Phospho Antibodies

Phospho-specific antibodies’ typical purpose is to enable researchers to detect changes in proteins. They will exclusively bind to the amino acid sequence on a protein that has been phosphorylated (which is both a physical & chemical change) and do not bind to the same amino acid sequence on said protein if it lacks said phosphorylation. This aids in being able to clearly see and understand the data produced from this particular protein modification.

WB (Western Blot) (Western Blot analysis of K562 cells, cell treated or untreated by EGF 20ng/ml 30'. Primary Antibody was diluted at 1:1000. Secondary antibody(was diluted at 1:10000)

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eNOS, ELISA Kit (Cat# AAA319265)

Full Name

eNOS (Phospho-Ser1179) Colorimetric Cell-Based ELISA Kit

Gene Names

NOS3; eNOS; ECNOS

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from LOVO cells treated with PMA 125ng/ml 30', using PAK5/6 (Phospho-Ser602/Ser560) Antibody. The lane on the right is blocked with the phospho peptide.)

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PAK5/6, ELISA Kit (Cat# AAA319269)

Full Name

PAK5/6 (Phospho-Ser602/S560) Colorimetric Cell-Based ELISA Kit

Gene Names

PAK7; PAK5

Reactivity

Human, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from LOVO cells treated with starved 24h, using PAKalpha (Phospho-Ser199) Antibody. The lane on the right is blocked with the phospho peptide.)

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PAKalpha, ELISA Kit (Cat# AAA319270)

Full Name

PAKalpha (Phospho-Ser199) Colorimetric Cell-Based ELISA Kit

Gene Names

PAK1; PAKalpha

Reactivity

Human, Mouse, Rat, Monkey

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of various cells using Phospho-Paxillin (Y88) Polyclonal Antibody)

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Paxillin, ELISA Kit (Cat# AAA319271)

Full Name

Paxillin (Phospho-Tyr88) Colorimetric Cell-Based ELISA Kit

Reactivity

Human, Rat, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of HELA-UV 293T-UV using p-Rad51 (T309) antibody. Antibody was diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).)

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Rad51, ELISA Kit (Cat# AAA319276)

Full Name

Rad51 (Phospho-Thr309) Colorimetric Cell-Based ELISA Kit

Gene Names

RAD51; RECA; BRCC5; MRMV2; HRAD51; RAD51A; HsRad51; HsT16930

Reactivity

Human, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of 3T3 cells using Phospho-STAM2 (Y192) Polyclonal Antibody)

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STAM2, ELISA Kit (Cat# AAA319278)

Full Name

STAM2 (Phospho-Tyr192) Colorimetric Cell-Based ELISA Kit

Gene Names

STAM2; Hbp; STAM2A; STAM2B

Reactivity

Human, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from Jurkat cells, using ZAP-70 (Phospho-Tyr319) Antibody. The lane on the right is blocked with the phospho peptide.)

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ZAP-70, ELISA Kit (Cat# AAA319286)

Full Name

ZAP-70 (Phospho-Tyr319) Colorimetric Cell-Based ELISA Kit

Gene Names

ZAP70; SRK; STD; TZK; STCD; ZAP-70

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

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IF (Immunofluorescence) (AAA323703 staining SK-OV3 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of Phospho-EGF Receptor (Tyr998) antibody using 293 whole cell lysates)

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EGF Receptor, Polyclonal Antibody (Cat# AAA323703)

Full Name

Phospho-EGF Receptor (Tyr998) Antibody

Gene Names

EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2

Reactivity

Human, Mouse

Applications

Immunocytochemistry, Immunofluorescence, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

Application Data (The TFact AML1 (Phospho-Ser303) DNA-Binding ELISA detects active AML1 (Phospho-Ser303) in Hela Nuclear Extract. The Hela cells were grown 3 days in DMEM with 10% FBS and harvested for nuclear extract. The Hela cells were stimulated by PMA (200nM) before harvest.)

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AML1, ELISA Kit (Cat# AAA315845)

Full Name

AML1 (Phospho-Ser303) TFact DNA-Binding ELISA Kit

Gene Names

TADA2A; ADA2; ADA2A; KL04P; hADA2; TADA2L

Reactivity

Human, Mouse, Rat

Pricing

$635/1 Kit $1,230/2x1 Kit $1,800/3x1 Kit $2,335/4x1 Kit $2,905/5x1 Kit

Application Data (The TFact AML1 (Phospho-Ser435) DNA-Binding ELISA detects active AML1 (Phospho-Ser435) in Hela Nuclear Extract. The Hela cells were grown 3 days in DMEM with 10% FBS and harvested for nuclear extract. The Hela cells were stimulated by PMA (200nM) before harvest.)

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AML1, ELISA Kit (Cat# AAA315846)

Full Name

AML1 (Phospho-Ser435) TFact DNA-Binding ELISA Kit

Gene Names

RUNX1; AML1; CBFA2; EVI-1; AMLCR1; PEBP2aB; CBF2alpha; AML1-EVI-1; PEBP2alpha

Reactivity

Human, Mouse

Pricing

$635/1 Kit $1,230/2x1 Kit $1,800/3x1 Kit $2,335/4x1 Kit $2,905/5x1 Kit

Application Data (The TFact c-Jun DNA-Binding ELISA kits detect active c-Jun in Hela Nuclear Extract. The Hela cells were grown 3 days in DMEM with 10% FBS and harvested for nuclear extract. The Hela cells were stimulated by UV (100J/M2) before harvest.)

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c-Jun, ELISA Kit (Cat# AAA315849)

Full Name

c-Jun (Phospho-Ser249) TFact DNA-Binding ELISA Kit

Gene Names

JUN; AP1; p39; AP-1; c-Jun

Reactivity

Human, Mouse, Rat

Pricing

$635/1 Kit $1,230/2x1 Kit $1,800/3x1 Kit $2,335/4x1 Kit $2,905/5x1 Kit

Application Data (The TFact Androgen Receptor (Phospho-Ser650) DNA-Binding ELISA detects active Androgen Receptor (Phospho-Ser650) in MCF7 Nuclear Extract. MCF7 cells were grown 3 days in DMEM with 10% FBS and harvested for nuclear extract.)

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Androgen Receptor, ELISA Kit (Cat# AAA315863)

Full Name

Androgen Receptor (Phospho-Ser650) TFact DNA-Binding ELISA Kit

Gene Names

AR; KD; AIS; AR8; TFM; DHTR; SBMA; HYSP1; NR3C4; SMAX1; HUMARA

Reactivity

Human, Mouse, Rat

Pricing

$635/1 Kit $1,230/2x1 Kit $1,800/3x1 Kit $2,335/4x1 Kit $2,905/5x1 Kit

Application Data (The TFact ATF2 (Phospho-Ser480) DNA-Binding ELISA detects active ATF2 (Phospho-Ser480) in Hela Nuclear Extract. The Hela cells were grown 3 days in DMEM with 10% FBS and harvested for nuclear extract. The Hela cells were stimulated by UV (100J/M2) before harvest. The DNA-transcription factor complex is treated with stablization buffer.)

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ATF2, ELISA Kit (Cat# AAA315867)

Full Name

ATF2 (Phospho-Ser480) TFact DNA-Binding ELISA Kit

Gene Names

ATF2; HB16; CREB2; TREB7; CREB-2; CRE-BP1

Reactivity

Human, Mouse, Rat

Pricing

$635/1 Kit $1,230/2x1 Kit $1,800/3x1 Kit $2,335/4x1 Kit $2,905/5x1 Kit

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WB (Western Blot) (Western blot analysis of lysates from HUVEC cells treated with Serum 20% 30', using Histone H3 (Phospho-Thr3) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using Histone H3 (Phospho-Thr3) Antibody. The picture on the right is blocked with the phospho peptide.)

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Histone H3, Polyclonal Antibody (Cat# AAA316146)

Full Name

Histone H3 (Phospho-Thr3) Antibody

Gene Names

HIST1H3A; H3/A; H3FA

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HepG2 cells treated with EGF 200ng/ml 30', using Artemis (Phospho-Ser516) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using Artemis (Phospho-Ser516) Antibody. The picture on the right is blocked with the phospho peptide.)

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Artemis, Polyclonal Antibody (Cat# AAA316159)

Full Name

Artemis (Phospho-Ser516) Antibody

Gene Names

DCLRE1C; SCIDA; SNM1C; A-SCID; RS-SCID; DCLREC1C

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

Application Data (The TFact CREB DNA-Binding ELISA kits detect active CREB in Hela Nuclear Extract. The Hela cells were grown 3 days in DMEM with 10% FBS and harvested for nuclear extract. The Hela cells were stimulated by UV (100J/M2) before harvest.)

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CREB, ELISA Kit (Cat# AAA315876)

Full Name

CREB (Phospho-Ser133) TFact DNA-Binding ELISA Kit

Gene Names

CREB1; CREB; CREB-1

Reactivity

Human, Mouse, Rat

Pricing

$735/1 Kit $1,435/2x1 Kit $2,095/3x1 Kit $2,720/4x1 Kit $3,355/5x1 Kit

Application Data (The TFact Elk1 (Phospho-Thr417) DNA-Binding ELISA detects active Elk1 (Phospho-Thr417) in Jurkat Nuclear Extract. The Jurkat cells were grown 3 days in RPMI 1640 with 10% FBS and harvested for nuclear extract. The DNA-transcription factor complex is treated with stablization buffer.)

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Elk1, ELISA Kit (Cat# AAA315889)

Full Name

Elk1 (Phospho-Thr417) TFact DNA-Binding ELISA Kit

Reactivity

Human, Mouse

Pricing

$635/1 Kit $1,230/2x1 Kit $1,800/3x1 Kit $2,335/4x1 Kit $2,905/5x1 Kit

Application Data (The TFact Elk3 (Phospho-Ser357) DNA-Binding ELISA detects active Elk3 (Phospho-Ser357) in Jurkat Nuclear Extract. The Jurkat cells were grown 3 days in RPMI 1640 with 10% FBS and harvested for nuclear extract. The DNA-transcription factor complex is treated with stablization buffer.)

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Elk3, ELISA Kit (Cat# AAA315891)

Full Name

Elk3 (Phospho-Ser357) TFact DNA-Binding ELISA Kit

Gene Names

ELK3; ERP; NET; SAP2; SAP-2

Reactivity

Human, Mouse, Rat

Pricing

$635/1 Kit $1,230/2x1 Kit $1,800/3x1 Kit $2,335/4x1 Kit $2,905/5x1 Kit

Application Data (The TFact FOXO1 (Phospho-Ser256) DNA-Binding ELISA detects active FKHR (Phospho-Ser256) in Hela Nuclear Extract. The Hela cells were grown 3 days in DMEM with 10% FBS and harvested for nuclear extract. The Hela cells were stimulated by serum (20% FBS) before harvest.)

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FOXO1, ELISA Kit (Cat# AAA315901)

Full Name

FOXO1 (Phospho-Ser256) TFact DNA-Binding ELISA Kit

Gene Names

FOXO1; FKH1; FKHR; FOXO1A

Reactivity

Human, Mouse

Pricing

$635/1 Kit $1,230/2x1 Kit $1,800/3x1 Kit $2,335/4x1 Kit $2,905/5x1 Kit

Application Data (The TFact FKHRL1 (Phospho-Ser253) DNA-Binding ELISA detects active FKHRL1 (Phospho-Ser253) in Hela Nuclear Extract. The Hela cells were grown 3 days in DMEM with 10% FBS and harvested for nuclear extract. The Hela cells were Serum stimulated (20% FBS) before harvest.)

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FKHRL1, ELISA Kit (Cat# AAA315911)

Full Name

FKHRL1 (Phospho-Ser253) TFact DNA-Binding ELISA Kit

Gene Names

FOXO3; FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2

Reactivity

Human, Mouse

Pricing

$635/1 Kit $1,230/2x1 Kit $1,800/3x1 Kit $2,335/4x1 Kit $2,905/5x1 Kit

Application Data (The TFact GATA4 (Phospho-Ser262) DNA-Binding ELISA detects active GATA4 (Phospho-Ser262) in Jurkat Nuclear Extract. The Jurkat cells were grown 3 days in RPMI 1640 with 10% FBS and harvested for nuclear extract. The DNA-transcription factor complex is treated with stablization buffer.)

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GATA4, ELISA Kit (Cat# AAA315916)

Full Name

GATA4 (Phospho-Ser262) TFact DNA-Binding ELISA Kit

Gene Names

GATA4; TOF; ASD2; VSD1; TACHD

Reactivity

Human, Mouse

Pricing

$635/1 Kit $1,230/2x1 Kit $1,800/3x1 Kit $2,335/4x1 Kit $2,905/5x1 Kit

Application Data (The TFact GR (Phospho-Ser211) DNA-Binding ELISA detects active GR (Phospho-Ser211) in MCF7 and Hela Nuclear Extract. The MCF7 and Hela cells were grown 3 days in DMEM and DMEM with 10% FBS and harvested for nuclear extract. The Hela cells were stimulated by UV (100J/M2) before harvest.)

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GR, ELISA Kit (Cat# AAA315920)

Full Name

GR (Phospho-Ser211) TFact DNA-Binding ELISA Kit

Gene Names

NR3C1; GR; GCR; GRL; GCCR; GCRST

Reactivity

Human, Mouse, Rat

Pricing

$635/1 Kit $1,230/2x1 Kit $1,800/3x1 Kit $2,335/4x1 Kit $2,905/5x1 Kit

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WB (Western Blot) (Western blot analysis of lysates from 293 cells treated with PMA 125ng/ml 30', using C-RAF (Phospho-Ser296) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using C-RAF (Phospho-Ser296) Antibody. The picture on the right is blocked with the phospho peptide.)

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C-RAF, Polyclonal Antibody (Cat# AAA316252)

Full Name

C-RAF (Phospho-Ser296) Antibody

Gene Names

RAF1; NS5; CRAF; Raf-1; c-Raf; CMD1NN

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

Application Data (The TFact Smad2 (Phospho-Ser250) DNA-Binding ELISA detects active Smad2 (Phospho-Ser250) in Jurkat Nuclear Extract. The Jurkat cells were grown 3 days in RPMI 1640 with 10% FBS and harvested for nuclear extract.)

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Smad2, ELISA Kit (Cat# AAA315978)

Full Name

Smad2 (Phospho-Ser250) TFact DNA-Binding ELISA Kit

Gene Names

SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2

Reactivity

Human, Mouse, Rat

Pricing

$635/1 Kit $1,230/2x1 Kit $1,800/3x1 Kit $2,335/4x1 Kit $2,905/5x1 Kit

Application Data (The TFact MEF2C (Phospho-Ser396) DNA-Binding ELISA detects active MEF2C (Phospho-Ser396) in Hela Nuclear Extract. The Hela cells were grown 3 days in DMEM with 10% FBS and harvested for nuclear extract. The Hela cells were stimulated by serum (20% FBS) before harvest.)

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MEF2C, ELISA Kit (Cat# AAA315986)

Full Name

MEF2C (Phospho-Ser396) TFact DNA-Binding ELISA Kit

Gene Names

MEF2C; DEL5q14.3; C5DELq14.3

Reactivity

Human, Mouse

Pricing

$635/1 Kit $1,230/2x1 Kit $1,800/3x1 Kit $2,335/4x1 Kit $2,905/5x1 Kit

Application Data (The TFact MEF2D (Phospho-Ser444) DNA-Binding ELISA detects active MEF2D (Phospho-Ser444) in Hela Nuclear Extract. The Hela cells were grown 3 days in DMEM with 10% FBS and harvested for nuclear extract. The Hela cells were stimulated by serum (20% FBS) before harvest.)

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MEF2D, ELISA Kit (Cat# AAA315988)

Full Name

MEF2D (Phospho-Ser444) TFact DNA-Binding ELISA Kit

Reactivity

Human, Mouse, Rat

Pricing

$635/1 Kit $1,230/2x1 Kit $1,800/3x1 Kit $2,335/4x1 Kit $2,905/5x1 Kit

Application Data (The TFact STAT1 (Phospho-Ser727) DNA-Binding ELISA detects active STAT1 (Phospho-Ser727) in Hela and HepG2 Nuclear Extract. The Hela and HepG2 cells were grown 3 days in DMEM with 10% FBS and harvested for nuclear extract. The Hela cells were stimulated by PMA (200nM) before harvest.)

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STAT1, ELISA Kit (Cat# AAA315990)

Full Name

STAT1 (Phospho-Ser727) TFact DNA-Binding ELISA Kit

Gene Names

STAT1; CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT91

Reactivity

Human, Mouse, Rat

Pricing

$635/1 Kit $1,230/2x1 Kit $1,800/3x1 Kit $2,335/4x1 Kit $2,905/5x1 Kit

Application Data (The TFact STAT5A (Phospho-Ser780) DNA-Binding ELISA detects active STAT5A (Phospho-Ser780) in Hela and HepG2 Nuclear Extract. The Hela and HepG2 cells were grown 3 days in DMEM with 10% FBS and harvested for nuclear extract. The Hela cells were stimulated by PMA (200nM) before harvest.)

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STAT5A, ELISA Kit (Cat# AAA315992)

Full Name

STAT5A (Phospho-Ser780) TFact DNA-Binding ELISA Kit

Gene Names

STAT5A; MGF; STAT5

Reactivity

Human, Mouse, Rat

Pricing

$635/1 Kit $1,230/2x1 Kit $1,800/3x1 Kit $2,335/4x1 Kit $2,905/5x1 Kit

Application Data (The TFact Estrogen Receptor-alpha (Phospho-Ser118) DNA-Binding ELISA kit detects active Estrogen Receptor-alpha (Phospho-Ser118) in MCF7 Nuclear Extract. The MCF7 cells were grown 3 days in DMEM with 10% FBS and harvested for nuclear extract.)

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Estrogen Receptor-alpha, ELISA Kit (Cat# AAA316007)

Full Name

Estrogen Receptor-alpha (Phospho-Ser118) TFact DNA-Binding ELISA Kit

Gene Names

ESR1; ER; ESR; Era; ESRA; ESTRR; NR3A1

Reactivity

Human, Mouse

Pricing

$635/1 Kit $1,230/2x1 Kit $1,800/3x1 Kit $2,335/4x1 Kit $2,905/5x1 Kit

Application Data (The TFact STAT1 (Phospho-Tyr701) DNA-Binding ELISA detects active STAT1 (Phospho-Tyr701) in HepG2 Nuclear Extract. The HepG2 cells were grown 3 days in DMEM with 10% FBS and harvested for nuclear extract.)

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STAT1, ELISA Kit (Cat# AAA316015)

Full Name

STAT1 (Phospho-Tyr701) TFact DNA-Binding ELISA Kit

Gene Names

STAT1; CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT91

Reactivity

Human, Mouse, Rat

Pricing

$635/1 Kit $1,230/2x1 Kit $1,800/3x1 Kit $2,335/4x1 Kit $2,905/5x1 Kit

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IF (Immunofluorescence) (Immunofluorescent analysis of HepG2 cells, using Phospho-Nrf2 (S40) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-Nrf2 (S40) Antibody.)

WB (Western Blot) (Western blot analysis of Nrf2 phosphorylation expression in HepG2 cell lysate.)

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Nrf2, Monoclonal Recombinant Antibody (Cat# AAA314877)

Full Name

Nrf2 (Phospho-Ser40) Rabbit mAb

Gene Names

NFE2L2; NRF2

Reactivity

Human

Applications

Flow Cytometry, Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Affinity-chromatography

Pricing

$290/0.05 mL $365/0.1 mL $705/2x0.1 mL $1,030/3x0.1 mL $1,335/4x0.1 mL $1,425/5x0.1 mL

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IF (Immunofluorescence) (AAA321401 staining HepG2 cells (30min of 4uM Forskolin treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA321401) and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).)

IHC (Immunohistochemistry) (AAA321401 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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TAK1, Polyclonal Antibody (Cat# AAA321401)

Full Name

Phospho-TAK1 (Ser439) Antibody

Gene Names

MAP3K7; CSCF; FMD2; TAK1; MEKK7; TGF1a

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Peptide affinity purification.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of ATPase phosphorylation expression in PMA treated 293 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA321472 staining 293 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

IHC (Immunohiostchemistry) (AAA321472 at 1/100 staining Mouse intestine tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of extracts from rat brain/mouse liver, using Phospho-Sodium Potassium ATPase alpha 1 (Ser16) Antibody. -/+ means absence or presence of N peptide(non-phospho peptide) and P peptide(phospho peptide))

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ATPase, Polyclonal Antibody (Cat# AAA321472)

Full Name

Phospho-ATPase (Ser16) Antibody

Gene Names

ATP1A1; CMT2DD

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Peptide affinity purification

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IF (Immunofluorescence) (AAA321564 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

IHC (Immunohiostchemistry) (AAA321564 at 1/200 staining human lymph nodes tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of NF kappaB p105/p50 phosphorylation expression in TNF-alpha treated HeLa whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

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NF kappaB p105/p50, Polyclonal Antibody (Cat# AAA321564)

Full Name

Phospho-NF kappaB p105/p50 (Ser907) Antibody

Gene Names

NFKB1; p50; KBF1; p105; EBP-1; CVID12; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B1

Reactivity

Human

Applications

Immunoprecipitation, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IF (Immunofluorescence) (AAA321578 staining COS7 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of PI3-kinase p85-alpha/gamma phosphorylation expression in H2O2 treated COS7 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

IHC (Immunohistochemistry) (AAA321578 at 1/100 staining Rat colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

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PI3-kinase p85 alpha/gamma, Polyclonal Antibody (Cat# AAA321578)

Full Name

Phospho-PI3-kinase p85 alpha/gamma (Tyr467/199) Antibody

Gene Names

PIK3R1; p85; AGM7; GRB1; IMD36; p85-ALPHA

Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunocytochemistry, Immunofluorescence, Western Blot

Purity

Peptide affinity purification

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohistochemistry) (AAA321581 at 1/100 staining human Lymphoma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of Phospho-Shc (Tyr349) expression in various lysates)

WB (Western Blot) (Western blot analysis of Shc phosphorylation expression in EGF treated 293 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA321581 staining 293 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

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Shc, Polyclonal Antibody (Cat# AAA321581)

Full Name

Phospho-Shc (Tyr349) Antibody

Gene Names

SHC1; SHC; SHCA

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IF (Immunofluorescence) (AAA321651 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of Ataxin 1 phosphorylation expression in Adriamycin treated HepG2 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA321651 staining A549 cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)

WB (Western Blot) (Western blot analysis of Phospho-Ataxin 1 (Ser775) expression in various lysates)

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Ataxin 1, Polyclonal Antibody (Cat# AAA321651)

Full Name

Phospho-Ataxin 1 (Ser775) Antibody

Gene Names

ATXN1; ATX1; SCA1; D6S504E

Reactivity

Human, Mouse

Applications

Immunocytochemistry, Immunofluorescence, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

WB (Western Blot) (Western blot analysis of extracts from COS-7 cells, using Phospho-RhoA (Ser188) Ab. The lane on the left was treated with blocking peptide.)

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RhoA, Polyclonal Antibody (Cat# AAA321656)

Full Name

Phospho-RhoA (Ser188) Antibody

Gene Names

RHOA; ARHA; ARH12; RHO12; RHOH12

Reactivity

Human, Mouse, Rat, Monkey

Applications

Western Blot

Purity

The Ab is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of extracts of MCF-7 cells treated with doxorubicin, using Phospho-p53 (Thr18) Antibody.)

IHC (Immunohistochemistry) (AAA321361 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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p53, Polyclonal Antibody (Cat# AAA321361)

Full Name

Phospho-p53 (Thr18) Antibody

Gene Names

TP53; P53; BCC7; LFS1; TRP53

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of extracts of ZR-75-1 cells, using Phospho-SSH3 (Ser37) Antibody.)

IHC (Immunohistochemistry) (AAA321397 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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SSH3, Polyclonal Antibody (Cat# AAA321397)

Full Name

Phospho-SSH3 (Ser37) Antibody

Gene Names

SSH3; SSH3L

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohiostchemistry) (AAA321319 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts from Hela cells, usingPhospho-Dab1 (Tyr220) Antibody. Lane1 was treated withphospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)

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Dab1, Polyclonal Antibody (Cat# AAA321319)

Full Name

Phospho-Dab1 (Tyr220) Antibody

Gene Names

DAB1; SCA37

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Peptide affinity purification

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of Phospho-Syntaxin 1A (Ser14) expression in various lysates)

IF (Immunofluorescence) (AAA321751 staining NIH/3T3 cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)

WB (Western Blot) (Western blot analysis of Syntaxin 1A phosphorylation expression in Mouse brain tissue lysates, The lane on the left is treated with the antigen-specific peptide.)

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Syntaxin 1A, Polyclonal Antibody (Cat# AAA321751)

Full Name

Phospho-Syntaxin 1A (Ser14) Antibody

Gene Names

STX1A; STX1; HPC-1; P35-1; SYN1A

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of Phospho-HDAC2 (Ser394) expression in various lysates)

WB (Western Blot) (Western blot analysis of extracts from rat spleen, using Phospho-HDAC2 (Ser394) Antibody.)

WB (Western Blot) (Western blot analysis of HDAC2 phosphorylation expression in NIH-3T3 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA321758 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

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HDAC2, Polyclonal Antibody (Cat# AAA321758)

Full Name

Phospho-HDAC2 (Ser394) Antibody

Gene Names

HDAC2; HD2; RPD3; YAF1

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

What Are Phospho Antibodies?

Protein phosphorylation is a process where a phosphate group is added to certain amino acid residues of a protein – usually serine (S), threonine (T), or tyrosine (Y) - by enzymes called kinases. This process is integral in controlling cellular signaling, cellular growth, and other biological functions.

Our catalog includes a wide range of phospho-specific antibodies that can accurately detect this important marker. They perform strongly in widely-used laboratory applications such as Western blot, flow cytometry, immunohistochemistry, and immunofluorescence microscopy. We value your trust in us and are committed to providing top-quality products and services. All of our antibodies are guaranteed to work for the applications and species indicated on our website & associated product pages.

What Are The Key Applications of Phospho Antibodies?

1. Western Blotting

One of the first steps a researcher can take in utilizing these phospho-specific antibodies, is to check if the antibody works using a technique referred to as “Western blot”. For those unfamiliar, Western Blot aids in showing whether the protein that the antibody recognizes is appearing at the correct/expected size. These phospho-specific antibodies should also be able to detect changes in the target protein’s phosphorylation (on/off state) when cells are stimulated in certain ways.

2. Staining of Fixed Cells (Immunocytochemistry)

Another routine use of these phospho-specific antibodies, is to test if the antibody is able to demonstrate similar performance when used on fixed cells (intact cells that have been preserved) as it did in the Western blot tests. It is an important aspect in many cases to confirm that the antibody works in actual intact cell samples. Ideally, the method used for cellular fixation should be the same as what is used in pathology labs (like using 10% formalin). To check if the antibody works well in tissue sections (FFPE), researchers will often test it on fixed cells that are processed similar to tissue samples.

3. Specificity Tests Using Peptides

In order to make sure that the antibody is only binding to the right target:

• Laboratory technicians will mix the antibody with phospho-peptides (short segments of the protein containing the phosphate group modification).


• If the antibody signal disappears, it is confirmation that it is binding to the correct phosphorylated location.


• A more robust test is to use both the phosphorylated and non-phosphorylated (dephosphorylated) versions of the protein. The antibody should react only with the phosphorylated one.


• Another method sometimes utilized is to treat the sample with an enzyme, such as alkaline phosphatase, that specifically removes phosphate groups. If the antibody signal disappears after this, it also confirms specificity.

4. Genetic Confirmation

As a final step, scientists can genetically manipulate the nucleotide sequence and alter the target protein by removing the exact site where phosphorylation happens. If the antibody no longer appears to detect the modified protein, it is strong evidence supporting the antibody being specific for that phosphorylated site.

Why Buy Phospho Antibodies Through Us?

• The production laboratory adheres to strict and consistent protocols prior to releasing any of these phospho-specific antibodies:


• Standard methods and proper controls in all tests to ensure high quality.


• These antibodies are tested and validated in different cell types and species.


• High quality control criterion to ensure each batch is consistent, so you will obtain reliable results every time.

FAQ

1. What Are Phospho-Specific Antibodies?

Phospho-specific antibodies are made to detect proteins only when they have a phosphate group linked to a specific amino acid residue. This empowers scientists understand if a protein is "turned on" or active, based on its phosphorylation state.

2. How to Detect Phosphorylated Proteins in a Western Blot?

To find out if a protein is phosphorylated using Western blot:

• Use a phospho-specific antibody that binds only to the phosphorylated form of the protein.


• You can also use a “regular” antibody for the same amino acid sequence of the protein that the phospho-specific antibody is binding to (but in this case, this antibody will not bind if there is a phosphate group present) in order to compare how much of it is phosphorylated versus how much is non-phosphorylated (or “total” protein, if the “normal” antibody’s epitopes are non-phospho-site-specific).

3. How to Choose the Best Antibody?

Here are some simple tips to help you pick the right antibody:

• Know your target


• Match your sample characteristics


• Confirm the intended use is appropriate


• Check “host” and “type”


• Check the “quality” of the presented data/images


• Appraise whether the available validation meets your needs