Phospho Antibodies

Phospho-specific antibodies’ typical purpose is to enable researchers to detect changes in proteins. They will exclusively bind to the amino acid sequence on a protein that has been phosphorylated (which is both a physical & chemical change) and do not bind to the same amino acid sequence on said protein if it lacks said phosphorylation. This aids in being able to clearly see and understand the data produced from this particular protein modification.

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WB (Western Blot) (Western blot analysis of lysates from Jurkat cells treated with EGF 200ng/ml 15', using PKC theta (Phospho-Ser695) Antibody.)

ELISA (Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PKC theta (Phospho-Ser695) Antibody)

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PKC theta, Polyclonal Antibody (Cat# AAA318375)

Full Name

PKC theta (Phospho-Ser695) Antibody

Gene Names

PRKCQ; PRKCT; nPKC-theta

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$345/0.05 mL $450/0.1 mL $880/2x0.1 mL $1,285/3x0.1 mL $1,660/4x0.1 mL $1,775/5x0.1 mL

WB (Western Blot) (Western blot analysis of extracts from Hela cells, untreated (-) or treated, 1:5000. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).)

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Histone H4, ELISA Kit (Cat# AAA319062)

Full Name

Histone H4 (Phospho-Ser47) Colorimetric Cell-Based ELISA Kit

Gene Names

HIST2H4B; H4/o

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of extracts from Hela cells, untreated (-) or treated, 1:5000. Secondary antibody was diluted at 1:20000)

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Histone H1, ELISA Kit (Cat# AAA319065)

Full Name

Histone H1 (Phospho-Thr17) Colorimetric Cell-Based ELISA Kit

Gene Names

HIST1H1A; H1A; H1.1; H1F1; HIST1

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of extracts from Hela cells, untreated (-) or treated, 1:5000. Secondary antibody was diluted at 1:20000)

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Histone H1, ELISA Kit (Cat# AAA319066)

Full Name

Histone H1 (Phospho-Thr3) Colorimetric Cell-Based ELISA Kit

Gene Names

HIST1H1A; H1A; H1.1; H1F1; HIST1

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of extracts from Hela cells, untreated (-) or treated, 1:2000. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).)

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Histone H3, ELISA Kit (Cat# AAA319069)

Full Name

Histone H3 (Phospho-Thr11) Colorimetric Cell-Based ELISA Kit

Gene Names

HIST1H3A; H3/A; H3FA

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of extracts from Hela cells, untreated (-) or treated, 1:2000. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).)

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Histone H3, ELISA Kit (Cat# AAA319070)

Full Name

Histone H3 (Phospho-Thr118) Colorimetric Cell-Based ELISA Kit

Gene Names

HIST1H3A; H3/A; H3FA

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of extracts from Hela cells, untreated (-) or treated, 1:2000. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).)

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Histone H3, ELISA Kit (Cat# AAA319071)

Full Name

Histone H3 (Phospho-Thr3) Colorimetric Cell-Based ELISA Kit

Gene Names

HIST1H3A; H3/A; H3FA

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4 degree C over night)

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AP2M1, ELISA Kit (Cat# AAA319083)

Full Name

AP2M1 (Phospho-Thr156) Colorimetric Cell-Based ELISA Kit

Gene Names

AP2M1; mu2; AP50; CLAPM1

Reactivity

Human, Rat, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of mouse and rat brain lysis treated or untreated by EGF 20ng/ml 30'.. Primary Antibody was diluted at 1:1000. Secondary antibody(was diluted at 1:10000)

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FoxM1, ELISA Kit (Cat# AAA319087)

Full Name

FoxM1 (Phospho-Thr600) Colorimetric Cell-Based ELISA Kit

Gene Names

FOXM1; MPP2; TGT3; HFH11; HNF-3; INS-1; MPP-2; PIG29; FKHL16; FOXM1B; HFH-11; TRIDENT; MPHOSPH2

Reactivity

Human, Rat, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4 degree C over night)

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TAZ, ELISA Kit (Cat# AAA319089)

Full Name

TAZ (Phospho-Ser89) Colorimetric Cell-Based ELISA Kit

Gene Names

WWTR1; TAZ

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4 degree C over night)

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Bim, ELISA Kit (Cat# AAA319094)

Full Name

Bim (Phospho-Ser69) Colorimetric Cell-Based ELISA Kit

Gene Names

BCL2L11; BAM; BIM; BOD

Reactivity

Human, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of various cells using Phospho-ERK 1/2 (T202/Y204) Polyclonal Antibody)

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ERK 1/2, ELISA Kit (Cat# AAA319104)

Full Name

ERK 1/2 (Phospho-Thr202/Y204) Colorimetric Cell-Based ELISA Kit

Gene Names

MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of extracts from Hela cells, 1:2000. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).)

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Histone H2A.X, ELISA Kit (Cat# AAA319107)

Full Name

Histone H2A.X (Phospho-Thr120) Colorimetric Cell-Based ELISA Kit

Gene Names

H2AFX; H2AX; H2A.X; H2A/X

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from DU145 cells, primary antibody was diluted at 1:1000, 4 degree C over night)

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Histone H2B, ELISA Kit (Cat# AAA319109)

Full Name

Histone H2B (Phospho-Ser14) Colorimetric Cell-Based ELISA Kit

Gene Names

HIST1H2BH; H2B/j; H2BFJ

Reactivity

Human, Rat, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of HELA-UV Cell Lysate using antibody)

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MDM2, ELISA Kit (Cat# AAA319113)

Full Name

MDM2 (Phospho-Tyr394) Colorimetric Cell-Based ELISA Kit

Gene Names

MDM2; HDMX; hdm2; ACTFS

Reactivity

Human, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from Jurkat cells treated with Insulin 0.01U/ml 15', using p70 S6 Kinase (Phospho-Thr389) Antibody. The lane on the right is blocked with the phospho peptide.)

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p70 S6 kinase alpha, ELISA Kit (Cat# AAA319117)

Full Name

p70 S6 kinase alpha (Phospho-Thr412) Colorimetric Cell-Based ELISA Kit

Gene Names

RPS6KB1; S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from Hela cells, treated with LPS 100ng/ml 30', using total P70 S6 Kinase Antibody and P70 S6 Kinase (phospho T389) antibody.(Green) primary antibody was diluted at 1:1000, 4 degree C over night, secondary antibody(cat:RS23920)was diluted at 1:10000, 37 degree C 1hour.)

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p70 S6 Kinase, ELISA Kit (Cat# AAA319118)

Full Name

p70 S6 Kinase (Phospho-Thr389) Colorimetric Cell-Based ELISA Kit

Gene Names

RPS6KB1; S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of various cells using Phospho-Shb (Y246) Polyclonal Antibody)

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Shb, ELISA Kit (Cat# AAA319125)

Full Name

Shb (Phospho-Tyr246) Colorimetric Cell-Based ELISA Kit

Gene Names

SHB; bA3J10.2; RP11-3J10.8

Reactivity

Human, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of extracts from K562 cells, using Phospho-Tensin-1 (Y1326) Polyclonal Antibody.)

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Tensin-1, ELISA Kit (Cat# AAA319126)

Full Name

Tensin-1 (Phospho-Tyr1326) Colorimetric Cell-Based ELISA Kit

Gene Names

TNS1; TNS; MXRA6; MST091; MST122; MST127; MSTP091; MSTP122; MSTP127; PPP1R155

Reactivity

Human, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of various cell lysis(provided by our customer). Primary Antibody was diluted at 1:1000. Secondary antibody(was diluted at 1:10000)

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ULK1, ELISA Kit (Cat# AAA319131)

Full Name

ULK1 (Phospho-Ser638) Colorimetric Cell-Based ELISA Kit

Gene Names

ULK1; ATG1; ATG1A; UNC51; hATG1; Unc51.1

Reactivity

Human, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from HCT116 cells, primary antibody was diluted at 1:1000, 4 degree C over night)

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Mcl-1, ELISA Kit (Cat# AAA319138)

Full Name

Mcl-1 (Phospho-Ser64) Colorimetric Cell-Based ELISA Kit

Gene Names

MCL1; TM; EAT; MCL1L; MCL1S; Mcl-1; BCL2L3; MCL1-ES; bcl2-L-3; mcl1/EAT

Reactivity

Human, Rat, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of 3T3 cells using Phospho-Ron (S1394) Polyclonal Antibody diluted at 1:500)

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Ron, ELISA Kit (Cat# AAA319146)

Full Name

Ron (Phospho-Ser1394) Colorimetric Cell-Based ELISA Kit

Gene Names

MST1R; RON; PTK8; CD136; CDw136

Reactivity

Human, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from SW480 cells, primary antibody was diluted at 1:1000, 4 degree C over night)

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PZR, ELISA Kit (Cat# AAA319153)

Full Name

PZR (Phospho-Tyr241) Colorimetric Cell-Based ELISA Kit

Gene Names

MPZL1; PZR; PZRa; PZRb; PZR1b; MPZL1b

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of various cells using Phospho-Akt1 (S129) Polyclonal Antibody diluted at 1:1000)

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Akt1, ELISA Kit (Cat# AAA319156)

Full Name

Akt1 (Phospho-Ser129) Colorimetric Cell-Based ELISA Kit

Gene Names

AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of MOUSE-HEART cells using Phospho-Bcl-6 (S333) Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).)

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Bcl-6, ELISA Kit (Cat# AAA319160)

Full Name

Bcl-6 (Phospho-Ser333) Colorimetric Cell-Based ELISA Kit

Gene Names

BCL6; BCL5; LAZ3; BCL6A; ZNF51; ZBTB27

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of 293 cells using Phospho-Ezrin (Y146) Polyclonal Antibody)

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Ezrin, ELISA Kit (Cat# AAA319169)

Full Name

Ezrin (Phospho-Tyr146) Colorimetric Cell-Based ELISA Kit

Gene Names

EZR; CVL; CVIL; VIL2

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from Jurkat cells treated with starved 24h, using Kv1.3/KCNA3 (Phospho-Tyr135) Antibody. The lane on the right is blocked with the phospho peptide.)

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Kv1.3, ELISA Kit (Cat# AAA319176)

Full Name

Kv1.3 (Phospho-Tyr187) Colorimetric Cell-Based ELISA Kit

Gene Names

KCNA3; MK3; HGK5; HLK3; PCN3; HPCN3; KV1.3; HUKIII

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of various cells using Phospho-LIMK-1/2 (T508/505) Polyclonal Antibody)

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LIMK-1/2, ELISA Kit (Cat# AAA319177)

Full Name

LIMK-1/2 (Phospho-Thr508/505) Colorimetric Cell-Based ELISA Kit

Gene Names

LIMK1; LIMK; LIMK-1

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of LIMK2 (Phospho-Ser283) Antibody. The lane on the right is blocked with the LIMK2 (Phospho-Ser283) peptide.)

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LIMK-2, ELISA Kit (Cat# AAA319178)

Full Name

LIMK-2 (Phospho-Ser283) Colorimetric Cell-Based ELISA Kit

Reactivity

Human, Mouse, Rat, Monkey

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of K562 using p-Mcl-1 (S159) antibody.)

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Mcl-1, ELISA Kit (Cat# AAA319179)

Full Name

Mcl-1 (Phospho-Ser159) Colorimetric Cell-Based ELISA Kit

Gene Names

MCL1; TM; EAT; MCL1L; MCL1S; Mcl-1; BCL2L3; MCL1-ES; bcl2-L-3; mcl1/EAT

Reactivity

Human, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of various cells using Phospho-MOR-1 (S375) Polyclonal Antibody)

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MOR-1, ELISA Kit (Cat# AAA319180)

Full Name

MOR-1 (Phospho-Ser375) Colorimetric Cell-Based ELISA Kit

Gene Names

OPRM1; MOP; MOR; LMOR; MOR1; OPRM; M-OR-1

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from Jurkat cells treated with PMA 125ng/ml 30' and Jurkat cells treated with insulin 0.01U/ml 15', using Retinoic Acid Receptor alpha (Phospho-Ser77) Antibody. The lane on the right is blocked with the phospho peptide.)

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RARalpha, ELISA Kit (Cat# AAA319193)

Full Name

RARalpha (Phospho-Ser77) Colorimetric Cell-Based ELISA Kit

Gene Names

RARA; RAR; NR1B1

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of K562 cells using Phospho-SLP-76 (Y128) Polyclonal Antibody)

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SLP-76, ELISA Kit (Cat# AAA319194)

Full Name

SLP-76 (Phospho-Tyr128) Colorimetric Cell-Based ELISA Kit

Gene Names

LCP2; SLP76; SLP-76

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysate from Jurkat cells, using phospho-Vimentin (Phospho-Tyr61) antibody.)

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Vimentin, ELISA Kit (Cat# AAA319202)

Full Name

Vimentin (Phospho-Tyr61) Colorimetric Cell-Based ELISA Kit

Reactivity

Human, Rat, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from K562 cells, using AurB (Phospho-Tyr12) Antibody. The lane on the right is blocked with the phospho peptide.)

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ARK-2, ELISA Kit (Cat# AAA319210)

Full Name

ARK-2 (Phospho-Tyr12) Colorimetric Cell-Based ELISA Kit

Gene Names

AURKB; AIK2; AIM1; ARK2; AurB; IPL1; STK5; AIM-1; STK12; PPP1R48; aurkb-sv1; aurkb-sv2

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of K562 using p-Atm (S1981) antibody. Antibody was diluted at 1:500)

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Atm, ELISA Kit (Cat# AAA319212)

Full Name

Atm (Phospho-Ser1981) Colorimetric Cell-Based ELISA Kit

Gene Names

ATM; AT1; ATA; ATC; ATD; ATE; ATDC; TEL1; TELO1

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from COLO205 cells treated with EGF 200ng/ml 30', using Cyclin D2 (Phospho-Thr280) Antibody. The lane on the right is blocked with the phospho peptide.)

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Cyclin D2, ELISA Kit (Cat# AAA319221)

Full Name

Cyclin D2 (Phospho-Thr280) Colorimetric Cell-Based ELISA Kit

Gene Names

CCND2; KIAK0002

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of DDX3/DEAD-box Protein 3 (Phospho-Thr322) Antibody. The lane on the right is blocked with the DDX3/DEAD-box Protein 3 (Phospho-Thr322) peptide.)

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DDX3, ELISA Kit (Cat# AAA319223)

Full Name

DDX3 (Phospho-Thr322) Colorimetric Cell-Based ELISA Kit

Gene Names

DDX3X; DBX; DDX3; HLP2; DDX14

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4 degree C over night)

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Doublecortin, ELISA Kit (Cat# AAA319224)

Full Name

Doublecortin (Phospho-Ser378) Colorimetric Cell-Based ELISA Kit

Gene Names

DCX; DC; DBCN; LISX; SCLH; XLIS

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of various cells using Phospho-Filamin 1 (S2152) Polyclonal Antibody diluted at 1:2000)

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Filamin 1, ELISA Kit (Cat# AAA319229)

Full Name

Filamin 1 (Phospho-Ser2152) Colorimetric Cell-Based ELISA Kit

Gene Names

FLNA; FLN; FMD; MNS; OPD; ABPX; CSBS; CVD1; FLN1; NHBP; OPD1; OPD2; XLVD; XMVD; FLN-A; ABP-280

Reactivity

Human, Mouse, Rat, Monkey

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of various cells using Phospho-IRS-1 (S639) Polyclonal Antibody diluted at 1:500)

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IRS-1, ELISA Kit (Cat# AAA319243)

Full Name

IRS-1 (Phospho-Ser639) Colorimetric Cell-Based ELISA Kit

Gene Names

IRS1; HIRS-1

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of various cells using Phospho-JNK1/2/3 (Y185) Polyclonal Antibody)

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JNK1/2/3, ELISA Kit (Cat# AAA319246)

Full Name

JNK1/2/3 (Phospho-Tyr185) Colorimetric Cell-Based ELISA Kit

Gene Names

MAPK8; JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of 293 cells using Phospho-KIF20A (S528) Polyclonal Antibody)

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KIF20A, ELISA Kit (Cat# AAA319247)

Full Name

KIF20A (Phospho-Ser528) Colorimetric Cell-Based ELISA Kit

Gene Names

KIF20A; MKLP2; RAB6KIFL

Reactivity

Human, Rat, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of various cells using Phospho-KOR-1 (S369) Polyclonal Antibody diluted at 1:1000)

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KOR-1, ELISA Kit (Cat# AAA319249)

Full Name

KOR-1 (Phospho-Ser369) Colorimetric Cell-Based ELISA Kit

Gene Names

Oprk1; KOR; R21; KOR-1; MSL-1; Oprk2; K-OR-1

Reactivity

Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of COS cell lysis treated by UV. Primary Antibody was diluted at 1:1000. Secondary antibody(was diluted at 1:10000)

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LKB1, ELISA Kit (Cat# AAA319251)

Full Name

LKB1 (Phospho-Ser334) Colorimetric Cell-Based ELISA Kit

Gene Names

STK11; PJS; LKB1; hLKB1

Reactivity

Human, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from HepG2 cells treated with PMA 125ng/ml 30', using MAK (Phospho-Tyr159) Antibody. The lane on the right is blocked with the phospho peptide.)

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MAK, ELISA Kit (Cat# AAA319252)

Full Name

MAK (Phospho-Tyr159) Colorimetric Cell-Based ELISA Kit

Gene Names

MAK; RP62; dJ417M14.2

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of K562 cells, cell treated or untreated by EGF 20ng/ml 30'. Primary Antibody was diluted at 1:1000. Secondary antibody(was diluted at 1:10000)

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eNOS, ELISA Kit (Cat# AAA319265)

Full Name

eNOS (Phospho-Ser1179) Colorimetric Cell-Based ELISA Kit

Gene Names

NOS3; eNOS; ECNOS

Reactivity

Human, Mouse, Rat

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from LOVO cells treated with PMA 125ng/ml 30', using PAK5/6 (Phospho-Ser602/Ser560) Antibody. The lane on the right is blocked with the phospho peptide.)

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PAK5/6, ELISA Kit (Cat# AAA319269)

Full Name

PAK5/6 (Phospho-Ser602/S560) Colorimetric Cell-Based ELISA Kit

Gene Names

PAK7; PAK5

Reactivity

Human, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western blot analysis of lysates from LOVO cells treated with starved 24h, using PAKalpha (Phospho-Ser199) Antibody. The lane on the right is blocked with the phospho peptide.)

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PAKalpha, ELISA Kit (Cat# AAA319270)

Full Name

PAKalpha (Phospho-Ser199) Colorimetric Cell-Based ELISA Kit

Gene Names

PAK1; PAKalpha

Reactivity

Human, Mouse, Rat, Monkey

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

WB (Western Blot) (Western Blot analysis of various cells using Phospho-Paxillin (Y88) Polyclonal Antibody)

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Paxillin, ELISA Kit (Cat# AAA319271)

Full Name

Paxillin (Phospho-Tyr88) Colorimetric Cell-Based ELISA Kit

Reactivity

Human, Rat, Mouse

Pricing

$590/1 Kit $1,140/2x1 Kit $1,665/3x1 Kit $2,160/4x1 Kit $2,690/5x1 Kit

What Are Phospho Antibodies?

Protein phosphorylation is a process where a phosphate group is added to certain amino acid residues of a protein – usually serine (S), threonine (T), or tyrosine (Y) - by enzymes called kinases. This process is integral in controlling cellular signaling, cellular growth, and other biological functions.

Our catalog includes a wide range of phospho-specific antibodies that can accurately detect this important marker. They perform strongly in widely-used laboratory applications such as Western blot, flow cytometry, immunohistochemistry, and immunofluorescence microscopy. We value your trust in us and are committed to providing top-quality products and services. All of our antibodies are guaranteed to work for the applications and species indicated on our website & associated product pages.

What Are The Key Applications of Phospho Antibodies?

1. Western Blotting

One of the first steps a researcher can take in utilizing these phospho-specific antibodies, is to check if the antibody works using a technique referred to as “Western blot”. For those unfamiliar, Western Blot aids in showing whether the protein that the antibody recognizes is appearing at the correct/expected size. These phospho-specific antibodies should also be able to detect changes in the target protein’s phosphorylation (on/off state) when cells are stimulated in certain ways.

2. Staining of Fixed Cells (Immunocytochemistry)

Another routine use of these phospho-specific antibodies, is to test if the antibody is able to demonstrate similar performance when used on fixed cells (intact cells that have been preserved) as it did in the Western blot tests. It is an important aspect in many cases to confirm that the antibody works in actual intact cell samples. Ideally, the method used for cellular fixation should be the same as what is used in pathology labs (like using 10% formalin). To check if the antibody works well in tissue sections (FFPE), researchers will often test it on fixed cells that are processed similar to tissue samples.

3. Specificity Tests Using Peptides

In order to make sure that the antibody is only binding to the right target:

• Laboratory technicians will mix the antibody with phospho-peptides (short segments of the protein containing the phosphate group modification).


• If the antibody signal disappears, it is confirmation that it is binding to the correct phosphorylated location.


• A more robust test is to use both the phosphorylated and non-phosphorylated (dephosphorylated) versions of the protein. The antibody should react only with the phosphorylated one.


• Another method sometimes utilized is to treat the sample with an enzyme, such as alkaline phosphatase, that specifically removes phosphate groups. If the antibody signal disappears after this, it also confirms specificity.

4. Genetic Confirmation

As a final step, scientists can genetically manipulate the nucleotide sequence and alter the target protein by removing the exact site where phosphorylation happens. If the antibody no longer appears to detect the modified protein, it is strong evidence supporting the antibody being specific for that phosphorylated site.

Why Buy Phospho Antibodies Through Us?

• The production laboratory adheres to strict and consistent protocols prior to releasing any of these phospho-specific antibodies:


• Standard methods and proper controls in all tests to ensure high quality.


• These antibodies are tested and validated in different cell types and species.


• High quality control criterion to ensure each batch is consistent, so you will obtain reliable results every time.

FAQ

1. What Are Phospho-Specific Antibodies?

Phospho-specific antibodies are made to detect proteins only when they have a phosphate group linked to a specific amino acid residue. This empowers scientists understand if a protein is "turned on" or active, based on its phosphorylation state.

2. How to Detect Phosphorylated Proteins in a Western Blot?

To find out if a protein is phosphorylated using Western blot:

• Use a phospho-specific antibody that binds only to the phosphorylated form of the protein.


• You can also use a “regular” antibody for the same amino acid sequence of the protein that the phospho-specific antibody is binding to (but in this case, this antibody will not bind if there is a phosphate group present) in order to compare how much of it is phosphorylated versus how much is non-phosphorylated (or “total” protein, if the “normal” antibody’s epitopes are non-phospho-site-specific).

3. How to Choose the Best Antibody?

Here are some simple tips to help you pick the right antibody:

• Know your target


• Match your sample characteristics


• Confirm the intended use is appropriate


• Check “host” and “type”


• Check the “quality” of the presented data/images


• Appraise whether the available validation meets your needs