Phospho Antibodies

Phospho-specific antibodies’ typical purpose is to enable researchers to detect changes in proteins. They will exclusively bind to the amino acid sequence on a protein that has been phosphorylated (which is both a physical & chemical change) and do not bind to the same amino acid sequence on said protein if it lacks said phosphorylation. This aids in being able to clearly see and understand the data produced from this particular protein modification.

WB (Western Blot) (Western blot analysis of extracts from Hela cells, untreated (-) or treated, 1:2000. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).)

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Histone H3, ELISA Kit (Cat# AAA319074)

Full Name

Histone H3 (Phospho-Thr6) Colorimetric Cell-Based ELISA Kit

Gene Names

HIST1H3A; H3/A; H3FA

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of extracts from Hela cells, untreated (-) or treated, 1:2000. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).)

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Histone H3, ELISA Kit (Cat# AAA319075)

Full Name

Histone H3 (Phospho-Tyr41) Colorimetric Cell-Based ELISA Kit

Gene Names

HIST1H3A; H3/A; H3FA

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

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WB (Western Blot) (Western Blot analysis of Hela treated or untreated by LPS lysis, using primary antibody at 1:1000 dilution. Secondary antibody)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).)

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PRK1, Polyclonal Antibody (Cat# AAA319478)

Full Name

PRK1 (Phospho-Thr774)/PRK2 (Phospho-Thr816) Antibody

Gene Names

PKN1; DBK; PKN; PAK1; PRK1; PAK-1; PRKCL1; PKN-ALPHA

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Pricing

$300/0.05 mL $420/0.1 mL $660/0.2 mL $1,285/2x0.2 mL $1,875/3x0.2 mL $2,430/4x0.2 mL $2,735/5x0.2 mL

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WB (Western Blot) (Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4 degree over night)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).)

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Prdx1, Polyclonal Antibody (Cat# AAA319486)

Full Name

Prdx1 (Phospho-Tyr194) Antibody

Gene Names

PRDX1; PAG; PAGA; PAGB; PRX1; PRXI; MSP23; NKEFA; TDPX2; NKEF-A

Reactivity

Human, Rat, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Pricing

$300/0.05 mL $420/0.1 mL $660/0.2 mL $1,285/2x0.2 mL $1,875/3x0.2 mL $2,430/4x0.2 mL $2,735/5x0.2 mL

WB (Western Blot) (Western Blot analysis of various cells using Phospho-GSK3beta (S9) Polyclonal Antibody diluted at 1:500)

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GSK3beta, ELISA Kit (Cat# AAA319079)

Full Name

GSK3beta (Phospho-Ser9) Colorimetric Cell-Based ELISA Kit

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of various cell lysis. Primary Antibody was diluted at 1:1000. Secondary antibody(was diluted at 1:10000)

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ERalpha, ELISA Kit (Cat# AAA319082)

Full Name

ERalpha (Phospho-Ser102) Colorimetric Cell-Based ELISA Kit

Gene Names

ESR1; ER; ESR; Era; ESRA; NR3A1

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of Hela cell lysis treated or untreated by EGF 20ng/ml 30'.. Primary Antibody was diluted at 1:1000. Secondary antibody( was diluted at 1:10000)

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FoxM1, ELISA Kit (Cat# AAA319086)

Full Name

FoxM1 (Phospho-Ser35) Colorimetric Cell-Based ELISA Kit

Gene Names

FOXM1; MPP2; TGT3; HFH11; HNF-3; INS-1; MPP-2; PIG29; FKHL16; FOXM1B; HFH-11; TRIDENT; MPHOSPH2

Reactivity

Human, Rat, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of Rac1(Phospho-Ser71) antibody in 293 cells lysates, cell treated or untreated with PMA 125ng/ml 30', 4 degree C over night, secondary antibody(cat: SA0002 was diluted at 1:10000, 37 degree C 1hour.)

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Rac1/cdc42, ELISA Kit (Cat# AAA319088)

Full Name

Rac1/cdc42 (Phospho-Ser71) Colorimetric Cell-Based ELISA Kit

Gene Names

CDC42; G25K; CDC42Hs

Reactivity

Human, Rat, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4 degree C over night)

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LDHA, ELISA Kit (Cat# AAA319093)

Full Name

LDHA (Phospho-Tyr10) Colorimetric Cell-Based ELISA Kit

Gene Names

LDHA; LDH1; LDHM; GSD11; PIG19

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4 degree C over night)

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CDC37, ELISA Kit (Cat# AAA319097)

Full Name

CDC37 (Phospho-Ser13) Colorimetric Cell-Based ELISA Kit

Gene Names

CDC37; P50CDC37

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of extracts from Hela cells, 1:2000. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).)

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Histone H2A.X, ELISA Kit (Cat# AAA319108)

Full Name

Histone H2A.X (Phospho-Tyr142) Colorimetric Cell-Based ELISA Kit

Gene Names

H2AFX; H2AX; H2A.X; H2A/X

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from KB cells, primary antibody was diluted at 1:1000, 4 degree C over night)

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NPM, ELISA Kit (Cat# AAA319114)

Full Name

NPM (Phospho-Ser4) Colorimetric Cell-Based ELISA Kit

Gene Names

NPM1; B23; NPM

Reactivity

Human, Rat, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from DU145 cells, primary antibody was diluted at 1:1000, 4 degree C over night)

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SEK1/MKK4, ELISA Kit (Cat# AAA319121)

Full Name

SEK1/MKK4 (Phospho-Ser257) Colorimetric Cell-Based ELISA Kit

Gene Names

MAP2K4; JNKK; MEK4; MKK4; SEK1; JNKK1; SERK1; MAPKK4; PRKMK4; SAPKK1; SAPKK-1

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of various cell lysis(provided by our customer). Primary Antibody was diluted at 1:1000. Secondary antibody(was diluted at 1:10000)

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ULK1, ELISA Kit (Cat# AAA319132)

Full Name

ULK1 (Phospho-Ser757) Colorimetric Cell-Based ELISA Kit

Gene Names

ULK1; ATG1; ATG1A; UNC51; hATG1; Unc51.1

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of HELA cells using Antibody diluted at 500. Secondary antibody was diluted at 1:20000)

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Vimentin, ELISA Kit (Cat# AAA319133)

Full Name

Vimentin (Phospho-Tyr38) Colorimetric Cell-Based ELISA Kit

Reactivity

Human: Y38, Mouse: Y38, Rat: Y38

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4 degree C over night)

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Stathmin, ELISA Kit (Cat# AAA319135)

Full Name

Stathmin (Phospho-Ser38) Colorimetric Cell-Based ELISA Kit

Gene Names

STMN2; SCG10; SCGN10

Reactivity

Human, Rat, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from Jurkat cells, using Catalase (Phospho-Tyr385) Antibody. The lane on the right is blocked with the phospho peptide.)

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Catalase, ELISA Kit (Cat# AAA319136)

Full Name

Catalase (Phospho-Tyr386) Colorimetric Cell-Based ELISA Kit

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4 degree C over night)

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Mcl-1, ELISA Kit (Cat# AAA319139)

Full Name

Mcl-1 (Phospho-Thr163) Colorimetric Cell-Based ELISA Kit

Gene Names

MCL1; TM; EAT; MCL1L; MCL1S; Mcl-1; BCL2L3; MCL1-ES; bcl2-L-3; mcl1/EAT

Reactivity

Human, Rat, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of various cells using Phospho-Akt1 (T450) Polyclonal Antibody diluted at 1:1000)

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Akt1, ELISA Kit (Cat# AAA319157)

Full Name

Akt1 (Phospho-Thr450) Colorimetric Cell-Based ELISA Kit

Gene Names

AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of 293 cells using Phospho-MSK2 (T568) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).)

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MSK2, ELISA Kit (Cat# AAA319258)

Full Name

MSK2 (Phospho-Thr568) Colorimetric Cell-Based ELISA Kit

Gene Names

RPS6KA4; MSK2; RSK-B

Reactivity

Human, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from K562 cells, using CD171/N-CAML1 (Phospho-Ser1181) Antibody. The lane on the right is blocked with the phospho peptide.)

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NCAM-L1, ELISA Kit (Cat# AAA319261)

Full Name

NCAM-L1 (Phospho-Ser1181) Colorimetric Cell-Based ELISA Kit

Gene Names

L1CAM; S10; HSAS; MASA; MIC5; SPG1; CAML1; CD171; HSAS1; N-CAML1; NCAM-L1; N-CAM-L1

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of HepG2 cells using Phospho-Nur77 (S351) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).)

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Nur77, ELISA Kit (Cat# AAA319266)

Full Name

Nur77 (Phospho-Ser351) Colorimetric Cell-Based ELISA Kit

Gene Names

NR4A1; HMR; N10; TR3; NP10; GFRP1; NAK-1; NGFIB; NUR77

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of VEC using p-p47-phox (S370) antibody.)

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p47-phox, ELISA Kit (Cat# AAA319268)

Full Name

p47-phox (Phospho-Ser370) Colorimetric Cell-Based ELISA Kit

Gene Names

NCF1; NCF1A; NOXO2; p47phox; SH3PXD1A

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of various cells using Phospho-PDGFR-beta (Y1021) Polyclonal Antibody)

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PDGFR-alpha, ELISA Kit (Cat# AAA319272)

Full Name

PDGFR-alpha (Phospho-Tyr1021) Colorimetric Cell-Based ELISA Kit

Gene Names

PDGFRB; IBGC4; JTK12; PDGFR; CD140B; PDGFR1; PDGFR-1

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of PP1-alpha (Phospho-Thr320) Antibody. The lane on the right is blocked with the PP1-alpha (Phospho-Thr320) peptide.)

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PP1alpha, ELISA Kit (Cat# AAA319275)

Full Name

PP1alpha (Phospho-Thr320) Colorimetric Cell-Based ELISA Kit

Gene Names

PPP1CA; PP1A; PP-1A; PPP1A; PP1alpha

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of MOUSE-LIVER cells using Phospho-TGFbeta RI (S165) Polyclonal Antibody diluted at 1:1000)

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TGFbeta RI, ELISA Kit (Cat# AAA319280)

Full Name

TGFbeta RI (Phospho-Ser165) Colorimetric Cell-Based ELISA Kit

Gene Names

TGFBR1; AAT5; ALK5; MSSE; SKR4; ALK-5; LDS1A; LDS2A; TGFR-1; ACVRLK4

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of COLO cells using Phospho-VDR (S51) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).)

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VDR, ELISA Kit (Cat# AAA319283)

Full Name

VDR (Phospho-Ser51) Colorimetric Cell-Based ELISA Kit

Gene Names

VDR; NR1I1

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of various cells using Phospho-Btk (Y551) Polyclonal Antibody)

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Btk, ELISA Kit (Cat# AAA319288)

Full Name

Btk (Phospho-Tyr551) Colorimetric Cell-Based ELISA Kit

Gene Names

BTK; AT; ATK; BPK; XLA; IMD1; AGMX1; PSCTK1

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of Rat-brain cells using Phospho-IP3R-I (S1764) Polyclonal Antibody)

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IP3R-I, ELISA Kit (Cat# AAA319289)

Full Name

IP3R-I (Phospho-Ser1764) Colorimetric Cell-Based ELISA Kit

Gene Names

ITPR1; IP3R; IP3R1; SCA15; SCA16; SCA29; INSP3R1

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

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WB (Western Blot) (Western blot analysis of Phospho-Retinoblastoma (Ser795) expression in various lysates)

IHC (Immunohistochemisry) (AAA321484 at 1/200 staining human brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IF (Immunofluorescence) (AAA321484 staining MOLT by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of Retinoblastoma phosphorylation expression in serum treated K562 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

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Retinoblastoma, Polyclonal Antibody (Cat# AAA321484)

Full Name

Phospho-Retinoblastoma (Ser795) Antibody

Gene Names

RB1; RB; pRb; OSRC; pp110; p105-Rb; PPP1R130

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IF (Immunofluorescence) (AAA321535 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of Phospho-RFA2 (Thr21) expression in various lysates)

IHC (Immunohiostchemistry) (AAA321535 at 1/200 staining human brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of RFA2 phosphorylation expression in Adriamycin treated HeLa whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

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RFA2, Polyclonal Antibody (Cat# AAA321535)

Full Name

Phospho-RFA2 (Thr21) Antibody

Gene Names

RPA2; REPA2; RPA32; RP-A p32; RP-A p34

Reactivity

Human, Mouse

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IF (Immunofluorescence) (AAA321579 staining K562 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

IHC (Immunohistochemisry) (AAA321579 at 1/200 staining human kidney carcinoma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of MKP-1/2 phosphorylation expression in heatshockK562 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

WB (Western Blot) (Western blot analysis of Phospho-MKP-1/2 (Ser296/318) Antibody expression in heatshock treated K562 cells lysates.The lane on the right is treated with the antigen-specific peptide.)

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MKP-1/2, Polyclonal Antibody (Cat# AAA321579)

Full Name

Phospho-MKP-1/2 (Ser296/318) Antibody

Gene Names

DUSP1; HVH1; MKP1; CL100; MKP-1; PTPN10

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of Phospho-Catenin beta (Tyr489)expression in various lysates)

WB (Western Blot) (Western blot analysis of Catenin-beta phosphorylation expression in UV treated COS7 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

IHC (Immunohistochemisry) (AAA321596 at 1/100 staining Mouse colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IF (Immunofluorescence) (AAA321596 staining SW626 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of Phospho-Catenin beta (Tyr489) Antibody expression in mouse kidney and rat liver tissues lysates.)

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Catenin beta, Polyclonal Antibody (Cat# AAA321596)

Full Name

Phospho-Catenin beta (Tyr489) Antibody

Gene Names

CTNNB1; EVR7; CTNNB; MRD19; armadillo

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of extracts of pc-3 cells treated with Calyculin A, using Phospho-KIF1B (Ser1487) Antibody.)

IHC (Immunohistochemistry) (AAA321341 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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KIF1B, Polyclonal Antibody (Cat# AAA321341)

Full Name

Phospho-KIF1B (Ser1487) Antibody

Gene Names

KIF1B; KLP; CMT2; CMT2A; CMT2A1; HMSNII; NBLST1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IF (Immunofluorescence) (AAA321438 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of p95/NBS1 phosphorylation expression in Jurkat whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

WB (Western Blot) (Western blot analysis of Phospho-p95/NBS1 (Ser343) expression in various lysates)

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p95/NBS1, Polyclonal Antibody (Cat# AAA321438)

Full Name

Phospho-p95/NBS1 (Ser343) Antibody

Gene Names

NBN; ATV; NBS; P95; NBS1; AT-V1; AT-V2

Reactivity

Human, Rat

Applications

Immunocytochemistry, Immunofluorescence, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IF (Immunofluorescence) (Glypican genes dally and dlp were required for JAK/STAT signaling activity and Upd distribution. A-B: dally was expressed in the eye discs in both 2nd instar larval stage (A-A") and early 3rd instar larval stage (B-B"). Expression of dally was marked by dally-lacZ (red in A' and B'), and overlapped with pSTAT staining (green in A-B).(References: Yan Zhang. ect. Drosophila glypicans Dally and Dally-like are essential regulators for JAK/STAT signaling and Unpaired distribution in eye development. Dev Biol. 2013 Mar 1;375(1):23-32. doi: 10.1016/j.ydbio.2012.12.019. Epub 2013 Jan 8.))

WB (Western Blot) (The levels of pStat92E and Stat92E are diminished in p115-depleted GSCs. (D) Western blot analysis and quantification of the levels of pStat92E protein in adult testes with indicated genotypes. b-Tubulin was used as blotting control. Data are shown as mean ± SD (n = 3). Two-tailed Student's t test was used. *p < 0.05.(References: Ruiyan Kong. ect. A feedforward loop between JAK/STAT downstream target p115 and STAT in germline stem cells. Stem Cell Reports. 2023 Oct 10;18(10):1940-1953. doi: 10.1016/j.stemcr.2023.08.007. Epub 2023 Sep 7.))

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Phospho-STAT92E (Y711), Antibody (Cat# AAA62492)

Full Name

Phospho-STAT92E (Y711) Antibody

Reactivity

Drosophila melanogaster (Fruit fly)

Applications

Immunofluorescence, Western Blot

Pricing

$445/0.05 mL $705/0.1 mL $1,375/2x0.1 mL $2,005/3x0.1 mL $2,605/4x0.1 mL

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DB (Dot Blot) (AbFlex ATM phospho Ser1981 antibody specifity Dot blot analysis was used to confirm the specificity of ATM phospho Ser1981 antibody. Single-stranded DNA oligonucleotides (amount of oligo in nanograms listed on the left side of the blot) were spotted on to a positively charged nylon membrane and blotted with antibody (0.5 ug/ml dilution). 1: ATM phospho serine 1981 (SLAFEEG(Sph)QSTTISS) 2: H2AX phospho serine 139 (CKATQA[Sph]QEY) 3: ATM unmodified serine 1981 (SLAFEEGSQSTTISS) 4: NBS phospho serine 343 (CPGPSL[Sph]QGVS) 5: BRCA1 phospho serine 1423 (LEQHG[Sph]QSNSC))

WB (Western Blot) (AbFlex ATM phospho Ser1981 antibody (rAb) tested by Western blot. 20 ug HeLa nuclear extract (high salt preparation) untreated (lane 1) or treated with VP16 at 100 uM for 8 hrs (Lane 2), probed with 1 ug/ml ATM phospho Ser1981 antibody. For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.)

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ATM phospho Ser1981, Antibody (Cat# AAA60273)

Full Name

AbFlex ATM phospho Ser1981 antibody (rAb)

Reactivity

Human, Mouse

Applications

Western Blot, Dot Blot

Purity

Protein A Chromatography

Pricing

$205/0.01 mg $575/0.1 mg $1,110/2x0.1 mg $1,620/3x0.1 mg $2,105/4x0.1 mg $2,395/5x0.1 mg

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Application Data

IHC (Immunohistochemistry)

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Raf1, Polyclonal Antibody (Cat# AAA47790)

Full Name

Raf1 (Phospho-Ser259)

Gene Names

RAF1; NS5; CRAF; Raf-1; c-Raf; CMD1NN

Applications

Immunohistochemistry, Western Blot

Pricing

$475/0.1 mL $925/2x0.1 mL $1,355/3x0.1 mL $1,755/4x0.1 mL $1,980/5x0.1 mL

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WB (Western Blot) (RNA pol II phospho Ser2 antibody (mAb) tested by Western blot. HeLa nuclear extract (20 ug per lane) probed with RNA pol II phospho Ser2 antibody (mAb) at a 1 ug/ml dilution.)

IF (Immunofluorescence) (Detection of RNA Pol II pS2 by immunofluorescence. U2OS cells were stained with RNA Pol II pS2 antibody at a dilution of 1:500. Left panel: DAPI. Middle panel: RNA Pol II pS2 antibody staining. Right panel: merge.)

ChIP (Chromatin Immunoprecipitation) (RNA pol II CTD phospho Ser2 antibody (mAb) tested by ChIP-Seq. ChIP-Seq was performed in a bladder cancer cell line using RNA pol II CTD phospho Ser5 antibody and RNA pol II CTD phospho Ser2 antibody . The average ChIP-Seq signal across all genes is shown in the graphic. As expected Pol II phosphoserine 5 is enriched at promoters and phosphoserine 2 is enriched toward the 3’ end of genes.)

ChIP (Chromatin Immunoprecipitation) (RNA pol II CTD phospho Ser2 antibody (mAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with chromatin from 2.3 million HL-60 cells and 10 ug of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 22 million sequence tags were mapped to identify RNA pol II phospho Ser2 binding. Data is compared to ChIP-Seq data using a phospho Ser5 antibody (61085). ChIP-Seq data from three specific genes is shown as an example. The Pol II phospho Ser2 antibody detects polymerase more toward the 3´ end of the genes and the phospho Ser5 antibody detects Pol II more at the 5´ end of the genes.)

ChIP (Chromatin Immunoprecipitation) (RNA pol II CTD phospho Ser2 antibody (mAb) tested by ChIP. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 10 ug of chromatin from human myeloma LP1 cells and 20 ug RNA pol II CTD phospho Ser2 antibody. ChIP DNA was used in qPCR with the control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.)

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RNA pol II CTD phospho Ser2, Monoclonal Antibody (Cat# AAA59974)

Full Name

RNA pol II CTD phospho Ser2 antibody (mAb)(Clone 3E10)

Gene Names

POLR2A; RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220

Reactivity

Human, Mouse

Applications

Western Blot, Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Chromatin immunoprecipitation, Chromatin immunoprecipitation

Purity

Protein G Chromatography

Pricing

$250/0.01 mg $455/0.05 mg $725/0.1 mg $1,415/2x0.1 mg $2,070/3x0.1 mg $2,680/4x0.1 mg $3,100/5x0.1 mg

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IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

DB (Dot Blot) (Dot blot analysis of anti-Phospho-ABL1-Y134 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

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Phospho-ABL1 (Y134), Polyclonal Antibody (Cat# AAA288054)

Full Name

Phospho-ABL1 (Y134) Antibody

Gene Names

ABL1; ABL; JTK7; p150; c-ABL; v-abl; c-ABL1; bcr/abl

Reactivity

Human (Predicted Reactivity: Mouse)

Applications

Immunohistochemistry, Dot Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human lymph node tissue using IkappaB-alpha (Phospho-Tyr305) antibody.)

WB (Western Blot) (Western blot analysis of extracts from COS7 cells, treated with nocodazole (1ug/ml, 16hours), using IkappaB-alpha (Phospho-Tyr305) antibody.)

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IkappaB-alpha (Phospho-Tyr305), Polyclonal Antibody (Cat# AAA289645)

Full Name

IkappaB-alpha (Phospho-Tyr305) Antibody

Gene Names

NFKBIA; IKBA; MAD-3; NFKBI

Reactivity

Human, mouse, rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

Application Data (The TFact B-Myb (Phospho-Ser577/581) DNA-Binding ELISA detects active B-Myb (Phospho-Ser577/581) in HepG2 and MCF7 Nuclear Extract. The HepG2 and MCF7 cells were grown 3 days in DMEM with 10% FBS and harvested for nuclear extract.)

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B-Myb, ELISA Kit (Cat# AAA316029)

Full Name

B-Myb (Phospho-Ser577/581) TFact DNA-Binding ELISA Kit

Gene Names

MYBL2; BMYB; B-MYB

Reactivity

Human, Mouse, Rat

Pricing

$635/1 Kit $1,230/2x1 Kit $1,800/3x1 Kit $2,335/4x1 Kit $2,905/5x1 Kit

Application Data (The TFact c-Jun DNA-Binding ELISA kits detect active c-Jun in Hela Nuclear Extract. The Hela cells were grown 3 days in DMEM with 10% FBS and harvested for nuclear extract. The Hela cells were stimulated by UV (100J/M2) before harvest.)

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c-Jun, ELISA Kit (Cat# AAA315850)

Full Name

c-Jun (Phospho-Ser63) TFact DNA-Binding ELISA Kit

Gene Names

JUN; AP1; p39; AP-1; c-Jun

Reactivity

Human, Mouse, Rat

Pricing

$635/1 Kit $1,230/2x1 Kit $1,800/3x1 Kit $2,335/4x1 Kit $2,905/5x1 Kit

What Are Phospho Antibodies?

Protein phosphorylation is a process where a phosphate group is added to certain amino acid residues of a protein – usually serine (S), threonine (T), or tyrosine (Y) - by enzymes called kinases. This process is integral in controlling cellular signaling, cellular growth, and other biological functions.

Our catalog includes a wide range of phospho-specific antibodies that can accurately detect this important marker. They perform strongly in widely-used laboratory applications such as Western blot, flow cytometry, immunohistochemistry, and immunofluorescence microscopy. We value your trust in us and are committed to providing top-quality products and services. All of our antibodies are guaranteed to work for the applications and species indicated on our website & associated product pages.

What Are The Key Applications of Phospho Antibodies?

1. Western Blotting

One of the first steps a researcher can take in utilizing these phospho-specific antibodies, is to check if the antibody works using a technique referred to as “Western blot”. For those unfamiliar, Western Blot aids in showing whether the protein that the antibody recognizes is appearing at the correct/expected size. These phospho-specific antibodies should also be able to detect changes in the target protein’s phosphorylation (on/off state) when cells are stimulated in certain ways.

2. Staining of Fixed Cells (Immunocytochemistry)

Another routine use of these phospho-specific antibodies, is to test if the antibody is able to demonstrate similar performance when used on fixed cells (intact cells that have been preserved) as it did in the Western blot tests. It is an important aspect in many cases to confirm that the antibody works in actual intact cell samples. Ideally, the method used for cellular fixation should be the same as what is used in pathology labs (like using 10% formalin). To check if the antibody works well in tissue sections (FFPE), researchers will often test it on fixed cells that are processed similar to tissue samples.

3. Specificity Tests Using Peptides

In order to make sure that the antibody is only binding to the right target:

• Laboratory technicians will mix the antibody with phospho-peptides (short segments of the protein containing the phosphate group modification).


• If the antibody signal disappears, it is confirmation that it is binding to the correct phosphorylated location.


• A more robust test is to use both the phosphorylated and non-phosphorylated (dephosphorylated) versions of the protein. The antibody should react only with the phosphorylated one.


• Another method sometimes utilized is to treat the sample with an enzyme, such as alkaline phosphatase, that specifically removes phosphate groups. If the antibody signal disappears after this, it also confirms specificity.

4. Genetic Confirmation

As a final step, scientists can genetically manipulate the nucleotide sequence and alter the target protein by removing the exact site where phosphorylation happens. If the antibody no longer appears to detect the modified protein, it is strong evidence supporting the antibody being specific for that phosphorylated site.

Why Buy Phospho Antibodies Through Us?

• The production laboratory adheres to strict and consistent protocols prior to releasing any of these phospho-specific antibodies:


• Standard methods and proper controls in all tests to ensure high quality.


• These antibodies are tested and validated in different cell types and species.


• High quality control criterion to ensure each batch is consistent, so you will obtain reliable results every time.

FAQ

1. What Are Phospho-Specific Antibodies?

Phospho-specific antibodies are made to detect proteins only when they have a phosphate group linked to a specific amino acid residue. This empowers scientists understand if a protein is "turned on" or active, based on its phosphorylation state.

2. How to Detect Phosphorylated Proteins in a Western Blot?

To find out if a protein is phosphorylated using Western blot:

• Use a phospho-specific antibody that binds only to the phosphorylated form of the protein.


• You can also use a “regular” antibody for the same amino acid sequence of the protein that the phospho-specific antibody is binding to (but in this case, this antibody will not bind if there is a phosphate group present) in order to compare how much of it is phosphorylated versus how much is non-phosphorylated (or “total” protein, if the “normal” antibody’s epitopes are non-phospho-site-specific).

3. How to Choose the Best Antibody?

Here are some simple tips to help you pick the right antibody:

• Know your target


• Match your sample characteristics


• Confirm the intended use is appropriate


• Check “host” and “type”


• Check the “quality” of the presented data/images


• Appraise whether the available validation meets your needs