WB (Western Blot)
Rabbit Phospho-MERTK Polyclonal Antibody | anti-MERTK antibody
Phospho-MERTK Antibody
Gene Names
Mertk; rdy
Reactivity
Human, Monkey, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
Synonyms
Phospho-MERTK, Antibody; Phospho-MERTK Antibody; c MER; c mer proto oncogene tyrosine kinase; Eyk; MER; MERPEN; Mertk; MERTK c-mer proto-oncogene tyrosine kinase; MGC133349; nmf12; Nyk; Proto oncogene tyrosine protein kinase MER; Receptor tyrosine kinase MerTK; RP38; STK kinase; Tyrosine-protein kinase Mer antibody; anti-MERTK antibody
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat
Clonality
Polyclonal
Form/Format
Affinity Purified Immunoglobulins
Sequence Length
994
Applicable Applications for anti-MERTK antibody
WB (Western Blot), IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry), ELISA
Immunogen
Phosphorylated Synthetic peptide corresponding to unique amino acid sequence on MERTK.
Preparation and Storage
Store at -20°C for long term storage
WB (Western Blot)
WB (Western Blot)
Related Product Information for anti-MERTK antibody
Tyrosine kinase activity is critical for transducing mitogenic signals inside the cells. Many of these tyrosine kinase proteins and receptors undergo auto-phosphorylation for the regulation of their activity. Such auto phosphorylation takes place at multiple sites and results in to phosphorylation of cellular substrates. The Nyk receptor tyrosine kinase was identified by screening human glioma expression library with an anti-Phospho tyrosine antibody. The same kinase was also independently identified and cloned by Graham and coworkers and they referred it as Mer. Chicken homologues for this gene are also identified as c-eyk. The Nyk/Mertk/c-eyk are the newest members of the Ufo/Axl family of receptor tyrosine kinases which are characterized by the presence of NCAM-like extra-cellular regions with 2 IgG-like domains juxtaposed with 2 fibronectin III-like regions. It is been shown that MERTK/Nyk has a strong mitogenic and transforming potential by activating PI 3 kinase, PLCgamma, MAP-kinase pathways.
The auto-phosphorylation of MERTK was mapped on triptych peptide containing amino acids 700-780. The three tyrosine residues lay in the tyrosine kinase domain between sub-domains VII and VIII. It has been shown that kinase activity correlates with auto phosphorylation of MERTK and that mutation of this tyrosine severely crippled the kinase activity. Auto phosphorylation of these regions appears to be a common mechanism for several tyrosine kinases, however, the subtle differences in the pattern of phosphorylation was noted in various other kinase systems. It is also notes that preferable substrate for auto phosphorylation in MERTK is the tyrosine 754, substitution mutation to Phenylalanine exhibited a 90% decrease in auto phosphorylation of MERTK compared to wild type protein
The receptor tyrosine kinase MERTK protein is approximately a 175-210 kDa protein. The MERTK protein has a tyrosine kinase domain closer to the C-terminal region and putative phosphorylation sites. The Anti-Phospho-MERTK-selective antibody was generated against phospho-tyrosine containing peptide from a conserved sequence near the C-terminal end that is unique to MERTK/Nyk protein only. Phospho-specific antibodies were isolated on immobilized phospho and non-phospho antigen affinity matrices. FabGennix also has fully characterized, epitope specific non-phospho-MERTK selective antibodies (MKT-101AP, MKT-112AP and MKT-121AP) in affinity-purified form for confocal, Western blotting and immuno cytochemical analysis. FITC-Conjugated antibodies are available, and antibodies can be conjugated to other fluorescent probes at an extra charge. Western blot positive controls for (PC-PMKT) are available in ready-to-use SDS sample buffer. Synthetic peptide (P-PMKT) is also available for immunodepletion/immunocompetition studies.
The auto-phosphorylation of MERTK was mapped on triptych peptide containing amino acids 700-780. The three tyrosine residues lay in the tyrosine kinase domain between sub-domains VII and VIII. It has been shown that kinase activity correlates with auto phosphorylation of MERTK and that mutation of this tyrosine severely crippled the kinase activity. Auto phosphorylation of these regions appears to be a common mechanism for several tyrosine kinases, however, the subtle differences in the pattern of phosphorylation was noted in various other kinase systems. It is also notes that preferable substrate for auto phosphorylation in MERTK is the tyrosine 754, substitution mutation to Phenylalanine exhibited a 90% decrease in auto phosphorylation of MERTK compared to wild type protein
The receptor tyrosine kinase MERTK protein is approximately a 175-210 kDa protein. The MERTK protein has a tyrosine kinase domain closer to the C-terminal region and putative phosphorylation sites. The Anti-Phospho-MERTK-selective antibody was generated against phospho-tyrosine containing peptide from a conserved sequence near the C-terminal end that is unique to MERTK/Nyk protein only. Phospho-specific antibodies were isolated on immobilized phospho and non-phospho antigen affinity matrices. FabGennix also has fully characterized, epitope specific non-phospho-MERTK selective antibodies (MKT-101AP, MKT-112AP and MKT-121AP) in affinity-purified form for confocal, Western blotting and immuno cytochemical analysis. FITC-Conjugated antibodies are available, and antibodies can be conjugated to other fluorescent probes at an extra charge. Western blot positive controls for (PC-PMKT) are available in ready-to-use SDS sample buffer. Synthetic peptide (P-PMKT) is also available for immunodepletion/immunocompetition studies.
Product Categories/Family for anti-MERTK antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
tyrosine-protein kinase Mer
NCBI Official Synonym Full Names
MER proto-oncogene, tyrosine kinase
NCBI Official Symbol
Mertk
NCBI Official Synonym Symbols
rdy
NCBI Protein Information
tyrosine-protein kinase Mer
UniProt Protein Name
Tyrosine-protein kinase Mer
UniProt Gene Name
Mertk
UniProt Synonym Gene Names
Mer
UniProt Entry Name
MERTK_RAT
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Product Notes
The MERTK mertk (Catalog #AAA75659) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-MERTK Antibody reacts with Human, Monkey, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Phospho-MERTK can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry), ELISA. Researchers should empirically determine the suitability of the MERTK mertk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospho-MERTK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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