Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD169, clone ED3 followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a as a detection reagent. High power)
Application Data (Frozen rat spleen stained with Mouse anti Rat CD169 followed by Goat anti Mouse IgG:HRP (Rat Adosorbed, Data)
Application Data (Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD169, clone ED3 , red in A and Mouse anti Rat CD4, clone W3/25 , green in B. C is the merged image with nuclei counter-staind blue using DAPI. Medium power)
Application Data (Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD169, clone ED3 , red in A and Mouse anti Rat CD4, clone W3/25 , green in B. C is the merged image with nuclei counter-staind blue using DAPI. High power)
Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD169, clone ED3 followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a as a detection reagent. Medium power)
Application Data (Published customer image: Systemically administered liposomes home primarily to splenic marginal zone and red pulp macrophages. (a,b) Healthy rats were injected with 5 mg/kg DiI-labeled PCLs and PSLs. Splenic cryosections were stained with CD169 (a, marginal metallophilic and marginal zone macrophages) and CD68 (b, red pulp macrophages). One representative experiment is shown (20x magnification).From: Bogie JF, Jorissen W, Mailleux J, Nijland PG, Zelcer N, Vanmierlo T, Van Horssen J, Stinissen P, Hellings N, Hendriks JJ. Myelin alters the inflammatory phenotype of macrophages by activating PPARs. Acta Neuropathol Commun. 2013 Aug 2;1(1):43.)
Application Data (Published customer image: Immunostaining of rat spleen. Serial-cut frozen sections were stained with mAbs towards A, rat MCL B, rat CD169 C, rat MHC class II D, human MHC class I (negative control) and visualized with peroxidase-conjugated secondary antibody and DAB substrate. RP: red pulp. PALS: periarteriolar lymphoid sheath. FOLL: follicle. MZ: marginal zone.From: Lobato-Pascual A, Saether PC, Dahle MK, Gaustad P, Dissen E, et al. (2013) Rat Macrophage C-Type Lectin Is an Activating Receptor Expressed by Phagocytic Cells. PLoS ONE 8(2): e57406.)
Application Data (Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD169, clone ED3 , red in A and Mouse anti Rat CD4, clone W3/25 , green in B. C is the merged image with nuclei counter-staind blue using DAPI. Low power)
ICC (Immunocytochemistry) (Confocal imaging of paraffin-embedded Mouse brain tissue using ?III-Tubulin/?3-Tubulin Rabbit mAb (AAA28465, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining.)
ICC (Immunocytochemistry) (Confocal imaging of paraffin-embedded Rat brain tissue using ?III-Tubulin/?3-Tubulin Rabbit mAb (AAA28465, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using ?III-Tubulin Rabbit mAb (AAA28465) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using ?III-Tubulin Rabbit mAb (AAA28465) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
ICC (Immunocytochemistry) (Confocal imaging of SH-SY5Y cells using ?III-Tubulin Rabbit mAb (AAA28465, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with ?-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.)
WB (Western Blot) (Western blot analysis of various lysates using ?III-Tubulin Rabbit mAb (AAA28465) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 3min.)
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
Supernatant
Pricing
Application Data (Figure: Biopsy of tumor lesion from a patient with lymphoma (DLBCL) stained with MAb to APRIL (Aprily-2) . The biopsy was stained by standard immunohistochemistry. The figure shows the selective accumulation of APRIL around tumor cells.Picture courtesy of Dr. Bertrand Huard (University of Geneva).)
IF (Immunofluorescence) (Immunofluorescence Analysis of PFA-fixed U87MG cells stained using CD63 Mouse Monoclonal Antibody (MX-49.129.5) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).)
IF (Immunofluorescence) (IF staining of HeLa cells using AF488 labeled CD63 Monoclonal Antibody (MX-49.129.5) (green). F-actin filaments are labeled with Dylight 554 phalloidin (red). Nuclei stained with DAPI (blue).)
FCM (Flow Cytometry) (Flow cytometric analysis of bead-bound exosomes derived from MCF-7 cells. CD63 Mouse Monoclonal Antibody (MX-49.129.5) followed by goat anti-mouse IgG-CF568 (orange); unstained exosomes (gray).)
FCM (Flow Cytometry) (Flow cytometric analysis of bead-bound exosomes derived from MCF-7 cells. CD63 Mouse Monoclonal Antibody (MX-49.129.5) followed by goat anti-mouse IgG-CF488 (green); unstained exosomes (gray).)
FCM (Flow Cytometry) (Flow cytometric analysis of MCF-7 cells. CD63 Mouse Monoclonal Antibody (MX-49.129.5) followed by goat anti-mouse IgG-CF488 (green); unstained cells (gray).)
FCM (Flow Cytometry) (Flow Cytometric Analysis of trypsinized MeOH-fixed HeLa cells using Cytokeratin 14 Mouse Monoclonal Antibody (LL002) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).)
IF (Immunofluorescence) (mmunofluorescence Analysis of A549 cells labeling CK14 with Cytokeratin 14 Mouse Monoclonal Antibody (LL002) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).)
SDS-PAGE (SDS-PAGE Analysis Purified Cytokeratin 14 Mouse Monoclonal Antibody (LL002). Confirmation of Purity and Integrity of Antibody.)
Application Data (Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin 14 Mouse Monoclonal Antibody (LL002).)
Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD169, clone ED3 followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a as a detection reagent. High power)
Application Data (Frozen rat spleen stained with Mouse anti Rat CD169 followed by Goat anti Mouse IgG:HRP (Rat Adosorbed, Data)
Application Data (Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD169, clone ED3 , red in A and Mouse anti Rat CD4, clone W3/25 , green in B. C is the merged image with nuclei counter-staind blue using DAPI. Medium power)
Application Data (Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD169, clone ED3 , red in A and Mouse anti Rat CD4, clone W3/25 , green in B. C is the merged image with nuclei counter-staind blue using DAPI. High power)
Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD169, clone ED3 followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a as a detection reagent. Medium power)
Application Data (Published customer image: Systemically administered liposomes home primarily to splenic marginal zone and red pulp macrophages. (a,b) Healthy rats were injected with 5 mg/kg DiI-labeled PCLs and PSLs. Splenic cryosections were stained with CD169 (a, marginal metallophilic and marginal zone macrophages) and CD68 (b, red pulp macrophages). One representative experiment is shown (20x magnification).From: Bogie JF, Jorissen W, Mailleux J, Nijland PG, Zelcer N, Vanmierlo T, Van Horssen J, Stinissen P, Hellings N, Hendriks JJ. Myelin alters the inflammatory phenotype of macrophages by activating PPARs. Acta Neuropathol Commun. 2013 Aug 2;1(1):43.)
Application Data (Published customer image: Immunostaining of rat spleen. Serial-cut frozen sections were stained with mAbs towards A, rat MCL B, rat CD169 C, rat MHC class II D, human MHC class I (negative control) and visualized with peroxidase-conjugated secondary antibody and DAB substrate. RP: red pulp. PALS: periarteriolar lymphoid sheath. FOLL: follicle. MZ: marginal zone.From: Lobato-Pascual A, Saether PC, Dahle MK, Gaustad P, Dissen E, et al. (2013) Rat Macrophage C-Type Lectin Is an Activating Receptor Expressed by Phagocytic Cells. PLoS ONE 8(2): e57406.)
Application Data (Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD169, clone ED3 , red in A and Mouse anti Rat CD4, clone W3/25 , green in B. C is the merged image with nuclei counter-staind blue using DAPI. Low power)
ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-YARS mAb. [Lot No. YAR5H08-1])
FCM (Flow Cytometry) (HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-YARS mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. YAR5H08-2])
IP (Immunoprecipitation) (Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-YARS mAb. [Lot No. YAR5H08-2]Predicted molecular weight: 59 kDa)
WB (Western Blot) (Detection of YARS by Western blot.Samples: Whole cell lysate from human HeLa (H, 50 ug), mouse NIH3T3 (M, 50 ug) and rat F2408 (R, 50 ug) cells. [Lot No. YAR5H08-2])
Quality Control (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control (Western blot analysis of immunized recombinant protein, using anti-YARS monoclonal antibody.)
FCM (Flow Cytometry) (Flow cytometric analysis of 2% paraformaldehyde-fixed THP1 (Human acute monocytic leukemia cell line) cells labeling PIEZO1 with AAA13800 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, green). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.)
IF (Immunofluorescence) (Huvecs transfected with Piezo1-GFP, Staining with mouse anti-human Piezo1 antibody (5 ug/ml, AAA13800). No Permeablized. Recommended dilution: 10 ug/mlIF-Anti-PIEZO1 Antibody AAA13800)
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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