Monoclonal Antibodies

Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.

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IF (Immunofluorescence) (Immunofluorescence analysis of RAW264.7 cells using CD68 Rabbit PolymAb® (AAA28567) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human tonsil using CD68 Rabbit PolymAb® (AAA28567) at dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human lung using CD68 Rabbit PolymAb® (AAA28567) at dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human liver using CD68 Rabbit PolymAb® (AAA28567) at dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human colon using CD68 Rabbit PolymAb® (AAA28567) at dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

WB (Western Blot) (Western blot analysis of various lysates, using CD68 Rabbit PolymAb® (AAA28567) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Negative control (NC): JurkatExposure time: 10s.)

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CD68, Monoclonal Antibody (Cat# AAA28567)

Full Name

CD68 Rabbit PolymAb

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

Affinity purification

Pricing

$285/0.02 mL $500/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,780/4x0.1 mL $1,960/5x0.1 mL

WB (Western Blot) (Western blot analysis of HEK293 cells transfected with RFP-tagged protein vector. 1:1000 (1ug/mL) Ab dilution. Non·-transfected (1). transfected with TurboRFP (2) and transfected with dsRed (3))

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RFP Tag, Monoclonal Antibody (Cat# AAA14614)

Full Name

RFP Tag Mouse Monoclonal Antibody (RF5R)

Applications

Immunohistochemistry, Western Blot

Pricing

$415/0.1 mg $785/2x0.1 mg $1,130/3x0.1 mg $1,455/4x0.1 mg $1,475/5x0.1 mg

IHC (Immunohistochemistry) (Anti-TCR Gamma/Delta antibody IHC of mouse spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.)

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TCR Gamma+TCR Delta, Monoclonal Antibody (Cat# AAA12360)

Full Name

Armenian Hamster Monoclonal [clone GL-3] (IgG) to Mouse TCR Gamma+TCR Delta

Reactivity

Mouse

Applications

Immunohistochemistry, Flow Cytometry

Purity

Protein G Purified

Pricing

$690/0.1 mg $1,325/2x0.1 mg $1,915/3x0.1 mg $2,475/4x0.1 mg $2,800/5x0.1 mg

ELISA (ELISA analysis was performed by loading 100 uL per well of an anti-Human Fab (CH1) mAb at a concentration of 5, 2.5, 1.25, 0.625, 0.3125, 0.15625, 0.078, and 0.039 ug/mL across 100 ng per well of a human antibody pre-coated plate. The plate was incubated for 1 hour at 37°C. Detection was performed using TMB Substrate for 10 minutes at room temperature in the dark. The plate was stopped with 2M sulfuric acid. Absorbances were read on a spectrophotometer at 450 nm.)

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Fab (CH1), Monoclonal Secondary Antibody (Cat# AAA27977)

Full Name

Anti-Human Fab (CH1) mAb, HRP

Applications

Western Blot

Pricing

$340/0.1 mL $635/2x0.1 mL $910/3x0.1 mL $1,155/5x0.1 mL $1,170/4x0.1 mL

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Application Data (Frozen mouse spleen stained with Rat anti Mouse CD169)

Application Data (Immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse CD169 antibody clone 3D6.112 , green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue using DAPI. High power)

Application Data (Mouse peritoneal macrophages stained with Rat anti Mouse CD169:FITC)

Application Data (Immunoperoxidase staining of Mouse lymph node cryosection stained with Rat anti Mouse CD169 antibody, clone 3D6.112 followed by Goat anti Rat IgG antibody as a detection reagent. Medium power)

Application Data (Immunoperoxidase staining of Mouse lymph node cryosection stained with Rat anti Mouse CD169 antibody, clone 3D6.112 followed by Goat anti Rat IgG antibody as a detection reagent. High power)

Application Data (Immunoperoxidase staining of Mouse lymph node cryosection stained with Rat anti Mouse CD169 antibody, clone 3D6.112 followed by Goat anti Rat IgG antibody as a detection reagent. Low power)

Application Data (Immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse CD169 antibody clone 3D6.112 , green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue using DAPI. Low power)

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CD169, Monoclonal Antibody (Cat# AAA12207)

Full Name

RAT ANTI MOUSE CD169

Gene Names

Siglec1; Sn; Cd169; Siglec-1

Applications

Immunohistochemistry, Flow Cytometry, Immunofluorescence

Pricing

$480/0.1 mg $915/2x0.1 mg $1,325/3x0.1 mg $1,705/4x0.1 mg $1,865/5x0.1 mg

Indirect ELISA (Indirect ELISA:Antigen: Dilution series of Cryptococcus neofonnans.Antibody: MAb AAA13391 @ 5 ~g/mL, incubated 10 minutes at room temp., followed by Donkey anti-Mouse IgG: HRP conjugate and TMB Substrate.)

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Cryptococcus neoformans, Monoclonal Antibody (Cat# AAA13391)

Full Name

MAb to Cryptococcus neoformans
Monoclonal Antibody to Cryptococcus neoformans.

Applications

ELISA

Purity

> 90% pure (SDS-PAGE). Protein A chromatography
Product is 0.2um filtered.

Pricing

$580/1 mg $1,100/2x1 mg $1,595/3x1 mg $2,055/4x1 mg $2,275/5x1 mg

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IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using RELB mouse mAb with DAB staining.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using RELB mouse mAb with DAB staining.)

FCM (Flow Cytometry) (Flow cytometric analysis of MCF-7 cells using RELB mouse mAb (green) and negative control (red).)

IF (Immunofluorescence) (Immunofluorescence analysis of MCF-7 cells using RELB mouse mAb (green). Blue)

WB (Western Blot) (Western blot analysis using RELB mAb against HEK293 (1) and RELB (AA)

WB (Western Blot) (Western blot analysis using RELB mAb against human RELB (AA)

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RELB, Monoclonal Antibody (Cat# AAA14155)

Full Name

Anti-RELB Mouse mAb

Gene Names

RELB; IREL; I-REL; REL-B

Reactivity

Human

Applications

Immunohistochemistry, Immunocytochemistry, Flow Cytometry

Purity

Ascitic Fluid

Pricing

$565/0.1 mL $1,070/2x0.1 mL $1,545/3x0.1 mL $1,900/5x0.1 mL $1,995/4x0.1 mL

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ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-CARD8 mAb. [Lot No. 2108C2a-1])

FCM (Flow Cytometry) (HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-CARD8 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. 2108C2a-1])

IP (Immunoprecipitation) (Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-CARD8 mAb. [Lot No. 2108C2a-1]Predicted molecular weight: 48 kDa)

WB (Western Blot) (Detection of human CARD8 by Western blot.Samples: Whole cell lysate (50 ug) from HeLa cells. [Lot No. 2108C2a-1]Predicted molecular weight: 48 kDa)

Quality Control (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)

WB (Western Blot) (Western blot analysis of immunized recombinant protein, using anti-CARD8 monoclonal antibody.)

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CARD8, Monoclonal Antibody (Cat# AAA10604)

Full Name

Mouse monoclonal antibody Anti-Human CARD8

Gene Names

CARD8; NDPP; DACAR; DAKAR; NDPP1; TUCAN; CARDINAL

Reactivity

Human

Applications

Western Blot, Immunoprecipitation, Flow Cytometry, Immunocytochemistry

Purity

This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.

Pricing

$615/0.1 mg $1,170/2x0.1 mg $1,695/3x0.1 mg $2,190/4x0.1 mg $2,435/5x0.1 mg

Application Data (Figure 1. Immunostaining of human tonsil tissue in paraffin section with HC10 (dilution 1:20.000).)

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HLA Class I Heavy Chain, Monoclonal Antibody (Cat# AAA14567)

Full Name

Mouse anti Human HLA Class I Heavy Chain (Restricted expression)

Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot

Pricing

$645/0.1 mg $1,230/2x0.1 mg $1,780/3x0.1 mg $2,305/4x0.1 mg $2,590/5x0.1 mg

IHC (Immunohistochemistry) (Anti-ITGB2 / CD18 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

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ITGB2 / MAC-1 / CD18, Monoclonal Antibody (Cat# AAA12341)

Full Name

Mouse Monoclonal [clone MEM-48] (IgG1) to Human ITGB2 / MAC-1 / CD18

Gene Names

ITGB2; LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1

Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Immunoprecipitation, Flow Cytometry, Functional Assay

Purity

Purified by Protein A affinity chromatography.
>95% by SDS-PAGE.

Pricing

$690/0.05 mg $1,325/2x0.05 mg $1,915/3x0.05 mg $2,475/4x0.05 mg $2,800/5x0.05 mg

Application Data

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H-2Db, Monoclonal Antibody (Cat# AAA14272)

Full Name

Anti-Mouse H-2Db, Affinity Purified (Clone B22-249.R1) (mouse IgG2a)

Reactivity

Mouse

Applications

Cytotoxic Assay

Pricing

$450/0.25 mg $855/2x0.25 mg $1,230/3x0.25 mg $1,595/4x0.25 mg $1,645/5x0.25 mg

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IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using GAPDH Mouse mAb (AC002) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse kidney using GAPDH Mouse mAb (AAA10621) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human kidney using GAPDH Mouse mAb (AAA10621) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

WB (Western Blot) (Western blot analysis of lysates from HeLa cells using GAPDH Mouse mAb (AAA10621) at1:5000-1:1280000 dilution incubated overnight at 4°C.Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) at 1:10000dilution.Lysates/proteins: 25 µg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic KitExposure time: 20 s.)

WB (Western Blot) (Western blot analysis of various lysates using GAPDH Mouse mAb (AAA10621) at 1:10000dilution incubated overnight at 4°C.Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) at 1:10000dilution.Lysates/proteins: 25?g per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using GAPDH Monoclonal Antibody (AAA10621) at dilution of 1:6 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using GAPDH Monoclonal Antibody (AAA10621) at dilution of 1:6 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 cells using GAPDH Monoclonal Antibody (AAA10621) at dilution of 1:6 (40x lens). Blue: DAPI for nuclear staining.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using GAPDH antibody (AAA10621) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)

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GAPDH, Monoclonal Antibody (Cat# AAA10621)

Full Name

GAPDH Monoclonal Antibody

Gene Names

GAPDH; G3PD; GAPD; HEL-S-162eP

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

Affinity Purification

Pricing

$290/0.1 mL $530/2x0.1 mL $755/3x0.1 mL $965/5x0.1 mL $975/4x0.1 mL

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Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)

Application Data (Published customer image: Post-injury 7,8-dihydroxyflavone treatment increased brain BDNF levels and promoted CREB activation. (A) Bar graphs demonstrating brain derived neurotrophic factor (BDNF) protein concentrations measured by enzyme-linked immunosorbent assay (ELISA) in sham-injured, vehicle-treated, and 20 mg/kg 7,8-dihydroxyflavone (DHF 20)-treated mice at 4 days post-TBI. DHF 20 significantly increased BDNF protein levels in the ipsilateral hemisphere at 4 days post-injury. (B) Bar graphs demonstrating BDNF mRNA expression measured by real -time quantitative reverse transcriptase polymerase chain reaction (RT-PCR) at 1 and 4 days post-injury. DHF 20 significantly increased BDNF mRNA levels in the ipsilateral hemisphere at 4 days post-injury. (C) Western blot analysis of the phospho-CREB level in the ipsilateral hemisphere of sham-injured, vehicle-treated, and DHF 20-treated mice at 1 h, 1 day, and 4 days post-injury. DHF 20 enhanced CREB phosphorylation at 4 days post-injury. (D) Identification of phospho-CREB-positive cells 4 day post-injury in the peri-contusional margin by double immunofluorescence staining. Phospho-CREB is shown in red, and NeuN (neurons), GFAP (astrocytes), or F4/80 (microglia) is shown in green. Co-localization of phospho-CREB with NeuN is shown by yellow labeling. Sections were stained with DAPI (blue) to show all nuclei. Values are mean +/- SEM; *P)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen: Pacific Blue(AAA12225PB))

Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen:Biotin)

Application Data (Frozen mouse spleen stained with A: Rat anti Mouse F4/80 followed by Goat anti Rat IgG:HRP or B: Rat anti Mouse F4/80 preincubated with 2 molar excess of Human anti Idiotypic AAA12225 (HCA154) followed by Goat anti Rat IgG:HRP)

Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen:Low Endotoxin)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen: RPE - Alexa Fluor 750 (AAA12225P750))

Application Data (Staining of mouse peritoneal macrophages cells with Rat anti Mouse F4/80 antigen:APC)

Application Data (Immunofluorescence image of mouse small intestine stained with Rat anti Mouse F4/80 , red and Rat anti Mouse CD45 , green; nuclei are stained with DAPI, blue. The image higlights macrophages within the lamina propria)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen:FITC)

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F4/80, Monoclonal Antibody (Cat# AAA12225)

Full Name

RAT ANTI MOUSE F4/80

Gene Names

Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry, Immunohistochemistry, Immunoprecipitation, Immunofluorescence, Radioimmunoassay, Fluorescence Microscopy

Pricing

$715/2 mL $1,375/2x2 mL $1,990/3x2 mL $2,575/4x2 mL $2,920/5x2 mL

Application Data

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gH/gL/gp42 (EBV), Monoclonal Antibody (Cat# AAA13705)

Full Name

Anti-gH/gL/gp42 Complex (EBV) Mouse Monoclonal Antibody

Applications

Immunoprecipitation, Immunofluorescence

Purity

Protein G chromatography

Pricing

$565/0.1 mg $1,070/2x0.1 mg $1,545/3x0.1 mg $1,995/4x0.1 mg $2,230/5x0.1 mg

WB (Western Blot) (Western blot analysis of HUVECs untreated (lanes 1 & 3) or treated with alkaline phosphatase (lanes 2 & 4). Blots were probed with mouse monoclonal anti-FAK (lanes 1 & 2) and anti-FAK (Tyr-397) (lanes 3 & 4).)

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FAK (Tyr-397), Monoclonal Antibody (Cat# AAA14099)

Full Name

FAK (Tyr-397), phospho-specific, Mouse Monoclonal IgG1

Reactivity

Human, Rat, Mouse, Rabbit

Applications

Western Blot

Purity

Protein A Purified

Pricing

$535/0.1 mL $1,015/2x0.1 mL $1,470/3x0.1 mL $1,900/4x0.1 mL $2,125/5x0.1 mL

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IF (Immunofluorescence) (Figure 3 and 4: ICC/IF analysis of MIF in Balb/3T3 cells. The cell was stained with AAA11724 (1;100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucelus (blue).Figure 5 and 6: ICC/IF analysis of MIF in HeLa cells line. The cell was stained with AAA11724 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue))

WB (Western Blot) (Figure 1: The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MIF antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Jurkat cell lysateLane 2.: THP-1 cell lysateLane 3.: HeLa cell lysateLane 4.: U937 cell lysateFigure 2: The cell lysates of HL-60 was resolved by SDS-PAGE, transfered to PVDF membrane and probed with anit-human MIF antibody (1:1000). Protein was visualized using a goat anti-mouse secondary antibody conjugated to HRP and ECL detection system.)

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MIF, Monoclonal Antibody (Cat# AAA11724)

Full Name

MIF antibody

Gene Names

MIF; GIF; GLIF; MMIF

Reactivity

Human

Applications

Western Blot, Immunocytochemistry, Immunofluorescence

Purity

By protein-G affinity chromatography

Pricing

$320/0.05 mL $405/0.1 mL $760/2x0.1 mL $1,040/5x0.05 mL $1,105/3x0.1 mL $1,420/4x0.1 mL $1,425/5x0.1 mL

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Application Data (Published customer image:RPE conjugated Mouse anti Canine CD4 antibody, clone YKIC302.9used for the assessment of CD4 levels on canine cells by flow cytometry.Image caption:Immunophenotypic profile of tumor infiltrating lymphocyte in canine mammary carcinomas. Analysis of tumor infiltrating T-cells, B-lymphocytes and T-cell subsets from MC-BMT or MC (A), further subcategorized according to the absence (-) or presence (+) of lymph node metastasis (-) (B). Lymphocyte populations and subsets were identified by flow cytometric immunostaining as described in Material and Methods. Data were expressed as percentage of positive cells within gated lymphocytes and CD4+/CD8+ T-cell ratio. Significant differences at p < 0.05 are highlighted by asterisk.From: From: Estrela-Lima et al.BMC Cancer 2010 10:256.)

Application Data (Published customer image:Pacific Blue conjugated Mouse anti Canine CD4 antibody, clone YKIX302.9 used for evaluation of CD4 expression on canine cells by flow cytometry.Image caption:CD11b+CD14-MHCII- cells suppress T cell proliferation. Facs sorted CD11b+CD14-MHCII- cells isolated from a dog with osteosarcoma or healthy PBMCs were co-incubated with mitogen-stimulated CD4+ and CD8+ T cells isolated from a healthy dog for 72 hrs. No stimulated cells were used as negative control. Proliferative responses were measured by 3H-thymidine incorporation from experiments performed in triplicate. CPM, counts per minute. Mean +/- SEM are shown.From: Goulart MR, Pluhar GE, Ohlfest JR (2012)Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer.PLoS ONE 7(3): e33274.)

Application Data (Figure A. Purified Mouse anti Canine CD8b detected with Goat anti Mouse IgG1 DyLight 649 and Rat IgG2b FITC isotype control . Figure B. Purified Mouse anti Canine CD8b detected with Goat anti Mouse IgG1 PE and Rat anti Canine CD4 FITC . All experiments performed on red cell lysed canine blood gated on mononuclear cells.)

Application Data (Figure A. A488 conjugated Rat anti Canine CD4 and purified Mouse IgG1 isotype control detected with Goat anti Mouse IgG1 DyLight 649 . Figure B. A488 conjugated Rat anti Canine CD4 and purified Mouse anti Canine CD8b detected with Goat anti Mouse IgG1 DyLight 649 . All experiments performed on red cell lysed canine blood gated on mononuclear cells.)

Application Data (Figure A. RPE conjugated Mouse anti Canine CD5 and A647 conjugated Rat IgG2a isotype control . Figure B. RPE conjugated Rat anti Canine CD5 and A647 conjugated Rat anti Canine CD4 . All experiments performed on red cell lysed canine blood gated on mononuclear cells.)

Application Data (Figure A. A647 conjugated Rat anti Canine CD4 and RPE conjugated Rat IgG2a isotype control . Figure B. A647 conjugated Rat anti Canine CD4 and RPE conjugated Rat anti Canine CD5 . All experiments performed on red cell lysed canine blood gated on mononuclear cells.)

Application Data (Figure A. RPE conjugated Rat anti Canine CD4 and FITC conjugated Mouse IgG1 isotype control . Figure B. RPE conjugated Rat anti Canine CD4 and FITC conjugated Mouse anti Canine CD3 . All experiments performed on red cell lysed canine blood gated on mononuclear cells.)

Application Data (Figure A. FITC conjugated Mouse anti Canine CD3 and RPE conjugated Rat IgG2a isotype control . Figure B. FITC conjugated Mouse anti Canine CD3 and RPE conjugated Rat anti Canine CD4 . All experiments performed on red cell lysed canine blood gated on mononuclear cells.)

Application Data (Figure A. Alexa Fluor 647 conjugated Rat anti Canine CD5 and Alexa Fluor 488 conjugated Rat IgG2a isotype control . Figure B. Alexa Fluor 647 conjugated Mouse anti Canine CD5 and Alexa Fluor 488 conjugated Rat anti Canine CD4 . All experiments performed on red cell lysed canine blood gated on lymphocytes in the presence of 10% canine serum. Data acquired on the ZE5 Cell Analyzer.)

Application Data (Figure A. Alexa Fluor 647 conjugated Rat anti Dog CD8 and RPE conjugated Rat IgG2a isotype control . Figure B. Alexa Fluor 647 conjugated Rat anti Dog CD8 and RPE conjugated Rat anti Dog CD4 . All experiments performed on red cell lysed canine blood gated on lymphoid cells in the presence of 10% dog serum. Data acquired on the ZE5 Cell Analyzer.)

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CD4, Monoclonal Antibody (Cat# AAA12279)

Full Name

RAT ANTI DOG CD4: RPE-Cy7

Applications

Flow Cytometry

Purity

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Pricing

$795/100 Tests $1,520/2x100 Tests $2,210/3x100 Tests $2,860/4x100 Tests $3,250/5x100 Tests

Application Data (HLA region of Chromosome 6)

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HLA Class 1 Antigen A30,A31, Monoclonal Antibody (Cat# AAA14671)

Full Name

HLA Class 1 Antigen A30,A31 (Biotin)

Gene Names

HLA-A; HLAA

Reactivity

Human

Applications

Flow Cytometry

Purity

Purified by Ion Exchange chromatography.

Pricing

$1,650/0.25 mL $3,190/2x0.25 mL $4,645/3x0.25 mL $6,020/4x0.25 mL $7,130/5x0.25 mL

What are Monoclonal Antibodies?

Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.

This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.

AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.

What Are The Uses of Monoclonal Antibodies

Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.

ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.

Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.

Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.

Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.

How Monoclonal Antibodies Are Used as Medicine?

Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).

Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:

• Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.


• Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.


• Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.

Why Buy Monoclonal Antibodies From Us?

At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.

1. Validated for Versatile Applications

The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).

2. Wide Selection & Specialized Options

We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.

3. High-Quality Proteins

Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.

Frequently Asked Questions

1. Are your monoclonal antibodies validated for specific applications?

Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.

2. How do I choose the right monoclonal antibody for my application?

Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.

3. How quickly can I receive my order?

Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.