Phospho Antibodies

Phospho-specific antibodies’ typical purpose is to enable researchers to detect changes in proteins. They will exclusively bind to the amino acid sequence on a protein that has been phosphorylated (which is both a physical & chemical change) and do not bind to the same amino acid sequence on said protein if it lacks said phosphorylation. This aids in being able to clearly see and understand the data produced from this particular protein modification.

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tumor tissue using Bcr(Phospho-Tyr177) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from K562 cells untreated or treated with H2O2 using Bcr(Phospho-Tyr177) Antibody.)

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BCR, Polyclonal Antibody (Cat# AAA243113)

Full Name

Phospho-BCR (Tyr177) Antibody

Gene Names

BCR; ALL; CML; PHL; BCR1; D22S11; D22S662

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts from HT29 cells, treated with PMA or calf intestinal phosphatase (CIP), using Cdc25B (Phospho-Ser149) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from 3T3 cells untreated(lane 1) or treated with Hydroxyurea(lane 2) using Cdc25B(Phospho-Ser149) Antibody.)

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Cdc25b, Polyclonal Antibody (Cat# AAA243163)

Full Name

Phospho-Cdc25b (Ser149) Antibody

Gene Names

Cdc25b; AI604853

Reactivity

Mouse, Rat

Applications

Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were Purified by affinity-chromatography using epitope-specific peptide.

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed HeLa cells using EPHA2/3/4 (Phospho-Tyr588/596) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from HepG2 cells using EPHA2/3/4 (Phospho-Tyr588/596) Antibody.The lane on the right is treated with the antigen-specific peptide.)

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EPHA2/EPHA3/EPHA4, Polyclonal Antibody (Cat# AAA243198)

Full Name

Phospho-EPHA2/EPHA3/EPHA4 (Tyr588/596) Antibody

Gene Names

EPHA2; ECK; CTPA; ARCC2; CTPP1; CTRCT6

Reactivity

Human, Mouse

Applications

Immunofluorescence, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using c-Jun (Phospho-Ser63) antibody (left)or the same antibody preincubated with blocking peptide (right).)

WB (Western Blot) (Western blot analysis of extracts from HeLa cells using c-Jun (Phospho-Ser63) Antibody.The lane on the right is treated with the antigen-specific peptide.)

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JUN, Polyclonal Antibody (Cat# AAA243203)

Full Name

Phospho-JUN (Ser63) Antibody

Gene Names

JUN; AP1; AP-1; c-Jun

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human brain tissue using MER/SKY (Phospho-Tyr749/681) antibody (left)or the same antibody preincubated with blocking peptide (right).)

WB (Western Blot) (Western blot analysis of extracts from 3T3 cells treated with EGF using MER/SKY (Phospho-Tyr749/681) Antibody.The lane on the right is treated with the antigen-specific peptide.)

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MERTK/TYRO3, Polyclonal Antibody (Cat# AAA243208)

Full Name

Phospho-MERTK/TYRO3 (Tyr749/681) Antibody

Gene Names

MERTK; MER; RP38; c-mer

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HT29 cells, using SNAI1 (Phospho-Ser246) Antibody. The lane on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HUVEC cells, using SNAI1 (Phospho-Ser246) Antibody. The picture on the right is blocked with the phospho peptide.)

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SNAI1, Polyclonal Antibody (Cat# AAA316444)

Full Name

SNAI1 (Phospho-Ser246) Antibody

Gene Names

SNAI1; SNA; SNAH; SNAIL; SLUGH2; SNAIL1; dJ710H13.1

Reactivity

Human, Mouse

Applications

Immunofluorescence, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from 293 cells treated with Forskolin, using BAD (Phospho-Ser155) Antibody. The lane on the left is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using BAD (Phospho-Ser155) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

BAD, Polyclonal Antibody (Cat# AAA316463)

Full Name

BAD (Phospho-Ser155) Antibody

Gene Names

BAD; BBC2; BCL2L8

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from 293 cells, using Cortactin (Phospho-Tyr466) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Cortactin (Phospho-Tyr466) Antibody. The picture on the right is blocked with the phospho peptide.)

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Cortactin, Polyclonal Antibody (Cat# AAA316478)

Full Name

Cortactin (Phospho-Tyr466) Antibody

Gene Names

Cttn; Ems1; 1110020L01Rik

Reactivity

Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from NIH/3T3 cells, using HDAC8 (Phospho-Ser39) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using HDAC8 (Phospho-Ser39) Antibody. The picture on the right is blocked with the phospho peptide.)

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HDAC8, Polyclonal Antibody (Cat# AAA316504)

Full Name

HDAC8 (Phospho-Ser39) Antibody

Gene Names

HDAC8; HD8; WTS; RPD3; CDA07; CDLS5; MRXS6; HDACL1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HeLa and Jurkat cells, using NF-kappaB p65 (Phospho-Ser529) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using NF-kappaB p65 (Phospho-Ser529) Antibody. The picture on the right is blocked with the phospho peptide.)

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NF-kappaB p65, Polyclonal Antibody (Cat# AAA316540)

Full Name

NF-kappaB p65 (Phospho-Ser529) Antibody

Gene Names

RELA; p65; NFKB3

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from MDA-MB-435 cells treated with TNF-alpha, using NF-kappaB p100/p52 (Phospho-Ser869) Antibody. The lane on the left is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using NF-kappaB p100/p52 (Phospho-Ser869) Antibody. The picture on the right is blocked with the phospho peptide.)

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NF-kappaB p100/p52, Polyclonal Antibody (Cat# AAA316543)

Full Name

NF-kappaB p100/p52 (Phospho-Ser869) Antibody

Gene Names

NFKB2; p52; p100; H2TF1; LYT10; CVID10; LYT-10; NF-kB2; p49/p100

Reactivity

Human, Mouse, Rat

Applications

Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from LOVO cells and RAW264.7 cells, using RelB (Phospho-Ser552) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using RelB (Phospho-Ser552) Antibody. The picture on the right is blocked with the phospho peptide.)

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RelB, Polyclonal Antibody (Cat# AAA316571)

Full Name

RelB (Phospho-Ser552) Antibody

Gene Names

RELB; IREL; I-REL; IMD53; REL-B

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HeLa cells, using STAT3 (Phospho-Tyr705) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using STAT3 (Phospho-Tyr705) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

STAT3, Polyclonal Antibody (Cat# AAA316580)

Full Name

STAT3 (Phospho-Tyr705) Antibody

Gene Names

STAT3; APRF; HIES; ADMIO; ADMIO1

Reactivity

Human, Mouse, Rat

Applications

Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from 293 cells treated with Insulin 0.01U/ml 30', using Tyrosine Hydroxylase (Phospho-Ser19) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using Tyrosine Hydroxylase (Phospho-Ser19) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HUVEC cells, using Tyrosine Hydroxylase (Phospho-Ser19) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

Tyrosine Hydroxylase, Polyclonal Antibody (Cat# AAA316085)

Full Name

Tyrosine Hydroxylase (Phospho-Ser19) Antibody

Gene Names

TH; TYH; DYT14; DYT5b

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from NIH/3T3 cells treated with Serum 10% 30', using eEF2 (Phospho-Thr56) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using eEF2 (Phospho-Thr56) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HUVEC cells, using eEF2 (Phospho-Thr56) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

eEF2, Polyclonal Antibody (Cat# AAA316101)

Full Name

eEF2 (Phospho-Thr56) Antibody

Gene Names

EEF2; EF2; EF-2; EEF-2; SCA26

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from LOVO cells treated with Serum 10% 15', using SRF (Phospho-Ser99) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using SRF (Phospho-Ser99) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

SRF, Polyclonal Antibody (Cat# AAA316118)

Full Name

SRF (Phospho-Ser99) Antibody

Gene Names

SRF; MCM1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HepG2 cells, using WASP (Phospho-Tyr290) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using WASP (Phospho-Tyr290) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

WASP, Polyclonal Antibody (Cat# AAA316237)

Full Name

WASP (Phospho-Tyr290) Antibody

Gene Names

WAS; THC; IMD2; SCNX; THC1; WASP; WASPA

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HepG2 cells treated with EGF 200ng/ml 30', using ACK1 (Phospho-Tyr284) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ACK1 (Phospho-Tyr284) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells, using ACK1 (Phospho-Tyr284) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

ACK1, Polyclonal Antibody (Cat# AAA316241)

Full Name

ACK1 (Phospho-Tyr284) Antibody

Gene Names

TNK2; ACK; ACK1; ACK-1; p21cdc42Hs

Reactivity

Human, Mouse

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HepG2 cells treated with EGF 200ng/ml 30' and A549 cells treated with PMA 125ng/ml 30', using p130 Cas (Phospho-Tyr249) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using p130 Cas (Phospho-Tyr249) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

p130 Cas, Polyclonal Antibody (Cat# AAA316270)

Full Name

p130 Cas (Phospho-Tyr249) Antibody

Gene Names

BCAR1; CAS; CAS1; CASS1; CRKAS; P130Cas

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HeLa cells treated with TNF 2500U/ML 30', using p63 (Phospho-Ser455) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using p63 (Phospho-Ser455) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

p63, Polyclonal Antibody (Cat# AAA316271)

Full Name

p63 (Phospho-Ser455) Antibody

Gene Names

TP63; AIS; KET; LMS; NBP; RHS; p40; p51; p63; EEC3; OFC8; p73H; p73L; SHFM4; TP53L; TP73L; p53CP; TP53CP; B(p51A); B(p51B)

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HUVEC cells treated with EGF 200ng/ml 30', using HER4 (Phospho-Tyr1284) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using HER4 (Phospho-Tyr1284) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells treated with EGF 200nM 5', using HER4 (Phospho-Tyr1284) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

HER4, Polyclonal Antibody (Cat# AAA316370)

Full Name

HER4 (Phospho-Tyr1284) Antibody

Gene Names

ERBB4; HER4; ALS19; p180erbB4

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from 293 cells, using Caspase 1 (Phospho-Ser376) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using Caspase 1 (Phospho-Ser376) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

Caspase 1, Polyclonal Antibody (Cat# AAA316391)

Full Name

Caspase 1 (Phospho-Ser376) Antibody

Gene Names

CASP1; ICE; P45; IL1BC

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using BCL-2 (Phospho-Thr69) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells, using BCL-2 (Phospho-Thr69) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

BCL-2, Polyclonal Antibody (Cat# AAA316293)

Full Name

BCL-2 (Phospho-Thr69) Antibody

Gene Names

BCL2; Bcl-2; PPP1R50

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from Jurkat cells, using p38 MAPK (Phospho-Tyr322) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using p38 MAPK (Phospho-Tyr322) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

p38 MAPK, Polyclonal Antibody (Cat# AAA316301)

Full Name

p38 MAPK (Phospho-Tyr322) Antibody

Gene Names

MAPK14; RK; p38; CSBP; EXIP; Mxi2; CSBP1; CSBP2; CSPB1; PRKM14; PRKM15; SAPK2A; p38ALPHA

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HeLa cells treated with Calyculin A 50nM 30', using CDK7 (Phospho-Thr170) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using CDK7 (Phospho-Thr170) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

CDK7, Polyclonal Antibody (Cat# AAA316333)

Full Name

CDK7 (Phospho-Thr170) Antibody

Gene Names

CDK7; CAK; CAK1; HCAK; MO15; STK1; CDKN7; p39MO15

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HepG2 cells treated with Na3VO4 0.3mM 40', using FLT3 (Phospho-Tyr969) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using FLT3 (Phospho-Tyr969) Antibody. The picture on the right is blocked with the phospho peptide.)

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FLT3, Polyclonal Antibody (Cat# AAA316612)

Full Name

FLT3 (Phospho-Tyr969) Antibody

Gene Names

FLT3; FLK2; STK1; CD135; FLK-2

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from NIH/3T3 cells treated with UV 15', using PAK1 (Phospho-Ser204) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using PAK1 (Phospho-Ser204) Antibody. The picture on the right is blocked with the phospho peptide.)

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PAK1, Polyclonal Antibody (Cat# AAA316624)

Full Name

PAK1 (Phospho-Ser204) Antibody

Gene Names

PAK1; PAKalpha

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HT29 cells treated with serum 20% 15', using GRB2 (Phospho-Ser159) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using GRB2 (Phospho-Ser159) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

GRB2, Polyclonal Antibody (Cat# AAA316636)

Full Name

GRB2 (Phospho-Ser159) Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-Tau (S396) Antibody.)

WB (Western Blot) (Western blot analysis of Phospho-Tau (S396) expression in (1) SH-SY5Y cell lysate treated with AP; (2) SH-SY5Y cell lysate.)

Compare (5 max)

Tau, Monoclonal Recombinant Antibody (Cat# AAA314863)

Full Name

Tau (Phospho-Ser396) Rabbit mAb

Gene Names

MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-chromatography

Pricing

$290/0.05 mL $365/0.1 mL $705/2x0.1 mL $1,030/3x0.1 mL $1,335/4x0.1 mL $1,425/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human bladder cancer, using Phospho-SMC1 (S957) Antibody.)

WB (Western Blot) (Western blot analysis of Phospho-SMC1 (S957) expression in (1) HeLa cell lysate; (2) Hela cells lysate treated with AP.)

Compare (5 max)

SMC1, Monoclonal Recombinant Antibody (Cat# AAA314865)

Full Name

SMC1 (Phospho-Ser957) Rabbit mAb

Gene Names

SMC1A; SMC1; SMCB; CDLS2; SB1.8; SMC1L1; DXS423E; SMC1alpha

Reactivity

Human

Applications

Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Affinity-chromatography

Pricing

$290/0.05 mL $365/0.1 mL $705/2x0.1 mL $1,030/3x0.1 mL $1,335/4x0.1 mL $1,425/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human colon, using Phospho-SIRT1 (S47) Antibody.)

WB (Western Blot) (Western blot analysis of Phospho-SIRT1 (S47) expression in (1) HEK293 cell lysate; (2) HEK293 cell lysate treated with LP.)

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SIRT1, Monoclonal Recombinant Antibody (Cat# AAA314866)

Full Name

SIRT1 (Phospho-Ser47) Rabbit mAb

Gene Names

SIRT1; SIR2L1

Reactivity

Human

Applications

Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Affinity-chromatography

Pricing

$290/0.05 mL $365/0.1 mL $705/2x0.1 mL $1,030/3x0.1 mL $1,335/4x0.1 mL $1,425/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human stomach, using Phospho-YAP1 (S127) Antibody.)

WB (Western Blot) (Western blot analysis of Phospho-YAP1 (S127) expression in (1) HeLa cell lysate; (2) HeLa cell lysate treated with FBS+calyculin A.)

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YAP1, Monoclonal Recombinant Antibody (Cat# AAA314868)

Full Name

YAP1 (Phospho-Ser127) Rabbit mAb

Gene Names

YAP1; YAP; YKI; YAP2; YAP65

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-chromatography

Pricing

$290/0.05 mL $365/0.1 mL $705/2x0.1 mL $1,030/3x0.1 mL $1,335/4x0.1 mL $1,425/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescent analysis of NIH/3T3 cells treated with PDGF, using Phospho-Akt(Ser473) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human kidney, using Phospho-Akt(Ser473) Antibody.)

WB (Western Blot) (Western blot analysis on 2 NIH/3T3 cell lysate treated with PDGF using Phospho-Akt(Ser473) Antibody.)

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Akt, Monoclonal Recombinant Antibody (Cat# AAA314873)

Full Name

Akt (Phospho-Ser473) Rabbit mAb

Gene Names

AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Affinity-chromatography

Pricing

$290/0.05 mL $365/0.1 mL $705/2x0.1 mL $1,030/3x0.1 mL $1,335/4x0.1 mL $1,425/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescent analysis of HeLa cells treated with H2O2, using Phospho-Histone H2A.X (S139) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded mouse liver, using Phospho-Histone H2A.X (S139) Antibody.)

WB (Western Blot) (Western blot analysis of HistoneH2A.X phosphorylation expression in Jurkat cell lysate treated with etoposide.)

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Histone H2A.X, Monoclonal Recombinant Antibody (Cat# AAA314876)

Full Name

Histone H2A.X (Phospho-Ser139) Rabbit mAb

Gene Names

H2AFX; H2AX; H2A.X; H2A/X

Reactivity

Human, Mouse, Rat

Applications

Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Affinity-chromatography

Pricing

$290/0.05 mL $365/0.1 mL $705/2x0.1 mL $1,030/3x0.1 mL $1,335/4x0.1 mL $1,425/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescent analysis of HeLa cells treated with anisomycin, using Phospho-c-Jun (S63) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-c-Jun (S63) Antibody.)

WB (Western Blot) (Western blot analysis of c-Jun phosphorylation expression in NIH/3T3 cell lysate treated with Anisomycin.)

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c-Jun, Monoclonal Recombinant Antibody (Cat# AAA314878)

Full Name

c-Jun (Phospho-Ser63) Rabbit mAb

Gene Names

JUN; AP1; AP-1; c-Jun

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Affinity-chromatography

Pricing

$290/0.05 mL $365/0.1 mL $705/2x0.1 mL $1,030/3x0.1 mL $1,335/4x0.1 mL $1,425/5x0.1 mL

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ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence - Phospho-MCU(S92) antibody (14297) 14297 staining Phospho-MCU(S92) in the HeLa cell line by ICC/IF. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 14297 at a working dilution of 1/100. The secondary antibody was Alexa Fluor? 488 goat anti rabbit, used at a dilution of 1/500. The negative control is shown in bottom right hand panel - for the negative control, purified 14297 was used at a dilution of 1/100 followed by Alexa Fluor? 647 goat anti- rabbit antibody at a dilution of 1/500. Nuclei were counterstained with DAPI.)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded rat lung tissue stained for Phospho-MCU(S92) using 14297 at 1/100 dilution in immunohistochemical analysis.)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded mouse lung tissue stained for Phospho-MCU(S92) using 14297 at 1/100 dilution in immunohistochemical analysis.)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human lung carcinom tissue stained for Phospho-MCU(S92) using 14297 at 1/100 dilution in immunohistochemical analysis.)

WB (Western Blot) (All lanes : Phospho-MCU(S92) Rabbit mAb at 1/1k dilutionLane 1 : 3T3 whole cell lysatesLane 2 : 293T whole cell lysatesLane 3 : 293T treated with Lambda Protein Phosphatase for 30min whole cell lysatesLane 4 : MCF7 whole cell lysatesLane 5 : MCF7 treated with Lambda Protein Phosphatase for 30min whole cell lysatesLysates/proteins at 20 ug per lane.Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 40 kDaObserved band size: 32 kDaExposure time: 10 seconds)

WB (Western Blot) (All lanes : Phospho-MCU(S92) Rabbit mAb at 1/1k dilutionLane 1 : MCF7 whole cell lysatesLane 2 : 293T whole cell lysatesLane 3 : A549 whole cell lysatesLane 4 : Rat Liver lysatesLysates/proteins at 20 ug per lane.,br>Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 40 kDaObserved band size: 32 kDaExposure time: 8 seconds)

IF (Immunofluorescence) (Immunofluorescent analysis using the Antibody at 1:150 dilution.)

Application Data (All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.)

Application Data (All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.)

Application Data (All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.)

Application Data (All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.)

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MCU, Polyclonal Antibody (Cat# AAA314883)

Full Name

MCU (Phospho-Ser92) Rabbit pAb

Gene Names

MCU; C10orf42; CCDC109A

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunofluorescence, Western Blot

Purity

Affinity-chromatography

Pricing

$290/0.05 mL $365/0.1 mL $705/2x0.1 mL $1,030/3x0.1 mL $1,335/4x0.1 mL $1,425/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast, using Phospho-p53 (S392) Antibody.)

WB (Western Blot) (Western blot analysis of Phospho-p53 (Ser392) expression in HEK293 whole cell lysate.)

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p53, Monoclonal Recombinant Antibody (Cat# AAA314846)

Full Name

p53 (Phospho-Ser392) Rabbit mAb

Gene Names

TP53; P53; BCC7; LFS1; TRP53

Reactivity

Human, Mouse, Rat

Applications

Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Affinity-chromatography

Pricing

$290/0.05 mL $365/0.1 mL $705/2x0.1 mL $1,030/3x0.1 mL $1,335/4x0.1 mL $1,425/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast cancer, using Phospho-EGFR (Y1068) Antibody .)

WB (Western Blot) (Western blot analysis of EGFR phosphorylation expression in A431 cell treated with EGF.)

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EGFR, Monoclonal Recombinant Antibody (Cat# AAA314847)

Full Name

EGFR (Phospho-Tyr1068) Rabbit mAb

Gene Names

EGFR; ERBB; HER1; mENA; ERBB1; PIG61

Reactivity

Human, Mouse

Applications

Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Affinity-chromatography

Pricing

$290/0.05 mL $365/0.1 mL $705/2x0.1 mL $1,030/3x0.1 mL $1,335/4x0.1 mL $1,425/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescent analysis of A431 cells treated with EGF, using Phospho-STAT3 (S727) Antibody .)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded rat brain, using Phospho-STAT3 (S727) Antibody.)

WB (Western Blot) (Western blot analysis of Phospho-STAT3 (S727) expression in A431 cell lysate treated with EGF.)

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STAT3, Monoclonal Recombinant Antibody (Cat# AAA314849)

Full Name

STAT3 (Phospho-Ser727) Rabbit mAb

Gene Names

STAT3; APRF; HIES

Reactivity

Human, Mouse, Rat

Applications

Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Affinity-chromatography

Pricing

$290/0.05 mL $365/0.1 mL $705/2x0.1 mL $1,030/3x0.1 mL $1,335/4x0.1 mL $1,425/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded rat spleen, using Phospho-Cdk1/2 (T14) Antibody.)

WB (Western Blot) (Western blot analysis of Phospho-Cdk1/2 (T14) expression in HeLa cell lysate treated with hydroxyurea.)

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Cdk1/2, Monoclonal Recombinant Antibody (Cat# AAA314852)

Full Name

Cdk1/2 (Phospho-Thr14) Rabbit mAb

Gene Names

CDK1; CDC2; CDC28A; P34CDC2

Reactivity

Human, Mouse, Rat

Applications

Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

Affinity-chromatography

Pricing

$290/0.05 mL $365/0.1 mL $705/2x0.1 mL $1,030/3x0.1 mL $1,335/4x0.1 mL $1,425/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-c-Myc (S62) Antibody .)

WB (Western Blot) (Western blot analysis of Phospho-c-Myc (S62) expression in HeLa cell lysate.)

Compare (5 max)

c-Myc, Monoclonal Recombinant Antibody (Cat# AAA314856)

Full Name

c-Myc (Phospho-Ser62) Rabbit mAb

Gene Names

MYC; MRTL; c-Myc; bHLHe39

Reactivity

Human, Mouse, Rat

Applications

Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Affinity-chromatography

Pricing

$290/0.05 mL $365/0.1 mL $705/2x0.1 mL $1,030/3x0.1 mL $1,335/4x0.1 mL $1,425/5x0.1 mL

WB (Western Blot) (Western blot analysis of extracts from 293 cells, transfected with Tau and treated with calf intestinal phosphatase (CIP) using Tau(Phospho-Thr217) Rabbit mAb.)

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Tau, Monoclonal Antibody (Cat# AAA314726)

Full Name

Recombinant Tau (Phospho-Thr217) Rabbit mAb (G122)

Gene Names

MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Affinity purification

Pricing

$290/0.05 mL $365/0.1 mL $705/2x0.1 mL $1,030/3x0.1 mL $1,335/4x0.1 mL $1,425/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Connexin 43(Phospho-Ser367) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from 293 cells untreated or treated with PMA using Connexin 43(phospho-Ser368) Antibody.)

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Connexin 43, Polyclonal Antibody (Cat# AAA303209)

Full Name

Connexin 43 (phospho-Ser367) Antibody

Gene Names

GJA1; HSS; CMDR; CX43; EKVP; GJAL; ODDD; AVSD3; HLHS1; PPKCA

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti- phospho -SMC1 (S957) antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti- phospho -SMC1 (S957) antibody. Counter stained with hematoxylin.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti- phospho -SMC1 (S957) antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of SMC1 (phospho S957) on Hela cells lysates using anti- phospho -SMC1 (S957) antibody at 1/1, 000 dilution.)

Compare (5 max)

SMC1, Monoclonal Antibody (Cat# AAA311015)

Full Name

SMC1 (Phospho-S957) Antibody

Gene Names

SMC1A; SMC1; SMCB; CDLS2; SB1.8; SMC1L1; DXS423E; SMC1alpha

Reactivity

Human

Applications

Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

ProA affinity purified

Pricing

$365/0.05 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $1,990/5x0.1 mL

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ICC (Immunocytochemistry) (ICC staining phospho -Tau (S396) in PC12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining phospho -Tau (S396) in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti- phospho -Tau (S396) antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti- phospho -Tau (S396) antibody. Counter stained with hematoxylin.)

Compare (5 max)

Tau, Monoclonal Antibody (Cat# AAA311025)

Full Name

Tau (Phospho-S396) Antibody

Gene Names

MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17

Reactivity

Human, Mouse, Rat

Applications

Immunoprecipitation, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Western Blot

Purity

ProA affinity purified

Pricing

$365/0.05 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $1,990/5x0.1 mL

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FCM/FACS (Flow Cytometry) (Flow cytometric analysis of Hela cells with Phospho-Smad2 (S250) antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)

WB (Western Blot) (Western blot analysis of Phospho-Smad2 (S250) on Hela cells lysates using anti-Phospho-Smad2 (S250) antibody at 1/1, 000 dilution.)

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SMAD2, Monoclonal Antibody (Cat# AAA311031)

Full Name

SMAD2 (Phospho-S250) Antibody

Gene Names

SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunohistochemistry, Western Blot

Purity

ProA affinity purified

Pricing

$365/0.05 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $1,990/5x0.1 mL

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ICC (Immunocytochemistry) (ICC staining Phospho-Hsp27 (S78) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Phospho-Hsp27 (S78) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Phospho-Hsp27 (S78) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-Hsp27 (S78) antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Phospho-Hsp27 (S78) on different lysates using anti-Phospho-Hsp27 (S78) antibody at 1/1, 000 dilution. Positive control: Lane 1: Human heart Lane 2: Human skeletal muscle)

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Hsp27, Monoclonal Antibody (Cat# AAA311037)

Full Name

Hsp27 (Phospho-S78) Antibody

Gene Names

HSPB1; CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067

Reactivity

Human

Applications

Immunoprecipitation, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Western Blot

Purity

ProA affinity purified

Pricing

$365/0.05 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $1,990/5x0.1 mL

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ICC (Immunocytochemistry) (ICC staining Phospho-Rac1+Cdc42 (Ser71) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Phospho-Rac1+Cdc42 (Ser71) in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Phospho-Rac1+Cdc42 (Ser71) antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Phospho-Rac1+Cdc42 (Ser71) antibody. Counter stained with hematoxylin.)

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Rac1+Cdc42, Monoclonal Antibody (Cat# AAA311043)

Full Name

Rac1+Cdc42 (Phospho-Ser71) Antibody

Gene Names

CDC42; G25K; CDC42Hs

Reactivity

Human

Applications

Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

ProA affinity purified

Pricing

$365/0.05 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $1,990/5x0.1 mL

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ICC (Immunocytochemistry) (ICC staining Phospho-PTEN (S380) in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Phospho-PTEN (S380) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Phospho-PTEN (S380) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

WB (Western Blot) (Western blot analysis of Phospho-PTEN (S380) on different lysates using anti-Phospho-PTEN (S380) antibody at 1/1, 000 dilution. Positive control: Lane 1: Human placenta Lane 2: Human lung)

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PTEN, Monoclonal Antibody (Cat# AAA311044)

Full Name

PTEN (Phospho-S380) Antibody

Gene Names

PTEN; BZS; DEC; CWS1; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry, Western Blot

Purity

ProA affinity purified

Pricing

$365/0.05 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $1,990/5x0.1 mL

What Are Phospho Antibodies?

Protein phosphorylation is a process where a phosphate group is added to certain amino acid residues of a protein – usually serine (S), threonine (T), or tyrosine (Y) - by enzymes called kinases. This process is integral in controlling cellular signaling, cellular growth, and other biological functions.

Our catalog includes a wide range of phospho-specific antibodies that can accurately detect this important marker. They perform strongly in widely-used laboratory applications such as Western blot, flow cytometry, immunohistochemistry, and immunofluorescence microscopy. We value your trust in us and are committed to providing top-quality products and services. All of our antibodies are guaranteed to work for the applications and species indicated on our website & associated product pages.

What Are The Key Applications of Phospho Antibodies?

1. Western Blotting

One of the first steps a researcher can take in utilizing these phospho-specific antibodies, is to check if the antibody works using a technique referred to as “Western blot”. For those unfamiliar, Western Blot aids in showing whether the protein that the antibody recognizes is appearing at the correct/expected size. These phospho-specific antibodies should also be able to detect changes in the target protein’s phosphorylation (on/off state) when cells are stimulated in certain ways.

2. Staining of Fixed Cells (Immunocytochemistry)

Another routine use of these phospho-specific antibodies, is to test if the antibody is able to demonstrate similar performance when used on fixed cells (intact cells that have been preserved) as it did in the Western blot tests. It is an important aspect in many cases to confirm that the antibody works in actual intact cell samples. Ideally, the method used for cellular fixation should be the same as what is used in pathology labs (like using 10% formalin). To check if the antibody works well in tissue sections (FFPE), researchers will often test it on fixed cells that are processed similar to tissue samples.

3. Specificity Tests Using Peptides

In order to make sure that the antibody is only binding to the right target:

• Laboratory technicians will mix the antibody with phospho-peptides (short segments of the protein containing the phosphate group modification).


• If the antibody signal disappears, it is confirmation that it is binding to the correct phosphorylated location.


• A more robust test is to use both the phosphorylated and non-phosphorylated (dephosphorylated) versions of the protein. The antibody should react only with the phosphorylated one.


• Another method sometimes utilized is to treat the sample with an enzyme, such as alkaline phosphatase, that specifically removes phosphate groups. If the antibody signal disappears after this, it also confirms specificity.

4. Genetic Confirmation

As a final step, scientists can genetically manipulate the nucleotide sequence and alter the target protein by removing the exact site where phosphorylation happens. If the antibody no longer appears to detect the modified protein, it is strong evidence supporting the antibody being specific for that phosphorylated site.

Why Buy Phospho Antibodies Through Us?

• The production laboratory adheres to strict and consistent protocols prior to releasing any of these phospho-specific antibodies:


• Standard methods and proper controls in all tests to ensure high quality.


• These antibodies are tested and validated in different cell types and species.


• High quality control criterion to ensure each batch is consistent, so you will obtain reliable results every time.

FAQ

1. What Are Phospho-Specific Antibodies?

Phospho-specific antibodies are made to detect proteins only when they have a phosphate group linked to a specific amino acid residue. This empowers scientists understand if a protein is "turned on" or active, based on its phosphorylation state.

2. How to Detect Phosphorylated Proteins in a Western Blot?

To find out if a protein is phosphorylated using Western blot:

• Use a phospho-specific antibody that binds only to the phosphorylated form of the protein.


• You can also use a “regular” antibody for the same amino acid sequence of the protein that the phospho-specific antibody is binding to (but in this case, this antibody will not bind if there is a phosphate group present) in order to compare how much of it is phosphorylated versus how much is non-phosphorylated (or “total” protein, if the “normal” antibody’s epitopes are non-phospho-site-specific).

3. How to Choose the Best Antibody?

Here are some simple tips to help you pick the right antibody:

• Know your target


• Match your sample characteristics


• Confirm the intended use is appropriate


• Check “host” and “type”


• Check the “quality” of the presented data/images


• Appraise whether the available validation meets your needs