Phospho Antibodies

Phospho-specific antibodies’ typical purpose is to enable researchers to detect changes in proteins. They will exclusively bind to the amino acid sequence on a protein that has been phosphorylated (which is both a physical & chemical change) and do not bind to the same amino acid sequence on said protein if it lacks said phosphorylation. This aids in being able to clearly see and understand the data produced from this particular protein modification.

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WB (Western Blot) (Western blot analysis of lysates from Jurkat cells treated with starved 24h, using PKC delta (Phospho-Tyr52) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using PKC delta (Phospho-Tyr52) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells, using PKC delta (Phospho-Tyr52) Antibody. The picture on the right is blocked with the phospho peptide.)

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PKC delta, Polyclonal Antibody (Cat# AAA316302)

Full Name

PKC delta (Phospho-Tyr52) Antibody

Gene Names

PRKCD; MAY1; PKCD; ALPS3; CVID9; nPKC-delta

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from Jurkat cells treated with Etoposide 25uM 60', using Caspase 3 (Phospho-Ser150) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human lymph node, using Caspase 3 (Phospho-Ser150) Antibody. The picture on the right is blocked with the phospho peptide.)

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Caspase 3, Polyclonal Antibody (Cat# AAA316324)

Full Name

Caspase 3 (Phospho-Ser150) Antibody

Gene Names

CASP3; CPP32; SCA-1; CPP32B

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

WB (Western Blot) (Western blot analysis of extracts from Hela cells, untreated (-) or treated, 1:5000. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).)

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Histone H4, ELISA Kit (Cat# AAA319063)

Full Name

Histone H4 (Phospho-Thr80) Colorimetric Cell-Based ELISA Kit

Gene Names

HIST2H4B; H4/o

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of extracts from Hela cells, untreated (-) or treated, 1:2000. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).)

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Histone H3, ELISA Kit (Cat# AAA319067)

Full Name

Histone H3 (Phospho-Ser10) Colorimetric Cell-Based ELISA Kit

Gene Names

HIST1H3A; H3/A; H3FA

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4 degree C over night)

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Axl, ELISA Kit (Cat# AAA319076)

Full Name

Axl (Phospho-Tyr702) Colorimetric Cell-Based ELISA Kit

Gene Names

AURKA; AIK; ARK1; AURA; BTAK; STK6; STK7; STK15; AURORA2; PPP1R47

Reactivity

Human, Rat, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4 degree C over night)

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Prdx1, ELISA Kit (Cat# AAA319077)

Full Name

Prdx1 (Phospho-Tyr194) Colorimetric Cell-Based ELISA Kit

Gene Names

PRDX1; PAG; PAGA; PAGB; PRX1; PRXI; MSP23; NKEFA; TDPX2; NKEF-A

Reactivity

Human, Rat, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

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IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4 degree overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4 degree overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast cancer, using VAV1 (Phospho-Tyr174) Antibody. The picture on the right is blocked with the VAV1 (Phospho-Tyr174) peptide.)

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Vav, Polyclonal Antibody (Cat# AAA318645)

Full Name

Vav (Phospho-Tyr174) Rabbit Polyclonal Antibody

Gene Names

VAV1; VAV

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)

WB (Western Blot) (Western Blot analysis of 1 HEK-293 cell, 2, LPS 100ng/mL 30min treated ,using primary antibody at 1:1000 dilution. Secondary antibody)

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CEP55, Polyclonal Antibody (Cat# AAA318654)

Full Name

CEP55 (Phospho-Ser425) Rabbit Polyclonal Antibody

Gene Names

CEP55; CT111; URCC6; C10orf3

Reactivity

Human, Rat, Mouse

Applications

Western Blot, Immunofluorescence, Immunohistochemistry

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from K562 cells treated with EGF 200ng/ml 5', using p70 S6 Kinase beta (Phospho-Ser423) Antibody.)

ELISA (Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using p70 S6 Kinase beta (Phospho-Ser423) Antibody)

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p70 S6 Kinase beta, Polyclonal Antibody (Cat# AAA318369)

Full Name

p70 S6 Kinase beta (Phospho-Ser423) Antibody

Gene Names

RPS6KB2; KLS; SRK; S6K2; STK14B; p70S6Kb; P70-beta; S6K-beta2; P70-beta-1; P70-beta-2; p70(S6K)-beta

Reactivity

Human, Mouse

Applications

Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$345/0.05 mL $450/0.1 mL $880/2x0.1 mL $1,285/3x0.1 mL $1,660/4x0.1 mL $1,775/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from LOVO cells treated with starved 24h, using PAK1 (Phospho-Ser199) Antibody.)

ELISA (Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PAK1 (Phospho-Ser199) Antibody)

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PAK1, Polyclonal Antibody (Cat# AAA318370)

Full Name

PAK1 (Phospho-Ser199) Antibody

Gene Names

PAK1; PAKalpha

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$345/0.05 mL $450/0.1 mL $880/2x0.1 mL $1,285/3x0.1 mL $1,660/4x0.1 mL $1,775/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from Jurkat cells treated with PMA, using PLK1 (Phospho-Ser137) Antibody.)

ELISA (Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PLK1 (Phospho-Ser137) Antibody)

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PLK1, Polyclonal Antibody (Cat# AAA318377)

Full Name

PLK1 (Phospho-Ser137) Antibody

Gene Names

PLK1; PLK; STPK13

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$345/0.05 mL $450/0.1 mL $880/2x0.1 mL $1,285/3x0.1 mL $1,660/4x0.1 mL $1,775/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HepG2 cells treated with nocodazole 1ug/ml 16h, using Retinoblastoma (Phospho-Thr826) Antibody.)

ELISA (Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Retinoblastoma (Phospho-Thr826) Antibody)

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Retinoblastoma, Polyclonal Antibody (Cat# AAA318379)

Full Name

Retinoblastoma (Phospho-Thr826) Antibody

Gene Names

RB1; RB; pRb; OSRC; pp110; p105-Rb; PPP1R130

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$345/0.05 mL $450/0.1 mL $880/2x0.1 mL $1,285/3x0.1 mL $1,660/4x0.1 mL $1,775/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HepG2 cells treated with TNF 200NG/ML 30', using SHIP1 (Phospho-Tyr1021) Antibody.)

ELISA (Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using SHIP1 (Phospho-Tyr1021) Antibody)

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SHIP1, Polyclonal Antibody (Cat# AAA318385)

Full Name

SHIP1 (Phospho-Tyr1021) Antibody

Gene Names

INPP5D; SHIP; SHIP1; SHIP-1; hp51CN; SIP-145; p150Ship

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$345/0.05 mL $450/0.1 mL $880/2x0.1 mL $1,285/3x0.1 mL $1,660/4x0.1 mL $1,775/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from Jurkat cells, using Smad3 (Phospho-Ser208) Antibody.)

ELISA (Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Smad3 (Phospho-Ser208) Antibody)

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Smad3, Polyclonal Antibody (Cat# AAA318387)

Full Name

Smad3 (Phospho-Ser208) Antibody

Gene Names

SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$345/0.05 mL $450/0.1 mL $880/2x0.1 mL $1,285/3x0.1 mL $1,660/4x0.1 mL $1,775/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from mouse brain, using Synuclein-alpha (Phospho-Tyr136) Antibody.)

ELISA (Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Synuclein-alpha (Phospho-Tyr136) Antibody)

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Synuclein-alpha, Polyclonal Antibody (Cat# AAA318393)

Full Name

Synuclein-alpha (Phospho-Tyr136) Antibody

Gene Names

SNCA; PD1; NACP; PARK1; PARK4

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$345/0.05 mL $450/0.1 mL $880/2x0.1 mL $1,285/3x0.1 mL $1,660/4x0.1 mL $1,775/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HepG2 cells treated with TNF 20ng/ml 5', using TAK1 (Phospho-Thr184) Antibody.)

ELISA (Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using TAK1 (Phospho-Thr184) Antibody)

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TAK1, Polyclonal Antibody (Cat# AAA318394)

Full Name

TAK1 (Phospho-Thr184) Antibody

Gene Names

MAP3K7; CSCF; FMD2; TAK1; MEKK7; TGF1a

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$345/0.05 mL $450/0.1 mL $880/2x0.1 mL $1,285/3x0.1 mL $1,660/4x0.1 mL $1,775/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from Jurkat cells treated with paclitaxel 1uM 24h, using TOP2A (Phospho-Ser1106) Antibody.)

ELISA (Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using TOP2A (Phospho-Ser1106) Antibody)

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TOP2A, Polyclonal Antibody (Cat# AAA318399)

Full Name

TOP2A (Phospho-Ser1106) Antibody

Gene Names

TOP2A; TOP2; TP2A

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$345/0.05 mL $450/0.1 mL $880/2x0.1 mL $1,285/3x0.1 mL $1,660/4x0.1 mL $1,775/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from mouse brain, using Trk A (Phospho-Tyr701) Antibody.)

ELISA (Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Trk A (Phospho-Tyr701) Antibody)

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Trk A, Polyclonal Antibody (Cat# AAA318403)

Full Name

Trk A (Phospho-Tyr701) Antibody

Gene Names

NTRK1; MTC; TRK; TRK1; TRKA; Trk-A; p140-TrkA

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$345/0.05 mL $450/0.1 mL $880/2x0.1 mL $1,285/3x0.1 mL $1,660/4x0.1 mL $1,775/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from 293 cells treated with etoposide 25uM 60', using WEE1 (Phospho-Ser642) Antibody.)

ELISA (Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using WEE1 (Phospho-Ser642) Antibody)

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WEE1, Polyclonal Antibody (Cat# AAA318407)

Full Name

WEE1 (Phospho-Ser642) Antibody

Gene Names

WEE1; WEE1A; WEE1hu

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$345/0.05 mL $450/0.1 mL $880/2x0.1 mL $1,285/3x0.1 mL $1,660/4x0.1 mL $1,775/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HepG2 cells treated with PMA 125ng/ml 15', using YB1 (Phospho-Ser102) Antibody.)

ELISA (Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using YB1 (Phospho-Ser102) Antibody)

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YB1, Polyclonal Antibody (Cat# AAA318409)

Full Name

YB1 (Phospho-Ser102) Antibody

Gene Names

YBX1; YB1; BP-8; CSDB; DBPB; YB-1; CBF-A; CSDA2; EFI-A; NSEP1; NSEP-1; MDR-NF1

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$345/0.05 mL $450/0.1 mL $880/2x0.1 mL $1,285/3x0.1 mL $1,660/4x0.1 mL $1,775/5x0.1 mL

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IHC (Immunohiostchemistry) (AAA321408 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of extracts from L-540 cells, using Phospho-TNK1 (Tyr277) Antibody. Lane1 was treated with phospho-blocking peptide, Lane 2 was treated with non-phospho-blocking peptide.)

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TNK1, Polyclonal Antibody (Cat# AAA321408)

Full Name

Phospho-TNK1 (Tyr277) Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Peptide affinity purification

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of Phospho-EGFR (Tyr1110) expression in various lysates)

WB (Western Blot) (Western blot analysis of EGFR phosphorylation expression in EGF treated A431 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA321451 staining SK-OV3 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

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EGFR, Polyclonal Antibody (Cat# AAA321451)

Full Name

Phospho-EGFR (Tyr1110) Antibody

Gene Names

EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of Fos phosphorylation expression in forskolin treated K562 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA321455 staining K562 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

IHC (Immunohiostchemistry) (AAA321455 at 1/100 staining human liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of Phospho-Fos (Ser362) expression in various lysates)

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FOS, Polyclonal Antibody (Cat# AAA321455)

Full Name

Phospho-FOS (Ser362) Antibody

Gene Names

FOS; p55; AP-1; C-FOS

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of Estrogen Receptor alpha phosphorylation expression in EGF treated MCF7 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA321456 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

IHC (Immunohistochemistry) (AAA321456 at 1/100 staining human breast carcinoma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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Estrogen Receptor alpha, Polyclonal Antibody (Cat# AAA321456)

Full Name

Phospho-Estrogen Receptor alpha (Ser167) Antibody

Gene Names

ESR1; ER; ESR; Era; ESRA; ESTRR; NR3A1

Reactivity

Human

Predicted to cross-react with canine, goat, porcine and sheep based on sequence homology.

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohiostchemistry) (AAA321310 at 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of extracts of mouse brain tissue, using Phospho-BMAL1 (Ser42) Antibody.)

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BMAL1, Polyclonal Antibody (Cat# AAA321310)

Full Name

Phospho-BMAL1 (Ser42) Antibody

Gene Names

ARNTL; TIC; JAP3; MOP3; BMAL1; PASD3; BMAL1c; bHLHe5

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of Phospho-Met (Tyr1349) expression in various lysates)

WB (Western Blot) (Western blot analysis of Met phosphorylation expression in HepG2 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA321502 staining NIH-3T3 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

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Met, Polyclonal Antibody (Cat# AAA321502)

Full Name

Phospho-Met (Tyr1349) Antibody

Gene Names

MET; HGFR; AUTS9; RCCP2; c-Met; DFNB97

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohistochemistry) (AAA321503 at 1/100 staining Human kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemisry) (AAA321503 at 1/100 staining human breast carcinoma tissues sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 ho)

WB (Western Blot) (Western blot analysis of Vimentin phosphorylation expression in Nocodazole treated A549 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA321503 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

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Vimentin, Polyclonal Antibody (Cat# AAA321503)

Full Name

Phospho-Vimentin (Ser56) Antibody

Reactivity

Human

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of Phospho-Stathmin 1 (Ser15) expression in various lysates)

IHC (Immunohistochemisry) (AAA321541 at 1/200 staining human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IF (Immunofluorescence) (AAA321541 staining COS7 cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)

WB (Western Blot) (Western blot analysis of Stathmin 1 phosphorylation expression in 293 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

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Stathmin 1, Polyclonal Antibody (Cat# AAA321541)

Full Name

Phospho-Stathmin 1 (Ser15) Antibody

Gene Names

STMN1; Lag; SMN; OP18; PP17; PP19; PR22; LAP18; C1orf215

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohistochemistry) (AAA321556 at 1/100 staining human lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of Elk1 phosphorylation expression in UV treated Jurkat whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA321556 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of Phospho-Elk1 (Ser389) expression in various lysates)

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Elk1, Polyclonal Antibody (Cat# AAA321556)

Full Name

Phospho-Elk1 (Ser389) Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunoprecipitation, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of Phospho-EFNB1/2 (Tyr330) expression in various lysates)

WB (Western Blot) (Western blot analysis of EFNB1/2 phosphorylation expression in TNF-a treated 293 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

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EFNB1/2, Polyclonal Antibody (Cat# AAA321649)

Full Name

Phospho-EFNB1/2 (Tyr330) Antibody

Gene Names

EFNB2; HTKL; EPLG5; Htk-L; LERK5

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IF (Immunofluorescence) (AAA321684 staining HuvEc cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

WB (Western Blot) (Western blot analysis of Phospho-Caveolin-1 (Tyr14) expression in various lysates)

WB (Western Blot) (Western blot analysis of Caveolin-1 phosphorylation expression in H2O2 treated NIH-3T3 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

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Caveolin-1, Polyclonal Antibody (Cat# AAA321684)

Full Name

Phospho-Caveolin-1 (Tyr14) Antibody

Gene Names

CAV1; CGL3; PPH3; BSCL3; LCCNS; VIP21; MSTP085

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of extracts of Ramos cells, using Phospho-PLCgamma2 (Tyr1217) Antibody.)

IHC (Immunohistochemistry) (AAA321368 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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PLCgamma2, Polyclonal Antibody (Cat# AAA321368)

Full Name

Phospho-PLCgamma2 (Tyr1217) Antibody

Gene Names

PLCG2; FCAS3; APLAID; PLC-IV; PLC-gamma-2

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Detection of human Rad17, Phospho (S645) by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells treated with 100 uM etoposide for 2 hours (+) or mock treated (-) cells. Antibodies: Affinity purified rabbit anti-Rad17, Phospho (S645) antibody AAA210790 (lot AAA210790-2) used for WB at 0.4 ug/ml. To examine total Rad17, the membrane was reblotted with goat anti-Rad17 antibody BL239 (lower panel). Detection: Chemiluminescence with exposure times of 30 seconds (upper and lower panels).)

IHC (Immunohiostchemistry) (Detection of mouse Phospho Rad17 (S645) by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-Phospho Rad17 (S645) (Cat. No. AAA210790 Lot2) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)

IHC (Immunohistochemistry) (Detection of human Phospho Rad17 (S645) by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-Phospho Rad17 (S645) (Cat. No. AAA210790 Lot2) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)

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Rad17, Polyclonal Antibody (Cat# AAA210790)

Full Name

Rabbit anti-Phospho Rad17 (S645) Antibody, Affinity Purified

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purified

Pricing

$255/0.01 mg $635/0.1 mg $940/5x0.01 mg $1,230/2x0.1 mg $1,800/3x0.1 mg $2,335/4x0.1 mg $2,630/5x0.1 mg

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed HeLa cells using Phospho-RELA-S536 antibody.)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human breast carcinoma using Phospho-RELA-S536 antibody.)

WB (Western Blot) (Western blot analysis of extracts from HepG2 cells using Phospho-RELA-S536 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)

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RELA-S536, Antibody (Cat# AAA37345)

Full Name

Phospho-RELA-S536 Polyclonal Antibody

Gene Names

RELA; p65; NFKB3

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity Purification

Pricing

$250/0.02 mL $475/0.1 mL $925/2x0.1 mL $1,355/3x0.1 mL $1,755/4x0.1 mL $1,935/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed HeLa cells using Phospho-NFKB1-S337 antibody.)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human breast carcinoma using Phospho-NFKB1-S337 antibody.)

WB (Western Blot) (Western blot analysis of extracts of HeLa cells, using Phospho-NFKB1-S337 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)

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NFKB1-S337, Antibody (Cat# AAA37350)

Full Name

Phospho-NFKB1-S337 Polyclonal Antibody

Gene Names

NFKB1; p50; KBF1; p105; EBP-1; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity Purification

Pricing

$250/0.02 mL $475/0.1 mL $925/2x0.1 mL $1,355/3x0.1 mL $1,755/4x0.1 mL $1,935/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human breast carcinoma using Phospho-STAT4-Y693 antibody.)

WB (Western Blot) (Western blot analysis of extracts from HepG2 cells using Phospho-STAT4-Y693 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)

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STAT4-Y693, Antibody (Cat# AAA37356)

Full Name

Phospho-STAT4-Y693 Polyclonal Antibody

Gene Names

STAT4; SLEB11

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity Purification

Pricing

$250/0.02 mL $475/0.1 mL $925/2x0.1 mL $1,355/3x0.1 mL $1,755/4x0.1 mL $1,935/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed HeLa cells showing centrosome and nuclear staining using Phospho-MAPK3-Y204 antibody.)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human breast carcinoma using Phospho-MAPK3-Y204 antibody.)

WB (Western Blot) (Western blot analysis of extracts of Jurkat cells, using Phospho-MAPK3-Y204 antibody at 1:2000 dilution. Jurkat cells were treated by PMA/TPA (200nM) for 10 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 30s.)

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MAPK3-Y204, Antibody (Cat# AAA37373)

Full Name

Phospho-MAPK3-Y204 Polyclonal Antibody

Gene Names

MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity Purification

Pricing

$250/0.02 mL $475/0.1 mL $925/2x0.1 mL $1,355/3x0.1 mL $1,755/4x0.1 mL $1,935/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human breast carcinoma using Phospho-PTPN11-Y542 antibody.)

WB (Western Blot) (Western blot analysis of extracts from 293 cells using Phospho-PTPN11-Y542 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)

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PTPN11-Y542, Antibody (Cat# AAA37392)

Full Name

Phospho-PTPN11-Y542 Polyclonal Antibody

Gene Names

PTPN11; CFC; NS1; SHP2; BPTP3; PTP2C; PTP-1D; SH-PTP2; SH-PTP3

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity Purification

Pricing

$250/0.02 mL $475/0.1 mL $925/2x0.1 mL $1,355/3x0.1 mL $1,755/4x0.1 mL $1,935/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed MCF-7 cells using Phospho-KDR-Y1175 antibody.)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human breast carcinoma using Phospho-KDR-Y1175 antibody.)

WB (Western Blot) (Western blot analysis of extracts of HUVE cells, using Phospho-KDR-Y1175 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)

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KDR-Y1175, Antibody (Cat# AAA37408)

Full Name

Phospho-KDR-Y1175 Polyclonal Antibody

Gene Names

KDR; FLK1; CD309; VEGFR; VEGFR2

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity Purification

Pricing

$250/0.02 mL $475/0.1 mL $925/2x0.1 mL $1,355/3x0.1 mL $1,755/4x0.1 mL $1,935/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of A431 cells stimulated with pervanadate (1mM) for 30 minutes (lanes 1, 3, & 5) then treated with alkaline phosphatase (lanes 2, 4, & 6). The blot was probed with anti-γ-Catanine , anti-β-Catenine (Tyr-489) conserved site , or anti-β-Catenine .)

WB (Western Blot) (Westem blot analysis of γ-Catenin immunoprecipitated from A431 cells treated with pervanadate using anti-γ-Catenin (C-terminal). The immunoprecipitates were untreated (lanes 1, 3, & 7) or treated with alkaline phosphatase (lanes 2, 4, & 8). Blots of the immunoprecipitates were probed with anti-γ-Catenin (a.a. 545-555), anti-γ-Catenin (Tyr-550) or anti-γ-Catenin (C-terminal). In addition, the anti-γ-Catenin (Tyr-550) was used in the presence of phospho-γ-Catenin (Tyr-550) peptide (lane 5) or phospho-γ-Catenin (Tyr-644) peptide (lane6).)

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gamma-Catenin Phospho-Regulation, Antibody Sampler Kit (Cat# AAA71572)

Full Name

gamma-Catenin Phospho-Regulation

Applications

Immunoprecipitation, Immunocytochemistry, Western Blot

Pricing

$650/1 Kit $1,260/2x1 Kit $1,845/3x1 Kit $2,390/4x1 Kit $2,950/5x1 Kit

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WB (Western Blot) (Western blot image of C2C12 untreated (lanes 1 & 3) or treated with calyculin A (100nM) for 30 min. (lanes 2 & 4). The blot was probed with anti-eEF2K (N-terminus) (lanes 1 & 2) and anti-eEF2K (Ser-500) (lanes 3 & 4).)

Application Data (Recombinant human eEF2K untreated (lanes 1 & 4), auto-phosphorylated in the presence of Ca2+ and Calmodulin (lanes 2 & 5), and dephosphorylated with lambda phosphatase (lanes 3 & 6). The blots were probed with rabbit polyclonal anti-eEF2K (N-terminus) (lanes 1-3) or anti-eEF2K (Ser-500) (lanes 4-6). (Images provided by the laboratory of Dr. Kevin Dalby in the Dept. of Pharmacy at the University of Texas at Austin.))

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eEF2K, Polyclonal Antibody (Cat# AAA71634)

Full Name

eEF2K (Ser-500), phospho-specific Antibody

Gene Names

EEF2K; eEF-2K; HSU93850

Reactivity

Human, Rat, Mouse

Applications

Western Blot

Pricing

$505/0.1 mL $985/2x0.1 mL $1,440/3x0.1 mL $1,875/4x0.1 mL $2,100/5x0.1 mL

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IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse testis using Phospho-c-Myc-T58 Rabbit pAb (AAA36754) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.)

IHC (Immunohistochemisry) (Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Phospho-c-Myc-T58 Rabbit pAb (AAA36754) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded Rat testis using Phospho-c-Myc-T58 Rabbit pAb (AAA36754) at dilution of 1:100 (40xlens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.)

WB (Western Blot) (Western blot analysis of lysates from 293T cells, using Phospho-c-Myc-T58 Rabbit pAb (AAA36754) at 1:500 dilution. 293T cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 180s.)

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MYC-T58, Antibody (Cat# AAA36754)

Full Name

Phospho-MYC-T58 Polyclonal Antibody

Gene Names

MYC; MRTL; MYCC; c-Myc; bHLHe39

Applications

Immunofluorescence, Western Blot

Purity

Affinity Purification

Pricing

$250/0.02 mL $475/0.1 mL $925/2x0.1 mL $1,355/3x0.1 mL $1,755/4x0.1 mL $1,935/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of Cyclin B1 (pS126) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot) (Western blot analysis of Cyclin B1 (pS126) expression in HT29 nocodazole-treated (A), Jurkat (B), HeLa (C), Raw264.7 (D) whole cell lysates.)

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Cyclin B1 (pS126), Polyclonal Antibody (Cat# AAA104411)

Full Name

Anti-Cyclin B1 (pS126) Phospho Antibody

Gene Names

CCNB1; CCNB

Reactivity

Human, Mouse

Applications

Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

$240/0.03 mL $275/0.05 mL $345/0.1 mL $475/0.2 mL $925/2x0.2 mL $1,355/3x0.2 mL $1,755/4x0.2 mL $1,920/5x0.2 mL

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ELISA (Enzyme-Linked Immunosorbent Assay (ELISA) for immunogen phosphor-peptide (left) and non-phospho peptide (right), using Anti-SYK (Phospho-Tyr348) Antibody.)

Application Data (HeLa cells were grown to 90% confluency and were stimulated with EGF 200ng/mL and incubated for 30 mins. Cells were immediately lysed thereafter and measured for Total Protein Concentration and O.D. 450 nm of Src P-Tyr418 versus untreated HeLa lysates.)

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SYK, ELISA Kit (Cat# AAA318613)

Full Name

SYK (Phospho-Tyr348) Phospho Sandwich ELISA Kit

Gene Names

SYK; p72-Syk

Reactivity

Human, Mouse, Rat

Pricing

$540/1 Kit $1,040/2x1 Kit $1,515/3x1 Kit $1,965/4x1 Kit $2,445/5x1 Kit

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<< Previous Next >>

ELISA (Enzyme-Linked Immunosorbent Assay (ELISA) for immunogen phosphor-peptide (left) and non-phospho peptide (right), using Anti-Myc (Phospho-Thr58) Antibody.)

Application Data (HeLa cells were grown to 90% confluency and were stimulated with EGF 200 ng/mL for 30 minutes. Cells were immediately lysed thereafter and measured for Total Protein Concentration and O.D. 450 nm of Myc P-Thr58 versus untreated HeLa lysates.)

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Myc, ELISA Kit (Cat# AAA318625)

Full Name

Myc (Phospho-Thr58) Phospho Sandwich ELISA Kit

Gene Names

MYC; MRTL; MYCC; c-Myc; bHLHe39

Reactivity

Human, Mouse, Rat

Pricing

$540/1 Kit $1,040/2x1 Kit $1,515/3x1 Kit $1,965/4x1 Kit $2,445/5x1 Kit

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<< Previous Next >>

WB (Western Blot) (Western Blot analysis of various cells using Phospho-Neu (Y1221/Y1222) Polyclonal Antibody)

ELISA (Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using HER2 (Phospho-Tyr1221+Tyr1222) Antibody)

IF (Immunofluorescence) (Immunofluorescence analysis of HuvEc cell, using HER2 (Phospho-Tyr1221+Tyr1222) Antibody. The lane on the right is blocked with the HER2 (Phospho-Tyr1221+Tyr1222) peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast cancer, using HER2 (Phospho-Tyr1221+Tyr1222) Antibody. The picture on the right is blocked with the HER2 (Phospho-Tyr1221+Tyr1222) peptide.)

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Neu, Polyclonal Antibody (Cat# AAA318639)

Full Name

Neu (Phospho-Tyr1221/Y1222) Rabbit Polyclonal Antibody

Gene Names

ERBB2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu

Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from HepG2 cells treated with TNF 20ng/ml 5', using p47 phox (Phospho-Ser345) Antibody.)

ELISA (Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using p47 phox (Phospho-Ser345) Antibody)

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p47 phox, Polyclonal Antibody (Cat# AAA318368)

Full Name

p47 phox (Phospho-Ser345) Antibody

Gene Names

NCF1; NCF1A; NOXO2; p47phox; SH3PXD1A

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$345/0.05 mL $450/0.1 mL $880/2x0.1 mL $1,285/3x0.1 mL $1,660/4x0.1 mL $1,775/5x0.1 mL

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<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from COS7 cells treated with PMA 125ng/ml 30', using PKA-R2 beta (Phospho-Ser113) Antibody.)

ELISA (Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PKA-R2beta (Phospho-Ser113) Antibody)

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PKA-R2beta, Polyclonal Antibody (Cat# AAA318374)

Full Name

PKA-R2beta (Phospho-Ser113) Antibody

Gene Names

PRKAR2B; PRKAR2; RII-BETA

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$345/0.05 mL $450/0.1 mL $880/2x0.1 mL $1,285/3x0.1 mL $1,660/4x0.1 mL $1,775/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from Jurkat cells treated with EGF 200ng/ml 15', using PKC theta (Phospho-Ser695) Antibody.)

ELISA (Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PKC theta (Phospho-Ser695) Antibody)

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PKC theta, Polyclonal Antibody (Cat# AAA318375)

Full Name

PKC theta (Phospho-Ser695) Antibody

Gene Names

PRKCQ; PRKCT; nPKC-theta

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$345/0.05 mL $450/0.1 mL $880/2x0.1 mL $1,285/3x0.1 mL $1,660/4x0.1 mL $1,775/5x0.1 mL

What Are Phospho Antibodies?

Protein phosphorylation is a process where a phosphate group is added to certain amino acid residues of a protein – usually serine (S), threonine (T), or tyrosine (Y) - by enzymes called kinases. This process is integral in controlling cellular signaling, cellular growth, and other biological functions.

Our catalog includes a wide range of phospho-specific antibodies that can accurately detect this important marker. They perform strongly in widely-used laboratory applications such as Western blot, flow cytometry, immunohistochemistry, and immunofluorescence microscopy. We value your trust in us and are committed to providing top-quality products and services. All of our antibodies are guaranteed to work for the applications and species indicated on our website & associated product pages.

What Are The Key Applications of Phospho Antibodies?

1. Western Blotting

One of the first steps a researcher can take in utilizing these phospho-specific antibodies, is to check if the antibody works using a technique referred to as “Western blot”. For those unfamiliar, Western Blot aids in showing whether the protein that the antibody recognizes is appearing at the correct/expected size. These phospho-specific antibodies should also be able to detect changes in the target protein’s phosphorylation (on/off state) when cells are stimulated in certain ways.

2. Staining of Fixed Cells (Immunocytochemistry)

Another routine use of these phospho-specific antibodies, is to test if the antibody is able to demonstrate similar performance when used on fixed cells (intact cells that have been preserved) as it did in the Western blot tests. It is an important aspect in many cases to confirm that the antibody works in actual intact cell samples. Ideally, the method used for cellular fixation should be the same as what is used in pathology labs (like using 10% formalin). To check if the antibody works well in tissue sections (FFPE), researchers will often test it on fixed cells that are processed similar to tissue samples.

3. Specificity Tests Using Peptides

In order to make sure that the antibody is only binding to the right target:

• Laboratory technicians will mix the antibody with phospho-peptides (short segments of the protein containing the phosphate group modification).


• If the antibody signal disappears, it is confirmation that it is binding to the correct phosphorylated location.


• A more robust test is to use both the phosphorylated and non-phosphorylated (dephosphorylated) versions of the protein. The antibody should react only with the phosphorylated one.


• Another method sometimes utilized is to treat the sample with an enzyme, such as alkaline phosphatase, that specifically removes phosphate groups. If the antibody signal disappears after this, it also confirms specificity.

4. Genetic Confirmation

As a final step, scientists can genetically manipulate the nucleotide sequence and alter the target protein by removing the exact site where phosphorylation happens. If the antibody no longer appears to detect the modified protein, it is strong evidence supporting the antibody being specific for that phosphorylated site.

Why Buy Phospho Antibodies Through Us?

• The production laboratory adheres to strict and consistent protocols prior to releasing any of these phospho-specific antibodies:


• Standard methods and proper controls in all tests to ensure high quality.


• These antibodies are tested and validated in different cell types and species.


• High quality control criterion to ensure each batch is consistent, so you will obtain reliable results every time.

FAQ

1. What Are Phospho-Specific Antibodies?

Phospho-specific antibodies are made to detect proteins only when they have a phosphate group linked to a specific amino acid residue. This empowers scientists understand if a protein is "turned on" or active, based on its phosphorylation state.

2. How to Detect Phosphorylated Proteins in a Western Blot?

To find out if a protein is phosphorylated using Western blot:

• Use a phospho-specific antibody that binds only to the phosphorylated form of the protein.


• You can also use a “regular” antibody for the same amino acid sequence of the protein that the phospho-specific antibody is binding to (but in this case, this antibody will not bind if there is a phosphate group present) in order to compare how much of it is phosphorylated versus how much is non-phosphorylated (or “total” protein, if the “normal” antibody’s epitopes are non-phospho-site-specific).

3. How to Choose the Best Antibody?

Here are some simple tips to help you pick the right antibody:

• Know your target


• Match your sample characteristics


• Confirm the intended use is appropriate


• Check “host” and “type”


• Check the “quality” of the presented data/images


• Appraise whether the available validation meets your needs