Phospho Antibodies

Phospho-specific antibodies’ typical purpose is to enable researchers to detect changes in proteins. They will exclusively bind to the amino acid sequence on a protein that has been phosphorylated (which is both a physical & chemical change) and do not bind to the same amino acid sequence on said protein if it lacks said phosphorylation. This aids in being able to clearly see and understand the data produced from this particular protein modification.

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed HeLa cells using Phospho-MAPT-S262 antibody.)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded rat hippocampal region tissue from a model with Alzheimer using Phospho-MAPT-S262 antibody.)

WB (Western Blot) (Western blot analysis of extracts from mouse brain tissue using Phospho-MAPT-S262 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)

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MAPT-S262, Antibody (Cat# AAA37417)

Full Name

Phospho-MAPT-S262 Polyclonal Antibody

Gene Names

MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity Purification

Pricing

$250/0.02 mL $475/0.1 mL $925/2x0.1 mL $1,355/3x0.1 mL $1,755/4x0.1 mL $1,935/5x0.1 mL

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WB (Western Blot) (Western blot analysis of Phospho-Cdk1/2 (T14) expression in HeLa cell lysate treated with hydroxyurea (AAA124529).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CDK1)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat spleen, using Phospho-Cdk1/2 (T14) Antibody(AAA124529)CDK1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CDK1 Antibody (AAA124529)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

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Cdk1/2, Monoclonal Antibody (Cat# AAA124529)

Full Name

Anti-Phospho-Cdk1/2 (T14) Rabbit Monoclonal Antibody

Gene Names

CDK1; CDC2; CDC28A; P34CDC2

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunoprecipitation

Purity

Affinity-chromatography

Pricing

$215/0.03 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,005/5x0.1 mL

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ICC (Immunocytochemistry) (ICC staining ATM (phospho S1981) in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining ATM (phospho S1981) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining ATM (phospho S1981) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-ATM (phospho S1981) antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-ATM (phospho S1981) antibody. Counter stained with hematoxylin.)

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ATM, Monoclonal Antibody (Cat# AAA311046)

Full Name

ATM (Phospho-S1981) Antibody

Gene Names

ATM; AT1; ATA; ATC; ATD; ATE; ATDC; TEL1; TELO1

Reactivity

Human, Mouse, Rat

Applications

Immunoprecipitation, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Western Blot

Purity

ProA affinity purified

Pricing

$365/0.05 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $1,990/5x0.1 mL

WB (Western Blot) (FG Pancreatic Carcinoma Cell Lines stably expressing vector along (FG-V) the b3 integrin subunit (FG-b3) or a b3 truncation mutant (FG-759x).Phospho-Src-Y215 Antibody (AAA284306) was diluted 1:500 in 1% BSA/TBST and incubated Overnight at 4)

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Phospho-SRC (Y215), Polyclonal Antibody (Cat# AAA284306)

Full Name

Phospho-SRC (Y215) Antibody

Gene Names

SRC; ASV; SRC1; c-SRC; p60-Src

Reactivity

Human (Predicted Reactivity: Mouse, Rat, Zebrafish, Chicken, Xenopus)

Applications

Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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WB (Western Blot) (Western blot analysis of ERK1/2 (arrow) using rabbit polyclonal P-MAPK(T202/Y204). HBEpC cell lysates either transiently induced (Lane 2) or noninduced with the EGF (Lane 1)(2 ug/lane).)

WB (Western Blot) (Western blot analysis of ERK1/2 (arrow) using rabbit polyclonal P-MAPK(T202/Y204). HAOSMC cell lysates either transiently induced (Lane 2) or noninduced with the PDGF (Lane 1)(2 ug/lane).)

DB (Dot Blot) (Dot blot analysis of Bi-phospho-ERK1/2-T202/Y204 Antibody on nitrocellulose membrane. 50ng of Bisphospho-peptide or Non Phosphorylated peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

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Bi-Phospho-ERK1/2 (T202/Y204), Polyclonal Antibody (Cat# AAA288905)

Full Name

Bi-Phospho-ERK1/2 (T202/Y204) Antibody

Gene Names

MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK

Reactivity

Human (Predicted Reactivity: Drosophila, Mouse, Rat)

Applications

Western Blot, Dot Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot) (Western blot analysis of Phospho-NFKB-S536(M) in mouse kidney tissue lysates (35ug/lane). NFKB (arrow) was detected using the purified Pab.)

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Phospho-NFKB (S536), Polyclonal Antibody (Cat# AAA288691)

Full Name

Phospho-NFKB (S536) Antibody

Gene Names

RELA; p65; NFKB3

Reactivity

Human, mouse

Applications

Immunohistochemistry, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

DB (Dot Blot) (Dot blot analysis of Phospho-PARP1-S782 Antibody Phospho-specific Pab (AAA288409) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)

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Phospho-PARP1 (S782), Polyclonal Antibody (Cat# AAA288409)

Full Name

Phospho-PARP1 (S782) Antibody

Gene Names

PARP1; PARP; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1

Reactivity

Human (Predicted Reactivity: Bovine, Hamster, Mouse, Rat)

Applications

Dot Blot

Purity

This antibody is purified through a protein A column, followed by peptide affinity purification.

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot) (Western blot analysis of hTNFR-pS274 in MDA-MB468 cell line lysates (35ug/lane). TNFR (arrow) was detected using the purified Pab.)

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Phospho-TNFR (S274), Polyclonal Antibody (Cat# AAA288461)

Full Name

Phospho-TNFR (S274) Antibody

Gene Names

TNFRSF1A; FPF; MS5; p55; p60; TBP1; TNF-R; TNFAR; TNFR1; p55-R; CD120a; TNFR55; TNFR60; TNF-R-I; TNF-R55; TNFR1-d2

Reactivity

Human, mouse

Applications

Immunohistochemistry, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

DB (Dot Blot) (Dot blot analysis of mouse TSC2 Antibody (Phospho S1343) Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)

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Phospho-mouse TSC2 (S1343), Polyclonal Antibody (Cat# AAA288358)

Full Name

Phospho-mouse TSC2 (S1343) Antibody

Reactivity

Mouse (Predicted Reactivity: Rat)

Applications

Dot Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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DB (Dot Blot) (Dot blot analysis of Phospho-mouse ERBB2-Y1140 Antibody Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)

WB (Western Blot) (Western blot analysis of extracts from SK-BR-3 cell,untreated or treated with NRG,using phospho-mouse ERBB2-Y1140(left) or mouse ERBB2 antibody(right).)

WB (Western Blot) (Western blot analysis of lysates from A431 cell line, untreated or treated with EGF, 100ng/ml, using Phospho-mouse ERBB2-Y1140 (upper) or Beta-actin (lower).)

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Phospho-M ERBB2 (Y1140), Polyclonal Antibody (Cat# AAA288307)

Full Name

Phospho-M ERBB2 (Y1140) Antibody

Gene Names

Erbb2; Neu; HER2; HER-2; c-neu; Erbb-2; c-erbB2; mKIAA3023

Reactivity

Human, mouse

Applications

Dot Blot, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

DB (Dot Blot) (Dot blot analysis of anti-EIF4EBP1-pS111 Phospho-specific Pab (RB13351) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

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Phospho-EIF4EBP1 (S111), Polyclonal Antibody (Cat# AAA288214)

Full Name

Phospho-EIF4EBP1 (S111) Antibody

Gene Names

EIF4EBP1; BP-1; 4EBP1; 4E-BP1; PHAS-I

Reactivity

Human (Predicted Reactivity: Mouse, Rat)

Applications

Dot Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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IF (Immunofluorescence) (Confocal immunofluorescent analysis of Phospho-TSC2-S1420 Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)

DB (Dot Blot) (Dot blot analysis of anti-TSC2-pS1420 Phospho-specific Pab (RB13391) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

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Phospho-TSC2 (S1420), Polyclonal Antibody (Cat# AAA288234)

Full Name

Phospho-TSC2 (S1420) Antibody

Gene Names

TSC2; LAM; TSC4; PPP1R160

Reactivity

Human

Applications

Immunofluorescence, Dot Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4 degree overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.)

IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4 degree overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.)

ELISA (Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using p70 S6 Kinase (Phospho-Thr421) Antibody)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast cancer, using p70 S6 Kinase (Phospho-Thr421) Antibody. The picture on the right is blocked with the p70 S6 Kinase (Phospho-Thr421) peptide.)

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p70 S6 kinase alpha, Polyclonal Antibody (Cat# AAA318647)

Full Name

p70 S6 kinase alpha (Phospho-Thr421) Rabbit Polyclonal Antibody

Gene Names

RPS6KB1; S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

WB (Western Blot) (Western Blot analysis of various cells using Phospho-NFkappaB-p65 (S529) Polyclonal Antibody diluted at 1:500)

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NFkappaB-p65, ELISA Kit (Cat# AAA319182)

Full Name

NFkappaB-p65 (Phospho-Ser529) Colorimetric Cell-Based ELISA Kit

Gene Names

RELA; p65; NFKB3

Reactivity

Human, Mouse, Rat, Monkey

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from DU145 cells, primary antibody was diluted at 1:1000, 4 degree C over night)

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FRA1, ELISA Kit (Cat# AAA319105)

Full Name

FRA1 (Phospho-Ser265) Colorimetric Cell-Based ELISA Kit

Gene Names

FOSL1; FRA; FRA1; fra-1

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of HEPG2-UV MOUSE-BRAIN using p-EDG-1 (T236) antibody. Antibody was diluted at 1:500)

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EDG-1, ELISA Kit (Cat# AAA319166)

Full Name

EDG-1 (Phospho-Thr236) Colorimetric Cell-Based ELISA Kit

Gene Names

S1PR1; EDG1; S1P1; CD363; ECGF1; EDG-1; CHEDG1; D1S3362

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of 293 cells using Phospho-PKC (T497) Polyclonal Antibody diluted at 1:500)

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PKC, ELISA Kit (Cat# AAA319273)

Full Name

PKC (Phospho-Thr497) Colorimetric Cell-Based ELISA Kit

Gene Names

PRKCA; AAG6; PKCA; PRKACA; PKC-alpha

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

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WB (Western Blot) (Western blot analysis of lysates from A549 cells treated with UV 15', using CDC25A (Phospho-Ser178) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using CDC25A (Phospho-Ser178) Antibody. The picture on the right is blocked with the phospho peptide.)

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CDC25A, Polyclonal Antibody (Cat# AAA316597)

Full Name

CDC25A (Phospho-Ser178) Antibody

Gene Names

CDC25A; CDC25A2

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from Jurkat cells treated with PMA 125ng/ml 30', using Retinoblastoma (Phospho-Ser608) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using Retinoblastoma (Phospho-Ser608) Antibody. The picture on the right is blocked with the phospho peptide.)

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Retinoblastoma, Polyclonal Antibody (Cat# AAA316602)

Full Name

Retinoblastoma (Phospho-Ser608) Antibody

Gene Names

RB1; RB; pRb; OSRC; pp110; p105-Rb; PPP1R130

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from COS7 cells treated with nocodazole 1ug/ml 16h, using STMN1 (Phospho-Ser62) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using STMN1 (Phospho-Ser62) Antibody. The picture on the right is blocked with the phospho peptide.)

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STMN1, Polyclonal Antibody (Cat# AAA316604)

Full Name

STMN1 (Phospho-Ser62) Antibody

Gene Names

STMN1; Lag; SMN; OP18; PP17; PP19; PR22; LAP18; C1orf215

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from mouse brain, using Trk A (Phospho-Tyr701) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using Trk A (Phospho-Tyr701) Antibody. The picture on the right is blocked with the phospho peptide.)

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Trk A, Polyclonal Antibody (Cat# AAA316605)

Full Name

Trk A (Phospho-Tyr701) Antibody

Gene Names

NTRK1; MTC; TRK; TRK1; TRKA; Trk-A; p140-TrkA

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HT29 cells treated with heat shock, using CK-1 alpha (Phospho-Tyr294) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using CK-1 alpha (Phospho-Tyr294) Antibody. The picture on the right is blocked with the phospho peptide.)

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CK-1 alpha, Polyclonal Antibody (Cat# AAA316610)

Full Name

CK-1 alpha (Phospho-Tyr294) Antibody

Gene Names

CSNK1A1; CK1; CK1a; CKIa; HLCDGP1; PRO2975; HEL-S-77p

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from Jurkat cells treated with PMA 125ng/ml 30', using MAP3K7 (Phospho-Ser439) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using MAP3K7 (Phospho-Ser439) Antibody. The picture on the right is blocked with the phospho peptide.)

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MAP3K7, Polyclonal Antibody (Cat# AAA316616)

Full Name

MAP3K7 (Phospho-Ser439) Antibody

Gene Names

MAP3K7; CSCF; FMD2; TAK1; MEKK7; TGF1a

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HepG2 cells treated with serum 20% 15', using OSR1 (Phospho-Thr185) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using OSR1 (Phospho-Thr185) Antibody. The picture on the right is blocked with the phospho peptide.)

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OSR1, Polyclonal Antibody (Cat# AAA316623)

Full Name

OSR1 (Phospho-Thr185) Antibody

Gene Names

OXSR1; OSR1

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from Jurkat cells treated with starved 24h, using PKR (Phospho-Thr258) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PKR (Phospho-Thr258) Antibody. The picture on the right is blocked with the phospho peptide.)

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PKR, Polyclonal Antibody (Cat# AAA316626)

Full Name

PKR (Phospho-Thr258) Antibody

Gene Names

EIF2AK2; PKR; PRKR; EIF2AK1; PPP1R83

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HeLa cells treated with serum 20% 15', using Trk C (Phospho-Tyr516) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using Trk C (Phospho-Tyr516) Antibody. The picture on the right is blocked with the phospho peptide.)

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Trk C, Polyclonal Antibody (Cat# AAA316630)

Full Name

Trk C (Phospho-Tyr516) Antibody

Gene Names

NTRK3; TRKC; GP145-TrkC; gp145(trkC)

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from K562 cells treated with Na3VO4 0.3nM 40', using SLP-76 (Phospho-Tyr128) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using SLP-76 (Phospho-Tyr128) Antibody. The picture on the right is blocked with the phospho peptide.)

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SLP-76, Polyclonal Antibody (Cat# AAA316631)

Full Name

SLP-76 (Phospho-Tyr128) Antibody

Gene Names

LCP2; SLP76; SLP-76

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from Jurkat cells treated with TNF 20ng/ml 30' and Jurkat cells treated with UV 15', using CtBP1 (Phospho-Ser422) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using CtBP1 (Phospho-Ser422) Antibody. The picture on the right is blocked with the phospho peptide.)

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CtBP1, Polyclonal Antibody (Cat# AAA316645)

Full Name

CtBP1 (Phospho-Ser422) Antibody

Gene Names

CTBP1; BARS

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from Jurkat cells treated with IFN 2500U/ML 30', using Gab2 (Phospho-Tyr643) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using Gab2 (Phospho-Tyr643) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

Gab2, Polyclonal Antibody (Cat# AAA316647)

Full Name

Gab2 (Phospho-Tyr643) Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

< >

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WB (Western Blot) (Western blot analysis of lysates from HepG2 cells treated with PMA 125ng/ml 30', using Gab2 (Phospho-Ser623) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Gab2 (Phospho-Ser623) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

Gab2, Polyclonal Antibody (Cat# AAA316648)

Full Name

Gab2 (Phospho-Ser623) Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from RAW264.7 cells treated with UV 15', using MAPKAPK2 (Phospho-Ser272) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using MAPKAPK2 (Phospho-Ser272) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

MAPKAPK2, Polyclonal Antibody (Cat# AAA316649)

Full Name

MAPKAPK2 (Phospho-Ser272) Antibody

Gene Names

MAPKAPK2; MK2; MK-2; MAPKAP-K2

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from K562 cells treated with Na3VO4 0.3nM 40', using MAPKAPK5 (Phospho-Thr182) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MAPKAPK5 (Phospho-Thr182) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

MAPKAPK5, Polyclonal Antibody (Cat# AAA316650)

Full Name

MAPKAPK5 (Phospho-Thr182) Antibody

Gene Names

MAPKAPK5; MK5; MK-5; PRAK; MAPKAP-K5

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from HeLa cells treated with calyculinA 50ng/ml 30', using MDM4 (Phospho-Ser367) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using MDM4 (Phospho-Ser367) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

MDM4, Polyclonal Antibody (Cat# AAA316651)

Full Name

MDM4 (Phospho-Ser367) Antibody

Gene Names

MDM4; HDMX; MDMX; MRP1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from K562 cells treated with heat shock, using MKP-1/2 (Phospho-Ser296/318) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MKP-1/2 (Phospho-Ser296/318) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

MKP-1/2, Polyclonal Antibody (Cat# AAA316653)

Full Name

MKP-1/2 (Phospho-Ser296/318) Antibody

Gene Names

DUSP1; HVH1; MKP1; CL100; MKP-1; PTPN10

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from COLO205 cells treated with PMA 125ng/ml 30', using Mnk1 (Phospho-Thr385) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using Mnk1 (Phospho-Thr385) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

Mnk1, Polyclonal Antibody (Cat# AAA316654)

Full Name

Mnk1 (Phospho-Thr385) Antibody

Gene Names

MKNK1; MNK1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from rat brain, using NudC (Phospho-Ser326) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using NudC (Phospho-Ser326) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

NudC, Polyclonal Antibody (Cat# AAA316656)

Full Name

NudC (Phospho-Ser326) Antibody

Gene Names

NUDC; HNUDC; MNUDC; NPD011

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from HepG2 cells treated with TNF 20ng/ml 5', using p47 phox (Phospho-Ser345) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using p47 phox (Phospho-Ser345) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

p47 phox, Polyclonal Antibody (Cat# AAA316658)

Full Name

p47 phox (Phospho-Ser345) Antibody

Gene Names

NCF1; NCF1A; NOXO2; p47phox; SH3PXD1A

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from Jurkat cells treated with TNF 20ng/ml 30', using SREBP-1 (Phospho-Ser439) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using SREBP-1 (Phospho-Ser439) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

SREBP-1, Polyclonal Antibody (Cat# AAA316661)

Full Name

SREBP-1 (Phospho-Ser439) Antibody

Gene Names

SREBF1; SREBP1; bHLHd1

Reactivity

Human, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from RAW264.7 cells treated with TNF 20ng/ml 5', using TOP2A (Phospho-Ser1525) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using TOP2A (Phospho-Ser1525) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

TOP2A, Polyclonal Antibody (Cat# AAA316664)

Full Name

TOP2A (Phospho-Ser1525) Antibody

Gene Names

TOP2A; TOP2; TP2A

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from HeLa cells treated with IGF 100ng/ml 10' and HUVEC cells treated with EGF 200ng/ml 30', using FANCA (Phospho-Ser1149) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using FANCA (Phospho-Ser1149) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells, using FANCA (Phospho-Ser1149) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

FANCA, Polyclonal Antibody (Cat# AAA316427)

Full Name

FANCA (Phospho-Ser1149) Antibody

Gene Names

FANCA; FA; FA1; FAA; FAH; FA-H; FACA; FANCH

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from 293 cells, using SSB (Phospho-Ser366) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using SSB (Phospho-Ser366) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells treated with Forskolin 40nM 30', using SSB (Phospho-Ser366) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

SSB, Polyclonal Antibody (Cat# AAA316432)

Full Name

SSB (Phospho-Ser366) Antibody

Gene Names

SSB; La; LARP3; La/SSB

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from A549 cells treated with serum 20% 15', using HSP90B (Phospho-Ser226) Antibody. The lane on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells, using HSP90B (Phospho-Ser226) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

HSP90B, Polyclonal Antibody (Cat# AAA316437)

Full Name

HSP90B (Phospho-Ser226) Antibody

Gene Names

HSP90AB1; HSP84; HSPC2; HSPCB; D6S182; HSP90B

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from HeLa cells treated with TNF 10ng/ml 30', using KIF2C (Phospho-Ser95) Antibody. The lane on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells, using KIF2C (Phospho-Ser95) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

KIF2C, Polyclonal Antibody (Cat# AAA316439)

Full Name

KIF2C (Phospho-Ser95) Antibody

Gene Names

KIF2C; MCAK; CT139; KNSL6

Reactivity

Human, Mouse

Applications

Immunofluorescence, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from NIH/3T3 cells treated with PMA 125ng/ml 30', using Period Circadian Protein 2 (Phospho-Ser662) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using Period Circadian Protein 2 (Phospho-Ser662) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

Period Circadian Protein 2, Polyclonal Antibody (Cat# AAA316441)

Full Name

Period Circadian Protein 2 (Phospho-Ser662) Antibody

Gene Names

PER2; FASPS; FASPS1

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from COS7 cells treated with H2O2 100uM 30', using PI3-kinase p85-alpha/gamma (Phospho-Tyr467/199) Antibody. The lane on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells, using PI3-kinase p85-alpha/gamma (Phospho-Tyr467/199) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

PI3-kinase p85-alpha/gamma, Polyclonal Antibody (Cat# AAA316442)

Full Name

PI3-kinase p85-alpha/gamma (Phospho-Tyr467/199) Antibody

Gene Names

PIK3R1; p85; AGM7; GRB1; IMD36; p85-ALPHA

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from rat kidney, using PI3-kinase p85-alpha (Phospho-Tyr607) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using PI3-kinase p85-alpha (Phospho-Tyr607) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

PI3-kinase p85-alpha, Polyclonal Antibody (Cat# AAA316443)

Full Name

PI3-kinase p85-alpha (Phospho-Tyr607) Antibody

Gene Names

PIK3R1; p85; AGM7; GRB1; IMD36; p85-ALPHA

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from 293 cells, using Syntaxin 1A (Phospho-Ser14) Antibody. The lane on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells, using Syntaxin 1A (Phospho-Ser14) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

Syntaxin 1A, Polyclonal Antibody (Cat# AAA316445)

Full Name

Syntaxin 1A (Phospho-Ser14) Antibody

Gene Names

STX1A; STX1; HPC-1; P35-1; SYN1A

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

What Are Phospho Antibodies?

Protein phosphorylation is a process where a phosphate group is added to certain amino acid residues of a protein – usually serine (S), threonine (T), or tyrosine (Y) - by enzymes called kinases. This process is integral in controlling cellular signaling, cellular growth, and other biological functions.

Our catalog includes a wide range of phospho-specific antibodies that can accurately detect this important marker. They perform strongly in widely-used laboratory applications such as Western blot, flow cytometry, immunohistochemistry, and immunofluorescence microscopy. We value your trust in us and are committed to providing top-quality products and services. All of our antibodies are guaranteed to work for the applications and species indicated on our website & associated product pages.

What Are The Key Applications of Phospho Antibodies?

1. Western Blotting

One of the first steps a researcher can take in utilizing these phospho-specific antibodies, is to check if the antibody works using a technique referred to as “Western blot”. For those unfamiliar, Western Blot aids in showing whether the protein that the antibody recognizes is appearing at the correct/expected size. These phospho-specific antibodies should also be able to detect changes in the target protein’s phosphorylation (on/off state) when cells are stimulated in certain ways.

2. Staining of Fixed Cells (Immunocytochemistry)

Another routine use of these phospho-specific antibodies, is to test if the antibody is able to demonstrate similar performance when used on fixed cells (intact cells that have been preserved) as it did in the Western blot tests. It is an important aspect in many cases to confirm that the antibody works in actual intact cell samples. Ideally, the method used for cellular fixation should be the same as what is used in pathology labs (like using 10% formalin). To check if the antibody works well in tissue sections (FFPE), researchers will often test it on fixed cells that are processed similar to tissue samples.

3. Specificity Tests Using Peptides

In order to make sure that the antibody is only binding to the right target:

• Laboratory technicians will mix the antibody with phospho-peptides (short segments of the protein containing the phosphate group modification).


• If the antibody signal disappears, it is confirmation that it is binding to the correct phosphorylated location.


• A more robust test is to use both the phosphorylated and non-phosphorylated (dephosphorylated) versions of the protein. The antibody should react only with the phosphorylated one.


• Another method sometimes utilized is to treat the sample with an enzyme, such as alkaline phosphatase, that specifically removes phosphate groups. If the antibody signal disappears after this, it also confirms specificity.

4. Genetic Confirmation

As a final step, scientists can genetically manipulate the nucleotide sequence and alter the target protein by removing the exact site where phosphorylation happens. If the antibody no longer appears to detect the modified protein, it is strong evidence supporting the antibody being specific for that phosphorylated site.

Why Buy Phospho Antibodies Through Us?

• The production laboratory adheres to strict and consistent protocols prior to releasing any of these phospho-specific antibodies:


• Standard methods and proper controls in all tests to ensure high quality.


• These antibodies are tested and validated in different cell types and species.


• High quality control criterion to ensure each batch is consistent, so you will obtain reliable results every time.

FAQ

1. What Are Phospho-Specific Antibodies?

Phospho-specific antibodies are made to detect proteins only when they have a phosphate group linked to a specific amino acid residue. This empowers scientists understand if a protein is "turned on" or active, based on its phosphorylation state.

2. How to Detect Phosphorylated Proteins in a Western Blot?

To find out if a protein is phosphorylated using Western blot:

• Use a phospho-specific antibody that binds only to the phosphorylated form of the protein.


• You can also use a “regular” antibody for the same amino acid sequence of the protein that the phospho-specific antibody is binding to (but in this case, this antibody will not bind if there is a phosphate group present) in order to compare how much of it is phosphorylated versus how much is non-phosphorylated (or “total” protein, if the “normal” antibody’s epitopes are non-phospho-site-specific).

3. How to Choose the Best Antibody?

Here are some simple tips to help you pick the right antibody:

• Know your target


• Match your sample characteristics


• Confirm the intended use is appropriate


• Check “host” and “type”


• Check the “quality” of the presented data/images


• Appraise whether the available validation meets your needs