Phospho Antibodies

Phospho-specific antibodies’ typical purpose is to enable researchers to detect changes in proteins. They will exclusively bind to the amino acid sequence on a protein that has been phosphorylated (which is both a physical & chemical change) and do not bind to the same amino acid sequence on said protein if it lacks said phosphorylation. This aids in being able to clearly see and understand the data produced from this particular protein modification.

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human skeletal muscle, using DAPK2 (Phospho-Ser318) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of COS7 cells, using DAPK2 (Phospho-Ser318) Antibody. The picture on the right is blocked with the phospho peptide.)

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DAPK2, Polyclonal Antibody (Cat# AAA316349)

Full Name

DAPK2 (Phospho-Ser318) Antibody

Gene Names

DAPK2; DRP1; DRP-1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from 293 cells treated with Insulin 0.01U/ml 2', using DAPP1 (Phospho-Tyr139) Antibody. The lane on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells, using DAPP1 (Phospho-Tyr139) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

DAPP1, Polyclonal Antibody (Cat# AAA316351)

Full Name

DAPP1 (Phospho-Tyr139) Antibody

Gene Names

DAPP1; BAM32

Reactivity

Human, Mouse

Applications

Immunofluorescence, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using DDX5/DEAD-box Protein 5 (Phospho-Tyr593) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells, using DDX5/DEAD-box Protein 5 (Phospho-Tyr593) Antibody. The picture on the right is blocked with the phospho peptide.)

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DDX5/DEAD-box Protein 5, Polyclonal Antibody (Cat# AAA316353)

Full Name

DDX5/DEAD-box Protein 5 (Phospho-Tyr593) Antibody

Gene Names

DDX5; p68; HLR1; G17P1; HUMP68

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using DNA-PK (Phospho-Thr2647) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HUVEC cells treated with serum 20% 30', using DNA-PK (Phospho-Thr2647) Antibody. The picture on the right is blocked with the phospho peptide.)

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DNA-PK, Polyclonal Antibody (Cat# AAA316355)

Full Name

DNA-PK (Phospho-Thr2647) Antibody

Gene Names

PRKDC; HYRC; p350; DNAPK; DNPK1; HYRC1; IMD26; XRCC7; DNA-PKcs

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HepG2 cells, using EPHA2/3 (Phospho-Tyr588/596) Antibody. The lane on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells, using EPHA2/3 (Phospho-Tyr588/596) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

EPHA2/3, Polyclonal Antibody (Cat# AAA316356)

Full Name

EPHA2/3 (Phospho-Tyr588/596) Antibody

Gene Names

EPHA2; ECK; CTPA; ARCC2; CTPP1; CTRCT6

Reactivity

Human, Rat

Applications

Immunofluorescence, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HeLa cells, using Estrogen Receptor-beta (Phospho-Ser105) Antibody. The lane on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HUVEC cells, using Estrogen Receptor-beta (Phospho-Ser105) Antibody. The picture on the right is blocked with the phospho peptide.)

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Estrogen Receptor-beta, Polyclonal Antibody (Cat# AAA316360)

Full Name

Estrogen Receptor-beta (Phospho-Ser105) Antibody

Gene Names

ESR2; Erb; ESRB; ESTRB; NR3A2; ER-BETA; ESR-BETA

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from Jurkat cells treated with Ca2+ 40nM 30', using FAK (Phospho-Tyr397) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using FAK (Phospho-Tyr397) Antibody. The picture on the right is blocked with the phospho peptide.)

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FAK, Polyclonal Antibody (Cat# AAA316363)

Full Name

FAK (Phospho-Tyr397) Antibody

Gene Names

PTK2; FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from 293 cells, using GATA4 (Phospho-Ser262) Antibody. The lane on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HUVEC cells, using GATA4 (Phospho-Ser262) Antibody. The picture on the right is blocked with the phospho peptide.)

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GATA4, Polyclonal Antibody (Cat# AAA316364)

Full Name

GATA4 (Phospho-Ser262) Antibody

Gene Names

GATA4; TOF; ASD2; VSD1; TACHD

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from 293 cells treated with UV 30', using BCL-XL (Phospho-Thr47) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using BCL-XL (Phospho-Thr47) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells, using BCL-XL (Phospho-Thr47) Antibody. The picture on the right is blocked with the phospho peptide.)

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BCL-XL, Polyclonal Antibody (Cat# AAA316294)

Full Name

BCL-XL (Phospho-Thr47) Antibody

Gene Names

BCL2L1; BCLX; BCL2L; Bcl-X; PPP1R52; BCL-XL/S

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from COS7 cells, using Bcr (Phospho-Tyr360) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using Bcr (Phospho-Tyr360) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells, using Bcr (Phospho-Tyr360) Antibody. The picture on the right is blocked with the phospho peptide.)

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Bcr, Polyclonal Antibody (Cat# AAA316295)

Full Name

Bcr (Phospho-Tyr360) Antibody

Gene Names

BCR; ALL; CML; PHL; BCR1; D22S11; D22S662

Reactivity

Human, Mouse

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HeLa cells treated with Serum 20% 15', using ETK (Phospho-Tyr566) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human thyroid gland, using ETK (Phospho-Tyr566) Antibody. The picture on the right is blocked with the phospho peptide.)

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ETK, Polyclonal Antibody (Cat# AAA316300)

Full Name

ETK (Phospho-Tyr566) Antibody

Gene Names

BMX; ETK; PSCTK2; PSCTK3

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from NIH/3T3 cells treated with PMA 250ng/ml 15', using PKC-pan (Phospho-Thr497) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PKC-pan (Phospho-Thr497) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HepG2 cells, using PKC-pan (Phospho-Thr497) Antibody. The picture on the right is blocked with the phospho peptide.)

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PKC-pan, Polyclonal Antibody (Cat# AAA316303)

Full Name

PKC-pan (Phospho-Thr497) Antibody

Gene Names

PRKCA; AAG6; PKCA; PRKACA; PKC-alpha

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from COS7 cells treated with UV 30', using PTP1B (Phospho-Ser50) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using PTP1B (Phospho-Ser50) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

PTP1B, Polyclonal Antibody (Cat# AAA316308)

Full Name

PTP1B (Phospho-Ser50) Antibody

Gene Names

PTPN1; PTP1B

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from mouse liver, using BAD (Phospho-Ser134) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using BAD (Phospho-Ser134) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells, using BAD (Phospho-Ser134) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

BAD, Polyclonal Antibody (Cat# AAA316315)

Full Name

BAD (Phospho-Ser134) Antibody

Gene Names

BAD; BBC2; BCL2L8

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from K562 cells treated with insulin 0.01U/ml 15' and Jurkat cells treated with insulin 0.01U/ml 15', using CaMK1-alpha (Phospho-Thr177) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using CaMK1-alpha (Phospho-Thr177) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells, using CaMK1-alpha (Phospho-Thr177) Antibody. The picture on the right is blocked with the phospho peptide.)

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CaMK1-alpha, Polyclonal Antibody (Cat# AAA316320)

Full Name

CaMK1-alpha (Phospho-Thr177) Antibody

Gene Names

CAMK1; CAMKI

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from K562 cells, using Caspase 9 (Phospho-Ser144) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using Caspase 9 (Phospho-Ser144) Antibody. The picture on the right is blocked with the phospho peptide.)

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Caspase 9, Polyclonal Antibody (Cat# AAA316323)

Full Name

Caspase 9 (Phospho-Ser144) Antibody

Gene Names

CASP9; MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from COS7 cells, using CD226/DNAM-1 (Phospho-Ser329) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using CD226/DNAM-1 (Phospho-Ser329) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells, using CD226/DNAM-1 (Phospho-Ser329) Antibody. The picture on the right is blocked with the phospho peptide.)

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CD226/DNAM-1, Polyclonal Antibody (Cat# AAA316327)

Full Name

CD226/DNAM-1 (Phospho-Ser329) Antibody

Gene Names

CD226; PTA1; DNAM1; DNAM-1; TLiSA1

Reactivity

Human, Mouse

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using CD4 (Phospho-Ser433) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HepG2 cells, using CD4 (Phospho-Ser433) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

CD4, Polyclonal Antibody (Cat# AAA316328)

Full Name

CD4 (Phospho-Ser433) Antibody

Gene Names

CD4; CD4mut

Reactivity

Human, Mouse

Applications

Immunofluorescence, Immunohistochemistry

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed A549 cells using ACK1 (Phospho-Tyr284) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ACK1 (Phospho-Tyr284) antibody (left)or the same antibody preincubated with blocking peptide (right).)

WB (Western Blot) (Western blot analysis of extracts from HepG2 cells treated with EGF using ACK1 (Phospho-Tyr284) Antibody.The lane on the right is treated with the antigen-specific peptide.)

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TNK2, Polyclonal Antibody (Cat# AAA243344)

Full Name

Phospho-TNK2 (Tyr284) Antibody

Gene Names

TNK2; ACK; ACK1; ACK-1; p21cdc42Hs

Reactivity

Human, Mouse

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using HER2 (Phospho-Tyr1248) Antibody.)

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed MCF7 cells using HER2(Phospho-Tyr1248) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HER2 (Phospho-Tyr1248) Antibody (left) or the same antibody preincubated with blocking peptide (right).)

WB (Western Blot) (Western blot analysis of extracts from HepG2 cells treated with PMA and MCF cells treated with EGF, using HER2 (Phospho-Tyr1248) Antibody.)

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ERBB2, Polyclonal Antibody (Cat# AAA243357)

Full Name

Phospho-ERBB2 (Tyr1248) Antibody

Gene Names

ERBB2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts from PC12 cells, treated with PMA or calf intestinal phosphatase (CIP), using PKCtheta (Phospho-Ser676) Antibody.)

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using PKCth(Phospho-Ser676) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PKCth(Phospho-Ser676) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from PC12 cells untreated or treated with PMA using PKCth(Phospho-Ser676) Antibody.)

Compare (5 max)

PRKCQ, Polyclonal Antibody (Cat# AAA243371)

Full Name

Phospho-PRKCQ (Ser676) Antibody

Gene Names

PRKCQ; PRKCT; nPKC-theta

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using 4E-BP1 (phospho-Thr36) antibody.)

WB (Western Blot) (Western blot analysis of extracts from MDA-MB-435 cells, untreated or EGF-treated (200 ng/ml, 30min), using 4E-BP1 (Ab-36) antibody (Lane 1 and 2) and 4E-BP1 (phospho-Thr36) antibody (Lane 3 and 4).)

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EIF4EBP1, Polyclonal Antibody (Cat# AAA243178)

Full Name

Phospho-EIF4EBP1 (Thr36) Antibody

Gene Names

EIF4EBP1; BP-1; 4EBP1; 4E-BP1; PHAS-I

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using p130 Cas (Phospho-Tyr410) antibody (left)or the same antibody preincubated with blocking peptide (right).)

WB (Western Blot) (Western blot analysis of extracts from K562 cells (Lane 2) and 3T3 cells (Lane 3), using P130 Cas(Phospho-Tyr410) Antibody. The lane on the left is treated with antigen-specific peptide.)

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BCAR1, Polyclonal Antibody (Cat# AAA243185)

Full Name

Phospho-BCAR1 (Tyr410) Antibody

Gene Names

BCAR1; CAS; CAS1; CASS1; CRKAS; P130Cas

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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ChIP (Chromatin Immunoprecipitation) (AbFlex RNA Pol II CTD phospho Tyr1 recombinant antibody (rAb) tested by ChIP-Seq Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 30 ug of Raji cell chromatin and 10 ug of antibody. ChIP DNA was sequenced on the Illumina NextSeq and 17.3 million sequence tags were mapped to identify RNA Pol II CTD phosphor Tyr1 binding sites on chromosome 12.)

IF (Immunofluorescence) (AbFlex RNA Pol II CTD phospho Tyr1 antibody (rAb) tested by Immunofluorescence. HeLa cells were stained with 2 ug /mL of AbFlex RNA Pol II CTD phospho Tyr1 antibody (rAb) followed by anti-mouse IgG-488.)

Application Data (AbFlex RNA pol II CTD phospho Tyr1 antibody (rAb) tested by Luminex bead-based specificity analysis. Luminex bead-based specificity analysis was used to confirm the specificity of AbFlex RNA pol II CTD phospho Tyr1 antibody (rAb) antibody for RNA pol II CTD peptides. RNA pol II peptides were conjugated to MagPlex Luminex beads and incubated with 500 ng of AbFlex RNA pol II CTD phospho Tyr1 antibody (rAb). Peptide-bound antibody was detected with anti-mouse IgG-Phycoerythrin (PE) and read in a Luminex instrument. Luminex is a registered trademark of Luminex Corporation.)

SDS-PAGE (AbFlex RNA Pol II CTD phospho Tyr1 antibody (rAb) (rAb) tested by Western Blot. 20 ug of HeLa cell nuclear extract was run on SDS-PAGE and probed with AbFlex RNA Pol II CTD phospho Tyr1 antibody at 2 ug/ml.)

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RNA Pol II CTD phospho Tyr1, Antibody (Cat# AAA60276)

Full Name

AbFlex RNA Pol II CTD phospho Tyr1 antibody (rAb)

Gene Names

POLR2A; RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220

Reactivity

Human

Applications

Western Blot, Immunofluorescence, Chromatin immunoprecipitation

Purity

Protein A Chromatography

Pricing

$205/0.01 mg $575/0.1 mg $1,110/2x0.1 mg $1,620/3x0.1 mg $2,105/4x0.1 mg $2,395/5x0.1 mg

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DB (Dot Blot) (STAT3 phospho Tyr705 pAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of STAT3 phospho Tyr705 pAb for STAT3 phospho Tyr705. Phosphorylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at 1:10,000. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: Unmodified Ser727 STAT1 peptide. Lane 2: Phospho Ser727 STAT1 peptide. Lane 3: Unmodified Tyr689 STAT2 peptide. Lane 4: Phospho Tyr689 STAT2 peptide. Lane 5: Unmodified Ser727 STAT3 peptide. Lane 6: Phospho Ser727 STAT3 peptide. Lane 7: Unmodified Tyr705 STAT3 peptide. Lane 8: Phospho Tyr705 STAT3 peptide. Lane 9: Unmodified Ser726 STAT5A/Ser731 STAT5B peptide. Lane 10: Phospho Ser726 STAT5A/Ser731 STAT5B peptide. Lane 11: Unmodified Tyr694 STAT5A/Tyr699 STAT5B peptide. Lane 12: Phospho Tyr694 STAT5A/Tyr699 STAT5B peptide.)

WB (Western Blot) (STAT3 phospho Tyr705 pAb tested by Western blot. Hep G2 nuclear extract (15 ug per lane) was probed with STAT3 phospho Tyr705 polyclonal antibody (1:2,000). Lane 1: No treatment. Lane 2: cells stimulated with IL-6 (100 ng/ml for 15 minutes at 37 degree C).)

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STAT3 phospho Tyr705, Polyclonal Antibody (Cat# AAA59891)

Full Name

STAT3 phospho Tyr705 antibody (pAb)

Gene Names

STAT3; APRF; HIES; ADMIO

Reactivity

Human

Applications

Western Blot, Dot Blot

Pricing

$250/0.01 mL $705/0.2 mL $1,375/2x0.2 mL $2,005/3x0.2 mL $2,605/4x0.2 mL $3,015/5x0.2 mL

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DB (Dot Blot) (HP1 gamma phospho Ser93 pAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of HP1 gamma phospho Ser93 pAb for HP1 gamma phospho Ser93. Phosphorylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with HP1 gamma phospho Ser93 pAb at 1:5,000. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: unmodified HP1 gamma peptide. Lane 2: phospho Ser93 HP1 gamma peptide. Lane 3: phospho Ser92 HP1 alpha peptide. Lane 4: phospho Ser89 HP1 beta peptide.)

WB (Western Blot) (HP1 gamma phospho Ser93 pAb tested by Western blot. HeLa nuclear extract (20 ug per lane) was probed with HP1 gamma phospho Ser93 pAb (1:500 dilution). Lane 1: no treatment. Lane 2: cells treated with colcemid.)

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HP1 gamma phospho Ser93, Polyclonal Antibody (Cat# AAA59908)

Full Name

HP1 gamma phospho Ser93 antibody (pAb)

Gene Names

CBX3; HECH; HP1-GAMMA; HP1Hs-gamma

Reactivity

Human

Applications

Western Blot

Purity

Affinity Purified

Pricing

$250/0.01 mL $705/0.1 mL $1,375/2x0.1 mL $2,005/3x0.1 mL $2,605/4x0.1 mL $3,015/5x0.1 mL

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DB (Dot Blot) (RNA pol II CTD phospho Ser5 pAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of RNA pol II CTD phospho Ser5 antibody for phospho-Ser5 of the RNA Pol II C-terminal domain heptad repeat. Modified and unmodified peptides were spotted onto PVDF and probed with the antibody at a dilution of 1:1,000. Decreasing amounts of peptide were spotted in each row. Lane 1: Peptide phosphorylated at CTD repeat serine 2. Lane 2: Unmodified CTD repeat serine 2 peptide. Lane 3: Peptide phosphorylated at CTD repeat serine 5. Lane 4: Unmodified CTD repeat serine 5 peptide.)

WB (Western Blot) (RNA pol II CTD phospho Ser5 antibody tested by Western blot. Nuclear extract of HeLa cells (20 ug) probed with RNA pol II CTD phospho Ser5 antibody at a 1:2,000 dilution.)

IF (Immunofluorescence) (RNA pol II CTD phospho Ser5 antibody tested by immunofluorescence. Top image: HeLa cells stained with RNA pol II CTD phospho Ser5 antibody at a 1:1,000 dilution. Middle: Same cells stained with DAPI. Bottom: Merge of both images.)

ChIP (Chromatin Immunoprecipitation) (RNA pol II CTD phospho Ser5 antibody tested by ChIP analysis. Chromatin IP performed using the ChIP-IT Express Kit and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 10 ul of RNA pol II CTD phospho Ser5 antibody or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the PABPC1 gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.)

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RNA pol II CTD phospho Ser5, Polyclonal Antibody (Cat# AAA59848)

Full Name

RNA pol II CTD phospho Ser5 antibody (pAb)

Gene Names

POLR2A; RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220

Reactivity

Human, Mouse, Wide Range Predicted

Applications

Western Blot, Immunofluorescence, Immunocytochemistry, Chromatin immunoprecipitation

Pricing

$250/0.01 mL $725/0.1 mL $1,415/2x0.1 mL $2,070/3x0.1 mL $2,680/4x0.1 mL $3,100/5x0.1 mL

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DB (Dot Blot) (RNA Pol II CTD phospho Thr4 pAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of RNA Pol II CTD phospho Thr4 pAb. Peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at a dilution of 1 ug/ml. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: Phospho Thr4 of RNA Pol II CTD peptide. Lane 2: Unmodified Thr4 of RNA Pol II CTD peptide. Lane 3: Phospho Ser2 of RNA Pol II CTD peptide. Lane 4: Phospho Ser5 of RNA Pol II CTD peptide.)

WB (Western Blot) (RNA pol II CTD phospho Thr4 antibody pAb tested by Western blot. Nuclear extract of HeLa cells (40 ug) probed with RNA pol II CTD phospho Thr4 antibody at a dilution of 2 ug/ml.)

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RNA pol II CTD phospho Thr4, Polyclonal Antibody (Cat# AAA60008)

Full Name

RNA pol II CTD phospho Thr4 antibody (pAb)

Gene Names

POLR2A; RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220

Reactivity

Human

Applications

Western Blot

Purity

Protein A Chromatography

Pricing

$250/0.01 mg $705/0.1 mg $1,375/2x0.1 mg $2,005/3x0.1 mg $2,605/4x0.1 mg $3,015/5x0.1 mg

WB (Western Blot) (The whole cell lysate derived from human umbilical vein endothelial cell was stimulated by VEGF-A for 20 min, then immunoprecipitated by Rabbit anti-VEGFR-2 followed by immune-probing with Rabbit anti phosphor-VEGFR-2 (pY951) (AAA71420) at 1:500. An immunoreactive band is observed around ~240kDa(lane 1). The lane 2 is a negative control.)

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VEGFR-2 (pY951) (CD309), Antibody (Cat# AAA71420)

Full Name

Rabbit anti Phospho-VEGFR-2 (pTyr951)

Gene Names

KDR; FLK1; CD309; VEGFR; VEGFR2

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

The Rabbit IgG is purified by Site-specific Epitope Affinity Purification.

Pricing

$365/0.1 mg $705/2x0.1 mg $1,030/3x0.1 mg $1,335/4x0.1 mg $1,420/5x0.1 mg

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human breast carcinoma using Phospho-STMN1-S25 antibody.)

WB (Western Blot) (Western blot analysis of extracts of Jurkat cells, using Phospho-STMN1-S25 antibody at 1:2000 dilution. Jurkat cells were treated by PMA/TPA (200nM) for 10 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 10s.)

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STMN1-S25, Antibody (Cat# AAA37416)

Full Name

Phospho-STMN1-S25 Polyclonal Antibody

Gene Names

STMN1; Lag; SMN; OP18; PP17; PP19; PR22; LAP18; C1orf215

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity Purification

Pricing

$250/0.02 mL $475/0.1 mL $925/2x0.1 mL $1,355/3x0.1 mL $1,755/4x0.1 mL $1,935/5x0.1 mL

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Application Data

IF (Immunofluorescence)

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GATA1, Polyclonal Antibody (Cat# AAA47822)

Full Name

GATA1 (phospho-Ser310)

Gene Names

GATA1; GF1; GF-1; NFE1; XLTT; ERYF1; NF-E1; XLANP; XLTDA; GATA-1

Applications

Immunofluorescence, Immunoblot

Pricing

$475/0.1 mL $925/2x0.1 mL $1,355/3x0.1 mL $1,755/4x0.1 mL $1,980/5x0.1 mL

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Application Data (Detection of CREB (phospho-Ser133) in HeLa cells extracts untreated or treated with UV.These are recommended working dilutions. Enduser should determine optimal dilutions for their applications.)

IHC (Immunohistochemistry) (Detection of CREB (phospho-Ser133) in paraffin-embedded human breast carcinoma tissue.)

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CREB, Polyclonal Antibody (Cat# AAA47831)

Full Name

CREB (phospho-Ser133)

Gene Names

CREB1; CREB; CREB-1

Applications

Immunohistochemistry, Western Blot

Pricing

$475/0.1 mL $925/2x0.1 mL $1,355/3x0.1 mL $1,755/4x0.1 mL $1,980/5x0.1 mL

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Application Data

IHC (Immunohistochemistry)

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BAD, Polyclonal Antibody (Cat# AAA47842)

Full Name

BAD (Phospho-Ser155)

Gene Names

Bad; Bbc2; AI325008

Applications

Immunohistochemistry, Immunoblot

Pricing

$475/0.1 mL $925/2x0.1 mL $1,355/3x0.1 mL $1,755/4x0.1 mL $1,980/5x0.1 mL

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Application Data

IHC (Immunohiostchemistry)

IF (Immunofluorescence)

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Estrogen Receptor-a, Polyclonal Antibody (Cat# AAA47845)

Full Name

Estrogen Receptor-a (Phospho-Ser118)

Gene Names

ESR1; ER; ESR; Era; ESRA; ESTRR; NR3A1

Applications

Immunohistochemistry, Immunofluorescence, Immunoblot

Pricing

$475/0.1 mL $925/2x0.1 mL $1,355/3x0.1 mL $1,755/4x0.1 mL $1,980/5x0.1 mL

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Application Data

IHC (Immunohistochemistry)

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GSK3alpha, Polyclonal Antibody (Cat# AAA47791)

Full Name

GSK3alpha (Phospho-Ser21)

Applications

Immunohistochemistry, Western Blot

Pricing

$475/0.1 mL $925/2x0.1 mL $1,355/3x0.1 mL $1,755/4x0.1 mL $1,980/5x0.1 mL

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IHC (Immunohiostchemistry)

IF (Immunofluorescence)

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NFkB-p105, Polyclonal Antibody (Cat# AAA47802)

Full Name

NFkB-p105 (Phospho-Ser893)

Gene Names

NFKB1; p50; KBF1; p105; EBP-1; CVID12; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B

Applications

Immunofluorescence, Immunohistochemistry

Pricing

$475/0.1 mL $925/2x0.1 mL $1,355/3x0.1 mL $1,755/4x0.1 mL $1,980/5x0.1 mL

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Application Data

IHC (Immunohistochemistry)

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JunB, Polyclonal Antibody (Cat# AAA47809)

Full Name

JunB (Phospho-Ser259)

Gene Names

JUNB; AP-1

Applications

Immunohistochemistry, Immunoblot

Pricing

$475/0.1 mL $925/2x0.1 mL $1,355/3x0.1 mL $1,755/4x0.1 mL $1,980/5x0.1 mL

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Application Data

IHC (Immunohiostchemistry)

IF (Immunofluorescence)

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Myc, Polyclonal Antibody (Cat# AAA47817)

Full Name

Myc (phospho-Thr358)

Gene Names

MYC; MRTL; MYCC; c-Myc; bHLHe39

Applications

Immunofluorescence, Immunohistochemistry, Immunoblot

Pricing

$475/0.1 mL $925/2x0.1 mL $1,355/3x0.1 mL $1,755/4x0.1 mL $1,980/5x0.1 mL

Standard Curve (Sample)

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Phospho protein kinase C, ELISA Kit (Cat# AAA84307)

Full Name

Rat Phospho protein kinase C ELISA Kit

Reactivity

Rat

Pricing

$595/48-Strip-Wells $845/96-Strip-Wells $1,640/2x96-Strip-Wells $3,715/5x96-Strip-Wells $7,005/10x96-Strip-Wells

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-AKT1 (T450) antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Phospho-AKT1 (T450) on MCF-7 cells lysates using anti-Phospho-AKT1 (T450) antibody at 1/1, 000 dilution.)

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AKT1, Monoclonal Antibody (Cat# AAA311034)

Full Name

AKT1 (Phospho-T450) Antibody

Gene Names

AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

ProA affinity purified

Pricing

$365/0.05 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $1,990/5x0.1 mL

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ICC (Immunocytochemistry) (ICC staining phospho-ATF2 (T71) in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining phospho-ATF2 (T71) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining phospho-ATF2 (T71) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

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ATF2, Monoclonal Antibody (Cat# AAA311012)

Full Name

ATF2 (Phospho-T71) Antibody

Gene Names

LRP1; APR; LRP; A2MR; CD91; APOER; LRP1A; TGFBR5; IGFBP3R

Reactivity

Human

Applications

Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Western Blot

Purity

ProA affinity purified

Pricing

$365/0.05 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $1,990/5x0.1 mL

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IHC (Immunohistochemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot) (The phospho-STAT3-Ser727 Pab is used in Western blot to detect Ser727-phosphorylated STAT3 in HL-60 tissue lysates collected before (-) or after (+) stimulation obtained by IFN-a.)

WB (Western Blot) (The Phospho-STAT3-S727 Pab is used in western blot to detect Ser727-phosphorylated STAT3 in mouse liver tissue lysates collected before (-) or after (+) stimulation obtained by IL-6 injection in mouse portal vein. Data is kindly provided by Drs. E. Bard-Chapeau and G-S. Feng from the Burnham Institute (La Jolla, CA).)

IF (Immunofluorescence) (Fluorescent confocal image of SY5Y cells stained with phospho-STAT3-S727 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AAA288729 phospho-STAT3-S727 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the phospho-STAT3 immunosignal mainly to the nucleus, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000168610).)

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Phospho-STAT3 (S727), Polyclonal Antibody (Cat# AAA288729)

Full Name

Phospho-STAT3 (S727) Antibody

Gene Names

STAT3; APRF; HIES; ADMIO

Reactivity

Human, mouse

Applications

Immunohistochemistry, Western Blot, Immunofluorescence

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human Skin Tissue using Phospho-MLKL S358 Mouse mAb diluted at 1:200.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma Tissue using Phospho-MLKL S358 Mouse mAb diluted at 1:200.)

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phospho-MLKL, Monoclonal Antibody (Cat# AAA309604)

Full Name

phospho-MLKL (S358) Mouse Monoclonal Antibody (6F8)

Reactivity

Human

Applications

Immunohistochemistry

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts from cos-7 cells (Lane 2), using ITGB1 (Phospho-Tyr795) Antibody. The lane on the left is treated with synthesized peptide.)

WB (Western Blot) (Western blot analysis of extracts from HeLa cells treated with H2O2, using ITGB1 (Phospho-Tyr795) antibody.)

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ITGB1 (Phospho-Tyr795), Polyclonal Antibody (Cat# AAA289951)

Full Name

ITGB1 (Phospho-Tyr795) Antibody

Gene Names

ITGB1; CD29; FNRB; MDF2; VLAB; GPIIA; MSK12; VLA-BETA

Applications

Western Blot

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT6(Phospho-Tyr641) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from Hela cells untreated or treated with IL-4 using STAT6(Phospho-Tyr641) Antibody.)

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STAT6, Polyclonal Antibody (Cat# AAA243059)

Full Name

Phospho-STAT6 (Tyr641) Antibody

Gene Names

STAT6; STAT6B; STAT6C; D12S1644; IL-4-STAT

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

What Are Phospho Antibodies?

Protein phosphorylation is a process where a phosphate group is added to certain amino acid residues of a protein – usually serine (S), threonine (T), or tyrosine (Y) - by enzymes called kinases. This process is integral in controlling cellular signaling, cellular growth, and other biological functions.

Our catalog includes a wide range of phospho-specific antibodies that can accurately detect this important marker. They perform strongly in widely-used laboratory applications such as Western blot, flow cytometry, immunohistochemistry, and immunofluorescence microscopy. We value your trust in us and are committed to providing top-quality products and services. All of our antibodies are guaranteed to work for the applications and species indicated on our website & associated product pages.

What Are The Key Applications of Phospho Antibodies?

1. Western Blotting

One of the first steps a researcher can take in utilizing these phospho-specific antibodies, is to check if the antibody works using a technique referred to as “Western blot”. For those unfamiliar, Western Blot aids in showing whether the protein that the antibody recognizes is appearing at the correct/expected size. These phospho-specific antibodies should also be able to detect changes in the target protein’s phosphorylation (on/off state) when cells are stimulated in certain ways.

2. Staining of Fixed Cells (Immunocytochemistry)

Another routine use of these phospho-specific antibodies, is to test if the antibody is able to demonstrate similar performance when used on fixed cells (intact cells that have been preserved) as it did in the Western blot tests. It is an important aspect in many cases to confirm that the antibody works in actual intact cell samples. Ideally, the method used for cellular fixation should be the same as what is used in pathology labs (like using 10% formalin). To check if the antibody works well in tissue sections (FFPE), researchers will often test it on fixed cells that are processed similar to tissue samples.

3. Specificity Tests Using Peptides

In order to make sure that the antibody is only binding to the right target:

• Laboratory technicians will mix the antibody with phospho-peptides (short segments of the protein containing the phosphate group modification).


• If the antibody signal disappears, it is confirmation that it is binding to the correct phosphorylated location.


• A more robust test is to use both the phosphorylated and non-phosphorylated (dephosphorylated) versions of the protein. The antibody should react only with the phosphorylated one.


• Another method sometimes utilized is to treat the sample with an enzyme, such as alkaline phosphatase, that specifically removes phosphate groups. If the antibody signal disappears after this, it also confirms specificity.

4. Genetic Confirmation

As a final step, scientists can genetically manipulate the nucleotide sequence and alter the target protein by removing the exact site where phosphorylation happens. If the antibody no longer appears to detect the modified protein, it is strong evidence supporting the antibody being specific for that phosphorylated site.

Why Buy Phospho Antibodies Through Us?

• The production laboratory adheres to strict and consistent protocols prior to releasing any of these phospho-specific antibodies:


• Standard methods and proper controls in all tests to ensure high quality.


• These antibodies are tested and validated in different cell types and species.


• High quality control criterion to ensure each batch is consistent, so you will obtain reliable results every time.

FAQ

1. What Are Phospho-Specific Antibodies?

Phospho-specific antibodies are made to detect proteins only when they have a phosphate group linked to a specific amino acid residue. This empowers scientists understand if a protein is "turned on" or active, based on its phosphorylation state.

2. How to Detect Phosphorylated Proteins in a Western Blot?

To find out if a protein is phosphorylated using Western blot:

• Use a phospho-specific antibody that binds only to the phosphorylated form of the protein.


• You can also use a “regular” antibody for the same amino acid sequence of the protein that the phospho-specific antibody is binding to (but in this case, this antibody will not bind if there is a phosphate group present) in order to compare how much of it is phosphorylated versus how much is non-phosphorylated (or “total” protein, if the “normal” antibody’s epitopes are non-phospho-site-specific).

3. How to Choose the Best Antibody?

Here are some simple tips to help you pick the right antibody:

• Know your target


• Match your sample characteristics


• Confirm the intended use is appropriate


• Check “host” and “type”


• Check the “quality” of the presented data/images


• Appraise whether the available validation meets your needs