Phospho Antibodies

Phospho-specific antibodies’ typical purpose is to enable researchers to detect changes in proteins. They will exclusively bind to the amino acid sequence on a protein that has been phosphorylated (which is both a physical & chemical change) and do not bind to the same amino acid sequence on said protein if it lacks said phosphorylation. This aids in being able to clearly see and understand the data produced from this particular protein modification.

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WB (Western Blot) (Western blot analysis of EGFR (arrow) in Hela cell lysates, either induced (Lane 1) or noninduced with EGF (Lane 2).)

DB (Dot Blot) (Dot blot analysis of Phospho-EGFR-Y998 Antibody and EGFR Non Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

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Phospho-EGFR (Y998), Polyclonal Antibody (Cat# AAA288513)

Full Name

Phospho-EGFR (Y998) Antibody

Gene Names

EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2

Reactivity

Human (Predicted Reactivity: Mouse)

Applications

Western Blot, Dot Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

DB (Dot Blot) (Dot blot analysis of anti-Phospho-MEK1 Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

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Bi-Phospho-MEK1 (S218/222), Polyclonal Antibody (Cat# AAA288138)

Full Name

Bi-Phospho-MEK1 (S218/222) Antibody

Gene Names

MAP2K1; CFC3; MEK1; MKK1; MAPKK1; PRKMK1

Reactivity

Human (Predicted Reactivity: Mouse, Rat, Rabbit, Hamster, Chicken, Xenopus, C Elegans, Drosophila)

Applications

Immunohistochemistry, Dot Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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IF (Immunofluorescence) (Immunofluorescent analysis of RSK1/2/3 (phospho-Thr573/570/577) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of RSK1/2/3 (phospho-Thr573/570/577) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot) (Western blot analysis of RSK1/2/3 (phospho-Thr573/570/577) expression in Hela, HepG2 whole cell lysates.)

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RSK1/2/3 (phospho-Thr573/570/577), Polyclonal Antibody (Cat# AAA310517)

Full Name

RSK1/2/3 (phospho-Thr573/570/577) Antibody

Gene Names

RPS6KA1; RSK; HU-1; RSK1; p90Rsk; MAPKAPK1A

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Immunogen affinity chromatography

Pricing

$340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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DB (Dot Blot) (Dot blot analysis of anti-mPDHE1A-S232 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)

WB (Western Blot) (Western blot analysis of lysates from 293 cell line, untreated or treated with CIP, 100ng/ml, using Phospho-PDHE1A(S232) Antibody(upper) or Tubulin (lower).)

WB (Western Blot) (Western blot analysis of lysates from C6 cell line, untreated or treated with CIP, 100ng/ml, using Phospho-PDHE1A(S232) Antibody(upper) or Tubulin (lower).)

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Phospho-PDHE1A (S232), Polyclonal Antibody (Cat# AAA285709)

Full Name

Phospho-PDHE1A (S232) Antibody

Gene Names

PDHA1; PDHA; PDHAD; PHE1A; PDHCE1A

Reactivity

Human (Predicted Reactivity: Bovine, Monkey, Mouse, Pig, Rat)

Applications

Western Blot, Dot Blot

Purity

This antibody is purified through a protein A column, followed by peptide affinity purification.

Pricing

$245/0.05 mL $485/0.2 mL $945/2x0.2 mL $1,385/3x0.2 mL $1,795/4x0.2 mL $2,040/5x0.2 mL

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DB (Dot Blot) (Dot blot analysis of anti-EGFR-pS1070 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

WB (Western Blot) (Western blot analysis of extracts from A431 cell, untreated or treated with EGF, using phospho-EGFR-pS1070 (left) or ErBB2 antibody (right).)

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Phospho-EGFR (S1070), Polyclonal Antibody (Cat# AAA285783)

Full Name

Phospho-EGFR (S1070) Antibody

Gene Names

EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2

Reactivity

Human

Applications

Western Blot, Dot Blot

Purity

This antibody is purified through a protein A column, followed by peptide affinity purification.

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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DB (Dot Blot) (Dot blot analysis of Phospho-ERBB2-Y1112 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)

WB (Western Blot) (Western blot analysis of extracts from A431 cells, untreated or treated with EGF at 100ng/ml, using phospho-ErBB2 (Y1112) (left) or ErBB2 antibody (right).)

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Phospho-ErbB2 (Y1112), Polyclonal Antibody (Cat# AAA285456)

Full Name

Phospho-ErbB2 (Y1112) Antibody

Gene Names

ERBB2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu

Reactivity

Human

Applications

Western Blot, Dot Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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WB (Western Blot) (Western blot analysis of extracts from 293 cells (Lane 2), HeLa cells (Lane 3) and HepG2 cells (Lane 4), using AML1 (Phospho-Ser435) Antibody. The lane on the left is treated with synthesized peptide.)

WB (Western Blot) (Western blot analysis of extracts from HepG2 cells, treated with PMA (125ng/ml, 30mins), using AML1 (Phospho-Ser435) antibody.)

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AML1 (Phospho-Ser435), Polyclonal Antibody (Cat# AAA289468)

Full Name

AML1 (Phospho-Ser435) Antibody

Gene Names

RUNX1; AML1; CBFA2; EVI-1; AMLCR1; PEBP2aB; AML1-EVI-1

Applications

Western Blot

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

IHC (Immunohistochemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

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Phospho-ERK3-S189, Polyclonal Antibody (Cat# AAA289488)

Full Name

Phospho-ERK3-S189 Antibody

Gene Names

MAPK6; ERK3; PRKM6; p97MAPK; HsT17250

Reactivity

Human
Predicted Reactivity: Mouse, Rat, Chicken

Applications

Immunohistochemistry

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of Filamin A (phospho-Ser2152)/Filamin B (phospho-Ser2107) in paraffin-embedded Human Cholangiocarcinoma using Filamin A (phospho-Ser2152)/Filamin B (phospho-Ser2107) Rabbit mAb at dilution 1/50)

WB (Western Blot) (Western blot detection of Filamin A (phospho-Ser2152)/Filamin B (phospho-Ser2107) in Hela,A549,U2OS,C6 using Filamin A (phospho-Ser2152)/Filamin B (phospho-Ser2107) Rabbit mAb(1:1000 diluted))

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Filamin A/B, Monoclonal Antibody (Cat# AAA314500)

Full Name

Phospho-Filamin A/B (Ser2152/Ser2107) Rabbit mAb

Gene Names

FLNB; AOI; FH1; SCT; TAP; LRS1; TABP; FLN-B; FLN1L; ABP-278; ABP-280

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Affinity Purified

Pricing

$290/0.05 mL $365/0.1 mL $705/2x0.1 mL $1,030/3x0.1 mL $1,335/4x0.1 mL $1,425/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of MCM2 (phospho-Ser40) in paraffin-embedded Human tonsil using MCM2 (phospho-Ser40) Rabbit mAb at dilution 1/50)

WB (Western Blot) (Western blot detection of phospho-MCM2(Ser40) in Hela,A549,MCF-7,293T,C2C12 cell lysates using phospho-MCM2(Ser40) Rabbit mAb(1:1000 diluted).Predicted band size:102kDa.Observed band size:125kDa.)

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MCM2, Monoclonal Antibody (Cat# AAA314503)

Full Name

Phospho-MCM2 (Ser40) Rabbit mAb

Gene Names

MCM2; BM28; CCNL1; CDCL1; cdc19; D3S3194; MITOTIN

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity Purified

Pricing

$290/0.05 mL $365/0.1 mL $705/2x0.1 mL $1,030/3x0.1 mL $1,335/4x0.1 mL $1,425/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human colon, using Phospho-DNA PKcs (S2056) Antibody.)

WB (Western Blot) (Western blot analysis of Phospho-DNA PKcs (Ser2056) expression in alkaline treated Jurkat cell lysate.)

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DNA PKcs, Monoclonal Recombinant Antibody (Cat# AAA314844)

Full Name

DNA PKcs (Phospho-Ser2056) Rabbit mAb

Gene Names

PRKDC; HYRC; p350; DNAPK; DNPK1; HYRC1; XRCC7; DNA-PKcs

Reactivity

Human

Applications

Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Affinity-chromatography

Pricing

$290/0.05 mL $365/0.1 mL $705/2x0.1 mL $1,030/3x0.1 mL $1,335/4x0.1 mL $1,425/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human colon, using Phospho-PKR (T446) Antibody.)

WB (Western Blot) (Western blot analysis of PKR phosphorylation expression in HeLa cell lysate treated with Calyculin A and TNF-alpha.)

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PKR, Monoclonal Recombinant Antibody (Cat# AAA314848)

Full Name

PKR (Phospho-Thr446) Rabbit mAb

Gene Names

EIF2AK2; PKR; PRKR; EIF2AK1

Reactivity

Human

Applications

Chromatin immunoprecipitation, Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

Affinity-chromatography

Pricing

$290/0.05 mL $365/0.1 mL $705/2x0.1 mL $1,030/3x0.1 mL $1,335/4x0.1 mL $1,425/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded mouse spleen, using Phospho-Retinoblastoma (S807) Antibody .)

WB (Western Blot) (Western blot analysis of Phospho-Retinoblastoma (S807) expression in K562 cell lysate.)

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Retinoblastoma, Monoclonal Recombinant Antibody (Cat# AAA314853)

Full Name

Retinoblastoma (Phospho-Ser807) Rabbit mAb

Gene Names

RB1; RB; pRb; OSRC; pp110; p105-Rb

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Affinity-chromatography

Pricing

$290/0.05 mL $365/0.1 mL $705/2x0.1 mL $1,030/3x0.1 mL $1,335/4x0.1 mL $1,425/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human bladder cancer, using Phospho-GATA3 (S308) Antibody.)

WB (Western Blot) (Western blot analysis of Phospho-GATA3 (S308) Jurkat cell lysate treated with Bromo-cAMP.)

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GATA3, Monoclonal Recombinant Antibody (Cat# AAA314855)

Full Name

GATA3 (Phospho-Ser308) Rabbit mAb

Gene Names

GATA3; HDR; HDRS

Reactivity

Human

Applications

Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

Affinity-chromatography

Pricing

$290/0.05 mL $365/0.1 mL $705/2x0.1 mL $1,030/3x0.1 mL $1,335/4x0.1 mL $1,425/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescent analysis of NIH/3T3 cells treated with Anisomycin, using Phospho-JNK1/2/3 (T183+T183+T221) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast, using Phospho-JNK1/2/3 (T183+T183+T221) Antibody.)

WB (Western Blot) (Western blot analysis of JNK1/2/3 phosphorylation expression in NIH/3T3 cell lysate treated with Anisomycin.)

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JNK1/2/3, Monoclonal Recombinant Antibody (Cat# AAA314857)

Full Name

JNK1/2/3 (Phospho-Thr183+Thr183+Thr221) Rabbit mAb

Gene Names

MAPK8; JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Affinity-chromatography

Pricing

$290/0.05 mL $365/0.1 mL $705/2x0.1 mL $1,030/3x0.1 mL $1,335/4x0.1 mL $1,425/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain cancer, using Phospho-TrkB (Y817) Antibody.)

WB (Western Blot) (Western blot analysis of Phospho-TrkB (Y817) expression in SH-SY5Y cell lysate treated with BDNF.)

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TrkB, Monoclonal Recombinant Antibody (Cat# AAA314860)

Full Name

TrkB (Phospho-Tyr817) Rabbit mAb

Gene Names

NTRK2; TRKB; trk-B; GP145-TrkB

Reactivity

Human, Mouse, Rat

Applications

Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Affinity-chromatography

Pricing

$290/0.05 mL $365/0.1 mL $705/2x0.1 mL $1,030/3x0.1 mL $1,335/4x0.1 mL $1,425/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded rat stomach, using Phospho-EGFR (Y1173) Antibody.)

WB (Western Blot) (Western blot analysis of Phospho-EGFR (Y1173) expression in A431 cell lysate treated with EGF.)

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EGFR, Monoclonal Recombinant Antibody (Cat# AAA314861)

Full Name

EGFR (Phospho-Tyr1173) Rabbit mAb

Gene Names

EGFR; ERBB; HER1; mENA; ERBB1; PIG61

Reactivity

Human

Applications

Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Affinity-chromatography

Pricing

$290/0.05 mL $365/0.1 mL $705/2x0.1 mL $1,030/3x0.1 mL $1,335/4x0.1 mL $1,425/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescent analysis of MCF7 cells treated with EGF, using Phospho-ER alpha (S118) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human kidney, using Phospho-ER alpha (S118) Antibody.)

WB (Western Blot) (Western blot analysis of Phospho-ER alpha (S118) expression in (1) MCF7 cell lysate; (2) MCF7 cell lysate treated with b-Estradiol and EGF.)

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ER alpha, Monoclonal Recombinant Antibody (Cat# AAA314862)

Full Name

ER alpha (Phospho-Ser118) Rabbit mAb

Gene Names

ESR1; ER; ESR; Era; ESRA; NR3A1

Reactivity

Human

Applications

Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Affinity-chromatography

Pricing

$290/0.05 mL $365/0.1 mL $705/2x0.1 mL $1,030/3x0.1 mL $1,335/4x0.1 mL $1,425/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human lung cancer, using Phospho-STAT1 (S727) Antibody.)

WB (Western Blot) (Western blot analysis of Phospho-STAT1 (S727) expression in HeLa cell lysate treated with etoposide.)

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STAT1, Monoclonal Recombinant Antibody (Cat# AAA314867)

Full Name

STAT1 (Phospho-Ser727) Rabbit mAb

Gene Names

STAT1; CANDF7; ISGF-3; STAT91

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-chromatography

Pricing

$290/0.05 mL $365/0.1 mL $705/2x0.1 mL $1,030/3x0.1 mL $1,335/4x0.1 mL $1,425/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human bladder cancer, using Phospho-CDK2 (Y15) Antibody.)

WB (Western Blot) (Western blot analysis of Phospho-CDK2 (Y15) expression in (1) HeLa treated with AP; (2) HeLa cell lysate.)

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CDK2, Monoclonal Recombinant Antibody (Cat# AAA314869)

Full Name

CDK2 (Phospho-Tyr15) Rabbit mAb

Gene Names

CDK2; p33(CDK2)

Reactivity

Human, Mouse, Rat

Applications

Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

Affinity-chromatography

Pricing

$290/0.05 mL $365/0.1 mL $705/2x0.1 mL $1,030/3x0.1 mL $1,335/4x0.1 mL $1,425/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescent analysis of HeLa cells treated with IFN-alpha, using Phospho-STAT3 (Y705) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human pancreas, using Phospho-STAT3 (Y705) Antibody.)

WB (Western Blot) (Western blot analysis of Phospho-STAT3 (Tyr705) expression in (1) HeLa cell lysate; (2) HeLa cell lysate treated with IFN-a.)

Compare (5 max)

STAT3, Monoclonal Recombinant Antibody (Cat# AAA314871)

Full Name

STAT3 (Phospho-Tyr705) Rabbit mAb

Gene Names

STAT3; APRF; HIES

Reactivity

Human, Mouse, Rat

Applications

Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Affinity-chromatography

Pricing

$290/0.05 mL $365/0.1 mL $705/2x0.1 mL $1,030/3x0.1 mL $1,335/4x0.1 mL $1,425/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescent analysis of Jurkat cells treated with Pervanadate, using Phospho-JAK2 (Y1007 + Y1008) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human cervix cancer, using Phospho-JAK2 (Y1007 + Y1008) Antibody.)

WB (Western Blot) (Western blot analysis of JAK2 phosphorylation expression in Jurkat cell lysates treated with Pervanadate.)

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JAK2, Monoclonal Recombinant Antibody (Cat# AAA314874)

Full Name

JAK2 (Phospho-Tyr1007/Tyr1008) Rabbit mAb

Gene Names

JAK2; JTK10; THCYT3

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Affinity-chromatography

Pricing

$290/0.05 mL $365/0.1 mL $705/2x0.1 mL $1,030/3x0.1 mL $1,335/4x0.1 mL $1,425/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human gastric carcinoma, using Phospho-Creb (S133) Antibody.)

WB (Western Blot) (Western blot analysis of Phospho-Creb(Ser133) expression in HeLa cell lysates treated with Calyculin A.)

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Creb, Monoclonal Recombinant Antibody (Cat# AAA314839)

Full Name

Creb (Phospho-Ser133) Rabbit mAb

Gene Names

CREB1; CREB

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Affinity-chromatography

Pricing

$290/0.05 mL $365/0.1 mL $705/2x0.1 mL $1,030/3x0.1 mL $1,335/4x0.1 mL $1,425/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil, using Phospho-Histone H3 (T3) Antibody.)

WB (Western Blot) (Western blot analysis of Phospho-Histone H3 (Thr3) in HeLa cell lysates treated with FBS + Calyculin A.)

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Histone H3, Monoclonal Recombinant Antibody (Cat# AAA314840)

Full Name

Histone H3 (Phospho-Thr3) Rabbit mAb

Gene Names

H3F3A; H3F3; H3.3A

Reactivity

Human

Applications

Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Affinity-chromatography

Pricing

$290/0.05 mL $365/0.1 mL $705/2x0.1 mL $1,030/3x0.1 mL $1,335/4x0.1 mL $1,425/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human liver, using Phospho-ATM (S1981) Antibody.)

WB (Western Blot) (Western blot analysis of Phospho-ATM (Ser1981) in (1) HEK293 cell lysate; (2) HEK293 cell lysate treated with Doxorubicin.)

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ATM, Monoclonal Recombinant Antibody (Cat# AAA314841)

Full Name

ATM (Phospho-Ser1981) Rabbit mAb

Gene Names

ATM; AT1; ATA; ATC; ATD; ATE; ATDC; TEL1; TELO1

Reactivity

Human

Applications

Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Affinity-chromatography

Pricing

$290/0.05 mL $365/0.1 mL $705/2x0.1 mL $1,030/3x0.1 mL $1,335/4x0.1 mL $1,425/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from A431 cells, using SHP-2 (Phospho-Tyr542) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using SHP-2 (Phospho-Tyr542) Antibody. The picture on the right is blocked with the phospho peptide.)

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SHP-2, Polyclonal Antibody (Cat# AAA316076)

Full Name

SHP-2 (Phospho-Tyr542) Antibody

Gene Names

PTPN11; CFC; NS1; JMML; SHP2; BPTP3; PTP2C; METCDS; PTP-1D; SH-PTP2; SH-PTP3

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HeLa cells treated with Heat shock, using GR (Phospho-Ser211) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using GR (Phospho-Ser211) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

GR, Polyclonal Antibody (Cat# AAA316104)

Full Name

GR (Phospho-Ser211) Antibody

Gene Names

NR3C1; GR; GCR; GRL; GCCR; GCRST

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from COS7 cells, using MDM2 (Phospho-Ser166) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MDM2 (Phospho-Ser166) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells, using MDM2 (Phospho-Ser166) Antibody. The picture on the right is blocked with the phospho peptide.)

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MDM2, Polyclonal Antibody (Cat# AAA316192)

Full Name

MDM2 (Phospho-Ser166) Antibody

Gene Names

MDM2; HDMX; hdm2; ACTFS

Reactivity

Human, Mouse

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

Application Data (The TFact c-Jun DNA-Binding ELISA kits detect active c-Jun in Hela Nuclear Extract. The Hela cells were grown 3 days in DMEM with 10% FBS and harvested for nuclear extract. The Hela cells were stimulated by UV (100J/M2) before harvest.)

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c-Jun, ELISA Kit (Cat# AAA315852)

Full Name

c-Jun (Phospho-Ser243) TFact DNA-Binding ELISA Kit

Gene Names

JUN; AP1; p39; AP-1; c-Jun

Reactivity

Human, Mouse, Rat

Pricing

$735/1 Kit $1,435/2x1 Kit $2,095/3x1 Kit $2,720/4x1 Kit $3,355/5x1 Kit

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WB (Western Blot) (Western blot analysis of lysates from HepG2 cells, using ETK (Phospho-Tyr40) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human skin, using ETK (Phospho-Tyr40) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells, using ETK (Phospho-Tyr40) Antibody. The picture on the right is blocked with the phospho peptide.)

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ETK, Polyclonal Antibody (Cat# AAA316256)

Full Name

ETK (Phospho-Tyr40) Antibody

Gene Names

BMX; ETK; PSCTK2; PSCTK3

Reactivity

Human, Mouse

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using Gab1(Phospho-Tyr627) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from 293 cells untreated or treated with EGF using Gab1(Phospho-Tyr627) Antibody.)

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Gab1, Polyclonal Antibody (Cat# AAA304753)

Full Name

Gab1 (Phospho-Tyr627) Antibody

Reactivity

Human, Mouse

Applications

Immunofluorescence, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Elk-1(Phospho-Ser383) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from HT29 cells untreated or treated with UV using Elk-1(Phospho-Ser383) Antibody.)

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Elk-1, Polyclonal Antibody (Cat# AAA306167)

Full Name

Elk-1 (Phospho-Ser383) Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using MDM4 (Phospho-Ser367) antibody. The picture on the right is treated with the synthesized peptide.)

WB (Western Blot) (Western blot analysis of extracts from HeLa cells, treated with calyculinA (50ng/ml, 30mins), using MDM4 (Phospho-Ser367) antibody. The lane on the right is treated with the synthesized peptide.)

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MDM4, Polyclonal Antibody (Cat# AAA304240)

Full Name

MDM4 (Phospho-Ser367) Antibody

Gene Names

MDM4; HDMX; MDMX; MRP1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of various cells using Phospho-IRS-1 (S794) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of Rat cells using Phospho-IRS-1 (S794) Polyclonal Antibody)

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IRS-1, Polyclonal Antibody (Cat# AAA305781)

Full Name

IRS-1 (Phospho-Ser794) Polyclonal Antibody

Gene Names

IRS1; HIRS-1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using SEK1/MKK4(Phospho-Ser80) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SEK1/MKK4(Phospho-Ser80) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from HepG2 cells untreated or treated with EGF using SEK1/MKK4(Phospho-Ser80) Antibody.)

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SEK1/MKK4, Polyclonal Antibody (Cat# AAA305933)

Full Name

SEK1/MKK4 (Phospho-Ser80) Antibody

Gene Names

MAP2K4; JNKK; MEK4; MKK4; SEK1; SKK1; JNKK1; SERK1; MAPKK4; PRKMK4; SAPKK1; SAPKK-1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATF4(Phospho-Ser245) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from Hela cells untreated or treated with UV using ATF4(Phospho-Ser245) Antibody.)

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ATF4, Polyclonal Antibody (Cat# AAA303561)

Full Name

ATF4 (Phospho-Ser245) Antibody

Gene Names

ATF4; CREB2; TXREB; CREB-2; TAXREB67

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HepG2 cells treated with PMA 125ng/ml 30', using HER2 (Phospho-Tyr1248) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using HER2 (Phospho-Tyr1248) Antibody. The picture on the right is blocked with the phospho peptide.)

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HER2, Polyclonal Antibody (Cat# AAA316506)

Full Name

HER2 (Phospho-Tyr1248) Antibody

Gene Names

ERBB2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HeLa cells treated with EGF, using p21 Cip1 (Phospho-Thr145) Antibody. The lane on the left is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using p21 Cip1 (Phospho-Thr145) Antibody. The picture on the right is blocked with the phospho peptide.)

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p21 Cip1, Polyclonal Antibody (Cat# AAA316553)

Full Name

p21 Cip1 (Phospho-Thr145) Antibody

Gene Names

CDKN1A; P21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; p21CIP1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from NIH/3T3 cells treated with EGF 200ng/ml 30', using p70 S6 Kinase (Phospho-Thr421) Antibody. The lane on the left is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using p70 S6 Kinase (Phospho-Thr421) Antibody. The picture on the right is blocked with the phospho peptide.)

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p70 S6 Kinase, Polyclonal Antibody (Cat# AAA316560)

Full Name

p70 S6 Kinase (Phospho-Thr421) Antibody

Gene Names

RPS6KB1; S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from 293 cells and NIH/3T3 cells, using SEK1/MKK4 (Phospho-Ser80) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using SEK1/MKK4 (Phospho-Ser80) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

SEK1/MKK4, Polyclonal Antibody (Cat# AAA316572)

Full Name

SEK1/MKK4 (Phospho-Ser80) Antibody

Gene Names

MAP2K4; JNKK; MEK4; MKK4; SEK1; SKK1; JNKK1; SERK1; MAPKK4; PRKMK4; SAPKK1; SAPKK-1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HUVEC cells treated with Forskolin 40nM 30', using Nibrin (Phospho-Ser278) Antibody. The lane on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells, using Nibrin (Phospho-Ser278) Antibody. The picture on the right is blocked with the phospho peptide.)

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Nibrin, Polyclonal Antibody (Cat# AAA316440)

Full Name

Nibrin (Phospho-Ser278) Antibody

Gene Names

NBN; ATV; NBS; P95; NBS1; AT-V1; AT-V2

Reactivity

Human

Applications

Immunofluorescence, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from K562 cells treated with PMA 200ng/ml 10', using Connexin 43 (Phospho-Ser367) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Connexin 43 (Phospho-Ser367) Antibody. The picture on the right is blocked with the phospho peptide.)

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Connexin 43, Polyclonal Antibody (Cat# AAA316477)

Full Name

Connexin 43 (Phospho-Ser367) Antibody

Gene Names

GJA1; HSS; CMDR; CX43; EKVP; GJAL; ODDD; AVSD3; EKVP3; HLHS1; PPKCA

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from 293 cells treated with serum, using AFX (Phospho-Ser197) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using AFX (Phospho-Ser197) Antibody. The picture on the right is blocked with the phospho peptide.)

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AFX, Polyclonal Antibody (Cat# AAA316496)

Full Name

AFX (Phospho-Ser197) Antibody

Gene Names

FOXO4; AFX; AFX1; MLLT7

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from mouse brain, using GluR2 (Phospho-Ser880) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using GluR2 (Phospho-Ser880) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

GluR2, Polyclonal Antibody (Cat# AAA316500)

Full Name

GluR2 (Phospho-Ser880) Antibody

Gene Names

GRIA2; GLUR2; GLURB; GluA2; HBGR2; GluR-K2

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from Jurkat cells treated with starved 24h, using FOXO4 (Phospho-Ser262) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using FOXO4 (Phospho-Ser262) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

FOXO4, Polyclonal Antibody (Cat# AAA316598)

Full Name

FOXO4 (Phospho-Ser262) Antibody

Gene Names

FOXO4; AFX; AFX1; MLLT7

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

IHC (Immunohistochemistry) (Immunohistochemistry analysis of Formalin-fixed, paraffin-embedded Human testis using GCN2 (Phospho-Thr899) Antibody (1:100).)

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GCN2, Polyclonal Antibody (Cat# AAA316613)

Full Name

GCN2 (Phospho-Thr899) Antibody

Gene Names

EIF2AK4; GCN2; PVOD2

Reactivity

Human, Mouse

Applications

Immunohistochemistry

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from COLO205 cells, using STK39 (Phospho-Ser311) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using STK39 (Phospho-Ser311) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

STK39, Polyclonal Antibody (Cat# AAA316629)

Full Name

STK39 (Phospho-Ser311) Antibody

Gene Names

STK39; DCHT; PASK; SPAK

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from RAW264.7 cells treated with UV 5', using PSEN1 (Phospho-Ser357) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using PSEN1 (Phospho-Ser357) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

PSEN1, Polyclonal Antibody (Cat# AAA316660)

Full Name

PSEN1 (Phospho-Ser357) Antibody

Gene Names

PSEN1; AD3; FAD; PS1; PS-1; S182; ACNINV3

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

What Are Phospho Antibodies?

Protein phosphorylation is a process where a phosphate group is added to certain amino acid residues of a protein – usually serine (S), threonine (T), or tyrosine (Y) - by enzymes called kinases. This process is integral in controlling cellular signaling, cellular growth, and other biological functions.

Our catalog includes a wide range of phospho-specific antibodies that can accurately detect this important marker. They perform strongly in widely-used laboratory applications such as Western blot, flow cytometry, immunohistochemistry, and immunofluorescence microscopy. We value your trust in us and are committed to providing top-quality products and services. All of our antibodies are guaranteed to work for the applications and species indicated on our website & associated product pages.

What Are The Key Applications of Phospho Antibodies?

1. Western Blotting

One of the first steps a researcher can take in utilizing these phospho-specific antibodies, is to check if the antibody works using a technique referred to as “Western blot”. For those unfamiliar, Western Blot aids in showing whether the protein that the antibody recognizes is appearing at the correct/expected size. These phospho-specific antibodies should also be able to detect changes in the target protein’s phosphorylation (on/off state) when cells are stimulated in certain ways.

2. Staining of Fixed Cells (Immunocytochemistry)

Another routine use of these phospho-specific antibodies, is to test if the antibody is able to demonstrate similar performance when used on fixed cells (intact cells that have been preserved) as it did in the Western blot tests. It is an important aspect in many cases to confirm that the antibody works in actual intact cell samples. Ideally, the method used for cellular fixation should be the same as what is used in pathology labs (like using 10% formalin). To check if the antibody works well in tissue sections (FFPE), researchers will often test it on fixed cells that are processed similar to tissue samples.

3. Specificity Tests Using Peptides

In order to make sure that the antibody is only binding to the right target:

• Laboratory technicians will mix the antibody with phospho-peptides (short segments of the protein containing the phosphate group modification).


• If the antibody signal disappears, it is confirmation that it is binding to the correct phosphorylated location.


• A more robust test is to use both the phosphorylated and non-phosphorylated (dephosphorylated) versions of the protein. The antibody should react only with the phosphorylated one.


• Another method sometimes utilized is to treat the sample with an enzyme, such as alkaline phosphatase, that specifically removes phosphate groups. If the antibody signal disappears after this, it also confirms specificity.

4. Genetic Confirmation

As a final step, scientists can genetically manipulate the nucleotide sequence and alter the target protein by removing the exact site where phosphorylation happens. If the antibody no longer appears to detect the modified protein, it is strong evidence supporting the antibody being specific for that phosphorylated site.

Why Buy Phospho Antibodies Through Us?

• The production laboratory adheres to strict and consistent protocols prior to releasing any of these phospho-specific antibodies:


• Standard methods and proper controls in all tests to ensure high quality.


• These antibodies are tested and validated in different cell types and species.


• High quality control criterion to ensure each batch is consistent, so you will obtain reliable results every time.

FAQ

1. What Are Phospho-Specific Antibodies?

Phospho-specific antibodies are made to detect proteins only when they have a phosphate group linked to a specific amino acid residue. This empowers scientists understand if a protein is "turned on" or active, based on its phosphorylation state.

2. How to Detect Phosphorylated Proteins in a Western Blot?

To find out if a protein is phosphorylated using Western blot:

• Use a phospho-specific antibody that binds only to the phosphorylated form of the protein.


• You can also use a “regular” antibody for the same amino acid sequence of the protein that the phospho-specific antibody is binding to (but in this case, this antibody will not bind if there is a phosphate group present) in order to compare how much of it is phosphorylated versus how much is non-phosphorylated (or “total” protein, if the “normal” antibody’s epitopes are non-phospho-site-specific).

3. How to Choose the Best Antibody?

Here are some simple tips to help you pick the right antibody:

• Know your target


• Match your sample characteristics


• Confirm the intended use is appropriate


• Check “host” and “type”


• Check the “quality” of the presented data/images


• Appraise whether the available validation meets your needs