Phospho Antibodies

Phospho-specific antibodies’ typical purpose is to enable researchers to detect changes in proteins. They will exclusively bind to the amino acid sequence on a protein that has been phosphorylated (which is both a physical & chemical change) and do not bind to the same amino acid sequence on said protein if it lacks said phosphorylation. This aids in being able to clearly see and understand the data produced from this particular protein modification.

WB (Western Blot) (Western Blot analysis of various cells using Phospho-ERK 1/2 (Y222/205) Polyclonal Antibody diluted at 1:500)

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ERK 1/2, ELISA Kit (Cat# AAA319227)

Full Name

ERK 1/2 (Phospho-Tyr222/205) Colorimetric Cell-Based ELISA Kit

Gene Names

MAPK1; ERK; p38; p40; p41; ERK2; ERT1; MAPK2; PRKM1; PRKM2; P42MAPK; p41mapk

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of 293 cells using Phospho-FAK (Y397) Polyclonal Antibody)

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FAK, ELISA Kit (Cat# AAA319228)

Full Name

FAK (Phospho-Tyr397) Colorimetric Cell-Based ELISA Kit

Gene Names

PTK2; FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of various cells using Phospho-Flt-1 (Y1048) Polyclonal Antibody diluted at 1:1000)

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Flt-1, ELISA Kit (Cat# AAA319231)

Full Name

Flt-1 (Phospho-Tyr1048) Colorimetric Cell-Based ELISA Kit

Gene Names

FLT1; FLT; FLT-1; VEGFR1; VEGFR-1

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of various cells using Phospho-Hrs (Y216) Polyclonal Antibody)

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Hrs, ELISA Kit (Cat# AAA319236)

Full Name

Hrs (Phospho-Tyr216) Colorimetric Cell-Based ELISA Kit

Gene Names

HGS; HRS

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from HepG2 cells, using IR (Phospho-Thr1375) Antibody. The lane on the right is blocked with the phospho peptide.)

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Insulin R, ELISA Kit (Cat# AAA319240)

Full Name

Insulin R (Phospho-Thr1375) Colorimetric Cell-Based ELISA Kit

Gene Names

INSR; HHF5; CD220

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4 degree C over night)

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IPP-2, ELISA Kit (Cat# AAA319241)

Full Name

IPP-2 (Phospho-Ser44) Colorimetric Cell-Based ELISA Kit

Gene Names

PPP1R2; IPP2; IPP-2

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from HeLa cells treated with calyculinA 50ng/ml 30', using MDM4 (Phospho-Ser367) Antibody. The lane on the right is blocked with the phospho peptide.)

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MDMX, ELISA Kit (Cat# AAA319253)

Full Name

MDMX (Phospho-Ser367) Colorimetric Cell-Based ELISA Kit

Gene Names

MDM4; HDMX; MDMX; MRP1

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of HELA Cell Lysate, antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000)

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p53, ELISA Kit (Cat# AAA319080)

Full Name

p53 (Phospho-Ser366) Colorimetric Cell-Based ELISA Kit

Gene Names

TP53; P53; BCC7; LFS1; TRP53

Reactivity

Human, Rat, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from HeLa cells treated with heat shock, using AMPK1 (Phospho-Ser485) Antibody. The lane on the right is blocked with the phospho peptide.)

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AMPKalpha1, ELISA Kit (Cat# AAA319081)

Full Name

AMPKalpha1 (Phospho-Ser485) Colorimetric Cell-Based ELISA Kit

Gene Names

PRKAA1; AMPK; AMPKa1

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4 degree C over night)

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NDRG1, ELISA Kit (Cat# AAA319084)

Full Name

NDRG1 (Phospho-Ser330) Colorimetric Cell-Based ELISA Kit

Gene Names

NDRG1; GC4; RTP; DRG1; NDR1; NMSL; TDD5; CAP43; CMT4D; DRG-1; HMSNL; RIT42; TARG1; PROXY1

Reactivity

Human, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from KB cells, primary antibody was diluted at 1:1000, 4 degree C over night)

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CD79A, ELISA Kit (Cat# AAA319096)

Full Name

CD79A (Phospho-Tyr182) Colorimetric Cell-Based ELISA Kit

Gene Names

CD79A; IGA; MB-1

Reactivity

Human, Rat, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from Jurkat cells, using phospho-CD318 (Phospho-Tyr707) antibody.)

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CD318, ELISA Kit (Cat# AAA319098)

Full Name

CD318 (Phospho-Tyr707) Colorimetric Cell-Based ELISA Kit

Gene Names

CDCP1; CD318; TRASK; SIMA135

Reactivity

Human, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4 degree C over night)

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cdc2, ELISA Kit (Cat# AAA319099)

Full Name

cdc2 (Phospho-Thr14) Colorimetric Cell-Based ELISA Kit

Gene Names

CDK1; CDC2; CDC28A; P34CDC2

Reactivity

Human, Rat, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of various cells using Phospho-Cdk2 (T160) Polyclonal Antibody diluted at 1:1000)

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Cdk2, ELISA Kit (Cat# AAA319100)

Full Name

Cdk2 (Phospho-Thr160) Colorimetric Cell-Based ELISA Kit

Gene Names

CDK2; p33(CDK2)

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of various cells using Phospho-DDR1 (Y513) Polyclonal Antibody)

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DDR1, ELISA Kit (Cat# AAA319102)

Full Name

DDR1 (Phospho-Tyr513) Colorimetric Cell-Based ELISA Kit

Gene Names

DDR1; CAK; DDR; NEP; HGK2; PTK3; RTK6; TRKE; CD167; EDDR1; MCK10; NTRK4; PTK3A

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of extracts from Hela cells, 1:2000. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).)

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Histone H2A.X, ELISA Kit (Cat# AAA319106)

Full Name

Histone H2A.X (Phospho-Ser139) Colorimetric Cell-Based ELISA Kit

Gene Names

H2AFX; H2AX; H2A.X; H2A/X

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4 degree C over night)

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IkappaBalpha, ELISA Kit (Cat# AAA319111)

Full Name

IkappaBalpha (Phospho-Ser32) Colorimetric Cell-Based ELISA Kit

Gene Names

NFKBIA; IKBA; MAD-3; NFKBI

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of various cells using Phospho-LIMK-2 (T505) Polyclonal Antibody)

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LIMK-2, ELISA Kit (Cat# AAA319112)

Full Name

LIMK-2 (Phospho-Thr505) Colorimetric Cell-Based ELISA Kit

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of PDGFR alpha (Phospho-Tyr754) Antibody. The lane on the right is blocked with the PDGFR alpha (Phospho-Tyr754) peptide.)

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PDGFR-alpha, ELISA Kit (Cat# AAA319119)

Full Name

PDGFR-alpha (Phospho-Tyr754) Colorimetric Cell-Based ELISA Kit

Gene Names

PDGFRA; CD140A; PDGFR2; PDGFR-2; RHEPDGFRA

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from Jarkat cells, primary antibody was diluted at 1:1000, 4 degree C over night)

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SEK1/MKK4, ELISA Kit (Cat# AAA319123)

Full Name

SEK1/MKK4 (Phospho-Ser80) Colorimetric Cell-Based ELISA Kit

Gene Names

MAP2K4; JNKK; MEK4; MKK4; SEK1; JNKK1; SERK1; MAPKK4; PRKMK4; SAPKK1; SAPKK-1

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from 293 cells treated with PMA 125ng/ml 30', using TR-beta1 (Phospho-Ser142) Antibody. The lane on the right is blocked with the phospho peptide.)

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TRbeta1, ELISA Kit (Cat# AAA319127)

Full Name

TRbeta1 (Phospho-Ser142) Colorimetric Cell-Based ELISA Kit

Gene Names

THRB; GRTH; PRTH; THR1; ERBA2; NR1A2; THRB1; THRB2; C-ERBA-2; C-ERBA-BETA

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of 3T3 cells using Phospho-Tensin-2 (Y483) Polyclonal Antibody diluted at 1:2000)

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Tensin-2, ELISA Kit (Cat# AAA319147)

Full Name

Tensin-2 (Phospho-Tyr483) Colorimetric Cell-Based ELISA Kit

Gene Names

TNS2; C1TEN; TENC1; C1-TEN

Reactivity

Human, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from Jarkat cells, primary antibody was diluted at 1:1000, 4 degree C over night)

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RCC1, ELISA Kit (Cat# AAA319149)

Full Name

RCC1 (Phospho-Ser11) Colorimetric Cell-Based ELISA Kit

Gene Names

RCC1; CHC1; RCC1-I; SNHG3-RCC1

Reactivity

Human, Rat, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4 degree C over night)

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PZR, ELISA Kit (Cat# AAA319154)

Full Name

PZR (Phospho-Tyr263) Colorimetric Cell-Based ELISA Kit

Gene Names

MPZL1; PZR; PZRa; PZRb; PZR1b; MPZL1b

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of extracts from NIH-3T3 cells, using Phospho-Annexin I (Y21) Polyclonal Antibody.)

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Annexin I, ELISA Kit (Cat# AAA319159)

Full Name

Annexin I (Phospho-Tyr21) Colorimetric Cell-Based ELISA Kit

Gene Names

ANXA1; ANX1; LPC1

Reactivity

Human, Rat, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of various cells using Phospho-Cdk2/Cdc2 (T160) Polyclonal Antibody diluted at 1:500)

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Cdk2/Cdc2, ELISA Kit (Cat# AAA319162)

Full Name

Cdk2/Cdc2 (Phospho-Thr160) Colorimetric Cell-Based ELISA Kit

Gene Names

CDK2; p33(CDK2)

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

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WB (Western Blot) (Western blot analysis of lysates from DU145 cells, primary antibody was diluted at 1:1000, 4 degree over night)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung cancer. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).)

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MEK-4, Polyclonal Antibody (Cat# AAA319464)

Full Name

MEK-4 (Phospho-Ser257) Antibody

Gene Names

MAP2K4; JNKK; MEK4; MKK4; SEK1; JNKK1; SERK1; MAPKK4; PRKMK4; SAPKK1; SAPKK-1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$300/0.05 mL $420/0.1 mL $660/0.2 mL $1,285/2x0.2 mL $1,875/3x0.2 mL $2,430/4x0.2 mL $2,735/5x0.2 mL

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WB (Western Blot) (Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4 degree over night)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).)

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MFF, Polyclonal Antibody (Cat# AAA319474)

Full Name

MFF (Phospho-Ser146) Antibody

Gene Names

MFF; EMPF2; GL004; C2orf33

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Pricing

$300/0.05 mL $420/0.1 mL $660/0.2 mL $1,285/2x0.2 mL $1,875/3x0.2 mL $2,430/4x0.2 mL $2,735/5x0.2 mL

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WB (Western Blot) (Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4 degree over night)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).)

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MOB1, Polyclonal Antibody (Cat# AAA319475)

Full Name

MOB1 (Phospho-Thr12) Antibody

Gene Names

MOB1A; MOB1; MATS1; Mob4B; C2orf6; MOBK1B; MOBKL1B

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Pricing

$300/0.05 mL $420/0.1 mL $660/0.2 mL $1,285/2x0.2 mL $1,875/3x0.2 mL $2,430/4x0.2 mL $2,735/5x0.2 mL

WB (Western Blot) (Western Blot analysis of 3T3 cells using Phospho-MerTK/Tyro3 (Y749/681) Polyclonal Antibody diluted at 1:500)

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MerTK/Tyro3, ELISA Kit (Cat# AAA319254)

Full Name

MerTK/Tyro3 (Phospho-Tyr749/681) Colorimetric Cell-Based ELISA Kit

Gene Names

MERTK; MER; RP38; c-mer

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of 293T cells using Antibody diluted at 500. Secondary antibody was diluted at 1:20000)

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MER/TYRO3, ELISA Kit (Cat# AAA319255)

Full Name

MER/TYRO3 (Phospho-Tyr753/Tyr685) Colorimetric Cell-Based ELISA Kit

Gene Names

MERTK; MER; RP38; c-mer

Reactivity

Human: Y753/Y685, Mouse: Y748/Y675, Rat: Y748/Y675

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from HepG2 cells treated with PMA 125ng/ml 30', using CD227/MUC1 (Phospho-Tyr1229) Antibody. The lane on the right is blocked with the phospho peptide.)

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Mucin 1, ELISA Kit (Cat# AAA319259)

Full Name

Mucin 1 (Phospho-Tyr1229) Colorimetric Cell-Based ELISA Kit

Gene Names

MUC1; EMA; PEM; PUM; KL-6; MAM6; PEMT; CD227; H23AG; MUC-1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC

Reactivity

Human, Rat, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of various cells using Phospho-NFkappaB-p105/p50 (S337) Polyclonal Antibody diluted at 1:500)

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NFkappaB-p105/p50, ELISA Kit (Cat# AAA319264)

Full Name

NFkappaB-p105/p50 (Phospho-Ser337) Colorimetric Cell-Based ELISA Kit

Gene Names

NFKB1; p50; KBF1; p105; EBP-1; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B

Reactivity

Human, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of Jurkat cells using Phospho-p47-phox (S345) Polyclonal Antibody diluted at 1:1000)

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p47-phox, ELISA Kit (Cat# AAA319267)

Full Name

p47-phox (Phospho-Ser345) Colorimetric Cell-Based ELISA Kit

Gene Names

NCF1; NCF1A; NOXO2; p47phox; SH3PXD1A

Reactivity

Human, Rat, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from Jurkat cells treated with PMA 125ng/ml 30', using SRF (Phospho-Ser77) Antibody. The lane on the right is blocked with the phospho peptide.)

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SRF, ELISA Kit (Cat# AAA319277)

Full Name

SRF (Phospho-Ser77) Colorimetric Cell-Based ELISA Kit

Gene Names

SRF; MCM1

Reactivity

Human, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from Jurkat cells treated with PMA 125ng/ml 30', using TAL-1 (Phospho-Ser122) Antibody. The lane on the right is blocked with the phospho peptide.)

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TAL1, ELISA Kit (Cat# AAA319279)

Full Name

TAL1 (Phospho-Ser122) Colorimetric Cell-Based ELISA Kit

Gene Names

TAL1; SCL; TCL5; tal-1; bHLHa17

Reactivity

Human, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of JK cells using Phospho-Vav3 (Y173) Polyclonal Antibody)

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Vav3, ELISA Kit (Cat# AAA319282)

Full Name

Vav3 (Phospho-Tyr173) Colorimetric Cell-Based ELISA Kit

Reactivity

Human, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of HepG2 cells using Phospho-XIAP (S87) Polyclonal Antibody diluted at 1:500)

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XIAP, ELISA Kit (Cat# AAA319284)

Full Name

XIAP (Phospho-Ser87) Colorimetric Cell-Based ELISA Kit

Gene Names

XIAP; API3; ILP1; MIHA; XLP2; BIRC4; IAP-3; hIAP3; hIAP-3

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from HepG2 cells treated with PMA 125ng/ml 15', using YB1 (Phospho-Ser102) Antibody. The lane on the right is blocked with the phospho peptide.)

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YB-1, ELISA Kit (Cat# AAA319285)

Full Name

YB-1 (Phospho-Ser102) Colorimetric Cell-Based ELISA Kit

Gene Names

YBX1; YB1; BP-8; CSDB; DBPB; YB-1; CSDA2; NSEP1; NSEP-1; MDR-NF1

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of extracts from K562 cells, using Phospho-EpoR (Y426) Polyclonal Antibody.)

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EpoR, ELISA Kit (Cat# AAA319287)

Full Name

EpoR (Phospho-Tyr426) Colorimetric Cell-Based ELISA Kit

Gene Names

EPOR; EPO-R

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

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WB (Western Blot) (Western blot analysis of Phospho-CDC37 (Ser13) expression in various lysates)

WB (Western Blot) (Western blot analysis of CDC37 phosphorylation expression in NIH-3T3 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

IHC (Immunohiostchemistry) (AAA321746 at 1/200 staining human colon cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IF (Immunofluorescence) (AAA321746 staining NIH-3T3 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

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CDC37, Polyclonal Antibody (Cat# AAA321746)

Full Name

Phospho-CDC37 (Ser13) Antibody

Gene Names

CDC37; P50CDC37

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of Phospho-WNK1 (Thr58) expression in various lysates)

WB (Western Blot) (Western blot analysis of WNK1 phosphorylation expression in EGF treated 293 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

IHC (Immunohiostchemistry) (AAA321771 at 1/100 staining human Lung carcinoma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IF (Immunofluorescence) (AAA321771 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

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WNK1, Polyclonal Antibody (Cat# AAA321771)

Full Name

Phospho-WNK1 (Thr58) Antibody

Gene Names

WNK1; KDP; PSK; p65; HSN2; HSAN2; PRKWNK1; PPP1R167

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IF (Immunofluorescence) (AAA321773 staining HepG2 cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)

IHC (Immunohistochemistry) (AAA321773 at 1/100 staining human testis tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of HDAC6 phosphorylation expression in NIH-3T3 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

WB (Western Blot) (Western blot analysis of Phospho-HDAC6 (Ser22) Antibody expression in NIH-3T3 cells lysates.The lane on the right is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA321773 staining NIH-3T3 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

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HDAC6, Polyclonal Antibody (Cat# AAA321773)

Full Name

Phospho-HDAC6 (Ser22) Antibody

Gene Names

HDAC6; HD6; JM21; CPBHM; PPP1R90

Reactivity

Human, Mouse

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of lysates from HT29 cells treated with heat shock, using Vitamin D Receptor (Phospho-Ser208) Antibody. The lane on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells, using Vitamin D Receptor (Phospho-Ser208) Antibody. The picture on the right is blocked with the phospho peptide.)

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Vitamin D Receptor, Polyclonal Antibody (Cat# AAA316449)

Full Name

Vitamin D Receptor (Phospho-Ser208) Antibody

Gene Names

VDR; NR1I1; PPP1R163

Reactivity

Human

Applications

Immunofluorescence, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

Application Data (The TFact Estrogen Receptor-beta (Phospho-Ser105) DNA-Binding ELISA detects active Estrogen Receptor-beta (Phospho-Ser105) in Hela Nuclear Extract. The Hela cells were grown 3 days in DMEM with 10% FBS and harvested for nuclear extract. The Hela cells were stimulated by UV (100J/M2) or directly harvested without UV stimulation.)

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Estrogen Receptor-beta, ELISA Kit (Cat# AAA315883)

Full Name

Estrogen Receptor-beta (Phospho-Ser105) TFact DNA-Binding ELISA Kit

Gene Names

ESR2; Erb; ESRB; ESTRB; NR3A2; ER-BETA; ESR-BETA

Reactivity

Human, Mouse

Pricing

$635/1 Kit $1,230/2x1 Kit $1,800/3x1 Kit $2,335/4x1 Kit $2,905/5x1 Kit

Application Data (The TFact Elk1 (Phospho-Ser389) DNA-Binding ELISA detects active Elk1 (Phospho-Ser389) in Jurkat Nuclear Extract. The Jurkat cells were grown 3 days in RPMI 1640 with 10% FBS and harvested for nuclear extract. The DNA-transcription factor complex is treated with stablization buffer.)

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Elk1, ELISA Kit (Cat# AAA315888)

Full Name

Elk1 (Phospho-Ser389) TFact DNA-Binding ELISA Kit

Reactivity

Human, Mouse

Pricing

$635/1 Kit $1,230/2x1 Kit $1,800/3x1 Kit $2,335/4x1 Kit $2,905/5x1 Kit

Application Data (The TFact ETS1 (Phospho-Thr38) DNA-Binding ELISA detects active ETS1 (Phospho-Thr38) in Jurkat Nuclear Extract. The Jurkat cells were grown 3 days in RPMI 1640 with 10% FBS and harvested for nuclear extract. Jurkat cells were stimulated by PMA (200nM) before harvest.)

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ETS1, ELISA Kit (Cat# AAA315893)

Full Name

ETS1 (Phospho-Thr38) TFact DNA-Binding ELISA Kit

Gene Names

ETS1; p54; ETS-1; EWSR2; c-ets-1

Reactivity

Human, Mouse

Pricing

$635/1 Kit $1,230/2x1 Kit $1,800/3x1 Kit $2,335/4x1 Kit $2,905/5x1 Kit

Application Data (The TFact FOXO1 (Phospho-Ser319) DNA-Binding ELISA detects active FKHR (Phospho-Ser319) in Hela Nuclear Extract. The Hela cells were grown 3 days in DMEM with 10% FBS and harvested for nuclear extract. The Hela cells were stimulated by serum (20% FBS) before harvest.)

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FOXO1, ELISA Kit (Cat# AAA315902)

Full Name

FOXO1 (Phospho-Ser319) TFact DNA-Binding ELISA Kit

Gene Names

FOXO1; FKH1; FKHR; FOXO1A

Reactivity

Human, Mouse, Rat

Pricing

$635/1 Kit $1,230/2x1 Kit $1,800/3x1 Kit $2,335/4x1 Kit $2,905/5x1 Kit

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WB (Western Blot) (Western blot analysis of lysates from Jurkat cells treated with starved 24h, using Kv1.3/KCNA3 (Phospho-Tyr135) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using Kv1.3/KCNA3 (Phospho-Tyr135) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HUVEC cells, using Kv1.3/KCNA3 (Phospho-Tyr135) Antibody. The picture on the right is blocked with the phospho peptide.)

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Kv1.3/KCNA3, Polyclonal Antibody (Cat# AAA316415)

Full Name

Kv1.3/KCNA3 (Phospho-Tyr135) Antibody

Gene Names

KCNA3; MK3; HGK5; HLK3; PCN3; HPCN3; KV1.3; HUKIII

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from 293 cells treated with EGF 200ng/ml 30', using Breast Tumor Kinase (Phospho-Tyr447) Antibody. The lane on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells, using Breast Tumor Kinase (Phospho-Tyr447) Antibody. The picture on the right is blocked with the phospho peptide.)

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Breast Tumor Kinase, Polyclonal Antibody (Cat# AAA316298)

Full Name

Breast Tumor Kinase (Phospho-Tyr447) Antibody

Gene Names

PTK6; BRK

Reactivity

Human

Applications

Immunofluorescence, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

What Are Phospho Antibodies?

Protein phosphorylation is a process where a phosphate group is added to certain amino acid residues of a protein – usually serine (S), threonine (T), or tyrosine (Y) - by enzymes called kinases. This process is integral in controlling cellular signaling, cellular growth, and other biological functions.

Our catalog includes a wide range of phospho-specific antibodies that can accurately detect this important marker. They perform strongly in widely-used laboratory applications such as Western blot, flow cytometry, immunohistochemistry, and immunofluorescence microscopy. We value your trust in us and are committed to providing top-quality products and services. All of our antibodies are guaranteed to work for the applications and species indicated on our website & associated product pages.

What Are The Key Applications of Phospho Antibodies?

1. Western Blotting

One of the first steps a researcher can take in utilizing these phospho-specific antibodies, is to check if the antibody works using a technique referred to as “Western blot”. For those unfamiliar, Western Blot aids in showing whether the protein that the antibody recognizes is appearing at the correct/expected size. These phospho-specific antibodies should also be able to detect changes in the target protein’s phosphorylation (on/off state) when cells are stimulated in certain ways.

2. Staining of Fixed Cells (Immunocytochemistry)

Another routine use of these phospho-specific antibodies, is to test if the antibody is able to demonstrate similar performance when used on fixed cells (intact cells that have been preserved) as it did in the Western blot tests. It is an important aspect in many cases to confirm that the antibody works in actual intact cell samples. Ideally, the method used for cellular fixation should be the same as what is used in pathology labs (like using 10% formalin). To check if the antibody works well in tissue sections (FFPE), researchers will often test it on fixed cells that are processed similar to tissue samples.

3. Specificity Tests Using Peptides

In order to make sure that the antibody is only binding to the right target:

• Laboratory technicians will mix the antibody with phospho-peptides (short segments of the protein containing the phosphate group modification).


• If the antibody signal disappears, it is confirmation that it is binding to the correct phosphorylated location.


• A more robust test is to use both the phosphorylated and non-phosphorylated (dephosphorylated) versions of the protein. The antibody should react only with the phosphorylated one.


• Another method sometimes utilized is to treat the sample with an enzyme, such as alkaline phosphatase, that specifically removes phosphate groups. If the antibody signal disappears after this, it also confirms specificity.

4. Genetic Confirmation

As a final step, scientists can genetically manipulate the nucleotide sequence and alter the target protein by removing the exact site where phosphorylation happens. If the antibody no longer appears to detect the modified protein, it is strong evidence supporting the antibody being specific for that phosphorylated site.

Why Buy Phospho Antibodies Through Us?

• The production laboratory adheres to strict and consistent protocols prior to releasing any of these phospho-specific antibodies:


• Standard methods and proper controls in all tests to ensure high quality.


• These antibodies are tested and validated in different cell types and species.


• High quality control criterion to ensure each batch is consistent, so you will obtain reliable results every time.

FAQ

1. What Are Phospho-Specific Antibodies?

Phospho-specific antibodies are made to detect proteins only when they have a phosphate group linked to a specific amino acid residue. This empowers scientists understand if a protein is "turned on" or active, based on its phosphorylation state.

2. How to Detect Phosphorylated Proteins in a Western Blot?

To find out if a protein is phosphorylated using Western blot:

• Use a phospho-specific antibody that binds only to the phosphorylated form of the protein.


• You can also use a “regular” antibody for the same amino acid sequence of the protein that the phospho-specific antibody is binding to (but in this case, this antibody will not bind if there is a phosphate group present) in order to compare how much of it is phosphorylated versus how much is non-phosphorylated (or “total” protein, if the “normal” antibody’s epitopes are non-phospho-site-specific).

3. How to Choose the Best Antibody?

Here are some simple tips to help you pick the right antibody:

• Know your target


• Match your sample characteristics


• Confirm the intended use is appropriate


• Check “host” and “type”


• Check the “quality” of the presented data/images


• Appraise whether the available validation meets your needs