Phospho Antibodies

Phospho-specific antibodies’ typical purpose is to enable researchers to detect changes in proteins. They will exclusively bind to the amino acid sequence on a protein that has been phosphorylated (which is both a physical & chemical change) and do not bind to the same amino acid sequence on said protein if it lacks said phosphorylation. This aids in being able to clearly see and understand the data produced from this particular protein modification.

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WB (Western Blot) (Western blot analysis of lysates from Jurkat cells treated with EGF 200ng/ml 15', using PKC thet (Phospho-Ser695) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PKC thet (Phospho-Ser695) Antibody. The picture on the right is blocked with the phospho peptide.)

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PKC thet, Polyclonal Antibody (Cat# AAA316304)

Full Name

PKC thet (Phospho-Ser695) Antibody

Gene Names

PRKCQ; PRKCT; nPKC-theta

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HUVEC cells treated with Anisomycin 25ug/ml 30', using ATF2 (Phospho-Ser480) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using ATF2 (Phospho-Ser480) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells, using ATF2 (Phospho-Ser480) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

ATF2, Polyclonal Antibody (Cat# AAA316313)

Full Name

ATF2 (Phospho-Ser480) Antibody

Gene Names

ATF2; HB16; CREB2; TREB7; CREB-2; CRE-BP1

Reactivity

Human, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from COS7 cells treated with TNF-a 20ng/ml+Calyculin A 50nM 5', using BAD (Phospho-Ser91/128) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using BAD (Phospho-Ser91/128) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

BAD, Polyclonal Antibody (Cat# AAA316314)

Full Name

BAD (Phospho-Ser91/128) Antibody

Gene Names

BAD; BBC2; BCL2L8

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HUVEC cells treated with UV 15', using C/EBP-epsilon (Phospho-Thr74) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using C/EBP-epsilon (Phospho-Thr74) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells, using C/EBP-epsilon (Phospho-Thr74) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

C/EBP-epsilon, Polyclonal Antibody (Cat# AAA316317)

Full Name

C/EBP-epsilon (Phospho-Thr74) Antibody

Gene Names

CEBPE; CRP1; C/EBP-epsilon

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from LOVO cells, using Calcium Sensing Receptor (Phospho-Thr888) Antibody. The lane on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells, using Calcium Sensing Receptor (Phospho-Thr888) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

Calcium Sensing Receptor, Polyclonal Antibody (Cat# AAA316318)

Full Name

Calcium Sensing Receptor (Phospho-Thr888) Antibody

Gene Names

CASR; CAR; FHH; FIH; HHC; EIG8; HHC1; NSHPT; PCAR1; hCasR; GPRC2A; HYPOC1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from Jurkat cells treated with Insulin 0.01U/ml 15', using Calmodulin (Phospho-Thr79+Ser81) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using Calmodulin (Phospho-Thr79+Ser81) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HepG2 cells, using Calmodulin (Phospho-Thr79+Ser81) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

Calmodulin, Polyclonal Antibody (Cat# AAA316319)

Full Name

Calmodulin (Phospho-Thr79+Ser81) Antibody

Gene Names

CALM1; caM; CAMI; PHKD; CPVT4; DD132; LQT14; CALML2

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from COS7 cells treated with EGF 200ng/ml 30', using CD18/ITGB2 (Phospho-Thr758) Antibody. The lane on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HepG2 cells, using CD18/ITGB2 (Phospho-Thr758) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

CD18/ITGB2, Polyclonal Antibody (Cat# AAA316326)

Full Name

CD18/ITGB2 (Phospho-Thr758) Antibody

Gene Names

ITGB2; LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from Jurkat cells treated with PMA 125ng/ml 30', using CHOP (Phospho-Ser30) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using CHOP (Phospho-Ser30) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells, using CHOP (Phospho-Ser30) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

CHOP, Polyclonal Antibody (Cat# AAA316336)

Full Name

CHOP (Phospho-Ser30) Antibody

Gene Names

DDIT3; CHOP; CEBPZ; CHOP10; CHOP-10; GADD153; C/EBPzeta

Reactivity

Human, Mouse

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HUVEC cells, using CKII-beta (Phospho-Ser209) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using CKII-beta (Phospho-Ser209) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

CKII-beta, Polyclonal Antibody (Cat# AAA316338)

Full Name

CKII-beta (Phospho-Ser209) Antibody

Gene Names

CSNK2B; G5A; CK2B; CK2N; Ckb1; Ckb2; CSK2B

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HepG2 cells, using c-Met (Phospho-Tyr1003) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using c-Met (Phospho-Tyr1003) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HepG2 cells, using c-Met (Phospho-Tyr1003) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

c-Met, Polyclonal Antibody (Cat# AAA316339)

Full Name

c-Met (Phospho-Tyr1003) Antibody

Gene Names

MET; HGFR; AUTS9; RCCP2; c-Met; DFNB97

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HepG2 cells, using CSFR (Phospho-Tyr561) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using CSFR (Phospho-Tyr561) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

CSFR, Polyclonal Antibody (Cat# AAA316341)

Full Name

CSFR (Phospho-Tyr561) Antibody

Gene Names

CSF1R; FMS; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HepG2 cells, using EPHB1/2 (Phospho-Tyr594/604) Antibody. The lane on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HUVEC cells, using EPHB1/2 (Phospho-Tyr594/604) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

EPHB1/2, Polyclonal Antibody (Cat# AAA316357)

Full Name

EPHB1/2 (Phospho-Tyr594/604) Antibody

Gene Names

EPHB1; ELK; NET; Hek6; EPHT2

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from COLO205 cells treated with serum 20% 15', using Elk3 (Phospho-Ser357) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using Elk3 (Phospho-Ser357) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells, using Elk3 (Phospho-Ser357) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

Elk3, Polyclonal Antibody (Cat# AAA316361)

Full Name

Elk3 (Phospho-Ser357) Antibody

Gene Names

ELK3; ERP; NET; SAP2; SAP-2

Reactivity

Human, Mouse

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HeLa cells treated with calyculinA 50ng/ml 30', using UBF (Phospho-Ser484) Antibody.)

ELISA (Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using UBF (Phospho-Ser484) Antibody)

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UBF, Polyclonal Antibody (Cat# AAA318405)

Full Name

UBF (Phospho-Ser484) Antibody

Gene Names

UBTF; UBF; UBF1; UBF2; UBF-1; CONDBA; NOR-90

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$345/0.05 mL $450/0.1 mL $880/2x0.1 mL $1,285/3x0.1 mL $1,660/4x0.1 mL $1,775/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT6(Phospho-Tyr641) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from Hela cells untreated or treated with IL-4 using STAT6(Phospho-Tyr641) Antibody.)

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STAT6, Polyclonal Antibody (Cat# AAA243059)

Full Name

Phospho-STAT6 (Tyr641) Antibody

Gene Names

STAT6; STAT6B; STAT6C; D12S1644; IL-4-STAT

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATF4(Phospho-Ser245) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from Hela cells untreated or treated with UV using ATF4(Phospho-Ser245) Antibody.)

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ATF4, Polyclonal Antibody (Cat# AAA243062)

Full Name

Phospho-ATF4 (Ser245) Antibody

Gene Names

ATF4; CREB2; TXREB; CREB-2; TAXREB67

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using MARCKS(Phospho-Ser162) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from 293 cells untreated or treated with PMA using MARCKS(Phospho-Ser162) Antibody.)

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MARCKS, Polyclonal Antibody (Cat# AAA243133)

Full Name

Phospho-MARCKS (Ser162) Antibody

Gene Names

MARCKS; MACS; 80K-L; PKCSL; PRKCSL

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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WB (Western Blot) (Western blot analysis of Phospho-Tau (S198) expression in mouse hippocampus cell lysate.)

WB (Western Blot) (All lanes use the Antibody at 1:2W dilution for 1 hour at room temperature.)

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Tau, Monoclonal Antibody (Cat# AAA128104)

Full Name

Anti-Phospho-Tau (S198) Rabbit Monoclonal Antibody

Gene Names

MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17

Reactivity

Human, Mouse, Rat

Applications

Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

Affinity-chromatography

Pricing

$215/0.03 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,005/5x0.1 mL

WB (Western Blot) (Western Blot analysis of Hela, 293T cells with Phospho-GR (Ser226) Polyclonal Antibody at dilution of 1:500)

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GR, Polyclonal Antibody (Cat# AAA172317)

Full Name

Phospho-GR (S226) Polyclonal Antibody

Gene Names

Nr3c1; GRL

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purified

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

WB (Western Blot) (Western blot of HeLa lysate showing specific labeling of the ~95k IR protein phosphorylated at Thr1160 (Control). Immunolabeling is completely eliminated by treatment with lambda-phosphatase (?-Ptase).)

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Thr1160 Insulin Receptor, Polyclonal Antibody (Cat# AAA72828)

Full Name

Anti-Phospho-Thr1160 Insulin Receptor

Gene Names

INSR; HHF5; CD220

Applications

Western Blot

Purity

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Pricing

$500/0.1 mL $975/2x0.1 mL $1,430/3x0.1 mL $1,855/4x0.1 mL $2,060/5x0.1 mL

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Application Data

IHC (Immunohistochemistry)

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MEK2, Polyclonal Antibody (Cat# AAA47792)

Full Name

MEK2 (Phospho-Thr394)

Gene Names

MAP2K2; CFC4; MEK2; MKK2; MAPKK2; PRKMK2

Applications

Immunohistochemistry, Western Blot

Pricing

$475/0.1 mL $925/2x0.1 mL $1,355/3x0.1 mL $1,755/4x0.1 mL $1,980/5x0.1 mL

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Application Data

IHC (Immunohiostchemistry)

IHC (Immunohistochemistry)

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NFkB-p65, Polyclonal Antibody (Cat# AAA47795)

Full Name

NFkB-p65 (Phospho-Ser276)

Gene Names

RELA; p65; NFKB3

Applications

Immunohistochemistry, Western Blot

Pricing

$475/0.1 mL $925/2x0.1 mL $1,355/3x0.1 mL $1,755/4x0.1 mL $1,980/5x0.1 mL

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IHC (Immunohiostchemistry)

IF (Immunofluorescence)

Compare (5 max)

c-Jun, Polyclonal Antibody (Cat# AAA47808)

Full Name

c-Jun (Phospho-Ser79)

Gene Names

JUN; AP1; AP-1; c-Jun

Applications

Immunofluorescence, Immunohistochemistry

Pricing

$475/0.1 mL $925/2x0.1 mL $1,355/3x0.1 mL $1,755/4x0.1 mL $1,980/5x0.1 mL

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Application Data

IHC (Immunohistochemistry)

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JunD, Polyclonal Antibody (Cat# AAA47810)

Full Name

JunD (Phospho-Ser255)

Gene Names

JUND; AP-1

Applications

Immunohistochemistry, Immunoblot

Pricing

$475/0.1 mL $925/2x0.1 mL $1,355/3x0.1 mL $1,755/4x0.1 mL $1,980/5x0.1 mL

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Application Data

IHC (Immunohistochemistry)

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Myc, Polyclonal Antibody (Cat# AAA47816)

Full Name

Myc (phospho-Thr58)

Gene Names

MYC; MRTL; MYCC; c-Myc; bHLHe39

Applications

Immunohistochemistry, Immunoblot

Pricing

$475/0.1 mL $925/2x0.1 mL $1,355/3x0.1 mL $1,755/4x0.1 mL $1,980/5x0.1 mL

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Application Data

IHC (Immunohistochemistry)

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Myc, Polyclonal Antibody (Cat# AAA47818)

Full Name

Myc (phospho-Ser373)

Gene Names

MYC; MRTL; MYCC; c-Myc; bHLHe39

Applications

Immunohistochemistry, Immunoblot

Pricing

$475/0.1 mL $925/2x0.1 mL $1,355/3x0.1 mL $1,755/4x0.1 mL $1,980/5x0.1 mL

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Application Data

IHC (Immunohistochemistry)

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STAT5A, Polyclonal Antibody (Cat# AAA47828)

Full Name

STAT5A (phospho-Ser780)

Gene Names

STAT5A; MGF; STAT5

Applications

Immunohistochemistry, Immunoblot

Pricing

$475/0.1 mL $925/2x0.1 mL $1,355/3x0.1 mL $1,755/4x0.1 mL $1,980/5x0.1 mL

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Application Data

IHC (Immunohiostchemistry)

IF (Immunofluorescence)

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PTEN, Polyclonal Antibody (Cat# AAA47835)

Full Name

PTEN (Phospho-Ser380/Thr382/Thr83)

Gene Names

PTEN; BZS; DEC; CWS1; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del

Applications

Immunofluorescence, Immunohistochemistry, Immunoblot

Pricing

$475/0.1 mL $925/2x0.1 mL $1,355/3x0.1 mL $1,755/4x0.1 mL $1,980/5x0.1 mL

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Application Data

IHC (Immunohiostchemistry)

IF (Immunofluorescence)

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PTEN, Polyclonal Antibody (Cat# AAA47837)

Full Name

PTEN (Phospho-Ser370)

Gene Names

PTEN; BZS; DEC; CWS1; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del

Applications

Immunofluorescence, Immunohistochemistry, Immunoblot

Pricing

$475/0.1 mL $925/2x0.1 mL $1,355/3x0.1 mL $1,755/4x0.1 mL $1,980/5x0.1 mL

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Application Data

IHC (Immunohistochemistry)

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Estrogen Receptor-a, Polyclonal Antibody (Cat# AAA47843)

Full Name

Estrogen Receptor-a (Phospho-Ser104)

Gene Names

ESR1; ER; ESR; Era; ESRA; ESTRR; NR3A1

Applications

Immunohistochemistry, Immunoblot

Pricing

$475/0.1 mL $925/2x0.1 mL $1,355/3x0.1 mL $1,755/4x0.1 mL $1,980/5x0.1 mL

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Application Data

IHC (Immunohiostchemistry)

IF (Immunofluorescence)

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Progesterone Receptor, Polyclonal Antibody (Cat# AAA47847)

Full Name

Progesterone Receptor (Phospho-Ser190)

Gene Names

PGR; PR; NR3C3

Applications

Immunohistochemistry, Immunofluorescence, Immunoblot

Pricing

$475/0.1 mL $925/2x0.1 mL $1,355/3x0.1 mL $1,755/4x0.1 mL $1,980/5x0.1 mL

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Application Data

Application Data

IF (Immunofluorescence)

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HER2, Polyclonal Antibody (Cat# AAA47849)

Full Name

HER2 (Phospho-Tyr1221/Try1222)

Gene Names

ERBB2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu

Applications

Immunofluorescence, Immunoblot

Pricing

$475/0.1 mL $925/2x0.1 mL $1,355/3x0.1 mL $1,755/4x0.1 mL $1,980/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MAPKAPK2 (Phospho-Thr334) antibody. Western blot analysis of extracts from NIH/3T3 cells, using MAPKAPK2 (Phospho-Thr334) antibody.)

Application Data (P-peptide-+)

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MAPKAPK2, Antibody (Cat# AAA109003)

Full Name

MAPKAPK2 (Phospho-Thr334) Antibody

Gene Names

MAPKAPK2; MK2; MK-2; MAPKAP-K2

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

WB (Western Blot)

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Phospho-MAP3K12, Positive Control (Cat# AAA75982)

Full Name

Phospho-MAP3K12 Antibody Positive Control

Gene Names

MAP3K12; DLK; MUK; ZPK; ZPKP1; MEKK12

Applications

Western Blot

Pricing

$395/5 Applications $765/2x5 Applications $1,120/3x5 Applications $1,450/4x5 Applications $1,560/5x5 Applications

Standard Curve (Sample)

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Phospho exiracellular signal regulated kinase, ELISA Kit (Cat# AAA87209)

Full Name

Human Phospho exiracellular signal regulated kinase ELISA Kit

Reactivity

Human

Pricing

$595/48-Strip-Wells $845/96-Strip-Wells $1,640/2x96-Strip-Wells $3,715/5x96-Strip-Wells $7,005/10x96-Strip-Wells

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WB (Western Blot) (Detection of human Phospho MCM2 (Ser41) by western blot. Samples: Whole cell lysate (30 ug) from HeLa cells that were either untreated or treated with alkaline phosphatase (AP). Antibodies: Affinity purified rabbit anti-phospho MCM2 (Ser41) antibody AAA210771 used at 0.1 ug/ml. After probing with the phospho-specific antibodies, rabbit anti-MCM2 antibody was used at 0.2 ug/ml to show total MCM2. Detection: Chemiluminescence with a 1 minute exposure.)

WB (Western Blot) (Detection of human Phospho MCM2 (S41) by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Phospho MCM2 (S41) antibody AAA210771 (lot AAA210771-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)

IHC (Immunohistochemistry) (Detection of human and mouse MCM2 (Ser41) by immunohistochemistry. Sample: FFPE section of human laryngeal squamous cell carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-MCM2 (Ser41) (Cat. No. AAA210771 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)

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MCM2, Polyclonal Antibody (Cat# AAA210771)

Full Name

Rabbit anti-Phospho MCM2 (S41) Antibody, Affinity Purified

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purified

Pricing

$255/0.01 mg $635/0.1 mg $940/5x0.01 mg $1,230/2x0.1 mg $1,800/3x0.1 mg $2,335/4x0.1 mg $2,630/5x0.1 mg

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).)

WB (Western Blot) (Western Blot analysis of Hela treated or untreated by LPS lysis, using primary antibody at 1:1000 dilution. Secondary antibody)

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Rad50, Polyclonal Antibody (Cat# AAA318655)

Full Name

Rad50 (Phospho-Ser635) Rabbit Polyclonal Antibody

Gene Names

RAD50; NBSLD; RAD502; hRad50

Reactivity

Human, Rat, Mouse

Applications

Western Blot

Purity

The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from Jurkat cells treated with starved 24h, using TIF-IA (Phospho-Ser649) Antibody.)

ELISA (Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using TIF-IA (Phospho-Ser649) Antibody)

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TIF-IA, Polyclonal Antibody (Cat# AAA318398)

Full Name

TIF-IA (Phospho-Ser649) Antibody

Gene Names

RRN3; TIFIA; A-270G1.2

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$345/0.05 mL $450/0.1 mL $880/2x0.1 mL $1,285/3x0.1 mL $1,660/4x0.1 mL $1,775/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HepG2 cells, using IRS-1 (Phospho-Ser312) Antibody.)

ELISA (Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using IRS-1 (Phospho-Ser312) Antibody)

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IRS-1, Polyclonal Antibody (Cat# AAA318334)

Full Name

IRS-1 (Phospho-Ser312) Antibody

Gene Names

IRS1; HIRS-1

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$345/0.05 mL $450/0.1 mL $880/2x0.1 mL $1,285/3x0.1 mL $1,660/4x0.1 mL $1,775/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from 293 cells, using Stathmin 1 (Phospho-Ser15) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Stathmin 1 (Phospho-Ser15) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of COS7 cells, using Stathmin 1 (Phospho-Ser15) Antibody. The picture on the right is blocked with the phospho peptide.)

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Stathmin 1, Polyclonal Antibody (Cat# AAA316585)

Full Name

Stathmin 1 (Phospho-Ser15) Antibody

Gene Names

STMN1; Lag; SMN; OP18; PP17; PP19; PR22; LAP18; C1orf215

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HeLa cells treated with nocodazole, using Stathmin 1 (Phospho-Ser37) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Stathmin 1 (Phospho-Ser37) Antibody. The picture on the right is blocked with the phospho peptide.)

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Stathmin 1, Polyclonal Antibody (Cat# AAA316587)

Full Name

Stathmin 1 (Phospho-Ser37) Antibody

Gene Names

STMN1; Lag; SMN; OP18; PP17; PP19; PR22; LAP18; C1orf215

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from 293 cells treated with serum 10% 15', using Tau (Phospho-Ser356) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using Tau (Phospho-Ser356) Antibody. The picture on the right is blocked with the phospho peptide.)

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Tau, Polyclonal Antibody (Cat# AAA316588)

Full Name

Tau (Phospho-Ser356) Antibody

Gene Names

MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from mouse brain, using Tau (Phospho-Ser422) Antibody. The lane on the left is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using Tau (Phospho-Ser422) Antibody. The picture on the right is blocked with the phospho peptide.)

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Tau, Polyclonal Antibody (Cat# AAA316589)

Full Name

Tau (Phospho-Ser422) Antibody

Gene Names

MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from NIH/3T3 cells treated with forskolin 40 muM 30', using VASP (Phospho-Ser157) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human tonsil, using VASP (Phospho-Ser157) Antibody. The picture on the right is blocked with the phospho peptide.)

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VASP, Polyclonal Antibody (Cat# AAA316590)

Full Name

VASP (Phospho-Ser157) Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from RAW264.7 cells, using VASP (Phospho-Ser238) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human tonsil, using VASP (Phospho-Ser238) Antibody. The picture on the right is blocked with the phospho peptide.)

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VASP, Polyclonal Antibody (Cat# AAA316591)

Full Name

VASP (Phospho-Ser238) Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HepG2 cells treated with Na3VO4 0.3nM 40', using VEGFR2 (Phospho-Tyr1214) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using VEGFR2 (Phospho-Tyr1214) Antibody. The picture on the right is blocked with the phospho peptide.)

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VEGFR2, Polyclonal Antibody (Cat# AAA316593)

Full Name

VEGFR2 (Phospho-Tyr1214) Antibody

Gene Names

KDR; FLK1; CD309; VEGFR; VEGFR2

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from SK-OV3 cells, using VEGFR2 (Phospho-Tyr951) Antibody. The lane on the left is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using VEGFR2 (Phospho-Tyr951) Antibody. The picture on the right is blocked with the phospho peptide.)

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VEGFR2, Polyclonal Antibody (Cat# AAA316594)

Full Name

VEGFR2 (Phospho-Tyr951) Antibody

Gene Names

KDR; FLK1; CD309; VEGFR; VEGFR2

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from COS7 cells treated with UV 15', using Catenin-beta (Phospho-Tyr489) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using Catenin-beta (Phospho-Tyr489) Antibody. The picture on the right is blocked with the phospho peptide.)

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Catenin-beta, Polyclonal Antibody (Cat# AAA316596)

Full Name

Catenin-beta (Phospho-Tyr489) Antibody

Gene Names

CTNNB1; EVR7; CTNNB; MRD19; armadillo

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

What Are Phospho Antibodies?

Protein phosphorylation is a process where a phosphate group is added to certain amino acid residues of a protein – usually serine (S), threonine (T), or tyrosine (Y) - by enzymes called kinases. This process is integral in controlling cellular signaling, cellular growth, and other biological functions.

Our catalog includes a wide range of phospho-specific antibodies that can accurately detect this important marker. They perform strongly in widely-used laboratory applications such as Western blot, flow cytometry, immunohistochemistry, and immunofluorescence microscopy. We value your trust in us and are committed to providing top-quality products and services. All of our antibodies are guaranteed to work for the applications and species indicated on our website & associated product pages.

What Are The Key Applications of Phospho Antibodies?

1. Western Blotting

One of the first steps a researcher can take in utilizing these phospho-specific antibodies, is to check if the antibody works using a technique referred to as “Western blot”. For those unfamiliar, Western Blot aids in showing whether the protein that the antibody recognizes is appearing at the correct/expected size. These phospho-specific antibodies should also be able to detect changes in the target protein’s phosphorylation (on/off state) when cells are stimulated in certain ways.

2. Staining of Fixed Cells (Immunocytochemistry)

Another routine use of these phospho-specific antibodies, is to test if the antibody is able to demonstrate similar performance when used on fixed cells (intact cells that have been preserved) as it did in the Western blot tests. It is an important aspect in many cases to confirm that the antibody works in actual intact cell samples. Ideally, the method used for cellular fixation should be the same as what is used in pathology labs (like using 10% formalin). To check if the antibody works well in tissue sections (FFPE), researchers will often test it on fixed cells that are processed similar to tissue samples.

3. Specificity Tests Using Peptides

In order to make sure that the antibody is only binding to the right target:

• Laboratory technicians will mix the antibody with phospho-peptides (short segments of the protein containing the phosphate group modification).


• If the antibody signal disappears, it is confirmation that it is binding to the correct phosphorylated location.


• A more robust test is to use both the phosphorylated and non-phosphorylated (dephosphorylated) versions of the protein. The antibody should react only with the phosphorylated one.


• Another method sometimes utilized is to treat the sample with an enzyme, such as alkaline phosphatase, that specifically removes phosphate groups. If the antibody signal disappears after this, it also confirms specificity.

4. Genetic Confirmation

As a final step, scientists can genetically manipulate the nucleotide sequence and alter the target protein by removing the exact site where phosphorylation happens. If the antibody no longer appears to detect the modified protein, it is strong evidence supporting the antibody being specific for that phosphorylated site.

Why Buy Phospho Antibodies Through Us?

• The production laboratory adheres to strict and consistent protocols prior to releasing any of these phospho-specific antibodies:


• Standard methods and proper controls in all tests to ensure high quality.


• These antibodies are tested and validated in different cell types and species.


• High quality control criterion to ensure each batch is consistent, so you will obtain reliable results every time.

FAQ

1. What Are Phospho-Specific Antibodies?

Phospho-specific antibodies are made to detect proteins only when they have a phosphate group linked to a specific amino acid residue. This empowers scientists understand if a protein is "turned on" or active, based on its phosphorylation state.

2. How to Detect Phosphorylated Proteins in a Western Blot?

To find out if a protein is phosphorylated using Western blot:

• Use a phospho-specific antibody that binds only to the phosphorylated form of the protein.


• You can also use a “regular” antibody for the same amino acid sequence of the protein that the phospho-specific antibody is binding to (but in this case, this antibody will not bind if there is a phosphate group present) in order to compare how much of it is phosphorylated versus how much is non-phosphorylated (or “total” protein, if the “normal” antibody’s epitopes are non-phospho-site-specific).

3. How to Choose the Best Antibody?

Here are some simple tips to help you pick the right antibody:

• Know your target


• Match your sample characteristics


• Confirm the intended use is appropriate


• Check “host” and “type”


• Check the “quality” of the presented data/images


• Appraise whether the available validation meets your needs