Phospho Antibodies

Phospho-specific antibodies’ typical purpose is to enable researchers to detect changes in proteins. They will exclusively bind to the amino acid sequence on a protein that has been phosphorylated (which is both a physical & chemical change) and do not bind to the same amino acid sequence on said protein if it lacks said phosphorylation. This aids in being able to clearly see and understand the data produced from this particular protein modification.

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ELISA (Enzyme-Linked Immunosorbent Assay (ELISA) for immunogen phosphor-peptide (left) and non-phospho peptide (right), using Anti-Myc (Phospho-Ser62) Antibody.)

Application Data (HeLa cells were grown to 90% confluency and were stimulated with EGF 200ng/mL for 30 minutes. Cells were immediately lysed thereafter and measured for Total Protein Concentration and O.D. 450 nm of Myc P-Ser20 versus untreated HeLa lysates.)

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Myc, ELISA Kit (Cat# AAA318611)

Full Name

Myc (Phospho-Ser62) Phospho Sandwich ELISA Kit

Gene Names

MYC; MRTL; MYCC; c-Myc; bHLHe39

Reactivity

Human, Mouse, Rat

Pricing

$540/1 Kit $1,040/2x1 Kit $1,515/3x1 Kit $1,965/4x1 Kit $2,445/5x1 Kit

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ELISA (Enzyme-Linked Immunosorbent Assay (ELISA) for immunogen phosphor-peptide (left) and non-phospho peptide (right), using Anti-GSK3 beta (Phospho-Ser9) Antibody.)

Application Data (HeLa cells were grown to 90% confluency and were stimulated with EGF 200 ng/mL for 30 minutes. Cells were immediately lysed thereafter and measured for Total Protein Concentration and O.D. 450 nm of GSK3 beta P-Ser9 versus untreated HeLa lysates.)

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GSK3 beta, ELISA Kit (Cat# AAA318624)

Full Name

GSK3 beta (Phospho-Ser9) Phospho Sandwich ELISA Kit

Reactivity

Human, Mouse, Rat

Pricing

$540/1 Kit $1,040/2x1 Kit $1,515/3x1 Kit $1,965/4x1 Kit $2,445/5x1 Kit

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IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded Human tonsil. Antibody was diluted at 1:100(4 degree overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast cancer, using VASP (Phospho-Ser157) Antibody. The picture on the right is blocked with the VASP (Phospho-Ser157) peptide.)

WB (Western Blot) (Western blot analysis of VASP (Phospho-Ser157) Antibody. The lane on the right is blocked with the VASP (Phospho-Ser157) peptide.)

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VASP, Polyclonal Antibody (Cat# AAA318644)

Full Name

VASP (Phospho-Ser157) Rabbit Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).)

WB (Western Blot) (Western Blot analysis of 1 Hela, 2 treated with LPS 100ng/mL 20mim,using primary antibody at 1:1000 dilution. Secondary antibody)

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PC-PLD1, Polyclonal Antibody (Cat# AAA318656)

Full Name

PC-PLD1 (Phospho-Thr147) Rabbit Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

WB (Western Blot) (Western blot analysis of LOVO using p-p70 S6 kinase alpha (T444) antibody. Antibody was diluted at 1:500)

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p70 S6 kinase alpha, ELISA Kit (Cat# AAA319185)

Full Name

p70 S6 kinase alpha (Phospho-Thr444) Colorimetric Cell-Based ELISA Kit

Gene Names

RPS6KB1; S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of 293T AD293 Hela using p-Parkin (S131) antibody. Antibody was diluted at 1:500)

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Parkin, ELISA Kit (Cat# AAA319187)

Full Name

Parkin (Phospho-Ser131) Colorimetric Cell-Based ELISA Kit

Gene Names

PARK2; PDJ; PRKN; AR-JP; LPRS2

Reactivity

Human, Rat, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from COLO205 cells, using STK39 (Phospho-Ser311) Antibody. The lane on the right is blocked with the phospho peptide.)

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SPAK, ELISA Kit (Cat# AAA319195)

Full Name

SPAK (Phospho-Ser309) Colorimetric Cell-Based ELISA Kit

Gene Names

STK39; DCHT; PASK; SPAK

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of various cells using Phospho-Synuclein-alpha (Y125) Polyclonal Antibody)

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Synuclein-alpha, ELISA Kit (Cat# AAA319198)

Full Name

Synuclein-alpha (Phospho-Tyr125) Colorimetric Cell-Based ELISA Kit

Gene Names

SNCA; PD1; NACP; PARK1; PARK4

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of various cells using Phospho-Akt (T308) Polyclonal Antibody diluted at 1:1000)

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Akt, ELISA Kit (Cat# AAA319209)

Full Name

Akt (Phospho-Thr308) Colorimetric Cell-Based ELISA Kit

Gene Names

AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA

Reactivity

Human, Mouse, Rat, Monkey

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of various cells using Phospho-Cdc25C (S216) Polyclonal Antibody diluted at 1:1000)

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Cdc25C, ELISA Kit (Cat# AAA319215)

Full Name

Cdc25C (Phospho-Ser216) Colorimetric Cell-Based ELISA Kit

Gene Names

CDC25C; CDC25; PPP1R60

Reactivity

Human, Rat, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of JK cells using Phospho-DRP1 (S637) Polyclonal Antibody)

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DRP1, ELISA Kit (Cat# AAA319225)

Full Name

DRP1 (Phospho-Ser637) Colorimetric Cell-Based ELISA Kit

Gene Names

DNM1L; DLP1; DRP1; DVLP; EMPF; DYMPLE; HDYNIV

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of HepG2 cells using Phospho-Flg/Bek (Y463/466) Polyclonal Antibody)

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Flg/Bek, ELISA Kit (Cat# AAA319230)

Full Name

Flg/Bek (Phospho-Tyr463/466) Colorimetric Cell-Based ELISA Kit

Gene Names

FGFR1; CEK; FLG; HH2; OGD; FLT2; KAL2; BFGFR; CD331; FGFBR; FLT-2; HBGFR; N-SAM; FGFR-1; bFGF-R-1

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of Hela+UV 5' cells using Phospho-Histone H2A (T121) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).)

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Histone H2A, ELISA Kit (Cat# AAA319235)

Full Name

Histone H2A (Phospho-Thr121) Colorimetric Cell-Based ELISA Kit

Gene Names

HIST1H2AI; H2A/c; H2AFC

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of VEC using p-HSP90beta (S226) antibody.)

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HSP90beta, ELISA Kit (Cat# AAA319239)

Full Name

HSP90beta (Phospho-Ser226) Colorimetric Cell-Based ELISA Kit

Gene Names

HSP90AB1; HSP84; HSPC2; HSPCB; D6S182; HSP90B

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from RAW264.7 cells treated with forskolin 40nM 30', using Kir5.1 (Phospho-Ser417) Antibody. The lane on the right is blocked with the phospho peptide.)

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KIR5.1, ELISA Kit (Cat# AAA319248)

Full Name

KIR5.1 (Phospho-Ser416) Colorimetric Cell-Based ELISA Kit

Gene Names

Kcnj16; Kir5.1; AI132396; 6430410F18Rik

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of 293 cells using Phospho-MSK2 (T568) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).)

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MSK2, ELISA Kit (Cat# AAA319258)

Full Name

MSK2 (Phospho-Thr568) Colorimetric Cell-Based ELISA Kit

Gene Names

RPS6KA4; MSK2; RSK-B

Reactivity

Human, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of extracts from Hela cells, untreated (-) or treated, 1:5000. Secondary antibody was diluted at 1:20000)

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Histone H1, ELISA Kit (Cat# AAA319064)

Full Name

Histone H1 (Phospho-Ser1) Colorimetric Cell-Based ELISA Kit

Gene Names

HIST1H1A; H1A; H1.1; H1F1; HIST1

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of extracts from Hela cells, untreated (-) or treated, 1:2000. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).)

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Histone H3, ELISA Kit (Cat# AAA319068)

Full Name

Histone H3 (Phospho-Ser28) Colorimetric Cell-Based ELISA Kit

Gene Names

HIST1H3A; H3/A; H3FA

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of extracts from Hela cells, untreated (-) or treated, 1:2000. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).)

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Histone H3, ELISA Kit (Cat# AAA319072)

Full Name

Histone H3 (Phospho-Thr32) Colorimetric Cell-Based ELISA Kit

Gene Names

HIST1H3A; H3/A; H3FA

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of extracts from Hela cells, untreated (-) or treated, 1:2000. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).)

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Histone H3, ELISA Kit (Cat# AAA319074)

Full Name

Histone H3 (Phospho-Thr6) Colorimetric Cell-Based ELISA Kit

Gene Names

HIST1H3A; H3/A; H3FA

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of extracts from Hela cells, untreated (-) or treated, 1:2000. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).)

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Histone H3, ELISA Kit (Cat# AAA319075)

Full Name

Histone H3 (Phospho-Tyr41) Colorimetric Cell-Based ELISA Kit

Gene Names

HIST1H3A; H3/A; H3FA

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of various cells using Phospho-GSK3beta (S9) Polyclonal Antibody diluted at 1:500)

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GSK3beta, ELISA Kit (Cat# AAA319079)

Full Name

GSK3beta (Phospho-Ser9) Colorimetric Cell-Based ELISA Kit

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of various cell lysis. Primary Antibody was diluted at 1:1000. Secondary antibody(was diluted at 1:10000)

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ERalpha, ELISA Kit (Cat# AAA319082)

Full Name

ERalpha (Phospho-Ser102) Colorimetric Cell-Based ELISA Kit

Gene Names

ESR1; ER; ESR; Era; ESRA; NR3A1

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of Hela cell lysis treated or untreated by EGF 20ng/ml 30'.. Primary Antibody was diluted at 1:1000. Secondary antibody( was diluted at 1:10000)

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FoxM1, ELISA Kit (Cat# AAA319086)

Full Name

FoxM1 (Phospho-Ser35) Colorimetric Cell-Based ELISA Kit

Gene Names

FOXM1; MPP2; TGT3; HFH11; HNF-3; INS-1; MPP-2; PIG29; FKHL16; FOXM1B; HFH-11; TRIDENT; MPHOSPH2

Reactivity

Human, Rat, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of Rac1(Phospho-Ser71) antibody in 293 cells lysates, cell treated or untreated with PMA 125ng/ml 30', 4 degree C over night, secondary antibody(cat: SA0002 was diluted at 1:10000, 37 degree C 1hour.)

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Rac1/cdc42, ELISA Kit (Cat# AAA319088)

Full Name

Rac1/cdc42 (Phospho-Ser71) Colorimetric Cell-Based ELISA Kit

Gene Names

CDC42; G25K; CDC42Hs

Reactivity

Human, Rat, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4 degree C over night)

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LDHA, ELISA Kit (Cat# AAA319093)

Full Name

LDHA (Phospho-Tyr10) Colorimetric Cell-Based ELISA Kit

Gene Names

LDHA; LDH1; LDHM; GSD11; PIG19

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4 degree C over night)

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CDC37, ELISA Kit (Cat# AAA319097)

Full Name

CDC37 (Phospho-Ser13) Colorimetric Cell-Based ELISA Kit

Gene Names

CDC37; P50CDC37

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of extracts from Hela cells, 1:2000. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).)

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Histone H2A.X, ELISA Kit (Cat# AAA319108)

Full Name

Histone H2A.X (Phospho-Tyr142) Colorimetric Cell-Based ELISA Kit

Gene Names

H2AFX; H2AX; H2A.X; H2A/X

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from KB cells, primary antibody was diluted at 1:1000, 4 degree C over night)

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NPM, ELISA Kit (Cat# AAA319114)

Full Name

NPM (Phospho-Ser4) Colorimetric Cell-Based ELISA Kit

Gene Names

NPM1; B23; NPM

Reactivity

Human, Rat, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from DU145 cells, primary antibody was diluted at 1:1000, 4 degree C over night)

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SEK1/MKK4, ELISA Kit (Cat# AAA319121)

Full Name

SEK1/MKK4 (Phospho-Ser257) Colorimetric Cell-Based ELISA Kit

Gene Names

MAP2K4; JNKK; MEK4; MKK4; SEK1; JNKK1; SERK1; MAPKK4; PRKMK4; SAPKK1; SAPKK-1

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of various cell lysis(provided by our customer). Primary Antibody was diluted at 1:1000. Secondary antibody(was diluted at 1:10000)

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ULK1, ELISA Kit (Cat# AAA319132)

Full Name

ULK1 (Phospho-Ser757) Colorimetric Cell-Based ELISA Kit

Gene Names

ULK1; ATG1; ATG1A; UNC51; hATG1; Unc51.1

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of HELA cells using Antibody diluted at 500. Secondary antibody was diluted at 1:20000)

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Vimentin, ELISA Kit (Cat# AAA319133)

Full Name

Vimentin (Phospho-Tyr38) Colorimetric Cell-Based ELISA Kit

Reactivity

Human: Y38, Mouse: Y38, Rat: Y38

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4 degree C over night)

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Stathmin, ELISA Kit (Cat# AAA319135)

Full Name

Stathmin (Phospho-Ser38) Colorimetric Cell-Based ELISA Kit

Gene Names

STMN2; SCG10; SCGN10

Reactivity

Human, Rat, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from Jurkat cells, using Catalase (Phospho-Tyr385) Antibody. The lane on the right is blocked with the phospho peptide.)

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Catalase, ELISA Kit (Cat# AAA319136)

Full Name

Catalase (Phospho-Tyr386) Colorimetric Cell-Based ELISA Kit

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4 degree C over night)

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Mcl-1, ELISA Kit (Cat# AAA319139)

Full Name

Mcl-1 (Phospho-Thr163) Colorimetric Cell-Based ELISA Kit

Gene Names

MCL1; TM; EAT; MCL1L; MCL1S; Mcl-1; BCL2L3; MCL1-ES; bcl2-L-3; mcl1/EAT

Reactivity

Human, Rat, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of various cells using Phospho-Akt1 (T450) Polyclonal Antibody diluted at 1:1000)

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Akt1, ELISA Kit (Cat# AAA319157)

Full Name

Akt1 (Phospho-Thr450) Colorimetric Cell-Based ELISA Kit

Gene Names

AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of VEC using p-cPLA2 (S505) antibody. Antibody was diluted at 1:1000)

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cPLA2, ELISA Kit (Cat# AAA319164)

Full Name

cPLA2 (Phospho-Ser505) Colorimetric Cell-Based ELISA Kit

Gene Names

PLA2G4A; PLA2G4; cPLA2-alpha

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of various cells using Phospho-Cyclin E1 (T395) Polyclonal Antibody diluted at 1:2000)

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Cyclin E1, ELISA Kit (Cat# AAA319165)

Full Name

Cyclin E1 (Phospho-Thr395) Colorimetric Cell-Based ELISA Kit

Gene Names

CCNE1; CCNE

Reactivity

Human, Rat, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of Hela cells using Phospho-ErbB-3 (Y1289) Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).)

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ErbB-3, ELISA Kit (Cat# AAA319167)

Full Name

ErbB-3 (Phospho-Tyr1289) Colorimetric Cell-Based ELISA Kit

Gene Names

ERBB3; HER3; LCCS2; ErbB-3; c-erbB3; erbB3-S; MDA-BF-1; c-erbB-3; p180-ErbB3; p45-sErbB3; p85-sErbB3

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from K562 cells, using CD171/N-CAML1 (Phospho-Ser1181) Antibody. The lane on the right is blocked with the phospho peptide.)

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NCAM-L1, ELISA Kit (Cat# AAA319261)

Full Name

NCAM-L1 (Phospho-Ser1181) Colorimetric Cell-Based ELISA Kit

Gene Names

L1CAM; S10; HSAS; MASA; MIC5; SPG1; CAML1; CD171; HSAS1; N-CAML1; NCAM-L1; N-CAM-L1

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of HepG2 cells using Phospho-Nur77 (S351) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).)

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Nur77, ELISA Kit (Cat# AAA319266)

Full Name

Nur77 (Phospho-Ser351) Colorimetric Cell-Based ELISA Kit

Gene Names

NR4A1; HMR; N10; TR3; NP10; GFRP1; NAK-1; NGFIB; NUR77

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of VEC using p-p47-phox (S370) antibody.)

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p47-phox, ELISA Kit (Cat# AAA319268)

Full Name

p47-phox (Phospho-Ser370) Colorimetric Cell-Based ELISA Kit

Gene Names

NCF1; NCF1A; NOXO2; p47phox; SH3PXD1A

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of various cells using Phospho-PDGFR-beta (Y1021) Polyclonal Antibody)

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PDGFR-alpha, ELISA Kit (Cat# AAA319272)

Full Name

PDGFR-alpha (Phospho-Tyr1021) Colorimetric Cell-Based ELISA Kit

Gene Names

PDGFRB; IBGC4; JTK12; PDGFR; CD140B; PDGFR1; PDGFR-1

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of PP1-alpha (Phospho-Thr320) Antibody. The lane on the right is blocked with the PP1-alpha (Phospho-Thr320) peptide.)

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PP1alpha, ELISA Kit (Cat# AAA319275)

Full Name

PP1alpha (Phospho-Thr320) Colorimetric Cell-Based ELISA Kit

Gene Names

PPP1CA; PP1A; PP-1A; PPP1A; PP1alpha

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of MOUSE-LIVER cells using Phospho-TGFbeta RI (S165) Polyclonal Antibody diluted at 1:1000)

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TGFbeta RI, ELISA Kit (Cat# AAA319280)

Full Name

TGFbeta RI (Phospho-Ser165) Colorimetric Cell-Based ELISA Kit

Gene Names

TGFBR1; AAT5; ALK5; MSSE; SKR4; ALK-5; LDS1A; LDS2A; TGFR-1; ACVRLK4

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of COLO cells using Phospho-VDR (S51) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).)

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VDR, ELISA Kit (Cat# AAA319283)

Full Name

VDR (Phospho-Ser51) Colorimetric Cell-Based ELISA Kit

Gene Names

VDR; NR1I1

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of various cells using Phospho-Btk (Y551) Polyclonal Antibody)

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Btk, ELISA Kit (Cat# AAA319288)

Full Name

Btk (Phospho-Tyr551) Colorimetric Cell-Based ELISA Kit

Gene Names

BTK; AT; ATK; BPK; XLA; IMD1; AGMX1; PSCTK1

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of Rat-brain cells using Phospho-IP3R-I (S1764) Polyclonal Antibody)

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IP3R-I, ELISA Kit (Cat# AAA319289)

Full Name

IP3R-I (Phospho-Ser1764) Colorimetric Cell-Based ELISA Kit

Gene Names

ITPR1; IP3R; IP3R1; SCA15; SCA16; SCA29; INSP3R1

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

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WB (Western Blot) (Western blot analysis of Phospho-Retinoblastoma (Ser795) expression in various lysates)

IHC (Immunohistochemisry) (AAA321484 at 1/200 staining human brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IF (Immunofluorescence) (AAA321484 staining MOLT by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of Retinoblastoma phosphorylation expression in serum treated K562 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

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Retinoblastoma, Polyclonal Antibody (Cat# AAA321484)

Full Name

Phospho-Retinoblastoma (Ser795) Antibody

Gene Names

RB1; RB; pRb; OSRC; pp110; p105-Rb; PPP1R130

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IF (Immunofluorescence) (AAA321535 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of Phospho-RFA2 (Thr21) expression in various lysates)

IHC (Immunohiostchemistry) (AAA321535 at 1/200 staining human brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of RFA2 phosphorylation expression in Adriamycin treated HeLa whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

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RFA2, Polyclonal Antibody (Cat# AAA321535)

Full Name

Phospho-RFA2 (Thr21) Antibody

Gene Names

RPA2; REPA2; RPA32; RP-A p32; RP-A p34

Reactivity

Human, Mouse

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

What Are Phospho Antibodies?

Protein phosphorylation is a process where a phosphate group is added to certain amino acid residues of a protein – usually serine (S), threonine (T), or tyrosine (Y) - by enzymes called kinases. This process is integral in controlling cellular signaling, cellular growth, and other biological functions.

Our catalog includes a wide range of phospho-specific antibodies that can accurately detect this important marker. They perform strongly in widely-used laboratory applications such as Western blot, flow cytometry, immunohistochemistry, and immunofluorescence microscopy. We value your trust in us and are committed to providing top-quality products and services. All of our antibodies are guaranteed to work for the applications and species indicated on our website & associated product pages.

What Are The Key Applications of Phospho Antibodies?

1. Western Blotting

One of the first steps a researcher can take in utilizing these phospho-specific antibodies, is to check if the antibody works using a technique referred to as “Western blot”. For those unfamiliar, Western Blot aids in showing whether the protein that the antibody recognizes is appearing at the correct/expected size. These phospho-specific antibodies should also be able to detect changes in the target protein’s phosphorylation (on/off state) when cells are stimulated in certain ways.

2. Staining of Fixed Cells (Immunocytochemistry)

Another routine use of these phospho-specific antibodies, is to test if the antibody is able to demonstrate similar performance when used on fixed cells (intact cells that have been preserved) as it did in the Western blot tests. It is an important aspect in many cases to confirm that the antibody works in actual intact cell samples. Ideally, the method used for cellular fixation should be the same as what is used in pathology labs (like using 10% formalin). To check if the antibody works well in tissue sections (FFPE), researchers will often test it on fixed cells that are processed similar to tissue samples.

3. Specificity Tests Using Peptides

In order to make sure that the antibody is only binding to the right target:

• Laboratory technicians will mix the antibody with phospho-peptides (short segments of the protein containing the phosphate group modification).


• If the antibody signal disappears, it is confirmation that it is binding to the correct phosphorylated location.


• A more robust test is to use both the phosphorylated and non-phosphorylated (dephosphorylated) versions of the protein. The antibody should react only with the phosphorylated one.


• Another method sometimes utilized is to treat the sample with an enzyme, such as alkaline phosphatase, that specifically removes phosphate groups. If the antibody signal disappears after this, it also confirms specificity.

4. Genetic Confirmation

As a final step, scientists can genetically manipulate the nucleotide sequence and alter the target protein by removing the exact site where phosphorylation happens. If the antibody no longer appears to detect the modified protein, it is strong evidence supporting the antibody being specific for that phosphorylated site.

Why Buy Phospho Antibodies Through Us?

• The production laboratory adheres to strict and consistent protocols prior to releasing any of these phospho-specific antibodies:


• Standard methods and proper controls in all tests to ensure high quality.


• These antibodies are tested and validated in different cell types and species.


• High quality control criterion to ensure each batch is consistent, so you will obtain reliable results every time.

FAQ

1. What Are Phospho-Specific Antibodies?

Phospho-specific antibodies are made to detect proteins only when they have a phosphate group linked to a specific amino acid residue. This empowers scientists understand if a protein is "turned on" or active, based on its phosphorylation state.

2. How to Detect Phosphorylated Proteins in a Western Blot?

To find out if a protein is phosphorylated using Western blot:

• Use a phospho-specific antibody that binds only to the phosphorylated form of the protein.


• You can also use a “regular” antibody for the same amino acid sequence of the protein that the phospho-specific antibody is binding to (but in this case, this antibody will not bind if there is a phosphate group present) in order to compare how much of it is phosphorylated versus how much is non-phosphorylated (or “total” protein, if the “normal” antibody’s epitopes are non-phospho-site-specific).

3. How to Choose the Best Antibody?

Here are some simple tips to help you pick the right antibody:

• Know your target


• Match your sample characteristics


• Confirm the intended use is appropriate


• Check “host” and “type”


• Check the “quality” of the presented data/images


• Appraise whether the available validation meets your needs