Phospho Antibodies

Phospho-specific antibodies’ typical purpose is to enable researchers to detect changes in proteins. They will exclusively bind to the amino acid sequence on a protein that has been phosphorylated (which is both a physical & chemical change) and do not bind to the same amino acid sequence on said protein if it lacks said phosphorylation. This aids in being able to clearly see and understand the data produced from this particular protein modification.

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WB (Western Blot) (Western blot analysis of lysates from NIH/3T3 cells treated with PDGF 50ng/ml 20', using Akt1 (Phospho-Thr450) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using Akt1 (Phospho-Thr450) Antibody. The picture on the right is blocked with the phospho peptide.)

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Akt1, Polyclonal Antibody (Cat# AAA316128)

Full Name

Akt1 (Phospho-Thr450) Antibody

Gene Names

AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from Jurkat cells, using AML1 (Phospho-Ser303) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using AML1 (Phospho-Ser303) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

AML1, Polyclonal Antibody (Cat# AAA316130)

Full Name

AML1 (Phospho-Ser303) Antibody

Gene Names

RUNX1; AML1; CBFA2; EVI-1; AMLCR1; PEBP2aB; CBF2alpha; AML1-EVI-1; PEBP2alpha

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from COLO205 cells and HUVEC cells, using AMPK beta1 (Phospho-Ser181) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human skeletal muscle, using AMPK beta1 (Phospho-Ser181) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

AMPK beta1, Polyclonal Antibody (Cat# AAA316131)

Full Name

AMPK beta1 (Phospho-Ser181) Antibody

Gene Names

PRKAB1; AMPK; HAMPKb

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from K562 cells treated with forskolin 40nM 30', using Fos (Phospho-Ser362) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human thyroid gland, using Fos (Phospho-Ser362) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

Fos, Polyclonal Antibody (Cat# AAA316141)

Full Name

Fos (Phospho-Ser362) Antibody

Gene Names

FOS; p55; AP-1; C-FOS

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from NIH/3T3 cells treated with PMA 125ng/ml 30', using Glycogen Synthase (Phospho-Ser645) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human skeletal muscle, using Glycogen Synthase (Phospho-Ser645) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

Glycogen Synthase, Polyclonal Antibody (Cat# AAA316143)

Full Name

Glycogen Synthase (Phospho-Ser645) Antibody

Gene Names

GYS1; GSY; GYS

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from Jurkat cells treated with EGF 200ng/ml 15', using GR (Phospho-Ser226) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using GR (Phospho-Ser226) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

GR, Polyclonal Antibody (Cat# AAA316144)

Full Name

GR (Phospho-Ser226) Antibody

Gene Names

NR3C1; GR; GCR; GRL; GCCR; GCRST

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HepG2 cells treated with TNF 20ng/ml 5', using IKK-alpha/beta (Phospho-Ser180/181) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human heart, using IKK-alpha/beta (Phospho-Ser180/181) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

IKK-alpha/beta, Polyclonal Antibody (Cat# AAA316148)

Full Name

IKK-alpha/beta (Phospho-Ser180/181) Antibody

Gene Names

CHUK; IKK1; IKKA; IKBKA; TCF16; NFKBIKA; IKK-alpha

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HeLa cells, using Ku70 (Phospho-Ser5) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using Ku70 (Phospho-Ser5) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

Ku70, Polyclonal Antibody (Cat# AAA316156)

Full Name

Ku70 (Phospho-Ser5) Antibody

Gene Names

XRCC6; ML8; KU70; TLAA; CTC75; CTCBF; G22P1

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from COS7 cells treated with Etoposide 25uM 60', using Arrestin 1 (Phospho-Ser412) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Arrestin 1 (Phospho-Ser412) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

Arrestin 1, Polyclonal Antibody (Cat# AAA316158)

Full Name

Arrestin 1 (Phospho-Ser412) Antibody

Gene Names

ARRB1; ARB1; ARR1

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using AS160 (Phospho-Thr642) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells, using AS160 (Phospho-Thr642) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

AS160, Polyclonal Antibody (Cat# AAA316160)

Full Name

AS160 (Phospho-Thr642) Antibody

Gene Names

TBC1D4; AS160; NIDDM5

Reactivity

Human, Mouse

Applications

Immunofluorescence, Immunohistochemistry

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from 293 cells treated with serum 20% 15', using Aurora Kinase (Phospho-Thr288) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Aurora Kinase (Phospho-Thr288) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

Aurora Kinase, Polyclonal Antibody (Cat# AAA316162)

Full Name

Aurora Kinase (Phospho-Thr288) Antibody

Gene Names

AURKA; AIK; ARK1; AURA; BTAK; STK6; STK7; STK15; PPP1R47

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HeLa cells treated with EGF 200ng/ml 30', using Calnexin (Phospho-Ser583) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Calnexin (Phospho-Ser583) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells, using Calnexin (Phospho-Ser583) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

Calnexin, Polyclonal Antibody (Cat# AAA316165)

Full Name

Calnexin (Phospho-Ser583) Antibody

Gene Names

CANX; CNX; P90; IP90

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from 293 cells treated with Serum 20% 15', using FOXO1/3/4-pan (Phospho-Thr24/32) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using FOXO1/3/4-pan (Phospho-Thr24/32) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

FOXO1/3/4-pan, Polyclonal Antibody (Cat# AAA316177)

Full Name

FOXO1/3/4-pan (Phospho-Thr24/32) Antibody

Gene Names

FOXO1; FKH1; FKHR; FOXO1A

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HeLa cells treated with Serum 20% 15', using FOXO1A (Phospho-Ser329) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using FOXO1A (Phospho-Ser329) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

FOXO1A, Polyclonal Antibody (Cat# AAA316178)

Full Name

FOXO1A (Phospho-Ser329) Antibody

Gene Names

FOXO1; FKH1; FKHR; FOXO1A

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from 293 cells treated with Heat shock, using IR (Phospho-Tyr1355) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using IR (Phospho-Tyr1355) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

IR, Polyclonal Antibody (Cat# AAA316183)

Full Name

IR (Phospho-Tyr1355) Antibody

Gene Names

INSR; HHF5; CD220

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HT29 cells treated with INSULIN 0.01U/ML 15', using IRF-3 (Phospho-Ser385) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using IRF-3 (Phospho-Ser385) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells, using IRF-3 (Phospho-Ser385) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

IRF-3, Polyclonal Antibody (Cat# AAA316185)

Full Name

IRF-3 (Phospho-Ser385) Antibody

Gene Names

IRF3; IIAE7

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from NIH/3T3 cells, using MYPT1 (Phospho-Thr853) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using MYPT1 (Phospho-Thr853) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

MYPT1, Polyclonal Antibody (Cat# AAA316196)

Full Name

MYPT1 (Phospho-Thr853) Antibody

Gene Names

PPP1R12A; MBS; M130; MYPT1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HeLa cells treated with Ca+ 40nM 30', using NFAT4 (Phospho-Ser165) Antibody. The lane on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells, using NFAT4 (Phospho-Ser165) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

NFAT4, Polyclonal Antibody (Cat# AAA316197)

Full Name

NFAT4 (Phospho-Ser165) Antibody

Gene Names

NFATC3; NFAT4; NFATX

Reactivity

Human, Mouse

Applications

Immunofluorescence, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from COLO205 cells treated with serum 20% 15', using p70 S6 Kinase (Phospho-Ser371) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using p70 S6 Kinase (Phospho-Ser371) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

p70 S6 Kinase, Polyclonal Antibody (Cat# AAA316202)

Full Name

p70 S6 Kinase (Phospho-Ser371) Antibody

Gene Names

RPS6KB1; S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HT29 cells treated with serum 20% 15', using p70 S6 Kinase (Phospho-Ser418) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using p70 S6 Kinase (Phospho-Ser418) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of COS7 cells, using p70 S6 Kinase (Phospho-Ser418) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

p70 S6 Kinase, Polyclonal Antibody (Cat# AAA316204)

Full Name

p70 S6 Kinase (Phospho-Ser418) Antibody

Gene Names

RPS6KB1; S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from 293 cells treated with etoposide 25uM 1h, using PAK1 (Phospho-Thr212) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PAK1 (Phospho-Thr212) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

PAK1, Polyclonal Antibody (Cat# AAA316207)

Full Name

PAK1 (Phospho-Thr212) Antibody

Gene Names

PAK1; PAKalpha

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from COS7 cells treated with TNF 20ng/ml 5', using GRK1 (Phospho-Ser21) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using GRK1 (Phospho-Ser21) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

GRK1, Polyclonal Antibody (Cat# AAA316366)

Full Name

GRK1 (Phospho-Ser21) Antibody

Gene Names

GRK1; RK; RHOK; GPRK1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from HUVEC cells treated with EGF 200ng/ml 30', using HER3 (Phospho-Tyr1222) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using HER3 (Phospho-Tyr1222) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HUVEC cells, using HER3 (Phospho-Tyr1222) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

HER3, Polyclonal Antibody (Cat# AAA316369)

Full Name

HER3 (Phospho-Tyr1222) Antibody

Gene Names

ERBB3; HER3; LCCS2; ErbB-3; c-erbB3; erbB3-S; MDA-BF-1; c-erbB-3; p180-ErbB3; p45-sErbB3; p85-sErbB3

Reactivity

Human, Rat, Mouse

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using NMDAR2A/B (Phospho-Tyr1246/1252) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HUVEC cells, using NMDAR2A/B (Phospho-Tyr1246/1252) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

NMDAR2A/B, Polyclonal Antibody (Cat# AAA316374)

Full Name

NMDAR2A/B (Phospho-Tyr1246/1252) Antibody

Gene Names

GRIN2A; LKS; EPND; FESD; NR2A; GluN2A; NMDAR2A

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from 293 cells and HepG2 cells, using Smad1 (Phospho-Ser187) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Smad1 (Phospho-Ser187) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

Smad1, Polyclonal Antibody (Cat# AAA316380)

Full Name

Smad1 (Phospho-Ser187) Antibody

Gene Names

SMAD1; BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using Synapsin1 (Phospho-Ser605) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of COS7 cells, using Synapsin1 (Phospho-Ser605) Antibody. The picture on the right is blocked with the phospho peptide.)

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Synapsin1, Polyclonal Antibody (Cat# AAA316384)

Full Name

Synapsin1 (Phospho-Ser605) Antibody

Gene Names

SYN1; SYNI; SYN1a; SYN1b

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Vinculin (Phospho-Tyr821) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HepG2 cells, using Vinculin (Phospho-Tyr821) Antibody. The picture on the right is blocked with the phospho peptide.)

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Vinculin, Polyclonal Antibody (Cat# AAA316387)

Full Name

Vinculin (Phospho-Tyr821) Antibody

Gene Names

VCL; MV; MVCL; CMD1W; CMH15; HEL114

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using APC1 (Phospho-Ser688) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of COS7 cells, using APC1 (Phospho-Ser688) Antibody. The picture on the right is blocked with the phospho peptide.)

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APC1, Polyclonal Antibody (Cat# AAA316388)

Full Name

APC1 (Phospho-Ser688) Antibody

Gene Names

ANAPC1; APC1; MCPR; TSG24

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HUVEC cells, using CDC16/APC6 (Phospho-Ser560) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using CDC16/APC6 (Phospho-Ser560) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells, using CDC16/APC6 (Phospho-Ser560) Antibody. The picture on the right is blocked with the phospho peptide.)

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CDC16/APC6, Polyclonal Antibody (Cat# AAA316392)

Full Name

CDC16/APC6 (Phospho-Ser560) Antibody

Gene Names

CDC16; APC6; CUT9; ANAPC6; CDC16Hs

Reactivity

Human, Mouse

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from NIH/3T3 cells treated with Insulin 0.01U/ml 15', using GRB10 (Phospho-Tyr67) Antibody. The lane on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HepG2 cells, using GRB10 (Phospho-Tyr67) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

GRB10, Polyclonal Antibody (Cat# AAA316394)

Full Name

GRB10 (Phospho-Tyr67) Antibody

Gene Names

GRB10; RSS; IRBP; MEG1; GRB-IR; Grb-10

Reactivity

Human

Applications

Immunofluorescence, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from COS7 cells, using GTPase Activating Protein (Phospho-Ser387) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human placenta, using GTPase Activating Protein (Phospho-Ser387) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells, using GTPase Activating Protein (Phospho-Ser387) Antibody. The picture on the right is blocked with the phospho peptide.)

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GTPase Activating Protein, Polyclonal Antibody (Cat# AAA316395)

Full Name

GTPase Activating Protein (Phospho-Ser387) Antibody

Gene Names

RACGAP1; CYK4; ID-GAP; HsCYK-4; MgcRacGAP

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HUVEC and 293 cells, using hnRPD (Phospho-Ser83) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using hnRPD (Phospho-Ser83) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells, using hnRPD (Phospho-Ser83) Antibody. The picture on the right is blocked with the phospho peptide.)

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hnRPD, Polyclonal Antibody (Cat# AAA316398)

Full Name

hnRPD (Phospho-Ser83) Antibody

Gene Names

HNRNPD; P37; AUF1; AUF1A; HNRPD; hnRNPD0

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from COS7 cells treated with nocodazole 1ug/ml 16h, using IkappaB-alpha (Phospho-Tyr305) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human lymph node, using IkappaB-alpha (Phospho-Tyr305) Antibody. The picture on the right is blocked with the phospho peptide.)

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IkappaB-alpha, Polyclonal Antibody (Cat# AAA316401)

Full Name

IkappaB-alpha (Phospho-Tyr305) Antibody

Gene Names

NFKBIA; IKBA; MAD-3; NFKBI

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from HUVEC cells treated with serum 20% 15', using IL-13R alpha1 (Phospho-Tyr405) Antibody. The lane on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HepG2 cells, using IL-13R alpha1 (Phospho-Tyr405) Antibody. The picture on the right is blocked with the phospho peptide.)

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IL-13R alpha1, Polyclonal Antibody (Cat# AAA316402)

Full Name

IL-13R alpha1 (Phospho-Tyr405) Antibody

Gene Names

IL13RA1; NR4; CT19; CD213A1; IL-13Ra

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from Jurkat cells treated with heat shock, using IRAK1 (Phospho-Thr100) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using IRAK1 (Phospho-Thr100) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

IRAK1, Polyclonal Antibody (Cat# AAA316409)

Full Name

IRAK1 (Phospho-Thr100) Antibody

Gene Names

IRAK1; IRAK; pelle

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from 293 cells treated with insulin 0.01U/ml 30', using IREB1 (Phospho-Ser711) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human thyroid gland, using IREB1 (Phospho-Ser711) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

IREB1, Polyclonal Antibody (Cat# AAA316410)

Full Name

IREB1 (Phospho-Ser711) Antibody

Gene Names

ACO1; IRP1; ACONS; HEL60; IREB1; IREBP; IREBP1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from HeLa cells, using Kir6.2 (Phospho-Thr224) Antibody. The lane on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HUVEC cells, using Kir6.2 (Phospho-Thr224) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

Kir6.2, Polyclonal Antibody (Cat# AAA316414)

Full Name

Kir6.2 (Phospho-Thr224) Antibody

Gene Names

KCNJ11; BIR; HHF2; PHHI; IKATP; TNDM3; KIR6.2; MODY13

Reactivity

Human, Mouse

Applications

Immunofluorescence, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using Kv2.1/KCNB1 (Phospho-Ser567) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HepG2 cells, using Kv2.1/KCNB1 (Phospho-Ser567) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

Kv2.1/KCNB1, Polyclonal Antibody (Cat# AAA316416)

Full Name

Kv2.1/KCNB1 (Phospho-Ser567) Antibody

Gene Names

KCNB1; DRK1; Kv2.1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from COS7 cells treated with H2O2 100uM 30', using RAD52 (Phospho-Tyr104) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human testis, using RAD52 (Phospho-Tyr104) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

RAD52, Polyclonal Antibody (Cat# AAA316421)

Full Name

RAD52 (Phospho-Tyr104) Antibody

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from HepG2 cells treated with nocodazole 1ug/ml 16h, using Adrenergic Receptor beta2 (Phospho-Ser346) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using Adrenergic Receptor beta2 (Phospho-Ser346) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells, using Adrenergic Receptor beta2 (Phospho-Ser346) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

Adrenergic Receptor beta2, Polyclonal Antibody (Cat# AAA316424)

Full Name

Adrenergic Receptor beta2 (Phospho-Ser346) Antibody

Gene Names

ADRB2; BAR; B2AR; ADRBR; ADRB2R; BETA2AR

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

Application Data (The TFact c-Jun DNA-Binding ELISA kits detect active c-Jun in Hela Nuclear Extract. The Hela cells were grown 3 days in DMEM with 10% FBS and harvested for nuclear extract. The Hela cells were stimulated by UV (100J/M2) before harvest.)

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c-Jun, ELISA Kit (Cat# AAA315851)

Full Name

c-Jun (Phospho-Ser73) TFact DNA-Binding ELISA Kit

Gene Names

JUN; AP1; p39; AP-1; c-Jun

Reactivity

Human

Pricing

$635/1 Kit $1,230/2x1 Kit $1,800/3x1 Kit $2,335/4x1 Kit $2,905/5x1 Kit

Application Data (The TFact ATF2 (Phospho-Thr71 or 53) DNA-Binding ELISA detects active ATF2 (Phospho-Thr71 or 53) in Hela Nuclear Extract. The Hela cells were grown 3 days in DMEM with 10% FBS and harvested for nuclear extract. The Hela cells were stimulated by UV (100J/M2) before harvest.)

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ATF2, ELISA Kit (Cat# AAA315869)

Full Name

ATF2 (Phospho-Thr71 or 53) TFact DNA-Binding ELISA Kit

Gene Names

ATF2; HB16; CREB2; TREB7; CREB-2; CRE-BP1

Reactivity

Human, Mouse, Rat

Pricing

$635/1 Kit $1,230/2x1 Kit $1,800/3x1 Kit $2,335/4x1 Kit $2,905/5x1 Kit

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<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from 293 cells treated with PMA 125ng/ml 30', using CD5 (Phospho-Tyr453) Antibody. The lane on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HepG2 cells, using CD5 (Phospho-Tyr453) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

CD5, Polyclonal Antibody (Cat# AAA316330)

Full Name

CD5 (Phospho-Tyr453) Antibody

Gene Names

CD5; T1; LEU1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from 293 cells treated with PMA 125ng/ml 30', using CD71/TfR (Phospho-Ser24) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using CD71/TfR (Phospho-Ser24) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

CD71/TfR, Polyclonal Antibody (Cat# AAA316331)

Full Name

CD71/TfR (Phospho-Ser24) Antibody

Gene Names

TFRC; T9; TR; TFR; p90; CD71; TFR1; TRFR; IMD46

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from K562 cells treated with Na3VO4 0.3uM 40', using Chk1 (Phospho-Ser286) Antibody. The lane on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells, using Chk1 (Phospho-Ser286) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

Chk1, Polyclonal Antibody (Cat# AAA316334)

Full Name

Chk1 (Phospho-Ser286) Antibody

Gene Names

CHEK1; CHK1

Reactivity

Human

Applications

Immunofluorescence, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from 293 cells, using Chk1 (Phospho-Ser301) Antibody. The lane on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells, using Chk1 (Phospho-Ser301) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

Chk1, Polyclonal Antibody (Cat# AAA316335)

Full Name

Chk1 (Phospho-Ser301) Antibody

Gene Names

CHEK1; CHK1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human testis, using c-Jun (Phospho-Thr231) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells, using c-Jun (Phospho-Thr231) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

c-Jun, Polyclonal Antibody (Cat# AAA316337)

Full Name

c-Jun (Phospho-Thr231) Antibody

Gene Names

JUN; AP1; p39; AP-1; c-Jun

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from RAW264.7 cells treated with TNF 20ng/ml 30', using Coagulation Factor III (Phospho-Ser290) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using Coagulation Factor III (Phospho-Ser290) Antibody. The picture on the right is blocked with the phospho peptide.)

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Coagulation Factor III, Polyclonal Antibody (Cat# AAA316340)

Full Name

Coagulation Factor III (Phospho-Ser290) Antibody

Gene Names

F3; TF; TFA; CD142

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from HUVEC cells, using Catenin-delta1 (Phospho-Tyr228) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using Catenin-delta1 (Phospho-Tyr228) Antibody. The picture on the right is blocked with the phospho peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HUVEC cells, using Catenin-delta1 (Phospho-Tyr228) Antibody. The picture on the right is blocked with the phospho peptide.)

Compare (5 max)

Catenin-delta1, Polyclonal Antibody (Cat# AAA316346)

Full Name

Catenin-delta1 (Phospho-Tyr228) Antibody

Gene Names

CTNND1; CAS; p120; BCDS2; CTNND; P120CAS; P120CTN; p120(CAS); p120(CTN)

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from COS7 cells treated with anisomycin 25ug/ml 30', using CRYAB (Phospho-Ser45) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human heart, using CRYAB (Phospho-Ser45) Antibody. The picture on the right is blocked with the phospho peptide.)

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CRYAB, Polyclonal Antibody (Cat# AAA316348)

Full Name

CRYAB (Phospho-Ser45) Antibody

Gene Names

CRYAB; MFM2; CRYA2; CTPP2; HSPB5; CMD1II; CTRCT16; HEL-S-101

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

What Are Phospho Antibodies?

Protein phosphorylation is a process where a phosphate group is added to certain amino acid residues of a protein – usually serine (S), threonine (T), or tyrosine (Y) - by enzymes called kinases. This process is integral in controlling cellular signaling, cellular growth, and other biological functions.

Our catalog includes a wide range of phospho-specific antibodies that can accurately detect this important marker. They perform strongly in widely-used laboratory applications such as Western blot, flow cytometry, immunohistochemistry, and immunofluorescence microscopy. We value your trust in us and are committed to providing top-quality products and services. All of our antibodies are guaranteed to work for the applications and species indicated on our website & associated product pages.

What Are The Key Applications of Phospho Antibodies?

1. Western Blotting

One of the first steps a researcher can take in utilizing these phospho-specific antibodies, is to check if the antibody works using a technique referred to as “Western blot”. For those unfamiliar, Western Blot aids in showing whether the protein that the antibody recognizes is appearing at the correct/expected size. These phospho-specific antibodies should also be able to detect changes in the target protein’s phosphorylation (on/off state) when cells are stimulated in certain ways.

2. Staining of Fixed Cells (Immunocytochemistry)

Another routine use of these phospho-specific antibodies, is to test if the antibody is able to demonstrate similar performance when used on fixed cells (intact cells that have been preserved) as it did in the Western blot tests. It is an important aspect in many cases to confirm that the antibody works in actual intact cell samples. Ideally, the method used for cellular fixation should be the same as what is used in pathology labs (like using 10% formalin). To check if the antibody works well in tissue sections (FFPE), researchers will often test it on fixed cells that are processed similar to tissue samples.

3. Specificity Tests Using Peptides

In order to make sure that the antibody is only binding to the right target:

• Laboratory technicians will mix the antibody with phospho-peptides (short segments of the protein containing the phosphate group modification).


• If the antibody signal disappears, it is confirmation that it is binding to the correct phosphorylated location.


• A more robust test is to use both the phosphorylated and non-phosphorylated (dephosphorylated) versions of the protein. The antibody should react only with the phosphorylated one.


• Another method sometimes utilized is to treat the sample with an enzyme, such as alkaline phosphatase, that specifically removes phosphate groups. If the antibody signal disappears after this, it also confirms specificity.

4. Genetic Confirmation

As a final step, scientists can genetically manipulate the nucleotide sequence and alter the target protein by removing the exact site where phosphorylation happens. If the antibody no longer appears to detect the modified protein, it is strong evidence supporting the antibody being specific for that phosphorylated site.

Why Buy Phospho Antibodies Through Us?

• The production laboratory adheres to strict and consistent protocols prior to releasing any of these phospho-specific antibodies:


• Standard methods and proper controls in all tests to ensure high quality.


• These antibodies are tested and validated in different cell types and species.


• High quality control criterion to ensure each batch is consistent, so you will obtain reliable results every time.

FAQ

1. What Are Phospho-Specific Antibodies?

Phospho-specific antibodies are made to detect proteins only when they have a phosphate group linked to a specific amino acid residue. This empowers scientists understand if a protein is "turned on" or active, based on its phosphorylation state.

2. How to Detect Phosphorylated Proteins in a Western Blot?

To find out if a protein is phosphorylated using Western blot:

• Use a phospho-specific antibody that binds only to the phosphorylated form of the protein.


• You can also use a “regular” antibody for the same amino acid sequence of the protein that the phospho-specific antibody is binding to (but in this case, this antibody will not bind if there is a phosphate group present) in order to compare how much of it is phosphorylated versus how much is non-phosphorylated (or “total” protein, if the “normal” antibody’s epitopes are non-phospho-site-specific).

3. How to Choose the Best Antibody?

Here are some simple tips to help you pick the right antibody:

• Know your target


• Match your sample characteristics


• Confirm the intended use is appropriate


• Check “host” and “type”


• Check the “quality” of the presented data/images


• Appraise whether the available validation meets your needs