CDC25A. Data courtesy of Dr. Tiebang Kang of Washington University, St. Louis, MO.)

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Phospho-CDC25A (S293), Polyclonal Antibody (Cat# AAA289059)

Full Name

Phospho-CDC25A (S293) Antibody

Gene Names

CDC25A; CDC25A2

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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DB (Dot Blot) (Dot blot analysis of anti-TSC2-pS664 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

IF (Immunofluorescence) (Confocal immunofluorescent analysis of Phospho-TSC2-pS664 Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).)

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Phospho-TSC2 (S664), Polyclonal Antibody (Cat# AAA288871)

Full Name

Phospho-TSC2 (S664) Antibody

Gene Names

TSC2; LAM; TSC4; PPP1R160

Reactivity

Human

Applications

Dot Blot, Immunofluorescence

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot) (The anti-Phospho-MAPKAPK5-S93 Pab is used in Western blot to detect Phospho-MAPKAPK5-S93 in mouse heart tissue lysate. PAD4(arrow) was detected using the purified Pab.)

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Phospho-MAPKAPK5 (S93), Polyclonal Antibody (Cat# AAA289717)

Full Name

Phospho-MAPKAPK5 (S93) Antibody

Gene Names

MAPKAPK5; MK5; MK-5; PRAK; MAPKAP-K5

Reactivity

Human, mouse

Applications

Immunohistochemistry, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

DB (Dot Blot) (Dot blot analysis of anti-Phospho-TERT-pS1125 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

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Phospho-TERT (S1125), Polyclonal Antibody (Cat# AAA289274)

Full Name

Phospho-TERT (S1125) Antibody

Gene Names

TERT; TP2; TRT; CMM9; EST2; TCS1; hTRT; DKCA2; DKCB4; hEST2; PFBMFT1

Reactivity

Human

Applications

Dot Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot) (Phospho-HER4-Y1188 Antibody was used to detect Phospho-HER4-Y1188 in FG pancreatic carcinoma cell line lysate.FG pancreatic carcinoma cells treated with or without EGF (50ng/ml) for 15 minPPhospho-HER4-Y1188 Antibody was used at 1:750 in 3% BSA.Data and protocol kindly provided by Dr. Weis of Cheresh Lab, UCSD.)

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Phospho-HER4 (Y1188), Polyclonal Antibody (Cat# AAA284893)

Full Name

Phospho-HER4 (Y1188) Antibody

Gene Names

ERBB4; HER4; ALS19; p180erbB4

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

DB (Dot Blot) (Dot blot analysis of anti-Phospho-MeCP2-pS80 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

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Phospho-MeCP2 (S80), Polyclonal Antibody (Cat# AAA285391)

Full Name

Phospho-MeCP2 (S80) Antibody

Gene Names

MECP2; RS; RTS; RTT; PPMX; MRX16; MRX79; MRXSL; AUTSX3; MRXS13

Reactivity

Human (Predicted Reactivity: Monkey, Mouse, Rat)

Applications

Dot Blot

Purity

This antibody is purified through a protein A column, followed by peptide affinity purification.

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

WB (Western Blot) (Western blot analysis of lysates from SW480 cells, primary antibody was diluted at 1:1000, 4 degree C over night)

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MOB1, ELISA Kit (Cat# AAA319091)

Full Name

MOB1 (Phospho-Thr35) Colorimetric Cell-Based ELISA Kit

Gene Names

MOB1A; MOB1; MATS1; Mob4B; C2orf6; MOBK1B; MOBKL1B

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of extracts from NIH-3T3 cells, using Phospho-Catenin-beta (S552) Polyclonal Antibody.)

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Catenin-beta, ELISA Kit (Cat# AAA319161)

Full Name

Catenin-beta (Phospho-Ser552) Colorimetric Cell-Based ELISA Kit

Gene Names

CTNNB1; CTNNB; MRD19; armadillo

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from LOVO cells, using IL-2R alpha/CD25 (Phospho-Ser268) Antibody. The lane on the right is blocked with the phospho peptide.)

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IL-2Ralpha, ELISA Kit (Cat# AAA319172)

Full Name

IL-2Ralpha (Phospho-Ser268) Colorimetric Cell-Based ELISA Kit

Gene Names

IL2RA; CD25; IL2R; TCGFR; IDDM10

Reactivity

Human, Rat, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

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WB (Western Blot) (Western Blot analysis of various cells using Phospho-LIMK-2 (T505) Polyclonal Antibody)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).)

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LIMK-2, Polyclonal Antibody (Cat# AAA319461)

Full Name

LIMK-2 (Phospho-Thr505) Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$300/0.05 mL $420/0.1 mL $660/0.2 mL $1,285/2x0.2 mL $1,875/3x0.2 mL $2,430/4x0.2 mL $2,735/5x0.2 mL

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WB (Western Blot) (Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4 degree over night)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).)

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LDHA, Polyclonal Antibody (Cat# AAA319472)

Full Name

LDHA (Phospho-Tyr10) Antibody

Gene Names

LDHA; LDH1; LDHM; GSD11; PIG19

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Pricing

$300/0.05 mL $420/0.1 mL $660/0.2 mL $1,285/2x0.2 mL $1,875/3x0.2 mL $2,430/4x0.2 mL $2,735/5x0.2 mL

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WB (Western Blot) (Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4 degree over night)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).)

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MERIT40, Polyclonal Antibody (Cat# AAA319473)

Full Name

MERIT40 (Phospho-Ser29) Antibody

Gene Names

BABAM1; NBA1; HSPC142; MERIT40; C19orf62

Reactivity

Human, Rat, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Pricing

$300/0.05 mL $420/0.1 mL $660/0.2 mL $1,285/2x0.2 mL $1,875/3x0.2 mL $2,430/4x0.2 mL $2,735/5x0.2 mL

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WB (Western Blot) (Western blot analysis of Rac1(Phospho-Ser71) antibody in 293 cells lysates, cell treated or untreated with PMA 125ng/ml 30', 4 degree over night, secondary antibody(was diluted at 1:10000, 37 degree 1hour.)

IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human uterus. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).)

ELISA (Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Rac1(Phospho-Ser71) antibody)

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Rac1/CDC42, Polyclonal Antibody (Cat# AAA319481)

Full Name

Rac1/CDC42 (Phospho-Ser71) Antibody

Gene Names

RAC1; MIG5; Rac-1; TC-25; p21-Rac1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Pricing

$300/0.05 mL $420/0.1 mL $660/0.2 mL $1,285/2x0.2 mL $1,875/3x0.2 mL $2,430/4x0.2 mL $2,735/5x0.2 mL

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WB (Western Blot) (Western Blot analysis of Hela treated or untreated by LPS lysis, using primary antibody at 1:1000 dilution. Secondary antibody)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).)

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PPAR alpha, Polyclonal Antibody (Cat# AAA319489)

Full Name

PPAR alpha (Phospho-Ser21) Antibody

Gene Names

PPARA; PPAR; NR1C1; hPPAR; PPARalpha

Reactivity

Human, Rat, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Pricing

$300/0.05 mL $420/0.1 mL $660/0.2 mL $1,285/2x0.2 mL $1,875/3x0.2 mL $2,430/4x0.2 mL $2,735/5x0.2 mL

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WB (Western Blot) (Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4 degree over night)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).)

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Sox2, Polyclonal Antibody (Cat# AAA319497)

Full Name

Sox2 (Phospho-Ser250/Ser251) Antibody

Gene Names

SOX2; ANOP3; MCOPS3

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Pricing

$300/0.05 mL $420/0.1 mL $660/0.2 mL $1,285/2x0.2 mL $1,875/3x0.2 mL $2,430/4x0.2 mL $2,735/5x0.2 mL

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WB (Western Blot) (Western blot analysis of lysates from HCT116 cells, primary antibody was diluted at 1:1000, 4 degree over night)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).)

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Mcl-1, Polyclonal Antibody (Cat# AAA319499)

Full Name

Mcl-1 (Phospho-Ser64) Antibody

Gene Names

MCL1; TM; EAT; MCL1L; MCL1S; Mcl-1; BCL2L3; MCL1-ES; bcl2-L-3; mcl1/EAT

Reactivity

Human, Rat, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Pricing

$300/0.05 mL $420/0.1 mL $660/0.2 mL $1,285/2x0.2 mL $1,875/3x0.2 mL $2,430/4x0.2 mL $2,735/5x0.2 mL

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WB (Western Blot) (Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4 degree over night)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).)

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PRAS40, Polyclonal Antibody (Cat# AAA319501)

Full Name

PRAS40 (Phospho-Ser183) Antibody

Gene Names

AKT1S1; Lobe; PRAS40

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Pricing

$300/0.05 mL $420/0.1 mL $660/0.2 mL $1,285/2x0.2 mL $1,875/3x0.2 mL $2,430/4x0.2 mL $2,735/5x0.2 mL

WB (Western Blot) (Western Blot analysis of JK cells using Phospho-Bcl-2 (S70) Polyclonal Antibody)

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Bcl-2, ELISA Kit (Cat# AAA319291)

Full Name

Bcl-2 (Phospho-Ser70) Colorimetric Cell-Based ELISA Kit

Reactivity

Bovine

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

Application Data (The TFact c-Jun DNA-Binding ELISA kits detect active c-Jun in Hela Nuclear Extract. The Hela cells were grown 3 days in DMEM with 10% FBS and harvested for nuclear extract. The Hela cells were stimulated by UV (100J/M2) before harvest.)

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c-Jun, ELISA Kit (Cat# AAA315853)

Full Name

c-Jun (Phospho-Thr239) TFact DNA-Binding ELISA Kit

Gene Names

JUN; AP1; p39; AP-1; c-Jun

Reactivity

Human, Mouse, Rat

Pricing

$635/1 Kit $1,230/2x1 Kit $1,800/3x1 Kit $2,335/4x1 Kit $2,905/5x1 Kit

Application Data (The TFact c-Jun DNA-Binding ELISA kits detect active c-Jun in Hela Nuclear Extract. The Hela cells were grown 3 days in DMEM with 10% FBS and harvested for nuclear extract. The Hela cells were stimulated by UV (100J/M2) before harvest.)

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c-Jun, ELISA Kit (Cat# AAA315854)

Full Name

c-Jun (Phospho-Thr91) TFact DNA-Binding ELISA Kit

Gene Names

JUN; AP1; p39; AP-1; c-Jun

Reactivity

Human, Rat

Pricing

$635/1 Kit $1,230/2x1 Kit $1,800/3x1 Kit $2,335/4x1 Kit $2,905/5x1 Kit

Application Data (The TFact c-Jun DNA-Binding ELISA kits detect active c-Jun in Hela Nuclear Extract. The Hela cells were grown 3 days in DMEM with 10% FBS and harvested for nuclear extract. The Hela cells were stimulated by UV (100J/M2) before harvest.)

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c-Jun, ELISA Kit (Cat# AAA315855)

Full Name

c-Jun (Phospho-Thr93) TFact DNA-Binding ELISA Kit

Gene Names

JUN; AP1; p39; AP-1; c-Jun

Reactivity

Human, Mouse, Rat

Pricing

$635/1 Kit $1,230/2x1 Kit $1,800/3x1 Kit $2,335/4x1 Kit $2,905/5x1 Kit

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WB (Western Blot) (Western blot analysis of lysates from COS7 cells treated with EGF 200ng/ml 30', using POLR2A (Phospho-Ser1619) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using POLR2A (Phospho-Ser1619) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells treated with PMA 125ng/ml 30', using POLR2A (Phospho-Ser1619) Antibody. The picture on the right is blocked with the phospho peptide.)

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POLR2A, Polyclonal Antibody (Cat# AAA316113)

Full Name

POLR2A (Phospho-Ser1619) Antibody

Gene Names

POLR2A; RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from 293 cells treated with EGF 200ng/ml 30', using SEPARASE (Phospho-Ser801) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using SEPARASE (Phospho-Ser801) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HUVEC cells, using SEPARASE (Phospho-Ser801) Antibody. The picture on the right is blocked with the phospho peptide.)

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SEPARASE, Polyclonal Antibody (Cat# AAA316116)

Full Name

SEPARASE (Phospho-Ser801) Antibody

Gene Names

ESPL1; ESP1; SEPA

Reactivity

Human, Mouse

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from 293 cells treated with EGF 200ng/ml 30', using WNK1 (Phospho-Thr58) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using WNK1 (Phospho-Thr58) Antibody. The picture on the right is blocked with the phospho peptide.)

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WNK1, Polyclonal Antibody (Cat# AAA316123)

Full Name

WNK1 (Phospho-Thr58) Antibody

Gene Names

WNK1; KDP; PSK; p65; HSN2; HSAN2; PRKWNK1; PPP1R167

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from Jurkat cells treated with Insulin 0.01U/ml 15', using 4E-BP1 (Phospho-Ser64) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using 4E-BP1 (Phospho-Ser64) Antibody. The picture on the right is blocked with the phospho peptide.)

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4E-BP1, Polyclonal Antibody (Cat# AAA316124)

Full Name

4E-BP1 (Phospho-Ser64) Antibody

Gene Names

EIF4EBP1; BP-1; 4EBP1; 4E-BP1; PHAS-I

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from K562 cells treated with Insulin 0.01U/ml 15', using ACC1 (Phospho-Ser80) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ACC1 (Phospho-Ser80) Antibody. The picture on the right is blocked with the phospho peptide.)

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ACC1, Polyclonal Antibody (Cat# AAA316127)

Full Name

ACC1 (Phospho-Ser80) Antibody

Gene Names

ACACA; ACC; ACAC; ACC1; ACCA; ACACAD

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from mouse brain, using Akt (Phospho-Ser124) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Akt (Phospho-Ser124) Antibody. The picture on the right is blocked with the phospho peptide.)

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Akt, Polyclonal Antibody (Cat# AAA316129)

Full Name

Akt (Phospho-Ser124) Antibody

Gene Names

AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from Jurkat cells, using Chk2 (Phospho-Thr387) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Chk2 (Phospho-Thr387) Antibody. The picture on the right is blocked with the phospho peptide.)

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Chk2, Polyclonal Antibody (Cat# AAA316133)

Full Name

Chk2 (Phospho-Thr387) Antibody

Gene Names

CHEK2; CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from 293 cells treated with PBS 60', using Daxx (Phospho-Ser668) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human ovary, using Daxx (Phospho-Ser668) Antibody. The picture on the right is blocked with the phospho peptide.)

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Daxx, Polyclonal Antibody (Cat# AAA316135)

Full Name

Daxx (Phospho-Ser668) Antibody

Gene Names

DAXX; DAP6; EAP1; BING2

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HT29 cells treated with Calyculin A 50ng/ml 30', using FANCD2 (Phospho-Ser222) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using FANCD2 (Phospho-Ser222) Antibody. The picture on the right is blocked with the phospho peptide.)

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FANCD2, Polyclonal Antibody (Cat# AAA316140)

Full Name

FANCD2 (Phospho-Ser222) Antibody

Gene Names

FANCD2; FA4; FAD; FACD; FAD2; FA-D2; FANCD

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from K562 cells, using HSL (Phospho-Ser552) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using HSL (Phospho-Ser552) Antibody. The picture on the right is blocked with the phospho peptide.)

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HSL, Polyclonal Antibody (Cat# AAA316147)

Full Name

HSL (Phospho-Ser552) Antibody

Gene Names

LIPE; HSL; LHS; AOMS4; FPLD6

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from COS7 cells, using Ku80 (Phospho-Thr714) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using Ku80 (Phospho-Thr714) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells, using Ku80 (Phospho-Thr714) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

Ku80, Polyclonal Antibody (Cat# AAA316157)

Full Name

Ku80 (Phospho-Thr714) Antibody

Gene Names

XRCC5; KU80; KUB2; Ku86; NFIV; KARP1; KARP-1

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from 293 cells treated with PMA 125ng/ml 30', using ATPase (Phospho-Ser16) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using ATPase (Phospho-Ser16) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

ATPase, Polyclonal Antibody (Cat# AAA316161)

Full Name

ATPase (Phospho-Ser16) Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HeLa cells treated with EGF 200ng/ml 30', using Caldesmon (Phospho-Ser789) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Caldesmon (Phospho-Ser789) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells, using Caldesmon (Phospho-Ser789) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

Caldesmon, Polyclonal Antibody (Cat# AAA316164)

Full Name

Caldesmon (Phospho-Ser789) Antibody

Gene Names

CALD1; CDM; HCAD; LCAD; H-CAD; L-CAD; NAG22

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HeLa cells treated with EGF 200ng/ml 30', using CBL (Phospho-Tyr774) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using CBL (Phospho-Tyr774) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells, using CBL (Phospho-Tyr774) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

CBL, Polyclonal Antibody (Cat# AAA316166)

Full Name

CBL (Phospho-Tyr774) Antibody

Gene Names

CBL; CBL2; NSLL; C-CBL; RNF55; FRA11B

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from K562 cells treated with Na3VO4 0.3nM, using CBL (Phospho-Tyr700) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using CBL (Phospho-Tyr700) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HepG2 cells, using CBL (Phospho-Tyr700) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

CBL, Polyclonal Antibody (Cat# AAA316167)

Full Name

CBL (Phospho-Tyr700) Antibody

Gene Names

CBL; CBL2; NSLL; C-CBL; RNF55; FRA11B

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HepG2 cells treated with Forskolin 40nM 30', using CDK1/CDC2 (Phospho-Thr14) Antibody. The lane on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of COS7 cells, using CDK1/CDC2 (Phospho-Thr14) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

CDK1/CDC2, Polyclonal Antibody (Cat# AAA316169)

Full Name

CDK1/CDC2 (Phospho-Thr14) Antibody

Gene Names

CDK2; CDKN2; p33(CDK2)

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from HUVEC cells treated with Serum 20% 30', using EGFR (Phospho-Tyr1016) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using EGFR (Phospho-Tyr1016) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells, using EGFR (Phospho-Tyr1016) Antibody. The picture on the right is blocked with the phospho peptide.)

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EGFR, Polyclonal Antibody (Cat# AAA316170)

Full Name

EGFR (Phospho-Tyr1016) Antibody

Gene Names

EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from 293 cells, using Estrogen Receptor-alpha (Phospho-Tyr537) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using Estrogen Receptor-alpha (Phospho-Tyr537) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

Estrogen Receptor-alpha, Polyclonal Antibody (Cat# AAA316171)

Full Name

Estrogen Receptor-alpha (Phospho-Tyr537) Antibody

Gene Names

ESR1; ER; ESR; Era; ESRA; ESTRR; NR3A1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from HeLa cells treated with Serum 20% 15', using ETS1 (Phospho-Thr38) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ETS1 (Phospho-Thr38) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells, using ETS1 (Phospho-Thr38) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

ETS1, Polyclonal Antibody (Cat# AAA316173)

Full Name

ETS1 (Phospho-Thr38) Antibody

Gene Names

ETS1; p54; ETS-1; EWSR2; c-ets-1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from 293 cells treated with Insulin 0.01U/ml 15', using FGFR1 (Phospho-Tyr654) Antibody. The lane on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of COS7 cells, using FGFR1 (Phospho-Tyr654) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

FGFR1, Polyclonal Antibody (Cat# AAA316176)

Full Name

FGFR1 (Phospho-Tyr654) Antibody

Gene Names

FGFR1; CEK; FLG; HH2; OGD; ECCL; FLT2; KAL2; BFGFR; CD331; FGFBR; FLT-2; HBGFR; N-SAM; FGFR-1; HRTFDS; bFGF-R-1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from 293 cells treated with Heat shock, using IR (Phospho-Tyr1361) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using IR (Phospho-Tyr1361) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

IR, Polyclonal Antibody (Cat# AAA316184)

Full Name

IR (Phospho-Tyr1361) Antibody

Gene Names

INSR; HHF5; CD220

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from HepG2 cells treated with forskolin 40nM 30', using MEF2D (Phospho-Ser444) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using MEF2D (Phospho-Ser444) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

MEF2D, Polyclonal Antibody (Cat# AAA316193)

Full Name

MEF2D (Phospho-Ser444) Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from HeLa cells treated with Hu 2nM 24h, using MYB (Phospho-Ser12) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human heart, using MYB (Phospho-Ser12) Antibody. The picture on the right is blocked with the phospho peptide.)

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MYB, Polyclonal Antibody (Cat# AAA316194)

Full Name

MYB (Phospho-Ser12) Antibody

Gene Names

MYB; efg; Cmyb; c-myb; c-myb_CDS

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MYPT1 (Phospho-Thr696) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells, using MYPT1 (Phospho-Thr696) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

MYPT1, Polyclonal Antibody (Cat# AAA316195)

Full Name

MYPT1 (Phospho-Thr696) Antibody

Gene Names

PPP1R12A; MBS; M130; MYPT1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

Application Data (The TFact GATA1 (Phospho-Ser142) DNA-Binding ELISA detects active GATA1 (Phospho-Ser142) in Hela and Jurkat Nuclear Extract. The Hela and Jurkat cells were grown 3 days in DMEM and RPMI 1640 with 10% FBS and harvested for nuclear extract.)

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GATA1, ELISA Kit (Cat# AAA315912)

Full Name

GATA1 (Phospho-Ser142) TFact DNA-Binding ELISA Kit

Gene Names

GATA1; GF1; GF-1; NFE1; XLTT; ERYF1; NF-E1; XLANP; XLTDA; GATA-1

Reactivity

Human

Pricing

$635/1 Kit $1,230/2x1 Kit $1,800/3x1 Kit $2,335/4x1 Kit $2,905/5x1 Kit

What Are Phospho Antibodies?

Protein phosphorylation is a process where a phosphate group is added to certain amino acid residues of a protein – usually serine (S), threonine (T), or tyrosine (Y) - by enzymes called kinases. This process is integral in controlling cellular signaling, cellular growth, and other biological functions.

Our catalog includes a wide range of phospho-specific antibodies that can accurately detect this important marker. They perform strongly in widely-used laboratory applications such as Western blot, flow cytometry, immunohistochemistry, and immunofluorescence microscopy. We value your trust in us and are committed to providing top-quality products and services. All of our antibodies are guaranteed to work for the applications and species indicated on our website & associated product pages.

What Are The Key Applications of Phospho Antibodies?

1. Western Blotting

One of the first steps a researcher can take in utilizing these phospho-specific antibodies, is to check if the antibody works using a technique referred to as “Western blot”. For those unfamiliar, Western Blot aids in showing whether the protein that the antibody recognizes is appearing at the correct/expected size. These phospho-specific antibodies should also be able to detect changes in the target protein’s phosphorylation (on/off state) when cells are stimulated in certain ways.

2. Staining of Fixed Cells (Immunocytochemistry)

Another routine use of these phospho-specific antibodies, is to test if the antibody is able to demonstrate similar performance when used on fixed cells (intact cells that have been preserved) as it did in the Western blot tests. It is an important aspect in many cases to confirm that the antibody works in actual intact cell samples. Ideally, the method used for cellular fixation should be the same as what is used in pathology labs (like using 10% formalin). To check if the antibody works well in tissue sections (FFPE), researchers will often test it on fixed cells that are processed similar to tissue samples.

3. Specificity Tests Using Peptides

In order to make sure that the antibody is only binding to the right target:

• Laboratory technicians will mix the antibody with phospho-peptides (short segments of the protein containing the phosphate group modification).


• If the antibody signal disappears, it is confirmation that it is binding to the correct phosphorylated location.


• A more robust test is to use both the phosphorylated and non-phosphorylated (dephosphorylated) versions of the protein. The antibody should react only with the phosphorylated one.


• Another method sometimes utilized is to treat the sample with an enzyme, such as alkaline phosphatase, that specifically removes phosphate groups. If the antibody signal disappears after this, it also confirms specificity.

4. Genetic Confirmation

As a final step, scientists can genetically manipulate the nucleotide sequence and alter the target protein by removing the exact site where phosphorylation happens. If the antibody no longer appears to detect the modified protein, it is strong evidence supporting the antibody being specific for that phosphorylated site.

Why Buy Phospho Antibodies Through Us?

• The production laboratory adheres to strict and consistent protocols prior to releasing any of these phospho-specific antibodies:


• Standard methods and proper controls in all tests to ensure high quality.


• These antibodies are tested and validated in different cell types and species.


• High quality control criterion to ensure each batch is consistent, so you will obtain reliable results every time.

FAQ

1. What Are Phospho-Specific Antibodies?

Phospho-specific antibodies are made to detect proteins only when they have a phosphate group linked to a specific amino acid residue. This empowers scientists understand if a protein is "turned on" or active, based on its phosphorylation state.

2. How to Detect Phosphorylated Proteins in a Western Blot?

To find out if a protein is phosphorylated using Western blot:

• Use a phospho-specific antibody that binds only to the phosphorylated form of the protein.


• You can also use a “regular” antibody for the same amino acid sequence of the protein that the phospho-specific antibody is binding to (but in this case, this antibody will not bind if there is a phosphate group present) in order to compare how much of it is phosphorylated versus how much is non-phosphorylated (or “total” protein, if the “normal” antibody’s epitopes are non-phospho-site-specific).

3. How to Choose the Best Antibody?

Here are some simple tips to help you pick the right antibody:

• Know your target


• Match your sample characteristics


• Confirm the intended use is appropriate


• Check “host” and “type”


• Check the “quality” of the presented data/images


• Appraise whether the available validation meets your needs