Phospho Antibodies

Phospho-specific antibodies’ typical purpose is to enable researchers to detect changes in proteins. They will exclusively bind to the amino acid sequence on a protein that has been phosphorylated (which is both a physical & chemical change) and do not bind to the same amino acid sequence on said protein if it lacks said phosphorylation. This aids in being able to clearly see and understand the data produced from this particular protein modification.

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WB (Western Blot) (Western blot analysis of lysates from MDA-MB-435 cells, using NF-kappaB p105/p50 (Phospho-Ser337) Antibody. The lane on the left is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using NF-kappaB p105/p50 (Phospho-Ser337) Antibody. The picture on the right is blocked with the phospho peptide.)

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NF-kappaB p105/p50, Polyclonal Antibody (Cat# AAA316544)

Full Name

NF-kappaB p105/p50 (Phospho-Ser337) Antibody

Gene Names

NFKB1; p50; KBF1; p105; EBP-1; CVID12; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HeLa cells, using NF-kappaB p105/p50 (Phospho-Ser893) Antibody. The lane on the left is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using NF-kappaB p105/p50 (Phospho-Ser893) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

NF-kappaB p105/p50, Polyclonal Antibody (Cat# AAA316545)

Full Name

NF-kappaB p105/p50 (Phospho-Ser893) Antibody

Gene Names

NFKB1; p50; KBF1; p105; EBP-1; CVID12; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from Jurkat cells, using NF-kappaB p65 (Phospho-Ser276) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using NF-kappaB p65 (Phospho-Ser276) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

NF-kappaB p65, Polyclonal Antibody (Cat# AAA316547)

Full Name

NF-kappaB p65 (Phospho-Ser276) Antibody

Gene Names

RELA; p65; NFKB3

Reactivity

Human, Mouse, Rat

Applications

Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from 293 cells treated with TNF-alpha, using NF-kappaB p65 (Phospho-Thr254) Antibody. The lane on the left is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using NF-kappaB p65 (Phospho-Thr254) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

NF-kappaB p65, Polyclonal Antibody (Cat# AAA316550)

Full Name

NF-kappaB p65 (Phospho-Thr254) Antibody

Gene Names

RELA; p65; NFKB3

Reactivity

Human, Mouse, Rat

Applications

Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from K562 cells treated with Pervanadate, using p73 (Phospho-Tyr99) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using p73 (Phospho-Tyr99) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

p73, Polyclonal Antibody (Cat# AAA316561)

Full Name

p73 (Phospho-Tyr99) Antibody

Gene Names

TP73; P73

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from COS7 cells and A549 cells, using PKC delta (Phospho-Ser645) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PKC delta (Phospho-Ser645) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

PKC delta, Polyclonal Antibody (Cat# AAA316563)

Full Name

PKC delta (Phospho-Ser645) Antibody

Gene Names

PRKCD; MAY1; PKCD; ALPS3; CVID9; nPKC-delta

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from Jurkat cells treated with PMA 200nM 30', using PKC thet (Phospho-Ser676) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PKC thet (Phospho-Ser676) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

PKC thet, Polyclonal Antibody (Cat# AAA316564)

Full Name

PKC thet (Phospho-Ser676) Antibody

Gene Names

PRKCQ; PRKCT; nPKC-theta

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from COS7 cells treated with EGF, using Progesterone Receptor (Phospho-Ser190) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Progesterone Receptor (Phospho-Ser190) Antibody.)

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Progesterone Receptor, Polyclonal Antibody (Cat# AAA316565)

Full Name

Progesterone Receptor (Phospho-Ser190) Antibody

Gene Names

PGR; PR; NR3C3

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HUVEC cells treated with EGF 200ng/ml 5'/HeLa cells treated with EGF 200ng/ml 15', using SMC1 (Phospho-Ser957) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using SMC1 (Phospho-Ser957) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

SMC1, Polyclonal Antibody (Cat# AAA316574)

Full Name

SMC1 (Phospho-Ser957) Antibody

Gene Names

SMC1A; SMC1; SMCB; CDLS2; SB1.8; SMC1L1; DXS423E; SMC1alpha

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from Jurkat cells, using PKC thet (Phospho-Thr538) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using PKC thet (Phospho-Thr538) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

PKC thet, Polyclonal Antibody (Cat# AAA316278)

Full Name

PKC thet (Phospho-Thr538) Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

WB (Western Blot) (Western blot analysis of lysates from 293 cells treated with UV 15', using SGK (Phospho-Ser78) Antibody. The lane on the right is blocked with the phospho peptide.)

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SGK, Polyclonal Antibody (Cat# AAA316282)

Full Name

SGK (Phospho-Ser78) Antibody

Gene Names

SGK1; SGK

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from COS7 cells treated with insulin 0.01U/ML 15', using 53BP1 (Phospho-Ser6) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human heart, using 53BP1 (Phospho-Ser6) Antibody. The picture on the right is blocked with the phospho peptide.)

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53BP1, Polyclonal Antibody (Cat# AAA316286)

Full Name

53BP1 (Phospho-Ser6) Antibody

Gene Names

TP53BP1; TP53; p202; 53BP1; TDRD30; p53BP1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HepG2 cells, using AhR (Phospho-Ser36) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human thyroid gland, using AhR (Phospho-Ser36) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

AhR, Polyclonal Antibody (Cat# AAA316289)

Full Name

AhR (Phospho-Ser36) Antibody

Gene Names

AHR; bHLHe76

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from 293 cells treated with UV 30', using BCL-XL (Phospho-Thr47) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using BCL-XL (Phospho-Thr47) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells, using BCL-XL (Phospho-Thr47) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

BCL-XL, Polyclonal Antibody (Cat# AAA316294)

Full Name

BCL-XL (Phospho-Thr47) Antibody

Gene Names

BCL2L1; BCLX; BCL2L; Bcl-X; PPP1R52; BCL-XL/S

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from COS7 cells, using Bcr (Phospho-Tyr360) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using Bcr (Phospho-Tyr360) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells, using Bcr (Phospho-Tyr360) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

Bcr, Polyclonal Antibody (Cat# AAA316295)

Full Name

Bcr (Phospho-Tyr360) Antibody

Gene Names

BCR; ALL; CML; PHL; BCR1; D22S11; D22S662

Reactivity

Human, Mouse

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HeLa cells treated with Serum 20% 15', using ETK (Phospho-Tyr566) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human thyroid gland, using ETK (Phospho-Tyr566) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

ETK, Polyclonal Antibody (Cat# AAA316300)

Full Name

ETK (Phospho-Tyr566) Antibody

Gene Names

BMX; ETK; PSCTK2; PSCTK3

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from NIH/3T3 cells treated with PMA 250ng/ml 15', using PKC-pan (Phospho-Thr497) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PKC-pan (Phospho-Thr497) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HepG2 cells, using PKC-pan (Phospho-Thr497) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

PKC-pan, Polyclonal Antibody (Cat# AAA316303)

Full Name

PKC-pan (Phospho-Thr497) Antibody

Gene Names

PRKCA; AAG6; PKCA; PRKACA; PKC-alpha

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from COS7 cells treated with UV 30', using PTP1B (Phospho-Ser50) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using PTP1B (Phospho-Ser50) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

PTP1B, Polyclonal Antibody (Cat# AAA316308)

Full Name

PTP1B (Phospho-Ser50) Antibody

Gene Names

PTPN1; PTP1B

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from mouse liver, using BAD (Phospho-Ser134) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using BAD (Phospho-Ser134) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells, using BAD (Phospho-Ser134) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

BAD, Polyclonal Antibody (Cat# AAA316315)

Full Name

BAD (Phospho-Ser134) Antibody

Gene Names

BAD; BBC2; BCL2L8

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from K562 cells treated with insulin 0.01U/ml 15' and Jurkat cells treated with insulin 0.01U/ml 15', using CaMK1-alpha (Phospho-Thr177) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using CaMK1-alpha (Phospho-Thr177) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells, using CaMK1-alpha (Phospho-Thr177) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

CaMK1-alpha, Polyclonal Antibody (Cat# AAA316320)

Full Name

CaMK1-alpha (Phospho-Thr177) Antibody

Gene Names

CAMK1; CAMKI

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from K562 cells, using Caspase 9 (Phospho-Ser144) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using Caspase 9 (Phospho-Ser144) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

Caspase 9, Polyclonal Antibody (Cat# AAA316323)

Full Name

Caspase 9 (Phospho-Ser144) Antibody

Gene Names

CASP9; MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from COS7 cells, using CD226/DNAM-1 (Phospho-Ser329) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using CD226/DNAM-1 (Phospho-Ser329) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells, using CD226/DNAM-1 (Phospho-Ser329) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

CD226/DNAM-1, Polyclonal Antibody (Cat# AAA316327)

Full Name

CD226/DNAM-1 (Phospho-Ser329) Antibody

Gene Names

CD226; PTA1; DNAM1; DNAM-1; TLiSA1

Reactivity

Human, Mouse

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using CD4 (Phospho-Ser433) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HepG2 cells, using CD4 (Phospho-Ser433) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

CD4, Polyclonal Antibody (Cat# AAA316328)

Full Name

CD4 (Phospho-Ser433) Antibody

Gene Names

CD4; CD4mut

Reactivity

Human, Mouse

Applications

Immunofluorescence, Immunohistochemistry

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from 293 cells treated with PMA 125ng/ml 30', using CD5 (Phospho-Tyr453) Antibody. The lane on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HepG2 cells, using CD5 (Phospho-Tyr453) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

CD5, Polyclonal Antibody (Cat# AAA316330)

Full Name

CD5 (Phospho-Tyr453) Antibody

Gene Names

CD5; T1; LEU1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from 293 cells treated with PMA 125ng/ml 30', using CD71/TfR (Phospho-Ser24) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using CD71/TfR (Phospho-Ser24) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

CD71/TfR, Polyclonal Antibody (Cat# AAA316331)

Full Name

CD71/TfR (Phospho-Ser24) Antibody

Gene Names

TFRC; T9; TR; TFR; p90; CD71; TFR1; TRFR; IMD46

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from K562 cells treated with Na3VO4 0.3uM 40', using Chk1 (Phospho-Ser286) Antibody. The lane on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells, using Chk1 (Phospho-Ser286) Antibody. The picture on the right is blocked with the phospho peptide.)

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Chk1, Polyclonal Antibody (Cat# AAA316334)

Full Name

Chk1 (Phospho-Ser286) Antibody

Gene Names

CHEK1; CHK1

Reactivity

Human

Applications

Immunofluorescence, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from 293 cells, using Chk1 (Phospho-Ser301) Antibody. The lane on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells, using Chk1 (Phospho-Ser301) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

Chk1, Polyclonal Antibody (Cat# AAA316335)

Full Name

Chk1 (Phospho-Ser301) Antibody

Gene Names

CHEK1; CHK1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human testis, using c-Jun (Phospho-Thr231) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells, using c-Jun (Phospho-Thr231) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

c-Jun, Polyclonal Antibody (Cat# AAA316337)

Full Name

c-Jun (Phospho-Thr231) Antibody

Gene Names

JUN; AP1; p39; AP-1; c-Jun

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from RAW264.7 cells treated with TNF 20ng/ml 30', using Coagulation Factor III (Phospho-Ser290) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using Coagulation Factor III (Phospho-Ser290) Antibody. The picture on the right is blocked with the phospho peptide.)

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Coagulation Factor III, Polyclonal Antibody (Cat# AAA316340)

Full Name

Coagulation Factor III (Phospho-Ser290) Antibody

Gene Names

F3; TF; TFA; CD142

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from HUVEC cells, using Catenin-delta1 (Phospho-Tyr228) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using Catenin-delta1 (Phospho-Tyr228) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HUVEC cells, using Catenin-delta1 (Phospho-Tyr228) Antibody. The picture on the right is blocked with the phospho peptide.)

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Catenin-delta1, Polyclonal Antibody (Cat# AAA316346)

Full Name

Catenin-delta1 (Phospho-Tyr228) Antibody

Gene Names

CTNND1; CAS; p120; BCDS2; CTNND; P120CAS; P120CTN; p120(CAS); p120(CTN)

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from COS7 cells treated with anisomycin 25ug/ml 30', using CRYAB (Phospho-Ser45) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human heart, using CRYAB (Phospho-Ser45) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

CRYAB, Polyclonal Antibody (Cat# AAA316348)

Full Name

CRYAB (Phospho-Ser45) Antibody

Gene Names

CRYAB; MFM2; CRYA2; CTPP2; HSPB5; CMD1II; CTRCT16; HEL-S-101

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human skeletal muscle, using DAPK2 (Phospho-Ser318) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of COS7 cells, using DAPK2 (Phospho-Ser318) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

DAPK2, Polyclonal Antibody (Cat# AAA316349)

Full Name

DAPK2 (Phospho-Ser318) Antibody

Gene Names

DAPK2; DRP1; DRP-1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from 293 cells treated with Insulin 0.01U/ml 2', using DAPP1 (Phospho-Tyr139) Antibody. The lane on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells, using DAPP1 (Phospho-Tyr139) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

DAPP1, Polyclonal Antibody (Cat# AAA316351)

Full Name

DAPP1 (Phospho-Tyr139) Antibody

Gene Names

DAPP1; BAM32

Reactivity

Human, Mouse

Applications

Immunofluorescence, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using DDX5/DEAD-box Protein 5 (Phospho-Tyr593) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells, using DDX5/DEAD-box Protein 5 (Phospho-Tyr593) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

DDX5/DEAD-box Protein 5, Polyclonal Antibody (Cat# AAA316353)

Full Name

DDX5/DEAD-box Protein 5 (Phospho-Tyr593) Antibody

Gene Names

DDX5; p68; HLR1; G17P1; HUMP68

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using DNA-PK (Phospho-Thr2647) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HUVEC cells treated with serum 20% 30', using DNA-PK (Phospho-Thr2647) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

DNA-PK, Polyclonal Antibody (Cat# AAA316355)

Full Name

DNA-PK (Phospho-Thr2647) Antibody

Gene Names

PRKDC; HYRC; p350; DNAPK; DNPK1; HYRC1; IMD26; XRCC7; DNA-PKcs

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from HepG2 cells, using EPHA2/3 (Phospho-Tyr588/596) Antibody. The lane on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells, using EPHA2/3 (Phospho-Tyr588/596) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

EPHA2/3, Polyclonal Antibody (Cat# AAA316356)

Full Name

EPHA2/3 (Phospho-Tyr588/596) Antibody

Gene Names

EPHA2; ECK; CTPA; ARCC2; CTPP1; CTRCT6

Reactivity

Human, Rat

Applications

Immunofluorescence, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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<< Previous Next >>

WB (Western Blot) (Western Blot analysis of 1 HeLa cell, 2 Serum-free treated ,using primary antibody at 1:1000 dilution. Secondary antibody)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).)

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MYPT1/2, Polyclonal Antibody (Cat# AAA319926)

Full Name

MYPT1/2 (Phospho-Ser668/Ser618) Antibody

Gene Names

PPP1R12A; MBS; M130; MYPT1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Pricing

$385/0.05 mL $625/0.1 mL $965/0.2 mL $1,880/2x0.2 mL $2,745/3x0.2 mL $3,565/4x0.2 mL $4,115/5x0.2 mL

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<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from NIH/3T3 cells, using MYPT1 (Phospho-Thr853) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using MYPT1 (Phospho-Thr853) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

MYPT1, Polyclonal Antibody (Cat# AAA316196)

Full Name

MYPT1 (Phospho-Thr853) Antibody

Gene Names

PPP1R12A; MBS; M130; MYPT1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from HeLa cells treated with Ca+ 40nM 30', using NFAT4 (Phospho-Ser165) Antibody. The lane on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells, using NFAT4 (Phospho-Ser165) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

NFAT4, Polyclonal Antibody (Cat# AAA316197)

Full Name

NFAT4 (Phospho-Ser165) Antibody

Gene Names

NFATC3; NFAT4; NFATX

Reactivity

Human, Mouse

Applications

Immunofluorescence, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from COLO205 cells treated with serum 20% 15', using p70 S6 Kinase (Phospho-Ser371) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using p70 S6 Kinase (Phospho-Ser371) Antibody. The picture on the right is blocked with the phospho peptide.)

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p70 S6 Kinase, Polyclonal Antibody (Cat# AAA316202)

Full Name

p70 S6 Kinase (Phospho-Ser371) Antibody

Gene Names

RPS6KB1; S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from HT29 cells treated with serum 20% 15', using p70 S6 Kinase (Phospho-Ser418) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using p70 S6 Kinase (Phospho-Ser418) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of COS7 cells, using p70 S6 Kinase (Phospho-Ser418) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

p70 S6 Kinase, Polyclonal Antibody (Cat# AAA316204)

Full Name

p70 S6 Kinase (Phospho-Ser418) Antibody

Gene Names

RPS6KB1; S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from 293 cells treated with etoposide 25uM 1h, using PAK1 (Phospho-Thr212) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PAK1 (Phospho-Thr212) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

PAK1, Polyclonal Antibody (Cat# AAA316207)

Full Name

PAK1 (Phospho-Thr212) Antibody

Gene Names

PAK1; PAKalpha

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from Jurkat cells treated with UV 15', using PLCG2 (Phospho-Tyr1217) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using PLCG2 (Phospho-Tyr1217) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells, using PLCG2 (Phospho-Tyr1217) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

PLCG2, Polyclonal Antibody (Cat# AAA316213)

Full Name

PLCG2 (Phospho-Tyr1217) Antibody

Gene Names

PLCG2; FCAS3; APLAID; PLC-IV; PLC-gamma-2

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from A549 cells, using PP2A-alpha (Phospho-Tyr307) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using PP2A-alpha (Phospho-Tyr307) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells, using PP2A-alpha (Phospho-Tyr307) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

PP2A-alpha, Polyclonal Antibody (Cat# AAA316214)

Full Name

PP2A-alpha (Phospho-Tyr307) Antibody

Gene Names

PPP2CA; RP-C; PP2Ac; PP2CA; PP2Calpha

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from HUVEC cells treated with Serum 20% 30', using PPAR-BP (Phospho-Thr1457) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human heart, using PPAR-BP (Phospho-Thr1457) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells, using PPAR-BP (Phospho-Thr1457) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

PPAR-BP, Polyclonal Antibody (Cat# AAA316215)

Full Name

PPAR-BP (Phospho-Thr1457) Antibody

Gene Names

MED1; PBP; CRSP1; RB18A; TRIP2; PPARBP; CRSP200; DRIP205; DRIP230; PPARGBP; TRAP220

Reactivity

Human, Mouse

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from 293 cells treated with heat shock, using Progesterone Receptor (Phospho-Ser400) Antibody. The lane on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells, using Progesterone Receptor (Phospho-Ser400) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

Progesterone Receptor, Polyclonal Antibody (Cat# AAA316217)

Full Name

Progesterone Receptor (Phospho-Ser400) Antibody

Gene Names

PGR; PR; NR3C3

Reactivity

Human

Applications

Immunofluorescence, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from RAW264.7 cells treated with Calyculin 100nM 15', using Rabphilin 3A (Phospho-Ser237) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using Rabphilin 3A (Phospho-Ser237) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells, using Rabphilin 3A (Phospho-Ser237) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

Rabphilin 3A, Polyclonal Antibody (Cat# AAA316218)

Full Name

Rabphilin 3A (Phospho-Ser237) Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

What Are Phospho Antibodies?

Protein phosphorylation is a process where a phosphate group is added to certain amino acid residues of a protein – usually serine (S), threonine (T), or tyrosine (Y) - by enzymes called kinases. This process is integral in controlling cellular signaling, cellular growth, and other biological functions.

Our catalog includes a wide range of phospho-specific antibodies that can accurately detect this important marker. They perform strongly in widely-used laboratory applications such as Western blot, flow cytometry, immunohistochemistry, and immunofluorescence microscopy. We value your trust in us and are committed to providing top-quality products and services. All of our antibodies are guaranteed to work for the applications and species indicated on our website & associated product pages.

What Are The Key Applications of Phospho Antibodies?

1. Western Blotting

One of the first steps a researcher can take in utilizing these phospho-specific antibodies, is to check if the antibody works using a technique referred to as “Western blot”. For those unfamiliar, Western Blot aids in showing whether the protein that the antibody recognizes is appearing at the correct/expected size. These phospho-specific antibodies should also be able to detect changes in the target protein’s phosphorylation (on/off state) when cells are stimulated in certain ways.

2. Staining of Fixed Cells (Immunocytochemistry)

Another routine use of these phospho-specific antibodies, is to test if the antibody is able to demonstrate similar performance when used on fixed cells (intact cells that have been preserved) as it did in the Western blot tests. It is an important aspect in many cases to confirm that the antibody works in actual intact cell samples. Ideally, the method used for cellular fixation should be the same as what is used in pathology labs (like using 10% formalin). To check if the antibody works well in tissue sections (FFPE), researchers will often test it on fixed cells that are processed similar to tissue samples.

3. Specificity Tests Using Peptides

In order to make sure that the antibody is only binding to the right target:

• Laboratory technicians will mix the antibody with phospho-peptides (short segments of the protein containing the phosphate group modification).


• If the antibody signal disappears, it is confirmation that it is binding to the correct phosphorylated location.


• A more robust test is to use both the phosphorylated and non-phosphorylated (dephosphorylated) versions of the protein. The antibody should react only with the phosphorylated one.


• Another method sometimes utilized is to treat the sample with an enzyme, such as alkaline phosphatase, that specifically removes phosphate groups. If the antibody signal disappears after this, it also confirms specificity.

4. Genetic Confirmation

As a final step, scientists can genetically manipulate the nucleotide sequence and alter the target protein by removing the exact site where phosphorylation happens. If the antibody no longer appears to detect the modified protein, it is strong evidence supporting the antibody being specific for that phosphorylated site.

Why Buy Phospho Antibodies Through Us?

• The production laboratory adheres to strict and consistent protocols prior to releasing any of these phospho-specific antibodies:


• Standard methods and proper controls in all tests to ensure high quality.


• These antibodies are tested and validated in different cell types and species.


• High quality control criterion to ensure each batch is consistent, so you will obtain reliable results every time.

FAQ

1. What Are Phospho-Specific Antibodies?

Phospho-specific antibodies are made to detect proteins only when they have a phosphate group linked to a specific amino acid residue. This empowers scientists understand if a protein is "turned on" or active, based on its phosphorylation state.

2. How to Detect Phosphorylated Proteins in a Western Blot?

To find out if a protein is phosphorylated using Western blot:

• Use a phospho-specific antibody that binds only to the phosphorylated form of the protein.


• You can also use a “regular” antibody for the same amino acid sequence of the protein that the phospho-specific antibody is binding to (but in this case, this antibody will not bind if there is a phosphate group present) in order to compare how much of it is phosphorylated versus how much is non-phosphorylated (or “total” protein, if the “normal” antibody’s epitopes are non-phospho-site-specific).

3. How to Choose the Best Antibody?

Here are some simple tips to help you pick the right antibody:

• Know your target


• Match your sample characteristics


• Confirm the intended use is appropriate


• Check “host” and “type”


• Check the “quality” of the presented data/images


• Appraise whether the available validation meets your needs