Monoclonal Antibodies

Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.

WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)

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PGRMC2, Monoclonal Antibody (Cat# AAA24312)

Full Name

PGRMC2 (Membrane-associated Progesterone Receptor Component 2, Progesterone Membrane-binding Protein, Steroid Receptor Protein DG6, DG6, PMBP) (AP)

Gene Names

PGRMC2; DG6; PMBP

Reactivity

Human, Mouse, Rat

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged MEIS2 is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MEIS2 on HeLa cell. [antibody concentration 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to MEIS2 on formalin-fixed paraffin-embedded human spleen tissue. [antibody concentration 1ug/ml])

WB (Western Blot) (Western Blot analysis of MEIS2 expression in transfected 293T cell line by MEIS2 monoclonal antibody. Lane 1: MEIS2 transfected lysate (51.1kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (MEIS2 monoclonal antibody Western Blot analysis of MEIS2 expression in K-562.)

WB (Western Blot) (MEIS2 monoclonal antibody Western Blot analysis of MEIS2 expression in HeLa.)

WB (Western Blot) (Western Blot detection against Immunogen (67.65kD).)

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MEIS2, Monoclonal Antibody (Cat# AAA24567)

Full Name

MEIS2 (Homeobox Protein Meis2, Meis1-related Protein 1, MRG1, MGC2820) APC

Gene Names

MEIS2; MRG1; HsT18361

Reactivity

Human

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Analysis of Protein Array containing more than 19,000 full-length human proteins using Calretinin Mouse Monoclonal Antibody (CALB2/2786). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

SDS-PAGE (SDS-PAGE Analysis of Purified Calretinin Mouse Monoclonal Antibody (CALB2/2786). Confirmation of Purity and Integrity of Antibody.)

WB (Western Blot) (Western Blot Analysis of human Brain tissue lysate using Calretinin Mouse Monoclonal Antibody (CALB2/2786).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Mesothelioma stained with Calretinin Mouse Monoclonal Antibody (CALB2/2786).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Calretinin Mouse Monoclonal Antibody (CALB2/2786).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Cerebellum stained with Calretinin Mouse Monoclonal Antibody (CALB2/2786).)

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Calretinin/Calbindin 2, Monoclonal Antibody (Cat# AAA23939)

Full Name

Calretinin/Calbindin 2 (Mesothelioma Marker)

Gene Names

CALB2; CR; CAL2; CAB29

Reactivity

Human

Applications

WB, IHC

Purity

Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA at 1.0mg/ml

Pricing

$295/0.02 mg (With BSA & Azide at 0.2mg/mL) $475/0.1 mg (With BSA & Azide at 0.2mg/mL) $475/0.1 mg (Without BSA at 1mg/mL) $905/2x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,310/3x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,690/4x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,900/5x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,900/5x0.1 mg (Without BSA at 1mg/mL)

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WB (Western Blot) (Western blot analysis of SNX1 over-expressed 293 cell line, cotransfected with SNX1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with SNX1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged SNX1 is ~0.03ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SNX1 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of SNX1 expression in transfected 293T cell line by SNX1 monoclonal antibody. Lane 1: SNX1 transfected lysate (59.1kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (SNX1 monoclonal antibody, Western Blot analysis of SNX1 expression in HeLa.)

WB (Western Blot) (Western Blot detection against Immunogen (38.21kD).)

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SNX1, Monoclonal Antibody (Cat# AAA25552)

Full Name

SNX1 (Sorting Nexin-1, MGC8664) (HRP)

Gene Names

SNX1; VPS5; HsT17379

Reactivity

Human

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)

WB (Western Blot) (FGL2 monoclonal antibody Western Blot analysis of FGL2 expression in PC-12.)

Application Data (Detection limit for recombinant GST tagged FGL2 is ~0.1ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of FGL2 transfected lysate using FGL2 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with FGL2 rabbit polyclonal antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to FGL2 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 0.5ug/ml].)

WB (Western Blot) (Western Blot analysis of FGL2 expression in transfected 293T cell line by FGL2 monoclonal antibody Lane 1: FGL2 transfected lysate (50kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (FGL2 monoclonal antibody Western Blot analysis of FGL2 expression in Raw 264.7.)

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FGL2, Monoclonal Antibody (Cat# AAA24800)

Full Name

FGL2 (Fibroleukin, Fibrinogen-like Protein 2, pT49) (Biotin)

Gene Names

FGL2; T49; pT49

Reactivity

Human, Mouse, Rat

Applications

EIA, IHC, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (GLMN monoclonal antibody Western Blot analysis of GLMN expression in HL-60.)

WB (Western Blot) (GLMN monoclonal antibody Western Blot analysis of GLMN expression in Jurkat.)

Application Data (Detection limit for recombinant GST tagged GLMN is ~0.03ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of GLMN transfected lysate using GLMN monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with GLMN rabbit polyclonal antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to GLMN on HeLa cell. [antibody concentration 20ug/ml])

WB (Western Blot) (Western Blot analysis of GLMN expression in transfected 293T cell line by GLMN monoclonal antibody. Lane 1: GLMN transfected lysate (68.2kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (91.45kD).)

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GLMN, Monoclonal Antibody (Cat# AAA24816)

Full Name

GLMN (FAP48, FAP68, VMGLOM, Glomulin, FK506-binding Protein-associated Protein, FAP, FKBP-associated Protein) (Biotin)

Gene Names

GLMN; FAP; GVM; GLML; FAP48; FAP68; FKBPAP; VMGLOM

Reactivity

Human

Applications

EIA, IF, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (FOXA1 monoclonal antibody (M03), clone 4F6 Western Blot analysis of FOXA1 expression in A-431.)

WB (Western Blot) (FOXA1 monoclonal antibody (M03), clone 4F6 Western Blot analysis of FOXA1 expression in HepG2.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FOXA1 on A-431 cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FOXA1 on A-431 cell. [antibody concentration 10 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to FOXA1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml])

Application Data (Immunoperoxidase of monoclonal antibody to FOXA1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml])

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FOXA1, Monoclonal Antibody (Cat# AAA26090)

Full Name

FOXA1 (Forkhead Box A1, HNF3A, MGC33105, TCF3A) (APC)

Gene Names

FOXA1; HNF3A; TCF3A

Applications

IF, IHC, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western blot analysis of ABCF2 over-expressed 293 cell line, cotransfected with ABCF2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ABCF2 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged ABCF2 is ~0.3ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of ABCF2 transfected lysate using anti-ABCF2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ABCF2 monoclonal antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ABCF2 on formalin-fixed paraffin-embedded human prostate. [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot analysis of ABCF2 expression in transfected 293T cell line by ABCF2 monoclonal antibody: Lane 1: ABCF2 transfected lysate (68.53kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (ABCF2 monoclonal antibody Western Blot analysis of ABCF2 expression in Hela.)

WB (Western Blot) (Western Blot detection against Immunogen (37.73kD).)

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ABCF2, Monoclonal Antibody (Cat# AAA25590)

Full Name

ABCF2 (ATP-binding Cassette Sub-family F Member 2, Iron-inhibited ABC Transporter 2, HUSSY-18, DKFZp586K1823, EST133090, M-ABC1) (PE)

Gene Names

ABCF2; ABC28; HUSSY18; HUSSY-18; EST133090

Reactivity

Human

Applications

EIA, IHC, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of SiHa cells using anti-RALBP1 antibody (AAA19684).Overlay histogram showing SiHa cells stained with AAA19684 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-RALBP1 Antibody (AAA19684, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 7. IF analysis of RALBP1 using anti-RALBP1 antibody (AAA19684).RALBP1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL mouse anti-RALBP1 Antibody (AAA19684) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of RALBP1 using anti-RALBP1 antibody (AAA19684).RALBP1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-RALBP1 Antibody (AAA19684) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of RALBP1 using anti-RALBP1 antibody (AAA19684).RALBP1 was detected in a paraffin-embedded section of human metaplasia of squamous cells of the renal pelvis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-RALBP1 Antibody (AAA19684) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of RALBP1 using anti-RALBP1 antibody (AAA19684).RALBP1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-RALBP1 Antibody (AAA19684) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of RALBP1 using anti-RALBP1 antibody (AAA19684).RALBP1 was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-RALBP1 Antibody (AAA19684) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of RALBP1 using anti-RALBP1 antibody (AAA19684).RALBP1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-RALBP1 Antibody (AAA19684) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of RALBP1 using anti-RALBP1 antibody (AAA19684).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human HL-60 whole cell lysates,Lane 2: human Daudi whole cell lysates,Lane 3: human SW620 whole cell lysates,Lane 4: rat brain tissue lysates,Lane 5: mouse lung tissue lysates,Lane 6: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-RALBP1 antigen affinity purified monoclonal antibody (#AAA19684) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RALBP1 at approximately 95 kDa. The expected band size for RALBP1 is at 95 kDa.)

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RALBP1, Monoclonal Antibody (Cat# AAA19684)

Full Name

Anti-RALBP1 Antibody Picoband (monoclonal, 2F9D3)

Gene Names

RALBP1; RIP1; RLIP1; RLIP76

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ICC, IF, FC/FACS

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of U20S cells using anti- ADA antibody (AAA19184). Overlay histogram showing U20S cells stained with AAA19184 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- ADA Antibody (AAA19184, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG (BA1126, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of ADA using anti-ADA antibody (AAA19184). ADA was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml mouse anti-ADA Antibody (AAA19184) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of ADA using anti-ADA antibody (AAA19184). ADA was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml mouse anti-ADA Antibody (AAA19184) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of ADA using anti-ADA antibody (AAA19184). ADA was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml mouse anti-ADA Antibody (AAA19184) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of ADA using anti-ADA antibody (AAA19184). ADA was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml mouse anti-ADA Antibody (AAA19184) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of ADA using anti-ADA antibody (AAA19184). ADA was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml mouse anti-ADA Antibody (AAA19184) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of ADA using anti-ADA antibody (AAA19184). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human A549 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human U-937 whole cell lysates, Lane 6: human U20S whole cell lysates, Lane 7: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-ADA antigen affinity purified monoclonal antibody at 0.5 ug/ml overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system.)

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ADA, Monoclonal Antibody (Cat# AAA19184)

Full Name

Anti-ADA Antibody (monoclonal, 6D4)

Reactivity

Human

Applications

WB, IHC, FC/FACS

Purity

Immunogen Affinity Purified

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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WB (Western Blot) (Western blot analysis of ILK over-expressed 293 cell line, cotransfected with ILK Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ILK monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

WB (Western Blot) (ILK monoclonal antibody. Western Blot analysis of ILK expression in NIH/3T3.)

Application Data (Detection limit for recombinant GST tagged ILK is ~3ng/ml as a capture antibody.)

WB (Western Blot) (ILK monoclonal antibody. Western Blot analysis of ILK expression in HepG2.)

WB (Western Blot) (ILK monoclonal antibody. Western Blot analysis of ILK expression in PC-12.)

WB (Western Blot) (ILK monoclonal antibody Western Blot analysis of ILK expression in HeLa.)

WB (Western Blot) (Western Blot detection against Immunogen (38.06kD).)

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ILK, Monoclonal Antibody (Cat# AAA24541)

Full Name

ILK (Integrin-linked Protein Kinase, 59kD Serine/Threonine-protein Kinase, ILK-1, ILK-2, p59ILK, ILK1, ILK2, DKFZp686F1765) APC

Gene Names

ILK; P59; ILK-1; ILK-2; p59ILK; HEL-S-28

Reactivity

Human, Mouse, Rat

Applications

EIA, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (RAB7B monoclonal antibody. Western Blot analysis of RAB7B expression in A-431.)

Application Data (Detection limit for recombinant GST tagged RAB7B is ~0.03ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of RAB7B transfected lysate using RAB7B monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with RAB7B rabbit polyclonal antibody.)

WB (Western Blot) (Western Blot analysis of RAB7B expression in transfected 293T cell line by RAB7B monoclonal antibody. Lane 1: RAB7B transfected lysate (22.5kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (RAB7B monoclonal antibody. Western Blot analysis of RAB7B expression in human kidney.)

WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)

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RAB7B, Monoclonal Antibody (Cat# AAA25811)

Full Name

RAB7B (Ras-related Protein Rab-7b, RAB7) (PE)

Gene Names

RAB7B; RAB7

Reactivity

Human

Applications

EIA, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (SP1 monoclonal antibody (M01), clone 4H6. Western Blot analysis of SP1 expression in Hela S3 NE.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SP1 on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 1 ~ 10 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SP1 on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 1 ~ 10 ug/ml])

WB (Western Blot) (SP1 monoclonal antibody (M01), clone 4H6 Western Blot analysis of SP1 expression in IMR-32.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SP1 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SP1 on HeLa cell. [antibody concentration 10 ug/ml])

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SP1, Monoclonal Antibody (Cat# AAA26074)

Full Name

SP1 (Sp1 Transcription Factor) (APC)

Applications

EIA, IF, IHC, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (RNF12 monoclonal antibody, Western Blot analysis of RNF12 expression in HeLa NE.)

Application Data (Detection limit for recombinant GST tagged RNF12 is ~0.03ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of RNF12 transfected lysate using RNF12 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with RNF12 rabbit polyclonal antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RNF12 on HeLa cell. [antibody concentration 30ug/ml].)

WB (Western Blot) (Western Blot analysis of RNF12 expression in transfected 293T cell line by RNF12 monoclonal antibody. Lane 1: RNF12 transfected lysate (68.5kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (35.24kD).)

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RLIM, Monoclonal Antibody (Cat# AAA25817)

Full Name

RLIM (RING Finger LIM Domain-binding Protein, R-LIM, E3 Ubiquitin-protein Ligase RLIM, LIM Domain-interacting RING Finger Protein, RING Finger Protein 12, RNF12, Renal Carcinoma Antigen NY-REN-43) (PE)

Gene Names

RLIM; MRX61; RNF12; TOKAS; NY-REN-43

Reactivity

Human

Applications

EIA, IF, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (TRAPPC4 monoclonal antibody. Western Blot analysis of TRAPPC4 expression in NIH/3T3.)

Application Data (Detection limit for recombinant GST tagged TRAPPC4 is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TRAPPC4 on HeLa cell. [antibody concentration 30ug/ml].)

WB (Western Blot) (TRAPPC4 monoclonal antibody. Western Blot analysis of TRAPPC4 expression in Raw 264.7.)

WB (Western Blot) (TRAPPC4 monoclonal antibody, Western Blot analysis of TRAPPC4 expression in HeLa.)

WB (Western Blot) (Western Blot detection against Immunogen (50.2kD).)

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TRAPPC4, Monoclonal Antibody (Cat# AAA25572)

Full Name

TRAPPC4 (Trafficking Protein Particle Complex Subunit 4, Synbindin, TRS23 Homolog, Hematopoietic Stem/Progenitor Cell Protein 172, SBDN, CGI-104, HSPC172, PTD009) (HRP)

Gene Names

TRAPPC4; SBDN; TRS23; PTD009; CGI-104; HSPC172; SYNBINDIN

Reactivity

Human, Mouse

Applications

EIA, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (RAB7B monoclonal antibody. Western Blot analysis of RAB7B expression in A-431.)

Application Data (Detection limit for recombinant GST tagged RAB7B is ~0.03ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of RAB7B transfected lysate using RAB7B monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with RAB7B rabbit polyclonal antibody.)

WB (Western Blot) (Western Blot analysis of RAB7B expression in transfected 293T cell line by RAB7B monoclonal antibody. Lane 1: RAB7B transfected lysate (22.5kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (RAB7B monoclonal antibody. Western Blot analysis of RAB7B expression in human kidney.)

WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)

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RAB7B, Monoclonal Antibody (Cat# AAA25515)

Full Name

RAB7B (Ras-related Protein Rab-7b, RAB7) (HRP)

Gene Names

RAB7B; RAB7

Reactivity

Human

Applications

EIA, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged FGR is ~0.3ng/ml as a capture antibody.)

IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to FGR on formalin-fixed paraffin-embedded human spleen. [antibody concentration 3ug/ml.)

WB (Western Blot) (Western Blot analysis of FGR expression in transfected 293T cell line by FGR monoclonal antibody. Lane 1: FGR transfected lysate (59.5kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (FGR monoclonal antibody. Western Blot analysis of FGR expression in Raw 264.7.)

WB (Western Blot) (FGR monoclonal antibody. Western Blot analysis of FGR expression in PC-12.)

WB (Western Blot) (FGR monoclonal antibody. Western Blot analysis of FGR expression in HeLa.)

WB (Western Blot) (Western Blot detection against Immunogen (35.53kD).)

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FGR, Monoclonal Antibody (Cat# AAA24801)

Full Name

FGR (Tyrosine-protein Kinase Fgr, Gardner-Rasheed Feline Sarcoma Viral (v-fgr) Oncogene Homolog, Proto-oncogene c-Fgr, p55-Fgr, p58-Fgr, p58c-Fgr, SRC2) (Biotin)

Gene Names

FGR; SRC2; c-fgr; c-src2; p55-Fgr; p58-Fgr; p55c-fgr; p58c-fgr

Reactivity

Human, Mouse, Rat

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TOMM20 on HeLa cell. [antibody concentration 10ug/ml])

Application Data (Detection limit for recombinant GST tagged TOMM20 is ~0.1ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase on formalin-fixed paraffin-embedded human small Intestine using 1346004 (3ug/ml).)

WB (Western Blot) (Western Blot analysis in transfected 293T cell line using 134604. Lane 1: 134604 transfected lysate (16.3kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot analysis in NIH/3T3 using 134604.)

WB (Western Blot) (Western Blot analysis of in PC-12 using 134604.)

WB (Western Blot) (Western Blot analysis in HeLa using 134604.)

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TOMM20, Monoclonal Antibody (Cat# AAA24980)

Full Name

TOMM20 (Mitochondrial Import Receptor Subunit TOM20 Homolog, TOM20, Mitochondrial 20kD Outer Membrane Protein, Outer Mitochondrial Membrane Receptor Tom20, KIAA0016, MAS20, MOM19)

Gene Names

TOMM20; MAS20; MOM19; TOM20

Reactivity

Human, Mouse, Rat

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western Blot detection against Immunogen (36.63kD).)

Application Data (Detection limit for recombinant GST tagged MAGEA12 is 0.03ng/ml as a capture antibody.)

WB (Western Blot) (MAGEA12 monoclonal antibody. Western Blot analysis of MAGEA12 expression in NIH/3T3.)

WB (Western Blot) (MAGEA12 monoclonal antibody. Western Blot analysis of MAGEA12 expression in Jurkat.)

WB (Western Blot) (MAGEA12 monoclonal antibody. Western Blot analysis of MAGEA12 expression in Raw 264.7.)

WB (Western Blot) (MAGEA12 monoclonal antibody. Western Blot analysis of MAGEA12 expression in human liver.)

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MAGEA12, Monoclonal Antibody (Cat# AAA14797)

Full Name

MAGEA12 (Melanoma-associated Antigen 12, MAGE-12 Antigen, MAGE-12F Antigen, Cancer/Testis Antigen 1.12, CT1.12, MAGE12)

Gene Names

MAGEA12; CT1.12; MAGE12

Reactivity

Human, Mouse

Applications

EL/EIA, WB

Purity

Affinity Purified
Purified by Protein A affinity chromatography.

Pricing

$820/0.1 mg $1,565/2x0.1 mg $2,270/3x0.1 mg $2,940/4x0.1 mg $3,380/5x0.1 mg

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WB (Western Blot) (MKRN2 monoclonal antibody Western Blot analysis of MKRN2 expression in Hela NE.)

WB (Western Blot) (Western blot analysis of MKRN2 over-expressed 293 cell line, cotransfected with MKRN2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with MKRN2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged MKRN2 is ~0.1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MKRN2 on HeLa cell. [antibody concentration 10ug/ml])

WB (Western Blot) (Western Blot analysis of MKRN2 expression in transfected 293T cell line by MKRN2 monoclonal antibody. Lane 1: MKRN2 transfected lysate (46.9kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (37kD).)

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MKRN2, Monoclonal Antibody (Cat# AAA24276)

Full Name

MKRN2 (RNF62, Probable E3 Ubiquitin-protein Ligase Makorin-2, RING Finger Protein 62, HSPC070) (AP)

Gene Names

MKRN2; RNF62; HSPC070

Reactivity

Human

Applications

EIA, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to GABPA on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to GABPA on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml])

Application Data (Detection limit for recombinant GST tagged GABPA is approximately 3ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to GABPA on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to GABPA on HeLa cell. [antibody concentration 10 ug/ml])

WB (Western Blot) (GABPA monoclonal antibody (M07), clone 5C8 Western Blot analysis of GABPA expression in Hela S3 NE (Cat # L013V3).)

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GABPA, Monoclonal Antibody (Cat# AAA26108)

Full Name

GABPA (GA Binding Protein Transcription Factor, alpha Subunit 60kD, E4TF1-60, E4TF1A, NFT2, NRF2, NRF2A) (APC)

Gene Names

GABPA; NFT2; NRF2; NRF2A; E4TF1A; E4TF1-60; RCH04A07

Applications

IF, IHC, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

WB (Western Blot) (Western Blot detection against Immunogen (31.61kD).)

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EHD3, Monoclonal Antibody (Cat# AAA24197)

Full Name

EHD3 (EH Domain-containing Protein 3, PAST Homolog 3, EHD2, PAST3) (AP)

Gene Names

EHD3; PAST3

Reactivity

Human

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RUNX2 on U-2 OS cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RUNX2 on U-2 OS cell. [antibody concentration 10 ug/ml])

Application Data (Detection limit for recombinant GST tagged RUNX2 is approximately 10ng/ml as a capture antibody.)

WB (Western Blot) (RUNX2 monoclonal antibody (M04), clone 4D5 Western Blot analysis of RUNX2 expression in PC-12.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to RUNX2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml])

Application Data (Immunoperoxidase of monoclonal antibody to RUNX2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml])

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RUNX2, Monoclonal Antibody (Cat# AAA26111)

Full Name

RUNX2 (Runt-Related Transcription Factor 2, AML3, CBFA1, CCD, CCD1, MGC120022, MGC120023, OSF2, PEA2aA, PEBP2A1, PEBP2A2, PEBP2aA, PEBP2aA1) (APC)

Gene Names

RUNX2; CCD; AML3; CCD1; CLCD; OSF2; CBFA1; OSF-2; PEA2aA; PEBP2aA; CBF-alpha-1

Applications

IF, IHC, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between CASP3 and HCLS1. HeLa cells were stained with anti-CASP3 rabbit purified polyclonal 1:1200 and anti-HCLS1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

WB (Western Blot) (Western blot analysis of HCLS1 over-expressed 293 cell line, cotransfected with HCLS1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with HCLS1 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged HCLS1 is ~0.1ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HCLS1 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot analysis of HCLS1 expression in transfected 293T cell line by HCLS1 monoclonal antibody. Lane 1: HCLS1 transfected lysate (54kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (HCLS1 monoclonal antibody Western Blot analysis of HCLS1 expression in K-562.)

WB (Western Blot) (Western Blot detection against Immunogen (35.64kD).)

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HCLS1, Monoclonal Antibody (Cat# AAA24234)

Full Name

HCLS1 (Hematopoietic Lineage Cell-specific Protein, Hematopoietic Cell-specific LYN Substrate 1, LckBP1, p75, HS1) (AP)

Gene Names

HCLS1; HS1; p75; CTTNL; lckBP1

Reactivity

Human

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged HIP1 is ~0.3ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of HIP1 transfected lysate using HIP1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HIP1 rabbit polyclonal antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HIP1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot analysis of HIP1 expression in transfected 293T cell line by HIP1 monoclonal antibody Lane 1: HIP1 transfected lysate (116.2kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (HIP1 monoclonal antibody Western Blot analysis of HIP1 expression in HeLa)

WB (Western Blot) (Western Blot detection against Immunogen (37.84kD).)

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HIP1, Monoclonal Antibody (Cat# AAA24828)

Full Name

HIP1 (Huntingtin-interacting Protein 1, Huntingtin-interacting Protein I, MGC126506) (Biotin)

Gene Names

HIP1; SHON; HIP-I; ILWEQ; SHONbeta; SHONgamma

Reactivity

Human

Applications

EIA, IHC, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of A431 cells using anti-Cytokeratin 5 antibody (AAA19666).Overlay histogram showing A431 cells stained with AAA19666 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Cytokeratin 5 Antibody (AAA19666, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 5. IF analysis of Cytokeratin 5 using anti-Cytokeratin 5 antibody (AAA19666).Cytokeratin 5 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL mouse anti-Cytokeratin 5 Antibody (AAA19666) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of Cytokeratin 5 using anti-Cytokeratin 5 antibody (AAA19666).Cytokeratin 5 was detected in a paraffin-embedded section of human rectal moderately differentiated adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Cytokeratin 5 Antibody (AAA19666) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of Cytokeratin 5 using anti-Cytokeratin 5 antibody (AAA19666).Cytokeratin 5 was detected in a paraffin-embedded section of human metaplasia of squamous cells of the renal pelvis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Cytokeratin 5 Antibody (AAA19666) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of Cytokeratin 5 using anti-Cytokeratin 5 antibody (AAA19666).Cytokeratin 5 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Cytokeratin 5 Antibody (AAA19666) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of Cytokeratin 5 using anti-Cytokeratin 5 antibody (AAA19666).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human A431 whole cell lysates,Lane 2: human SH-SY5Y whole cell lysates,Lane 3: human Hacat whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Cytokeratin 5 antigen affinity purified monoclonal antibody (#AAA19666) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Cytokeratin 5 at approximately 62 kDa. The expected band size for Cytokeratin 5 is at 62 kDa.)

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Cytokeratin 5, Monoclonal Antibody (Cat# AAA19666)

Full Name

Anti-Cytokeratin 5 Antibody Picoband (monoclonal, 9H2F8)

Gene Names

KRT5; K5; CK5; DDD; EBS2; KRT5A

Reactivity

Human

Applications

WB, IHC, ICC, IF, FC/FACS

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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WB (Western Blot) (EIF5 monoclonal antibody, Western Blot analysis of EIF5 expression in Jurkat.)

Application Data (Detection limit for recombinant GST tagged EIF5 is ~1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to EIF5 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to EIF5 on formalin-fixed paraffin-embedded human malignant lymphoma, diffuse large B tissue. [antibody concentration 1ug/ml].)

WB (Western Blot) (Western Blot analysis of EIF5 expression in transfected 293T cell line by EIF5 monoclonal antibody. Lane 1: EIF5 transfected lysate (49.2kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (73.15kD).)

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EIF5, Monoclonal Antibody (Cat# AAA24201)

Full Name

EIF5 (Eukaryotic Translation Initiation Factor 5, eIF-5) (AP)

Gene Names

EIF5; EIF-5; EIF-5A

Reactivity

Human

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged SRPK1 is ~0.03ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SRPK1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 1ug/ml].)

WB (Western Blot) (Western Blot analysis of SRPK1 expression in transfected 293T cell line by SRPK1 monoclonal antibody. Lane 1: SRPK1 transfected lysate (74.3kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (SRPK1 monoclonal antibody, Western Blot analysis of SRPK1 expression in IMR-32.)

WB (Western Blot) (SRPK1 monoclonal antibody. Western Blot analysis of SRPK1 expression in HepG2.)

WB (Western Blot) (Western Blot detection against Immunogen (37kD).)

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SRPK1, Monoclonal Antibody (Cat# AAA25853)

Full Name

SRPK1 (SRSF Protein Kinase 1, SFRS Protein Kinase 1, Serine/Arginine-rich Protein-specific Kinase 1, SR-protein-specific Kinase 1) (PE)

Reactivity

Human

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of MPP1 expression in transfected 293T cell line by MPP1 monoclonal antibody (M01), clone 1E11-1G11.Lane 1: MPP1 transfected lysate (Predicted MW: 52.3 KDa).Lane 2: Non-transfected lysate.)

IP (Immunoprecipitation) (Immunoprecipitation of MPP1 transfected lysate using anti-MPP1 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with MPP1 monoclonal antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MPP1 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MPP1 on HeLa cell. [antibody concentration 10 ug/ml])

WB (Western Blot) (MPP1 monoclonal antibody (M01), clone 1E11-1G11. Western Blot analysis of MPP1 expression in human placenta.)

Application Data (Detection limit for recombinant GST tagged MPP1 is approximately 1ng/ml as a capture antibody.)

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MPP1, Monoclonal Antibody (Cat# AAA26345)

Full Name

MPP1 (Membrane Protein, Palmitoylated 1, 55kD, AAG12, DXS552, DXS552E, EMP55, MRG1, PEMP) (FITC)

Gene Names

MPP1; MRG1; PEMP; AAG12; EMP55; DXS552E

Applications

IF, IP, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged TP53 is approximately 3ng/ml as a capture antibody.)

WB (Western Blot) (TP53 monoclonal antibody, Western Blot analysis of TP53 expression in A-431.)

WB (Western Blot) (Western Blot detection against Immunogen (37.62kD.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TP53 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 6ug/ml])

Application Data (Proximity Ligation Analysis of protein-protein interactions between TP53 and BAX. HeLa cells were stained with TP53 rabbit purified polyclonal 1:1200 and BAX mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)

WB (Western Blot) (Western Blot analysis of TP53 expression in transfected 293T cell line by TP53 monoclonal antibody. Lane 1: TP53 transfected lysate (43.34kD) Lane 2: Non-transfected lysate.)

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TP53, Monoclonal Antibody (Cat# AAA24982)

Full Name

TP53 (Tumor Suppressor p53, Cellular Tumor Antigen p53, TRP53, Antigen NY-CO-13, BCC7, LFS1, Phosphoprotein p53) (Biotin)

Gene Names

TP53; P53; BCC7; LFS1; TRP53

Reactivity

Human

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged FOXA1 is approximately 0.03ng/ml as a capture antibody.)

WB (Western Blot) (FOXA1 monoclonal antibody (M02), clone 1B1 Western Blot analysis of FOXA1 expression in HepG2.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FOXA1 on HepG2 cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FOXA1 on HepG2 cell. [antibody concentration 10 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to FOXA1 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 ug/ml])

Application Data (Immunoperoxidase of monoclonal antibody to FOXA1 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 ug/ml])

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FOXA1, Monoclonal Antibody (Cat# AAA26083)

Full Name

FOXA1 (Forkhead Box A1, HNF3A, MGC33105, TCF3A) (APC)

Gene Names

FOXA1; HNF3A; TCF3A

Applications

IF, IHC, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 WB 1:500-2000,IHC-p 1:50-300)

IHC (Immunohistchemistry) (Dilution: IF: 1:50-200 WB 1:500-2000,IHC-p 1:50-300)

Application Data (Dilution: IF: 1:50-200 WB 1:500-2000,IHC-p 1:50-300)

Application Data (Dilution: IF: 1:50-200 WB 1:500-2000,IHC-p 1:50-300)

Application Data (Dilution: IF: 1:50-200 WB 1:500-2000,IHC-p 1:50-300)

IHC (Immunohistochemistry-Paraffin) (Dilution: IF: 1:50-200 WB 1:500-2000,IHC-p 1:50-300)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 WB 1:500-2000,IHC-p 1:50-300)

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TTR, Monoclonal Antibody (Cat# AAA28913)

Full Name

TTR mouse Monoclonal Antibody (1D7)

Gene Names

TTR; CTS; CTS1; PALB; TBPA; HsT2651

Reactivity

Human

Applications

WB, IHC-P, IF

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

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WB (Western Blot) (Western blot analysis of ABL2 over-expressed 293 cell line, cotransfected with ABL2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ABL2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between NCK1 and ABL2. HeLa cells were stained with NCK1 rabbit purified polyclonal 1:1200 and ABL2 mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

Application Data (Detection limit for recombinant GST tagged ABL2 is ~0.03ng/ml as a capture antibody.)

WB (Western Blot) (Western Blot analysis of ABL2 expression in transfected 293T cell line by ABL2 monoclonal antibody. Lane 1: ABL2 transfected lysate (126.7kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (ABL2 monoclonal antibody Western Blot analysis of ABL2 expression in Hela NE.)

WB (Western Blot) (Western Blot detection against Immunogen (37kD).)

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ABL2, Monoclonal Antibody (Cat# AAA25592)

Full Name

ABL2 (Tyrosine-protein Kinase ABL2, Abelson Murine Leukemia Viral Oncogene Homolog 2, Abelson-related Gene Protein, Tyrosine Kinase ARG, ABLL, ARG) (PE)

Gene Names

ABL2; ARG; ABLL

Reactivity

Human

Applications

EIA, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IF (Immunofluorescence) (Figure 11. IF analysis of Tubulin alpha using anti- Tubulin alpha antibody (AAA19381).Tubulin alpha was detected in immunocytochemical section of CACO-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL mouse anti- Tubulin alpha Antibody (AAA19381) overnight at 4 degree C. DyLight®594 Conjugated Goat Anti-Mouse IgG (BA1141) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of A431 cells using anti-Tubulin alpha antibody (AAA19381).Overlay histogram showing A431 cells stained with AAA19381 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- Tubulin alpha Antibody (AAA19381, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 9. IF analysis of Tubulin alpha using anti- Tubulin alpha antibody (AAA19381).Tubulin alpha was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL mouse anti- Tubulin alpha Antibody (AAA19381) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 8. IHC analysis of Tubulin alpha using anti-Tubulin alpha antibody (AAA19381).Tubulin alpha was detected in paraffin-embedded section of human pancreatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Tubulin alpha Antibody (AAA19381) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of Tubulin alpha using anti-Tubulin alpha antibody (AAA19381).Tubulin alpha was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Tubulin alpha Antibody (AAA19381) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of Tubulin alpha using anti-Tubulin alpha antibody (AAA19381).Tubulin alpha was detected in paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Tubulin alpha Antibody (AAA19381) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of Tubulin alpha using anti-Tubulin alpha antibody (AAA19381).Tubulin alpha was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Tubulin alpha Antibody (AAA19381) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of Tubulin alpha using anti-Tubulin alpha antibody (AAA19381).Tubulin alpha was detected in paraffin-embedded section of human ovarian serous adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Tubulin alpha Antibody (AAA19381) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of Tubulin alpha using anti-Tubulin alpha antibody (AAA19381).Tubulin alpha was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Tubulin alpha Antibody (AAA19381) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of Tubulin alpha using anti-Tubulin alpha antibody (AAA19381).Tubulin alpha was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Tubulin alpha Antibody (AAA19381) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of Tubulin alpha using anti-Tubulin alpha antibody (AAA19381).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HELA whole cell lysatesLane 2: human Jurkat whole cell lysatesLane 3: human HEK293 whole cell lysatesLane 4: human K562 whole cell lysatesLane 5: rat brain tissue lysatesLane 6: rat C6 whole cell lysatesLane 7: mouse brain tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- Tubulin alpha antigen affinity purified monoclonal antibody (Catalog # AAA19381) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for Tubulin alpha at approximately 56KD. The expected band size for Tubulin alpha is at 56KD.)

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Tubulin alpha, Monoclonal Antibody (Cat# AAA19381)

Full Name

Anti-Tubulin alpha Antibody (monoclonal, 7B12)

Gene Names

TUBA1A; LIS3; TUBA3; B-ALPHA-1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, ICC, IF, FC/FACS/FCM

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to GSTZ1 on HepG2 cell. [antibody concentration 10ug/ml].)

WB (Western Blot) (Western Blot analysis of GSTZ1 expression in transfected 293T cell line by GSTZ1 monoclonal antibody. Lane 1: GSTZ1 transfected lysate (24.1kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (GSTZ1 monoclonal antibody. Western Blot analysis of GSTZ1 expression in NIH/3T3.)

WB (Western Blot) (GSTZ1 monoclonal antibody, Western Blot analysis of GSTZ1 expression in HepG2.)

WB (Western Blot) (GSTZ1 monoclonal antibody. Western Blot analysis of GSTZ1 expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (37.62kD).)

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GSTZ1, Monoclonal Antibody (Cat# AAA24822)

Full Name

GSTZ1 (Glutathione S-transferase zeta 1, Glutathione S-Transferase zeta 1, GSTZ1-1, Maleylacetoacetate Isomerase, MAAI, MAI, MGC2029) (Biotin)

Gene Names

GSTZ1; MAI; MAAI; MAAID; GSTZ1-1

Reactivity

Human, Mouse, Rat

Applications

EIA, IF, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IF (Immunofluorescence) (Figure 6. IF analysis of Filamin B/FLNB using anti-Filamin B/FLNB antibody (AAA19696).Filamin B/FLNB was detected in an immunocytochemical section of HeLa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL mouse anti-Filamin B/FLNB Antibody (AAA19696) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of Filamin B/FLNB using anti-Filamin B/FLNB antibody (AAA19696).Filamin B/FLNB was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Filamin B/FLNB Antibody (AAA19696) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of Filamin B/FLNB using anti-Filamin B/FLNB antibody (AAA19696).Filamin B/FLNB was detected in a paraffin-embedded section of human metaplasia of squamous cells of the renal pelvis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Filamin B/FLNB Antibody (AAA19696) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of Filamin B/FLNB using anti-Filamin B/FLNB antibody (AAA19696).Filamin B/FLNB was detected in a paraffin-embedded section of human lymph node of rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Filamin B/FLNB Antibody (AAA19696) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of Filamin B/FLNB using anti-Filamin B/FLNB antibody (AAA19696).Filamin B/FLNB was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Filamin B/FLNB Antibody (AAA19696) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of Filamin B/FLNB using anti-Filamin B/FLNB antibody (AAA19696).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human A549 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Filamin B/FLNB antigen affinity purified monoclonal antibody (#AAA19696) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Filamin B/FLNB at approximately 278 kDa. The expected band size for Filamin B/FLNB is at 278 kDa.)

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Filamin B/FLNB, Monoclonal Antibody (Cat# AAA19696)

Full Name

Anti-Filamin B/FLNB Antibody Picoband (monoclonal, 11E2D2)

Gene Names

FLNB; AOI; FH1; SCT; TAP; LRS1; TABP; FLN-B; FLN1L; ABP-278; ABP-280

Reactivity

Human

Applications

WB, IHC, ICC, IF

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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Application Data (Detection limit for recombinant GST tagged SORD is ~0.1ng/ml as a capture antibody.)

IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to SORD on formalin-fixed paraffin-embedded human kidney. [antibody concentration 1.2ug/ml].)

WB (Western Blot) (Western Blot analysis of SORD expression in transfected 293T cell line by SORD monoclonal antibody. Lane 1: SORD transfected lysate (38.165kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (SORD monoclonal antibody. Western Blot analysis of SORD expression in Raw 264.7.)

WB (Western Blot) (SORD monoclonal antibody, Western Blot analysis of SORD expression in Hela NE.)

WB (Western Blot) (SORD monoclonal antibody. Western Blot analysis of SORD expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (38.21kD).)

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SORD, Monoclonal Antibody (Cat# AAA25553)

Full Name

SORD (Sorbitol Dehydrogenase, L-iditol 2-dehydrogenase) (HRP)

Gene Names

SORD; SORD1; HEL-S-95n

Reactivity

Human, Mouse, Rat

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged NDUFA9 is ~0.1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NDUFA9 on NIH/3T3 cell. [antibody concentration 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NDUFA9 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 0.8ug/ml])

WB (Western Blot) (NDUFA9 monoclonal antibody Western Blot analysis of NDUFA9 expression in NIH/3T3.)

WB (Western Blot) (NDUFA9 monoclonal antibody. Western Blot analysis of NDUFA9 expression in Raw 264.7.)

WB (Western Blot) (NDUFA9 monoclonal antibody Western Blot analysis of NDUFA9 expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (33.99kD).)

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NDUFA9, Monoclonal Antibody (Cat# AAA24284)

Full Name

NDUFA9 (NADH Dehydrogenase [Ubiquinone] 1 alpha Subcomplex Subunit 9, Mitochondrial, NADH-Ubiquinone Oxidoreductase 39kD Subunit, Complex I-39kD, CI-39kD, NDUFS2L, MGC111043) (AP)

Gene Names

NDUFA9; CC6; CI39k; CI-39k; MC1DN26; NDUFS2L; SDR22E1

Reactivity

Human, Mouse, Rat

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western blot analysis of IRAK1 over-expressed 293 cell line, cotransfected with IRAK1 Validated Chimera RNAi ((Lane 2) or non-transfected control (Lane 1). Blot probed with IRAK1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged IRAK1 is ~0.03ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of IRAK1 transfected lysate using IRAK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with IRAK1 rabbit polyclonal antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to IRAK1 on HeLa cell. [antibody concentration 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to IRAK1 on formalin-fixed paraffin-embedded human lung. [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot detection against Immunogen (43.45kD).)

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IRAK1, Monoclonal Antibody (Cat# AAA24544)

Full Name

IRAK1 (Interleukin-1 Receptor-associated Kinase 1, IRAK-1, IRAK) APC

Gene Names

IRAK1; IRAK; pelle

Reactivity

Human

Applications

EIA, IF, IHC, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged HDAC1 is approximately 0.1ng/ml as a capture antibody.)

WB (Western Blot) (HDAC1 monoclonal antibody (M02), clone 3E1 Western Blot analysis of HDAC1 expression in Hela S3 NE (Cat # L013V3).)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HDAC1 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HDAC1 on HeLa cell. [antibody concentration 10 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HDAC1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml])

Application Data (Immunoperoxidase of monoclonal antibody to HDAC1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml])

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HDAC1, Monoclonal Antibody (Cat# AAA26597)

Full Name

HDAC1 (Histone Deacetylase 1, DKFZp686H12203, GON-10, HD1, RPD3, RPD3L1) (PE)

Gene Names

HDAC1; HD1; RPD3; KDAC1; GON-10; RPD3L1

Applications

IF, IHC, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western blot analysis of PIP5K3 over-expressed 293 cell line, cotransfected with PIP5K3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PIP5K3 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged PIP5K3 is ~0.3ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PIP5K3 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PIP5K3 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of PIP5K3 expression in transfected 293T cell line by PIP5K3 monoclonal antibody. Lane 1: PIP5K3 transfected lysate (50.2kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (38.21kD).)

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PIP5K3, Monoclonal Antibody (Cat# AAA25795)

Full Name

PIP5K3 (PIKFYVE, 1-phosphatidylinositol 3-phosphate 5-kinase, Phosphatidylinositol 3-phosphate 5-kinase, FYVE Finger-containing Phosphoinositide Kinase, PIKfyve, Phosphatidylinositol 3-phosphate 5-kinase Type III, PIPkin-III, Type III PIP Kinase, KIAA0981

Gene Names

PIKFYVE; CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29

Reactivity

Human

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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FCM (Flow Cytometry) (Figure 11. Flow Cytometry analysis of HL-60 cells using anti-MCM6 antibody (AAA19704).Overlay histogram showing HL-60 cells stained with AAA19704 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-MCM6 Antibody (AAA19704, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 10. IF analysis of MCM6 using anti-MCM6 antibody (AAA19704).MCM6 was detected in an immunocytochemical section of CACO-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL mouse anti-MCM6 Antibody (AAA19704) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistchemistry) (Figure 9. IHC analysis of MCM6 using anti-MCM6 antibody (AAA19704).MCM6 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-MCM6 Antibody (AAA19704) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 8. IHC analysis of MCM6 using anti-MCM6 antibody (AAA19704).MCM6 was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-MCM6 Antibody (AAA19704) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of MCM6 using anti-MCM6 antibody (AAA19704).MCM6 was detected in a paraffin-embedded section of human rectal moderately differentiate dadenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-MCM6 Antibody (AAA19704) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of MCM6 using anti-MCM6 antibody (AAA19704).MCM6 was detected in a paraffin-embedded section of human metaplasia of squamous cells of the renal pelvis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-MCM6 Antibody (AAA19704) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of MCM6 using anti-MCM6 antibody (AAA19704).MCM6 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-MCM6 Antibody (AAA19704) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of MCM6 using anti-MCM6 antibody (AAA19704).MCM6 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-MCM6 Antibody (AAA19704) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of MCM6 using anti-MCM6 antibody (AAA19704).MCM6 was detected in a paraffin-embedded section of human bladder epithelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-MCM6 Antibody (AAA19704) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of MCM6 using anti-MCM6 antibody (AAA19704).MCM6 was detected in a paraffin-embedded section of human Hodgkin's lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-MCM6 Antibody (AAA19704) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of MCM6 using anti-MCM6 antibody (AAA19704).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human Jurkat whole cell lysates,Lane 3: human 293T whole cell lysates,Lane 4: rat RH35 whole cell lysates,Lane 5: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-MCM6 antigen affinity purified monoclonal antibody (#AAA19704) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MCM6 at approximately 105 kDa. The expected band size for MCM6 is at 93 kDa.)

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MCM6, Monoclonal Antibody (Cat# AAA19704)

Full Name

Anti-MCM6 Antibody Picoband (monoclonal, 3F13C4)

Gene Names

MCM6; Mis5; P105MCM; MCG40308

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ICC, IF, FC/FACS

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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WB (Western Blot) (Western Blot Analysis of human liver tissue lysate using Prohibitin Mouse Monoclonal Antibody (PHB/3225).)

Application Data (Analysis of Protein Array containing more than 19,000 full-length human proteins using Monospecific Mouse Monoclonal Antibody to Prohibitin (PHB/3225). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

IF (Immunofluorescence) (Confocal Immunofluorescence of HepG2 cells stained with Prohibitin Mouse Monoclonal Antibody (PHB/3225) labeled with CF488 (Green); Reddot is used to label the nuclei.)

SDS-PAGE (SDS-PAGE Analysis Purified Prohibitin Mouse Monoclonal Antibody (PHB/3225). Confirmation of Purity and Integrity of Antibody.)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Liver stained with Prohibitin Mouse Monoclonal Antibody (PHB/3225).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Liver stained with Prohibitin Mouse Monoclonal Antibody (PHB/3225).)

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Prohibitin, Monoclonal Antibody (Cat# AAA23933)

Full Name

Prohibitin (Mitochondrial Marker)

Gene Names

PHB; PHB1; HEL-215; HEL-S-54e

Reactivity

Human

Applications

WB, IF, IHC

Purity

Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Pricing

$295/0.02 mg (With BSA & Azide at 0.2mg/mL) $475/0.1 mg (With BSA & Azide at 0.2mg/mL) $475/0.1 mg (Without BSA & Azide at 1mg/mL) $905/2x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,310/3x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,690/4x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,900/5x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,900/5x0.1 mg (Without BSA & Azide at 1mg/mL)

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WB (Western Blot) (ACSL5 monoclonal antibody, Western Blot analysis of ACSL5 expression in HepG2.)

Application Data (Detection limit for recombinant GST tagged ACSL5 is ~0.1ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of ACSL5 transfected lysate using ACSL5 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ACSL5 rabbit polyclonal antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ACSL5 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of ACSL5 expression in transfected 293T cell line by ACSL5 monoclonal antibody. Lane 1: ACSL5 transfected lysate (82.3kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (36.67kD).)

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ACSL5, Monoclonal Antibody (Cat# AAA25007)

Full Name

ACSL5 (Long-chain-fatty-acid-CoA Ligase 5, Long-chain acyl-CoA Synthetase 5, LACS 5, ACS5, FACL5, UNQ633/PRO1250) (FITC)

Gene Names

ACSL5; ACS2; ACS5; FACL5

Reactivity

Human

Applications

EIA, IHC, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IHC (Immunohistchemistry) (PINK1 Monoclonal Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PINK1 Monoclonal Antibody for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot) (Western blot analysis of PINK (arrow) using mouse monoclonal PINK antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PINK gene (Lane 2) (Origene Technologies))

WB (Western Blot) (Western blot analysis of lysates from A431 cell line, mouse brain tissue lysate(from left to right), using Pink1 Antibody(115-213). AAA28638 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)

IF (Immunofluorescence) (Fluorescent image of PC12 cells stained with Pink1(115-213) Antibody. AAA28638 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded H. heart section using Pink1(115-213). AAA28638 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded H. stomach section using Pink1(115-213). AAA28638 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)

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PINK1, Monoclonal Antibody (Cat# AAA28638)

Full Name

PINK1 Antibody

Gene Names

PINK1; BRPK; PARK6

Reactivity

Human

Applications

EIA, IHC, IF, WB

Purity

Purified Mouse Monoclonal Antibody (Mab)

Pricing

$275/0.05 mL $510/0.2 mL $975/2x0.2 mL $1,415/3x0.2 mL $1,825/4x0.2 mL $2,070/5x0.2 mL

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WB (Western Blot) (CCNH monoclonal antibody, Western Blot analysis of CCNH expression in HeLa.)

Application Data (Detection limit for recombinant GST tagged CCNH is ~1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CCNH on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CCNH on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of CCNH expression in transfected 293T cell line by CCNH monoclonal antibody. Lane 1: CCNH transfected lysate (37.6kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (37.73kD).)

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CCNH, Monoclonal Antibody (Cat# AAA24748)

Full Name

CCNH (Cyclin H, Cyclin-H, MO15-associated Protein, p34, p37) (Biotin)

Gene Names

CCNH; CAK; p34; p37; CycH

Reactivity

Human

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (HHIP monoclonal antibody. Western Blot analysis of HHIP expression in MCF-7.)

WB (Western Blot) (HHIP monoclonal antibody, Western Blot analysis of HHIP expression in Hela NE.)

Application Data (Detection limit for recombinant GST tagged HHIP is ~1ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of HHIP transfected lysate using HHIP monoclonal antibody and Protein A Magnetic Bead and immunoblotted with HHIP rabbit polyclonal antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HHIP on formalin-fixed paraffin-embedded human heart. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of HHIP expression in transfected 293T cell line by HHIP monoclonal antibody. Lane 1: HHIP transfected lysate (78.9kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)

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HHIP, Monoclonal Antibody (Cat# AAA24827)

Full Name

HHIP (Hedgehog-interacting Protein, HHIP, HIP, UNQ5825/PRO19644, FLJ20992, FLJ90230) (Biotin)

Gene Names

HHIP; HIP

Reactivity

Human

Applications

EIA, IHC, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (FGR monoclonal antibody (M02), clone 3B11. Western Blot analysis of FGR expression in Raw 264.7.)

WB (Western Blot) (FGR monoclonal antibody (M02), clone 3B11. Western Blot analysis of FGR expression in PC-12.)

Application Data (Detection limit for recombinant GST tagged FGR is approximately 0.1ng/ml as a capture antibody.)

WB (Western Blot) (FGR monoclonal antibody (M02), clone 3B11 Western Blot analysis of FGR expression in HeLa.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to FGR on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 3 ug/ml])

Application Data (Immunoperoxidase of monoclonal antibody to FGR on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 3 ug/ml])

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FGR, Monoclonal Antibody (Cat# AAA26026)

Full Name

FGR (Gardner-Rasheed feline Sarcoma Viral (v-fgr) Oncogene Homolog, FLJ43153, MGC75096, SRC2, c-fgr, c-src2, p55c-fgr, p58c-fgr) (APC)

Gene Names

FGR; SRC2; c-fgr; c-src2; p55-Fgr; p58-Fgr; p55c-fgr; p58c-fgr

Applications

IHC, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western blot analysis of RAD51C over-expressed 293 cell line, cotransfected with RAD51C Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with RAD51C monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged RAD51C is ~0.1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RAD51C on HeLa cell. [antibody concentration 10ug/ml])

WB (Western Blot) (Western Blot analysis of RAD51C expression in transfected 293T cell line by RAD51C monoclonal antibody. Lane 1: RAD51C transfected lysate (42.2kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (RAD51C monoclonal antibody. Western Blot analysis of RAD51C expression in 293.)

WB (Western Blot) (RAD51C monoclonal antibody, Western Blot analysis of RAD51C expression in HeLa.)

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RAD51C, Monoclonal Antibody (Cat# AAA25517)

Full Name

RAD51C (DNA Repair Protein RAD51 Homolog 3, R51H3, RAD51 Homolog C, RAD51-like Protein 2, MGC104277, RAD51L2) (HRP)

Gene Names

RAD51C; FANCO; R51H3; BROVCA3; RAD51L2

Reactivity

Human

Applications

EIA, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

What are Monoclonal Antibodies?

Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.

This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.

AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.

What Are The Uses of Monoclonal Antibodies

Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.

ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.

Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.

Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.

Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.

How Monoclonal Antibodies Are Used as Medicine?

Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).

Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:

• Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.


• Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.


• Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.

Why Buy Monoclonal Antibodies From Us?

At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.

1. Validated for Versatile Applications

The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).

2. Wide Selection & Specialized Options

We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.

3. High-Quality Proteins

Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.

Frequently Asked Questions

1. Are your monoclonal antibodies validated for specific applications?

Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.

2. How do I choose the right monoclonal antibody for my application?

Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.

3. How quickly can I receive my order?

Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.